RLG00000010906

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006256
Physical Location & Seq
Reverse (-)
5213656 .. 5226324
12669 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010906

Sequence Viewer

Length: 3465 bp
ATGGCCAAGCTGCAGTATAGGGCGTTTGAGCATTATCCCGGCACTTGCTTGCCGTCTTCTCCAGTTTTGACATTGAATAATTACCGAAACTATTCCCAGCTTAGGGTTAGAAAATCCAGAGTTTCTTGCATGATCACGTCCTATGGTGCGAAAGTTGGGCCGACTTTTGCTCCTTTGAGAAGAAGTGGTGATGGATTTCAACGTGCTTTTAGCATTGATTCACACTTAAGACCGCGTGCCCTTAATGCTTTGGGGACTGCTAATGTTATGAGTCGTGGGTTTTTTGGAAATGGCTGTAGATCGAGTTTATTGGATTCTAGAGTTGGAGATTTTTCTAAGAGGTTTTTGTCTCAAGTTCCTGATTCCACAGGTAAAGTAGAGAATGTGGGCTCCCAAGATGGCAGGAGGCCTGGAAATAGAAAATGGTTCAAAATAATGATTAGCAATCGCAAGAAATTGAAAAAGGCATTGGCTACTCAAAGTGAAACTGAAGCTACCGTTTCGAGCATCTCCAAAGATGCTGATGTAAAAGTTTCAGATGAAAAGCAGGTTGGAAGTGAGAAGAAGGATGAGAAGCTTGGATCACCAACTTCAGATTCGCCTATAAGCAAAACTAAAGAGGTCTCTAAACCAAAAGCGAAAAAGAGGTCGAAAAGCAAGAAAACTAAAGAACAAGATTCTGCTGCTAATGCTATAGCAGAAGCTGCTTCGACACAAAGTTCTGAAAGTACTTCTCAAACAAAGAAATCTGGCAGGAACAAAAAAGGAGCAAAGTCTGCACAGGATTCAGAGACTCCAGTAGACCTTGCTGATAGTTCAACAACAAAACCCCAGAGAAACAAGAAAGGTGAGAAGGAAAAATCTATAAATTCAGCTGCAAAGTCACCACAGAAACTTATACCACAAGAAATGGGAAAATTGAAACCTCCTGGAAAAATGACAACGAAGCAGCTGTACCCCGCAACTGGGAAAACTGTTGTGGTGGTGGAGTCTGTTACAAAGGCGAAGGTTATTCAGGGTTATCTTGGTGATATGTTTGAAGTGCTGCCTAGCTATGGTCATGTGAGGGACTTGGCAGCTAGGTCAGGATCTGTGCGGCCCGATGATGATTTTAGTATGGTGTGGGAAGTTCCATCTGCTGCCTGGACTCATCTTAAGAGCATAAAGGTCGCCCTAAGTGGTGCAGAAAATCTTATCCTTGCATCAGATCCTGATCGTGAAGGAGAGGCTATTGGTTGGCATATTATTGAGATGCTGCAACAACAGGATGCTTTACGTAAAGATATTTATGTAGCAAGGGTTGTCTTTCATGAAATCACTGAGGCCTCCATCAAAAGTGCTCTGCAGGCTCCAAGAGAAATTGATCAAAATTTGGTCCATGCTTACCTTGCACGGCGTGCTCTTGATTATTTGATTGGATTTAATATTTCACCGTTGCTATGGAGGAAACTGCCAAGTTGCCAGTCTGCCGGGCGAGTCCAATCTGCTGCACTAGCTCTTGTATGCGACAGGGAAATGGAAATCGACAGATTTAAACCACAAGAATATTGGACTATTGATATTGAATTGAACAAAAAAGAACCAGATTCATCAGTGAATGCCATATCCTTGCCAGCGCACTTGACCCATTTTGATTCCAAAAAGTTGGACCAATTTTCTATCAGTTCTCATACAGAGGCAAAAGATATTGAGCAGCAAATTAATTCAGAAGCTTTTAAAGTTATCAGCTCTAAGAGAAACAAAATGCGGAAGAACCCTCCTACGCCTTATATAACATCAACACTTCAGCAGGATGCCGCAAACAAATTACATTTCAGTGCAACATATACAATGAAGCTTGCACAGAAGCTTTATGAGGGGGTTCAATTATCTGATGGCAAGTCAACGGGTTTGATCACATACACCAGAACAGATGGACTACACATTTCGGATGAAGCAGTAGAAGGTGTTCGCTCCTTGGTCATCAAAAGGTATGGACAGAACTTTGCATCAGCTACTCCACGCAAGTTTTTTAAGAAGGTGAAGAATGCTCAGGAGGCCCATGAAGCAATCAGACCTACTGATGTTCAAAGATTTCCATCGTTGCTCAGAGGGATACTTGATGAAGATTCATTGAAGTTGTACACTCTTATTTGGTGTCGGACAGTTGCATGCCAAATGGAGCCTGCTACAATTGAGCAGATACAACTTGATATTGGAAATGCTGGTGAGTCCATTGTTTTCCGATCTGCATGCTCAAGAGTTCAGTTCCTTGGATATCAGGCAGTTTTTCAGGACGTGGAAGCTGGAGCCGTCAAATATAAAGAAAATGAAGGGATCAACCGGGATGAAGCTTTTGCACTTCTTGATTCACTGAAGGTTGGCAGTCCAGTGCATCTTGGTGACGTGGAACTCAAGCAACACCATACCCAACCACCTCCACGCTACTCTGAGGCATCATTGGTTAAGAAATTAGAGGAGCTTGGGATTGGAAGACCTTCAACATATGCAAGCACTATAAAGGTTTTACAGGACAGAAACTATCTGACAGTGAAAAGCCGTGTTTTACATCCAGAGTTTCGTGGTCGTATGGTTTCAGCATTTCTCTGCCACCATTTTTCTGAGGTCACAGATTATAGCTTCACAGCTGATATGGAGACCGAGCTTGATAATGTCTCTGCTGGCTTAACTGAATGGAAAGGCCTCCTAAGAGATTACTGGACTCGATTCAGTGTTTATTGTGAACGTACCAGTAATGTGACTATCCATCAGGTGGAGAAAATGATGGAAAAAAAATTTGGAGACTTCTTGTTTGCTACTCTCCCTGACCAAAGCAGGACATGTCCAAGCTGTAATGAAGGAACTTTGATCTTCAAAGTGAGTCGCTTCGGTGCGGGCTATTTCATAGGTTGTGATACACACCCAAAGTGCAAGTACATTGCTAAGACATTGTATGGTAATGAAGAAGAAGACGACAATACTCCGAAGAACAACAGGGTGGAGGAACCAAAATTGTTAGGTTTATCTCCCGGTTCCAATGAAAAGGTTCTTTTGAAGACTGGTCCTTATGGATCGTATGTACAACTTGGTGAAGACAAGAAGGGATACTCACCTAAAAGAGCCTCTATAGCACATATCAAGGATGTGAACTCAGTAACCGTTGAAGTTGCTCTCGAGTTGATGCGATATCCTGTGACATTGGGGAACCATCCGAAGGATGGGCTACCAGTGATTTTGAAGATTGCAAGGTCCGGATATATAGTTAAACATCGACGCACAATGGCTTCTCTAACGAAGAATCAGGAACCTAATAAATTAACCCTGGAGGACGCATTGGAACTTTTGTCTGGGAAAAATACACGGAAAATTGGTCGACCTAAGGGAAAATCAAAGAAACATTGGAATTTCGGAGCCAAACGCATCATCATCACCACCGTGTTTCGCTCCAATCCAATACCAACGATCATCATCAGGGGAAGGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

1155

Amino Acids

128.71

Weight (kDa)

9.52

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Toprim PF01751 325 - 438 5.2e-16 Toprim domain
Topoisom_bac PF01131 454 - 895 2.2e-112 DNA topoisomerase
Zn_ribbon_Top1 PF01396 939 - 975 6.6e-09 Topoisomerase DNA binding C4 zinc finger
Toprim_C_rpt PF13368 1003 - 1057 9.8e-09 Topoisomerase C-terminal repeat
Toprim_C_rpt PF13368 1063 - 1114 1.1e-07 Topoisomerase C-terminal repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 538
AccB7I CCANNNNNTGG 1 cut(s) 2752
AccI GTMKAC 2 cut(s) 801, 3352
