MD08G1125900.v1.1

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006256
Physical Location & Seq
Reverse (-)
11825813 .. 11836534
10722 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1125900.v1.1.491

Sequence Viewer

Length: 3534 bp
ATGGCGATGATACATTTTCAACGGACCTTTTCACTGTCCACCGCCCCCGCTTCCTCCTTGATTTTCTCAAACAACTTAGCTTCCTCCGCCCTGCGTCTCCGCCGCTCCATGGCCAAGATGCAGTGGAGGGCGTTTGAGGCTTATCCATGTGCTTGCTTACCATCTTCGCTGAATTTGACAGCAAATGTATACAGAAATTGTTCTCAGCTTAGGTTAAAGAAATCCGGAGTTTCTTCCTTGATCACGGCCAACCATTCGAAGTTTGGGCACAATTTTCATTCTTTCAGAAGAAGCGGTGATGGGTCTGAACATAATTTTACCATTCATTCTAATTTAAAACATGGTTCGCTTAATTCTTTAGGGCCTGCTGGTATTATCAGTTTTGTCTCTTTTAAGAATAGTTGCAGATTAGGTTTAACTAATCATAGCGCGGGAGCTTTTCCCAAGAGGTTTTTCTCTCAAGTTCCATCTGTTATATACAAAGGAGAGAGTGTGGGTGTCCAAGATGGCAGGAATAGTGGAAATACATTTTTTTTCAGAAAGCTTAACAAACGCAAGAAACGGGAAAAGGCATTAGCAGCTCAAAGCAAAACCAAAGCTACTGATTCTAGCATCTCCAAAGATGTTGATGTAGAAGCTTTAACCGACCAGCAAGTTGGAAGTGAGAAGGGGGCTGGAAAATTGGGATCTTTGGTTTCCAATTCTCCTATAAGTAATGATACCAAGGTCTCTAAAGTAAAAGGGAAAAAGAGGTCAAAAAGCAAGAAAACTAAAGAACAAATTTCTTCCACAAATCCTCCAGCAGAAGCGGCTACAACACACAGTTCTAAGGTTACTTCTCAACCGAAGAAATCTGGTAGTACCAAAAGAGGCGAAAAGTCCGCGGAGGTTTCAGAGTTGAACCTCGGTGCGAAAACTCCCGTAGAAGTTGCTGATGGTTCTTCAACAAAACCCCAGCCAAAGAAGAAAGGCGGGAACTCTACGGGAAAGGGAAAATTGGTAAATTCAGATACTAAGTCGCCACAGAAACTTTTACCACAAAAAATAGGCAAAGTGAAACCTCATGGAAAAATGACACTGAAGCCACTTTACCCTGCAACTGGGAAAACTGTCGTGGTGGTGGAGTCTGCTACAAAAGCGAAGGTTATTCAGGGTTATCTTGGTGATATGTTTGAAGTTCTTCCTAGCTATGGTCATGTAAGGGACTTGGCTGCTAGGTCAGGATCTGTAAGGCCTGATGATGAGTTCAGTATGGTGTGGGAAGTTCCATCTGCCGCCTGGACTCATCTGAAAACTATAAAGGTTGCACTAACAGGAGCAGAAACTCTTATTCTTGCATCAGATCCTGATCGTGAAGGAGAGGCTATTGCTTGGCATATTATTGAGATGCTGCAACAACAGGATGCTTTACGTGAAGATATTACTGTAGCCAGGGTTGTTTTTCATGAAATCACTGAGGCCTCTATCAAAAGCGCGCTGCAAACTCCACGAGAAATTGATGAGAACTTGGTCCATGCTTACCTTGCACGGCGTGCTCTTGATTATTTGATTGGATTTAACATTTCACCACTGTTATGGAGGAAACTACCAGGTTGCCAGTCTGCGGGGCGAGTCCAATCTGCTGCATTGGCTCTTCTGTGCGATAGAGAAATGGAAATTGATGAATTTAAACCGCAGGAATATTGGACTACTGCAGTTGAATTGAAGAAAAAAGAAAAGGGTTCATCAGCGAATGCCCCATCCTTCCCAGCACACTTGACCCATTATGATTCCAAAAAGTTAAATCAATTTTCTATCAGTTCTCATACCGAGGCTAAAGTAATTGAACAGCAGATTAATTCAGAAATTTTTCAAGTTGTCAATTCTAAGAAAAGCAAAATGCGGAAGAATCCTCCCACGCCGTATATAACATCCACACTTCAGCAGGATGCTGCAAACAAATTGCATTTCAATGCAGCATATACAATGAAGCTTGCACAAAAACTTTACGAGGGGGTTCAGTTATCTGATGGTAAAGCAGCGGGTTTGATCACTTACACCCGAACAGATGGACTACATATTTCTGATGAAGCCTGTAAGGATGTGCGCTCCTTGGTCATTGAAAGGTATGGACAGAATTTTGCATCAGAAACTGCACGCAAATTTTTTAAGAAAGTGAAGAATGCTCAAGAGGCCCATGAAGCAATTAGACCCACTGATGTTCGAAGATTGCCATCATTGCTTCATGGGATACTTGATGAAGATTCCCTGAAGTTGTACACTCTAATTTGGTGTCGGACAGTGGCATGTCAGATGGAGCCTGCTACAATTGAGCAGATACAACTCGATATTGGAAATGCTGGCGAGTCCATTGTTTTCCGCTCTTCGTGCTCAAGGGTTGAGTTCCCTGGATATCAGGCAGTCTTTGAGGATGTGGAAGCTCAAGCAGTCAAATATAAAGAACAGGAAGGGAAGAGTCATGATGAATCCTTTGTACTTCTTAATTCTTTGAAGCGCGGGGATCCATTATGTATTGGTAAAGTGGAACTCAAGGAGCACCATACTCAACCTCCTCCCCGCTACTCTGAGGCAGCACTGGTTAAGAAATTGGAGGAGCTTGGGATTGGAAGACCATCAACATATGCTAGCACTTTGAAGGTTTTACAGGATAGAAACTACGTGACAGTTAAAAGCCGTGTATTACATCCAGAATTTCGTGGTCGTATGGTGTCAGCATTTCTCTGCCACCATTTTTCCGAGGTCACAGATTACAGTTTTACTGCTGACATGGAGAATGAGCTCGATAACGTGTCTGCTGGCTTAACTGAATGGAAAGGCCTCCTAAAAGATTATTGGACCCGATTCAGCACTTATTGTGAGCGTACCAGCACTGTTCATATTCATCAGGTAGAGAAGATGATGGAAAAAAAATTTGGAGACTTCTTGTTTGCTTCTCTTCCCGACCAAAGCAGGACATGCCCATGTTGTATGGAAGGCAGTTTGATTTTCAAAGTGAGTCGGTTCGGTGCAGGCTATTTTATAGGTTGTGATCAACACCCGAAGTGCAAATACATTGCTAAGACATTATACGGTGAAGAAGAAGAAGAAGAAGATGGTCCAAAAAAGAACAGCTGCGTGGAGGAACCAAAACTGCTAGGTCTAAGTCCAGGCTCCAATGAAAAGGTTCTTCTGAAGAAAGGTCCTTACGGATGCTATGTACAACTTGGTGAAGACAGGAAGACTAATGCACCTAAACGAGCCAACGTATCCCATATAAAGGATGTGAATTCGATTACCCTTGAATATGCGCTGGAGTTGCTGCGATATCCTGTGACTTTGGGAGACCATCCAGCGGATGGACAACCAGTCATTTTAAAGGTGGCAAAGGTTGGGTTTGCAGTTAGACATCGGCGCACAATTGCTTCTGTTCCTAAGGATATGAAACCCACTGAAATCACCCTCGAGGGCGCATTGAAGCTTTTGTCTGGTAAGGATGTAAGAAAATGTGGTCGACCTACGAGAAAACAAATTGAAGAAGCCCAAGCAGCCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

1178

Amino Acids

130.45

Weight (kDa)

9.36

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Toprim PF01751 370 - 484 8.4e-17 Toprim domain
Topoisom_bac PF01131 499 - 941 4e-111 DNA topoisomerase
Zn_ribbon_Top1 PF01396 984 - 1020 6.6e-07 Topoisomerase DNA binding C4 zinc finger
Toprim_C_rpt PF13368 1049 - 1110 2.8e-10 Topoisomerase C-terminal repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 3440
AccB7I CCANNNNNTGG 1 cut(s) 3335
AccBSI CCGCTC 2 cut(s) 105, 2361
AccI GTMKAC 2 cut(s) 189, 3490
AccII CGCG 4 cut(s) 431, 884, 1475, 2496
AccIII TCCGGA 1 cut(s) 224
AclWI GGATC 5 cut(s) 694, 1231, 1337, 2495, 2508
AcoI YGGCCR 2 cut(s) 111, 246
AcuI CTGAAG 4 cut(s) 1100, 1904, 2270, 3191
