AT4G31850

Pentacotripeptide-repeat region of PRORP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014408
Physical Location & Seq
Forward (+)
15402768 .. 15406493
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31850.1

Sequence Viewer

Length: 3339 bp
ATGGTCGCGTTACTCTGCTCTGCGAGTTTGTGTGGTGATATATCAGTTGGTGATGCTTGTCTTGTTAGTAACATCAAAGCAAAATGTCGCGATAATTTGGTTACTGGAGGATTAAAGTTCCACGCTTTGAAGATTGGGTCTCGGAAGAAACACTGGAGAAGGAAGAGTATGAGATGTAGTGTAGTCTCCATGAAAAGTTCTGACTTTTCGGGTTCTATGATAAGAAAGAGCTCAAAACCTGATTTGTCATCGAGTGAAGAAGTAACGAGGGGTTTAAAGTCGTTTCCTGATACTGATTCTTCATTTTCGTATTTCAAATCAGTGGCTGGGAATTTGAATTTAGTTCACACTACTGAGACTTGTAACTACATGCTTGAAGCTTTGAGGGTTGATGGTAAACTTGAGGAAATGGCTTATGTGTTTGATTTGATGCAGAAAAGAATCATAAAGAGGGATACTAATACTTACTTGACTATCTTCAAGAGTCTTTCTGTGAAAGGTGGATTGAAGCAAGCACCTTATGCACTTCGGAAGATGAGAGAATTTGGTTTTGTGTTGAATGCATATTCATACAATGGGTTGATTCATCTTCTGCTTAAGTCTAGGTTTTGCACAGAGGCAATGGAGGTTTATAGAAGAATGATCTTGGAAGGTTTTAGACCTAGTTTGCAGACTTACTCTTCACTTATGGTAGGTTTGGGGAAAAGAAGGGATATTGATTCTGTGATGGGTTTACTTAAAGAGATGGAGACTTTGGGGTTAAAGCCGAATGTTTACACCTTTACGATATGTATTCGGGTGCTTGGTAGGGCAGGGAAGATTAATGAAGCATATGAAATACTGAAGCGGATGGATGATGAAGGATGTGGACCTGATGTAGTTACTTACACTGTTCTTATTGATGCTCTGTGTACTGCTAGGAAGCTCGATTGTGCGAAGGAGGTTTTCGAAAAGATGAAAACAGGAAGACACAAACCAGATCGTGTAACTTACATTACTTTGCTGGACAGGTTTAGTGATAACAGAGATCTGGATTCAGTGAAGCAGTTTTGGAGTGAAATGGAGAAGGATGGTCATGTACCTGATGTTGTAACCTTTACCATTCTCGTGGATGCTCTGTGCAAAGCTGGGAATTTTGGTGAAGCATTTGATACATTGGATGTGATGAGGGACCAAGGTATCTTACCGAACCTTCATACATATAACACATTGATTTGCGGTCTTCTAAGAGTCCACCGGTTGGATGATGCACTTGAACTGTTTGGTAATATGGAGTCTCTGGGCGTGAAGCCCACTGCCTATACGTACATAGTTTTCATTGATTACTATGGAAAGTCTGGAGATTCCGTCAGTGCTCTTGAGACTTTTGAGAAGATGAAAACCAAAGGAATAGCTCCGAACATTGTAGCCTGTAATGCGTCTCTCTACAGTCTAGCTAAAGCGGGAAGGGACCGAGAAGCGAAACAAATCTTCTATGGCTTGAAAGACATAGGACTGGTCCCTGATTCAGTAACTTATAACATGATGATGAAGTGCTACAGTAAGGTTGGGGAGATAGATGAAGCCATCAAGCTGCTTTCTGAGATGATGGAGAATGGTTGTGAACCTGATGTTATTGTTGTTAATTCTTTGATCAATACACTTTACAAAGCTGATAGAGTAGATGAGGCGTGGAAGATGTTTATGAGAATGAAGGAAATGAAGCTGAAGCCTACAGTTGTGACTTACAACACATTGCTCGCAGGTCTAGGCAAAAATGGAAAAATTCAAGAGGCCATTGAGTTGTTCGAGGGGATGGTGCAGAAAGGTTGTCCACCTAACACAATTACTTTCAACACTCTCTTTGATTGCCTTTGCAAAAATGACGAGGTGACTCTAGCCTTGAAGATGTTGTTCAAGATGATGGACATGGGTTGTGTCCCAGATGTTTTTACTTACAATACGATTATCTTTGGTTTGGTGAAGAATGGTCAAGTCAAAGAGGCAATGTGTTTCTTCCACCAGATGAAGAAACTGGTTTACCCTGATTTTGTTACACTCTGCACTCTTCTCCCAGGTGTTGTCAAGGCCAGTTTAATCGAAGATGCTTACAAAATCATCACCAACTTTCTTTACAATTGTGCAGACCAACCGGCTAATCTTTTCTGGGAAGACTTGATAGGCTCTATCTTGGCTGAAGCTGGAATAGACAATGCCGTTTCATTTTCTGAGAGATTGGTAGCTAATGGGATTTGTCGAGATGGTGACTCTATCCTGGTGCCAATAATCAGATATTCTTGTAAACATAATAACGTCTCAGGTGCTCGAACTTTATTCGAGAAATTCACAAAAGATCTTGGTGTCCAACCCAAACTCCCGACTTATAATCTGCTAATCGGCGGGCTTCTTGAAGCTGATATGATTGAAATAGCACAGGACGTGTTCCTACAGGTGAAGAGCACTGGTTGCATCCCAGATGTGGCGACCTACAACTTCTTGCTTGATGCTTATGGAAAATCAGGAAAAATCGACGAACTGTTTGAGCTATACAAAGAAATGTCTACCCATGAGTGCGAAGCAAACACAATTACTCACAACATTGTCATCTCCGGGCTTGTGAAGGCAGGAAATGTGGACGATGCTCTTGATTTATACTACGACCTTATGAGTGATCGGGACTTCTCCCCAACTGCCTGCACGTATGGTCCCCTTATCGATGGCCTCTCTAAGTCAGGAAGACTATATGAAGCAAAACAACTCTTTGAAGGAATGTTGGACTACGGTTGCAGGCCAAACTGTGCTATCTACAACATTCTCATTAACGGGTTTGGAAAAGCAGGTGAGGCTGATGCAGCGTGTGCATTGTTCAAAAGGATGGTAAAAGAAGGAGTAAGACCGGATCTCAAGACTTACTCGGTTCTAGTAGATTGCCTTTGTATGGTCGGAAGAGTTGATGAAGGCTTGCATTACTTCAAGGAGCTGAAGGAATCAGGCCTCAATCCCGATGTAGTATGTTACAATCTCATAATCAATGGACTTGGGAAATCTCATAGACTTGAAGAAGCTCTTGTGCTTTTCAACGAGATGAAGACCAGCAGAGGAATCACCCCTGATCTCTACACTTACAACTCCTTGATTCTAAACTTGGGTATCGCCGGGATGGTGGAAGAAGCTGGGAAGATTTACAATGAGATTCAAAGAGCAGGTCTTGAACCGAATGTTTTTACATTTAACGCTCTGATCCGCGGATACAGTTTGTCTGGAAAACCTGAGCACGCTTACGCTGTGTACCAGACAATGGTGACCGGTGGTTTTAGCCCCAACACAGGAACATACGAACAGCTCCCCAATAGAGCTTGA

Protein Analysis

1112

Amino Acids

124.19

Weight (kDa)

6.38

Isoelectric Point (pI)

26.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 174 - 280 3e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 176 - 235 6.4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 187 - 234 1.5e-06 PPR repeat family
PPR_2 PF13041 229 - 268 3.3e-07 PPR repeat family
PPR_3 PF13812 245 - 302 1.4e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 253 - 284 6.6e-07 PPR repeat
PPR_2 PF13041 265 - 304 2e-10 PPR repeat family
PPR_1 PF12854 288 - 320 1.6e-11 PPR repeat
PPR_3 PF13812 291 - 339 2.5e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 291 - 338 1.8e-12 PPR repeat family
PPR_2 PF13041 326 - 375 1.8e-12 PPR repeat family
PPR_long PF17177 343 - 441 1.6e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 352 - 405 3.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 357 - 390 6e-11 PPR repeat
PPR_2 PF13041 361 - 409 8.2e-15 PPR repeat family
PPR_1 PF12854 393 - 425 2.5e-07 PPR repeat
