FvH4_2g30700

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004591
Physical Location & Seq
Forward (+)
23724012 .. 23727987
3976 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30700.t1

Sequence Viewer

Length: 1200 bp
ATGTTGCCTTCAACCCAATTGAAAAAAGGTGGTGCATGGAGGCCACAATTGATGAAAGACATAATGGTCATGTCCAAGTCAAGGTCTCGCTCACAATTGAAAAGACAAAAATGGATCATTGTATTGGCTTGCGTCGTCTCCGTTTTTCTGATCACTGCTTATTTCTATCCCTCGCAAGGCTCTTCCACATGCTATCTCTTTTCTTCTCCTAATTGTAGTGGGATGTTTGGAAAGAGTCCACCTACTCCTACAAGGGAACTAACTGATGATGAGACTGCATCTGAAGTTGTAGTGAAGGAAATTCTCAAGACGCCTCCTCTTCAGTCTAAGAACCCAAAAATCGCTTTCATGTTTTTGACTCCAGGCACTTTACCTTTCGAGGAATTATGGGAAAATTTCTTTCAGGGCCACGAGGACAAATTCAGTGTCTATGTACATGCATCGAAGCAGAAACCAATACATGTGAGCCGCTATTTTGTTGATAGGGACATACATAGTGAACAGGTTGTTTGGGGGAAGTTTTCAATGGTTGAGGCAGAAAAGAGACTCTTAGCTAATGCGCTTTTAGACCCTGATAACCAACACTTTGTTCTGCTGTCTGACAGCTGTATACCACTGCATAGCTTTGATTATGTATATAACTATCTCATGTACACGAATGTCAGCTTTATAGATTGTTTTGTGGATCCTGGTCCGCATGGAACTGGACGGTATTCTGAGCATATGATGCCTGAAGTTGAAAAGCATGAATTTCGAAAGGGTTCACAGTGGTTCACTATGAAGAGGCAACATGCAATTGTCATGATGGCAGACAGTCTATACTATACCAAGTTCAAGCTTCACTGCAGGCCGAACATGGATGGGCGCAACTGCTATTCGGACGAGCATTACCTGCCAACCTTCTTTCGGATGAAAGATCCTGCTGGTCTTGCAAATTGGTCGGTAACATTTGTTGATTGGTCTGAAGGAAAATGGCATCCAAGAGCATTTAGGACTCAGGATGTTAGTTATGATCTTCTTAGGAACATCACGTCCATTGCAGAGAGCACACATGTGACAAGTGATGAATCGAGAACACTATTGAGCAGTCCTTGCATGTGGAATGGGATGAAGCGGCCCTGTTTCTTATTTGCCAGAAAGTTCTATCCTGAAACTCTGGGGAAACTGATACACCTTTTCTCCAATTACACAAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

400

Amino Acids

46.34

Weight (kDa)