AdeI CACNNNGTG 1 cut(s) 1532
AfaI GTAC 5 cut(s) 862, 2258, 2475, 2863, 3198
AfiI CCNNNNNNNGG 7 cut(s) 109, 1100, 1190, 1603, 3256, 3331, 3335
AflIII ACRYGT 1 cut(s) 2787
AhdI GACNNNNNGTC 1 cut(s) 3344
AjnI CCWGG 5 cut(s) 1277, 1430, 1588, 2386, 3145
AjuI GAANNNNNNNTTGG 4 cut(s) 437, 469, 2895, 2927
Alw21I GWGCWC 4 cut(s) 1537, 2372, 2538, 2781
Alw26I GTCTC 5 cut(s) 101, 391, 733, 2910, 3315
AlwI GGATC 5 cut(s) 694, 1231, 1337, 2495, 2508
AlwNI CAGNNNCTG 3 cut(s) 1226, 1346, 2132
Ama87I CYCGRG 1 cut(s) 3440
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 7 cut(s) 111, 246, 362, 1232, 1458, 2172, 2815
ArsI GACNNNNNNTTYG 2 cut(s) 2909, 2941
AseI ATTAAT 1 cut(s) 1836
Asp700I GAANNNNTTC 4 cut(s) 199, 1179, 1848, 3162
AspLEI GCGC 8 cut(s) 431, 1475, 1477, 2088, 2496, 3289, 3393, 3449
AspS9I GGNCC 7 cut(s) 24, 362, 1510, 2173, 2835, 3095, 3179
AsuHPI GGTGA 6 cut(s) 308, 1175, 1557, 3083, 3218, 3427
AsuII TTCGAA 2 cut(s) 257, 2203
AsuNHI GCTAGC 1 cut(s) 2624
AvaI CYCGRG 1 cut(s) 3440
AvaII GGWCC 5 cut(s) 24, 1510, 2835, 3095, 3179
AxyI CCTNAGG 1 cut(s) 3411
BaeGI GKGCMC 1 cut(s) 270
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BalI TGGCCA 1 cut(s) 113
BamHI GGATCC 1 cut(s) 2500
BanII GRGCYC 1 cut(s) 2781
BarI GAAGNNNNNNTAC 4 cut(s) 1073, 1105, 3032, 3064
BauI CACGAG 1 cut(s) 1488
BbsI GAAGAC 3 cut(s) 2614, 3216, 3224
Bbv12I GWGCWC 4 cut(s) 1537, 2372, 2538, 2781
BceAI ACGGC 4 cut(s) 261, 1544, 1885, 2658
BciT130I CCWGG 5 cut(s) 1279, 1432, 1590, 2388, 3147
BciVI GTATCC 2 cut(s) 2223, 3256
BclI TGATCA 3 cut(s) 240, 2028, 3028
BcoDI GTCTC 5 cut(s) 101, 391, 733, 2910, 3315
BfaI CTAG 5 cut(s) 609, 1185, 1215, 2625, 3134
BfmI CTRYAG 2 cut(s) 1425, 1692
BfuI GTATCC 2 cut(s) 2223, 3256
Bme1390I CCNGG 5 cut(s) 1279, 1432, 1590, 2388, 3147
Bme18I GGWCC 5 cut(s) 24, 1510, 2835, 3095, 3179
BmeRI GACNNNNNGTC 1 cut(s) 3344
BmeT110I CYCGRG 1 cut(s) 3440
BmgT120I GGNCC 7 cut(s) 24, 362, 1510, 2173, 2835, 3095, 3179
BmiI GGNNCC 5 cut(s) 2298, 2502, 2837, 3123, 3151
BmrFI CCNGG 5 cut(s) 1279, 1432, 1590, 2388, 3147
BmrI ACTGGG 1 cut(s) 1110
BmsI GCATC 8 cut(s) 108, 621, 1346, 1377, 1393, 1918, 2132, 3179
BmtI GCTAGC 1 cut(s) 2628
BmuI ACTGGG 1 cut(s) 1110
BpiI GAAGAC 3 cut(s) 2614, 3216, 3224
BpmI CTGGAG 2 cut(s) 783, 3311
