MD00G1173000.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004591
Physical Location & Seq
Forward (+)
39958072 .. 39961852
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1173000.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGAAAAAGTCGTTCGCATGGCGGCCGCAACTGATGAGAGATACGGTGATGTCTAAGTCAAGGTCGCGTCCAGCGTTGAGAAGACCAACATGGATCATTGCATTGGTTTCGTTCATCTGTGTTTTTCTGATACTTGCCTACTTCTACTCTCCGAAAAACTCTGCCTCCTGCAATCTGTTTTCTTCTAGTGGCTGTCTGTTTGGAAGGACTCCCCCTAGCCCTGCGAGGGAGCTGACCGATGATGAGACTGCGTCGGAGGTTGTAGTTAGGGAGATTCTCAAGACGCCTCCTCTTCAATCCAAGAACCCGAAAATTGCTTTCATGTTTTTGACTCCCGGAACCTTGCCTTTCGAGGAGTTGTGGGAAAATTTCTTCCAAGGCCATGAAGACAAATTTACCGTTTATGTACATGCATCCAAGGAAAAACCAGTACATGCGAGCCGGTATTTTGCTGACAGAGACATACATAGTGAACAGGTCGTTTGGGGAAAATTTTCGATGGTTGAGGCAGAGAAGAGACTTTTGGCAAATGCACTTTTAGACCCCGACAACCAACATTTTGTCTTGCTGTCTGATAGTTGCATACCACTGCATAACTTCGATTATGTATATAACTATCTCATGTACACAAACGTCAGCTTTATCGATTGTTTTGTTGATCCTGGTCCGCATGGAACTGGCCGGTATTCAAAACATATGTTGCCTGAAGTTGACAGGAGCAATTTCCGGAAGGGTTCACAGATAGCACATGAGAAGCTGGATTACTTGCTTTGCAAATATGGTTCTTCGATTTCGGCAACACGCGCAATTGTCATGATGGCAGACTGTCTATACTATACAAAGTTCAAGCTCTTCTGCAGGCCAAACTTGGATGGACGCAACTGCTATTCGGATGAGCATTACTTGCCAACCTTCTTCAATCTGAAAGATCCTGGTGGACTCGCAAATTGGTCTGTAACATATGTCGATTGGTCTGAAGGGAAGTGGCATCCAAGAGCGTTCAGAACTCAGGATGTTACTTATGACCTTCTTAGGAACATCACGTCCATTGCTGAGAGCACGCATATTACAAGCGAAGACAAGAAGCGAATTCTGAACACTCCGTGCTTGTGGAACGGGATGAAGCGACCGTGTTATCTATTTGCCCGAAAGTTCTATCCAGAAACACTGGGGAAACTGGTTCAGCTTTTCTCCAATTACACAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

404

Amino Acids

46.68

Weight (kDa)

8.88

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 105 - 347 4.8e-69 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 67, 804
AccIII TCCGGA 1 cut(s) 726
AciI CCGC 3 cut(s) 22, 26, 668
AclWI GGATC 3 cut(s) 101, 653, 923
AcoI YGGCCR 2 cut(s) 23, 679
AcsI RAATTY 4 cut(s) 367, 392, 491, 1089
AcuI CTGAAG 2 cut(s) 726, 996
AcyI GRCGYC 1 cut(s) 284
AdeI CACNNNGTG 1 cut(s) 1104
AfaI GTAC 3 cut(s) 408, 432, 626
AfiI CCNNNNNNNGG 1 cut(s) 226
AgsI TTSAA 4 cut(s) 296, 690, 847, 919
AjiI CACGTC 1 cut(s) 1044
AjnI CCWGG 2 cut(s) 661, 931
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AloI GAACNNNNNNTCC 2 cut(s) 1028, 1060
AluBI AGCT 5 cut(s) 232, 639, 757, 850, 1186