PPR_2 PF13041 396 - 441 3.3e-12 PPR repeat family
PPR_3 PF13812 420 - 473 1.2e-08 Pentatricopeptide repeat domain
PPR_3 PF13812 456 - 514 5.3e-08 Pentatricopeptide repeat domain
TPR_24 PF23276 477 - 596 1.4e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 492 - 548 1.5e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 498 - 529 3e-09 PPR repeat
PPR_2 PF13041 501 - 538 2.2e-12 PPR repeat family
PPR PF01535 504 - 534 2e-08 PPR repeat
PPR_3 PF13812 524 - 586 5.7e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 532 - 565 1.5e-06 PPR repeat
PPR_2 PF13041 536 - 583 2.4e-13 PPR repeat family
TPR_24 PF23276 567 - 668 5.7e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 569 - 600 2.8e-10 PPR repeat
PPR_2 PF13041 571 - 620 2.9e-19 PPR repeat family
PPR_3 PF13812 573 - 617 2.5e-07 Pentatricopeptide repeat domain
PPR PF01535 574 - 604 7.1e-09 PPR repeat
PPR_1 PF12854 602 - 634 5.5e-07 PPR repeat
PPR_2 PF13041 606 - 653 8.4e-13 PPR repeat family
PPR_1 PF12854 637 - 670 3.1e-11 PPR repeat
PPR_2 PF13041 641 - 688 6.4e-11 PPR repeat family
PPR PF01535 644 - 671 2.6e-06 PPR repeat
PPR_3 PF13812 777 - 832 1.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 786 - 830 3.3e-08 PPR repeat family
PPR_1 PF12854 815 - 847 8.9e-07 PPR repeat
PPR_2 PF13041 818 - 867 7.6e-14 PPR repeat family
PPR PF01535 822 - 849 1e-05 PPR repeat
PPR_2 PF13041 854 - 903 2.3e-08 PPR repeat family
PPR_1 PF12854 886 - 917 1e-05 PPR repeat
PPR_long PF17177 907 - 994 1.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 913 - 969 1.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 921 - 953 3e-09 PPR repeat
PPR_2 PF13041 924 - 972 1.2e-14 PPR repeat family
PPR PF01535 928 - 957 4.7e-07 PPR repeat
TPR_24 PF23276 929 - 1021 1.9e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 948 - 1008 5.8e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 970 - 1008 3.5e-06 PPR repeat family
PPR_3 PF13812 984 - 1039 4.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 991 - 1023 8.7e-10 PPR repeat
PPR_2 PF13041 994 - 1039 7.6e-15 PPR repeat family
PPR PF01535 997 - 1024 4.6e-06 PPR repeat
PPR_2 PF13041 1035 - 1077 2e-09 PPR repeat family
PPR_3 PF13812 1053 - 1106 6.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 1065 - 1106 3.6e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014408)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31850