AccII CGCG 1 cut(s) 235
AccIII TCCGGA 1 cut(s) 3230
AciI CCGC 6 cut(s) 233, 960, 1096, 1747, 1797, 2873
AclWI GGATC 5 cut(s) 589, 1096, 1202, 2324, 3058
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 868, 1369, 2774, 3382
AcuI CTGAAG 4 cut(s) 510, 576, 1769, 2375
AdeI CACNNNGTG 1 cut(s) 1397
AfaI GTAC 6 cut(s) 730, 956, 2123, 2728, 2915, 3060
AfiI CCNNNNNNNGG 8 cut(s) 102, 103, 965, 966, 1055, 2752, 3193, 3197
AflII CTTAAG 2 cut(s) 226, 1154
AflIII ACRYGT 1 cut(s) 2819
AjiI CACGTC 3 cut(s) 138, 2278, 2386
AjnI CCWGG 4 cut(s) 409, 928, 1142, 3300
AjuI GAANNNNNNNTTGG 8 cut(s) 452, 484, 534, 566, 580, 612, 2760, 2792
AleI CACNNNNGTG 1 cut(s) 3413
Alw21I GWGCWC 2 cut(s) 1342, 1402
Alw26I GTCTC 6 cut(s) 354, 628, 785, 2630, 2659, 2775
AlwI GGATC 5 cut(s) 589, 1096, 1202, 2324, 3058
AlwNI CAGNNNCTG 3 cut(s) 704, 1091, 1211
Ama87I CYCGRG 1 cut(s) 3152
Aor13HI TCCGGA 1 cut(s) 3230
AoxI GGCC 7 cut(s) 3, 158, 407, 1097, 1323, 2037, 2680
ApoI RAATTY 4 cut(s) 868, 1369, 2774, 3382
ArsI GACNNNNNNTTYG 4 cut(s) 632, 664, 2774, 2806
AseI ATTAAT 1 cut(s) 1701
Asp700I GAANNNNTTC 4 cut(s) 91, 1947, 2476, 3024
AspLEI GCGC 1 cut(s) 1618
AspS9I GGNCC 7 cut(s) 158, 1098, 1375, 1648, 2038, 3041, 3228
AsuC2I CCSGG 4 cut(s) 39, 1471, 2324, 3009
AvaI CYCGRG 1 cut(s) 3152
AvaII GGWCC 4 cut(s) 1375, 1648, 3041, 3228
AxyI CCTNAGG 1 cut(s) 3357
BaeGI GKGCMC 1 cut(s) 241
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 392
BarI GAAGNNNNNNTAC 2 cut(s) 938, 970
BbsI GAAGAC 5 cut(s) 48, 2479, 2955, 3041, 3078
Bbv12I GWGCWC 2 cut(s) 1342, 1402
BceAI ACGGC 4 cut(s) 37, 1409, 2276, 2523
BcgI CGANNNNNNTGC 4 cut(s) 1150, 1184, 2214, 2248
BciT130I CCWGG 4 cut(s) 411, 930, 1144, 3302
BciVI GTATCC 2 cut(s) 2088, 3077
BclI TGATCA 3 cut(s) 132, 1363, 1893
BcnI CCSGG 4 cut(s) 39, 1471, 2324, 3009
BcoDI GTCTC 6 cut(s) 354, 628, 785, 2630, 2659, 2775
BfaI CTAG 4 cut(s) 318, 1050, 1080, 1493
BfmI CTRYAG 5 cut(s) 11, 295, 693, 1343, 3105
BfrI CTTAAG 2 cut(s) 226, 1154
BfuAI ACCTGC 1 cut(s) 538
BfuI GTATCC 2 cut(s) 2088, 3077
BmcAI AGTACT 1 cut(s) 730
Bme1390I CCNGG 8 cut(s) 39, 411, 930, 1144, 1471, 2324, 3009, 3302
Bme18I GGWCC 4 cut(s) 1375, 1648, 3041, 3228
