Rw6G044420

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004591
Physical Location & Seq
Reverse (-)
67587103 .. 67590323
3221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G044420.1

Sequence Viewer

Length: 1224 bp
ATGTTGCCTTCAACCCAATTGAAAAAAGGTGGCGCATGGCGGCCACAATTGTTCAAAGACATTATGGTCATGTCCAAGTCAAGGTCTCGTTCACATATGAAAAGACCAAGATGGATTATTGCATTGGTTTGTATTCTCTGCGTTTTTCTGATTACTGCTTATTTCTATCCGTCGGAAAGCTCTACGACATGCTATCTCTTCTCATCTCCTAATTGTGATGGGATATTTGGAAAGACTCCACCTAGTCCTGCAAGGGAACTAACTGACGATGAGACTGCATCTTCAGTTGTAGTTAGGGAAATTCTTAATACGCCTCCTCTTCAGTCGAAGAACCCGAAAATCGCTTTCATGTTTTTGACTCCGGGCACGTTACCTTTCGAGGAGTTATGGGAAAATTTCTTTCAAGGCCATGAGGACAAATTCAGTGTTTATGTACATGCATCTAAGCAGAAACCAATACATGTGAGCCGCTATTTTGTTGATAGAGACATACATAGTGAACAGATGCCTTTGATAATAAATTATCAGGTAGTTTGGGGAAAATTTTCAATGGTTGAGGCAGAAAAGAGACTCTTGGCCAATGCACTTTTAGACCCTGATAACCAACACTTTGTTCTGCTGTCTGACAGCTGCATACCACTGCATAGCTTTGATTATGTATATAATTATCTCATGTACACGAATGTCAGCTTTATAGACTGTTTTGTGGATCCCGGTCCACATGGAACTGGGCGGTATGCTGAGCAAATGATGCCCGAAGTTGAAAAGCACGAATTTCGAAAGGGTTCACAGTGGTTCACTATGAAGAGGCAACATGCTATTGTCATGATGGCAGACAGTCTATACTATACCAAGTTCAAGCTTCACTGCAGGCCGAACATGGACGGCCGCAACTGCTATTCGGACGAGCATTACCTGCCAACCTTCTTTCGGATGAAAGATCCTGCTGGTCTTGCAAATTGGTCGGTAACATTTGTTGATTGGTCTGAAGGGAAATGGCATCCAAGAGCATTTAGGACTCAGGATGTCACTTATGATCTTCTTAGGAACATCACGTCTATCGCTGATAGCACACATGTGACAAGTGATGCAACGAAAACAGTATTGAGGACTCCTTGCCTGTGGAATGGGATGAAGCGACCCTGTTTCTTATTTGCCAGAAAGTTCTATCCTGAAACTCTGGGGAAACTGATACACCTTTTCTCCAATTATACGAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

407

Amino Acids

47.35

Weight (kDa)

