AT4G31750

protein phosphatase 2C 59

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005994
Physical Location & Seq
Reverse (-)
15363708 .. 15367892
4185 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31750.2

Sequence Viewer

Length: 936 bp
ATGGGATATCTGAATTCTGTTTTGTCATCTTCGAGCCAGGTTCACTCCGACGATGGACCTGTTAGCGGTGGTGGCCTCAGTCAGAACGGGAAGTTCAGCTATGGATATGCAAGCTCTCCCGGTAAAAGATCTTCTATGGAGGACTTCTATGAAACTAGGATCGACGGCGTTGAAGGGGAAATAGTTGGTCTATTTGGAGTCTTTGATGGACATGGAGGTGCACGTGCAGCTGAATATGTGAAGCAAAATCTCTTCAGTAACCTGATCAGGCATCCAAAGTTCATCTCTGATACCACAGCTGCAATAGCTGATGCATACAACCAAACAGACTCAGAGTTTCTTAAATCAGAAAATAGTCAGAACAGAGATGCTGGTTCAACGGCGTCAACAGCCATCTTAGTTGGTGACCGTTTACTTGTTGCAAATGTAGGGGACTCTAGAGCTGTAATATGCAGAGGTGGCAATGCTATTGCTGTATCCCGAGATCACAAGCCTGATCAAAGTGACGAGCGCCAAAGAATTGAGGATGCAGGAGGATTTGTCATGTGGGCTGGAACATGGAGAGTTGGAGGAGTTCTTGCTGTTTCTCGTGCATTTGGCGATAGGTTGTTGAAGCAGTATGTTGTTGCTGATCCGGAGATACAGGAGGAAAAAGTTGATAGCTCTCTCGAGTTTCTCATTCTTGCAAGTGATGGTCTCTGGGACGTTGTATCTAACGAGGAAGCCGTAGGCATGATCAAGGCGATAGAAGATCCCGAGGAAGGTGCAAAGAGACTGATGATGGAAGCTTACCAAAGAGGAAGTGCAGACAACATAACTTGTGTCGTCGTACGTTTCTTTTCAGACCAAGCAGGAGGAATAGGTTCCAGCAGCACCAATATCCCAATAGATCATGGTATCGTACCTGACCGAATCTCCGGTGACTCATCAACCTAG

Protein Analysis

311

Amino Acids

33.25

Weight (kDa)