BmeT110I CYCGRG 1 cut(s) 3152
BmgBI CACGTC 3 cut(s) 138, 2278, 2386
BmgT120I GGNCC 7 cut(s) 158, 1098, 1375, 1648, 2038, 3041, 3228
BmiI GGNNCC 9 cut(s) 391, 1350, 2163, 2290, 2985, 3013, 3185, 3285, 3391
BmrFI CCNGG 8 cut(s) 39, 411, 930, 1144, 1471, 2324, 3009, 3302
BmrI ACTGGG 1 cut(s) 975
BmuI ACTGGG 1 cut(s) 975
BpiI GAAGAC 5 cut(s) 48, 2479, 2955, 3041, 3078
BpmI CTGGAG 4 cut(s) 45, 780, 2307, 3323
Bpu10I CCTNAGC 2 cut(s) 101, 2031
BpuEI CTTGAG 3 cut(s) 336, 2221, 2378
BpuMI CCSGG 4 cut(s) 39, 1471, 2324, 3009
BsaAI YACGTR 1 cut(s) 1277
BsaBI GATNNNNATC 3 cut(s) 1212, 2077, 3446
BsaI GGTCTC 2 cut(s) 628, 2630
BsaJI CCNNGG 3 cut(s) 1956, 2251, 3300
BsaWI WCCGGW 1 cut(s) 3230
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bsc4I CCNNNNNNNGG 8 cut(s) 102, 103, 965, 966, 1055, 2752, 3193, 3197
Bse1I ACTGG 9 cut(s) 62, 797, 970, 1462, 2369, 2702, 2730, 3043, 3206
Bse21I CCTNAGG 1 cut(s) 3357
Bse3DI GCAATG 1 cut(s) 2916
Bse8I GATNNNNATC 3 cut(s) 1212, 2077, 3446
BseAI TCCGGA 1 cut(s) 3230
BseBI CCWGG 4 cut(s) 411, 930, 1144, 3302
BseDI CCNNGG 3 cut(s) 1956, 2251, 3300
BseGI GGATG 9 cut(s) 574, 1273, 1798, 1936, 2332, 2548, 3127, 3187, 3202
BseJI GATNNNNATC 3 cut(s) 1212, 2077, 3446
BseLI CCNNNNNNNGG 8 cut(s) 102, 103, 965, 966, 1055, 2752, 3193, 3197
BseMI GCAATG 1 cut(s) 2916
BseMII CTCAG 6 cut(s) 1311, 2045, 2101, 2421, 2592, 3144
BseNI ACTGG 9 cut(s) 62, 797, 970, 1462, 2369, 2702, 2730, 3043, 3206
BseRI GAGGAG 1 cut(s) 2471
BseSI GKGCMC 1 cut(s) 241
BseYI CCCAGC 1 cut(s) 96
BsgI GTGCAG 3 cut(s) 762, 1203, 1473
Bsh1236I CGCG 1 cut(s) 235
BshFI GGCC 7 cut(s) 5, 160, 409, 1099, 1325, 2039, 2682
BsiHKAI GWGCWC 2 cut(s) 1342, 1402
BsiHKCI CYCGRG 1 cut(s) 3152
BsiSI CCGG 5 cut(s) 39, 1470, 2323, 3009, 3231
BslFI GGGAC 2 cut(s) 268, 1082
BslI CCNNNNNNNGG 8 cut(s) 102, 103, 965, 966, 1055, 2752, 3193, 3197
BsmAI GTCTC 6 cut(s) 354, 628, 785, 2630, 2659, 2775
BsmFI GGGAC 2 cut(s) 268, 1082
BsmI GAATGC 2 cut(s) 1603, 2032
BsnI GGCC 7 cut(s) 5, 160, 409, 1099, 1325, 2039, 2682
Bso31I GGTCTC 2 cut(s) 628, 2630
BsoBI CYCGRG 1 cut(s) 3152
Bsp1286I GDGCHC 4 cut(s) 241, 392, 1342, 1402
Bsp13I TCCGGA 1 cut(s) 3230
