Rroxscaffold_7G00156970

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004591
Physical Location & Seq
Forward (+)
832572 .. 835966
3395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00156970.1

Sequence Viewer

Length: 597 bp
ATGTACACGAATGTCAGCTTTATAGACTGTTTTGTGGATCCCGGTCCACATGGAACTGGACGGTATGCTGAGCAAATGATGCCCGAAGTTGAAAAGCACGAATTTCGAAAGGGTTCACAGTGGTTCACTATGAAGAGGCAACATGCTATTGTCATGATGGCAGACAGTCTATACTATACCAAGTTCAAGCTTCACTGCAGGCCGAACATGGACGGGCGCAACTGCTATTCGGACGAGCATTACCTGCCAACCTTCTTTCGGATGAAAGATCCTGCTGGTCTTGCAAATTGGTCGGTAACATTTGTTGATTGGTCTGAAGGGAAATGGCATCCAAGAGCATTTAGGACTCAGGATGTCACTTATGATCTTCTTAGGAACATCACGCGGGTTTATACACCTTCTGTGTCAGATGCTTGCATCGTGAGACAAAAGGTGTATACGCCCCTTATGCGTTGTGGAGCTCATGAGTCTACTAGTACAGTACTGACAAAGCAGAAGAGAGCAATACCGGCCCAGAAGCAGTCATCAGCGAATACCTACGAGTTTTTTAAGGTGGGAAATTTTGGGACAAGCGGGGTCTGTACCCTCCATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

198

Amino Acids

22.95

Weight (kDa)

