pycom08g11290

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004591
Physical Location & Seq
Forward (+)
9575624 .. 9577314
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g11290.2

Sequence Viewer

Length: 561 bp
ATGAGAGGTTCTGTGTTGCCTTCGATCCAGATGAAAAAGTCATTTGCATGGCGGCCGCAACTGATGAGAGATACGGTGATGTCTAAGTCAAGGTCGCGTCCAGCGTTGAGAAGACCAACATGGATCCTTGCATTGGTTTCGTTCATCTGTGTTTTTCTGATACTTGCCTACTTCTACTCTCCGAAAAACTCTGCCTCCTGCAATCTGTTTTCTTCTAGTGGCTGTCTCTTTGGAAGGACTCCCCCTAGCCCTGCGAGGGAGCTGACCGATGATGAGACTGCGTCGGAGGTTGTAGTTAGGGAGATTCTCAAGACACCTCCTCTTCAATCCAAGAACCCGAAAATTGCTTTCATGTTTTTGACTCCCGGAACTTTGCCTTTCGAGGAGTTGTGGGAAAATTTCTTCCAAGGCCACGAAGACAAATTTACCGTTTATGTACATGCATCCAAGGAAAAACCAGTACATGCGAGCCGGTATTTTGCTGACAGAGACATACATAGTGAACAGGTATGCAGAGCCTCTCGGATAATGCTTGCAGTACAGTATTATTTTTTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

187

Amino Acids

21.4

Weight (kDa)