fragaria_vesca FvH4_2g41180 FvH4_2g41180
malus_domestica MD08G1055800.v1.1
prunus_persica Prupe.1G394600_v2.0.a1 Prupe.1G394600_v2.0.a1
pyrus_communis pycom08g04580
rosa_chinensis RchiOBHm_Chr6g0299351
rosa_laevigata RLG00000011440
rosa_multiflora Rmu_ssc0000374.1_g000003
rosa_roxburghii Rroxscaffold_7G00168770
rosa_rugosa Rorug06G0287500
rosa_samantha Rh6AG399000 Rh6BG407400 Rh6CG413300 Rh6DG400200
rosa_wichuraiana Rw6G034930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1518, 2364
AarI CACCTGC 1 cut(s) 2807
Acc36I ACCTGC 3 cut(s) 1733, 2807, 3173
AccB1I GGYRCC 1 cut(s) 2257
AccB7I CCANNNNNTGG 2 cut(s) 1240, 3277
AccI GTMKAC 1 cut(s) 2540
AccII CGCG 3 cut(s) 8, 90, 3225
AciI CCGC 6 cut(s) 847, 1218, 1442, 2379, 3223, 3225
AclWI GGATC 2 cut(s) 2886, 3214
AcsI RAATTY 6 cut(s) 331, 337, 542, 1132, 1764, 2321
AcuI CTGAAG 4 cut(s) 863, 1727, 2196, 2981
AfaI GTAC 4 cut(s) 913, 1080, 1307, 3269
AfiI CCNNNNNNNGG 5 cut(s) 1240, 2458, 2917, 3277, 3305
AflII CTTAAG 1 cut(s) 596
AflIII ACRYGT 1 cut(s) 2418
AgeI ACCGGT 2 cut(s) 1236, 3284
AjiI CACGTC 1 cut(s) 2419
AjnI CCWGG 2 cut(s) 2053, 2253
Alw21I GWGCWC 5 cut(s) 233, 1357, 2305, 2441, 3255
Alw26I GTCTC 8 cut(s) 144, 190, 350, 743, 1281, 1355, 1425, 2299
AlwI GGATC 2 cut(s) 2886, 3214
AlwNI CAGNNNCTG 1 cut(s) 326
AoxI GGCC 5 cut(s) 1773, 2067, 2698, 2768, 2971
ApeKI GCWGC 2 cut(s) 1573, 2831
ApoI RAATTY 6 cut(s) 331, 337, 542, 1132, 1764, 2321
ArsI GACNNNNNNTTYG 4 cut(s) 2501, 2533, 3169, 3201
AseI ATTAAT 1 cut(s) 822
AsiGI ACCGGT 2 cut(s) 1236, 3284
AspS9I GGNCC 5 cut(s) 869, 1171, 1450, 1498, 2684
AsuC2I CCSGG 2 cut(s) 2590, 3136
AsuII TTCGAA 1 cut(s) 948
AvaII GGWCC 5 cut(s) 869, 1171, 1450, 1498, 2684
BaeI ACNNNNGTAYC 4 cut(s) 1162, 1195, 3112, 3145
BanI GGYRCC 1 cut(s) 2257
BanII GRGCYC 1 cut(s) 233
BauI CACGAG 1 cut(s) 1106
BbsI GAAGAC 5 cut(s) 973, 1214, 2157, 2722, 3074
Bbv12I GWGCWC 5 cut(s) 233, 1357, 2305, 2441, 3255
BbvI GCAGC 2 cut(s) 1560, 2843
BceAI ACGGC 1 cut(s) 2180
BciT130I CCWGG 2 cut(s) 2055, 2255
BciVI GTATCC 2 cut(s) 448, 3221
BclI TGATCA 1 cut(s) 1632
BcnI CCSGG 2 cut(s) 2590, 3136
BcoDI GTCTC 8 cut(s) 144, 190, 350, 743, 1281, 1355, 1425, 2299
BfaI CTAG 7 cut(s) 603, 663, 918, 1433, 1748, 1877, 2900
BfmI CTRYAG 4 cut(s) 1426, 1537, 1713, 2426
