AT4G31350

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004591
Physical Location & Seq
Forward (+)
15212429 .. 15216029
3601 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31350.4

Sequence Viewer

Length: 1131 bp
ATGTCAGAGTCTCGTCAAAGGCCTCCTTTTAAGGGCCCGAGATGGATTATCACTTTGGTTGTTCTGGTTACGGTTGTTGTCATTACCGCATTCATATATCCTCCGCGGAATTCTGTGGCTTGCTATATGTTTTCTGGTCCTGGATGTCCTTTATACCAGCAGTTTTTGTTTGTACCTACTCGGGAATTGACTGACTCTGAAGCTGCTGCTCAAGTTGTCATGAATGAAATCATGAACCTACCTCAATCTAAGACAGCAAATCCCAAGTTAGCTTTCATGTTCTTAACTCCAGGGACGCTGCCTTTTGAGCCTCTCTGGGAAATGTTTTTCCGTGGTCATGAGAACAAATTTTCAGTTTATGTGCATGCCTCAAAGAAAAGTCCTGTACATACAAGCAGCTATTTTGTTGGTCGAGATATTCACAGTCACAAGGTTGCTTGGGGTCAGATATCAATGGTTGATGCAGAAAGAAGGCTATTAGCTCATGCTCTCGTTGATCCTGATAATCAACACTTTATATTGCTGTCAGATAGTTGTGTACCGCTATTCGACTTTAACTATATCTATAACCACTTGATCTTCGCGAATCTCAGCTTCATTGATTGTTTTGAGGATCCTGGACCACATGGATCTGGTAGGTATTCACAACATATGCTGCCTGAGGTTGAAAAGAAGGATTTTCGGAAGGGCTCACAGTGGTTTTCAATGAAGAGGAGACATGCTATAGTCGTCATGGCGGACAGCCTTTACTACACAAAATTCAAGCTTTACTGTAGGCCAAATATGGAAGGACGCAACTGCTATGCGGACGAGCATTATTTTCCAACTCTTTTCAATATGATTGATCCAGATGGAATAGCAAACTGGTCGGTCACACATGTAGATTGGTCCGAGGGCAAATGGCATCCGAAGCTGTACAATGCAAGAGACATCACTCCTTACCTCATCAGGAAAATCAAGTCCATCCAACTTGCTTACCATGTCACAAGCGATTTAAAGAAAGTGACGACAGTAAAGCCGTGTCTATGGAAAGGAGAGCAACGTCCATGTTACCTATTTGCTCGGAAGTTTAATCCTGAAACTCTAGACCGATTGATGTATCTCTTCCCTAACTACACCTCGCTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

376

Amino Acids

43.75

Weight (kDa)