4.67

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 62 - 272 5.1e-66 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 634
AciI CCGC 1 cut(s) 66
AclWI GGATC 3 cut(s) 167, 626, 746
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 238
AcvI CACGTG 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 2 cut(s) 831, 903
AfiI CCNNNNNNNGG 2 cut(s) 65, 761
AgsI TTSAA 3 cut(s) 173, 378, 613
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 8 cut(s) 99, 114, 230, 299, 308, 443, 663, 788
AluI AGCT 8 cut(s) 99, 114, 230, 299, 308, 443, 663, 788
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 2 cut(s) 701, 766
Alw44I GTGCAC 1 cut(s) 219
AlwI GGATC 3 cut(s) 167, 626, 746
Ama87I CYCGRG 3 cut(s) 480, 668, 755
Aor13HI TCCGGA 1 cut(s) 634
AoxI GGCC 1 cut(s) 73
ApaLI GTGCAC 1 cut(s) 219
ApeKI GCWGC 3 cut(s) 227, 299, 870
ApoI RAATTY 1 cut(s) 13
Asp700I GAANNNNTTC 1 cut(s) 862
AspLEI GCGC 1 cut(s) 513
AspS9I GGNCC 1 cut(s) 56
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 416, 932
AvaI CYCGRG 3 cut(s) 480, 668, 755
AvaII GGWCC 1 cut(s) 56
BaeGI GKGCMC 1 cut(s) 223
BarI GAAGNNNNNNTAC 2 cut(s) 115, 147
BauI CACGAG 1 cut(s) 588
BbrPI CACGTG 1 cut(s) 224
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 3 cut(s) 239, 286, 882
BccI CCATC 5 cut(s) 47, 200, 401, 686, 775
BceAI ACGGC 3 cut(s) 181, 396, 710
BcgI CGANNNNNNTGC 2 cut(s) 746, 780
BciT130I CCWGG 1 cut(s) 38
BciVI GTATCC 1 cut(s) 487
BclI TGATCA 3 cut(s) 264, 496, 735
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 2 cut(s) 701, 766
BfaI CTAG 3 cut(s) 156, 438, 934
BfoI RGCGCY 1 cut(s) 514
BfuI GTATCC 1 cut(s) 487
BglII AGATCT 1 cut(s) 128
BisI GCNGC 3 cut(s) 228, 300, 871
BlsI GCNGC 3 cut(s) 229, 301, 872
Bme1390I CCNGG 2 cut(s) 38, 120
Bme18I GGWCC 1 cut(s) 56
BmeT110I CYCGRG 3 cut(s) 480, 668, 755
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 1 cut(s) 865
BmrFI CCNGG 2 cut(s) 38, 120
BmsI GCATC 4 cut(s) 280, 301, 358, 517
BpuMI CCSGG 1 cut(s) 120
BsaAI YACGTR 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 383
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 1 cut(s) 756
BsaWI WCCGGW 2 cut(s) 634, 917
BsaXI ACNNNNNCTCC 4 cut(s) 525, 555, 899, 929
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 761
Bse3DI GCAATG 1 cut(s) 469
BseAI TCCGGA 1 cut(s) 634
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 756
BseGI GGATG 2 cut(s) 271, 532
BseLI CCNNNNNNNGG 2 cut(s) 65, 761
BseMI GCAATG 1 cut(s) 469
BseMII CTCAG 2 cut(s) 91, 345
BseRI GAGGAG 1 cut(s) 585
BseSI GKGCMC 1 cut(s) 223
BseXI GCAGC 3 cut(s) 239, 286, 882
BsgI GTGCAG 2 cut(s) 246, 825
BshFI GGCC 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 3 cut(s) 480, 668, 755
BsiSI CCGG 3 cut(s) 120, 635, 918
BsiWI CGTACG 1 cut(s) 829
BslFI GGGAC 2 cut(s) 446, 716
BslI CCNNNNNNNGG 2 cut(s) 65, 761
BsmAI GTCTC 2 cut(s) 701, 766
BsmFI GGGAC 2 cut(s) 446, 716
BsnI GGCC 1 cut(s) 75
Bso31I GGTCTC 1 cut(s) 701
BsoBI CYCGRG 3 cut(s) 480, 668, 755
Bsp1286I GDGCHC 1 cut(s) 223
Bsp13I TCCGGA 1 cut(s) 634
Bsp143I GATC 9 cut(s) 128, 159, 264, 484, 496, 631, 735, 751, 889
BspACI CCGC 1 cut(s) 66
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 2 cut(s) 90, 344