BfrI CTTAAG 1 cut(s) 596
BfuAI ACCTGC 3 cut(s) 1733, 2807, 3173
BfuI GTATCC 2 cut(s) 448, 3221
BglII AGATCT 2 cut(s) 1027, 2332
BisI GCNGC 2 cut(s) 1574, 2832
BlsI GCNGC 2 cut(s) 1575, 2833
Bme1390I CCNGG 4 cut(s) 2055, 2255, 2590, 3136
Bme18I GGWCC 5 cut(s) 869, 1171, 1450, 1498, 2684
BmgBI CACGTC 1 cut(s) 2419
BmgT120I GGNCC 5 cut(s) 869, 1171, 1450, 1498, 2684
BmiI GGNNCC 5 cut(s) 1172, 1451, 1500, 2259, 2686
BmrFI CCNGG 4 cut(s) 2055, 2255, 2590, 3136
BpiI GAAGAC 5 cut(s) 973, 1214, 2157, 2722, 3074
BpmI CTGGAG 3 cut(s) 126, 175, 1359
Bpu10I CCTNAGC 1 cut(s) 3249
Bpu14I TTCGAA 1 cut(s) 948
BpuEI CTTGAG 3 cut(s) 422, 1379, 2867
BpuMI CCSGG 2 cut(s) 2590, 3136
Bsa29I ATCGAT 1 cut(s) 2694
BsaAI YACGTR 2 cut(s) 1305, 2679
BsaI GGTCTC 1 cut(s) 144
BsaJI CCNNGG 3 cut(s) 1174, 2053, 3223
BsaWI WCCGGW 3 cut(s) 1236, 2875, 3284
BsaXI ACNNNNNCTCC 4 cut(s) 1332, 1362, 2337, 2367
Bsc4I CCNNNNNNNGG 5 cut(s) 1240, 2458, 2917, 3277, 3305
Bse118I RCCGGY 3 cut(s) 1236, 2131, 3284
Bse1I ACTGG 6 cut(s) 109, 158, 1500, 2019, 2070, 2446
Bse3DI GCAATG 3 cut(s) 627, 1733, 1992
BseBI CCWGG 2 cut(s) 2055, 2255
BseCI ATCGAT 1 cut(s) 2694
BseDI CCNNGG 3 cut(s) 1174, 2053, 3223
BseLI CCNNNNNNNGG 5 cut(s) 1240, 2458, 2917, 3277, 3305
BseMI GCAATG 3 cut(s) 627, 1733, 1992
BseMII CTCAG 5 cut(s) 345, 1572, 2199, 2310, 3240
BseNI ACTGG 6 cut(s) 109, 158, 1500, 2019, 2070, 2446
BseXI GCAGC 2 cut(s) 1560, 2843
BseYI CCCAGC 3 cut(s) 326, 1127, 3152
BsgI GTGCAG 4 cut(s) 1820, 2026, 2142, 2659
Bsh1236I CGCG 3 cut(s) 8, 90, 3225
BshFI GGCC 5 cut(s) 1775, 2069, 2700, 2770, 2973
BshNI GGYRCC 1 cut(s) 2257
BshTI ACCGGT 2 cut(s) 1236, 3284
BshVI ATCGAT 1 cut(s) 2694
BsiHKAI GWGCWC 5 cut(s) 233, 1357, 2305, 2441, 3255
BsiSI CCGG 6 cut(s) 1237, 2132, 2589, 2876, 3135, 3285
BslFI GGGAC 6 cut(s) 1184, 1463, 1484, 1904, 2669, 2670
BslI CCNNNNNNNGG 5 cut(s) 1240, 2458, 2917, 3277, 3305
BsmAI GTCTC 8 cut(s) 144, 190, 350, 743, 1281, 1355, 1425, 2299
BsmBI CGTCTC 2 cut(s) 1425, 2299
BsmFI GGGAC 6 cut(s) 1184, 1463, 1484, 1904, 2669, 2670
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 5 cut(s) 1775, 2069, 2700, 2770, 2973
Bso31I GGTCTC 1 cut(s) 144
Bsp119I TTCGAA 1 cut(s) 948
Bsp1286I GDGCHC 5 cut(s) 233, 1357, 2305, 2441, 3255
Bsp143I GATC 9 cut(s) 642, 979, 1027, 1632, 2332, 2650, 2878, 3091, 3219
Bsp68I TCGCGA 1 cut(s) 90