9.12

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 4 - 127 1.2e-37 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 252
AccI GTMKAC 2 cut(s) 437, 470
AccII CGCG 1 cut(s) 385
AciI CCGC 2 cut(s) 385, 573
AclWI GGATC 3 cut(s) 32, 45, 263
AcsI RAATTY 2 cut(s) 101, 559
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 4 cut(s) 5, 478, 483, 583
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 2 cut(s) 92, 187
AhlI ACTAGT 1 cut(s) 473
AluBI AGCT 3 cut(s) 18, 190, 461
AluI AGCT 3 cut(s) 18, 190, 461
Alw21I GWGCWC 1 cut(s) 463
Alw26I GTCTC 1 cut(s) 418
AlwI GGATC 3 cut(s) 32, 45, 263
AoxI GGCC 2 cut(s) 200, 510
ApoI RAATTY 2 cut(s) 101, 559
Asp700I GAANNNNTTC 1 cut(s) 112
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 2 cut(s) 44, 511
AsuC2I CCSGG 1 cut(s) 42
AsuII TTCGAA 1 cut(s) 106
AvaII GGWCC 1 cut(s) 44
BamHI GGATCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 463
Bbv12I GWGCWC 1 cut(s) 463
BccI CCATC 1 cut(s) 151
BcgI CGANNNNNNTGC 4 cut(s) 86, 120, 273, 307
BcnI CCSGG 1 cut(s) 42
BcoDI GTCTC 1 cut(s) 418
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 1 cut(s) 474
BfmI CTRYAG 1 cut(s) 196
BfuAI ACCTGC 1 cut(s) 252
BlpI GCTNAGC 1 cut(s) 69
BmcAI AGTACT 1 cut(s) 483
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 44, 511
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 4 cut(s) 69, 337, 400, 426
Bpu1102I GCTNAGC 1 cut(s) 69
Bpu14I TTCGAA 1 cut(s) 106
BpuMI CCSGG 1 cut(s) 42
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse118I RCCGGY 1 cut(s) 508
Bse1I ACTGG 1 cut(s) 61
BseGI GGATG 3 cut(s) 267, 328, 358
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 60, 362
BseNI ACTGG 1 cut(s) 61
Bsh1236I CGCG 1 cut(s) 385
BshFI GGCC 2 cut(s) 202, 512
BsiHKAI GWGCWC 1 cut(s) 463
BsiSI CCGG 2 cut(s) 42, 509
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 1 cut(s) 418
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 2 cut(s) 202, 512
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 1 cut(s) 463
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 37, 268, 364
Bsp1720I GCTNAGC 1 cut(s) 69
BspACI CCGC 2 cut(s) 385, 573
BspANI GGCC 2 cut(s) 202, 512
BspCNI CTCAG 2 cut(s) 61, 361
BspFNI CGCG 1 cut(s) 385
BspHI TCATGA 2 cut(s) 153, 463
BspLI GGNNCC 1 cut(s) 39
BspMAI CTGCAG 1 cut(s) 200
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 3 cut(s) 32, 45, 263
BspT104I TTCGAA 1 cut(s) 106
BsrFI RCCGGY 1 cut(s) 508
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 61
BssAI RCCGGY 1 cut(s) 508
BssMI GATC 3 cut(s) 37, 268, 364
BssNAI GTATAC 1 cut(s) 438
Bst1107I GTATAC 1 cut(s) 438
Bst4CI ACNGT 5 cut(s) 29, 63, 120, 167, 481
Bst6I CTCTTC 2 cut(s) 128, 491
BstAPI GCANNNNNTGC 2 cut(s) 79, 244
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 106
BstC8I GCNNGC 2 cut(s) 200, 415
BstDEI CTNAG 3 cut(s) 69, 348, 371
BstF5I GGATG 3 cut(s) 267, 328, 358
BstFNI CGCG 1 cut(s) 385
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 3 cut(s) 40, 271, 367
BstMAI GTCTC 1 cut(s) 418
BstMBI GATC 3 cut(s) 37, 268, 364
BstMWI GCNNNNNNNGC 5 cut(s) 79, 244, 281, 448, 509
BstNSI RCATGY 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 40
BstSFI CTRYAG 1 cut(s) 196
BstUI CGCG 1 cut(s) 385
BstX2I RGATCY 2 cut(s) 37, 268
BstYI RGATCY 2 cut(s) 37, 268
BstZ17I GTATAC 1 cut(s) 438
BsuRI GGCC 2 cut(s) 202, 512
BtsCI GGATG 3 cut(s) 267, 328, 358
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 2 cut(s) 125, 193
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 2 cut(s) 200, 415
CciI TCATGA 2 cut(s) 153, 463
CfoI GCGC 1 cut(s) 219
Cfr10I RCCGGY 1 cut(s) 508
Cfr13I GGNCC 2 cut(s) 44, 511
Csp6I GTAC 4 cut(s) 4, 477, 482, 582
CviAII CATG 5 cut(s) 50, 143, 154, 208, 464
CviJI RGCY 5 cut(s) 18, 190, 202, 461, 512