8.94

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 90 - 319 9.9e-85 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 106, 584
AciI CCGC 6 cut(s) 87, 104, 106, 542, 737, 806
AclWI GGATC 5 cut(s) 491, 608, 621, 637, 839
AcsI RAATTY 3 cut(s) 109, 347, 758
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 4 cut(s) 174, 387, 540, 917
AfiI CCNNNNNNNGG 1 cut(s) 32
AflIII ACRYGT 1 cut(s) 877
AgsI TTSAA 4 cut(s) 668, 705, 763, 835
AjnI CCWGG 3 cut(s) 139, 289, 616
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AluBI AGCT 7 cut(s) 203, 272, 399, 482, 594, 766, 913
AluI AGCT 7 cut(s) 203, 272, 399, 482, 594, 766, 913
Alw26I GTCTC 3 cut(s) 15, 709, 921
AlwI GGATC 5 cut(s) 491, 608, 621, 637, 839
Ama87I CYCGRG 2 cut(s) 37, 180
AoxI GGCC 3 cut(s) 20, 34, 776
ApaI GGGCCC 1 cut(s) 38
ApeKI GCWGC 5 cut(s) 203, 206, 298, 396, 655
ApoI RAATTY 3 cut(s) 109, 347, 758
AspS9I GGNCC 5 cut(s) 34, 35, 137, 620, 888
AvaI CYCGRG 2 cut(s) 37, 180
AvaII GGWCC 3 cut(s) 137, 620, 888
AxyI CCTNAGG 1 cut(s) 660
BaeGI GKGCMC 1 cut(s) 38
BamHI GGATCC 1 cut(s) 613
BanII GRGCYC 2 cut(s) 38, 692
BbvI GCAGC 5 cut(s) 190, 193, 285, 408, 642
BccI CCATC 3 cut(s) 36, 845, 971
BceAI ACGGC 1 cut(s) 1003
BcgI CGANNNNNNTGC 2 cut(s) 849, 883
BciT130I CCWGG 3 cut(s) 141, 291, 618
BcoDI GTCTC 3 cut(s) 15, 709, 921
BfaI CTAG 1 cut(s) 1085
BfmI CTRYAG 2 cut(s) 723, 772
BisI GCNGC 5 cut(s) 204, 207, 299, 397, 656
BlsI GCNGC 5 cut(s) 205, 208, 300, 398, 657
Bme1390I CCNGG 3 cut(s) 141, 291, 618
Bme18I GGWCC 3 cut(s) 137, 620, 888
BmeT110I CYCGRG 2 cut(s) 37, 180
BmgT120I GGNCC 5 cut(s) 34, 35, 137, 620, 888
BmiI GGNNCC 2 cut(s) 36, 615
BmrFI CCNGG 3 cut(s) 141, 291, 618
BmsI GCATC 2 cut(s) 451, 913
BpmI CTGGAG 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 195
BsaJI CCNNGG 4 cut(s) 104, 290, 331, 891
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 869
Bse21I CCTNAGG 1 cut(s) 660
BseBI CCWGG 3 cut(s) 141, 291, 618
BseDI CCNNGG 4 cut(s) 104, 290, 331, 891
BseGI GGATG 3 cut(s) 149, 904, 963
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 2 cut(s) 604, 651
BseNI ACTGG 1 cut(s) 869
BseRI GAGGAG 1 cut(s) 727
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 5 cut(s) 190, 193, 285, 408, 642
Bsh1236I CGCG 2 cut(s) 106, 584
BshFI GGCC 3 cut(s) 22, 36, 778
BsiHKCI CYCGRG 2 cut(s) 37, 180
BslFI GGGAC 1 cut(s) 307
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 3 cut(s) 15, 709, 921
BsmFI GGGAC 1 cut(s) 307
BsmI GAATGC 1 cut(s) 89
BsnI GGCC 3 cut(s) 22, 36, 778
BsoBI CYCGRG 2 cut(s) 37, 180
Bsp120I GGGCCC 1 cut(s) 34
Bsp1286I GDGCHC 2 cut(s) 38, 692
Bsp1407I TGTACA 2 cut(s) 385, 915
Bsp143I GATC 5 cut(s) 496, 576, 613, 629, 844
Bsp68I TCGCGA 1 cut(s) 584
BspACI CCGC 6 cut(s) 87, 104, 106, 542, 737, 806
BspANI GGCC 3 cut(s) 22, 36, 778
BspCNI CTCAG 2 cut(s) 603, 652
BspFNI CGCG 2 cut(s) 106, 584
BspHI TCATGA 3 cut(s) 219, 231, 337
BspLI GGNNCC 2 cut(s) 36, 615
BspPI GGATC 5 cut(s) 491, 608, 621, 637, 839
BsrGI TGTACA 2 cut(s) 385, 915