BspACI CCGC 6 cut(s) 847, 1218, 1442, 2379, 3223, 3225
BspANI GGCC 5 cut(s) 1775, 2069, 2700, 2770, 2973
BspCNI CTCAG 5 cut(s) 346, 1573, 2200, 2309, 3241
BspDI ATCGAT 1 cut(s) 2694
BspFNI CGCG 3 cut(s) 8, 90, 3225
BspLI GGNNCC 5 cut(s) 1172, 1451, 1500, 2259, 2686
BspMI ACCTGC 3 cut(s) 1733, 2807, 3173
BspPI GGATC 2 cut(s) 2886, 3214
BspQI GCTCTTC 1 cut(s) 2429
BspT104I TTCGAA 1 cut(s) 948
BspT107I GGYRCC 1 cut(s) 2257
BspTI CTTAAG 1 cut(s) 596
BspTNI GGTCTC 1 cut(s) 144
BsrDI GCAATG 3 cut(s) 627, 1733, 1992
BsrFI RCCGGY 3 cut(s) 1236, 2131, 3284
BsrI ACTGG 6 cut(s) 109, 158, 1500, 2019, 2070, 2446
BssAI RCCGGY 3 cut(s) 1236, 2131, 3284
BssECI CCNNGG 3 cut(s) 1174, 2053, 3223
BssMI GATC 9 cut(s) 642, 979, 1027, 1632, 2332, 2650, 2878, 3091, 3219
BssSI CACGAG 1 cut(s) 1106
BssT1I CCWWGG 1 cut(s) 1174
Bst2BI CACGAG 1 cut(s) 1106
Bst2UI CCWGG 2 cut(s) 2055, 2255
Bst4CI ACNGT 9 cut(s) 892, 1260, 1430, 1541, 1717, 2517, 2762, 2777, 3233
Bst6I CTCTTC 5 cut(s) 158, 685, 2052, 2429, 2920
BstAFI CTTAAG 1 cut(s) 596
BstAPI GCANNNNNTGC 3 cut(s) 521, 2445, 2837
BstBAI YACGTR 2 cut(s) 1305, 2679
BstBI TTCGAA 1 cut(s) 948
BstC8I GCNNGC 7 cut(s) 513, 1740, 2381, 2674, 2768, 2942, 3255
BstDEI CTNAG 7 cut(s) 354, 1226, 1581, 2208, 2296, 2706, 3249
BstDSI CCRYGG 1 cut(s) 3223
BstEII GGTNACC 1 cut(s) 3280
BstFNI CGCG 3 cut(s) 8, 90, 3225
BstKTI GATC 9 cut(s) 645, 982, 1030, 1635, 2335, 2653, 2881, 3094, 3222
BstMAI GTCTC 8 cut(s) 144, 190, 350, 743, 1281, 1355, 1425, 2299
BstMBI GATC 9 cut(s) 642, 979, 1027, 1632, 2332, 2650, 2878, 3091, 3219
BstMWI GCNNNNNNNGC 6 cut(s) 521, 1415, 2445, 2822, 2831, 2837
BstNI CCWGG 2 cut(s) 2055, 2255
BstNSI RCATGY 1 cut(s) 373
BstPI GGTNACC 1 cut(s) 3280
BstSCI CCNGG 4 cut(s) 2053, 2253, 2588, 3134
BstSFI CTRYAG 4 cut(s) 1426, 1537, 1713, 2426
BstSNI TACGTA 1 cut(s) 1305
BstUI CGCG 3 cut(s) 8, 90, 3225
BstV1I GCAGC 2 cut(s) 1560, 2843
BstV2I GAAGAC 5 cut(s) 973, 1214, 2157, 2722, 3074
BstX2I RGATCY 3 cut(s) 1027, 2332, 2878
BstXI CCANNNNNNTGG 1 cut(s) 1108
BstYI RGATCY 3 cut(s) 1027, 2332, 2878
Bsu15I ATCGAT 1 cut(s) 2694
BsuI GTATCC 2 cut(s) 448, 3221
BsuRI GGCC 5 cut(s) 1775, 2069, 2700, 2770, 2973
BsuTUI ATCGAT 1 cut(s) 2694
BtgI CCRYGG 1 cut(s) 3223
BtrI CACGTC 1 cut(s) 2419
BtsI GCAGTG 1 cut(s) 1293
BtsIMutI CAGTG 7 cut(s) 151, 327, 888, 1044, 1293, 1357, 2439
