Rorug06G0338900

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006256
Physical Location & Seq
Forward (+)
49060631 .. 49063487
2857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0338900.1

Sequence Viewer

Length: 1584 bp
ATGGAAGAGAAAAGAAGTTGGAACTTCAAAAACCCCAAAACCCTTAAATGCAAATCAAAGCAACCTATCTCCTCTGACCCAATCACATATTCAAACCCTCTCTACTCTTCACTCCCAACTGAAAGCTTCGCTGGGTTTCACAATTCAAACCCAGAATCACAACCCACACAGATGACGGGGCTCGTAATGGTGACGAGGGGCGGTGGTGGTTGCAAAGAGGGGAAGAAGAGCTCGGAAGAAGAGCAAAACCAGCTCTCTCTGGTGGATTTTCTGTTGGCCGCGGTGAGGAAATCCATGGTGGCCTGCCGCGTGGAGCGAGGCGAGGAGGTGATCTCCGCCGTCAATAATATGGAGATCGGGTGGCCCACGAATGTCCGGCATGTGACGCATGTCACGTTCGACCGGTTCAACGGGTTTCTGGGTCTTCCGGTGGAGTTCGAGGTCGATGTCCCGGGTCGGGTTCCGAGTGCTAGTGCAAGTGTGTTTGGGGTCTCAGCGGAGTCGATGCAGTTGTCTTTTGATTCAAAAGGGAATAGTGTCCCTACTATTCTCTTGCTGATGCAGGAGAGATTATACTCACAAGGAGGATTGAAGGCAGAAGGAATATTCCGTATAAACCCAGAGAACAGCAAAGAGGAGTTTGTGAGAAACCAGTTGAACAGAGGCATTGTGCCTGATGACATTGACGTCCATTGCTTGGCAGGCCTAATCAAAGCCTGGTTTCGAGAACTTCCTTCAGGAGTGCTAGATGGACTATCTCCTGAAGAAGTTCTTCAGTGCAACACAGAAGAGGAATCCTTTGAGCTTGTGAAGCAGTTAAAGCCAACCGAGGCTGCATTACTCAATTGGGCTATCGGTCTGATGGCTGATGTTGTTGAGGAAGAAGAATTCAACAAAATGAATGCTAGAAATATTGCTATGGTTTTTGCTCCAAACATGACTCAGATGTCTGATCCACTAACGGCTTTGATGCATGCGGTTCAAGTAATGAATTTGCTTAAGACCCTGATTATGAAAACACTAAGGGAACGTGAAGAGACTGATGAAACTGGAGGATATTCACCCATGTCATCTTGTTTATCTGATCATCAATCAGACGAAGACTTGTATAGTCATCCAGACATGGATACCAATGGAGATTTGTATAGTCATCCAGACATGGATACCAACTCTGAATTGAGAGGACCAACATCAGATTATGATGAAAATGCCCACTATAGCAACAGCAGTGAAGATGAAGATGAAGTTCGGCCACTCAGTGACATAGAAGAATGCTTCTTAAGACGATTGGATGAGAAGAAAACAGCCCCAAATAGCATGGCAATGGTGAATAAACCAACCAGTAAGTCACTTGGTGAGTATCCGAGTCCTCAAAGTTGCTCGAGCTTTAACATGGAATCTGGCACATCTTCTGTTGATAGCAAAAATGGGAATTCTGGCTTCTGTGCAAGTTATGGGGAAGACTCTGGAAAATGCACTGATATGGAGAGCCCTTCAGTATCATGCTCAACCTTGAAGGAAATGGATATGGAGGACAGAGTAATGGAGTCTGTTTCAACTAGGCCATTGTTTGCATCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

527

Amino Acids

58.36

Weight (kDa)

4.65

Isoelectric Point (pI)

52.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 181 - 315 7.3e-20 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 686, 946
AatII GACGTC 1 cut(s) 690
AccB7I CCANNNNNTGG 1 cut(s) 697
AccII CGCG 2 cut(s) 281, 309