BspEI TCCGGA 1 cut(s) 634
BspLI GGNNCC 1 cut(s) 865
BspPI GGATC 3 cut(s) 167, 626, 746
BspTNI GGTCTC 1 cut(s) 701
BsrDI GCAATG 1 cut(s) 469
BssECI CCNNGG 1 cut(s) 756
BssMI GATC 9 cut(s) 128, 159, 264, 484, 496, 631, 735, 751, 889
BssNI GRCGYC 1 cut(s) 383
BssSI CACGAG 1 cut(s) 588
Bst2BI CACGAG 1 cut(s) 588
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 257
BstACI GRCGYC 1 cut(s) 383
BstBAI YACGTR 1 cut(s) 224
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 3 cut(s) 77, 331, 397
BstEII GGTNACC 1 cut(s) 404
BstF5I GGATG 2 cut(s) 271, 532
BstH2I RGCGCY 1 cut(s) 514
BstHHI GCGC 1 cut(s) 513
BstKTI GATC 9 cut(s) 131, 162, 267, 487, 499, 634, 738, 754, 892
BstMAI GTCTC 2 cut(s) 701, 766
BstMBI GATC 9 cut(s) 128, 159, 264, 484, 496, 631, 735, 751, 889
BstMWI GCNNNNNNNGC 5 cut(s) 72, 227, 305, 389, 459
BstNI CCWGG 1 cut(s) 38
BstPI GGTNACC 1 cut(s) 404
BstSCI CCNGG 2 cut(s) 36, 118
BstSLI GKGCMC 1 cut(s) 223
BstV1I GCAGC 3 cut(s) 239, 286, 882
BstX2I RGATCY 2 cut(s) 128, 751
BstYI RGATCY 2 cut(s) 128, 751
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 1 cut(s) 75
BtsCI GGATG 2 cut(s) 271, 532
Cac8I GCNNGC 1 cut(s) 112
CfoI GCGC 1 cut(s) 513
Cfr13I GGNCC 1 cut(s) 56
CseI GACGC 1 cut(s) 372
Csp6I GTAC 2 cut(s) 830, 902
CviAII CATG 5 cut(s) 212, 544, 558, 733, 893
CviQI GTAC 2 cut(s) 830, 902
DdeI CTNAG 3 cut(s) 77, 331, 397
DpnI GATC 9 cut(s) 130, 161, 266, 486, 498, 633, 737, 753, 891
DpnII GATC 9 cut(s) 128, 159, 264, 484, 496, 631, 735, 751, 889
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
Eco31I GGTCTC 1 cut(s) 701
Eco32I GATATC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 238
Eco72I CACGTG 1 cut(s) 224
Eco88I CYCGRG 3 cut(s) 480, 668, 755
Eco91I GGTNACC 1 cut(s) 404
EcoO65I GGTNACC 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 36
EcoRV GATATC 1 cut(s) 8
EcoT22I ATGCAT 1 cut(s) 316
FaeI CATG 5 cut(s) 215, 547, 561, 736, 896
FaqI GGGAC 2 cut(s) 446, 716
FatI CATG 5 cut(s) 211, 543, 557, 732, 892
FbaI TGATCA 3 cut(s) 264, 496, 735
Fnu4HI GCNGC 3 cut(s) 228, 300, 871
FokI GGATG 2 cut(s) 258, 539
Fsp4HI GCNGC 3 cut(s) 228, 300, 871
FspBI CTAG 3 cut(s) 156, 438, 934
GlaI GCGC 1 cut(s) 512
GluI GCNGC 3 cut(s) 228, 300, 871
HaeII RGCGCY 1 cut(s) 514
HaeIII GGCC 1 cut(s) 75
HapII CCGG 3 cut(s) 120, 635, 918
HgaI GACGC 1 cut(s) 372
HhaI GCGC 1 cut(s) 513
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 5 cut(s) 215, 547, 561, 736, 896
Hin6I GCGC 1 cut(s) 511
HinP1I GCGC 1 cut(s) 511
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HindIII AAGCTT 1 cut(s) 786
HinfI GANTC 5 cut(s) 198, 329, 434, 912, 923
HpaII CCGG 3 cut(s) 120, 635, 918
HphI GGTGA 2 cut(s) 416, 932
Hpy166II GTNNAC 4 cut(s) 43, 221, 387, 413
Hpy188I TCNGA 8 cut(s) 12, 49, 84, 289, 334, 349, 360, 844
Hpy188III TCNNGA 5 cut(s) 438, 480, 635, 668, 755
Hpy8I GTNNAC 4 cut(s) 43, 221, 387, 413
Hpy99I CGWCG 3 cut(s) 53, 167, 830
HpyAV CCTTC 2 cut(s) 167, 755
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4IV ACGT 3 cut(s) 223, 705, 832
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 227, 305, 389, 459
HpyF3I CTNAG 3 cut(s) 77, 331, 397
HpySE526I ACGT 3 cut(s) 223, 705, 832
