MD15G1105700.v1.1

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006256
Physical Location & Seq
Reverse (-)
7382325 .. 7392565
10241 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1105700.v1.1.491

Sequence Viewer

Length: 3534 bp
ATGGCGATGATACATTTTCAACGGCTGTCCACCGCCCCCGCTCCCGCCACCGCTTCCTCCTTCATTTTCTCAAACACTTTTGCTGCCTCCGCCCTGTGTCTCCGCCGCTCCATGGCCAAGCTGCAGTGGAGGGTGTTTGAGGCTTATTCATGCGCTTGCTTACCATCTTCGTCAAATTTGACAACGAATGTATACAGAAATTATAGGTTAAATAAGTCCGGAGTTTCTTGCATGATCACGGCCAACCATTCAAAGTTTGGGCCAAATTTTCATCCTTTCAGAAGAAGTGGCGATGGGTCTGAATGCGCTTTTACATTTCATTCTCATCTTAAACATGGTTCGCTTAATTTTTTAGGGCCTGCTGGTATTGTCAATTGTGGCTCTTTTAAGAATAGTTGCAGATTGGGTTTAACTAATCATAGTGCTGGAGCTTTTCCCAAGAGGTTTTTCTCTCAAGTTCCATCTGTGATAAACAAAGGAGAGAGTGTGGGTGTCCAAGATGGTAGGAATAGTGGAAATACGTTTTTTTTCAGAAGGCTTATTAAATGCAAGAAACGGATAAAGGCATTGGCAGCTCAAAGCAAAACCAAAGCTACTGATTCTAGTATCTCTAAAGATGTTGAAGTAGAAGCCTTAACCGAACAGCAAGTTGGAAGTGAGAAGGGGGCTGGAAAATTGGGATCTTTGTGTTCTAGTTCTCCTATAAGAAATGATACCCGGGTCTCAAAAGTCAAAGGGAAAAAGAGGTCGAAGAGCAAGAAAACTAAAGAACAAATTTCTGCTGCAAATCCTCCAGCAGAAGCTGCTATGTCACAAAGTTCTAAGGTTACTTCTCAACCAAAGAAATCTGGTGGTACCAAAAGTGGCAAACAGTCGGCGGAAGTTTCAGAGTTGAACCTCACTGCGAAAACTCCCGTGGAAATTGTTGATGTTTCTTCAACAAAATCCCAGCCAAAGAAGAAAGGTGGGAACTCTTTTAGAAAGGGAAAATTAATAAATTCAGATACTAAGTCGCCACAGAAACTTATACCACAAAAAATAGGCAAAATGAAACCTCATGGGAAAATGACACTGAAGCAGTTGTACCCCGCAACTGGGAAAACTGTAGTGGTGGTGGAATCTGCTACAAAGGCGAAGGTTATTCAGGGTTATCTTGATGATATGTTTGAAGTTCTTCCTAGCTATGGTCATGTAAGGGACTTGGCTGCTAGGTCTGGATCTGTACGGCCTGATGATGATTTTAGTATGGTGTGGGAAGTTCCATCTGCCGCCTGGACTCATCTAAATAGCATAAAGGTTGCACTAACAGGAGCAGAAACTCTTATTCTTGCATCAGATCCTGATCGTGAAGGAGAGGCTATTGCTTGGCATATTATTGAGATGCTGCAACAACAGGATGCTTTACGTGAAGATGTTACTGTAGCCAGGGTTGTTTTTCATGAAATCACTGAGGCCTCTATCAAAAGCGCGCTGCAAGCTCCAAGAGAAATTGATGAGAACTTGGTCCATGCTTACCTTGCACGGCGTGCACTTGATTATTTGATTGGATTTAAAATTTCACCACTGTTGTGGAGGAAACTACCAGGTTGCCAGTCTGCGGGGCGAGTCCAATCTGCTGCACTGGCTCTTCTGTGCGATAGAGAAATGGAAATTGATGAATTTAAACCACAGGAATATTGGACTACTGCGGTTGAATTGAAGAAAAAAGAAAAGGATTCATCAGTTAATGCTCCATCCTTCCCAGCATACTTGACCCATTATGATTCCAAAAAGCTAAATCAATTTTCTATCAGTTCTCATACCGAAGCAAAAGAAATTGAACAGCAGATTAATTCAGAAAATTTTCAAGTTGTTAATTCTAAGAAAAACAAAATGCGGAAGAATCCTCCCACGCCGTATATAACATCCACACTTCAGCAGGATGCTGCCAACAAATTACATTTCAGTGCAGCATATACAATGAAGCTTGCACAGAAACTTTACGAGGGGGTTCAGTTATCTGATGGTAAAGCAACGGGTTTGATCACATACACCCGAACAGATGGACTACATATTTCTGATGAAGCCTGTAAGGATGTGCGCTCCTTGGTCATTGAAAGGTATGGACAGAATTTTGCATCAGAAAGTGCACGCAAATTTTTTAAGAAAGTGAAGAATTCCCAAGAGGCCCATGAAGCAATCAGACCCACTGATGTTCGAAGATTGCCATCATTGCTCCGCGGGGTACTTGATGAAGATTCCATGAAGTTGTACACTCTTATTTGGTGTCGGACAGTGGCATGTCAGATGGAGCCTGTTACAATTGAGCAGATACAACTCAATATTGGAAATGCTCACAACTCCATTGTTCTCCGCTCTTTGTCCTCAAGGGTTGAGTTCCCTGGATATCAGGCAGTCTTGGAGGACATGGAAGCTCAAGCAGTCAAATATAAAGAACATGAAGGGAACAGTCATGACGAAGCTTTTGTACTTCTTAATTCATTGAAGTATGGGGATCCATTAGATATTGGTAAAGTGGAACTCAAGGAGCACCGTACTCAACCTCCTCCCCGCTACTCTGAGGCAGCACTGATTAAGAAATTGGAGGAGCTTGGGATTGGAAGACCTTCAACATATGCGAGCACTCTGAAGGTTTTACAGGGTAGAAACTATGTGACAGTGAAAAGCCGTGTATTACATCCAGAATTTCGTGGTCGTATGGTGTCAGCATTTCTCGGCAACCATTTTTCCGAGGTCACAGATTATAGTTTTACTGCTGACATGGAGACTGAGCTTGATAATGTCTCTGCTGGCTTGACTGAATGGAAAGGCCTCCTTAAAGATTATTGGACCCGATTCAGCACTTATTGTGAACGTACCAGTACTGTTCATATTCATCAGGTAGAGAAGATGATGGAGAAGAAATTTGGAGACTTCTTGTTTGCTTCTCTTCCCGACCAAAGCAGGACATGCCCATATTGTGCGGAAGGCAGTTTGATTTTCAAAGTGAGTCGGTTCGGTGCTGGCTATTTTATAGGTTGTGATCAACACCCACAGTGCAAATACATTGCTAAGACATTTTACAGTGATGAAGAAGAAGAAGAAGAAGAAGACGGTCAAAAAAAGAACAGCGGCATGGAGGAACCAAAACTGCTAGGTCTAAGTCCAGTTTCCAGTGAAAAGGTTCTTTTGAAGAAAGGTCCTTACGGATGCTATGTACAACTTGGTGAAGACAGGAAGACTTACGTACCTAAAAGAGCCAATGTATCCCATATAAAGGATGTGAACTCGATTACCCTTGAATATGCGCTGGAGTTGCTGCGATATCCTGCGACATTGGGAAAACATCCAGCTGATGGACAACCAGTCATTTTAAAGGTTTCAAAGGTTGGGTTTGCAGTTAGACATCGGCGCACAATTGCTTCTGTTCCTAAGTATAGCAAACCCACTGAAATCACCCTCGAGGGTGCATTGAAGCTTTTGTCTGGTAAGGATGTAAGAAAAATTGGTCGACCTACGAGAGAACAAATTGAAGAAGCCCAAGCAGCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

1178

Amino Acids

130.92

Weight (kDa)

9.32

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Toprim PF01751 368 - 482 2.1e-16 Toprim domain
Topoisom_bac PF01131 497 - 939 1.8e-110 DNA topoisomerase
Zn_ribbon_Top1 PF01396 982 - 1017 1.8e-07 Topoisomerase DNA binding C4 zinc finger
Toprim_C_rpt PF13368 1049 - 1110 1.2e-10 Topoisomerase C-terminal repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 3440
Acc65I GGTACC 1 cut(s) 854
AccB1I GGYRCC 1 cut(s) 854
AccB7I CCANNNNNTGG 1 cut(s) 3335
AccBSI CCGCTC 3 cut(s) 41, 108, 2355
AccI GTMKAC 2 cut(s) 192, 3490
AccII CGCG 2 cut(s) 1469, 2220
AccIII TCCGGA 1 cut(s) 218