AciI CCGC 7 cut(s) 201, 279, 281, 307, 336, 497, 977
AclWI GGATC 1 cut(s) 947
AcoI YGGCCR 2 cut(s) 276, 1250
AcsI RAATTY 3 cut(s) 887, 991, 1432
AcuI CTGAAG 4 cut(s) 720, 758, 783, 1479
AcyI GRCGYC 1 cut(s) 687
AdeI CACNNNGTG 2 cut(s) 1259, 1355
AfiI CCNNNNNNNGG 3 cut(s) 285, 457, 697
AflII CTTAAG 2 cut(s) 998, 1279
AgeI ACCGGT 1 cut(s) 402
AjnI CCWGG 1 cut(s) 716
AjuI GAANNNNNNNTTGG 2 cut(s) 73, 105
AluBI AGCT 5 cut(s) 126, 231, 253, 805, 1386
AluI AGCT 5 cut(s) 126, 231, 253, 805, 1386
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 2 cut(s) 496, 1031
AlwI GGATC 1 cut(s) 947
Ama87I CYCGRG 2 cut(s) 451, 1381
AoxI GGCC 6 cut(s) 276, 300, 362, 703, 1250, 1562
ApeKI GCWGC 1 cut(s) 833
ApoI RAATTY 3 cut(s) 887, 991, 1432
AsiGI ACCGGT 1 cut(s) 402
Asp700I GAANNNNTTC 2 cut(s) 768, 771
AspS9I GGNCC 2 cut(s) 363, 1184
AsuC2I CCSGG 2 cut(s) 452, 453
AsuHPI GGTGA 6 cut(s) 202, 295, 340, 1053, 1339, 1367
AvaI CYCGRG 2 cut(s) 451, 1381
AvaII GGWCC 1 cut(s) 1184
BanII GRGCYC 3 cut(s) 183, 233, 1493
BbsI GAAGAC 3 cut(s) 416, 1107, 1467
Bbv12I GWGCWC 1 cut(s) 233
BbvI GCAGC 1 cut(s) 820
BccI CCATC 2 cut(s) 743, 856
BceAI ACGGC 2 cut(s) 323, 978
BciT130I CCWGG 1 cut(s) 718
BciVI GTATCC 3 cut(s) 1120, 1156, 1371
BclI TGATCA 1 cut(s) 1084
BcnI CCSGG 2 cut(s) 452, 453
BcoDI GTCTC 2 cut(s) 496, 1031
BfaI CTAG 4 cut(s) 471, 746, 906, 1560
BfmI CTRYAG 1 cut(s) 1216
BfrI CTTAAG 2 cut(s) 998, 1279
BfuI GTATCC 3 cut(s) 1120, 1156, 1371
BisI GCNGC 3 cut(s) 279, 307, 834
BlsI GCNGC 3 cut(s) 280, 308, 835
Bme1390I CCNGG 3 cut(s) 452, 453, 718
Bme18I GGWCC 1 cut(s) 1184
BmeT110I CYCGRG 2 cut(s) 451, 1381
BmgT120I GGNCC 2 cut(s) 363, 1184
BmiI GGNNCC 1 cut(s) 462
BmrFI CCNGG 3 cut(s) 452, 453, 718
BmsI GCATC 3 cut(s) 495, 549, 960
BoxI GACNNNNGTC 1 cut(s) 389
BpiI GAAGAC 3 cut(s) 416, 1107, 1467
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 1 cut(s) 1071
BpuMI CCSGG 2 cut(s) 452, 453
BsaHI GRCGYC 1 cut(s) 687
BsaI GGTCTC 1 cut(s) 496
BsaJI CCNNGG 4 cut(s) 279, 294, 451, 828
BsaWI WCCGGW 2 cut(s) 402, 427
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 3 cut(s) 285, 457, 697
Bse118I RCCGGY 1 cut(s) 402
Bse1I ACTGG 3 cut(s) 652, 1054, 1341
Bse3DI GCAATG 2 cut(s) 691, 1329
