Rh6BG450900

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0006256
Physical Location & Seq
Reverse (-)
66491296 .. 66503603
12308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG450900.1

Sequence Viewer

Length: 3501 bp
ATGATTCATTTCCACAGGGCCTTCTCATTGACAGCTCCTTCTTCCTCTTCATTCATGTTCTCATACCCTTCAACTTCTTCAGCTCTCAGACTCCGCCTCTCCATGGCCAAGCTGCAGTGTAGGGCGTTTGAGCATTATCCGGGCACTTGCTTGCCGTCTTCTCCAGTTTTGACATTGAATAATTACCGAAACTATTCCCAGCTTAGGTTTAGAAAATCCAGAGTTTCTTGCATGATCACGTCCTACAGTGCGAAAGTTGGGCCGACTTTTGCTCCTTTGAGAAGAAGTGGTGATGGATTTCAACGTGCTTTTAGCATTGATTCACACTTAAGACCGCGTGCCCTTAATGCTTTGGGGACTGCTAATGTTATGAGTCGTGGGTTTTTTGGAAATGGCTGTAGATCGAGTTTATTGGATTCTAGAGTTGGAGATTTTTCCAAGAGGTTTTTGTCTCAAGTTCCGGACGTGACAAGTAAAGTAGAGAATGTGGGCTCCCATGATGGCAGGAGGCCTGGAAATAGAAAATGGTTCAAAATAATGATTAGCAATCGCAAGAAATTGAAAAAGGCATTGGCTACTCAAAGTGAAACTGTTTCGAGCATCTCCAAAGATGCTGATGTAAAAGTATCAAATGAAAAGCAGGTTGGAAGTGAGAAGGAGGATGAGAAGCTTGGATCATCAACTTCAGATTCGCCTATAAGCAAAACTAAAGAGGTCTCTAAACCAAAAGCAAAAAAGAGGTCGAAAAGCAAGAAAACTAAAGAACAAGATTCTGCTGCTAATGCTATAGCAGAAGCTGCTTCGACACAAAGTTCTGAAAGTACTTCTCAAACAAAGAAATCTGGCAGGAACAAAAAAGGAGCAAAGTCTGCACAGGATTCAGAGACTCCAGTAGACCTTGCTGATAGTTCAACAACAAAACCCCAGAGAAACAAGAAAGGTGAGAAGGAAAAATCTATAAATTCAGCTGCCAAGTCGCCACAGAAACTTATACCACAAGAAATGGGAAAATTGAAACCTCCTGGAAAAATGACAACGAAGCAGCTGTACCCCGCAACTGGGAAAACTGTTGTGGTGGTGGAATCTGTTACAAAGGCGAAGGTTATTCAGGGTTATCTTGGTGATATGTTTGAAGTGCTGCCTAGCTATGGTCATGTGAGGGACTTGGCAGCTAGGTCAGGATCTGTGCGGCCCGATGATGATTTTAGTATGGTGTGGGAAGTTCCATCTGCTGCCTGGACTCATCTTAAGAGCATAAAGGTCGCCCTGAGTGGTGCAGAAAATCTTATCCTGGCATCAGATCCTGATCGTGAAGGAGAGGCTATTGCTTGGCATATTATTGAGATGCTGCAACAACAGGATGCTTTACGTAAAGATATTTATGTAGCAAGGGTTGTCTTTCATGAAATCACTGAGGCCTCCATCAAAAGTGCTCTGCAGGCTCCAAGAGAAATTGATCAAAATTTGGTCCATGCTTACCTTGCACGGCGTGCTCTTGATTATTTGATCGGATTTAACATTTCACCGTTGCTATGGAGGAAACTACCAAGTTGCCAGTCTGCCGGGCGAGTCCAATCTGCTGCACTAGCTCTTGTATGCGACAGGGAAATGGAAATCGACAGATTTAAACCACAAGAATATTGGACTATTGATATTGAATTGAACAAAAAAGAACCAGATTCATCAGTGAATGCCATATCCTTGCCAGCGCACTTGACCCATTTTGATTCCAAAAAGTTGGACCAATTTTCTATCAGTTCTCATACAGAGGCAAAAGATATTGAGCAGCAAATTAATTCAGAAGCTTTTAAAGTTATCAGCTCTAAGAGAAACAAAATGCGGAAGAACCCTCCTATGCCTTATATAACATCAACACTTCAGCAGGATGCCGCAAACAAATTACATTTCAGTGCAACATATACAATGAAGCTTGCACAGAAGCTTTATGAGGGGGTTCAATTATCTGATGGCAAGTCAACGGGTTTGATCACATACACCAGAACAGATGGACTACACATTTCGGATGAAGCAGTAGAAGATGTTTGCTCCTTGGTCATCGAAAGGTATGGACAGAACTTTGCATCAGCTACTCCACGCAAGTTTTTTAAGAAGGTGAAGAATGCTCAGGAGGCCCATGAAGCAATCAGACCTACTGATGTTCAAAGATTTCCATCGTTGCTCAGAGGGATACTTGATGAAGATTCATTGAAGTTGTACACTCTTATTTGGTGTCGGACAGTTGCATGCCAAATGGAGCTTGCTACAATTGAGCAGATACAACTTGATATTGGAAATGCTGGTGAGTCCATTGTTTTCCGATCTGCATGCTCAAGGGTTCAGTTCCTTGGATATCAGGCAGTTTTTCAGGACGTGGAAGCTGGAGCCGTCAAATATAAAGAAAATGAAGGGATCAACCGGGATGAAGCTTTTGCACTTCTTGATTCACTGAAGGTTGGCAGTCCAGTGCATCTTGGTGAAGTGGAACTCAAGCAACACCATACCCAACCTCCTCCACGCTACTCTGAGGCATCATTGGTTAAGAAATTAGAGGAGCTTGGGATTGGAAGACCTTCAACATATGCAAGCACTATAAAGGTTTTACAGGACAGAAACTATCTGACAGTGAAAAGCCGTGTTTTACATCCAGAGTTTCGTGGTCGTATGGTGTCAGCATTTCTCTGCCACCATTTTTCTGAGGTCACAGATTATAGCTTCACAGCTGATATGGAGACTGAGCTTGATAATGTCTCTGCTGGCTTAACTGAATGGAAAGGCCTCCTAAGAGATTACTGGACTCGATTCAGTGGTTATTGTGAGCGTACCAGTAATGTGACTATCCATCAGGTGGAGAAAATGATGGAAAAAAAATTTGGAGACTTCTTGTTTGCTACTCTCCCTGACCAAAGCAGGACATGTCCAAGCTGTAATGAAGGAACTTTGATCTTCAAAGTGAGTCGATTCGGTGCAGGCTATTTCATAGGTTGTGATACACACCCAAAGTGCAAGTACATTGCTAAGACATTGTATGGTAATGAAGAAGAAGACGACAATACTCCGAAGAACAACAGGGTGGAGGAACCAAAATTGTTAGGTTTATCTCCCGGTTCCAATGAAAAGGTTCTTTTGAAGACTGGTCCTTATGGATCGTATGTACAACTTGGTGAAGACAGGAAGGGATACTCACCTAAAAGAGCCTCTATAGCACATATCAAGGATGTGAACTCAGTAACCGTTGAAGTTGCTCTGGAGTTGATGCGATATCCTGTGACATTGGGGAACCATCCGAAGGATGGGCTACCAGTGATTTTAAAGATTGCAAGGTCCGGATATATAGTTAAACATCGACGCACAATGGCTTCTCTAACGAAGAATCAGGAACCTAATAAATTAACCCTGGAGGACGCATTGGAACTTTTGTCTGGGAAAAATACACGGAAAATTGGTCGACCCAAGGGAAAATCAAAGGTTGAAGAAAAAGGAGAAGCTATAGAAGCCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

1166

Amino Acids

130.04

Weight (kDa)

9.35

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Toprim PF01751 356 - 469 1.9e-16 Toprim domain
Topoisom_bac PF01131 485 - 926 2.8e-111 DNA topoisomerase
Zn_ribbon_Top1 PF01396 970 - 1006 6.7e-09 Topoisomerase DNA binding C4 zinc finger
Toprim_C_rpt PF13368 1034 - 1088 1e-08 Topoisomerase C-terminal repeat