AclWI GGATC 5 cut(s) 688, 1225, 1331, 2489, 2502
AcoI YGGCCR 2 cut(s) 114, 240
AcuI CTGAAG 3 cut(s) 1094, 1898, 2648
AdeI CACNNNGTG 1 cut(s) 1526
AfiI CCNNNNNNNGG 7 cut(s) 112, 1094, 1095, 1184, 1597, 3256, 3335
AhdI GACNNNNNGTC 1 cut(s) 3344
AjnI CCWGG 4 cut(s) 1271, 1424, 1582, 2380
AjuI GAANNNNNNNTTGG 4 cut(s) 431, 463, 633, 665
AleI CACNNNNGTG 1 cut(s) 1567
Alw21I GWGCWC 4 cut(s) 1531, 2131, 2532, 2624
Alw26I GTCTC 5 cut(s) 104, 727, 2759, 2788, 2904
Alw44I GTGCAC 2 cut(s) 1527, 2127
AlwI GGATC 5 cut(s) 688, 1225, 1331, 2489, 2502
AlwNI CAGNNNCTG 2 cut(s) 803, 1340
Ama87I CYCGRG 2 cut(s) 717, 3440
Aor13HI TCCGGA 1 cut(s) 218
AoxI GGCC 8 cut(s) 114, 240, 260, 356, 1226, 1452, 2166, 2809
ApaLI GTGCAC 2 cut(s) 1527, 2127
ArsI GACNNNNNNTTYG 4 cut(s) 2447, 2479, 2903, 2935
AseI ATTAAT 2 cut(s) 992, 1830
Asp700I GAANNNNTTC 4 cut(s) 1173, 1842, 2605, 3162
Asp718I GGTACC 1 cut(s) 854
AspLEI GCGC 7 cut(s) 155, 308, 1469, 1471, 2082, 3289, 3393
AspS9I GGNCC 6 cut(s) 260, 356, 1504, 2167, 2829, 3179
AsuC2I CCSGG 2 cut(s) 718, 719
AsuHPI GGTGA 3 cut(s) 1551, 3218, 3427
AsuII TTCGAA 1 cut(s) 2197
AvaI CYCGRG 2 cut(s) 717, 3440
AvaII GGWCC 3 cut(s) 1504, 2829, 3179
BaeGI GKGCMC 2 cut(s) 1531, 2131
BaeI ACNNNNGTAYC 4 cut(s) 705, 738, 3210, 3243
BalI TGGCCA 1 cut(s) 116
BamHI GGATCC 1 cut(s) 2494
BanI GGYRCC 1 cut(s) 854
BarI GAAGNNNNNNTAC 4 cut(s) 1067, 1099, 2451, 2483
BbsI GAAGAC 4 cut(s) 2608, 3096, 3216, 3224
Bbv12I GWGCWC 4 cut(s) 1531, 2131, 2532, 2624
BceAI ACGGC 6 cut(s) 38, 255, 1241, 1538, 1879, 2652
BciT130I CCWGG 4 cut(s) 1273, 1426, 1584, 2382
BciVI GTATCC 1 cut(s) 3256
BclI TGATCA 3 cut(s) 234, 2022, 3022
BcnI CCSGG 2 cut(s) 718, 719
BcoDI GTCTC 5 cut(s) 104, 727, 2759, 2788, 2904
BfaI CTAG 5 cut(s) 603, 693, 1179, 1209, 3134
BfmI CTRYAG 3 cut(s) 122, 1104, 1419
BfuI GTATCC 1 cut(s) 3256
BmcAI AGTACT 1 cut(s) 2863
Bme1390I CCNGG 6 cut(s) 718, 719, 1273, 1426, 1584, 2382
Bme18I GGWCC 3 cut(s) 1504, 2829, 3179
BmeRI GACNNNNNGTC 1 cut(s) 3344
BmeT110I CYCGRG 2 cut(s) 717, 3440
BmgT120I GGNCC 6 cut(s) 260, 356, 1504, 2167, 2829, 3179
BmiI GGNNCC 5 cut(s) 856, 2292, 2496, 2831, 3123
BmrFI CCNGG 6 cut(s) 718, 719, 1273, 1426, 1584, 2382
BmrI ACTGGG 1 cut(s) 1104
BmsI GCATC 6 cut(s) 1340, 1371, 1387, 1912, 2126, 3179
