MD15G1109800.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004591
Physical Location & Seq
Reverse (-)
7690473 .. 7693906
3434 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1109800.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGAGAGGTTCCGTGTTGCCTTCGGTCCAGATGAAAAAGTCATTAGCATGGCGGCCGCAGCTGATGAGAGATATCATGATCATGTCTAAGTCAAGGCCGCGTCCAGCTTTGAGAAGACCAACATGGATTATTGCATTGGTTTTGTTCATCTGTGTTTTTCTGATCCTTGCATACTTCTACCCTCCGAAAAACTCTGCCGCCTGCTATCTGTTTTCTTCTAGTGGTTGCAGTATGTTTGGAAGGACTCCTCCTGGCCCTGCAAGGGAGCTGACCGATGATGAGACTGCGTCCGAGGTTGTGGTTAGGGAAATTCTCAAGACACCTCCTCTTCAATCGAAGAACCCGAAAATTGCTTTCATGTTTTTAACCCCCGGAACTTTACCTTTCGAGGAGTTGTGGGAAAATTTCTTCCAAGGCCACGAAGACAAATTTACCGTCTATGTACATGCATCAAAGCAAAAGCCAGTACATGTGAGCCGGTATTTTGCTGACAGAGACATACATAGTGAACAGGTCGTTTGGGGAAAATTTTCGATGGTTGAGGCAGAGAAGAGACTTTTGGCAAATGCACTTTTAGACCCTGACAACCAACATTTTGTTTTGCTGTCTGATAGTTGCATACCACTGCATAACTTCGATTATGTATATAACTATCTCATGTACACCAACGTCAGCTTTATCGATTGTTTTGTGGATCCTGGTCCACATGGAACTGGCCGGTATTCAGAACATATGTTGCCTGAAGTTGAAAAGAGGGATTTCCGGAAGGGTTCACAGTGGTTCTCTATGAAGCGGCTACACGCAATTGTCATGATGGCAGACAGTCTATACTATACAAAGTTCAAGCTTTACTGCAGGCCAAACATGGACGGGCGTAACTGCTATTCGGATGAGCATTACCTGCCAACCTTCTTTAATATGAAAGATCCTGGTGGACTCGCGAACTGGTCTGTAACATATGTCGATTGGTCTGAAGGGAAGTGGCATCCAAGAGCGTTCAGAACTCAGGATGTTACTTATGACCTCCTTAGGAACATCACGTCCATTGCTGAAAGCACGCATATTACAAGCGAAGACAAGAAGCAAATGCTGACCACACCGTGCTTGTGGAACGGGATGAAGCGACCGTGTTTTCTATTTGCCCGAAAGTTTTATCCAGAAACACTGGGGAAACTGGTTCAGCTTTTCTCCAATTACACAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

403

Amino Acids

46.83

Weight (kDa)

8.96

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 117 - 346 1.6e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 909
AccII CGCG 2 cut(s) 100, 941
AccIII TCCGGA 1 cut(s) 762
AciI CCGC 5 cut(s) 52, 56, 98, 198, 793
AclWI GGATC 4 cut(s) 157, 689, 702, 920
AcoI YGGCCR 2 cut(s) 53, 715
AcsI RAATTY 4 cut(s) 309, 403, 428, 527
AcuI CTGAAG 2 cut(s) 762, 993
AdeI CACNNNGTG 1 cut(s) 1101
AfaI GTAC 3 cut(s) 444, 468, 662
AfiI CCNNNNNNNGG 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 3 cut(s) 332, 749, 844
AjiI CACGTC 1 cut(s) 1041
AjnI CCWGG 3 cut(s) 250, 697, 928
AjuI GAANNNNNNNTTGG 2 cut(s) 542, 574
AloI GAACNNNNNNTCC 2 cut(s) 1025, 1057
AluBI AGCT 6 cut(s) 61, 107, 268, 675, 847, 1183
AluI AGCT 6 cut(s) 61, 107, 268, 675, 847, 1183
