Rorug06G0393700

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004591
Physical Location & Seq
Forward (+)
53944197 .. 53948803
4607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0393700.1

Sequence Viewer

Length: 2619 bp
ATGTTGGGAGGTTCAATGGCAATGTGTGGCCAAGAGCTGGAGCAAATGTCCAGTGTCGATCAGGGCAGTCAGTATAGCCTCTCCGATGGCATTTTGCCTTCTCTTGGTGCTGCCTCTCGTAACCACCGCAGACCGAAACTACGTCGTTTCATCATCTCCCCCTTCAATCCCCATTACAAGTTTTGGCAGTCGTTTCTCATATTGTTGGTGTTCTACACGGCATGGGTGTGTCCCTTTGAATTTGGGTTCCTGGACAGTCCAAAAGGGCCTCTGGCTATTACTGATAATGTTGTCAATGCCTTTTTTGGCATTGATATTATCTTGACGTTCTTTGTGGCTTTCCTTGATAAAACTTCTTATTTGCTCATTGATGCCCCAAAGCTAATTGCTCTGAGATATGCCAAGACCTGGTTGGCCTTTGACATCATCTCCATTATCCCTTCAGAATTTGCTCAAGCGGTTTTGCCTCATCCTCTTGCGAATTATGGATACTTCAATATTCTTCGGCTATGGCGTCTCCGAAGAGTCAGTGGAATGTTTGCCAGATTGGAAAAAGATCGGAATTATAGCTACTTCTGGGTTCGATGCTGCAAGCTTATCTTTGTTACTCTGTTTACAGTTCATTGTGGGGGCTGCATCTTCTACTTCCTTGCTGCGAATTATCGTGATCCTAAAAGGACATGGCTTGGGCTTATCTCAGATAACTTTAACGATATAAGTTTGTGGAACCGGTATGTGACATCAATGTACTGGTCCATAATCACGCTCACGACCACCGGCTATGGTGATTTGCATCCTGTAAATTCTCAAGAAATGATTTTCGATATCTTCTATATGCTCTTTAATGTTGGGCTGCAAGCTTACCTGATTGGAAACATGACCAACTTAATCGTCCATGGGACTGCTCGAACTAGACAATTTAGAGATACCATTCACGCTGCCTCGAGTTTTGCTTTAAGGAACCAAATACCTGATCGTCTGCATGAACAGATGCTTGCGCATTTGTGTTTGAAGTACAGAACTAATTCAGAAGGGCTGCAGCAGCAAGAGACTCTTGATGCACTCCCTCACGCCATCAGATCAAGTATCTCACACTATCTCTTCAATTCACTTGTTGACAGTGTTTACCTTTTTCAGGGGGTATCGAGAGACATGCTTTTCCAACTGGTCGCGGAGATGAAGGCCGAGTATTTTCCTCCGAAAGAAGATGTCATCTTGCAGAATGAAGCGCCCACTGACATGTATATAGTGGTTACTGGTTCTCTGGAACTTATATTCCAAAAGAATGGAGCAGAACTGGTATTTAGTGAAGCAAAGACAGGAGATGTTTTTGGTGCCATAGGTGTGCTGTGTTACAGGCCACAACTGTTCACAGTTCGGACGAAGGGACTCTGTCAGCTGCTGCGTTTGAATAGGACAGCTTTCTTGAATATTGTTCAGGCAAATGTTGGAGATGGGACAATTATCATGAGCAATTTTCTTCAGCATTTGAGAGAAATGAAGGATCCCATAATGGAAGGAATTCTAACAGATGTAGAACATATGCTGGCTAGGGGAAAGATGGACATGCCTCTTAGTTTAGGGTTTGCTGCAAAGAGGAGTGATGATTTGTTGCTACATCAATTGCTAAGACGGGGTGCAGATCCGAATGAGACAGATCATACAGGCCGGACAGCTATGCACATTGCAGCATCAAATGGAAGTCAGCATTGTGCGACTTTACTTCTAGAGTATGGAGGTGATCCTAACATCAAAGATTGTGAAGAAAATGTTCCTCTATGGGAAGCAATAATGGGCAAACATGAATCTTTGATTAAACTTCTCTTAGAAAATGGAGCTGAGATTAGTTCTGGCAATGTGGGCCATTATGCTTGTGCTGCTGTTGAGCAAAACAACTTAGAGCTGCTCAAAGACATTGTCCGATATGGTGGTGATGTTACATTGCCCAAGACCGATGGGACTACAGCACTCCATACAGCAGTGAGTGAAGGAAATGCTGAGATAGTTAAGTTCCTCTTAGAACAAGGAGCTGATGCTGACAATAGGGATAGCTATGGATGGAGTCCGAGGGCTTTGGCAGAGCATCAGGGACATGAAGAAATAATGGAGCTATTTCAGCACAAATCTGGAGTTAAGAAACCAACTACTGTTCCTCCGCCAAACGATCCTCAGCTACCACGCCTTGTGAAGTTCCAGAGTGAGCCCATTATACCACCCTATGTCCGGGATGGCAGGCCAAACTCAGAAACACGGTGGTTGGATGATACTCGGATAAGAAGGTTCAACAACTTTCCCAATTCGCTTTTTGGGATCATGTCAGCTGCCAATACCGGTGAGAGAGACAAAGACCTCATCAAACCTGCAGCCAATTTTGTCCGTGGTAGCCGTAGTAAGAGCAAATATCCAGCCAGAGTTACTCTTAGTTGCCCAGAAAAAAGTGGAACTGCTGCAAAGCTTGTGGTTGTGCCAGAGTCCCTTCAACAATTACTTGACATCGGTGCCAAAAAATTTCAGTTTTGTCCCACTAAAGTTTTAACCACAGAAGGAGCTGAAGTTGAAGATATAGAGCTTGTTAGGGATGGTGATCATCTTGTTCTTGTTGGTGATGGTGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

872

Amino Acids

98.15

Weight (kDa)

6.26

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 59 - 304 8.5e-43 Ion transport protein
Ion_trans_2 PF07885 206 - 297 8.8e-12 Ion channel
cNMP_binding PF00027 397 - 482 1.8e-14 Cyclic nucleotide-binding domain
Ank_2 PF12796 528 - 617 7.2e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 530 - 576 2.1e-06 Ankyrin repeats (many copies)
Ank PF00023 556 - 586 1.7e-06 Ankyrin repeat
Ank_2 PF12796 626 - 708 7.4e-14 Ankyrin repeats (3 copies)
Ank_5 PF13857 645 - 693 5.2e-09 Ankyrin repeats (many copies)
Ank PF00023 652 - 683 9.9e-08 Ankyrin repeat
KHA PF11834 804 - 866 3.3e-21 KHA, dimerisation domain of potassium ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 999
Acc36I ACCTGC 1 cut(s) 2368
AccB1I GGYRCC 2 cut(s) 1334, 2498
AccB7I CCANNNNNTGG 2 cut(s) 37, 408
AccII CGCG 1 cut(s) 1172
AciI CCGC 4 cut(s) 127, 458, 1172, 2156
AclWI GGATC 7 cut(s) 662, 1499, 1512, 1637, 1736, 2159, 2318
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 5 cut(s) 239, 446, 802, 1521, 2507
AcuI CTGAAG 3 cut(s) 426, 1466, 2571
AcyI GRCGYC 1 cut(s) 514
AfaI GTAC 2 cut(s) 749, 1016
AfiI CCNNNNNNNGG 5 cut(s) 37, 104, 408, 1135, 2223
AflIII ACRYGT 1 cut(s) 1239
AgeI ACCGGT 2 cut(s) 729, 2330
AjnI CCWGG 2 cut(s) 249, 407
AloI GAACNNNNNNTCC 2 cut(s) 1260, 1292
Alw26I GTCTC 5 cut(s) 521, 1043, 1143, 1646, 2334
AlwI GGATC 7 cut(s) 662, 1499, 1512, 1637, 1736, 2159, 2318