8.79

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 114 - 343 1.1e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 900
AccI GTMKAC 1 cut(s) 610
AciI CCGC 3 cut(s) 469, 695, 1114
AclWI GGATC 4 cut(s) 122, 680, 693, 911
AcsI RAATTY 4 cut(s) 300, 394, 419, 749
AcuI CTGAAG 4 cut(s) 303, 305, 753, 984
AcyI GRCGYC 1 cut(s) 311
AfaI GTAC 2 cut(s) 435, 653
AfiI CCNNNNNNNGG 3 cut(s) 81, 176, 906
AflIII ACRYGT 2 cut(s) 460, 1051
AgsI TTSAA 6 cut(s) 12, 22, 100, 525, 740, 835
AjiI CACGTC 1 cut(s) 1032
AjnI CCWGG 2 cut(s) 361, 688
AjuI GAANNNNNNNTTGG 2 cut(s) 573, 605
AleI CACNNNNGTG 1 cut(s) 1052
AloI GAACNNNNNNTCC 2 cut(s) 1016, 1048
AluBI AGCT 5 cut(s) 554, 606, 624, 666, 838
AluI AGCT 5 cut(s) 554, 606, 624, 666, 838
Alw21I GWGCWC 1 cut(s) 1049
Alw26I GTCTC 4 cut(s) 90, 142, 266, 538
AlwI GGATC 4 cut(s) 122, 680, 693, 911
AoxI GGCC 4 cut(s) 41, 406, 848, 1115
ApoI RAATTY 4 cut(s) 300, 394, 419, 749
ArsI GACNNNNNNTTYG 2 cut(s) 411, 443
Asp700I GAANNNNTTC 1 cut(s) 760
AspLEI GCGC 2 cut(s) 562, 867
AspS9I GGNCC 3 cut(s) 406, 692, 1116
AsuII TTCGAA 1 cut(s) 754
AvaII GGWCC 1 cut(s) 692
BamHI GGATCC 1 cut(s) 685
BauI CACGAG 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 1049
BccI CCATC 2 cut(s) 799, 854
BcgI CGANNNNNNTGC 4 cut(s) 734, 768, 921, 955
BciT130I CCWGG 2 cut(s) 363, 690
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 4 cut(s) 90, 142, 266, 538
BfmI CTRYAG 1 cut(s) 844
BfuAI ACCTGC 1 cut(s) 900
BisI GCNGC 2 cut(s) 469, 1115
BlsI GCNGC 2 cut(s) 470, 1116
Bme1390I CCNGG 2 cut(s) 363, 690
Bme18I GGWCC 1 cut(s) 692
BmgBI CACGTC 1 cut(s) 1032
BmgT120I GGNCC 3 cut(s) 406, 692, 1116
BmiI GGNNCC 1 cut(s) 687
BmrFI CCNGG 2 cut(s) 363, 690
BmsI GCATC 4 cut(s) 287, 449, 717, 985
BpmI CTGGAG 1 cut(s) 345
Bpu14I TTCGAA 1 cut(s) 754
BpuEI CTTGAG 1 cut(s) 290
BsaHI GRCGYC 1 cut(s) 311
BsaI GGTCTC 1 cut(s) 90
BsaXI ACNNNNNCTCC 2 cut(s) 1163, 1193
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 176, 906
Bse1I ACTGG 1 cut(s) 709
Bse3DI GCAATG 1 cut(s) 1035
BseBI CCWGG 2 cut(s) 363, 690
BseGI GGATG 6 cut(s) 228, 865, 915, 976, 1006, 1113
BseLI CCNNNNNNNGG 3 cut(s) 81, 176, 906
BseMI GCAATG 1 cut(s) 1035
BseMII CTCAG 2 cut(s) 708, 1010
BseNI ACTGG 1 cut(s) 709
BseRI GAGGAG 1 cut(s) 306
BshFI GGCC 4 cut(s) 43, 408, 850, 1117
BsiHKAI GWGCWC 1 cut(s) 1049
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 3 cut(s) 81, 176, 906
BsmAI GTCTC 4 cut(s) 90, 142, 266, 538
BsmBI CGTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 4 cut(s) 43, 408, 850, 1117
Bso31I GGTCTC 1 cut(s) 90
Bsp119I TTCGAA 1 cut(s) 754
Bsp1286I GDGCHC 1 cut(s) 1049
Bsp1407I TGTACA 2 cut(s) 433, 651
Bsp143I GATC 5 cut(s) 114, 150, 685, 916, 1012
BspACI CCGC 3 cut(s) 469, 695, 1114
BspANI GGCC 4 cut(s) 43, 408, 850, 1117
BspCNI CTCAG 2 cut(s) 709, 1009
BspHI TCATGA 1 cut(s) 801
BspLI GGNNCC 1 cut(s) 687
BspMAI CTGCAG 1 cut(s) 848
BspMI ACCTGC 1 cut(s) 900
BspPI GGATC 4 cut(s) 122, 680, 693, 911
BspQI GCTCTTC 1 cut(s) 187
BspT104I TTCGAA 1 cut(s) 754