Alw26I GTCTC 3 cut(s) 275, 489, 547
AlwI GGATC 4 cut(s) 157, 689, 702, 920
Aor13HI TCCGGA 1 cut(s) 762
AoxI GGCC 6 cut(s) 53, 95, 253, 415, 715, 857
ApeKI GCWGC 1 cut(s) 58
ApoI RAATTY 4 cut(s) 309, 403, 428, 527
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
Asp700I GAANNNNTTC 1 cut(s) 529
AspS9I GGNCC 3 cut(s) 25, 254, 701
AsuC2I CCSGG 1 cut(s) 372
AvaII GGWCC 2 cut(s) 25, 701
AxyI CCTNAGG 1 cut(s) 1028
BamHI GGATCC 1 cut(s) 694
BbsI GAAGAC 3 cut(s) 121, 429, 1080
BbvI GCAGC 1 cut(s) 70
BccI CCATC 2 cut(s) 529, 808
BciT130I CCWGG 3 cut(s) 252, 699, 930
BclI TGATCA 1 cut(s) 78
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 3 cut(s) 275, 489, 547
BfaI CTAG 1 cut(s) 219
BfmI CTRYAG 1 cut(s) 853
BfuAI ACCTGC 1 cut(s) 909
BisI GCNGC 6 cut(s) 53, 56, 59, 98, 198, 794
BlsI GCNGC 6 cut(s) 54, 57, 60, 99, 199, 795
Bme1390I CCNGG 4 cut(s) 252, 372, 699, 930
Bme18I GGWCC 2 cut(s) 25, 701
BmgBI CACGTC 1 cut(s) 1041
BmgT120I GGNCC 3 cut(s) 25, 254, 701
BmiI GGNNCC 2 cut(s) 10, 696
BmrFI CCNGG 4 cut(s) 252, 372, 699, 930
BmrI ACTGGG 1 cut(s) 1175
BmsI GCATC 2 cut(s) 458, 994
BmuI ACTGGG 1 cut(s) 1175
BpiI GAAGAC 3 cut(s) 121, 429, 1080
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 372
Bsa29I ATCGAT 1 cut(s) 681
BsaJI CCNNGG 3 cut(s) 291, 370, 412
BsaWI WCCGGW 1 cut(s) 762
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse118I RCCGGY 2 cut(s) 477, 717
Bse1I ACTGG 5 cut(s) 464, 718, 950, 1170, 1179
Bse21I CCTNAGG 1 cut(s) 1028
Bse3DI GCAATG 1 cut(s) 1044
BseAI TCCGGA 1 cut(s) 762
BseBI CCWGG 3 cut(s) 252, 699, 930
BseCI ATCGAT 1 cut(s) 681
BseDI CCNNGG 3 cut(s) 291, 370, 412
BseGI GGATG 4 cut(s) 895, 985, 1015, 1122
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 1 cut(s) 1019
BseNI ACTGG 5 cut(s) 464, 718, 950, 1170, 1179
BseRI GAGGAG 3 cut(s) 237, 315, 404
BseX3I CGGCCG 1 cut(s) 53
BseXI GCAGC 1 cut(s) 70
Bsh1236I CGCG 2 cut(s) 100, 941
Bsh1285I CGRYCG 2 cut(s) 56, 1127
BshFI GGCC 6 cut(s) 55, 97, 255, 417, 717, 859
BshVI ATCGAT 1 cut(s) 681
BsiEI CGRYCG 2 cut(s) 56, 1127
BsiSI CCGG 4 cut(s) 372, 478, 718, 763
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 3 cut(s) 275, 489, 547
BsnI GGCC 6 cut(s) 55, 97, 255, 417, 717, 859
Bsp13I TCCGGA 1 cut(s) 762
Bsp1407I TGTACA 2 cut(s) 442, 660
Bsp143I GATC 4 cut(s) 78, 162, 694, 925
Bsp68I TCGCGA 1 cut(s) 941