Bpu10I CCTNAGC 1 cut(s) 209
Bpu14I TTCGAA 2 cut(s) 257, 2203
BpuEI CTTGAG 5 cut(s) 444, 2151, 2356, 2406, 2513
BsaAI YACGTR 2 cut(s) 1412, 2659
BsaBI GATNNNNATC 2 cut(s) 1347, 2212
BsaI GGTCTC 2 cut(s) 733, 3315
BsaJI CCNNGG 9 cut(s) 108, 723, 882, 904, 1431, 1809, 2091, 2386, 2736
BsaWI WCCGGW 1 cut(s) 224
BsaXI ACNNNNNCTCC 6 cut(s) 477, 507, 781, 811, 2533, 2563
Bsc4I CCNNNNNNNGG 7 cut(s) 109, 1100, 1190, 1603, 3256, 3331, 3335
Bse1I ACTGG 4 cut(s) 1105, 1597, 2580, 3344
Bse21I CCTNAGG 1 cut(s) 3411
Bse3DI GCAATG 2 cut(s) 2216, 3051
Bse8I GATNNNNATC 2 cut(s) 1347, 2212
BseAI TCCGGA 1 cut(s) 224
BseBI CCWGG 5 cut(s) 1279, 1432, 1590, 2388, 3147
BseDI CCNNGG 9 cut(s) 108, 723, 882, 904, 1431, 1809, 2091, 2386, 2736
BseJI GATNNNNATC 2 cut(s) 1347, 2212
BseLI CCNNNNNNNGG 7 cut(s) 109, 1100, 1190, 1603, 3256, 3331, 3335
BseMI GCAATG 2 cut(s) 2216, 3051
BseMII CTCAG 3 cut(s) 218, 1446, 2556
BseNI ACTGG 4 cut(s) 1105, 1597, 2580, 3344
BsePI GCGCGC 1 cut(s) 1473
BseRI GAGGAG 2 cut(s) 2541, 2606
BseSI GKGCMC 1 cut(s) 270
BseYI CCCAGC 2 cut(s) 954, 1747
BsgI GTGCAG 2 cut(s) 2118, 3027
Bsh1236I CGCG 4 cut(s) 431, 884, 1475, 2496
BshFI GGCC 7 cut(s) 113, 248, 364, 1234, 1460, 2174, 2817
BsiHKAI GWGCWC 4 cut(s) 1537, 2372, 2538, 2781
BsiHKCI CYCGRG 1 cut(s) 3440
BsiSI CCGG 1 cut(s) 225
BslFI GGGAC 1 cut(s) 1217
BslI CCNNNNNNNGG 7 cut(s) 109, 1100, 1190, 1603, 3256, 3331, 3335
BsmAI GTCTC 5 cut(s) 101, 391, 733, 2910, 3315
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 1217
BsmI GAATGC 2 cut(s) 1738, 2167
BsnI GGCC 7 cut(s) 113, 248, 364, 1234, 1460, 2174, 2817
Bso31I GGTCTC 2 cut(s) 733, 3315
BsoBI CYCGRG 1 cut(s) 3440
Bsp119I TTCGAA 2 cut(s) 257, 2203
Bsp1286I GDGCHC 5 cut(s) 270, 1537, 2372, 2538, 2781
Bsp13I TCCGGA 1 cut(s) 224
Bsp1407I TGTACA 2 cut(s) 2256, 3196
Bsp143I GATC 8 cut(s) 240, 686, 1223, 1342, 1348, 2028, 2500, 3028
Bsp19I CCATGG 1 cut(s) 108
BspANI GGCC 7 cut(s) 113, 248, 364, 1234, 1460, 2174, 2817
BspCNI CTCAG 3 cut(s) 217, 1447, 2557
BspEI TCCGGA 1 cut(s) 224
BspFNI CGCG 4 cut(s) 431, 884, 1475, 2496
BspHI TCATGA 2 cut(s) 1444, 2458
BspLI GGNNCC 5 cut(s) 2298, 2502, 2837, 3123, 3151
BspMAI CTGCAG 1 cut(s) 1696
BspOI GCTAGC 1 cut(s) 2628
BspPI GGATC 5 cut(s) 694, 1231, 1337, 2495, 2508
BspQI GCTCTTC 2 cut(s) 1638, 2368