BseBI CCWGG 1 cut(s) 718
BseDI CCNNGG 4 cut(s) 279, 294, 451, 828
BseGI GGATG 3 cut(s) 1114, 1150, 1297
BseLI CCNNNNNNNGG 3 cut(s) 285, 457, 697
BseMI GCAATG 2 cut(s) 691, 1329
BseMII CTCAG 3 cut(s) 507, 956, 1270
BseNI ACTGG 3 cut(s) 652, 1054, 1341
BseRI GAGGAG 3 cut(s) 61, 338, 650
BseXI GCAGC 1 cut(s) 820
BseYI CCCAGC 1 cut(s) 131
Bsh1236I CGCG 2 cut(s) 281, 309
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 6 cut(s) 278, 302, 364, 705, 1252, 1564
BshTI ACCGGT 1 cut(s) 402
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 2 cut(s) 451, 1381
BsiSI CCGG 4 cut(s) 376, 403, 428, 452
BslFI GGGAC 2 cut(s) 434, 524
BslI CCNNNNNNNGG 3 cut(s) 285, 457, 697
BsmAI GTCTC 2 cut(s) 496, 1031
BsmFI GGGAC 2 cut(s) 434, 524
BsmI GAATGC 2 cut(s) 907, 1277
BsnI GGCC 6 cut(s) 278, 302, 364, 705, 1252, 1564
Bso31I GGTCTC 1 cut(s) 496
BsoBI CYCGRG 2 cut(s) 451, 1381
Bsp1286I GDGCHC 3 cut(s) 183, 233, 1493
Bsp143I GATC 4 cut(s) 330, 354, 952, 1084
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 7 cut(s) 201, 279, 281, 307, 336, 497, 977
BspANI GGCC 6 cut(s) 278, 302, 364, 705, 1252, 1564
BspCNI CTCAG 3 cut(s) 506, 955, 1269
BspFNI CGCG 2 cut(s) 281, 309
BspLI GGNNCC 1 cut(s) 462
BspPI GGATC 1 cut(s) 947
BspQI GCTCTTC 2 cut(s) 221, 234
BspTI CTTAAG 2 cut(s) 998, 1279
BspTNI GGTCTC 1 cut(s) 496
BsrDI GCAATG 2 cut(s) 691, 1329
BsrFI RCCGGY 1 cut(s) 402
BsrI ACTGG 3 cut(s) 652, 1054, 1341
BssAI RCCGGY 1 cut(s) 402
BssECI CCNNGG 4 cut(s) 279, 294, 451, 828
BssMI GATC 4 cut(s) 330, 354, 952, 1084
BssNI GRCGYC 1 cut(s) 687
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 718
Bst6I CTCTTC 5 cut(s) 112, 221, 234, 783, 1029
BstACI GRCGYC 1 cut(s) 687
BstAFI CTTAAG 2 cut(s) 998, 1279
BstC8I GCNNGC 3 cut(s) 304, 703, 975
BstDEI CTNAG 4 cut(s) 493, 942, 1022, 1256
BstDSI CCRYGG 2 cut(s) 279, 294
BstF5I GGATG 3 cut(s) 1114, 1150, 1297
BstFNI CGCG 2 cut(s) 281, 309
BstKTI GATC 4 cut(s) 333, 357, 955, 1087
BstMAI GTCTC 2 cut(s) 496, 1031
BstMBI GATC 4 cut(s) 330, 354, 952, 1084
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 5 cut(s) 250, 385, 702, 811, 820
BstNI CCWGG 1 cut(s) 718
BstNSI RCATGY 3 cut(s) 383, 392, 977
BstPAI GACNNNNGTC 1 cut(s) 389