AluI AGCT 5 cut(s) 232, 639, 757, 850, 1186
Alw21I GWGCWC 1 cut(s) 1061
Alw26I GTCTC 3 cut(s) 239, 453, 511
AlwI GGATC 3 cut(s) 101, 653, 923
Aor13HI TCCGGA 1 cut(s) 726
AoxI GGCC 4 cut(s) 23, 379, 679, 860
ApoI RAATTY 4 cut(s) 367, 392, 491, 1089
Asp700I GAANNNNTTC 1 cut(s) 493
AspLEI GCGC 1 cut(s) 806
AspS9I GGNCC 1 cut(s) 665
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 1 cut(s) 58
AvaII GGWCC 1 cut(s) 665
BarI GAAGNNNNNNTAC 2 cut(s) 746, 778
BbsI GAAGAC 3 cut(s) 88, 393, 1083
Bbv12I GWGCWC 1 cut(s) 1061
BccI CCATC 3 cut(s) 493, 811, 866
BcgI CGANNNNNNTGC 2 cut(s) 90, 124
BciT130I CCWGG 2 cut(s) 663, 933
BcnI CCSGG 1 cut(s) 336
BcoDI GTCTC 3 cut(s) 239, 453, 511
BfaI CTAG 2 cut(s) 186, 216
BfmI CTRYAG 1 cut(s) 856
BisI GCNGC 2 cut(s) 23, 26
BlsI GCNGC 2 cut(s) 24, 27
Bme1390I CCNGG 3 cut(s) 336, 663, 933
Bme18I GGWCC 1 cut(s) 665
BmgBI CACGTC 1 cut(s) 1044
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 3 cut(s) 336, 663, 933
BmrI ACTGGG 1 cut(s) 1178
BmsI GCATC 2 cut(s) 422, 997
BmuI ACTGGG 1 cut(s) 1178
BpiI GAAGAC 3 cut(s) 88, 393, 1083
BpuEI CTTGAG 1 cut(s) 263
BpuMI CCSGG 1 cut(s) 336
Bsa29I ATCGAT 1 cut(s) 645
BsaHI GRCGYC 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 376, 417
BsaWI WCCGGW 1 cut(s) 726
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse118I RCCGGY 2 cut(s) 441, 681
Bse1I ACTGG 4 cut(s) 428, 682, 1173, 1182
Bse3DI GCAATG 2 cut(s) 96, 1047
BseAI TCCGGA 1 cut(s) 726
BseBI CCWGG 2 cut(s) 663, 933
BseCI ATCGAT 1 cut(s) 645
BseDI CCNNGG 2 cut(s) 376, 417
BseGI GGATG 6 cut(s) 413, 877, 898, 988, 1018, 1125
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMI GCAATG 2 cut(s) 96, 1047
BseMII CTCAG 2 cut(s) 1022, 1044
BseNI ACTGG 4 cut(s) 428, 682, 1173, 1182
BseRI GAGGAG 2 cut(s) 279, 368
BseX3I CGGCCG 1 cut(s) 23
Bsh1236I CGCG 2 cut(s) 67, 804
Bsh1285I CGRYCG 2 cut(s) 26, 1130
BshFI GGCC 4 cut(s) 25, 381, 681, 862
BshVI ATCGAT 1 cut(s) 645
BsiEI CGRYCG 2 cut(s) 26, 1130
BsiHKAI GWGCWC 1 cut(s) 1061
BsiSI CCGG 4 cut(s) 336, 442, 682, 727
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 3 cut(s) 239, 453, 511
BsnI GGCC 4 cut(s) 25, 381, 681, 862
Bsp1286I GDGCHC 1 cut(s) 1061
Bsp13I TCCGGA 1 cut(s) 726