Toprim_C_rpt PF13368 1094 - 1145 1.1e-07 Topoisomerase C-terminal repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 631
AccB7I CCANNNNNTGG 1 cut(s) 2845
AccI GTMKAC 2 cut(s) 894, 3445
AccII CGCG 1 cut(s) 337
AccIII TCCGGA 2 cut(s) 460, 3323
AciI CCGC 6 cut(s) 94, 335, 1053, 1189, 1840, 1890
AclWI GGATC 5 cut(s) 682, 1189, 1295, 2417, 3151
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 3 cut(s) 961, 1462, 2867
AcuI CTGAAG 4 cut(s) 63, 669, 1862, 2468
AdeI CACNNNGTG 1 cut(s) 1490
AfaI GTAC 6 cut(s) 823, 1049, 2216, 2821, 3008, 3153
AfiI CCNNNNNNNGG 8 cut(s) 103, 204, 1058, 1059, 1148, 2845, 3286, 3290
AflII CTTAAG 2 cut(s) 328, 1247
AflIII ACRYGT 1 cut(s) 2912
AjiI CACGTC 3 cut(s) 240, 466, 2371
AjnI CCWGG 5 cut(s) 511, 1021, 1235, 1290, 3393
AjuI GAANNNNNNNTTGG 6 cut(s) 554, 586, 627, 659, 2853, 2885
Alw21I GWGCWC 2 cut(s) 1435, 1495
Alw26I GTCTC 6 cut(s) 456, 721, 878, 2723, 2752, 2868
AlwI GGATC 5 cut(s) 682, 1189, 1295, 2417, 3151
AlwNI CAGNNNCTG 3 cut(s) 797, 1184, 1304
Aor13HI TCCGGA 2 cut(s) 460, 3323
AoxI GGCC 8 cut(s) 18, 105, 260, 509, 1190, 1416, 2130, 2773
ApoI RAATTY 3 cut(s) 961, 1462, 2867
ArsI GACNNNNNNTTYG 4 cut(s) 725, 757, 2867, 2899
AseI ATTAAT 1 cut(s) 1794
Asp700I GAANNNNTTC 3 cut(s) 193, 2569, 3117
AspLEI GCGC 1 cut(s) 1711
AspS9I GGNCC 8 cut(s) 18, 260, 1191, 1468, 1741, 2131, 3134, 3321
AsuC2I CCSGG 4 cut(s) 141, 1564, 2417, 3102
AsuHPI GGTGA 9 cut(s) 302, 953, 1133, 1515, 2125, 2312, 2486, 3173, 3174
AvaII GGWCC 4 cut(s) 1468, 1741, 3134, 3321
BaeGI GKGCMC 2 cut(s) 146, 343
BalI TGGCCA 1 cut(s) 107
BanII GRGCYC 1 cut(s) 494
BarI GAAGNNNNNNTAC 2 cut(s) 1031, 1063
BbsI GAAGAC 5 cut(s) 150, 2572, 3048, 3134, 3171
Bbv12I GWGCWC 2 cut(s) 1435, 1495
BceAI ACGGC 4 cut(s) 139, 1502, 2369, 2616
BcgI CGANNNNNNTGC 4 cut(s) 1243, 1277, 2307, 2341
BciT130I CCWGG 5 cut(s) 513, 1023, 1237, 1292, 3395
BciVI GTATCC 2 cut(s) 2181, 3170
BclI TGATCA 3 cut(s) 234, 1456, 1986
BcnI CCSGG 4 cut(s) 141, 1564, 2417, 3102
BcoDI GTCTC 6 cut(s) 456, 721, 878, 2723, 2752, 2868
BfaI CTAG 4 cut(s) 420, 1143, 1173, 1586
BfmI CTRYAG 7 cut(s) 113, 244, 397, 786, 1436, 3198, 3486
BfrI CTTAAG 2 cut(s) 328, 1247
BfuAI ACCTGC 1 cut(s) 631