AlwNI CAGNNNCTG 1 cut(s) 1402
Ama87I CYCGRG 1 cut(s) 943
AoxI GGCC 8 cut(s) 28, 266, 414, 1182, 1358, 1666, 1861, 2234
ApoI RAATTY 5 cut(s) 239, 446, 802, 1521, 2507
ArsI GACNNNNNNTTYG 2 cut(s) 1902, 1934
AsiGI ACCGGT 2 cut(s) 729, 2330
Asp700I GAANNNNTTC 3 cut(s) 1024, 1521, 1770
AspLEI GCGC 2 cut(s) 1000, 1231
AspS9I GGNCC 3 cut(s) 266, 753, 1861
AsuC2I CCSGG 1 cut(s) 2225
AsuHPI GGTGA 6 cut(s) 797, 1751, 1943, 2345, 2594, 2615
AvaI CYCGRG 1 cut(s) 943
AvaII GGWCC 1 cut(s) 753
BalI TGGCCA 1 cut(s) 30
BamHI GGATCC 1 cut(s) 1504
BanI GGYRCC 2 cut(s) 1334, 2498
BanII GRGCYC 1 cut(s) 2205
BbvCI CCTCAGC 1 cut(s) 2169
BccI CCATC 9 cut(s) 80, 1082, 1448, 1555, 1949, 2052, 2222, 2573, 2600
BceAI ACGGC 2 cut(s) 234, 2370
BcgI CGANNNNNNTGC 2 cut(s) 1135, 1169
BciT130I CCWGG 2 cut(s) 251, 409
BciVI GTATCC 1 cut(s) 482
BclI TGATCA 1 cut(s) 2584
BcnI CCSGG 1 cut(s) 2225
BcoDI GTCTC 5 cut(s) 521, 1043, 1143, 1646, 2334
BfaI CTAG 3 cut(s) 912, 1551, 1727
BfmI CTRYAG 3 cut(s) 1037, 1962, 2361
BfoI RGCGCY 1 cut(s) 1232
BfuAI ACCTGC 1 cut(s) 2368
BfuI GTATCC 1 cut(s) 482
Bme1390I CCNGG 3 cut(s) 251, 409, 2225
Bme18I GGWCC 1 cut(s) 753
BmeT110I CYCGRG 1 cut(s) 943
BmgT120I GGNCC 3 cut(s) 266, 753, 1861
BmiI GGNNCC 6 cut(s) 248, 728, 962, 1336, 1506, 2500
BmrFI CCNGG 3 cut(s) 251, 409, 2225
BmsI GCATC 9 cut(s) 361, 575, 645, 802, 981, 1048, 1700, 2023, 2092
BpmI CTGGAG 2 cut(s) 59, 2148
Bpu10I CCTNAGC 1 cut(s) 2169
BpuEI CTTGAG 2 cut(s) 438, 792
BpuMI CCSGG 1 cut(s) 2225
BsaBI GATNNNNATC 2 cut(s) 792, 2583
BsaHI GRCGYC 1 cut(s) 514
BsaJI CCNNGG 3 cut(s) 895, 2066, 2377
BsaWI WCCGGW 2 cut(s) 729, 2330
BsaXI ACNNNNNCTCC 8 cut(s) 32, 62, 413, 443, 2137, 2167, 2538, 2568
Bsc4I CCNNNNNNNGG 5 cut(s) 37, 104, 408, 1135, 2223
Bse118I RCCGGY 3 cut(s) 729, 776, 2330
Bse1I ACTGG 5 cut(s) 51, 755, 1170, 1261, 1302
Bse3DI GCAATG 4 cut(s) 27, 1683, 1861, 1940
Bse8I GATNNNNATC 2 cut(s) 792, 2583
BseBI CCWGG 2 cut(s) 251, 409
BseDI CCNNGG 3 cut(s) 895, 2066, 2377
BseGI GGATG 6 cut(s) 469, 793, 2063, 2233, 2266, 2584
BseJI GATNNNNATC 2 cut(s) 792, 2583
BseLI CCNNNNNNNGG 5 cut(s) 37, 104, 408, 1135, 2223
BseMI GCAATG 4 cut(s) 27, 1683, 1861, 1940