Bsp1407I TGTACA 2 cut(s) 406, 624
Bsp143I GATC 3 cut(s) 93, 658, 928
BspACI CCGC 3 cut(s) 22, 26, 668
BspANI GGCC 4 cut(s) 25, 381, 681, 862
BspCNI CTCAG 2 cut(s) 1021, 1045
BspDI ATCGAT 1 cut(s) 645
BspEI TCCGGA 1 cut(s) 726
BspFNI CGCG 2 cut(s) 67, 804
BspHI TCATGA 1 cut(s) 813
BspLI GGNNCC 1 cut(s) 340
BspMAI CTGCAG 1 cut(s) 860
BspPI GGATC 3 cut(s) 101, 653, 923
BspQI GCTCTTC 1 cut(s) 857
BsrDI GCAATG 2 cut(s) 96, 1047
BsrFI RCCGGY 2 cut(s) 441, 681
BsrGI TGTACA 2 cut(s) 406, 624
BsrI ACTGG 4 cut(s) 428, 682, 1173, 1182
BssAI RCCGGY 2 cut(s) 441, 681
BssECI CCNNGG 2 cut(s) 376, 417
BssMI GATC 3 cut(s) 93, 658, 928
BssNI GRCGYC 1 cut(s) 284
BssT1I CCWWGG 2 cut(s) 376, 417
Bst2UI CCWGG 2 cut(s) 663, 933
Bst4CI ACNGT 4 cut(s) 46, 400, 827, 1131
Bst6I CTCTTC 3 cut(s) 297, 509, 857
BstACI GRCGYC 1 cut(s) 284
BstAPI GCANNNNNTGC 1 cut(s) 904
BstAUI TGTACA 2 cut(s) 406, 624
BstC8I GCNNGC 3 cut(s) 439, 860, 1061
BstDEI CTNAG 4 cut(s) 54, 1008, 1031, 1053
BstF5I GGATG 6 cut(s) 413, 877, 898, 988, 1018, 1125
BstFNI CGCG 2 cut(s) 67, 804
BstHHI GCGC 1 cut(s) 806
BstKTI GATC 3 cut(s) 96, 661, 931
BstMAI GTCTC 3 cut(s) 239, 453, 511
BstMBI GATC 3 cut(s) 93, 658, 928
BstMCI CGRYCG 2 cut(s) 26, 1130
BstMWI GCNNNNNNNGC 2 cut(s) 803, 904
BstNI CCWGG 2 cut(s) 663, 933
BstNSI RCATGY 2 cut(s) 413, 437
BstSCI CCNGG 3 cut(s) 334, 661, 931
BstSFI CTRYAG 1 cut(s) 856
BstUI CGCG 2 cut(s) 67, 804
BstV2I GAAGAC 3 cut(s) 88, 393, 1083
BstX2I RGATCY 1 cut(s) 928
BstYI RGATCY 1 cut(s) 928
BstZI CGGCCG 1 cut(s) 23
Bsu15I ATCGAT 1 cut(s) 645
BsuRI GGCC 4 cut(s) 25, 381, 681, 862
BsuTUI ATCGAT 1 cut(s) 645
BtrI CACGTC 1 cut(s) 1044
BtsCI GGATG 6 cut(s) 413, 877, 898, 988, 1018, 1125
BtsI GCAGTG 1 cut(s) 587
BtsIMutI CAGTG 2 cut(s) 587, 1166
Cac8I GCNNGC 3 cut(s) 439, 860, 1061
CciI TCATGA 1 cut(s) 813
CciNI GCGGCCGC 1 cut(s) 23
CfoI GCGC 1 cut(s) 806
Cfr10I RCCGGY 2 cut(s) 441, 681
Cfr13I GGNCC 1 cut(s) 665
ClaI ATCGAT 1 cut(s) 645
CseI GACGC 4 cut(s) 56, 240, 292, 885
Csp6I GTAC 3 cut(s) 407, 431, 625
CviQI GTAC 3 cut(s) 407, 431, 625
DdeI CTNAG 4 cut(s) 54, 1008, 1031, 1053
DpnI GATC 3 cut(s) 95, 660, 930
DpnII GATC 3 cut(s) 93, 658, 928
DraIII CACNNNGTG 1 cut(s) 1104
EaeI YGGCCR 2 cut(s) 23, 679