BfuI GTATCC 2 cut(s) 2181, 3170
BmcAI AGTACT 1 cut(s) 823
Bme1390I CCNGG 9 cut(s) 141, 513, 1023, 1237, 1292, 1564, 2417, 3102, 3395
Bme18I GGWCC 4 cut(s) 1468, 1741, 3134, 3321
BmgBI CACGTC 3 cut(s) 240, 466, 2371
BmgT120I GGNCC 8 cut(s) 18, 260, 1191, 1468, 1741, 2131, 3134, 3321
BmiI GGNNCC 7 cut(s) 493, 1443, 2383, 3078, 3106, 3278, 3378
BmrFI CCNGG 9 cut(s) 141, 513, 1023, 1237, 1292, 1564, 2417, 3102, 3395
BmrI ACTGGG 1 cut(s) 1068
BmuI ACTGGG 1 cut(s) 1068
BpiI GAAGAC 5 cut(s) 150, 2572, 3048, 3134, 3171
BpmI CTGGAG 5 cut(s) 147, 873, 2400, 3266, 3416
Bpu10I CCTNAGC 2 cut(s) 203, 2124
BpuEI CTTGAG 3 cut(s) 438, 2314, 2471
BpuMI CCSGG 4 cut(s) 141, 1564, 2417, 3102
BsaAI YACGTR 1 cut(s) 1370
BsaBI GATNNNNATC 2 cut(s) 1305, 2170
BsaI GGTCTC 1 cut(s) 721
BsaJI CCNNGG 5 cut(s) 102, 2049, 2344, 3393, 3450
BsaWI WCCGGW 2 cut(s) 460, 3323
BsaXI ACNNNNNCTCC 4 cut(s) 256, 286, 2491, 2521
Bsc4I CCNNNNNNNGG 8 cut(s) 103, 204, 1058, 1059, 1148, 2845, 3286, 3290
Bse1I ACTGG 9 cut(s) 164, 890, 1063, 1555, 2462, 2795, 2823, 3136, 3299
Bse3DI GCAATG 1 cut(s) 3009
Bse8I GATNNNNATC 2 cut(s) 1305, 2170
BseAI TCCGGA 2 cut(s) 460, 3323
BseBI CCWGG 5 cut(s) 513, 1023, 1237, 1292, 3395
BseDI CCNNGG 5 cut(s) 102, 2049, 2344, 3393, 3450
BseGI GGATG 9 cut(s) 667, 1366, 1891, 2029, 2425, 2641, 3220, 3280, 3295
BseJI GATNNNNATC 2 cut(s) 1305, 2170
BseLI CCNNNNNNNGG 8 cut(s) 103, 204, 1058, 1059, 1148, 2845, 3286, 3290
BseMI GCAATG 1 cut(s) 3009
BseMII CTCAG 9 cut(s) 100, 1259, 1404, 2138, 2194, 2514, 2685, 2724, 3237
BseNI ACTGG 9 cut(s) 164, 890, 1063, 1555, 2462, 2795, 2823, 3136, 3299
BseRI GAGGAG 2 cut(s) 2499, 2564
BseSI GKGCMC 2 cut(s) 146, 343
BseYI CCCAGC 1 cut(s) 198
BsgI GTGCAG 4 cut(s) 855, 1296, 1566, 2985
Bsh1236I CGCG 1 cut(s) 337
BshFI GGCC 8 cut(s) 20, 107, 262, 511, 1192, 1418, 2132, 2775
BsiHKAI GWGCWC 2 cut(s) 1435, 1495
BsiSI CCGG 6 cut(s) 140, 461, 1563, 2416, 3102, 3324
BslFI GGGAC 2 cut(s) 370, 1175
BslI CCNNNNNNNGG 8 cut(s) 103, 204, 1058, 1059, 1148, 2845, 3286, 3290
BsmAI GTCTC 6 cut(s) 456, 721, 878, 2723, 2752, 2868
BsmFI GGGAC 2 cut(s) 370, 1175