BspT104I TTCGAA 2 cut(s) 257, 2203
BspTNI GGTCTC 2 cut(s) 733, 3315
BsrBI CCGCTC 2 cut(s) 105, 2361
BsrDI GCAATG 2 cut(s) 2216, 3051
BsrGI TGTACA 2 cut(s) 2256, 3196
BsrI ACTGG 4 cut(s) 1105, 1597, 2580, 3344
BssECI CCNNGG 9 cut(s) 108, 723, 882, 904, 1431, 1809, 2091, 2386, 2736
BssHII GCGCGC 1 cut(s) 1473
BssMI GATC 8 cut(s) 240, 686, 1223, 1342, 1348, 2028, 2500, 3028
BssNAI GTATAC 1 cut(s) 190
BssSI CACGAG 1 cut(s) 1488
BssT1I CCWWGG 3 cut(s) 108, 723, 2091
Bst1107I GTATAC 1 cut(s) 190
Bst2BI CACGAG 1 cut(s) 1488
Bst2UI CCWGG 5 cut(s) 1279, 1432, 1590, 2388, 3147
Bst6I CTCTTC 4 cut(s) 1638, 2368, 2447, 2940
BstAPI GCANNNNNTGC 2 cut(s) 1532, 2955
BstAUI TGTACA 2 cut(s) 2256, 3196
BstBAI YACGTR 2 cut(s) 1412, 2659
BstBI TTCGAA 2 cut(s) 257, 2203
BstDSI CCRYGG 2 cut(s) 108, 882
BstFNI CGCG 4 cut(s) 431, 884, 1475, 2496
BstHHI GCGC 8 cut(s) 431, 1475, 1477, 2088, 2496, 3289, 3393, 3449
BstKTI GATC 8 cut(s) 243, 689, 1226, 1345, 1351, 2031, 2503, 3031
BstMAI GTCTC 5 cut(s) 101, 391, 733, 2910, 3315
BstMBI GATC 8 cut(s) 240, 686, 1223, 1342, 1348, 2028, 2500, 3028
BstNI CCWGG 5 cut(s) 1279, 1432, 1590, 2388, 3147
BstNSI RCATGY 2 cut(s) 2289, 2958
BstSCI CCNGG 5 cut(s) 1277, 1430, 1588, 2386, 3145
BstSFI CTRYAG 2 cut(s) 1425, 1692
BstSLI GKGCMC 1 cut(s) 270
BstUI CGCG 4 cut(s) 431, 884, 1475, 2496
BstV2I GAAGAC 3 cut(s) 2614, 3216, 3224
BstX2I RGATCY 4 cut(s) 686, 1223, 1342, 2500
BstXI CCANNNNNNTGG 2 cut(s) 656, 1575
BstYI RGATCY 4 cut(s) 686, 1223, 1342, 2500
BstZ17I GTATAC 1 cut(s) 190
Bsu36I CCTNAGG 1 cut(s) 3411
BsuI GTATCC 2 cut(s) 2223, 3256
BsuRI GGCC 7 cut(s) 113, 248, 364, 1234, 1460, 2174, 2817
BtgI CCRYGG 2 cut(s) 108, 882
BtgZI GCGATG 1 cut(s) 20
BtsI GCAGTG 1 cut(s) 128
CaiI CAGNNNCTG 3 cut(s) 1226, 1346, 2132
CciI TCATGA 2 cut(s) 1444, 2458
CfoI GCGC 8 cut(s) 431, 1475, 1477, 2088, 2496, 3289, 3393, 3449
Cfr13I GGNCC 7 cut(s) 24, 362, 1510, 2173, 2835, 3095, 3179
Cfr42I CCGCGG 1 cut(s) 885
CseI GACGC 1 cut(s) 83
CsiI ACCWGGT 1 cut(s) 1588
Csp6I GTAC 5 cut(s) 861, 2257, 2474, 2862, 3197
CviQI GTAC 5 cut(s) 861, 2257, 2474, 2862, 3197
DpnI GATC 8 cut(s) 242, 688, 1225, 1344, 1350, 2030, 2502, 3030
DpnII GATC 8 cut(s) 240, 686, 1223, 1342, 1348, 2028, 2500, 3028
DraI TTTAAA 3 cut(s) 336, 1669, 3354
DraIII CACNNNGTG 1 cut(s) 1532