BmuI ACTGGG 1 cut(s) 1104
BpiI GAAGAC 4 cut(s) 2608, 3096, 3216, 3224
BpmI CTGGAG 3 cut(s) 447, 777, 3311
Bpu14I TTCGAA 1 cut(s) 2197
BpuEI CTTGAG 4 cut(s) 438, 2350, 2400, 2507
BpuMI CCSGG 2 cut(s) 718, 719
BsaAI YACGTR 2 cut(s) 1406, 3226
BsaBI GATNNNNATC 2 cut(s) 1341, 2206
BsaI GGTCTC 1 cut(s) 727
BsaJI CCNNGG 8 cut(s) 111, 717, 915, 1425, 2085, 2218, 2380, 2730
BsaWI WCCGGW 1 cut(s) 218
BsaXI ACNNNNNCTCC 4 cut(s) 471, 501, 2527, 2557
Bsc4I CCNNNNNNNGG 7 cut(s) 112, 1094, 1095, 1184, 1597, 3256, 3335
Bse1I ACTGG 7 cut(s) 1099, 1591, 1626, 2859, 3146, 3153, 3344
Bse3DI GCAATG 2 cut(s) 2210, 3045
Bse8I GATNNNNATC 2 cut(s) 1341, 2206
BseAI TCCGGA 1 cut(s) 218
BseBI CCWGG 4 cut(s) 1273, 1426, 1584, 2382
BseDI CCNNGG 8 cut(s) 111, 717, 915, 1425, 2085, 2218, 2380, 2730
BseJI GATNNNNATC 2 cut(s) 1341, 2206
BseLI CCNNNNNNNGG 7 cut(s) 112, 1094, 1095, 1184, 1597, 3256, 3335
BseMI GCAATG 2 cut(s) 2210, 3045
BseMII CTCAG 3 cut(s) 1440, 2550, 2760
BseNI ACTGG 7 cut(s) 1099, 1591, 1626, 2859, 3146, 3153, 3344
BsePI GCGCGC 1 cut(s) 1467
BseRI GAGGAG 2 cut(s) 2535, 2600
BseSI GKGCMC 2 cut(s) 1531, 2131
BseYI CCCAGC 2 cut(s) 948, 1741
BsgI GTGCAG 2 cut(s) 1602, 1968
Bsh1236I CGCG 2 cut(s) 1469, 2220
BshFI GGCC 8 cut(s) 116, 242, 262, 358, 1228, 1454, 2168, 2811
BshNI GGYRCC 1 cut(s) 854
BsiHKAI GWGCWC 4 cut(s) 1531, 2131, 2532, 2624
BsiHKCI CYCGRG 2 cut(s) 717, 3440
BsiSI CCGG 2 cut(s) 219, 718
BslFI GGGAC 1 cut(s) 1211
BslI CCNNNNNNNGG 7 cut(s) 112, 1094, 1095, 1184, 1597, 3256, 3335
BsmAI GTCTC 5 cut(s) 104, 727, 2759, 2788, 2904
BsmFI GGGAC 1 cut(s) 1211
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 8 cut(s) 116, 242, 262, 358, 1228, 1454, 2168, 2811
Bso31I GGTCTC 1 cut(s) 727
BsoBI CYCGRG 2 cut(s) 717, 3440
Bsp119I TTCGAA 1 cut(s) 2197
Bsp1286I GDGCHC 4 cut(s) 1531, 2131, 2532, 2624
Bsp13I TCCGGA 1 cut(s) 218
Bsp1407I TGTACA 2 cut(s) 2250, 3196
Bsp143I GATC 8 cut(s) 234, 680, 1217, 1336, 1342, 2022, 2494, 3022
Bsp19I CCATGG 1 cut(s) 111
BspANI GGCC 8 cut(s) 116, 242, 262, 358, 1228, 1454, 2168, 2811
BspCNI CTCAG 3 cut(s) 1441, 2551, 2761
BspEI TCCGGA 1 cut(s) 218
BspFNI CGCG 2 cut(s) 1469, 2220
BspHI TCATGA 2 cut(s) 1438, 2452
BspLI GGNNCC 5 cut(s) 856, 2292, 2496, 2831, 3123