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 5 cut(s) 215, 547, 561, 736, 896
HspAI GCGC 1 cut(s) 511
Kpn2I TCCGGA 1 cut(s) 634
Ksp22I TGATCA 3 cut(s) 264, 496, 735
Kzo9I GATC 9 cut(s) 128, 159, 264, 484, 496, 631, 735, 751, 889
Lsp1109I GCAGC 3 cut(s) 239, 286, 882
LweI GCATC 4 cut(s) 280, 301, 358, 517
MaeI CTAG 3 cut(s) 156, 438, 934
MaeII ACGT 3 cut(s) 223, 705, 832
MaeIII GTNAC 4 cut(s) 257, 404, 503, 920
MalI GATC 9 cut(s) 130, 161, 266, 486, 498, 633, 737, 753, 891
MboI GATC 9 cut(s) 128, 159, 264, 484, 496, 631, 735, 751, 889
MboII GAAGA 4 cut(s) 21, 123, 244, 761
MflI RGATCY 2 cut(s) 128, 751
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 2 cut(s) 13, 519
MlyI GAGTC 4 cut(s) 207, 323, 428, 917
MmeI TCCRAC 2 cut(s) 72, 547
Mph1103I ATGCAT 1 cut(s) 316
MroI TCCGGA 1 cut(s) 634
MroXI GAANNNNTTC 1 cut(s) 862
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 216
MspA1I CMGCKG 2 cut(s) 230, 299
MspI CCGG 3 cut(s) 120, 635, 918
MspR9I CCNGG 2 cut(s) 38, 120
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 5 cut(s) 72, 227, 305, 389, 459
NciI CCSGG 1 cut(s) 120
NdeII GATC 9 cut(s) 128, 159, 264, 484, 496, 631, 735, 751, 889
NlaIII CATG 5 cut(s) 215, 547, 561, 736, 896
NlaIV GGNNCC 1 cut(s) 865
NmuCI GTSAC 3 cut(s) 404, 503, 920
NsiI ATGCAT 1 cut(s) 316
PaeR7I CTCGAG 1 cut(s) 668
PcsI WCGNNNNNNNCGW 1 cut(s) 723
PdmI GAANNNNTTC 1 cut(s) 862
PfeI GAWTC 1 cut(s) 912
Pfl23II CGTACG 1 cut(s) 829
PkrI GCNGC 3 cut(s) 229, 301, 872
PleI GAGTC 4 cut(s) 206, 323, 428, 917
PmaCI CACGTG 1 cut(s) 224
PmlI CACGTG 1 cut(s) 224
PpsI GAGTC 4 cut(s) 206, 323, 428, 917
Ppu21I YACGTR 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 36
PspCI CACGTG 1 cut(s) 224
PspEI GGTNACC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 36
PspLI CGTACG 1 cut(s) 829
PspN4I GGNNCC 1 cut(s) 865
PspPI GGNCC 1 cut(s) 56
PsuI RGATCY 2 cut(s) 128, 751
PvuII CAGCTG 2 cut(s) 230, 299
RsaI GTAC 2 cut(s) 831, 903
RsaNI GTAC 2 cut(s) 830, 902
RseI CAYNNNNRTG 1 cut(s) 216
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 3 cut(s) 228, 300, 871
Sau3AI GATC 9 cut(s) 128, 159, 264, 484, 496, 631, 735, 751, 889
Sau96I GGNCC 1 cut(s) 56
SchI GAGTC 4 cut(s) 207, 323, 428, 917
ScrFI CCNGG 2 cut(s) 38, 120
SduI GDGCHC 1 cut(s) 223
SfaNI GCATC 4 cut(s) 280, 301, 358, 517
Sfr274I CTCGAG 1 cut(s) 668
SinI GGWCC 1 cut(s) 56
SlaI CTCGAG 1 cut(s) 668
SmiMI CAYNNNNRTG 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
Sse9I AATT 2 cut(s) 13, 519
SsiI CCGC 1 cut(s) 66
SspMI CTAG 3 cut(s) 156, 438, 934
StyD4I CCNGG 2 cut(s) 36, 118
TaaI ACNGT 1 cut(s) 410
TaiI ACGT 3 cut(s) 226, 708, 835
TaqI TCGA 3 cut(s) 32, 162, 669
TaqII GACCGA 1 cut(s) 924
TasI AATT 2 cut(s) 13, 519
TfiI GAWTC 1 cut(s) 912
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TseFI GTSAC 3 cut(s) 404, 503, 920
TseI GCWGC 3 cut(s) 227, 299, 870
Tsp45I GTSAC 3 cut(s) 404, 503, 920
TspDTI ATGAA 2 cut(s) 165, 271
VneI GTGCAC 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 1 cut(s) 13
XbaI TCTAGA 1 cut(s) 437
XhoI CTCGAG 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 862
XspI CTAG 3 cut(s) 156, 438, 934
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.