Rh6CG527700

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0004591
Physical Location & Seq
Reverse (-)
68179372 .. 68183524
4153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG527700.1

Sequence Viewer

Length: 1200 bp
ATGTTGCCTTCAACCCAATTGAAAAAAGGTGGCGCATGGAGGCCACAATTGTTCAAAGACATTATGGTCATGTCCAAGTCAAGGTCTCGTTCACAAACGAAAAGACCAAGATGGATTATTGCATTGGTTTGTATCCTCTGCTTTTTTCTGATTACTGCTTATTTCTATCCGTCGGAAAGCTCTACGACATGCTATCTCTTCTCATCTCCTAATTGTGATGGGATATTTGGAAAGACTCCACCTAGTCCTGCAAGGGAACTAACTGACGACGAGACTGCATCTTCAGTTGTAGTTAGGGAAATTCTCAATACGCCTCCTCTTCAGTCGAAGAACCCGAAAATCGCTTTCATGTTTTTGACTCCGGGCACGTTACCTTTCGAGGAGTTATGGGAAAATTTCTTTCAAGGCCATGAGGACAAATTCAGTGTTTATGTACATGCATCTAAGCAGAAACCAATACATGTGAGCCGCTATTTTGCTGATAGAGACATACATAGTGAACAGGTAGTTTGGGGAAAATTTTCAATGGTTGAGGCAGAAAAGAGACTCTTGGCCAATGCACTTTTAGACCCTGATAACCAACACTTTGTTCTGCTGTCTGACAGCTGCATACCACTGCATAGCTTTGATTATGTATATAATTATCTCATGTACACGAATGTCAGCTTTATAGACTGTTTTGTGGATCCCGGTCCACATGGAACTGGACGGTATGCTGAGCAAATGATGCCCGAAGTTGAAAAGCACGAATTTCGAAAGGGTTCACAGTGGTTCACTATGAAGAGGCAACATGCTATTGTCATGATGGCAGACAGTCTATACTATACCAAGTTCAAGCTTCACTGCAGGCCGAACATGGACGGGCGCAACTGCTATTCCGACGAGCATTACCTGCCAACCTTCTTTCGGATGAAAGATCCTGCTGGTCTTGCAAATTGGTCGGTAACATTTGTTGATTGGTCTGAAGGGAAATGGCATCCAAGAGCATTTAGGACTCAGGATGTTACTTATGATCTTCTTAGGAACATCACGTCCATTGCTGATAGCACACATGTGACAAGTGATGCAACGAAAACAGTATTGAGGACTCCTTGCCTGTGGAATGGGATGAAGCGACCCTGTTTCTTATTTGCCAGAAAGTTCTATCCTGAAACTCTGGGGAAACTGATACACCTTTTCTCCAATTATACTAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

399

Amino Acids

46.36

Weight (kDa)