DriI GACNNNNNGTC 1 cut(s) 3344
EaeI YGGCCR 2 cut(s) 111, 246
Eam1104I CTCTTC 4 cut(s) 1638, 2368, 2447, 2940
Eam1105I GACNNNNNGTC 1 cut(s) 3344
EarI CTCTTC 4 cut(s) 1638, 2368, 2447, 2940
EciI GGCGGA 2 cut(s) 76, 89
Ecl136II GAGCTC 1 cut(s) 2779
Eco130I CCWWGG 3 cut(s) 108, 723, 2091
Eco147I AGGCCT 3 cut(s) 1234, 1460, 2817
Eco24I GRGCYC 1 cut(s) 2781
Eco31I GGTCTC 2 cut(s) 733, 3315
Eco32I GATATC 2 cut(s) 2393, 3305
Eco47I GGWCC 5 cut(s) 24, 1510, 2835, 3095, 3179
Eco53kI GAGCTC 1 cut(s) 2779
Eco57I CTGAAG 4 cut(s) 1100, 1904, 2270, 3191
Eco81I CCTNAGG 1 cut(s) 3411
Eco88I CYCGRG 1 cut(s) 3440
EcoICRI GAGCTC 1 cut(s) 2779
EcoO109I RGGNCCY 2 cut(s) 362, 3179
EcoRI GAATTC 1 cut(s) 3265
EcoRII CCWGG 5 cut(s) 1277, 1430, 1588, 2386, 3145
EcoRV GATATC 2 cut(s) 2393, 3305
EcoT14I CCWWGG 3 cut(s) 108, 723, 2091
EcoT38I GRGCYC 1 cut(s) 2781
ErhI CCWWGG 3 cut(s) 108, 723, 2091
Esp3I CGTCTC 1 cut(s) 101
FaqI GGGAC 1 cut(s) 1217
FauI CCCGC 7 cut(s) 55, 424, 965, 1597, 2014, 2489, 2564
FauNDI CATATG 1 cut(s) 2620
FbaI TGATCA 3 cut(s) 240, 2028, 3028
FblI GTMKAC 2 cut(s) 189, 3490
FriOI GRGCYC 1 cut(s) 2781
FspBI CTAG 5 cut(s) 609, 1185, 1215, 2625, 3134
GlaI GCGC 8 cut(s) 430, 1474, 1476, 2087, 2495, 3288, 3392, 3448
GsaI CCCAGC 2 cut(s) 958, 1751
GsuI CTGGAG 2 cut(s) 783, 3311
HaeIII GGCC 7 cut(s) 113, 248, 364, 1234, 1460, 2174, 2817
HapII CCGG 1 cut(s) 225
HgaI GACGC 1 cut(s) 83
HhaI GCGC 8 cut(s) 431, 1475, 1477, 2088, 2496, 3289, 3393, 3449
Hin6I GCGC 8 cut(s) 429, 1473, 1475, 2086, 2494, 3287, 3391, 3447
HinP1I GCGC 8 cut(s) 429, 1473, 1475, 2086, 2494, 3287, 3391, 3447
HincII GTYRAC 1 cut(s) 3491
HindII GTYRAC 1 cut(s) 3491
HindIII AAGCTT 4 cut(s) 542, 636, 1970, 3455
HpaII CCGG 1 cut(s) 225
HphI GGTGA 6 cut(s) 308, 1175, 1557, 3083, 3218, 3427
Hpy166II GTNNAC 4 cut(s) 39, 190, 2259, 3491
Hpy8I GTNNAC 4 cut(s) 39, 190, 2259, 3491
HpyAV CCTTC 7 cut(s) 661, 1135, 1349, 1753, 2441, 2629, 2966
HpyCH4IV ACGT 4 cut(s) 1411, 2658, 2787, 3243
HpySE526I ACGT 4 cut(s) 1411, 2658, 2787, 3243
HspAI GCGC 8 cut(s) 429, 1473, 1475, 2086, 2494, 3287, 3391, 3447
Kpn2I TCCGGA 1 cut(s) 224
Ksp22I TGATCA 3 cut(s) 240, 2028, 3028
KspI CCGCGG 1 cut(s) 885
Kzo9I GATC 8 cut(s) 240, 686, 1223, 1342, 1348, 2028, 2500, 3028
LguI GCTCTTC 2 cut(s) 1638, 2368