8.76

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 114 - 351 4.5e-77 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 924
AciI CCGC 4 cut(s) 40, 469, 733, 889
AclWI GGATC 3 cut(s) 704, 717, 935
AcoI YGGCCR 3 cut(s) 41, 576, 886
AcsI RAATTY 5 cut(s) 300, 394, 419, 542, 773
AcuI CTGAAG 3 cut(s) 267, 305, 1008
AfaI GTAC 2 cut(s) 435, 677
AfiI CCNNNNNNNGG 2 cut(s) 81, 930
AflIII ACRYGT 2 cut(s) 460, 1075
AgsI TTSAA 7 cut(s) 12, 22, 55, 404, 549, 764, 859
AjiI CACGTC 1 cut(s) 1056
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AleI CACNNNNGTG 1 cut(s) 1076
AluBI AGCT 5 cut(s) 180, 630, 648, 690, 862
AluI AGCT 5 cut(s) 180, 630, 648, 690, 862
Alw26I GTCTC 4 cut(s) 90, 266, 480, 562
AlwI GGATC 3 cut(s) 704, 717, 935
AoxI GGCC 5 cut(s) 41, 406, 576, 872, 886
ApeKI GCWGC 1 cut(s) 630
ApoI RAATTY 5 cut(s) 300, 394, 419, 542, 773
Asp700I GAANNNNTTC 2 cut(s) 544, 784
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 1 cut(s) 716
AsuC2I CCSGG 2 cut(s) 363, 714
AsuII TTCGAA 1 cut(s) 778
AvaII GGWCC 1 cut(s) 716
BaeGI GKGCMC 1 cut(s) 368
BalI TGGCCA 1 cut(s) 578
BamHI GGATCC 1 cut(s) 709
BbvI GCAGC 1 cut(s) 617
BccI CCATC 3 cut(s) 105, 212, 823
BceAI ACGGC 1 cut(s) 901
BcgI CGANNNNNNTGC 6 cut(s) 257, 291, 758, 792, 945, 979
BcnI CCSGG 2 cut(s) 363, 714
BcoDI GTCTC 4 cut(s) 90, 266, 480, 562
BfaI CTAG 1 cut(s) 243
BfmI CTRYAG 1 cut(s) 868
BfuAI ACCTGC 1 cut(s) 924
BisI GCNGC 4 cut(s) 41, 469, 631, 889
BlpI GCTNAGC 1 cut(s) 741
BlsI GCNGC 4 cut(s) 42, 470, 632, 890
Bme1390I CCNGG 2 cut(s) 363, 714
Bme18I GGWCC 1 cut(s) 716
BmgBI CACGTC 1 cut(s) 1056
BmgT120I GGNCC 1 cut(s) 716
BmiI GGNNCC 1 cut(s) 711
BmrFI CCNGG 2 cut(s) 363, 714
BmrI ACTGGG 1 cut(s) 738
BmsI GCATC 6 cut(s) 287, 449, 495, 741, 1009, 1078
BmuI ACTGGG 1 cut(s) 738
Bpu1102I GCTNAGC 1 cut(s) 741
Bpu14I TTCGAA 1 cut(s) 778
BpuMI CCSGG 2 cut(s) 363, 714
BsaI GGTCTC 1 cut(s) 90
BsaXI ACNNNNNCTCC 2 cut(s) 1187, 1217
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 930
Bse1I ACTGG 1 cut(s) 733
BseGI GGATG 4 cut(s) 939, 1000, 1030, 1137
BseLI CCNNNNNNNGG 2 cut(s) 81, 930
BseMII CTCAG 2 cut(s) 732, 1034
BseNI ACTGG 1 cut(s) 733
BseRI GAGGAG 2 cut(s) 306, 395
BseSI GKGCMC 1 cut(s) 368
BseX3I CGGCCG 1 cut(s) 886
BseXI GCAGC 1 cut(s) 617
Bsh1285I CGRYCG 1 cut(s) 889
BshFI GGCC 5 cut(s) 43, 408, 578, 874, 888
BsiEI CGRYCG 1 cut(s) 889
BsiSI CCGG 2 cut(s) 362, 714
BslI CCNNNNNNNGG 2 cut(s) 81, 930
BsmAI GTCTC 4 cut(s) 90, 266, 480, 562
BsnI GGCC 5 cut(s) 43, 408, 578, 874, 888
Bso31I GGTCTC 1 cut(s) 90
Bsp119I TTCGAA 1 cut(s) 778
Bsp1286I GDGCHC 1 cut(s) 368
Bsp1407I TGTACA 2 cut(s) 433, 675
Bsp143I GATC 3 cut(s) 709, 940, 1036
Bsp1720I GCTNAGC 1 cut(s) 741
BspACI CCGC 4 cut(s) 40, 469, 733, 889
BspANI GGCC 5 cut(s) 43, 408, 578, 874, 888
BspCNI CTCAG 2 cut(s) 733, 1033
BspHI TCATGA 1 cut(s) 825
BspLI GGNNCC 1 cut(s) 711
BspMAI CTGCAG 1 cut(s) 872