8.77

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 114 - 343 1.2e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 900
AciI CCGC 1 cut(s) 469
AclWI GGATC 3 cut(s) 680, 693, 911
AcoI YGGCCR 1 cut(s) 552
AcsI RAATTY 5 cut(s) 300, 394, 419, 518, 749
AcuI CTGAAG 3 cut(s) 267, 305, 984
AfaI GTAC 2 cut(s) 435, 653
AfiI CCNNNNNNNGG 2 cut(s) 81, 906
AflIII ACRYGT 2 cut(s) 460, 1051
AgsI TTSAA 7 cut(s) 12, 22, 55, 404, 525, 740, 835
AjiI CACGTC 1 cut(s) 1032
AjuI GAANNNNNNNTTGG 2 cut(s) 573, 605
AleI CACNNNNGTG 1 cut(s) 1052
AloI GAACNNNNNNTCC 2 cut(s) 1016, 1048
AluBI AGCT 5 cut(s) 180, 606, 624, 666, 838
AluI AGCT 5 cut(s) 180, 606, 624, 666, 838
Alw26I GTCTC 4 cut(s) 90, 266, 480, 538
AlwI GGATC 3 cut(s) 680, 693, 911
AoxI GGCC 4 cut(s) 41, 406, 552, 848
ApeKI GCWGC 1 cut(s) 606
ApoI RAATTY 5 cut(s) 300, 394, 419, 518, 749
Asp700I GAANNNNTTC 2 cut(s) 520, 760
AspLEI GCGC 2 cut(s) 35, 867
AspS9I GGNCC 1 cut(s) 692
AsuC2I CCSGG 2 cut(s) 363, 690
AsuII TTCGAA 1 cut(s) 754
AvaII GGWCC 1 cut(s) 692
BaeGI GKGCMC 1 cut(s) 368
BalI TGGCCA 1 cut(s) 554
BamHI GGATCC 1 cut(s) 685
BbvI GCAGC 1 cut(s) 593
BccI CCATC 3 cut(s) 105, 212, 799
BcgI CGANNNNNNTGC 6 cut(s) 257, 291, 734, 768, 921, 955
BciVI GTATCC 1 cut(s) 143
BcnI CCSGG 2 cut(s) 363, 690
BcoDI GTCTC 4 cut(s) 90, 266, 480, 538
BfaI CTAG 1 cut(s) 243
BfmI CTRYAG 1 cut(s) 844
BfuAI ACCTGC 1 cut(s) 900
BfuI GTATCC 1 cut(s) 143
BisI GCNGC 2 cut(s) 469, 607
BlpI GCTNAGC 1 cut(s) 717
BlsI GCNGC 2 cut(s) 470, 608
Bme1390I CCNGG 2 cut(s) 363, 690
Bme18I GGWCC 1 cut(s) 692
BmgBI CACGTC 1 cut(s) 1032
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 1 cut(s) 687
BmrFI CCNGG 2 cut(s) 363, 690
BmsI GCATC 5 cut(s) 287, 449, 717, 985, 1054
Bpu1102I GCTNAGC 1 cut(s) 717
Bpu14I TTCGAA 1 cut(s) 754
BpuMI CCSGG 2 cut(s) 363, 690
BsaI GGTCTC 1 cut(s) 90
BsaXI ACNNNNNCTCC 2 cut(s) 1163, 1193
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 906
Bse1I ACTGG 1 cut(s) 709
Bse3DI GCAATG 1 cut(s) 1035
BseGI GGATG 4 cut(s) 915, 976, 1006, 1113
BseLI CCNNNNNNNGG 2 cut(s) 81, 906
BseMI GCAATG 1 cut(s) 1035
BseMII CTCAG 2 cut(s) 708, 1010
BseNI ACTGG 1 cut(s) 709
BseRI GAGGAG 2 cut(s) 306, 395
BseSI GKGCMC 1 cut(s) 368
BseXI GCAGC 1 cut(s) 593
BshFI GGCC 4 cut(s) 43, 408, 554, 850
BsiSI CCGG 2 cut(s) 362, 690
BslI CCNNNNNNNGG 2 cut(s) 81, 906
BsmAI GTCTC 4 cut(s) 90, 266, 480, 538
BsnI GGCC 4 cut(s) 43, 408, 554, 850
Bso31I GGTCTC 1 cut(s) 90
Bsp119I TTCGAA 1 cut(s) 754
Bsp1286I GDGCHC 1 cut(s) 368
Bsp1407I TGTACA 2 cut(s) 433, 651
Bsp143I GATC 3 cut(s) 685, 916, 1012
Bsp1720I GCTNAGC 1 cut(s) 717
BspACI CCGC 1 cut(s) 469
BspANI GGCC 4 cut(s) 43, 408, 554, 850
BspCNI CTCAG 2 cut(s) 709, 1009
BspHI TCATGA 1 cut(s) 801
BspLI GGNNCC 1 cut(s) 687