Bsp1407I TGTACA 2 cut(s) 2121, 3058
BspACI CCGC 6 cut(s) 233, 960, 1096, 1747, 1797, 2873
BspANI GGCC 7 cut(s) 5, 160, 409, 1099, 1325, 2039, 2682
BspCNI CTCAG 6 cut(s) 1312, 2044, 2100, 2422, 2593, 3143
BspEI TCCGGA 1 cut(s) 3230
BspFNI CGCG 1 cut(s) 235
BspHI TCATGA 1 cut(s) 1309
BspLI GGNNCC 9 cut(s) 391, 1350, 2163, 2290, 2985, 3013, 3185, 3285, 3391
BspMAI CTGCAG 2 cut(s) 15, 1347
BspMI ACCTGC 1 cut(s) 538
BspPI GGATC 5 cut(s) 589, 1096, 1202, 2324, 3058
BspTI CTTAAG 2 cut(s) 226, 1154
BspTNI GGTCTC 2 cut(s) 628, 2630
BsrDI GCAATG 1 cut(s) 2916
BsrGI TGTACA 2 cut(s) 2121, 3058
BsrI ACTGG 9 cut(s) 62, 797, 970, 1462, 2369, 2702, 2730, 3043, 3206
BssECI CCNNGG 3 cut(s) 1956, 2251, 3300
BssT1I CCWWGG 2 cut(s) 1956, 2251
Bst2UI CCWGG 4 cut(s) 411, 930, 1144, 3302
Bst4CI ACNGT 7 cut(s) 499, 976, 1434, 2146, 2530, 3139, 3415
BstAFI CTTAAG 2 cut(s) 226, 1154
BstAPI GCANNNNNTGC 3 cut(s) 704, 776, 1397
BstAUI TGTACA 2 cut(s) 2121, 3058
BstBAI YACGTR 1 cut(s) 1277
BstF5I GGATG 9 cut(s) 574, 1273, 1798, 1936, 2332, 2548, 3127, 3187, 3202
BstFNI CGCG 1 cut(s) 235
BstHHI GCGC 1 cut(s) 1618
BstMAI GTCTC 6 cut(s) 354, 628, 785, 2630, 2659, 2775
BstNI CCWGG 4 cut(s) 411, 930, 1144, 3302
BstNSI RCATGY 3 cut(s) 2154, 2235, 2823
BstSCI CCNGG 8 cut(s) 37, 409, 928, 1142, 1469, 2322, 3007, 3300
BstSFI CTRYAG 5 cut(s) 11, 295, 693, 1343, 3105
BstSLI GKGCMC 1 cut(s) 241
BstSNI TACGTA 1 cut(s) 1277
BstUI CGCG 1 cut(s) 235
BstV2I GAAGAC 5 cut(s) 48, 2479, 2955, 3041, 3078
BstX2I RGATCY 2 cut(s) 1088, 1207
BstXI CCANNNNNNTGG 1 cut(s) 1645
BstYI RGATCY 2 cut(s) 1088, 1207
Bsu36I CCTNAGG 1 cut(s) 3357
BsuI GTATCC 2 cut(s) 2088, 3077
BsuRI GGCC 7 cut(s) 5, 160, 409, 1099, 1325, 2039, 2682
BtrI CACGTC 3 cut(s) 138, 2278, 2386
BtsCI GGATG 9 cut(s) 574, 1273, 1798, 1936, 2332, 2548, 3127, 3187, 3202
BtsIMutI CAGTG 8 cut(s) 1317, 1599, 1822, 2351, 2376, 2535, 2716, 3213
BveI ACCTGC 1 cut(s) 538
CaiI CAGNNNCTG 3 cut(s) 704, 1091, 1211
CciI TCATGA 1 cut(s) 1309
CfoI GCGC 1 cut(s) 1618
Cfr13I GGNCC 7 cut(s) 158, 1098, 1375, 1648, 2038, 3041, 3228
CseI GACGC 2 cut(s) 3261, 3317
Csp6I GTAC 6 cut(s) 729, 955, 2122, 2727, 2914, 3059