BsmI GAATGC 2 cut(s) 1696, 2125
BsnI GGCC 8 cut(s) 20, 107, 262, 511, 1192, 1418, 2132, 2775
Bso31I GGTCTC 1 cut(s) 721
Bsp1286I GDGCHC 5 cut(s) 146, 343, 494, 1435, 1495
Bsp13I TCCGGA 2 cut(s) 460, 3323
Bsp1407I TGTACA 2 cut(s) 2214, 3151
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 6 cut(s) 94, 335, 1053, 1189, 1840, 1890
BspANI GGCC 8 cut(s) 20, 107, 262, 511, 1192, 1418, 2132, 2775
BspCNI CTCAG 9 cut(s) 99, 1260, 1405, 2137, 2193, 2515, 2686, 2725, 3236
BspEI TCCGGA 2 cut(s) 460, 3323
BspFNI CGCG 1 cut(s) 337
BspHI TCATGA 1 cut(s) 1402
BspLI GGNNCC 7 cut(s) 493, 1443, 2383, 3078, 3106, 3278, 3378
BspMAI CTGCAG 2 cut(s) 117, 1440
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 5 cut(s) 682, 1189, 1295, 2417, 3151
BspTI CTTAAG 2 cut(s) 328, 1247
BspTNI GGTCTC 1 cut(s) 721
BsrDI GCAATG 1 cut(s) 3009
BsrGI TGTACA 2 cut(s) 2214, 3151
BsrI ACTGG 9 cut(s) 164, 890, 1063, 1555, 2462, 2795, 2823, 3136, 3299
BssECI CCNNGG 5 cut(s) 102, 2049, 2344, 3393, 3450
BssT1I CCWWGG 4 cut(s) 102, 2049, 2344, 3450
Bst2UI CCWGG 5 cut(s) 513, 1023, 1237, 1292, 3395
Bst4CI ACNGT 7 cut(s) 248, 592, 1069, 1527, 2239, 2623, 3232
Bst6I CTCTTC 1 cut(s) 52
BstAFI CTTAAG 2 cut(s) 328, 1247
BstAPI GCANNNNNTGC 3 cut(s) 797, 869, 1490
BstAUI TGTACA 2 cut(s) 2214, 3151
BstBAI YACGTR 1 cut(s) 1370
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 9 cut(s) 667, 1366, 1891, 2029, 2425, 2641, 3220, 3280, 3295
BstFNI CGCG 1 cut(s) 337
BstHHI GCGC 1 cut(s) 1711
BstMAI GTCTC 6 cut(s) 456, 721, 878, 2723, 2752, 2868
BstNI CCWGG 5 cut(s) 513, 1023, 1237, 1292, 3395
BstNSI RCATGY 3 cut(s) 2247, 2328, 2916
BstSCI CCNGG 9 cut(s) 139, 511, 1021, 1235, 1290, 1562, 2415, 3100, 3393
BstSFI CTRYAG 7 cut(s) 113, 244, 397, 786, 1436, 3198, 3486
BstSLI GKGCMC 2 cut(s) 146, 343
BstSNI TACGTA 1 cut(s) 1370
BstUI CGCG 1 cut(s) 337
BstV2I GAAGAC 5 cut(s) 150, 2572, 3048, 3134, 3171
BstX2I RGATCY 2 cut(s) 1181, 1300
BstXI CCANNNNNNTGG 1 cut(s) 1738
BstYI RGATCY 2 cut(s) 1181, 1300
BsuI GTATCC 2 cut(s) 2181, 3170
BsuRI GGCC 8 cut(s) 20, 107, 262, 511, 1192, 1418, 2132, 2775
BtgI CCRYGG 1 cut(s) 102
