MD08G1134100.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004591
Physical Location & Seq
Forward (+)
12777231 .. 12781307
4077 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1134100.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGAAAAAGTCGTTCGCATGGCGGCCGCAACTGATGAGAGATACGGTGATGTCTAAGTCAAGGTCGCGTCCAGCGTTGAGAAGACCAACATGGATCATTGCATTGGTTTCGTTCATCTGTGTTTTTCTGATACTTGCCTACTTCTACTCTCCGAAAAACTCTGCCTCCTGCAATCTGTTTTCTTCTAGTGGCTGTCTGTTTGGAAGGACTCCCCCTAGCCCTGCGAGGGAGCTGACCGATGATGAGACTGCGTCGGAGGTTGTAGTTAGGGAGATTCTCAAGACGCCTCCTCTTCAATCCAAGAACCCGAAAATTGCTTTCATGTTTTTGACTCCCGGAACCTTGCCTTTCGAGGAGTTGTGGGAAAATTTCTTCCAAGGCCATGAAGACAAATTTACCGTTTATGTACATGCATCCAAGGAAAAACCAGTACATGCGAGCCGGTATTTTGCTGACAGAGACATACATAGTGAACAGGTCGTTTGGGGAAAATTTTCGATGGTTGAGGCAGAGAAGAGACTTTTGGCAAATGCACTTTTAGACCCCGACAACCAACATTTTGTCTTGCTGTCTGATAGTTGCATACCACTGCATAACTTCGATTATGTATATAACTATCTCATGTACACAAACGTCAGCTTTATCGATTGTTTTGTTGATCCTGGTCCGCATGGAACTGGCCGGTATTCAAAACATATGTTGCCTGAAGTTGACAGGAGCAATTTCCGGAAGGGTTCACAGTGGTTCTCTATTAAGCGGCAACACGCAATTGTCATGATGGCAGACTGTCTATACTATACAAAGTTCAAGCTCTTCTGCAGGCCAAACTTGGATGGACGCAACTGCTATTCGGATGAGCATTACTTGCCAACCTTCTTCAATCTGAAAGATCCTGGTGGACTCGCAAATTGGTCTGTAACATATGTCGATTGGTCTGAAGGGAAGTGGCATCCAAGAGCGTTCAGAACTCAGGATGTTACTTATGACCTTCTTAGGAACATCACGTCCATTGCTGAGAGCACGCATATTACAAGCGAAGACAAGAAGCGAATTCTGAACACTCCGTGCTTGTGGAACGGGATGAAGCGACCGTGTTATCTATTTGCCCGAAAGTTCTATCCAGAAACACTGGGGAAACTGGTTCAGCTTTTCTCCAATTACACAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

391

Amino Acids

45.41

Weight (kDa)

9.01

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 105 - 334 1.8e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 67
AccIII TCCGGA 1 cut(s) 726
AciI CCGC 4 cut(s) 22, 26, 668, 757
AclWI GGATC 3 cut(s) 101, 653, 884
AcoI YGGCCR 2 cut(s) 23, 679
AcsI RAATTY 4 cut(s) 367, 392, 491, 1050
AcuI CTGAAG 2 cut(s) 726, 957
AcyI GRCGYC 1 cut(s) 284
AdeI CACNNNGTG 1 cut(s) 1065
AfaI GTAC 3 cut(s) 408, 432, 626
AfiI CCNNNNNNNGG 1 cut(s) 226
AgsI TTSAA 4 cut(s) 296, 690, 808, 880
AjiI CACGTC 1 cut(s) 1005
AjnI CCWGG 2 cut(s) 661, 892
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AloI GAACNNNNNNTCC 2 cut(s) 989, 1021
AluBI AGCT 4 cut(s) 232, 639, 811, 1147
AluI AGCT 4 cut(s) 232, 639, 811, 1147
Alw21I GWGCWC 1 cut(s) 1022
Alw26I GTCTC 3 cut(s) 239, 453, 511