CviKI_1 RGCY 5 cut(s) 18, 190, 202, 461, 512
CviQI GTAC 4 cut(s) 4, 477, 482, 582
DdeI CTNAG 3 cut(s) 69, 348, 371
DpnI GATC 3 cut(s) 39, 270, 366
DpnII GATC 3 cut(s) 37, 268, 364
Eam1104I CTCTTC 2 cut(s) 128, 491
EarI CTCTTC 2 cut(s) 128, 491
Ecl136II GAGCTC 1 cut(s) 461
Eco24I GRGCYC 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 44
Eco53kI GAGCTC 1 cut(s) 461
Eco57I CTGAAG 1 cut(s) 336
EcoICRI GAGCTC 1 cut(s) 461
EcoT38I GRGCYC 1 cut(s) 463
FaeI CATG 5 cut(s) 53, 146, 157, 211, 467
FaqI GGGAC 1 cut(s) 580
FatI CATG 5 cut(s) 49, 142, 153, 207, 463
FauI CCCGC 2 cut(s) 378, 566
FblI GTMKAC 2 cut(s) 437, 470
FokI GGATG 3 cut(s) 274, 315, 365
FriOI GRGCYC 1 cut(s) 463
FspBI CTAG 1 cut(s) 474
GlaI GCGC 1 cut(s) 218
HaeIII GGCC 2 cut(s) 202, 512
HapII CCGG 2 cut(s) 42, 509
HhaI GCGC 1 cut(s) 219
Hin1II CATG 5 cut(s) 53, 146, 157, 211, 467
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 2 cut(s) 346, 467
HpaII CCGG 2 cut(s) 42, 509
Hpy166II GTNNAC 6 cut(s) 6, 47, 116, 126, 438, 471
Hpy188I TCNGA 4 cut(s) 232, 261, 316, 409
Hpy188III TCNNGA 4 cut(s) 154, 350, 421, 464
Hpy8I GTNNAC 6 cut(s) 6, 47, 116, 126, 438, 471
HpyAV CCTTC 3 cut(s) 262, 311, 408
HpyCH4III ACNGT 5 cut(s) 29, 63, 120, 167, 481
HpyCH4V TGCA 3 cut(s) 198, 284, 417
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 244, 281, 448, 509
HpyF3I CTNAG 3 cut(s) 69, 348, 371
Hsp92II CATG 5 cut(s) 53, 146, 157, 211, 467
HspAI GCGC 1 cut(s) 217
Kzo9I GATC 3 cut(s) 37, 268, 364
LmnI GCTCC 1 cut(s) 458
LpnPI CCDG 9 cut(s) 42, 55, 184, 257, 261, 285, 335, 522, 527
LweI GCATC 4 cut(s) 69, 337, 400, 426
MaeI CTAG 1 cut(s) 474
MaeIII GTNAC 2 cut(s) 295, 355
MalI GATC 3 cut(s) 39, 270, 366
MboI GATC 3 cut(s) 37, 268, 364
MboII GAAGA 3 cut(s) 145, 359, 508
MflI RGATCY 2 cut(s) 37, 268
MhlI GDGCHC 1 cut(s) 463
MluCI AATT 3 cut(s) 101, 286, 559
MlyI GAGTC 2 cut(s) 340, 476
MnlI CCTC 2 cut(s) 129, 596
MroXI GAANNNNTTC 1 cut(s) 112
MseI TTAA 1 cut(s) 549
MspI CCGG 2 cut(s) 42, 509
MspR9I CCNGG 1 cut(s) 42
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 5 cut(s) 79, 244, 281, 448, 509
NciI CCSGG 1 cut(s) 42
NdeII GATC 3 cut(s) 37, 268, 364
NlaIII CATG 5 cut(s) 53, 146, 157, 211, 467
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 1 cut(s) 355
NspI RCATGY 1 cut(s) 146
NspV TTCGAA 1 cut(s) 106
PagI TCATGA 2 cut(s) 153, 463
PdmI GAANNNNTTC 1 cut(s) 112
PleI GAGTC 2 cut(s) 340, 475
PpsI GAGTC 2 cut(s) 340, 475
Psp124BI GAGCTC 1 cut(s) 463
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 44, 511
PstI CTGCAG 1 cut(s) 200
PsuI RGATCY 2 cut(s) 37, 268
RsaI GTAC 4 cut(s) 5, 478, 483, 583
RsaNI GTAC 4 cut(s) 4, 477, 482, 582
SacI GAGCTC 1 cut(s) 463
SaqAI TTAA 1 cut(s) 549
Sau3AI GATC 3 cut(s) 37, 268, 364
Sau96I GGNCC 2 cut(s) 44, 511
ScaI AGTACT 1 cut(s) 483
SchI GAGTC 2 cut(s) 340, 476
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 1 cut(s) 463
SetI ASST 9 cut(s) 20, 192, 246, 254, 400, 435, 463, 539, 555
SfaNI GCATC 4 cut(s) 69, 337, 400, 426
SfcI CTRYAG 1 cut(s) 196
SfuI TTCGAA 1 cut(s) 106
SinI GGWCC 1 cut(s) 44
SpeI ACTAGT 1 cut(s) 473
Sse9I AATT 3 cut(s) 101, 286, 559
SsiI CCGC 2 cut(s) 385, 573
SspMI CTAG 1 cut(s) 474
SstI GAGCTC 1 cut(s) 463
StyD4I CCNGG 1 cut(s) 40
TaaI ACNGT 5 cut(s) 29, 63, 120, 167, 481
TaqI TCGA 1 cut(s) 106
TasI AATT 3 cut(s) 101, 286, 559
TatI WGTACW 3 cut(s) 3, 476, 481
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 2 cut(s) 125, 200
TseFI GTSAC 1 cut(s) 355
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 2 cut(s) 146, 278
TspRI CASTG 2 cut(s) 125, 200
VpaK11BI GGWCC 1 cut(s) 44
XapI RAATTY 2 cut(s) 101, 559
XceI RCATGY 1 cut(s) 146
XmiI GTMKAC 2 cut(s) 437, 470
XmnI GAANNNNTTC 1 cut(s) 112
XspI CTAG 1 cut(s) 474
ZrmI AGTACT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.