BspACI CCGC 5 cut(s) 52, 56, 98, 198, 793
BspANI GGCC 6 cut(s) 55, 97, 255, 417, 717, 859
BspCNI CTCAG 1 cut(s) 1018
BspDI ATCGAT 1 cut(s) 681
BspEI TCCGGA 1 cut(s) 762
BspFNI CGCG 2 cut(s) 100, 941
BspHI TCATGA 2 cut(s) 75, 810
BspLI GGNNCC 2 cut(s) 10, 696
BspMAI CTGCAG 1 cut(s) 857
BspMI ACCTGC 1 cut(s) 909
BspPI GGATC 4 cut(s) 157, 689, 702, 920
BsrDI GCAATG 1 cut(s) 1044
BsrFI RCCGGY 2 cut(s) 477, 717
BsrGI TGTACA 2 cut(s) 442, 660
BsrI ACTGG 5 cut(s) 464, 718, 950, 1170, 1179
BssAI RCCGGY 2 cut(s) 477, 717
BssECI CCNNGG 3 cut(s) 291, 370, 412
BssMI GATC 4 cut(s) 78, 162, 694, 925
BssT1I CCWWGG 1 cut(s) 412
Bst2UI CCWGG 3 cut(s) 252, 699, 930
Bst4CI ACNGT 5 cut(s) 436, 777, 824, 1101, 1128
Bst6I CTCTTC 2 cut(s) 333, 545
BstAPI GCANNNNNTGC 1 cut(s) 901
BstAUI TGTACA 2 cut(s) 442, 660
BstC8I GCNNGC 3 cut(s) 202, 857, 1058
BstDEI CTNAG 3 cut(s) 87, 1005, 1028
BstF5I GGATG 4 cut(s) 895, 985, 1015, 1122
BstFNI CGCG 2 cut(s) 100, 941
BstKTI GATC 4 cut(s) 81, 165, 697, 928
BstMAI GTCTC 3 cut(s) 275, 489, 547
BstMBI GATC 4 cut(s) 78, 162, 694, 925
BstMCI CGRYCG 2 cut(s) 56, 1127
BstMWI GCNNNNNNNGC 2 cut(s) 58, 901
BstNI CCWGG 3 cut(s) 252, 699, 930
BstNSI RCATGY 2 cut(s) 449, 473
BstSCI CCNGG 4 cut(s) 250, 370, 697, 928
BstSFI CTRYAG 1 cut(s) 853
BstUI CGCG 2 cut(s) 100, 941
BstV1I GCAGC 1 cut(s) 70
BstV2I GAAGAC 3 cut(s) 121, 429, 1080
BstX2I RGATCY 2 cut(s) 694, 925
BstYI RGATCY 2 cut(s) 694, 925
BstZI CGGCCG 1 cut(s) 53
Bsu15I ATCGAT 1 cut(s) 681
Bsu36I CCTNAGG 1 cut(s) 1028
BsuRI GGCC 6 cut(s) 55, 97, 255, 417, 717, 859
BsuTUI ATCGAT 1 cut(s) 681
BtrI CACGTC 1 cut(s) 1041
BtsCI GGATG 4 cut(s) 895, 985, 1015, 1122
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 3 cut(s) 623, 782, 1163
BtuMI TCGCGA 1 cut(s) 941
BveI ACCTGC 1 cut(s) 909
Cac8I GCNNGC 3 cut(s) 202, 857, 1058
CciI TCATGA 2 cut(s) 75, 810
CciNI GCGGCCGC 1 cut(s) 53
Cfr10I RCCGGY 2 cut(s) 477, 717
Cfr13I GGNCC 3 cut(s) 25, 254, 701
ClaI ATCGAT 1 cut(s) 681
CseI GACGC 2 cut(s) 89, 276
Csp6I GTAC 3 cut(s) 443, 467, 661
CviQI GTAC 3 cut(s) 443, 467, 661
DdeI CTNAG 3 cut(s) 87, 1005, 1028
DpnI GATC 4 cut(s) 80, 164, 696, 927
DpnII GATC 4 cut(s) 78, 162, 694, 925
DraIII CACNNNGTG 1 cut(s) 1101
EaeI YGGCCR 2 cut(s) 53, 715
EagI CGGCCG 1 cut(s) 53
Eam1104I CTCTTC 2 cut(s) 333, 545
EarI CTCTTC 2 cut(s) 333, 545