BspMAI CTGCAG 1 cut(s) 126
BspPI GGATC 5 cut(s) 688, 1225, 1331, 2489, 2502
BspQI GCTCTTC 2 cut(s) 746, 1632
BspT104I TTCGAA 1 cut(s) 2197
BspT107I GGYRCC 1 cut(s) 854
BspTNI GGTCTC 1 cut(s) 727
BsrBI CCGCTC 3 cut(s) 41, 108, 2355
BsrDI GCAATG 2 cut(s) 2210, 3045
BsrGI TGTACA 2 cut(s) 2250, 3196
BsrI ACTGG 7 cut(s) 1099, 1591, 1626, 2859, 3146, 3153, 3344
BssECI CCNNGG 8 cut(s) 111, 717, 915, 1425, 2085, 2218, 2380, 2730
BssHII GCGCGC 1 cut(s) 1467
BssMI GATC 8 cut(s) 234, 680, 1217, 1336, 1342, 2022, 2494, 3022
BssNAI GTATAC 1 cut(s) 193
BssT1I CCWWGG 2 cut(s) 111, 2085
Bst1107I GTATAC 1 cut(s) 193
Bst2UI CCWGG 4 cut(s) 1273, 1426, 1584, 2382
Bst6I CTCTTC 3 cut(s) 746, 1632, 2934
BstAPI GCANNNNNTGC 3 cut(s) 803, 1526, 2949
BstAUI TGTACA 2 cut(s) 2250, 3196
BstBAI YACGTR 2 cut(s) 1406, 3226
BstBI TTCGAA 1 cut(s) 2197
BstDEI CTNAG 9 cut(s) 822, 1008, 1449, 1860, 2559, 2769, 3051, 3140, 3411
BstDSI CCRYGG 3 cut(s) 111, 915, 2218
BstFNI CGCG 2 cut(s) 1469, 2220
BstHHI GCGC 7 cut(s) 155, 308, 1469, 1471, 2082, 3289, 3393
BstKTI GATC 8 cut(s) 237, 683, 1220, 1339, 1345, 2025, 2497, 3025
BstMAI GTCTC 5 cut(s) 104, 727, 2759, 2788, 2904
BstMBI GATC 8 cut(s) 234, 680, 1217, 1336, 1342, 2022, 2494, 3022
BstNI CCWGG 4 cut(s) 1273, 1426, 1584, 2382
BstNSI RCATGY 2 cut(s) 2283, 2952
BstSCI CCNGG 6 cut(s) 716, 717, 1271, 1424, 1582, 2380
BstSFI CTRYAG 3 cut(s) 122, 1104, 1419
BstSLI GKGCMC 2 cut(s) 1531, 2131
BstSNI TACGTA 1 cut(s) 3226
BstUI CGCG 2 cut(s) 1469, 2220
BstV2I GAAGAC 4 cut(s) 2608, 3096, 3216, 3224
BstX2I RGATCY 4 cut(s) 680, 1217, 1336, 2494
BstXI CCANNNNNNTGG 1 cut(s) 1569
BstYI RGATCY 4 cut(s) 680, 1217, 1336, 2494
BstZ17I GTATAC 1 cut(s) 193
BsuI GTATCC 1 cut(s) 3256
BsuRI GGCC 8 cut(s) 116, 242, 262, 358, 1228, 1454, 2168, 2811
BtgI CCRYGG 3 cut(s) 111, 915, 2218
BtgZI GCGATG 2 cut(s) 20, 306
BtsI GCAGTG 2 cut(s) 131, 900
CaiI CAGNNNCTG 2 cut(s) 803, 1340
CciI TCATGA 2 cut(s) 1438, 2452
CfoI GCGC 7 cut(s) 155, 308, 1469, 1471, 2082, 3289, 3393
Cfr13I GGNCC 6 cut(s) 260, 356, 1504, 2167, 2829, 3179
Cfr42I CCGCGG 1 cut(s) 2221
Cfr9I CCCGGG 1 cut(s) 717
CsiI ACCWGGT 1 cut(s) 1582
DdeI CTNAG 9 cut(s) 822, 1008, 1449, 1860, 2559, 2769, 3051, 3140, 3411