BtrI CACGTC 3 cut(s) 240, 466, 2371
BtsCI GGATG 9 cut(s) 667, 1366, 1891, 2029, 2425, 2641, 3220, 3280, 3295
BtsI GCAGTG 1 cut(s) 122
BveI ACCTGC 1 cut(s) 631
CaiI CAGNNNCTG 3 cut(s) 797, 1184, 1304
CciI TCATGA 1 cut(s) 1402
CfoI GCGC 1 cut(s) 1711
Cfr13I GGNCC 8 cut(s) 18, 260, 1191, 1468, 1741, 2131, 3134, 3321
CseI GACGC 2 cut(s) 3354, 3410
Csp6I GTAC 6 cut(s) 822, 1048, 2215, 2820, 3007, 3152
CviQI GTAC 6 cut(s) 822, 1048, 2215, 2820, 3007, 3152
DraI TTTAAA 3 cut(s) 1627, 1810, 3309
DraIII CACNNNGTG 1 cut(s) 1490
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
EciI GGCGGA 1 cut(s) 83
Eco105I TACGTA 1 cut(s) 1370
Eco130I CCWWGG 4 cut(s) 102, 2049, 2344, 3450
Eco147I AGGCCT 3 cut(s) 511, 1418, 2775
Eco24I GRGCYC 1 cut(s) 494
Eco31I GGTCTC 1 cut(s) 721
Eco32I GATATC 2 cut(s) 2351, 3260
Eco47I GGWCC 4 cut(s) 1468, 1741, 3134, 3321
Eco57I CTGAAG 4 cut(s) 63, 669, 1862, 2468
EcoO109I RGGNCCY 1 cut(s) 18
EcoRII CCWGG 5 cut(s) 511, 1021, 1235, 1290, 3393
EcoRV GATATC 2 cut(s) 2351, 3260
EcoT14I CCWWGG 4 cut(s) 102, 2049, 2344, 3450
EcoT38I GRGCYC 1 cut(s) 494
ErhI CCWWGG 4 cut(s) 102, 2049, 2344, 3450
FaqI GGGAC 2 cut(s) 370, 1175
FauI CCCGC 1 cut(s) 1060
FauNDI CATATG 1 cut(s) 2578
FbaI TGATCA 3 cut(s) 234, 1456, 1986
FblI GTMKAC 2 cut(s) 894, 3445
FokI GGATG 9 cut(s) 674, 1373, 1898, 2036, 2432, 2628, 3227, 3267, 3302
FriOI GRGCYC 1 cut(s) 494
FspBI CTAG 4 cut(s) 420, 1143, 1173, 1586
GlaI GCGC 1 cut(s) 1710
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 5 cut(s) 147, 873, 2400, 3266, 3416
HaeIII GGCC 8 cut(s) 20, 107, 262, 511, 1192, 1418, 2132, 2775
HapII CCGG 6 cut(s) 140, 461, 1563, 2416, 3102, 3324
HgaI GACGC 2 cut(s) 3354, 3410
HhaI GCGC 1 cut(s) 1711
Hin6I GCGC 1 cut(s) 1709
HinP1I GCGC 1 cut(s) 1709
HincII GTYRAC 2 cut(s) 1977, 3446
HindII GTYRAC 2 cut(s) 1977, 3446
HindIII AAGCTT 5 cut(s) 668, 1803, 1928, 1940, 2424
HpaII CCGG 6 cut(s) 140, 461, 1563, 2416, 3102, 3324
HphI GGTGA 9 cut(s) 302, 953, 1133, 1515, 2125, 2312, 2486, 3173, 3174
Hpy166II GTNNAC 5 cut(s) 895, 1977, 2217, 3220, 3446
Hpy8I GTNNAC 5 cut(s) 895, 1977, 2217, 3220, 3446
Hpy99I CGWCG 1 cut(s) 3348