BspMAI CTGCAG 1 cut(s) 848
BspMI ACCTGC 1 cut(s) 900
BspPI GGATC 3 cut(s) 680, 693, 911
BspT104I TTCGAA 1 cut(s) 754
BspTNI GGTCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 1035
BsrGI TGTACA 2 cut(s) 433, 651
BsrI ACTGG 1 cut(s) 709
BssMI GATC 3 cut(s) 685, 916, 1012
Bst4CI ACNGT 5 cut(s) 677, 711, 768, 815, 1078
Bst6I CTCTTC 3 cut(s) 203, 324, 776
BstAPI GCANNNNNTGC 2 cut(s) 727, 892
BstAUI TGTACA 2 cut(s) 433, 651
BstBI TTCGAA 1 cut(s) 754
BstC8I GCNNGC 1 cut(s) 848
BstDEI CTNAG 4 cut(s) 444, 717, 996, 1019
BstF5I GGATG 4 cut(s) 915, 976, 1006, 1113
BstHHI GCGC 2 cut(s) 35, 867
BstKTI GATC 3 cut(s) 688, 919, 1015
BstMAI GTCTC 4 cut(s) 90, 266, 480, 538
BstMBI GATC 3 cut(s) 685, 916, 1012
BstMWI GCNNNNNNNGC 3 cut(s) 727, 892, 929
BstNSI RCATGY 5 cut(s) 192, 440, 464, 794, 1055
BstSCI CCNGG 2 cut(s) 361, 688
BstSFI CTRYAG 1 cut(s) 844
BstSLI GKGCMC 1 cut(s) 368
BstV1I GCAGC 1 cut(s) 593
BstX2I RGATCY 2 cut(s) 685, 916
BstYI RGATCY 2 cut(s) 685, 916
BsuI GTATCC 1 cut(s) 143
BsuRI GGCC 4 cut(s) 43, 408, 554, 850
BtrI CACGTC 1 cut(s) 1032
BtsCI GGATG 4 cut(s) 915, 976, 1006, 1113
BtsI GCAGTG 2 cut(s) 614, 841
BtsIMutI CAGTG 4 cut(s) 430, 614, 773, 841
BveI ACCTGC 1 cut(s) 900
Cac8I GCNNGC 1 cut(s) 848
CciI TCATGA 1 cut(s) 801
CfoI GCGC 2 cut(s) 35, 867
Cfr13I GGNCC 1 cut(s) 692
Csp6I GTAC 2 cut(s) 434, 652
CviQI GTAC 2 cut(s) 434, 652
DdeI CTNAG 4 cut(s) 444, 717, 996, 1019
DpnI GATC 3 cut(s) 687, 918, 1014
DpnII GATC 3 cut(s) 685, 916, 1012
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 1 cut(s) 552
Eam1104I CTCTTC 3 cut(s) 203, 324, 776
EarI CTCTTC 3 cut(s) 203, 324, 776
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 692
Eco57I CTGAAG 3 cut(s) 267, 305, 984
EcoT22I ATGCAT 1 cut(s) 442
FalI AAGNNNNNCTT 2 cut(s) 533, 565
Fnu4HI GCNGC 2 cut(s) 469, 607
FokI GGATG 4 cut(s) 922, 963, 1013, 1120
Fsp4HI GCNGC 2 cut(s) 469, 607
FspBI CTAG 1 cut(s) 243
GlaI GCGC 2 cut(s) 34, 866
GluI GCNGC 2 cut(s) 469, 607
HaeIII GGCC 4 cut(s) 43, 408, 554, 850
HapII CCGG 2 cut(s) 362, 690
HhaI GCGC 2 cut(s) 35, 867
Hin6I GCGC 2 cut(s) 33, 865
HinP1I GCGC 2 cut(s) 33, 865
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 5 cut(s) 235, 358, 546, 994, 1087
HpaII CCGG 2 cut(s) 362, 690
Hpy166II GTNNAC 6 cut(s) 92, 500, 654, 695, 764, 774
Hpy188I TCNGA 6 cut(s) 150, 175, 601, 880, 909, 964
Hpy188III TCNNGA 3 cut(s) 802, 998, 1148
Hpy8I GTNNAC 6 cut(s) 92, 500, 654, 695, 764, 774
Hpy99I CGWCG 3 cut(s) 175, 272, 884
HpyAV CCTTC 3 cut(s) 18, 910, 959
HpyCH4III ACNGT 5 cut(s) 677, 711, 768, 815, 1078
HpyCH4IV ACGT 2 cut(s) 368, 1031
HpyF10VI GCNNNNNNNGC 3 cut(s) 727, 892, 929
HpyF3I CTNAG 4 cut(s) 444, 717, 996, 1019
HpySE526I ACGT 2 cut(s) 368, 1031
HspAI GCGC 2 cut(s) 33, 865