BspTNI GGTCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 1035
BsrGI TGTACA 2 cut(s) 433, 651
BsrI ACTGG 1 cut(s) 709
BssMI GATC 5 cut(s) 114, 150, 685, 916, 1012
BssNAI GTATAC 1 cut(s) 611
BssNI GRCGYC 1 cut(s) 311
BssSI CACGAG 1 cut(s) 410
Bst1107I GTATAC 1 cut(s) 611
Bst2BI CACGAG 1 cut(s) 410
Bst2UI CCWGG 2 cut(s) 363, 690
Bst4CI ACNGT 3 cut(s) 711, 768, 815
Bst6I CTCTTC 3 cut(s) 187, 324, 776
BstACI GRCGYC 1 cut(s) 311
BstAPI GCANNNNNTGC 3 cut(s) 727, 892, 1092
BstAUI TGTACA 2 cut(s) 433, 651
BstBI TTCGAA 1 cut(s) 754
BstC8I GCNNGC 2 cut(s) 130, 848
BstDEI CTNAG 5 cut(s) 327, 550, 717, 996, 1019
BstF5I GGATG 6 cut(s) 228, 865, 915, 976, 1006, 1113
BstHHI GCGC 2 cut(s) 562, 867
BstKTI GATC 5 cut(s) 117, 153, 688, 919, 1015
BstMAI GTCTC 4 cut(s) 90, 142, 266, 538
BstMBI GATC 5 cut(s) 114, 150, 685, 916, 1012
BstMWI GCNNNNNNNGC 4 cut(s) 727, 892, 929, 1092
BstNI CCWGG 2 cut(s) 363, 690
BstNSI RCATGY 6 cut(s) 192, 440, 464, 794, 1055, 1099
BstSCI CCNGG 2 cut(s) 361, 688
BstSFI CTRYAG 1 cut(s) 844
BstX2I RGATCY 2 cut(s) 685, 916
BstYI RGATCY 2 cut(s) 685, 916
BstZ17I GTATAC 1 cut(s) 611
BsuRI GGCC 4 cut(s) 43, 408, 850, 1117
BtrI CACGTC 1 cut(s) 1032
BtsCI GGATG 6 cut(s) 228, 865, 915, 976, 1006, 1113
BtsI GCAGTG 3 cut(s) 153, 614, 841
BtsIMutI CAGTG 5 cut(s) 153, 430, 614, 773, 841
BveI ACCTGC 1 cut(s) 900
Cac8I GCNNGC 2 cut(s) 130, 848
CciI TCATGA 1 cut(s) 801
CfoI GCGC 2 cut(s) 562, 867
Cfr13I GGNCC 3 cut(s) 406, 692, 1116
CseI GACGC 2 cut(s) 121, 319
Csp6I GTAC 2 cut(s) 434, 652
CviQI GTAC 2 cut(s) 434, 652
DdeI CTNAG 5 cut(s) 327, 550, 717, 996, 1019
DpnI GATC 5 cut(s) 116, 152, 687, 918, 1014
DpnII GATC 5 cut(s) 114, 150, 685, 916, 1012
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
Eam1104I CTCTTC 3 cut(s) 187, 324, 776
EarI CTCTTC 3 cut(s) 187, 324, 776
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 692
Eco57I CTGAAG 4 cut(s) 303, 305, 753, 984
EcoRII CCWGG 2 cut(s) 361, 688
EcoT22I ATGCAT 1 cut(s) 442
Esp3I CGTCTC 1 cut(s) 142
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FaqI GGGAC 1 cut(s) 500
FauNDI CATATG 1 cut(s) 723
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 1 cut(s) 610
Fnu4HI GCNGC 2 cut(s) 469, 1115
FokI GGATG 6 cut(s) 235, 872, 922, 963, 1013, 1120
Fsp4HI GCNGC 2 cut(s) 469, 1115
GlaI GCGC 2 cut(s) 561, 866
GluI GCNGC 2 cut(s) 469, 1115
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 4 cut(s) 43, 408, 850, 1117
HgaI GACGC 2 cut(s) 121, 319
HhaI GCGC 2 cut(s) 562, 867
Hin1I GRCGYC 1 cut(s) 311
Hin6I GCGC 2 cut(s) 560, 865
HinP1I GCGC 2 cut(s) 560, 865
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 5 cut(s) 235, 358, 546, 994, 1067
Hpy166II GTNNAC 6 cut(s) 239, 500, 611, 654, 764, 774
Hpy188I TCNGA 7 cut(s) 150, 283, 601, 718, 880, 909, 964
Hpy188III TCNNGA 5 cut(s) 307, 802, 998, 1071, 1148
Hpy8I GTNNAC 6 cut(s) 239, 500, 611, 654, 764, 774
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 4 cut(s) 18, 289, 910, 959
HpyCH4III ACNGT 3 cut(s) 711, 768, 815
HpyCH4IV ACGT 1 cut(s) 1031