LmnI GCTCC 8 cut(s) 110, 434, 1316, 2093, 2296, 2533, 2593, 3155
LweI GCATC 8 cut(s) 108, 621, 1346, 1377, 1393, 1918, 2132, 3179
MabI ACCWGGT 1 cut(s) 1588
MaeI CTAG 5 cut(s) 609, 1185, 1215, 2625, 3134
MaeII ACGT 4 cut(s) 1411, 2658, 2787, 3243
MaeIII GTNAC 4 cut(s) 832, 2659, 2740, 3310
MalI GATC 8 cut(s) 242, 688, 1225, 1344, 1350, 2030, 2502, 3030
MbiI CCGCTC 2 cut(s) 105, 2361
MboI GATC 8 cut(s) 240, 686, 1223, 1342, 1348, 2028, 2500, 3028
MfeI CAATTG 2 cut(s) 2307, 3396
MflI RGATCY 4 cut(s) 686, 1223, 1342, 2500
MhlI GDGCHC 5 cut(s) 270, 1537, 2372, 2538, 2781
MlsI TGGCCA 1 cut(s) 113
MluNI TGGCCA 1 cut(s) 113
MlyI GAGTC 6 cut(s) 1133, 1276, 1620, 2354, 2464, 3004
MmeI TCCRAC 2 cut(s) 637, 2255
Mox20I TGGCCA 1 cut(s) 113
MroI TCCGGA 1 cut(s) 224
MroXI GAANNNNTTC 4 cut(s) 199, 1179, 1848, 3162
MscI TGGCCA 1 cut(s) 113
MslI CAYNNNNRTG 3 cut(s) 1573, 1950, 2959
Msp20I TGGCCA 1 cut(s) 113
MspA1I CMGCKG 4 cut(s) 884, 2021, 3111, 3332
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 5 cut(s) 1279, 1432, 1590, 2388, 3147
MunI CAATTG 2 cut(s) 2307, 3396
Mva1269I GAATGC 2 cut(s) 1738, 2167
MvaI CCWGG 5 cut(s) 1279, 1432, 1590, 2388, 3147
MvnI CGCG 4 cut(s) 431, 884, 1475, 2496
NcoI CCATGG 1 cut(s) 108
NdeI CATATG 1 cut(s) 2620
NdeII GATC 8 cut(s) 240, 686, 1223, 1342, 1348, 2028, 2500, 3028
NheI GCTAGC 1 cut(s) 2624
NlaIV GGNNCC 5 cut(s) 2298, 2502, 2837, 3123, 3151
NmuCI GTSAC 3 cut(s) 2659, 2740, 3310
NspI RCATGY 2 cut(s) 2289, 2958
NspV TTCGAA 2 cut(s) 257, 2203
PaeR7I CTCGAG 1 cut(s) 3440
PagI TCATGA 2 cut(s) 1444, 2458
PauI GCGCGC 1 cut(s) 1473
PceI AGGCCT 3 cut(s) 1234, 1460, 2817
PciSI GCTCTTC 2 cut(s) 1638, 2368
PctI GAATGC 2 cut(s) 1738, 2167
PdmI GAANNNNTTC 4 cut(s) 199, 1179, 1848, 3162
PfeI GAWTC 6 cut(s) 605, 1769, 1888, 2243, 2465, 2841
PflMI CCANNNNNTGG 1 cut(s) 3335
PleI GAGTC 6 cut(s) 1132, 1276, 1619, 2353, 2463, 3003
PpsI GAGTC 6 cut(s) 1132, 1276, 1619, 2353, 2463, 3003
Ppu21I YACGTR 2 cut(s) 1412, 2659
PpuMI RGGWCCY 1 cut(s) 3179
PshBI ATTAAT 1 cut(s) 1836
Psp124BI GAGCTC 1 cut(s) 2781
Psp5II RGGWCCY 1 cut(s) 3179
Psp6I CCWGG 5 cut(s) 1277, 1430, 1588, 2386, 3145
PspFI CCCAGC 2 cut(s) 954, 1747
PspGI CCWGG 5 cut(s) 1277, 1430, 1588, 2386, 3145
PspN4I GGNNCC 5 cut(s) 2298, 2502, 2837, 3123, 3151