HpyCH4III ACNGT 7 cut(s) 248, 592, 1069, 1527, 2239, 2623, 3232
HpyCH4IV ACGT 5 cut(s) 239, 304, 465, 1369, 2370
HpySE526I ACGT 5 cut(s) 239, 304, 465, 1369, 2370
HspAI GCGC 1 cut(s) 1709
Kpn2I TCCGGA 2 cut(s) 460, 3323
Ksp22I TGATCA 3 cut(s) 234, 1456, 1986
LmnI GCTCC 9 cut(s) 40, 277, 497, 860, 1447, 2051, 2254, 2381, 2551
MaeI CTAG 4 cut(s) 420, 1143, 1173, 1586
MaeII ACGT 5 cut(s) 239, 304, 465, 1369, 2370
MaeIII GTNAC 6 cut(s) 466, 1087, 2698, 2830, 3226, 3265
MfeI CAATTG 1 cut(s) 2265
MflI RGATCY 2 cut(s) 1181, 1300
MhlI GDGCHC 5 cut(s) 146, 343, 494, 1435, 1495
MlsI TGGCCA 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 107
MlyI GAGTC 8 cut(s) 84, 382, 880, 1234, 1578, 2312, 2788, 2962
MmeI TCCRAC 4 cut(s) 406, 625, 1719, 2213
Mox20I TGGCCA 1 cut(s) 107
MroI TCCGGA 2 cut(s) 460, 3323
MroXI GAANNNNTTC 3 cut(s) 193, 2569, 3117
MscI TGGCCA 1 cut(s) 107
MslI CAYNNNNRTG 2 cut(s) 1908, 2472
Msp20I TGGCCA 1 cut(s) 107
MspA1I CMGCKG 3 cut(s) 968, 1045, 2720
MspCI CTTAAG 2 cut(s) 328, 1247
MspI CCGG 6 cut(s) 140, 461, 1563, 2416, 3102, 3324
MspR9I CCNGG 9 cut(s) 141, 513, 1023, 1237, 1292, 1564, 2417, 3102, 3395
MunI CAATTG 1 cut(s) 2265
Mva1269I GAATGC 2 cut(s) 1696, 2125
MvaI CCWGG 5 cut(s) 513, 1023, 1237, 1292, 3395
MvnI CGCG 1 cut(s) 337
NciI CCSGG 4 cut(s) 141, 1564, 2417, 3102
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 1 cut(s) 2578
NlaIV GGNNCC 7 cut(s) 493, 1443, 2383, 3078, 3106, 3278, 3378
NmuCI GTSAC 4 cut(s) 466, 2698, 2830, 3265
NspI RCATGY 3 cut(s) 2247, 2328, 2916
PaeI GCATGC 2 cut(s) 2247, 2328
PagI TCATGA 1 cut(s) 1402
PceI AGGCCT 3 cut(s) 511, 1418, 2775
PciI ACATGT 1 cut(s) 2912
PctI GAATGC 2 cut(s) 1696, 2125
PdmI GAANNNNTTC 3 cut(s) 193, 2569, 3117
PflMI CCANNNNNTGG 1 cut(s) 2845
PfoI TCCNGGA 1 cut(s) 1021
PleI GAGTC 8 cut(s) 84, 381, 880, 1234, 1577, 2311, 2788, 2961
PpsI GAGTC 8 cut(s) 84, 381, 880, 1234, 1577, 2311, 2788, 2961
Ppu21I YACGTR 1 cut(s) 1370
PscI ACATGT 1 cut(s) 2912
PshBI ATTAAT 1 cut(s) 1794
Psp6I CCWGG 5 cut(s) 511, 1021, 1235, 1290, 3393
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 5 cut(s) 511, 1021, 1235, 1290, 3393