EclXI CGGCCG 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 412
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 25, 701
Eco52I CGGCCG 1 cut(s) 53
Eco57I CTGAAG 2 cut(s) 762, 993
Eco81I CCTNAGG 1 cut(s) 1028
EcoRII CCWGG 3 cut(s) 250, 697, 928
EcoRV GATATC 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 412
EcoT22I ATGCAT 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 412
FauNDI CATATG 2 cut(s) 732, 958
FbaI TGATCA 1 cut(s) 78
Fnu4HI GCNGC 6 cut(s) 53, 56, 59, 98, 198, 794
FokI GGATG 4 cut(s) 902, 972, 1022, 1129
Fsp4HI GCNGC 6 cut(s) 53, 56, 59, 98, 198, 794
FspBI CTAG 1 cut(s) 219
GluI GCNGC 6 cut(s) 53, 56, 59, 98, 198, 794
HaeIII GGCC 6 cut(s) 55, 97, 255, 417, 717, 859
HapII CCGG 4 cut(s) 372, 478, 718, 763
HgaI GACGC 2 cut(s) 89, 276
HindIII AAGCTT 1 cut(s) 845
HinfI GANTC 2 cut(s) 244, 936
HpaII CCGG 4 cut(s) 372, 478, 718, 763
Hpy166II GTNNAC 5 cut(s) 509, 663, 704, 773, 935
Hpy188I TCNGA 8 cut(s) 162, 186, 292, 610, 727, 889, 973, 1001
Hpy188III TCNNGA 8 cut(s) 28, 76, 316, 763, 811, 940, 1007, 1157
Hpy8I GTNNAC 5 cut(s) 509, 663, 704, 773, 935
HpyAV CCTTC 5 cut(s) 30, 234, 760, 919, 968
HpyCH4III ACNGT 5 cut(s) 436, 777, 824, 1101, 1128
HpyCH4IV ACGT 2 cut(s) 669, 1040
HpyCH4V TGCA 9 cut(s) 134, 170, 228, 260, 449, 569, 618, 628, 855
HpyF10VI GCNNNNNNNGC 2 cut(s) 58, 901
HpyF3I CTNAG 3 cut(s) 87, 1005, 1028
HpySE526I ACGT 2 cut(s) 669, 1040
Kpn2I TCCGGA 1 cut(s) 762
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 4 cut(s) 78, 162, 694, 925
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 2 cut(s) 458, 994
MaeI CTAG 1 cut(s) 219
MaeII ACGT 2 cut(s) 669, 1040
MaeIII GTNAC 3 cut(s) 875, 952, 1012
MalI GATC 4 cut(s) 80, 164, 696, 927
MboI GATC 4 cut(s) 78, 162, 694, 925
MboII GAAGA 8 cut(s) 126, 207, 320, 349, 400, 434, 562, 1085
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 2 cut(s) 694, 925
MluCI AATT 7 cut(s) 309, 348, 403, 428, 527, 804, 1192
MlyI GAGTC 2 cut(s) 238, 930
MnlI CCTC 9 cut(s) 192, 258, 286, 333, 336, 382, 535, 747, 1034
Mph1103I ATGCAT 1 cut(s) 451
MroI TCCGGA 1 cut(s) 762
MroXI GAANNNNTTC 1 cut(s) 529
MseI TTAA 2 cut(s) 365, 915
MslI CAYNNNNRTG 2 cut(s) 46, 80
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 4 cut(s) 372, 478, 718, 763
MspR9I CCNGG 4 cut(s) 252, 372, 699, 930