EagI CGGCCG 1 cut(s) 23
Eam1104I CTCTTC 3 cut(s) 297, 509, 857
EarI CTCTTC 3 cut(s) 297, 509, 857
EclXI CGGCCG 1 cut(s) 23
Eco130I CCWWGG 2 cut(s) 376, 417
Eco47I GGWCC 1 cut(s) 665
Eco52I CGGCCG 1 cut(s) 23
Eco57I CTGAAG 2 cut(s) 726, 996
EcoRI GAATTC 1 cut(s) 1089
EcoRII CCWGG 2 cut(s) 661, 931
EcoT14I CCWWGG 2 cut(s) 376, 417
EcoT22I ATGCAT 1 cut(s) 415
ErhI CCWWGG 2 cut(s) 376, 417
FauNDI CATATG 2 cut(s) 696, 961
Fnu4HI GCNGC 2 cut(s) 23, 26
FokI GGATG 6 cut(s) 400, 884, 905, 975, 1025, 1132
Fsp4HI GCNGC 2 cut(s) 23, 26
FspBI CTAG 2 cut(s) 186, 216
GlaI GCGC 1 cut(s) 805
GluI GCNGC 2 cut(s) 23, 26
HaeIII GGCC 4 cut(s) 25, 381, 681, 862
HapII CCGG 4 cut(s) 336, 442, 682, 727
HgaI GACGC 4 cut(s) 56, 240, 292, 885
HhaI GCGC 1 cut(s) 806
Hin1I GRCGYC 1 cut(s) 284
Hin6I GCGC 1 cut(s) 804
HinP1I GCGC 1 cut(s) 804
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HinfI GANTC 4 cut(s) 208, 274, 331, 939
HpaII CCGG 4 cut(s) 336, 442, 682, 727
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 5 cut(s) 473, 627, 712, 737, 938
Hpy188I TCNGA 9 cut(s) 129, 153, 256, 574, 892, 924, 976, 1004, 1095
Hpy188III TCNNGA 5 cut(s) 280, 727, 814, 1010, 1160
Hpy8I GTNNAC 5 cut(s) 473, 627, 712, 737, 938
Hpy99I CGWCG 1 cut(s) 256
HpyAV CCTTC 5 cut(s) 198, 724, 922, 971, 1037
HpyCH4III ACNGT 4 cut(s) 46, 400, 827, 1131
HpyCH4IV ACGT 2 cut(s) 633, 1043
HpyCH4V TGCA 8 cut(s) 101, 171, 413, 533, 582, 592, 774, 858
HpyF10VI GCNNNNNNNGC 2 cut(s) 803, 904
HpyF3I CTNAG 4 cut(s) 54, 1008, 1031, 1053
HpySE526I ACGT 2 cut(s) 633, 1043
Hsp92I GRCGYC 1 cut(s) 284
HspAI GCGC 1 cut(s) 804
Kpn2I TCCGGA 1 cut(s) 726
Kzo9I GATC 3 cut(s) 93, 658, 928
LguI GCTCTTC 1 cut(s) 857
LmnI GCTCC 2 cut(s) 229, 717
LweI GCATC 2 cut(s) 422, 997
MaeI CTAG 2 cut(s) 186, 216
MaeII ACGT 2 cut(s) 633, 1043
MaeIII GTNAC 2 cut(s) 955, 1015
MalI GATC 3 cut(s) 95, 660, 930
MboI GATC 3 cut(s) 93, 658, 928
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 1 cut(s) 928
MhlI GDGCHC 1 cut(s) 1061
MluCI AATT 9 cut(s) 312, 367, 392, 491, 721, 807, 946, 1089, 1195
MlyI GAGTC 3 cut(s) 202, 325, 933
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 7 cut(s) 175, 219, 250, 297, 300, 346, 499
Mph1103I ATGCAT 1 cut(s) 415
MroI TCCGGA 1 cut(s) 726