BseMII CTCAG 6 cut(s) 383, 711, 1830, 1989, 2183, 2256
BseNI ACTGG 5 cut(s) 51, 755, 1170, 1261, 1302
BseRI GAGGAG 1 cut(s) 1612
BsgI GTGCAG 1 cut(s) 1659
Bsh1236I CGCG 1 cut(s) 1172
BshFI GGCC 8 cut(s) 30, 268, 416, 1184, 1360, 1668, 1863, 2236
BshNI GGYRCC 2 cut(s) 1334, 2498
BshTI ACCGGT 2 cut(s) 729, 2330
BsiHKCI CYCGRG 1 cut(s) 943
BsiSI CCGG 5 cut(s) 730, 777, 1669, 2224, 2331
BslFI GGGAC 8 cut(s) 216, 913, 1401, 1471, 1972, 2103, 2458, 2505
BslI CCNNNNNNNGG 5 cut(s) 37, 104, 408, 1135, 2223
BsmAI GTCTC 5 cut(s) 521, 1043, 1143, 1646, 2334
BsmBI CGTCTC 1 cut(s) 521
BsmFI GGGAC 8 cut(s) 216, 913, 1401, 1471, 1972, 2103, 2458, 2505
BsnI GGCC 8 cut(s) 30, 268, 416, 1184, 1360, 1668, 1863, 2236
BsoBI CYCGRG 1 cut(s) 943
Bsp1286I GDGCHC 1 cut(s) 2205
Bsp19I CCATGG 1 cut(s) 895
BspACI CCGC 4 cut(s) 127, 458, 1172, 2156
BspANI GGCC 8 cut(s) 30, 268, 416, 1184, 1360, 1668, 1863, 2236
BspCNI CTCAG 6 cut(s) 384, 710, 1831, 1990, 2182, 2255
BspFNI CGCG 1 cut(s) 1172
BspHI TCATGA 1 cut(s) 1467
BspLI GGNNCC 6 cut(s) 248, 728, 962, 1336, 1506, 2500
BspMAI CTGCAG 2 cut(s) 1041, 2365
BspMI ACCTGC 1 cut(s) 2368
BspPI GGATC 7 cut(s) 662, 1499, 1512, 1637, 1736, 2159, 2318
BspT107I GGYRCC 2 cut(s) 1334, 2498
BsrDI GCAATG 4 cut(s) 27, 1683, 1861, 1940
BsrFI RCCGGY 3 cut(s) 729, 776, 2330
BsrI ACTGG 5 cut(s) 51, 755, 1170, 1261, 1302
BssAI RCCGGY 3 cut(s) 729, 776, 2330
BssECI CCNNGG 3 cut(s) 895, 2066, 2377
BssNI GRCGYC 1 cut(s) 514
BssT1I CCWWGG 1 cut(s) 895
Bst2UI CCWGG 2 cut(s) 251, 409
Bst4CI ACNGT 7 cut(s) 257, 619, 1121, 1368, 1375, 2149, 2253
Bst6I CTCTTC 2 cut(s) 517, 1106
BstACI GRCGYC 1 cut(s) 514
BstC8I GCNNGC 5 cut(s) 593, 858, 996, 1548, 2234
BstDSI CCRYGG 2 cut(s) 895, 2377
BstENI CCTNNNNNAGG 1 cut(s) 1133
BstF5I GGATG 6 cut(s) 469, 793, 2063, 2233, 2266, 2584
BstFNI CGCG 1 cut(s) 1172
BstH2I RGCGCY 1 cut(s) 1232
BstHHI GCGC 2 cut(s) 1000, 1231
BstMAI GTCTC 5 cut(s) 521, 1043, 1143, 1646, 2334
BstMWI GCNNNNNNNGC 4 cut(s) 1042, 1860, 1877, 2116
BstNI CCWGG 2 cut(s) 251, 409
BstNSI RCATGY 3 cut(s) 1156, 1243, 1570
BstSCI CCNGG 3 cut(s) 249, 407, 2223
BstSFI CTRYAG 3 cut(s) 1037, 1962, 2361