DpnI GATC 8 cut(s) 236, 682, 1219, 1338, 1344, 2024, 2496, 3024
DpnII GATC 8 cut(s) 234, 680, 1217, 1336, 1342, 2022, 2494, 3022
DraI TTTAAA 3 cut(s) 1552, 1663, 3354
DraIII CACNNNGTG 1 cut(s) 1526
DriI GACNNNNNGTC 1 cut(s) 3344
EaeI YGGCCR 2 cut(s) 114, 240
Eam1104I CTCTTC 3 cut(s) 746, 1632, 2934
Eam1105I GACNNNNNGTC 1 cut(s) 3344
EarI CTCTTC 3 cut(s) 746, 1632, 2934
EciI GGCGGA 3 cut(s) 79, 92, 893
Eco105I TACGTA 1 cut(s) 3226
Eco130I CCWWGG 2 cut(s) 111, 2085
Eco147I AGGCCT 2 cut(s) 1454, 2811
Eco31I GGTCTC 1 cut(s) 727
Eco32I GATATC 2 cut(s) 2387, 3305
Eco47I GGWCC 3 cut(s) 1504, 2829, 3179
Eco57I CTGAAG 3 cut(s) 1094, 1898, 2648
Eco88I CYCGRG 2 cut(s) 717, 3440
EcoO109I RGGNCCY 2 cut(s) 356, 3179
EcoRI GAATTC 1 cut(s) 2155
EcoRII CCWGG 4 cut(s) 1271, 1424, 1582, 2380
EcoRV GATATC 2 cut(s) 2387, 3305
EcoT14I CCWWGG 2 cut(s) 111, 2085
ErhI CCWWGG 2 cut(s) 111, 2085
FalI AAGNNNNNCTT 2 cut(s) 2799, 2831
FaqI GGGAC 1 cut(s) 1211
FauI CCCGC 6 cut(s) 46, 52, 1096, 1591, 2213, 2558
FauNDI CATATG 1 cut(s) 2614
FbaI TGATCA 3 cut(s) 234, 2022, 3022
FblI GTMKAC 2 cut(s) 192, 3490
FspBI CTAG 5 cut(s) 603, 693, 1179, 1209, 3134
GlaI GCGC 7 cut(s) 154, 307, 1468, 1470, 2081, 3288, 3392
GsaI CCCAGC 2 cut(s) 952, 1745
GsuI CTGGAG 3 cut(s) 447, 777, 3311
HaeIII GGCC 8 cut(s) 116, 242, 262, 358, 1228, 1454, 2168, 2811
HapII CCGG 2 cut(s) 219, 718
HhaI GCGC 7 cut(s) 155, 308, 1469, 1471, 2082, 3289, 3393
Hin6I GCGC 7 cut(s) 153, 306, 1467, 1469, 2080, 3287, 3391
HinP1I GCGC 7 cut(s) 153, 306, 1467, 1469, 2080, 3287, 3391
HincII GTYRAC 1 cut(s) 3491
HindII GTYRAC 1 cut(s) 3491
HindIII AAGCTT 3 cut(s) 1964, 2460, 3455
HpaII CCGG 2 cut(s) 219, 718
HphI GGTGA 3 cut(s) 1551, 3218, 3427
Hpy166II GTNNAC 8 cut(s) 30, 193, 1529, 2129, 2253, 2852, 3265, 3491
Hpy188III TCNNGA 9 cut(s) 219, 1154, 1215, 1340, 1346, 1439, 2453, 2681, 2933
Hpy8I GTNNAC 8 cut(s) 30, 193, 1529, 2129, 2253, 2852, 3265, 3491
HpyCH4IV ACGT 4 cut(s) 521, 1405, 2854, 3225
HpyF3I CTNAG 9 cut(s) 822, 1008, 1449, 1860, 2559, 2769, 3051, 3140, 3411
HpySE526I ACGT 4 cut(s) 521, 1405, 2854, 3225
HspAI GCGC 7 cut(s) 153, 306, 1467, 1469, 2080, 3287, 3391
Kpn2I TCCGGA 1 cut(s) 218
KpnI GGTACC 1 cut(s) 858
Ksp22I TGATCA 3 cut(s) 234, 2022, 3022
KspI CCGCGG 1 cut(s) 2221
