Rh6DG397200

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005994
Physical Location & Seq
Reverse (-)
58791963 .. 58796362
4400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG397200.1

Sequence Viewer

Length: 654 bp
ATGGAAGATTTTTATGAGACAAGAATTGATGGTGTTGAGGGAGAAATAGTTGGTCTCTTCGGAGTTTTTGATGGTCATGGGGGTGCACGTGCTGCTGAATATGTCAAGCAAAATCTGTTTAGTAATTTGATCAGGCATCCAAAGTTTATTTCTGACACCAAATCTGCCATAGCTGATGCATACAGCCATACAGACTCTGAATTCCTGAAGTCAGAAAATAACCAGAATAGAGATGCTGGGTCAACCGCTTCCACTGCTATCCTAGTTGGTGACCGTTTGCTTGTCGCAAACGTTGGGGATTCCAGAGCAGTGATATGCAGGGGTGGTAATGCAATAGCTGTTTCAAGAGATCACAAGCCGGACCAAACTGATGAGCGGCAACGGATTGAGGATGCAGGAGGATTTGTAATGTGGGCAGGAACTTGGAGAGTTGGAGGTGTTCTTGCTGTTTCTCGTGCATTTGGAGATAGGCTGTTGAAGCAGTATGTTGTTGCTGATCCAGAAATCCAGGAGGAAAAGATTGACAACAGTCTTGAGTTTCTTATCCTTGCAAGTGATGGACTGTGGGATGTTGTCACCAATGAGGTTTACCATGATTTTCCATTCTTATTACTATTCTATACTTCTTGTCAATGCACTGAGGGTGGAGGGTGA

Protein Analysis

217

Amino Acids

23.84

Weight (kDa)

