RLG00000010838

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006256
Physical Location & Seq
Reverse (-)
4701327 .. 4703507
2181 bp
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UTR
Exon/CDS
Intron
RLM00000010838

Sequence Viewer

Length: 444 bp
ATGCTGGTGAGTTCATTGTTTTCCAATTTGCGTGCTCAAGGGTTCAGTTCCTTGGGTATCAGGCAGTTTTTCAGGATGTGGAAGCTGGAGCAGTCAAATATTAAGAAAATGAAGGGGATCAATCGGGATGAAGCTTTTGCACTTCTTGATTCACTGAACGCAACTGCAAAGCAGTGTCCGGTACATCTTGCTGAAGTGGAACTCAAGCAATTCCAAACCCAACCTCCTCCACGCTACTCTGAGGCATTATTGGTTAAGAAATTAGAGGTGCTTGGGATTGGAAGTGCTTCGACATATGCAAGCACTATAAAGGATAGAAACTATTTGACAGTGAAAAGCTGTGTGTTACATCCAGAGTTTCTTGGTCGTATGGTGTCAGCATTTCTCTGCCACCATTTTTCTGAGGTCACAGCTTATAGCTTCACTGCTGGACATTGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

148

Amino Acids

16.49

Weight (kDa)

9.3

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_bac PF01131 45 - 143 1.8e-17 DNA topoisomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 213
AfaI GTAC 1 cut(s) 183
AluBI AGCT 5 cut(s) 85, 134, 339, 413, 420
AluI AGCT 5 cut(s) 85, 134, 339, 413, 420
Alw21I GWGCWC 1 cut(s) 37
AlwI GGATC 1 cut(s) 125
Asp700I GAANNNNTTC 1 cut(s) 286
AsuHPI GGTGA 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 107
BpuEI CTTGAG 2 cut(s) 21, 188
BsaJI CCNNGG 1 cut(s) 51
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 3 cut(s) 81, 133, 349
BseMII CTCAG 2 cut(s) 231, 393
BseRI GAGGAG 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 37
BsiSI CCGG 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 1 cut(s) 117
BspCNI CTCAG 2 cut(s) 232, 394
BspPI GGATC 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 1 cut(s) 117
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 33, 301
BstDEI CTNAG 2 cut(s) 240, 402
BstF5I GGATG 3 cut(s) 81, 133, 349
BstKTI GATC 1 cut(s) 120
BstMBI GATC 1 cut(s) 117
BtsCI GGATG 3 cut(s) 81, 133, 349
BtsI GCAGTG 2 cut(s) 179, 423
BtsIMutI CAGTG 4 cut(s) 152, 179, 336, 423
Cac8I GCNNGC 2 cut(s) 33, 301
Csp6I GTAC 1 cut(s) 182
CviJI RGCY 5 cut(s) 85, 134, 339, 413, 420
CviKI_1 RGCY 5 cut(s) 85, 134, 339, 413, 420
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 2 cut(s) 240, 402
DpnI GATC 1 cut(s) 119
DpnII GATC 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
FaiI YATR 5 cut(s) 295, 297, 308, 371, 417
FauNDI CATATG 1 cut(s) 295
FokI GGATG 3 cut(s) 88, 140, 336
GsuI CTGGAG 1 cut(s) 107
HapII CCGG 1 cut(s) 179
HindIII AAGCTT 1 cut(s) 132
HinfI GANTC 1 cut(s) 149
HpaII CCGG 1 cut(s) 179
HphI GGTGA 1 cut(s) 19
Hpy188I TCNGA 2 cut(s) 241, 403
Hpy188III TCNNGA 4 cut(s) 73, 125, 146, 353
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4V TGCA 3 cut(s) 140, 167, 299
HpyF3I CTNAG 2 cut(s) 240, 402
Kzo9I GATC 1 cut(s) 117
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 6 cut(s) 46, 58, 71, 192, 366, 414
MaeIII GTNAC 2 cut(s) 345, 406
MalI GATC 1 cut(s) 119
MboI GATC 1 cut(s) 117
MhlI GDGCHC 1 cut(s) 37
MluCI AATT 3 cut(s) 25, 209, 260
MnlI CCTC 5 cut(s) 234, 235, 237, 259, 397
MroXI GAANNNNTTC 1 cut(s) 286
MseI TTAA 2 cut(s) 102, 255
MspI CCGG 1 cut(s) 179
NdeI CATATG 1 cut(s) 295
NdeII GATC 1 cut(s) 117
NmuCI GTSAC 1 cut(s) 406
PdmI GAANNNNTTC 1 cut(s) 286
PfeI GAWTC 1 cut(s) 149
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SaqAI TTAA 2 cut(s) 102, 255
Sau3AI GATC 1 cut(s) 117
SduI GDGCHC 1 cut(s) 37
SetI ASST 8 cut(s) 87, 136, 226, 270, 341, 408, 415, 422
SmlI CTYRAG 2 cut(s) 36, 203
SmoI CTYRAG 2 cut(s) 36, 203
Sse9I AATT 3 cut(s) 25, 209, 260
SspI AATATT 1 cut(s) 100
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 331
TaqI TCGA 1 cut(s) 290
TasI AATT 3 cut(s) 25, 209, 260
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 2 cut(s) 102, 255
Tru9I TTAA 2 cut(s) 102, 255
TscAI CASTG 4 cut(s) 159, 179, 336, 430
TseFI GTSAC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 2 cut(s) 125, 144
TspRI CASTG 4 cut(s) 159, 179, 336, 430
XmnI GAANNNNTTC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.