BsrI ACTGG 1 cut(s) 869
BssECI CCNNGG 4 cut(s) 104, 290, 331, 891
BssMI GATC 5 cut(s) 496, 576, 613, 629, 844
Bst2UI CCWGG 3 cut(s) 141, 291, 618
Bst4CI ACNGT 5 cut(s) 73, 425, 696, 773, 1012
Bst6I CTCTTC 2 cut(s) 704, 1109
BstAUI TGTACA 2 cut(s) 385, 915
BstC8I GCNNGC 2 cut(s) 121, 366
BstDEI CTNAG 3 cut(s) 249, 590, 660
BstDSI CCRYGG 2 cut(s) 104, 331
BstF5I GGATG 3 cut(s) 149, 904, 963
BstFNI CGCG 2 cut(s) 106, 584
BstKTI GATC 5 cut(s) 499, 579, 616, 632, 847
BstMAI GTCTC 3 cut(s) 15, 709, 921
BstMBI GATC 5 cut(s) 496, 576, 613, 629, 844
BstMWI GCNNNNNNNGC 2 cut(s) 307, 910
BstNI CCWGG 3 cut(s) 141, 291, 618
BstNSI RCATGY 3 cut(s) 368, 722, 881
BstSCI CCNGG 3 cut(s) 139, 289, 616
BstSFI CTRYAG 2 cut(s) 723, 772
BstSLI GKGCMC 1 cut(s) 38
BstUI CGCG 2 cut(s) 106, 584
BstV1I GCAGC 5 cut(s) 190, 193, 285, 408, 642
BstX2I RGATCY 2 cut(s) 613, 629
BstYI RGATCY 2 cut(s) 613, 629
Bsu36I CCTNAGG 1 cut(s) 660
BsuRI GGCC 3 cut(s) 22, 36, 778
BtgI CCRYGG 2 cut(s) 104, 331
BtsCI GGATG 3 cut(s) 149, 904, 963
BtsIMutI CAGTG 1 cut(s) 701
BtuMI TCGCGA 1 cut(s) 584
Cac8I GCNNGC 2 cut(s) 121, 366
CciI TCATGA 3 cut(s) 219, 231, 337
Cfr13I GGNCC 5 cut(s) 34, 35, 137, 620, 888
Cfr42I CCGCGG 1 cut(s) 107
CseI GACGC 2 cut(s) 304, 801
Csp6I GTAC 4 cut(s) 173, 386, 539, 916
CviQI GTAC 4 cut(s) 173, 386, 539, 916
DdeI CTNAG 3 cut(s) 249, 590, 660
DpnI GATC 5 cut(s) 498, 578, 615, 631, 846
DpnII GATC 5 cut(s) 496, 576, 613, 629, 844
DraI TTTAAA 1 cut(s) 996
Eam1104I CTCTTC 2 cut(s) 704, 1109
EarI CTCTTC 2 cut(s) 704, 1109
EciI GGCGGA 1 cut(s) 752
Eco147I AGGCCT 1 cut(s) 22
Eco24I GRGCYC 2 cut(s) 38, 692
Eco32I GATATC 1 cut(s) 450
Eco47I GGWCC 3 cut(s) 137, 620, 888
Eco57I CTGAAG 1 cut(s) 219
Eco81I CCTNAGG 1 cut(s) 660
Eco88I CYCGRG 2 cut(s) 37, 180
EcoO109I RGGNCCY 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 3 cut(s) 139, 289, 616
EcoRV GATATC 1 cut(s) 450
EcoT38I GRGCYC 2 cut(s) 38, 692
FalI AAGNNNNNCTT 2 cut(s) 10, 42
FaqI GGGAC 1 cut(s) 307
FauNDI CATATG 1 cut(s) 651
Fnu4HI GCNGC 5 cut(s) 204, 207, 299, 397, 656
FokI GGATG 3 cut(s) 156, 891, 950
FriOI GRGCYC 2 cut(s) 38, 692
Fsp4HI GCNGC 5 cut(s) 204, 207, 299, 397, 656
FspBI CTAG 1 cut(s) 1085
GluI GCNGC 5 cut(s) 204, 207, 299, 397, 656
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 3 cut(s) 22, 36, 778
HgaI GACGC 2 cut(s) 304, 801
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 3 cut(s) 8, 194, 586
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 8 cut(s) 7, 199, 447, 529, 684, 892, 909, 1065
Hpy8I GTNNAC 1 cut(s) 539
HpyAV CCTTC 4 cut(s) 465, 667, 679, 782
HpyCH4III ACNGT 5 cut(s) 73, 425, 696, 773, 1012
HpyCH4IV ACGT 1 cut(s) 1042
HpyCH4V TGCA 3 cut(s) 364, 464, 923
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 910
HpyF3I CTNAG 3 cut(s) 249, 590, 660
HpySE526I ACGT 1 cut(s) 1042
KspI CCGCGG 1 cut(s) 107
Kzo9I GATC 5 cut(s) 496, 576, 613, 629, 844
Lsp1109I GCAGC 5 cut(s) 190, 193, 285, 408, 642
LweI GCATC 2 cut(s) 451, 913