MunI CAATTG 1 cut(s) 804
MvaI CCWGG 3 cut(s) 252, 699, 930
MvnI CGCG 2 cut(s) 100, 941
MwoI GCNNNNNNNGC 2 cut(s) 58, 901
NciI CCSGG 1 cut(s) 372
NdeI CATATG 2 cut(s) 732, 958
NdeII GATC 4 cut(s) 78, 162, 694, 925
NlaIV GGNNCC 2 cut(s) 10, 696
NotI GCGGCCGC 1 cut(s) 53
NruI TCGCGA 1 cut(s) 941
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 2 cut(s) 449, 473
PagI TCATGA 2 cut(s) 75, 810
PciI ACATGT 1 cut(s) 469
PcsI WCGNNNNNNNCGW 1 cut(s) 341
PdmI GAANNNNTTC 1 cut(s) 529
PflFI GACNNNGTC 1 cut(s) 286
PkrI GCNGC 6 cut(s) 54, 57, 60, 99, 199, 795
PleI GAGTC 2 cut(s) 238, 930
PpsI GAGTC 2 cut(s) 238, 930
PscI ACATGT 1 cut(s) 469
Psp6I CCWGG 3 cut(s) 250, 697, 928
PspGI CCWGG 3 cut(s) 250, 697, 928
PspN4I GGNNCC 2 cut(s) 10, 696
PspPI GGNCC 3 cut(s) 25, 254, 701
PstI CTGCAG 1 cut(s) 857
PsuI RGATCY 2 cut(s) 694, 925
PsyI GACNNNGTC 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 61
RruI TCGCGA 1 cut(s) 941
RsaI GTAC 3 cut(s) 444, 468, 662
RsaNI GTAC 3 cut(s) 443, 467, 661
RseI CAYNNNNRTG 2 cut(s) 46, 80
SaqAI TTAA 2 cut(s) 365, 915
SatI GCNGC 6 cut(s) 53, 56, 59, 98, 198, 794
Sau3AI GATC 4 cut(s) 78, 162, 694, 925
Sau96I GGNCC 3 cut(s) 25, 254, 701
SchI GAGTC 2 cut(s) 238, 930
ScrFI CCNGG 4 cut(s) 252, 372, 699, 930
SfaNI GCATC 2 cut(s) 458, 994
SfcI CTRYAG 1 cut(s) 853
SinI GGWCC 2 cut(s) 25, 701
SmiMI CAYNNNNRTG 2 cut(s) 46, 80
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 7 cut(s) 309, 348, 403, 428, 527, 804, 1192
SsiI CCGC 5 cut(s) 52, 56, 98, 198, 793
SspMI CTAG 1 cut(s) 219
StyD4I CCNGG 4 cut(s) 250, 370, 697, 928
StyI CCWWGG 1 cut(s) 412
TaaI ACNGT 5 cut(s) 436, 777, 824, 1101, 1128
TaiI ACGT 2 cut(s) 672, 1043
TaqI TCGA 6 cut(s) 335, 387, 533, 636, 681, 963
TaqII GACCGA 2 cut(s) 13, 287
TasI AATT 7 cut(s) 309, 348, 403, 428, 527, 804, 1192
TatI WGTACW 3 cut(s) 442, 466, 660
TauI GCSGC 5 cut(s) 55, 58, 100, 200, 796
Tru1I TTAA 2 cut(s) 365, 915
Tru9I TTAA 2 cut(s) 365, 915
TscAI CASTG 3 cut(s) 630, 782, 1170
TseI GCWGC 1 cut(s) 58
TspDTI ATGAA 6 cut(s) 47, 136, 346, 803, 935, 1133
TspRI CASTG 3 cut(s) 630, 782, 1170
Tth111I GACNNNGTC 1 cut(s) 286
VpaK11BI GGWCC 2 cut(s) 25, 701
XapI RAATTY 4 cut(s) 309, 403, 428, 527
XceI RCATGY 2 cut(s) 449, 473
XmnI GAANNNNTTC 1 cut(s) 529
XspI CTAG 1 cut(s) 219
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.