BstSCI CCNGG 3 cut(s) 450, 451, 716
BstSFI CTRYAG 1 cut(s) 1216
BstUI CGCG 2 cut(s) 281, 309
BstV1I GCAGC 1 cut(s) 820
BstV2I GAAGAC 3 cut(s) 416, 1107, 1467
BsuI GTATCC 3 cut(s) 1120, 1156, 1371
BsuRI GGCC 6 cut(s) 278, 302, 364, 705, 1252, 1564
BtgI CCRYGG 2 cut(s) 279, 294
BtsCI GGATG 3 cut(s) 1114, 1150, 1297
BtsI GCAGTG 1 cut(s) 1234
BtsIMutI CAGTG 4 cut(s) 782, 1234, 1264, 1476
Cac8I GCNNGC 3 cut(s) 304, 703, 975
Cfr10I RCCGGY 1 cut(s) 402
Cfr13I GGNCC 2 cut(s) 363, 1184
Cfr42I CCGCGG 1 cut(s) 282
Cfr9I CCCGGG 1 cut(s) 451
CseI GACGC 1 cut(s) 394
CspAI ACCGGT 1 cut(s) 402
DdeI CTNAG 4 cut(s) 493, 942, 1022, 1256
DpnI GATC 4 cut(s) 332, 356, 954, 1086
DpnII GATC 4 cut(s) 330, 354, 952, 1084
DraIII CACNNNGTG 2 cut(s) 1259, 1355
DrdI GACNNNNNNGTC 2 cut(s) 686, 946
DseDI GACNNNNNNGTC 2 cut(s) 686, 946
EaeI YGGCCR 2 cut(s) 276, 1250
Eam1104I CTCTTC 5 cut(s) 112, 221, 234, 783, 1029
EarI CTCTTC 5 cut(s) 112, 221, 234, 783, 1029
EciI GGCGGA 1 cut(s) 325
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 294
Eco147I AGGCCT 1 cut(s) 705
Eco24I GRGCYC 3 cut(s) 183, 233, 1493
Eco31I GGTCTC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 1184
Eco53kI GAGCTC 1 cut(s) 231
Eco57I CTGAAG 4 cut(s) 720, 758, 783, 1479
Eco88I CYCGRG 2 cut(s) 451, 1381
EcoICRI GAGCTC 1 cut(s) 231
EcoRI GAATTC 2 cut(s) 887, 1432
EcoRII CCWGG 1 cut(s) 716
EcoT14I CCWWGG 1 cut(s) 294
EcoT22I ATGCAT 1 cut(s) 975
EcoT38I GRGCYC 3 cut(s) 183, 233, 1493
ErhI CCWWGG 1 cut(s) 294
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 2 cut(s) 434, 524
FbaI TGATCA 1 cut(s) 1084
Fnu4HI GCNGC 3 cut(s) 279, 307, 834
FokI GGATG 3 cut(s) 1101, 1137, 1304
FriOI GRGCYC 3 cut(s) 183, 233, 1493
Fsp4HI GCNGC 3 cut(s) 279, 307, 834
FspBI CTAG 4 cut(s) 471, 746, 906, 1560
GluI GCNGC 3 cut(s) 279, 307, 834
GsaI CCCAGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 1071
HaeIII GGCC 6 cut(s) 278, 302, 364, 705, 1252, 1564
HapII CCGG 4 cut(s) 376, 403, 428, 452
HgaI GACGC 1 cut(s) 394
Hin1I GRCGYC 1 cut(s) 687
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 9 cut(s) 155, 500, 521, 794, 940, 1366, 1397, 1463, 1547
HpaII CCGG 4 cut(s) 376, 403, 428, 452
HphI GGTGA 6 cut(s) 202, 295, 340, 1053, 1339, 1367