Kzo9I GATC 8 cut(s) 234, 680, 1217, 1336, 1342, 2022, 2494, 3022
LguI GCTCTTC 2 cut(s) 746, 1632
LweI GCATC 6 cut(s) 1340, 1371, 1387, 1912, 2126, 3179
MabI ACCWGGT 1 cut(s) 1582
MaeI CTAG 5 cut(s) 603, 693, 1179, 1209, 3134
MaeII ACGT 4 cut(s) 521, 1405, 2854, 3225
MaeIII GTNAC 6 cut(s) 810, 826, 1414, 2296, 2653, 2734
MalI GATC 8 cut(s) 236, 682, 1219, 1338, 1344, 2024, 2496, 3024
MbiI CCGCTC 3 cut(s) 41, 108, 2355
MboI GATC 8 cut(s) 234, 680, 1217, 1336, 1342, 2022, 2494, 3022
MfeI CAATTG 3 cut(s) 373, 2301, 3396
MflI RGATCY 4 cut(s) 680, 1217, 1336, 2494
MhlI GDGCHC 4 cut(s) 1531, 2131, 2532, 2624
MlsI TGGCCA 1 cut(s) 116
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 3 cut(s) 1270, 1614, 2998
MmeI TCCRAC 2 cut(s) 631, 2249
Mox20I TGGCCA 1 cut(s) 116
MroI TCCGGA 1 cut(s) 218
MroXI GAANNNNTTC 4 cut(s) 1173, 1842, 2605, 3162
MscI TGGCCA 1 cut(s) 116
MslI CAYNNNNRTG 2 cut(s) 1567, 1944
Msp20I TGGCCA 1 cut(s) 116
MspA1I CMGCKG 3 cut(s) 2220, 3111, 3332
MspI CCGG 2 cut(s) 219, 718
MspR9I CCNGG 6 cut(s) 718, 719, 1273, 1426, 1584, 2382
MunI CAATTG 3 cut(s) 373, 2301, 3396
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 4 cut(s) 1273, 1426, 1584, 2382
MvnI CGCG 2 cut(s) 1469, 2220
NciI CCSGG 2 cut(s) 718, 719
NcoI CCATGG 1 cut(s) 111
NdeI CATATG 1 cut(s) 2614
NdeII GATC 8 cut(s) 234, 680, 1217, 1336, 1342, 2022, 2494, 3022
NlaIV GGNNCC 5 cut(s) 856, 2292, 2496, 2831, 3123
NmeAIII GCCGAG 1 cut(s) 2694
NmuCI GTSAC 3 cut(s) 810, 2653, 2734
NspI RCATGY 2 cut(s) 2283, 2952
NspV TTCGAA 1 cut(s) 2197
OliI CACNNNNGTG 1 cut(s) 1567
PaeR7I CTCGAG 1 cut(s) 3440
PagI TCATGA 2 cut(s) 1438, 2452
PauI GCGCGC 1 cut(s) 1467
PceI AGGCCT 2 cut(s) 1454, 2811
PciSI GCTCTTC 2 cut(s) 746, 1632
PctI GAATGC 1 cut(s) 308
PdmI GAANNNNTTC 4 cut(s) 1173, 1842, 2605, 3162
PfeI GAWTC 7 cut(s) 599, 1118, 1715, 1763, 1882, 2237, 2835
PflMI CCANNNNNTGG 1 cut(s) 3335
PleI GAGTC 3 cut(s) 1270, 1613, 2997
PpsI GAGTC 3 cut(s) 1270, 1613, 2997
Ppu21I YACGTR 2 cut(s) 1406, 3226
PpuMI RGGWCCY 1 cut(s) 3179
PshBI ATTAAT 2 cut(s) 992, 1830
Psp5II RGGWCCY 1 cut(s) 3179
Psp6I CCWGG 4 cut(s) 1271, 1424, 1582, 2380
PspFI CCCAGC 2 cut(s) 948, 1741
PspGI CCWGG 4 cut(s) 1271, 1424, 1582, 2380
PspN4I GGNNCC 5 cut(s) 856, 2292, 2496, 2831, 3123