AlwI GGATC 3 cut(s) 101, 653, 884
Aor13HI TCCGGA 1 cut(s) 726
AoxI GGCC 4 cut(s) 23, 379, 679, 821
ApoI RAATTY 4 cut(s) 367, 392, 491, 1050
Asp700I GAANNNNTTC 1 cut(s) 493
AspS9I GGNCC 1 cut(s) 665
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 1 cut(s) 58
AvaII GGWCC 1 cut(s) 665
BbsI GAAGAC 3 cut(s) 88, 393, 1044
Bbv12I GWGCWC 1 cut(s) 1022
BccI CCATC 3 cut(s) 493, 772, 827
BcgI CGANNNNNNTGC 2 cut(s) 90, 124
BciT130I CCWGG 2 cut(s) 663, 894
BcnI CCSGG 1 cut(s) 336
BcoDI GTCTC 3 cut(s) 239, 453, 511
BfaI CTAG 2 cut(s) 186, 216
BfmI CTRYAG 1 cut(s) 817
BisI GCNGC 3 cut(s) 23, 26, 758
BlsI GCNGC 3 cut(s) 24, 27, 759
Bme1390I CCNGG 3 cut(s) 336, 663, 894
Bme18I GGWCC 1 cut(s) 665
BmgBI CACGTC 1 cut(s) 1005
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 3 cut(s) 336, 663, 894
BmrI ACTGGG 1 cut(s) 1139
BmsI GCATC 2 cut(s) 422, 958
BmuI ACTGGG 1 cut(s) 1139
BpiI GAAGAC 3 cut(s) 88, 393, 1044
BpuEI CTTGAG 1 cut(s) 263
BpuMI CCSGG 1 cut(s) 336
Bsa29I ATCGAT 1 cut(s) 645
BsaHI GRCGYC 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 376, 417
BsaWI WCCGGW 1 cut(s) 726
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse118I RCCGGY 2 cut(s) 441, 681
Bse1I ACTGG 4 cut(s) 428, 682, 1134, 1143
Bse3DI GCAATG 2 cut(s) 96, 1008
BseAI TCCGGA 1 cut(s) 726
BseBI CCWGG 2 cut(s) 663, 894
BseCI ATCGAT 1 cut(s) 645
BseDI CCNNGG 2 cut(s) 376, 417
BseGI GGATG 6 cut(s) 413, 838, 859, 949, 979, 1086
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMI GCAATG 2 cut(s) 96, 1008
BseMII CTCAG 2 cut(s) 983, 1005
BseNI ACTGG 4 cut(s) 428, 682, 1134, 1143
BseRI GAGGAG 2 cut(s) 279, 368
BseX3I CGGCCG 1 cut(s) 23
Bsh1236I CGCG 1 cut(s) 67
Bsh1285I CGRYCG 2 cut(s) 26, 1091
BshFI GGCC 4 cut(s) 25, 381, 681, 823
BshVI ATCGAT 1 cut(s) 645
BsiEI CGRYCG 2 cut(s) 26, 1091
BsiHKAI GWGCWC 1 cut(s) 1022
BsiSI CCGG 4 cut(s) 336, 442, 682, 727
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 3 cut(s) 239, 453, 511
BsnI GGCC 4 cut(s) 25, 381, 681, 823
Bsp1286I GDGCHC 1 cut(s) 1022
Bsp13I TCCGGA 1 cut(s) 726
Bsp1407I TGTACA 2 cut(s) 406, 624
Bsp143I GATC 3 cut(s) 93, 658, 889
BspACI CCGC 4 cut(s) 22, 26, 668, 757
BspANI GGCC 4 cut(s) 25, 381, 681, 823
BspCNI CTCAG 2 cut(s) 982, 1006
BspDI ATCGAT 1 cut(s) 645
BspEI TCCGGA 1 cut(s) 726