MroXI GAANNNNTTC 1 cut(s) 493
MspI CCGG 4 cut(s) 336, 442, 682, 727
MspR9I CCNGG 3 cut(s) 336, 663, 933
MunI CAATTG 1 cut(s) 807
MvaI CCWGG 2 cut(s) 663, 933
MvnI CGCG 2 cut(s) 67, 804
MwoI GCNNNNNNNGC 2 cut(s) 803, 904
NciI CCSGG 1 cut(s) 336
NdeI CATATG 2 cut(s) 696, 961
NdeII GATC 3 cut(s) 93, 658, 928
NlaIV GGNNCC 1 cut(s) 340
NotI GCGGCCGC 1 cut(s) 23
NsiI ATGCAT 1 cut(s) 415
NspI RCATGY 2 cut(s) 413, 437
PagI TCATGA 1 cut(s) 813
PciSI GCTCTTC 1 cut(s) 857
PcsI WCGNNNNNNNCGW 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 493
PfeI GAWTC 1 cut(s) 274
PflFI GACNNNGTC 1 cut(s) 250
PfoI TCCNGGA 1 cut(s) 334
PkrI GCNGC 2 cut(s) 24, 27
PleI GAGTC 3 cut(s) 202, 325, 933
PpsI GAGTC 3 cut(s) 202, 325, 933
Psp6I CCWGG 2 cut(s) 661, 931
PspGI CCWGG 2 cut(s) 661, 931
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 665
PstI CTGCAG 1 cut(s) 860
PsuI RGATCY 1 cut(s) 928
PsyI GACNNNGTC 1 cut(s) 250
RsaI GTAC 3 cut(s) 408, 432, 626
RsaNI GTAC 3 cut(s) 407, 431, 625
SapI GCTCTTC 1 cut(s) 857
SatI GCNGC 2 cut(s) 23, 26
Sau3AI GATC 3 cut(s) 93, 658, 928
Sau96I GGNCC 1 cut(s) 665
SchI GAGTC 3 cut(s) 202, 325, 933
ScrFI CCNGG 3 cut(s) 336, 663, 933
SduI GDGCHC 1 cut(s) 1061
SfaNI GCATC 2 cut(s) 422, 997
SfcI CTRYAG 1 cut(s) 856
SinI GGWCC 1 cut(s) 665
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 9 cut(s) 312, 367, 392, 491, 721, 807, 946, 1089, 1195
SsiI CCGC 3 cut(s) 22, 26, 668
SspMI CTAG 2 cut(s) 186, 216
StyD4I CCNGG 3 cut(s) 334, 661, 931
StyI CCWWGG 2 cut(s) 376, 417
TaaI ACNGT 4 cut(s) 46, 400, 827, 1131
TaiI ACGT 2 cut(s) 636, 1046
TaqI TCGA 6 cut(s) 351, 497, 600, 645, 788, 966
TaqII GACCGA 1 cut(s) 251
TasI AATT 9 cut(s) 312, 367, 392, 491, 721, 807, 946, 1089, 1195
TatI WGTACW 3 cut(s) 406, 430, 624
TauI GCSGC 2 cut(s) 25, 28
TfiI GAWTC 1 cut(s) 274
TscAI CASTG 2 cut(s) 594, 1173
TspDTI ATGAA 5 cut(s) 17, 103, 310, 399, 1136
TspGWI ACGGA 1 cut(s) 1092
TspRI CASTG 2 cut(s) 594, 1173
Tth111I GACNNNGTC 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 665
XapI RAATTY 4 cut(s) 367, 392, 491, 1089
XceI RCATGY 2 cut(s) 413, 437
XmnI GAANNNNTTC 1 cut(s) 493
XspI CTAG 2 cut(s) 186, 216
Zsp2I ATGCAT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.