PspPI GGNCC 6 cut(s) 260, 356, 1504, 2167, 2829, 3179
PspPPI RGGWCCY 1 cut(s) 3179
PspXI VCTCGAGB 1 cut(s) 3440
PstI CTGCAG 1 cut(s) 126
PstNI CAGNNNCTG 2 cut(s) 803, 1340
PsuI RGATCY 4 cut(s) 680, 1217, 1336, 2494
PteI GCGCGC 1 cut(s) 1467
PvuII CAGCTG 1 cut(s) 3332
RseI CAYNNNNRTG 2 cut(s) 1567, 1944
SacII CCGCGG 1 cut(s) 2221
SalI GTCGAC 1 cut(s) 3489
SapI GCTCTTC 2 cut(s) 746, 1632
Sau3AI GATC 8 cut(s) 234, 680, 1217, 1336, 1342, 2022, 2494, 3022
Sau96I GGNCC 6 cut(s) 260, 356, 1504, 2167, 2829, 3179
ScaI AGTACT 1 cut(s) 2863
SchI GAGTC 3 cut(s) 1270, 1614, 2998
ScrFI CCNGG 6 cut(s) 718, 719, 1273, 1426, 1584, 2382
SduI GDGCHC 4 cut(s) 1531, 2131, 2532, 2624
SexAI ACCWGGT 1 cut(s) 1582
SfaNI GCATC 6 cut(s) 1340, 1371, 1387, 1912, 2126, 3179
SfcI CTRYAG 3 cut(s) 122, 1104, 1419
Sfr274I CTCGAG 1 cut(s) 3440
Sfr303I CCGCGG 1 cut(s) 2221
SfuI TTCGAA 1 cut(s) 2197
SgrBI CCGCGG 1 cut(s) 2221
SinI GGWCC 3 cut(s) 1504, 2829, 3179
SlaI CTCGAG 1 cut(s) 3440
SmaI CCCGGG 1 cut(s) 719
SmiMI CAYNNNNRTG 2 cut(s) 1567, 1944
SmlI CTYRAG 5 cut(s) 453, 2365, 2415, 2522, 3440
SmoI CTYRAG 5 cut(s) 453, 2365, 2415, 2522, 3440
SnaBI TACGTA 1 cut(s) 3226
SseBI AGGCCT 2 cut(s) 1454, 2811
SspI AATATT 2 cut(s) 1676, 2323
SspMI CTAG 5 cut(s) 603, 693, 1179, 1209, 3134
StuI AGGCCT 2 cut(s) 1454, 2811
StyD4I CCNGG 6 cut(s) 716, 717, 1271, 1424, 1582, 2380
StyI CCWWGG 2 cut(s) 111, 2085
TaiI ACGT 4 cut(s) 524, 1408, 2857, 3228
TaqI TCGA 5 cut(s) 749, 2197, 3269, 3441, 3490
TatI WGTACW 4 cut(s) 2250, 2467, 2861, 3196
TauI GCSGC 3 cut(s) 108, 1271, 3114
TfiI GAWTC 7 cut(s) 599, 1118, 1715, 1763, 1882, 2237, 2835
TseFI GTSAC 3 cut(s) 810, 2653, 2734
Tsp45I GTSAC 3 cut(s) 810, 2653, 2734
TspGWI ACGGA 2 cut(s) 571, 3201
TspMI CCCGGG 1 cut(s) 717
Van91I CCANNNNNTGG 1 cut(s) 3335
VneI GTGCAC 2 cut(s) 1527, 2127
VpaK11BI GGWCC 3 cut(s) 1504, 2829, 3179
VspI ATTAAT 2 cut(s) 992, 1830
XceI RCATGY 2 cut(s) 2283, 2952
XcmI CCANNNNNNNNNTGG 3 cut(s) 254, 1269, 1674
XhoI CTCGAG 1 cut(s) 3440
XmaI CCCGGG 1 cut(s) 717
XmiI GTMKAC 2 cut(s) 192, 3490
XmnI GAANNNNTTC 4 cut(s) 1173, 1842, 2605, 3162
XspI CTAG 5 cut(s) 603, 693, 1179, 1209, 3134
ZrmI AGTACT 1 cut(s) 2863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.