BspMI ACCTGC 1 cut(s) 924
BspPI GGATC 3 cut(s) 704, 717, 935
BspT104I TTCGAA 1 cut(s) 778
BspTNI GGTCTC 1 cut(s) 90
BsrGI TGTACA 2 cut(s) 433, 675
BsrI ACTGG 1 cut(s) 733
BssMI GATC 3 cut(s) 709, 940, 1036
Bst4CI ACNGT 4 cut(s) 701, 792, 839, 1102
Bst6I CTCTTC 3 cut(s) 203, 324, 800
BstAPI GCANNNNNTGC 2 cut(s) 751, 916
BstAUI TGTACA 2 cut(s) 433, 675
BstBI TTCGAA 1 cut(s) 778
BstC8I GCNNGC 1 cut(s) 872
BstDEI CTNAG 4 cut(s) 444, 741, 1020, 1043
BstF5I GGATG 4 cut(s) 939, 1000, 1030, 1137
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 3 cut(s) 712, 943, 1039
BstMAI GTCTC 4 cut(s) 90, 266, 480, 562
BstMBI GATC 3 cut(s) 709, 940, 1036
BstMCI CGRYCG 1 cut(s) 889
BstMWI GCNNNNNNNGC 4 cut(s) 751, 894, 916, 953
BstNSI RCATGY 5 cut(s) 192, 440, 464, 818, 1079
BstSCI CCNGG 2 cut(s) 361, 712
BstSFI CTRYAG 1 cut(s) 868
BstSLI GKGCMC 1 cut(s) 368
BstV1I GCAGC 1 cut(s) 617
BstX2I RGATCY 2 cut(s) 709, 940
BstYI RGATCY 2 cut(s) 709, 940
BstZI CGGCCG 1 cut(s) 886
BsuRI GGCC 5 cut(s) 43, 408, 578, 874, 888
BtrI CACGTC 1 cut(s) 1056
BtsCI GGATG 4 cut(s) 939, 1000, 1030, 1137
BtsI GCAGTG 2 cut(s) 638, 865
BtsIMutI CAGTG 4 cut(s) 430, 638, 797, 865
BveI ACCTGC 1 cut(s) 924
Cac8I GCNNGC 1 cut(s) 872
CciI TCATGA 1 cut(s) 825
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 716
Csp6I GTAC 2 cut(s) 434, 676
CviQI GTAC 2 cut(s) 434, 676
DdeI CTNAG 4 cut(s) 444, 741, 1020, 1043
DpnI GATC 3 cut(s) 711, 942, 1038
DpnII GATC 3 cut(s) 709, 940, 1036
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 3 cut(s) 41, 576, 886
EagI CGGCCG 1 cut(s) 886
Eam1104I CTCTTC 3 cut(s) 203, 324, 800
EarI CTCTTC 3 cut(s) 203, 324, 800
EclXI CGGCCG 1 cut(s) 886
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 716
Eco52I CGGCCG 1 cut(s) 886
Eco57I CTGAAG 3 cut(s) 267, 305, 1008
EcoT22I ATGCAT 1 cut(s) 442
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FauNDI CATATG 1 cut(s) 96
Fnu4HI GCNGC 4 cut(s) 41, 469, 631, 889
FokI GGATG 4 cut(s) 946, 987, 1037, 1144
Fsp4HI GCNGC 4 cut(s) 41, 469, 631, 889
FspBI CTAG 1 cut(s) 243
GlaI GCGC 1 cut(s) 34
GluI GCNGC 4 cut(s) 41, 469, 631, 889
HaeIII GGCC 5 cut(s) 43, 408, 578, 874, 888
HapII CCGG 2 cut(s) 362, 714
HhaI GCGC 1 cut(s) 35
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 5 cut(s) 235, 358, 570, 1018, 1111
HpaII CCGG 2 cut(s) 362, 714
Hpy166II GTNNAC 6 cut(s) 92, 500, 678, 719, 788, 798
Hpy188I TCNGA 6 cut(s) 150, 175, 625, 904, 933, 988
Hpy188III TCNNGA 3 cut(s) 826, 1022, 1172
Hpy8I GTNNAC 6 cut(s) 92, 500, 678, 719, 788, 798
Hpy99I CGWCG 1 cut(s) 175
HpyAV CCTTC 3 cut(s) 18, 934, 983
HpyCH4III ACNGT 4 cut(s) 701, 792, 839, 1102
HpyCH4IV ACGT 2 cut(s) 368, 1055
HpyF10VI GCNNNNNNNGC 4 cut(s) 751, 894, 916, 953
HpyF3I CTNAG 4 cut(s) 444, 741, 1020, 1043
HpySE526I ACGT 2 cut(s) 368, 1055
HspAI GCGC 1 cut(s) 33
Kzo9I GATC 3 cut(s) 709, 940, 1036
Lsp1109I GCAGC 1 cut(s) 617
LweI GCATC 6 cut(s) 287, 449, 495, 741, 1009, 1078