BspFNI CGCG 1 cut(s) 67
BspHI TCATGA 1 cut(s) 774
BspLI GGNNCC 1 cut(s) 340
BspMAI CTGCAG 1 cut(s) 821
BspPI GGATC 3 cut(s) 101, 653, 884
BspQI GCTCTTC 1 cut(s) 818
BsrDI GCAATG 2 cut(s) 96, 1008
BsrFI RCCGGY 2 cut(s) 441, 681
BsrGI TGTACA 2 cut(s) 406, 624
BsrI ACTGG 4 cut(s) 428, 682, 1134, 1143
BssAI RCCGGY 2 cut(s) 441, 681
BssECI CCNNGG 2 cut(s) 376, 417
BssMI GATC 3 cut(s) 93, 658, 889
BssNI GRCGYC 1 cut(s) 284
BssT1I CCWWGG 2 cut(s) 376, 417
Bst2UI CCWGG 2 cut(s) 663, 894
Bst4CI ACNGT 5 cut(s) 46, 400, 741, 788, 1092
Bst6I CTCTTC 3 cut(s) 297, 509, 818
BstACI GRCGYC 1 cut(s) 284
BstAPI GCANNNNNTGC 1 cut(s) 865
BstAUI TGTACA 2 cut(s) 406, 624
BstC8I GCNNGC 3 cut(s) 439, 821, 1022
BstDEI CTNAG 4 cut(s) 54, 969, 992, 1014
BstF5I GGATG 6 cut(s) 413, 838, 859, 949, 979, 1086
BstFNI CGCG 1 cut(s) 67
BstKTI GATC 3 cut(s) 96, 661, 892
BstMAI GTCTC 3 cut(s) 239, 453, 511
BstMBI GATC 3 cut(s) 93, 658, 889
BstMCI CGRYCG 2 cut(s) 26, 1091
BstMWI GCNNNNNNNGC 1 cut(s) 865
BstNI CCWGG 2 cut(s) 663, 894
BstNSI RCATGY 2 cut(s) 413, 437
BstSCI CCNGG 3 cut(s) 334, 661, 892
BstSFI CTRYAG 1 cut(s) 817
BstUI CGCG 1 cut(s) 67
BstV2I GAAGAC 3 cut(s) 88, 393, 1044
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
BstZI CGGCCG 1 cut(s) 23
Bsu15I ATCGAT 1 cut(s) 645
BsuRI GGCC 4 cut(s) 25, 381, 681, 823
BsuTUI ATCGAT 1 cut(s) 645
BtrI CACGTC 1 cut(s) 1005
BtsCI GGATG 6 cut(s) 413, 838, 859, 949, 979, 1086
BtsI GCAGTG 1 cut(s) 587
BtsIMutI CAGTG 3 cut(s) 587, 746, 1127
Cac8I GCNNGC 3 cut(s) 439, 821, 1022
CciI TCATGA 1 cut(s) 774
CciNI GCGGCCGC 1 cut(s) 23
Cfr10I RCCGGY 2 cut(s) 441, 681
Cfr13I GGNCC 1 cut(s) 665
ClaI ATCGAT 1 cut(s) 645
CseI GACGC 4 cut(s) 56, 240, 292, 846
Csp6I GTAC 3 cut(s) 407, 431, 625
CviAII CATG 9 cut(s) 18, 90, 322, 383, 410, 434, 622, 671, 775
CviQI GTAC 3 cut(s) 407, 431, 625
DdeI CTNAG 4 cut(s) 54, 969, 992, 1014
DpnI GATC 3 cut(s) 95, 660, 891
DpnII GATC 3 cut(s) 93, 658, 889
DraIII CACNNNGTG 1 cut(s) 1065
EaeI YGGCCR 2 cut(s) 23, 679
EagI CGGCCG 1 cut(s) 23
Eam1104I CTCTTC 3 cut(s) 297, 509, 818
EarI CTCTTC 3 cut(s) 297, 509, 818
EclXI CGGCCG 1 cut(s) 23
Eco130I CCWWGG 2 cut(s) 376, 417
Eco47I GGWCC 1 cut(s) 665
Eco52I CGGCCG 1 cut(s) 23
Eco57I CTGAAG 2 cut(s) 726, 957
EcoRI GAATTC 1 cut(s) 1050
EcoRII CCWGG 2 cut(s) 661, 892