PspN4I GGNNCC 7 cut(s) 493, 1443, 2383, 3078, 3106, 3278, 3378
PspPI GGNCC 8 cut(s) 18, 260, 1191, 1468, 1741, 2131, 3134, 3321
PstI CTGCAG 2 cut(s) 117, 1440
PstNI CAGNNNCTG 3 cut(s) 797, 1184, 1304
PsuI RGATCY 2 cut(s) 1181, 1300
PvuII CAGCTG 3 cut(s) 968, 1045, 2720
RsaI GTAC 6 cut(s) 823, 1049, 2216, 2821, 3008, 3153
RsaNI GTAC 6 cut(s) 822, 1048, 2215, 2820, 3007, 3152
RseI CAYNNNNRTG 2 cut(s) 1908, 2472
SalI GTCGAC 1 cut(s) 3444
Sau96I GGNCC 8 cut(s) 18, 260, 1191, 1468, 1741, 2131, 3134, 3321
ScaI AGTACT 1 cut(s) 823
SchI GAGTC 8 cut(s) 84, 382, 880, 1234, 1578, 2312, 2788, 2962
ScrFI CCNGG 9 cut(s) 141, 513, 1023, 1237, 1292, 1564, 2417, 3102, 3395
SduI GDGCHC 5 cut(s) 146, 343, 494, 1435, 1495
SfcI CTRYAG 7 cut(s) 113, 244, 397, 786, 1436, 3198, 3486
SinI GGWCC 4 cut(s) 1468, 1741, 3134, 3321
SmiMI CAYNNNNRTG 2 cut(s) 1908, 2472
SmlI CTYRAG 5 cut(s) 328, 453, 1247, 2329, 2486
SmoI CTYRAG 5 cut(s) 328, 453, 1247, 2329, 2486
SnaBI TACGTA 1 cut(s) 1370
SphI GCATGC 2 cut(s) 2247, 2328
SseBI AGGCCT 3 cut(s) 511, 1418, 2775
SsiI CCGC 6 cut(s) 94, 335, 1053, 1189, 1840, 1890
SspI AATATT 1 cut(s) 1640
SspMI CTAG 4 cut(s) 420, 1143, 1173, 1586
StuI AGGCCT 3 cut(s) 511, 1418, 2775
StyD4I CCNGG 9 cut(s) 139, 511, 1021, 1235, 1290, 1562, 2415, 3100, 3393
StyI CCWWGG 4 cut(s) 102, 2049, 2344, 3450
TaaI ACNGT 7 cut(s) 248, 592, 1069, 1527, 2239, 2623, 3232
TaiI ACGT 5 cut(s) 242, 307, 468, 1372, 2373
TatI WGTACW 4 cut(s) 821, 2214, 3006, 3151
TauI GCSGC 2 cut(s) 1192, 1892
TseFI GTSAC 4 cut(s) 466, 2698, 2830, 3265
Tsp45I GTSAC 4 cut(s) 466, 2698, 2830, 3265
TspGWI ACGGA 1 cut(s) 3448
Van91I CCANNNNNTGG 1 cut(s) 2845
Vha464I CTTAAG 2 cut(s) 328, 1247
VpaK11BI GGWCC 4 cut(s) 1468, 1741, 3134, 3321
VspI ATTAAT 1 cut(s) 1794
XapI RAATTY 3 cut(s) 961, 1462, 2867
XbaI TCTAGA 1 cut(s) 419
XceI RCATGY 3 cut(s) 2247, 2328, 2916
XcmI CCANNNNNNNNNTGG 3 cut(s) 1233, 1638, 3287
XmiI GTMKAC 2 cut(s) 894, 3445
XmnI GAANNNNTTC 3 cut(s) 193, 2569, 3117
XspI CTAG 4 cut(s) 420, 1143, 1173, 1586
ZrmI AGTACT 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.