MaeI CTAG 1 cut(s) 243
MaeII ACGT 2 cut(s) 368, 1055
MaeIII GTNAC 4 cut(s) 369, 967, 1027, 1078
MalI GATC 3 cut(s) 711, 942, 1038
MboI GATC 3 cut(s) 709, 940, 1036
MboII GAAGA 6 cut(s) 190, 273, 311, 340, 817, 1031
MfeI CAATTG 2 cut(s) 17, 47
MflI RGATCY 2 cut(s) 709, 940
MhlI GDGCHC 1 cut(s) 368
MlsI TGGCCA 1 cut(s) 578
MluNI TGGCCA 1 cut(s) 578
MlyI GAGTC 5 cut(s) 229, 352, 564, 1012, 1105
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 7 cut(s) 324, 327, 373, 406, 550, 801, 1101
Mox20I TGGCCA 1 cut(s) 578
Mph1103I ATGCAT 1 cut(s) 442
MroXI GAANNNNTTC 2 cut(s) 544, 784
MscI TGGCCA 1 cut(s) 578
MseI TTAA 1 cut(s) 306
MslI CAYNNNNRTG 1 cut(s) 1076
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 630
MspI CCGG 2 cut(s) 362, 714
MspR9I CCNGG 2 cut(s) 363, 714
MunI CAATTG 2 cut(s) 17, 47
MwoI GCNNNNNNNGC 4 cut(s) 751, 894, 916, 953
NciI CCSGG 2 cut(s) 363, 714
NdeI CATATG 1 cut(s) 96
NdeII GATC 3 cut(s) 709, 940, 1036
NlaIV GGNNCC 1 cut(s) 711
NmuCI GTSAC 2 cut(s) 1027, 1078
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 5 cut(s) 192, 440, 464, 818, 1079
NspV TTCGAA 1 cut(s) 778
OliI CACNNNNGTG 1 cut(s) 1076
PagI TCATGA 1 cut(s) 825
PciI ACATGT 2 cut(s) 460, 1075
PcsI WCGNNNNNNNCGW 1 cut(s) 332
PdmI GAANNNNTTC 2 cut(s) 544, 784
PkrI GCNGC 4 cut(s) 42, 470, 632, 890
PleI GAGTC 5 cut(s) 229, 352, 564, 1012, 1105
PpsI GAGTC 5 cut(s) 229, 352, 564, 1012, 1105
PscI ACATGT 2 cut(s) 460, 1075
PspN4I GGNNCC 1 cut(s) 711
PspPI GGNCC 1 cut(s) 716
PstI CTGCAG 1 cut(s) 872
PsuI RGATCY 2 cut(s) 709, 940
PvuII CAGCTG 1 cut(s) 630
RsaI GTAC 2 cut(s) 435, 677
RsaNI GTAC 2 cut(s) 434, 676
RseI CAYNNNNRTG 1 cut(s) 1076
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 4 cut(s) 41, 469, 631, 889
Sau3AI GATC 3 cut(s) 709, 940, 1036
Sau96I GGNCC 1 cut(s) 716
SchI GAGTC 5 cut(s) 229, 352, 564, 1012, 1105
ScrFI CCNGG 2 cut(s) 363, 714
SduI GDGCHC 1 cut(s) 368
SfaNI GCATC 6 cut(s) 287, 449, 495, 741, 1009, 1078
SfcI CTRYAG 1 cut(s) 868
SfuI TTCGAA 1 cut(s) 778
SinI GGWCC 1 cut(s) 716
SmiMI CAYNNNNRTG 1 cut(s) 1076
SsiI CCGC 4 cut(s) 40, 469, 733, 889
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 2 cut(s) 361, 712
TaaI ACNGT 4 cut(s) 701, 792, 839, 1102
TaiI ACGT 2 cut(s) 371, 1058
TaqI TCGA 3 cut(s) 326, 378, 778
TatI WGTACW 2 cut(s) 433, 675
TauI GCSGC 3 cut(s) 43, 471, 891
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 4 cut(s) 430, 645, 797, 872
TseFI GTSAC 2 cut(s) 1027, 1078
TseI GCWGC 1 cut(s) 630
Tsp45I GTSAC 2 cut(s) 1027, 1078
TspDTI ATGAA 5 cut(s) 113, 337, 818, 950, 1148
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 4 cut(s) 430, 645, 797, 872
VpaK11BI GGWCC 1 cut(s) 716
XapI RAATTY 5 cut(s) 300, 394, 419, 542, 773
XceI RCATGY 5 cut(s) 192, 440, 464, 818, 1079
XmnI GAANNNNTTC 2 cut(s) 544, 784
XspI CTAG 1 cut(s) 243
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.