PspPI GGNCC 7 cut(s) 24, 362, 1510, 2173, 2835, 3095, 3179
PspPPI RGGWCCY 1 cut(s) 3179
PspXI VCTCGAGB 1 cut(s) 3440
PstI CTGCAG 1 cut(s) 1696
PstNI CAGNNNCTG 3 cut(s) 1226, 1346, 2132
PsuI RGATCY 4 cut(s) 686, 1223, 1342, 2500
PteI GCGCGC 1 cut(s) 1473
PvuII CAGCTG 1 cut(s) 3111
RsaI GTAC 5 cut(s) 862, 2258, 2475, 2863, 3198
RsaNI GTAC 5 cut(s) 861, 2257, 2474, 2862, 3197
RseI CAYNNNNRTG 3 cut(s) 1573, 1950, 2959
SacI GAGCTC 1 cut(s) 2781
SacII CCGCGG 1 cut(s) 885
SalI GTCGAC 1 cut(s) 3489
SapI GCTCTTC 2 cut(s) 1638, 2368
Sau3AI GATC 8 cut(s) 240, 686, 1223, 1342, 1348, 2028, 2500, 3028
Sau96I GGNCC 7 cut(s) 24, 362, 1510, 2173, 2835, 3095, 3179
SchI GAGTC 6 cut(s) 1133, 1276, 1620, 2354, 2464, 3004
ScrFI CCNGG 5 cut(s) 1279, 1432, 1590, 2388, 3147
SduI GDGCHC 5 cut(s) 270, 1537, 2372, 2538, 2781
SexAI ACCWGGT 1 cut(s) 1588
SfaNI GCATC 8 cut(s) 108, 621, 1346, 1377, 1393, 1918, 2132, 3179
SfcI CTRYAG 2 cut(s) 1425, 1692
Sfr274I CTCGAG 1 cut(s) 3440
Sfr303I CCGCGG 1 cut(s) 885
SfuI TTCGAA 2 cut(s) 257, 2203
SgrBI CCGCGG 1 cut(s) 885
SinI GGWCC 5 cut(s) 24, 1510, 2835, 3095, 3179
SlaI CTCGAG 1 cut(s) 3440
SmiMI CAYNNNNRTG 3 cut(s) 1573, 1950, 2959
SmlI CTYRAG 6 cut(s) 459, 2166, 2371, 2421, 2528, 3440
SmoI CTYRAG 6 cut(s) 459, 2166, 2371, 2421, 2528, 3440
SseBI AGGCCT 3 cut(s) 1234, 1460, 2817
SspI AATATT 1 cut(s) 1682
SspMI CTAG 5 cut(s) 609, 1185, 1215, 2625, 3134
SstI GAGCTC 1 cut(s) 2781
StuI AGGCCT 3 cut(s) 1234, 1460, 2817
StyD4I CCNGG 5 cut(s) 1277, 1430, 1588, 2386, 3145
StyI CCWWGG 3 cut(s) 108, 723, 2091
TaiI ACGT 4 cut(s) 1414, 2661, 2790, 3246
TaqI TCGA 7 cut(s) 257, 2203, 2325, 2781, 3269, 3441, 3490
TatI WGTACW 3 cut(s) 2256, 2473, 3196
TauI GCSGC 3 cut(s) 105, 812, 1277
TfiI GAWTC 6 cut(s) 605, 1769, 1888, 2243, 2465, 2841
TseFI GTSAC 3 cut(s) 2659, 2740, 3310
Tsp45I GTSAC 3 cut(s) 2659, 2740, 3310
TspGWI ACGGA 2 cut(s) 37, 3201
Van91I CCANNNNNTGG 1 cut(s) 3335
VpaK11BI GGWCC 5 cut(s) 24, 1510, 2835, 3095, 3179
VspI ATTAAT 1 cut(s) 1836
XceI RCATGY 2 cut(s) 2289, 2958
XcmI CCANNNNNNNNNTGG 3 cut(s) 260, 1275, 3332
XhoI CTCGAG 1 cut(s) 3440
XmiI GTMKAC 2 cut(s) 189, 3490
XmnI GAANNNNTTC 4 cut(s) 199, 1179, 1848, 3162
XspI CTAG 5 cut(s) 609, 1185, 1215, 2625, 3134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.