9.51

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 97
AciI CCGC 2 cut(s) 52, 56
AclWI GGATC 3 cut(s) 19, 118, 131
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 2 cut(s) 397, 422
AfaI GTAC 3 cut(s) 438, 462, 540
AfiI CCNNNNNNNGG 2 cut(s) 133, 256
AgsI TTSAA 1 cut(s) 326
AluBI AGCT 1 cut(s) 262
AluI AGCT 1 cut(s) 262
Alw26I GTCTC 3 cut(s) 230, 269, 483
AlwI GGATC 3 cut(s) 19, 118, 131
AoxI GGCC 2 cut(s) 53, 409
ApoI RAATTY 2 cut(s) 397, 422
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 1 cut(s) 88
BamHI GGATCC 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 118, 423
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 3 cut(s) 230, 269, 483
BfaI CTAG 2 cut(s) 216, 246
BisI GCNGC 2 cut(s) 53, 56
BlsI GCNGC 2 cut(s) 54, 57
Bme1390I CCNGG 1 cut(s) 366
BmiI GGNNCC 1 cut(s) 125
BmrFI CCNGG 1 cut(s) 366
BmsI GCATC 1 cut(s) 452
BpiI GAAGAC 2 cut(s) 118, 423
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 406, 447
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 256
Bse118I RCCGGY 1 cut(s) 471
Bse1I ACTGG 1 cut(s) 458
BseDI CCNNGG 2 cut(s) 406, 447
BseGI GGATG 1 cut(s) 443
BseLI CCNNNNNNNGG 2 cut(s) 133, 256
BseNI ACTGG 1 cut(s) 458
BseRI GAGGAG 2 cut(s) 309, 398
BseX3I CGGCCG 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 97
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 2 cut(s) 55, 411
BsiEI CGRYCG 1 cut(s) 56
BsiSI CCGG 2 cut(s) 366, 472
BslI CCNNNNNNNGG 2 cut(s) 133, 256
BsmAI GTCTC 3 cut(s) 230, 269, 483
BsnI GGCC 2 cut(s) 55, 411
Bsp1407I TGTACA 1 cut(s) 436
Bsp143I GATC 2 cut(s) 24, 123
BspACI CCGC 2 cut(s) 52, 56
BspANI GGCC 2 cut(s) 55, 411
BspFNI CGCG 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 125
BspPI GGATC 3 cut(s) 19, 118, 131
BsrFI RCCGGY 1 cut(s) 471
BsrGI TGTACA 1 cut(s) 436
BsrI ACTGG 1 cut(s) 458
BssAI RCCGGY 1 cut(s) 471
BssECI CCNNGG 2 cut(s) 406, 447
BssMI GATC 2 cut(s) 24, 123
BssT1I CCWWGG 2 cut(s) 406, 447
Bst4CI ACNGT 3 cut(s) 76, 430, 543
Bst6I CTCTTC 1 cut(s) 327
BstAUI TGTACA 1 cut(s) 436
BstC8I GCNNGC 2 cut(s) 469, 534
BstDEI CTNAG 1 cut(s) 84
BstF5I GGATG 1 cut(s) 443
BstFNI CGCG 1 cut(s) 97
BstKTI GATC 2 cut(s) 27, 126
BstMAI GTCTC 3 cut(s) 230, 269, 483
BstMBI GATC 2 cut(s) 24, 123
BstMCI CGRYCG 1 cut(s) 56
BstNSI RCATGY 2 cut(s) 443, 467
BstSCI CCNGG 1 cut(s) 364
BstUI CGCG 1 cut(s) 97
BstV2I GAAGAC 2 cut(s) 118, 423
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BstZI CGGCCG 1 cut(s) 53
BsuRI GGCC 2 cut(s) 55, 411
BtsCI GGATG 1 cut(s) 443
Cac8I GCNNGC 2 cut(s) 469, 534
CciNI GCGGCCGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 471
CseI GACGC 2 cut(s) 86, 270
Csp6I GTAC 3 cut(s) 437, 461, 539
CviAII CATG 5 cut(s) 48, 120, 352, 440, 464
CviJI RGCY 7 cut(s) 55, 222, 249, 262, 411, 471, 518
CviKI_1 RGCY 7 cut(s) 55, 222, 249, 262, 411, 471, 518
CviQI GTAC 3 cut(s) 437, 461, 539
DdeI CTNAG 1 cut(s) 84
DpnI GATC 2 cut(s) 26, 125
DpnII GATC 2 cut(s) 24, 123
EaeI YGGCCR 1 cut(s) 53
EagI CGGCCG 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
EclXI CGGCCG 1 cut(s) 53
Eco130I CCWWGG 2 cut(s) 406, 447
Eco52I CGGCCG 1 cut(s) 53
EcoT14I CCWWGG 2 cut(s) 406, 447
EcoT22I ATGCAT 1 cut(s) 445
ErhI CCWWGG 2 cut(s) 406, 447
FaeI CATG 5 cut(s) 51, 123, 355, 443, 467
FaiI YATR 9 cut(s) 49, 121, 353, 435, 441, 465, 494, 498, 511
FatI CATG 5 cut(s) 47, 119, 351, 439, 463
Fnu4HI GCNGC 2 cut(s) 53, 56
FokI GGATG 1 cut(s) 430
Fsp4HI GCNGC 2 cut(s) 53, 56
FspBI CTAG 2 cut(s) 216, 246
GluI GCNGC 2 cut(s) 53, 56
HaeIII GGCC 2 cut(s) 55, 411
HapII CCGG 2 cut(s) 366, 472
HgaI GACGC 2 cut(s) 86, 270
Hin1II CATG 5 cut(s) 51, 123, 355, 443, 467
HinfI GANTC 3 cut(s) 238, 304, 361
HpaII CCGG 2 cut(s) 366, 472
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 1 cut(s) 503
Hpy188I TCNGA 4 cut(s) 159, 183, 286, 525
Hpy188III TCNNGA 3 cut(s) 28, 310, 558
Hpy8I GTNNAC 1 cut(s) 503
Hpy99I CGWCG 1 cut(s) 286
HpyAV CCTTC 2 cut(s) 30, 228
HpyCH4III ACNGT 3 cut(s) 76, 430, 543
HpyCH4V TGCA 6 cut(s) 47, 131, 201, 443, 513, 536
HpyF3I CTNAG 1 cut(s) 84
Hsp92II CATG 5 cut(s) 51, 123, 355, 443, 467
Kzo9I GATC 2 cut(s) 24, 123
LmnI GCTCC 1 cut(s) 259
LpnPI CCDG 8 cut(s) 41, 114, 211, 264, 379, 471, 485, 491
LweI GCATC 1 cut(s) 452
MaeI CTAG 2 cut(s) 216, 246
MalI GATC 2 cut(s) 26, 125
MboI GATC 2 cut(s) 24, 123
MboII GAAGA 5 cut(s) 123, 204, 314, 394, 428
MflI RGATCY 1 cut(s) 123
MluCI AATT 3 cut(s) 342, 397, 422
MlyI GAGTC 2 cut(s) 232, 355
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 7 cut(s) 205, 249, 280, 327, 330, 376, 529
Mph1103I ATGCAT 1 cut(s) 445
MslI CAYNNNNRTG 1 cut(s) 46
MspI CCGG 2 cut(s) 366, 472
MspR9I CCNGG 1 cut(s) 366
MvnI CGCG 1 cut(s) 97
NciI CCSGG 1 cut(s) 366
NdeII GATC 2 cut(s) 24, 123
NlaIII CATG 5 cut(s) 51, 123, 355, 443, 467
NlaIV GGNNCC 1 cut(s) 125
NotI GCGGCCGC 1 cut(s) 53
NsiI ATGCAT 1 cut(s) 445
NspI RCATGY 2 cut(s) 443, 467
PcsI WCGNNNNNNNCGW 1 cut(s) 101
PfeI GAWTC 1 cut(s) 304
PflFI GACNNNGTC 1 cut(s) 280
PfoI TCCNGGA 1 cut(s) 364
PkrI GCNGC 2 cut(s) 54, 57
PleI GAGTC 2 cut(s) 232, 355
PpsI GAGTC 2 cut(s) 232, 355
PspN4I GGNNCC 1 cut(s) 125
PsuI RGATCY 1 cut(s) 123
PsyI GACNNNGTC 1 cut(s) 280
RsaI GTAC 3 cut(s) 438, 462, 540
RsaNI GTAC 3 cut(s) 437, 461, 539
RseI CAYNNNNRTG 1 cut(s) 46
SatI GCNGC 2 cut(s) 53, 56
Sau3AI GATC 2 cut(s) 24, 123
SchI GAGTC 2 cut(s) 232, 355
ScrFI CCNGG 1 cut(s) 366
SetI ASST 6 cut(s) 10, 95, 264, 291, 319, 510
SfaNI GCATC 1 cut(s) 452
SmiMI CAYNNNNRTG 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 3 cut(s) 342, 397, 422
SsiI CCGC 2 cut(s) 52, 56
SspMI CTAG 2 cut(s) 216, 246
StyD4I CCNGG 1 cut(s) 364
StyI CCWWGG 2 cut(s) 406, 447
TaaI ACNGT 3 cut(s) 76, 430, 543
TaqI TCGA 2 cut(s) 23, 381
TaqII GACCGA 1 cut(s) 281
TasI AATT 3 cut(s) 342, 397, 422
TatI WGTACW 3 cut(s) 436, 460, 538
TauI GCSGC 2 cut(s) 55, 58
TfiI GAWTC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 47, 133, 340
Tth111I GACNNNGTC 1 cut(s) 280
XapI RAATTY 2 cut(s) 397, 422
XceI RCATGY 2 cut(s) 443, 467
XspI CTAG 2 cut(s) 216, 246
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.