4.68

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 18 - 199 2.7e-59 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 376
AciI CCGC 2 cut(s) 246, 376
AclI AACGTT 1 cut(s) 291
AclWI GGATC 1 cut(s) 491
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 227
AcvI CACGTG 1 cut(s) 89
AgsI TTSAA 2 cut(s) 345, 478
AjnI CCWGG 1 cut(s) 507
AjuI GAANNNNNNNTTGG 2 cut(s) 133, 165
AluBI AGCT 2 cut(s) 173, 338
AluI AGCT 2 cut(s) 173, 338
Alw21I GWGCWC 1 cut(s) 88
Alw26I GTCTC 2 cut(s) 11, 59
Alw44I GTGCAC 1 cut(s) 84
AlwI GGATC 1 cut(s) 491
AlwNI CAGNNNCTG 1 cut(s) 197
ApaLI GTGCAC 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 361
AsuHPI GGTGA 2 cut(s) 281, 568
AvaII GGWCC 1 cut(s) 361
BaeGI GKGCMC 1 cut(s) 88
BauI CACGAG 1 cut(s) 453
BbrPI CACGTG 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 88
BbvI GCAGC 1 cut(s) 79
BccI CCATC 3 cut(s) 23, 65, 551
BciT130I CCWGG 1 cut(s) 509
BclI TGATCA 1 cut(s) 129
BcoDI GTCTC 2 cut(s) 11, 59
BfaI CTAG 1 cut(s) 263
BisI GCNGC 2 cut(s) 93, 377
BlsI GCNGC 2 cut(s) 94, 378
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 361
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 4 cut(s) 145, 166, 223, 382
BoxI GACNNNNGTC 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 554
BsaAI YACGTR 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 59
BsaXI ACNNNNNCTCC 4 cut(s) 33, 63, 390, 420
BseBI CCWGG 1 cut(s) 509
BseGI GGATG 3 cut(s) 136, 397, 574
BseMII CTCAG 1 cut(s) 630
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 1 cut(s) 79
BseYI CCCAGC 1 cut(s) 236
BsiHKAI GWGCWC 1 cut(s) 88
BsiSI CCGG 1 cut(s) 359
BsmAI GTCTC 2 cut(s) 11, 59
Bso31I GGTCTC 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 88
Bsp143I GATC 3 cut(s) 129, 349, 496
BspACI CCGC 2 cut(s) 246, 376
BspCNI CTCAG 1 cut(s) 631
BspPI GGATC 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 376
BssMI GATC 3 cut(s) 129, 349, 496
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 3 cut(s) 275, 530, 564
Bst6I CTCTTC 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 92
BstBAI YACGTR 1 cut(s) 89
BstDEI CTNAG 1 cut(s) 639
BstEII GGTNACC 1 cut(s) 269
BstF5I GGATG 3 cut(s) 136, 397, 574
BstKTI GATC 3 cut(s) 132, 352, 499
BstMAI GTCTC 2 cut(s) 11, 59
BstMBI GATC 3 cut(s) 129, 349, 496
BstMWI GCNNNNNNNGC 3 cut(s) 92, 254, 478
BstNI CCWGG 1 cut(s) 509
BstPAI GACNNNNGTC 1 cut(s) 528
BstPI GGTNACC 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 79
BtsCI GGATG 3 cut(s) 136, 397, 574
BtsI GCAGTG 2 cut(s) 252, 315
BtsIMutI CAGTG 3 cut(s) 252, 315, 636
CaiI CAGNNNCTG 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 361
CviAII CATG 2 cut(s) 77, 593
CviJI RGCY 5 cut(s) 173, 186, 338, 358, 472
CviKI_1 RGCY 5 cut(s) 173, 186, 338, 358, 472
DdeI CTNAG 1 cut(s) 639
DpnI GATC 3 cut(s) 131, 351, 498
DpnII GATC 3 cut(s) 129, 349, 496
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco31I GGTCTC 1 cut(s) 59
Eco47I GGWCC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 227
Eco72I CACGTG 1 cut(s) 89
Eco91I GGTNACC 1 cut(s) 269
EcoO65I GGTNACC 1 cut(s) 269
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 507
EcoT22I ATGCAT 1 cut(s) 181
FaeI CATG 2 cut(s) 80, 596
FatI CATG 2 cut(s) 76, 592
FbaI TGATCA 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 93, 377
FokI GGATG 3 cut(s) 123, 404, 581
Fsp4HI GCNGC 2 cut(s) 93, 377
FspBI CTAG 1 cut(s) 263
GluI GCNGC 2 cut(s) 93, 377
GsaI CCCAGC 1 cut(s) 240
HapII CCGG 1 cut(s) 359
Hin1II CATG 2 cut(s) 80, 596
HincII GTYRAC 1 cut(s) 243
HindII GTYRAC 1 cut(s) 243
HinfI GANTC 2 cut(s) 194, 299
HpaII CCGG 1 cut(s) 359
HphI GGTGA 2 cut(s) 281, 568
Hpy166II GTNNAC 3 cut(s) 86, 243, 589
Hpy188I TCNGA 4 cut(s) 62, 154, 199, 214
Hpy188III TCNNGA 5 cut(s) 205, 303, 345, 500, 533
Hpy8I GTNNAC 3 cut(s) 86, 243, 589
HpyCH4III ACNGT 3 cut(s) 275, 530, 564
HpyCH4IV ACGT 2 cut(s) 88, 291
HpyCH4V TGCA 8 cut(s) 86, 179, 318, 332, 395, 458, 551, 636
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 254, 478
HpyF3I CTNAG 1 cut(s) 639
HpySE526I ACGT 2 cut(s) 88, 291
Hsp92II CATG 2 cut(s) 80, 596
Ksp22I TGATCA 1 cut(s) 129
Kzo9I GATC 3 cut(s) 129, 349, 496
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 4 cut(s) 145, 166, 223, 382
MaeI CTAG 1 cut(s) 263
MaeII ACGT 2 cut(s) 88, 291
MaeIII GTNAC 2 cut(s) 269, 574
MalI GATC 3 cut(s) 131, 351, 498
MbiI CCGCTC 1 cut(s) 376
MboI GATC 3 cut(s) 129, 349, 496
MboII GAAGA 2 cut(s) 17, 49
MhlI GDGCHC 1 cut(s) 88
MluCI AATT 3 cut(s) 24, 124, 200
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 8 cut(s) 31, 382, 392, 428, 505, 577, 634, 641
Mph1103I ATGCAT 1 cut(s) 181
MslI CAYNNNNRTG 1 cut(s) 81
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 1 cut(s) 509
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 3 cut(s) 92, 254, 478
NdeII GATC 3 cut(s) 129, 349, 496
NlaIII CATG 2 cut(s) 80, 596
NmuCI GTSAC 2 cut(s) 269, 574
NsiI ATGCAT 1 cut(s) 181
PfeI GAWTC 1 cut(s) 299
PfoI TCCNGGA 1 cut(s) 507
PkrI GCNGC 2 cut(s) 94, 378
PleI GAGTC 1 cut(s) 188
PmaCI CACGTG 1 cut(s) 89
PmlI CACGTG 1 cut(s) 89
PpsI GAGTC 1 cut(s) 188
Ppu21I YACGTR 1 cut(s) 89
PshAI GACNNNNGTC 1 cut(s) 528
Psp1406I AACGTT 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 507
PspCI CACGTG 1 cut(s) 89
PspEI GGTNACC 1 cut(s) 269
PspFI CCCAGC 1 cut(s) 236
PspGI CCWGG 1 cut(s) 507
PspPI GGNCC 1 cut(s) 361
PstNI CAGNNNCTG 1 cut(s) 197
RseI CAYNNNNRTG 1 cut(s) 81
SatI GCNGC 2 cut(s) 93, 377
Sau3AI GATC 3 cut(s) 129, 349, 496
Sau96I GGNCC 1 cut(s) 361
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 1 cut(s) 88
SetI ASST 6 cut(s) 91, 175, 294, 340, 439, 588
SfaNI GCATC 4 cut(s) 145, 166, 223, 382
SinI GGWCC 1 cut(s) 361
SmiMI CAYNNNNRTG 1 cut(s) 81
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
Sse9I AATT 3 cut(s) 24, 124, 200
SsiI CCGC 2 cut(s) 246, 376
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 507
TaaI ACNGT 3 cut(s) 275, 530, 564
TaiI ACGT 2 cut(s) 91, 294
TasI AATT 3 cut(s) 24, 124, 200
TauI GCSGC 1 cut(s) 379
TfiI GAWTC 1 cut(s) 299
TscAI CASTG 3 cut(s) 259, 315, 643
TseFI GTSAC 2 cut(s) 269, 574
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 2 cut(s) 269, 574
TspGWI ACGGA 1 cut(s) 397
TspRI CASTG 3 cut(s) 259, 315, 643
VneI GTGCAC 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 361
XapI RAATTY 1 cut(s) 200
XspI CTAG 1 cut(s) 263
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.