EcoT14I CCWWGG 2 cut(s) 376, 417
EcoT22I ATGCAT 1 cut(s) 415
ErhI CCWWGG 2 cut(s) 376, 417
FaeI CATG 9 cut(s) 21, 93, 325, 386, 413, 437, 625, 674, 778
FatI CATG 9 cut(s) 17, 89, 321, 382, 409, 433, 621, 670, 774
FauNDI CATATG 2 cut(s) 696, 922
Fnu4HI GCNGC 3 cut(s) 23, 26, 758
FokI GGATG 6 cut(s) 400, 845, 866, 936, 986, 1093
Fsp4HI GCNGC 3 cut(s) 23, 26, 758
FspBI CTAG 2 cut(s) 186, 216
GluI GCNGC 3 cut(s) 23, 26, 758
HaeIII GGCC 4 cut(s) 25, 381, 681, 823
HapII CCGG 4 cut(s) 336, 442, 682, 727
HgaI GACGC 4 cut(s) 56, 240, 292, 846
Hin1I GRCGYC 1 cut(s) 284
Hin1II CATG 9 cut(s) 21, 93, 325, 386, 413, 437, 625, 674, 778
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HinfI GANTC 4 cut(s) 208, 274, 331, 900
HpaII CCGG 4 cut(s) 336, 442, 682, 727
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 5 cut(s) 473, 627, 712, 737, 899
Hpy188I TCNGA 9 cut(s) 129, 153, 256, 574, 853, 885, 937, 965, 1056
Hpy188III TCNNGA 5 cut(s) 280, 727, 775, 971, 1121
Hpy8I GTNNAC 5 cut(s) 473, 627, 712, 737, 899
Hpy99I CGWCG 1 cut(s) 256
HpyAV CCTTC 5 cut(s) 198, 724, 883, 932, 998
HpyCH4III ACNGT 5 cut(s) 46, 400, 741, 788, 1092
HpyCH4IV ACGT 2 cut(s) 633, 1004
HpyCH4V TGCA 7 cut(s) 101, 171, 413, 533, 582, 592, 819
HpyF10VI GCNNNNNNNGC 1 cut(s) 865
HpyF3I CTNAG 4 cut(s) 54, 969, 992, 1014
HpySE526I ACGT 2 cut(s) 633, 1004
Hsp92I GRCGYC 1 cut(s) 284
Hsp92II CATG 9 cut(s) 21, 93, 325, 386, 413, 437, 625, 674, 778
Kpn2I TCCGGA 1 cut(s) 726
Kzo9I GATC 3 cut(s) 93, 658, 889
LguI GCTCTTC 1 cut(s) 818
LmnI GCTCC 2 cut(s) 229, 717
LweI GCATC 2 cut(s) 422, 958
MaeI CTAG 2 cut(s) 186, 216
MaeII ACGT 2 cut(s) 633, 1004
MaeIII GTNAC 2 cut(s) 916, 976
MalI GATC 3 cut(s) 95, 660, 891
MboI GATC 3 cut(s) 93, 658, 889
MboII GAAGA 9 cut(s) 93, 174, 284, 364, 398, 526, 805, 868, 1049
MfeI CAATTG 1 cut(s) 768
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 1 cut(s) 1022
MluCI AATT 9 cut(s) 312, 367, 392, 491, 721, 768, 907, 1050, 1156
MlyI GAGTC 3 cut(s) 202, 325, 894
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 7 cut(s) 175, 219, 250, 297, 300, 346, 499
Mph1103I ATGCAT 1 cut(s) 415
MroI TCCGGA 1 cut(s) 726
MroXI GAANNNNTTC 1 cut(s) 493
MseI TTAA 1 cut(s) 753
MspI CCGG 4 cut(s) 336, 442, 682, 727
MspR9I CCNGG 3 cut(s) 336, 663, 894