BstUI CGCG 1 cut(s) 1172
BstX2I RGATCY 2 cut(s) 1504, 1642
BstXI CCANNNNNNTGG 1 cut(s) 1286
BstYI RGATCY 2 cut(s) 1504, 1642
BsuI GTATCC 1 cut(s) 482
BsuRI GGCC 8 cut(s) 30, 268, 416, 1184, 1360, 1668, 1863, 2236
BtgI CCRYGG 2 cut(s) 895, 2377
BtsCI GGATG 6 cut(s) 469, 793, 2063, 2233, 2266, 2584
BtsI GCAGTG 1 cut(s) 1986
BtsIMutI CAGTG 5 cut(s) 58, 535, 1126, 1233, 1986
BveI ACCTGC 1 cut(s) 2368
Cac8I GCNNGC 5 cut(s) 593, 858, 996, 1548, 2234
CaiI CAGNNNCTG 1 cut(s) 1402
CciI TCATGA 1 cut(s) 1467
CfoI GCGC 2 cut(s) 1000, 1231
Cfr10I RCCGGY 3 cut(s) 729, 776, 2330
Cfr13I GGNCC 3 cut(s) 266, 753, 1861
CseI GACGC 1 cut(s) 503
CsiI ACCWGGT 1 cut(s) 407
Csp6I GTAC 2 cut(s) 748, 1015
CspAI ACCGGT 2 cut(s) 729, 2330
CspCI CAANNNNNGTGG 2 cut(s) 2439, 2474
CviQI GTAC 2 cut(s) 748, 1015
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 517, 1106
EarI CTCTTC 2 cut(s) 517, 1106
EciI GGCGGA 1 cut(s) 2145
Eco130I CCWWGG 1 cut(s) 895
Eco24I GRGCYC 1 cut(s) 2205
Eco32I GATATC 1 cut(s) 826
Eco47I GGWCC 1 cut(s) 753
Eco57I CTGAAG 3 cut(s) 426, 1466, 2571
Eco88I CYCGRG 1 cut(s) 943
EcoNI CCTNNNNNAGG 1 cut(s) 1133
EcoO109I RGGNCCY 1 cut(s) 266
EcoRI GAATTC 1 cut(s) 1521
EcoRII CCWGG 2 cut(s) 249, 407
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 1 cut(s) 895
EcoT38I GRGCYC 1 cut(s) 2205
ErhI CCWWGG 1 cut(s) 895
Esp3I CGTCTC 1 cut(s) 521
FalI AAGNNNNNCTT 6 cut(s) 584, 616, 1038, 1070, 2000, 2032
FaqI GGGAC 8 cut(s) 216, 913, 1401, 1471, 1972, 2103, 2458, 2505
FauNDI CATATG 1 cut(s) 1542
FbaI TGATCA 1 cut(s) 2584
FokI GGATG 6 cut(s) 456, 780, 2070, 2240, 2273, 2591
FriOI GRGCYC 1 cut(s) 2205
FspBI CTAG 3 cut(s) 912, 1551, 1727
FspI TGCGCA 1 cut(s) 999
GlaI GCGC 2 cut(s) 999, 1230
GsuI CTGGAG 2 cut(s) 59, 2148
HaeII RGCGCY 1 cut(s) 1232
HaeIII GGCC 8 cut(s) 30, 268, 416, 1184, 1360, 1668, 1863, 2236
HapII CCGG 5 cut(s) 730, 777, 1669, 2224, 2331
HgaI GACGC 1 cut(s) 503
HhaI GCGC 2 cut(s) 1000, 1231
Hin1I GRCGYC 1 cut(s) 514
Hin6I GCGC 2 cut(s) 998, 1229
HinP1I GCGC 2 cut(s) 998, 1229
HincII GTYRAC 1 cut(s) 1117
HindII GTYRAC 1 cut(s) 1117
HindIII AAGCTT 3 cut(s) 593, 858, 2453
HinfI GANTC 6 cut(s) 525, 1051, 1389, 1805, 2062, 2471