Kzo9I GATC 3 cut(s) 685, 916, 1012
Lsp1109I GCAGC 1 cut(s) 593
LweI GCATC 5 cut(s) 287, 449, 717, 985, 1054
MaeI CTAG 1 cut(s) 243
MaeII ACGT 2 cut(s) 368, 1031
MaeIII GTNAC 4 cut(s) 369, 943, 1003, 1054
MalI GATC 3 cut(s) 687, 918, 1014
MboI GATC 3 cut(s) 685, 916, 1012
MboII GAAGA 6 cut(s) 190, 273, 311, 340, 793, 1007
MfeI CAATTG 2 cut(s) 17, 47
MflI RGATCY 2 cut(s) 685, 916
MhlI GDGCHC 1 cut(s) 368
MlsI TGGCCA 1 cut(s) 554
MluNI TGGCCA 1 cut(s) 554
MlyI GAGTC 5 cut(s) 229, 352, 540, 988, 1081
MmeI TCCRAC 2 cut(s) 153, 903
MnlI CCTC 9 cut(s) 33, 146, 324, 327, 373, 406, 526, 777, 1077
Mox20I TGGCCA 1 cut(s) 554
Mph1103I ATGCAT 1 cut(s) 442
MroXI GAANNNNTTC 2 cut(s) 520, 760
MscI TGGCCA 1 cut(s) 554
MslI CAYNNNNRTG 1 cut(s) 1052
Msp20I TGGCCA 1 cut(s) 554
MspA1I CMGCKG 1 cut(s) 606
MspI CCGG 2 cut(s) 362, 690
MspR9I CCNGG 2 cut(s) 363, 690
MunI CAATTG 2 cut(s) 17, 47
MwoI GCNNNNNNNGC 3 cut(s) 727, 892, 929
NciI CCSGG 2 cut(s) 363, 690
NdeII GATC 3 cut(s) 685, 916, 1012
NlaIV GGNNCC 1 cut(s) 687
NmuCI GTSAC 1 cut(s) 1054
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 5 cut(s) 192, 440, 464, 794, 1055
NspV TTCGAA 1 cut(s) 754
OliI CACNNNNGTG 1 cut(s) 1052
PagI TCATGA 1 cut(s) 801
PciI ACATGT 2 cut(s) 460, 1051
PcsI WCGNNNNNNNCGW 1 cut(s) 332
PdmI GAANNNNTTC 2 cut(s) 520, 760
PkrI GCNGC 2 cut(s) 470, 608
PleI GAGTC 5 cut(s) 229, 352, 540, 988, 1081
PpsI GAGTC 5 cut(s) 229, 352, 540, 988, 1081
PscI ACATGT 2 cut(s) 460, 1051
PspN4I GGNNCC 1 cut(s) 687
PspPI GGNCC 1 cut(s) 692
PstI CTGCAG 1 cut(s) 848
PsuI RGATCY 2 cut(s) 685, 916
PvuII CAGCTG 1 cut(s) 606
RsaI GTAC 2 cut(s) 435, 653
RsaNI GTAC 2 cut(s) 434, 652
RseI CAYNNNNRTG 1 cut(s) 1052
SatI GCNGC 2 cut(s) 469, 607
Sau3AI GATC 3 cut(s) 685, 916, 1012
Sau96I GGNCC 1 cut(s) 692
SchI GAGTC 5 cut(s) 229, 352, 540, 988, 1081
ScrFI CCNGG 2 cut(s) 363, 690
SduI GDGCHC 1 cut(s) 368
SfaNI GCATC 5 cut(s) 287, 449, 717, 985, 1054
SfcI CTRYAG 1 cut(s) 844
SfuI TTCGAA 1 cut(s) 754
SinI GGWCC 1 cut(s) 692
SmiMI CAYNNNNRTG 1 cut(s) 1052
SsiI CCGC 1 cut(s) 469
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 2 cut(s) 361, 688
TaaI ACNGT 5 cut(s) 677, 711, 768, 815, 1078
TaiI ACGT 2 cut(s) 371, 1034
TaqI TCGA 3 cut(s) 326, 378, 754
TatI WGTACW 2 cut(s) 433, 651
TauI GCSGC 1 cut(s) 471
TscAI CASTG 4 cut(s) 430, 621, 773, 848
TseFI GTSAC 1 cut(s) 1054
TseI GCWGC 1 cut(s) 606
Tsp45I GTSAC 1 cut(s) 1054
TspDTI ATGAA 4 cut(s) 337, 794, 926, 1124
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 4 cut(s) 430, 621, 773, 848
VpaK11BI GGWCC 1 cut(s) 692
XapI RAATTY 5 cut(s) 300, 394, 419, 518, 749
XceI RCATGY 5 cut(s) 192, 440, 464, 794, 1055
XmnI GAANNNNTTC 2 cut(s) 520, 760
XspI CTAG 1 cut(s) 243
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.