HpyCH4V TGCA 9 cut(s) 35, 278, 440, 619, 794, 846, 932, 1040, 1095
HpyF10VI GCNNNNNNNGC 4 cut(s) 727, 892, 929, 1092
HpyF3I CTNAG 5 cut(s) 327, 550, 717, 996, 1019
HpySE526I ACGT 1 cut(s) 1031
Hsp92I GRCGYC 1 cut(s) 311
HspAI GCGC 2 cut(s) 560, 865
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 5 cut(s) 114, 150, 685, 916, 1012
LguI GCTCTTC 1 cut(s) 187
LweI GCATC 4 cut(s) 287, 449, 717, 985
MaeII ACGT 1 cut(s) 1031
MaeIII GTNAC 2 cut(s) 943, 1054
MalI GATC 5 cut(s) 116, 152, 687, 918, 1014
MboI GATC 5 cut(s) 114, 150, 685, 916, 1012
MboII GAAGA 5 cut(s) 174, 195, 311, 793, 1007
MfeI CAATTG 4 cut(s) 17, 47, 95, 795
MflI RGATCY 2 cut(s) 685, 916
MhlI GDGCHC 1 cut(s) 1049
MlyI GAGTC 4 cut(s) 244, 352, 540, 988
MnlI CCTC 8 cut(s) 33, 181, 324, 327, 373, 406, 526, 777
Mph1103I ATGCAT 1 cut(s) 442
MroXI GAANNNNTTC 1 cut(s) 760
MslI CAYNNNNRTG 1 cut(s) 1052
MspA1I CMGCKG 1 cut(s) 606
MspR9I CCNGG 2 cut(s) 363, 690
MunI CAATTG 4 cut(s) 17, 47, 95, 795
MvaI CCWGG 2 cut(s) 363, 690
MwoI GCNNNNNNNGC 4 cut(s) 727, 892, 929, 1092
NdeI CATATG 1 cut(s) 723
NdeII GATC 5 cut(s) 114, 150, 685, 916, 1012
NlaIV GGNNCC 1 cut(s) 687
NmuCI GTSAC 1 cut(s) 1054
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 6 cut(s) 192, 440, 464, 794, 1055, 1099
NspV TTCGAA 1 cut(s) 754
OliI CACNNNNGTG 1 cut(s) 1052
PagI TCATGA 1 cut(s) 801
PciI ACATGT 2 cut(s) 460, 1051
PciSI GCTCTTC 1 cut(s) 187
PdmI GAANNNNTTC 1 cut(s) 760
PfeI GAWTC 1 cut(s) 1067
PkrI GCNGC 2 cut(s) 470, 1116
PleI GAGTC 4 cut(s) 243, 352, 540, 988
PpsI GAGTC 4 cut(s) 243, 352, 540, 988
PscI ACATGT 2 cut(s) 460, 1051
Psp6I CCWGG 2 cut(s) 361, 688
PspGI CCWGG 2 cut(s) 361, 688
PspN4I GGNNCC 1 cut(s) 687
PspPI GGNCC 3 cut(s) 406, 692, 1116
PstI CTGCAG 1 cut(s) 848
PsuI RGATCY 2 cut(s) 685, 916
PvuII CAGCTG 1 cut(s) 606
RsaI GTAC 2 cut(s) 435, 653
RsaNI GTAC 2 cut(s) 434, 652
RseI CAYNNNNRTG 1 cut(s) 1052
SapI GCTCTTC 1 cut(s) 187
SatI GCNGC 2 cut(s) 469, 1115
Sau3AI GATC 5 cut(s) 114, 150, 685, 916, 1012
Sau96I GGNCC 3 cut(s) 406, 692, 1116
SchI GAGTC 4 cut(s) 244, 352, 540, 988
ScrFI CCNGG 2 cut(s) 363, 690
SduI GDGCHC 1 cut(s) 1049
SfaNI GCATC 4 cut(s) 287, 449, 717, 985
SfcI CTRYAG 1 cut(s) 844
SfuI TTCGAA 1 cut(s) 754
SinI GGWCC 1 cut(s) 692
SmiMI CAYNNNNRTG 1 cut(s) 1052
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
SsiI CCGC 3 cut(s) 469, 695, 1114
StyD4I CCNGG 2 cut(s) 361, 688
TaaI ACNGT 3 cut(s) 711, 768, 815
TaiI ACGT 1 cut(s) 1034
TaqI TCGA 4 cut(s) 378, 443, 754, 1070
TatI WGTACW 2 cut(s) 433, 651
TauI GCSGC 2 cut(s) 471, 1117
TfiI GAWTC 1 cut(s) 1067
TscAI CASTG 5 cut(s) 160, 430, 621, 773, 848
TseFI GTSAC 1 cut(s) 1054
Tsp45I GTSAC 1 cut(s) 1054
TspDTI ATGAA 7 cut(s) 68, 337, 762, 794, 926, 1080, 1124
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 5 cut(s) 160, 430, 621, 773, 848
VpaK11BI GGWCC 1 cut(s) 692
XapI RAATTY 4 cut(s) 300, 394, 419, 749
XceI RCATGY 6 cut(s) 192, 440, 464, 794, 1055, 1099
XmiI GTMKAC 1 cut(s) 610
XmnI GAANNNNTTC 1 cut(s) 760
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.