HpaII CCGG 5 cut(s) 730, 777, 1669, 2224, 2331
HphI GGTGA 6 cut(s) 797, 1751, 1943, 2345, 2594, 2615
Hpy166II GTNNAC 4 cut(s) 615, 1117, 1126, 1371
Hpy8I GTNNAC 4 cut(s) 615, 1117, 1126, 1371
Hpy99I CGWCG 1 cut(s) 147
HpyCH4III ACNGT 7 cut(s) 257, 619, 1121, 1368, 1375, 2149, 2253
HpyCH4IV ACGT 2 cut(s) 142, 326
HpyF10VI GCNNNNNNNGC 4 cut(s) 1042, 1860, 1877, 2116
HpySE526I ACGT 2 cut(s) 142, 326
Hsp92I GRCGYC 1 cut(s) 514
HspAI GCGC 2 cut(s) 998, 1229
Ksp22I TGATCA 1 cut(s) 2584
LmnI GCTCC 6 cut(s) 40, 1289, 1835, 2027, 2107, 2546
LweI GCATC 9 cut(s) 361, 575, 645, 802, 981, 1048, 1700, 2023, 2092
MabI ACCWGGT 1 cut(s) 407
MaeI CTAG 3 cut(s) 912, 1551, 1727
MaeII ACGT 2 cut(s) 142, 326
MaeIII GTNAC 7 cut(s) 119, 604, 736, 1252, 1352, 1936, 2413
MfeI CAATTG 1 cut(s) 1622
MflI RGATCY 2 cut(s) 1504, 1642
MhlI GDGCHC 1 cut(s) 2205
MlsI TGGCCA 1 cut(s) 30
MluNI TGGCCA 1 cut(s) 30
MlyI GAGTC 5 cut(s) 534, 1045, 1383, 2071, 2480
MmeI TCCRAC 3 cut(s) 1186, 1429, 2238
Mox20I TGGCCA 1 cut(s) 30
MroXI GAANNNNTTC 3 cut(s) 1024, 1521, 1770
MscI TGGCCA 1 cut(s) 30
MseI TTAA 8 cut(s) 708, 843, 887, 956, 1815, 2007, 2133, 2534
MslI CAYNNNNRTG 3 cut(s) 226, 1238, 1343
Msp20I TGGCCA 1 cut(s) 30
MspA1I CMGCKG 2 cut(s) 1399, 2321
MspI CCGG 5 cut(s) 730, 777, 1669, 2224, 2331
MspR9I CCNGG 3 cut(s) 251, 409, 2225
MunI CAATTG 1 cut(s) 1622
MvaI CCWGG 2 cut(s) 251, 409
MvnI CGCG 1 cut(s) 1172
MwoI GCNNNNNNNGC 4 cut(s) 1042, 1860, 1877, 2116
NciI CCSGG 1 cut(s) 2225
NcoI CCATGG 1 cut(s) 895
NdeI CATATG 1 cut(s) 1542
NlaIV GGNNCC 6 cut(s) 248, 728, 962, 1336, 1506, 2500
NmeAIII GCCGAG 1 cut(s) 1210
NmuCI GTSAC 1 cut(s) 736
NsbI TGCGCA 1 cut(s) 999
NspI RCATGY 3 cut(s) 1156, 1243, 1570
PaeR7I CTCGAG 1 cut(s) 943
PagI TCATGA 1 cut(s) 1467
PciI ACATGT 1 cut(s) 1239
PcsI WCGNNNNNNNCGW 1 cut(s) 511
PdmI GAANNNNTTC 3 cut(s) 1024, 1521, 1770
PfeI GAWTC 1 cut(s) 1805
PflFI GACNNNGTC 2 cut(s) 1392, 1916
PflMI CCANNNNNTGG 2 cut(s) 37, 408
PfoI TCCNGGA 2 cut(s) 249, 2223
PinAI ACCGGT 2 cut(s) 729, 2330
PleI GAGTC 5 cut(s) 533, 1045, 1383, 2070, 2479
PpsI GAGTC 5 cut(s) 533, 1045, 1383, 2070, 2479
PscI ACATGT 1 cut(s) 1239
Psp6I CCWGG 2 cut(s) 249, 407