CviAII CATG 8 cut(s) 130, 1061, 1310, 1379, 2042, 2151, 2232, 2820
CviQI GTAC 6 cut(s) 729, 955, 2122, 2727, 2914, 3059
DraI TTTAAA 2 cut(s) 1534, 1717
DraIII CACNNNGTG 1 cut(s) 1397
EaeI YGGCCR 1 cut(s) 3
Eco105I TACGTA 1 cut(s) 1277
Eco130I CCWWGG 2 cut(s) 1956, 2251
Eco147I AGGCCT 3 cut(s) 409, 1325, 2682
Eco24I GRGCYC 1 cut(s) 392
Eco31I GGTCTC 2 cut(s) 628, 2630
Eco32I GATATC 2 cut(s) 2258, 3167
Eco47I GGWCC 4 cut(s) 1375, 1648, 3041, 3228
Eco57I CTGAAG 4 cut(s) 510, 576, 1769, 2375
Eco81I CCTNAGG 1 cut(s) 3357
Eco88I CYCGRG 1 cut(s) 3152
EcoRII CCWGG 4 cut(s) 409, 928, 1142, 3300
EcoRV GATATC 2 cut(s) 2258, 3167
EcoT14I CCWWGG 2 cut(s) 1956, 2251
EcoT38I GRGCYC 1 cut(s) 392
ErhI CCWWGG 2 cut(s) 1956, 2251
FaeI CATG 8 cut(s) 133, 1064, 1313, 1382, 2045, 2154, 2235, 2823
FaqI GGGAC 2 cut(s) 268, 1082
FatI CATG 8 cut(s) 129, 1060, 1309, 1378, 2041, 2150, 2231, 2819
FauI CCCGC 2 cut(s) 967, 2866
FauNDI CATATG 1 cut(s) 2485
FbaI TGATCA 3 cut(s) 132, 1363, 1893
FblI GTMKAC 2 cut(s) 801, 3352
FokI GGATG 9 cut(s) 581, 1280, 1805, 1943, 2339, 2535, 3134, 3174, 3209
FriOI GRGCYC 1 cut(s) 392
FspBI CTAG 4 cut(s) 318, 1050, 1080, 1493
GlaI GCGC 1 cut(s) 1617
GsaI CCCAGC 1 cut(s) 100
GsuI CTGGAG 4 cut(s) 45, 780, 2307, 3323
HaeIII GGCC 7 cut(s) 5, 160, 409, 1099, 1325, 2039, 2682
HapII CCGG 5 cut(s) 39, 1470, 2323, 3009, 3231
HgaI GACGC 2 cut(s) 3261, 3317
HhaI GCGC 1 cut(s) 1618
Hin1II CATG 8 cut(s) 133, 1064, 1313, 1382, 2045, 2154, 2235, 2823
Hin6I GCGC 1 cut(s) 1616
HinP1I GCGC 1 cut(s) 1616
HincII GTYRAC 2 cut(s) 1884, 3353
HindII GTYRAC 2 cut(s) 1884, 3353
HindIII AAGCTT 5 cut(s) 575, 1710, 1835, 1847, 2331
HpaII CCGG 5 cut(s) 39, 1470, 2323, 3009, 3231
Hpy166II GTNNAC 6 cut(s) 802, 1884, 2124, 2723, 3127, 3353
Hpy8I GTNNAC 6 cut(s) 802, 1884, 2124, 2723, 3127, 3353
Hpy99I CGWCG 1 cut(s) 3255
HpyCH4III ACNGT 7 cut(s) 499, 976, 1434, 2146, 2530, 3139, 3415
HpyCH4IV ACGT 6 cut(s) 137, 202, 1276, 2277, 2385, 2725
HpySE526I ACGT 6 cut(s) 137, 202, 1276, 2277, 2385, 2725
Hsp92II CATG 8 cut(s) 133, 1064, 1313, 1382, 2045, 2154, 2235, 2823
HspAI GCGC 1 cut(s) 1616
Kpn2I TCCGGA 1 cut(s) 3230
Ksp22I TGATCA 3 cut(s) 132, 1363, 1893