Hpy188III TCNNGA 6 cut(s) 725, 738, 761, 1118, 1154, 1467
HpyAV CCTTC 5 cut(s) 586, 593, 744, 1503, 1510
HpyCH4IV ACGT 3 cut(s) 395, 687, 1030
HpyF10VI GCNNNNNNNGC 5 cut(s) 250, 385, 702, 811, 820
HpyF3I CTNAG 4 cut(s) 493, 942, 1022, 1256
HpySE526I ACGT 3 cut(s) 395, 687, 1030
Hsp92I GRCGYC 1 cut(s) 687
Ksp22I TGATCA 1 cut(s) 1084
KspI CCGCGG 1 cut(s) 282
Kzo9I GATC 4 cut(s) 330, 354, 952, 1084
LguI GCTCTTC 2 cut(s) 221, 234
LmnI GCTCC 2 cut(s) 313, 934
Lsp1109I GCAGC 1 cut(s) 820
LweI GCATC 3 cut(s) 495, 549, 960
MaeI CTAG 4 cut(s) 471, 746, 906, 1560
MaeII ACGT 3 cut(s) 395, 687, 1030
MaeIII GTNAC 5 cut(s) 190, 382, 391, 1259, 1347
MalI GATC 4 cut(s) 332, 356, 954, 1086
MboI GATC 4 cut(s) 330, 354, 952, 1084
MfeI CAATTG 1 cut(s) 844
MhlI GDGCHC 3 cut(s) 183, 233, 1493
MluCI AATT 6 cut(s) 142, 844, 887, 991, 1175, 1432
MlyI GAGTC 5 cut(s) 509, 934, 1375, 1457, 1556
Mph1103I ATGCAT 1 cut(s) 975
MroXI GAANNNNTTC 2 cut(s) 768, 771
MseI TTAA 6 cut(s) 45, 818, 999, 1280, 1389, 1582
MslI CAYNNNNRTG 3 cut(s) 170, 1322, 1481
MspA1I CMGCKG 2 cut(s) 281, 497
MspCI CTTAAG 2 cut(s) 998, 1279
MspI CCGG 4 cut(s) 376, 403, 428, 452
MspR9I CCNGG 3 cut(s) 452, 453, 718
MunI CAATTG 1 cut(s) 844
Mva1269I GAATGC 2 cut(s) 907, 1277
MvaI CCWGG 1 cut(s) 718
MvnI CGCG 2 cut(s) 281, 309
MwoI GCNNNNNNNGC 5 cut(s) 250, 385, 702, 811, 820
NciI CCSGG 2 cut(s) 452, 453
NcoI CCATGG 1 cut(s) 294
NdeII GATC 4 cut(s) 330, 354, 952, 1084
NlaIV GGNNCC 1 cut(s) 462
NmuCI GTSAC 5 cut(s) 190, 382, 391, 1259, 1347
NsiI ATGCAT 1 cut(s) 975
NspI RCATGY 3 cut(s) 383, 392, 977
PaeI GCATGC 1 cut(s) 977
PaeR7I CTCGAG 1 cut(s) 1381
PceI AGGCCT 1 cut(s) 705
PciSI GCTCTTC 2 cut(s) 221, 234
PcsI WCGNNNNNNNCGW 1 cut(s) 392
PctI GAATGC 2 cut(s) 907, 1277
PdmI GAANNNNTTC 2 cut(s) 768, 771
PfeI GAWTC 4 cut(s) 155, 521, 794, 1397
PflMI CCANNNNNTGG 1 cut(s) 697
PinAI ACCGGT 1 cut(s) 402
PkrI GCNGC 3 cut(s) 280, 308, 835
PleI GAGTC 5 cut(s) 508, 934, 1374, 1457, 1555
PpsI GAGTC 5 cut(s) 508, 934, 1374, 1457, 1555
PshAI GACNNNNGTC 1 cut(s) 389
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 716
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 1 cut(s) 716
PspN4I GGNNCC 1 cut(s) 462