PspGI CCWGG 2 cut(s) 249, 407
PspN4I GGNNCC 6 cut(s) 248, 728, 962, 1336, 1506, 2500
PspPI GGNCC 3 cut(s) 266, 753, 1861
PspXI VCTCGAGB 1 cut(s) 943
PstI CTGCAG 2 cut(s) 1041, 2365
PstNI CAGNNNCTG 1 cut(s) 1402
PsuI RGATCY 2 cut(s) 1504, 1642
PsyI GACNNNGTC 2 cut(s) 1392, 1916
PvuII CAGCTG 2 cut(s) 1399, 2321
RsaI GTAC 2 cut(s) 749, 1016
RsaNI GTAC 2 cut(s) 748, 1015
RseI CAYNNNNRTG 3 cut(s) 226, 1238, 1343
SaqAI TTAA 8 cut(s) 708, 843, 887, 956, 1815, 2007, 2133, 2534
Sau96I GGNCC 3 cut(s) 266, 753, 1861
SchI GAGTC 5 cut(s) 534, 1045, 1383, 2071, 2480
ScrFI CCNGG 3 cut(s) 251, 409, 2225
SduI GDGCHC 1 cut(s) 2205
SexAI ACCWGGT 1 cut(s) 407
SfaNI GCATC 9 cut(s) 361, 575, 645, 802, 981, 1048, 1700, 2023, 2092
SfcI CTRYAG 3 cut(s) 1037, 1962, 2361
Sfr274I CTCGAG 1 cut(s) 943
SinI GGWCC 1 cut(s) 753
SlaI CTCGAG 1 cut(s) 943
SmiMI CAYNNNNRTG 3 cut(s) 226, 1238, 1343
SmlI CTYRAG 3 cut(s) 453, 807, 943
SmoI CTYRAG 3 cut(s) 453, 807, 943
SsiI CCGC 4 cut(s) 127, 458, 1172, 2156
SspI AATATT 2 cut(s) 499, 1432
SspMI CTAG 3 cut(s) 912, 1551, 1727
StyD4I CCNGG 3 cut(s) 249, 407, 2223
StyI CCWWGG 1 cut(s) 895
TaaI ACNGT 7 cut(s) 257, 619, 1121, 1368, 1375, 2149, 2253
TaiI ACGT 2 cut(s) 145, 329
TaqI TCGA 6 cut(s) 57, 583, 822, 907, 944, 1145
TaqII GACCGA 2 cut(s) 148, 1967
TatI WGTACW 2 cut(s) 747, 1014
TfiI GAWTC 1 cut(s) 1805
Tru1I TTAA 8 cut(s) 708, 843, 887, 956, 1815, 2007, 2133, 2534
Tru9I TTAA 8 cut(s) 708, 843, 887, 956, 1815, 2007, 2133, 2534
TscAI CASTG 5 cut(s) 58, 535, 1126, 1240, 1986
TseFI GTSAC 1 cut(s) 736
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 8 cut(s) 139, 611, 999, 1193, 1239, 1514, 1818, 2109
TspGWI ACGGA 1 cut(s) 2366
TspRI CASTG 5 cut(s) 58, 535, 1126, 1240, 1986
Tth111I GACNNNGTC 2 cut(s) 1392, 1916
Van91I CCANNNNNTGG 2 cut(s) 37, 408
VpaK11BI GGWCC 1 cut(s) 753
XagI CCTNNNNNAGG 1 cut(s) 1133
XapI RAATTY 5 cut(s) 239, 446, 802, 1521, 2507
XbaI TCTAGA 1 cut(s) 1726
XceI RCATGY 3 cut(s) 1156, 1243, 1570
XcmI CCANNNNNNNNNTGG 1 cut(s) 409
XhoI CTCGAG 1 cut(s) 943
XmnI GAANNNNTTC 3 cut(s) 1024, 1521, 1770
XspI CTAG 3 cut(s) 912, 1551, 1727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.