MaeI CTAG 4 cut(s) 318, 1050, 1080, 1493
MaeII ACGT 6 cut(s) 137, 202, 1276, 2277, 2385, 2725
MaeIII GTNAC 7 cut(s) 882, 994, 2381, 2605, 2737, 3133, 3172
MfeI CAATTG 1 cut(s) 2172
MflI RGATCY 2 cut(s) 1088, 1207
MhlI GDGCHC 4 cut(s) 241, 392, 1342, 1402
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 8 cut(s) 280, 787, 998, 1141, 1485, 2219, 2695, 2869
MmeI TCCRAC 4 cut(s) 304, 532, 1626, 2120
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 3230
MroXI GAANNNNTTC 4 cut(s) 91, 1947, 2476, 3024
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 3 cut(s) 1815, 2379, 3413
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 875, 952, 2627
MspCI CTTAAG 2 cut(s) 226, 1154
MspI CCGG 5 cut(s) 39, 1470, 2323, 3009, 3231
MspR9I CCNGG 8 cut(s) 39, 411, 930, 1144, 1471, 2324, 3009, 3302
MunI CAATTG 1 cut(s) 2172
Mva1269I GAATGC 2 cut(s) 1603, 2032
MvaI CCWGG 4 cut(s) 411, 930, 1144, 3302
MvnI CGCG 1 cut(s) 235
NciI CCSGG 4 cut(s) 39, 1471, 2324, 3009
NdeI CATATG 1 cut(s) 2485
NlaIII CATG 8 cut(s) 133, 1064, 1313, 1382, 2045, 2154, 2235, 2823
NlaIV GGNNCC 9 cut(s) 391, 1350, 2163, 2290, 2985, 3013, 3185, 3285, 3391
NmuCI GTSAC 5 cut(s) 882, 2381, 2605, 2737, 3172
NspI RCATGY 3 cut(s) 2154, 2235, 2823
OliI CACNNNNGTG 1 cut(s) 3413
PaeI GCATGC 2 cut(s) 2154, 2235
PaeR7I CTCGAG 1 cut(s) 3152
PagI TCATGA 1 cut(s) 1309
PceI AGGCCT 3 cut(s) 409, 1325, 2682
PciI ACATGT 1 cut(s) 2819
PctI GAATGC 2 cut(s) 1603, 2032
PdmI GAANNNNTTC 4 cut(s) 91, 1947, 2476, 3024
PflMI CCANNNNNTGG 1 cut(s) 2752
PfoI TCCNGGA 1 cut(s) 928
PleI GAGTC 8 cut(s) 279, 787, 997, 1141, 1484, 2218, 2695, 2868
PpsI GAGTC 8 cut(s) 279, 787, 997, 1141, 1484, 2218, 2695, 2868
Ppu21I YACGTR 1 cut(s) 1277
PscI ACATGT 1 cut(s) 2819
PshBI ATTAAT 1 cut(s) 1701
Psp6I CCWGG 4 cut(s) 409, 928, 1142, 3300
PspFI CCCAGC 1 cut(s) 96
PspGI CCWGG 4 cut(s) 409, 928, 1142, 3300
PspN4I GGNNCC 9 cut(s) 391, 1350, 2163, 2290, 2985, 3013, 3185, 3285, 3391
PspPI GGNCC 7 cut(s) 158, 1098, 1375, 1648, 2038, 3041, 3228
PsrI GAACNNNNNNTAC 2 cut(s) 1932, 1964
PstI CTGCAG 2 cut(s) 15, 1347
PstNI CAGNNNCTG 3 cut(s) 704, 1091, 1211
PsuI RGATCY 2 cut(s) 1088, 1207