PspPI GGNCC 2 cut(s) 363, 1184
PspXI VCTCGAGB 1 cut(s) 1381
RseI CAYNNNNRTG 3 cut(s) 170, 1322, 1481
SacI GAGCTC 1 cut(s) 233
SacII CCGCGG 1 cut(s) 282
SapI GCTCTTC 2 cut(s) 221, 234
SaqAI TTAA 6 cut(s) 45, 818, 999, 1280, 1389, 1582
SatI GCNGC 3 cut(s) 279, 307, 834
Sau3AI GATC 4 cut(s) 330, 354, 952, 1084
Sau96I GGNCC 2 cut(s) 363, 1184
SchI GAGTC 5 cut(s) 509, 934, 1375, 1457, 1556
ScrFI CCNGG 3 cut(s) 452, 453, 718
SduI GDGCHC 3 cut(s) 183, 233, 1493
SfaNI GCATC 3 cut(s) 495, 549, 960
SfcI CTRYAG 1 cut(s) 1216
Sfr274I CTCGAG 1 cut(s) 1381
Sfr303I CCGCGG 1 cut(s) 282
SgrBI CCGCGG 1 cut(s) 282
SinI GGWCC 1 cut(s) 1184
SlaI CTCGAG 1 cut(s) 1381
SmaI CCCGGG 1 cut(s) 453
SmiMI CAYNNNNRTG 3 cut(s) 170, 1322, 1481
SmlI CTYRAG 3 cut(s) 998, 1279, 1381
SmoI CTYRAG 3 cut(s) 998, 1279, 1381
SphI GCATGC 1 cut(s) 977
Sse9I AATT 6 cut(s) 142, 844, 887, 991, 1175, 1432
SseBI AGGCCT 1 cut(s) 705
SsiI CCGC 7 cut(s) 201, 279, 281, 307, 336, 497, 977
SspI AATATT 2 cut(s) 606, 913
SspMI CTAG 4 cut(s) 471, 746, 906, 1560
SstI GAGCTC 1 cut(s) 233
StuI AGGCCT 1 cut(s) 705
StyD4I CCNGG 3 cut(s) 450, 451, 716
StyI CCWWGG 1 cut(s) 294
TaiI ACGT 3 cut(s) 398, 690, 1033
TaqI TCGA 6 cut(s) 399, 438, 444, 503, 724, 1382
TaqII GACCGA 1 cut(s) 845
TasI AATT 6 cut(s) 142, 844, 887, 991, 1175, 1432
TauI GCSGC 2 cut(s) 281, 309
TfiI GAWTC 4 cut(s) 155, 521, 794, 1397
Tru1I TTAA 6 cut(s) 45, 818, 999, 1280, 1389, 1582
Tru9I TTAA 6 cut(s) 45, 818, 999, 1280, 1389, 1582
TscAI CASTG 4 cut(s) 782, 1234, 1264, 1483
TseFI GTSAC 5 cut(s) 190, 382, 391, 1259, 1347
TseI GCWGC 1 cut(s) 833
Tsp45I GTSAC 5 cut(s) 190, 382, 391, 1259, 1347
TspDTI ATGAA 7 cut(s) 914, 1004, 1028, 1059, 1218, 1251, 1257
TspGWI ACGGA 1 cut(s) 599
TspMI CCCGGG 1 cut(s) 451
TspRI CASTG 4 cut(s) 782, 1234, 1264, 1483
Van91I CCANNNNNTGG 1 cut(s) 697
Vha464I CTTAAG 2 cut(s) 998, 1279
VpaK11BI GGWCC 1 cut(s) 1184
XapI RAATTY 3 cut(s) 887, 991, 1432
XceI RCATGY 3 cut(s) 383, 392, 977
XhoI CTCGAG 1 cut(s) 1381
XmaI CCCGGG 1 cut(s) 451
XmnI GAANNNNTTC 2 cut(s) 768, 771
XspI CTAG 4 cut(s) 471, 746, 906, 1560
ZraI GACGTC 1 cut(s) 688
Zsp2I ATGCAT 1 cut(s) 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.