MunI CAATTG 1 cut(s) 768
MvaI CCWGG 2 cut(s) 663, 894
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 1 cut(s) 865
NciI CCSGG 1 cut(s) 336
NdeI CATATG 2 cut(s) 696, 922
NdeII GATC 3 cut(s) 93, 658, 889
NlaIII CATG 9 cut(s) 21, 93, 325, 386, 413, 437, 625, 674, 778
NlaIV GGNNCC 1 cut(s) 340
NotI GCGGCCGC 1 cut(s) 23
NsiI ATGCAT 1 cut(s) 415
NspI RCATGY 2 cut(s) 413, 437
PagI TCATGA 1 cut(s) 774
PciSI GCTCTTC 1 cut(s) 818
PcsI WCGNNNNNNNCGW 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 493
PfeI GAWTC 1 cut(s) 274
PflFI GACNNNGTC 1 cut(s) 250
PfoI TCCNGGA 1 cut(s) 334
PkrI GCNGC 3 cut(s) 24, 27, 759
PleI GAGTC 3 cut(s) 202, 325, 894
PpsI GAGTC 3 cut(s) 202, 325, 894
Psp6I CCWGG 2 cut(s) 661, 892
PspGI CCWGG 2 cut(s) 661, 892
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 665
PstI CTGCAG 1 cut(s) 821
PsuI RGATCY 1 cut(s) 889
PsyI GACNNNGTC 1 cut(s) 250
RsaI GTAC 3 cut(s) 408, 432, 626
RsaNI GTAC 3 cut(s) 407, 431, 625
SapI GCTCTTC 1 cut(s) 818
SaqAI TTAA 1 cut(s) 753
SatI GCNGC 3 cut(s) 23, 26, 758
Sau3AI GATC 3 cut(s) 93, 658, 889
Sau96I GGNCC 1 cut(s) 665
SchI GAGTC 3 cut(s) 202, 325, 894
ScrFI CCNGG 3 cut(s) 336, 663, 894
SduI GDGCHC 1 cut(s) 1022
SfaNI GCATC 2 cut(s) 422, 958
SfcI CTRYAG 1 cut(s) 817
SinI GGWCC 1 cut(s) 665
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 9 cut(s) 312, 367, 392, 491, 721, 768, 907, 1050, 1156
SsiI CCGC 4 cut(s) 22, 26, 668, 757
SspMI CTAG 2 cut(s) 186, 216
StyD4I CCNGG 3 cut(s) 334, 661, 892
StyI CCWWGG 2 cut(s) 376, 417
TaaI ACNGT 5 cut(s) 46, 400, 741, 788, 1092
TaiI ACGT 2 cut(s) 636, 1007
TaqI TCGA 5 cut(s) 351, 497, 600, 645, 927
TaqII GACCGA 1 cut(s) 251
TasI AATT 9 cut(s) 312, 367, 392, 491, 721, 768, 907, 1050, 1156
TatI WGTACW 3 cut(s) 406, 430, 624
TauI GCSGC 3 cut(s) 25, 28, 760
TfiI GAWTC 1 cut(s) 274
Tru1I TTAA 1 cut(s) 753
Tru9I TTAA 1 cut(s) 753
TscAI CASTG 3 cut(s) 594, 746, 1134
TspDTI ATGAA 5 cut(s) 17, 103, 310, 399, 1097
TspGWI ACGGA 1 cut(s) 1053
TspRI CASTG 3 cut(s) 594, 746, 1134
Tth111I GACNNNGTC 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 665
XapI RAATTY 4 cut(s) 367, 392, 491, 1050
XceI RCATGY 2 cut(s) 413, 437
XmnI GAANNNNTTC 1 cut(s) 493
XspI CTAG 2 cut(s) 186, 216
Zsp2I ATGCAT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.