PvuII CAGCTG 3 cut(s) 875, 952, 2627
RsaI GTAC 6 cut(s) 730, 956, 2123, 2728, 2915, 3060
RsaNI GTAC 6 cut(s) 729, 955, 2122, 2727, 2914, 3059
RseI CAYNNNNRTG 3 cut(s) 1815, 2379, 3413
SalI GTCGAC 1 cut(s) 3351
Sau96I GGNCC 7 cut(s) 158, 1098, 1375, 1648, 2038, 3041, 3228
ScaI AGTACT 1 cut(s) 730
SchI GAGTC 8 cut(s) 280, 787, 998, 1141, 1485, 2219, 2695, 2869
ScrFI CCNGG 8 cut(s) 39, 411, 930, 1144, 1471, 2324, 3009, 3302
SduI GDGCHC 4 cut(s) 241, 392, 1342, 1402
SfcI CTRYAG 5 cut(s) 11, 295, 693, 1343, 3105
Sfr274I CTCGAG 1 cut(s) 3152
SinI GGWCC 4 cut(s) 1375, 1648, 3041, 3228
SlaI CTCGAG 1 cut(s) 3152
SmiMI CAYNNNNRTG 3 cut(s) 1815, 2379, 3413
SmlI CTYRAG 6 cut(s) 226, 351, 1154, 2236, 2393, 3152
SmoI CTYRAG 6 cut(s) 226, 351, 1154, 2236, 2393, 3152
SnaBI TACGTA 1 cut(s) 1277
SphI GCATGC 2 cut(s) 2154, 2235
SseBI AGGCCT 3 cut(s) 409, 1325, 2682
SsiI CCGC 6 cut(s) 233, 960, 1096, 1747, 1797, 2873
SspI AATATT 2 cut(s) 1426, 1547
SspMI CTAG 4 cut(s) 318, 1050, 1080, 1493
StuI AGGCCT 3 cut(s) 409, 1325, 2682
StyD4I CCNGG 8 cut(s) 37, 409, 928, 1142, 1469, 2322, 3007, 3300
StyI CCWWGG 2 cut(s) 1956, 2251
TaaI ACNGT 7 cut(s) 499, 976, 1434, 2146, 2530, 3139, 3415
TaiI ACGT 6 cut(s) 140, 205, 1279, 2280, 2388, 2728
TaqI TCGA 9 cut(s) 302, 503, 650, 710, 1524, 2704, 3153, 3250, 3352
TaqII GACCGA 1 cut(s) 2654
TatI WGTACW 4 cut(s) 728, 2121, 2913, 3058
TauI GCSGC 2 cut(s) 1099, 1799
TscAI CASTG 8 cut(s) 1324, 1599, 1822, 2358, 2376, 2535, 2716, 3213
TseFI GTSAC 5 cut(s) 882, 2381, 2605, 2737, 3172
Tsp45I GTSAC 5 cut(s) 882, 2381, 2605, 2737, 3172
TspGWI ACGGA 1 cut(s) 3355
TspRI CASTG 8 cut(s) 1324, 1599, 1822, 2358, 2376, 2535, 2716, 3213
Van91I CCANNNNNTGG 1 cut(s) 2752
Vha464I CTTAAG 2 cut(s) 226, 1154
VpaK11BI GGWCC 4 cut(s) 1375, 1648, 3041, 3228
VspI ATTAAT 1 cut(s) 1701
XapI RAATTY 4 cut(s) 868, 1369, 2774, 3382
XbaI TCTAGA 1 cut(s) 317
XceI RCATGY 3 cut(s) 2154, 2235, 2823
XcmI CCANNNNNNNNNTGG 3 cut(s) 1140, 1545, 3194
XhoI CTCGAG 1 cut(s) 3152
XmiI GTMKAC 2 cut(s) 801, 3352
XmnI GAANNNNTTC 4 cut(s) 91, 1947, 2476, 3024
XspI CTAG 4 cut(s) 318, 1050, 1080, 1493
ZrmI AGTACT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.