MaeI CTAG 1 cut(s) 1085
MaeII ACGT 1 cut(s) 1042
MaeIII GTNAC 6 cut(s) 67, 425, 871, 982, 1003, 1049
MalI GATC 5 cut(s) 498, 578, 615, 631, 846
MboI GATC 5 cut(s) 496, 576, 613, 629, 844
MboII GAAGA 3 cut(s) 571, 721, 1096
MflI RGATCY 2 cut(s) 613, 629
MhlI GDGCHC 2 cut(s) 38, 692
MluCI AATT 4 cut(s) 109, 185, 347, 758
MlyI GAGTC 2 cut(s) 17, 188
MmeI TCCRAC 2 cut(s) 848, 991
MseI TTAA 5 cut(s) 30, 284, 555, 995, 1071
MspA1I CMGCKG 1 cut(s) 106
MspR9I CCNGG 3 cut(s) 141, 291, 618
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 3 cut(s) 141, 291, 618
MvnI CGCG 2 cut(s) 106, 584
MwoI GCNNNNNNNGC 2 cut(s) 307, 910
NdeI CATATG 1 cut(s) 651
NdeII GATC 5 cut(s) 496, 576, 613, 629, 844
NlaIV GGNNCC 2 cut(s) 36, 615
NmuCI GTSAC 4 cut(s) 425, 871, 982, 1003
NruI TCGCGA 1 cut(s) 584
NspI RCATGY 3 cut(s) 368, 722, 881
PaeI GCATGC 1 cut(s) 368
PagI TCATGA 3 cut(s) 219, 231, 337
PceI AGGCCT 1 cut(s) 22
PciI ACATGT 1 cut(s) 877
PctI GAATGC 1 cut(s) 89
PfeI GAWTC 1 cut(s) 586
PfoI TCCNGGA 2 cut(s) 139, 616
PkrI GCNGC 5 cut(s) 205, 208, 300, 398, 657
PleI GAGTC 2 cut(s) 16, 188
PpsI GAGTC 2 cut(s) 16, 188
PscI ACATGT 1 cut(s) 877
Psp6I CCWGG 3 cut(s) 139, 289, 616
PspGI CCWGG 3 cut(s) 139, 289, 616
PspN4I GGNNCC 2 cut(s) 36, 615
PspOMI GGGCCC 1 cut(s) 34
PspPI GGNCC 5 cut(s) 34, 35, 137, 620, 888
PsuI RGATCY 2 cut(s) 613, 629
RruI TCGCGA 1 cut(s) 584
RsaI GTAC 4 cut(s) 174, 387, 540, 917
RsaNI GTAC 4 cut(s) 173, 386, 539, 916
SacII CCGCGG 1 cut(s) 107
SaqAI TTAA 5 cut(s) 30, 284, 555, 995, 1071
SatI GCNGC 5 cut(s) 204, 207, 299, 397, 656
Sau3AI GATC 5 cut(s) 496, 576, 613, 629, 844
Sau96I GGNCC 5 cut(s) 34, 35, 137, 620, 888
SchI GAGTC 2 cut(s) 17, 188
ScrFI CCNGG 3 cut(s) 141, 291, 618
SduI GDGCHC 2 cut(s) 38, 692
SfaNI GCATC 2 cut(s) 451, 913
SfcI CTRYAG 2 cut(s) 723, 772
Sfr303I CCGCGG 1 cut(s) 107
SgrBI CCGCGG 1 cut(s) 107
SinI GGWCC 3 cut(s) 137, 620, 888
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
SphI GCATGC 1 cut(s) 368
Sse9I AATT 4 cut(s) 109, 185, 347, 758
SseBI AGGCCT 1 cut(s) 22
SsiI CCGC 6 cut(s) 87, 104, 106, 542, 737, 806
SspMI CTAG 1 cut(s) 1085
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 3 cut(s) 139, 289, 616
TaaI ACNGT 5 cut(s) 73, 425, 696, 773, 1012
TaiI ACGT 1 cut(s) 1045
TaqI TCGA 2 cut(s) 412, 549
TaqII GACCGA 2 cut(s) 859, 1104
TasI AATT 4 cut(s) 109, 185, 347, 758
TatI WGTACW 2 cut(s) 385, 915
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 5 cut(s) 30, 284, 555, 995, 1071
Tru9I TTAA 5 cut(s) 30, 284, 555, 995, 1071
TscAI CASTG 1 cut(s) 701
TseFI GTSAC 4 cut(s) 425, 871, 982, 1003
TseI GCWGC 5 cut(s) 203, 206, 298, 396, 655
Tsp45I GTSAC 4 cut(s) 425, 871, 982, 1003
TspDTI ATGAA 7 cut(s) 82, 236, 240, 248, 265, 586, 722
TspGWI ACGGA 1 cut(s) 320
TspRI CASTG 1 cut(s) 701
VpaK11BI GGWCC 3 cut(s) 137, 620, 888
XapI RAATTY 3 cut(s) 109, 347, 758
XbaI TCTAGA 1 cut(s) 1084
XceI RCATGY 3 cut(s) 368, 722, 881
XspI CTAG 1 cut(s) 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.