BtuMI TCGCGA 1 cut(s) 90
BveI ACCTGC 3 cut(s) 1733, 2807, 3173
Cac8I GCNNGC 7 cut(s) 513, 1740, 2381, 2674, 2768, 2942, 3255
CaiI CAGNNNCTG 1 cut(s) 326
Cfr10I RCCGGY 3 cut(s) 1236, 2131, 3284
Cfr13I GGNCC 5 cut(s) 869, 1171, 1450, 1498, 2684
Cfr42I CCGCGG 1 cut(s) 3226
ClaI ATCGAT 1 cut(s) 2694
CseI GACGC 1 cut(s) 1407
Csp6I GTAC 4 cut(s) 912, 1079, 1306, 3268
CspAI ACCGGT 2 cut(s) 1236, 3284
CviAII CATG 6 cut(s) 190, 370, 1076, 1522, 1909, 2546
CviQI GTAC 4 cut(s) 912, 1079, 1306, 3268
DdeI CTNAG 7 cut(s) 354, 1226, 1581, 2208, 2296, 2706, 3249
DpnI GATC 9 cut(s) 644, 981, 1029, 1634, 2334, 2652, 2880, 3093, 3221
DpnII GATC 9 cut(s) 642, 979, 1027, 1632, 2332, 2650, 2878, 3091, 3219
DraI TTTAAA 1 cut(s) 276
Eam1104I CTCTTC 5 cut(s) 158, 685, 2052, 2429, 2920
EarI CTCTTC 5 cut(s) 158, 685, 2052, 2429, 2920
Ecl136II GAGCTC 1 cut(s) 231
Eco105I TACGTA 1 cut(s) 1305
Eco130I CCWWGG 1 cut(s) 1174
Eco147I AGGCCT 1 cut(s) 2973
Eco24I GRGCYC 1 cut(s) 233
Eco31I GGTCTC 1 cut(s) 144
Eco47I GGWCC 5 cut(s) 869, 1171, 1450, 1498, 2684
Eco53kI GAGCTC 1 cut(s) 231
Eco57I CTGAAG 4 cut(s) 863, 1727, 2196, 2981
Eco91I GGTNACC 1 cut(s) 3280
EcoICRI GAGCTC 1 cut(s) 231
EcoO65I GGTNACC 1 cut(s) 3280
EcoRII CCWGG 2 cut(s) 2053, 2253
EcoT14I CCWWGG 1 cut(s) 1174
EcoT22I ATGCAT 1 cut(s) 565
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 1174
Esp3I CGTCTC 2 cut(s) 1425, 2299
FaeI CATG 6 cut(s) 193, 373, 1079, 1525, 1912, 2549
FalI AAGNNNNNCTT 2 cut(s) 3030, 3062
FaqI GGGAC 6 cut(s) 1184, 1463, 1484, 1904, 2669, 2670
FatI CATG 6 cut(s) 189, 369, 1075, 1521, 1908, 2545
FauI CCCGC 2 cut(s) 1435, 2372
FauNDI CATATG 1 cut(s) 832
FbaI TGATCA 1 cut(s) 1632
FblI GTMKAC 1 cut(s) 2540
Fnu4HI GCNGC 2 cut(s) 1574, 2832
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 2 cut(s) 1574, 2832
FspBI CTAG 7 cut(s) 603, 663, 918, 1433, 1748, 1877, 2900
GluI GCNGC 2 cut(s) 1574, 2832
GsaI CCCAGC 3 cut(s) 330, 1131, 3156
GsuI CTGGAG 3 cut(s) 126, 175, 1359
HaeIII GGCC 5 cut(s) 1775, 2069, 2700, 2770, 2973
HapII CCGG 6 cut(s) 1237, 2132, 2589, 2876, 3135, 3285
HgaI GACGC 1 cut(s) 1407
Hin1II CATG 6 cut(s) 193, 373, 1079, 1525, 1912, 2549
HindIII AAGCTT 1 cut(s) 378
HpaII CCGG 6 cut(s) 1237, 2132, 2589, 2876, 3135, 3285
Hpy188I TCNGA 9 cut(s) 144, 202, 531, 1398, 1582, 2209, 2270, 2924, 3219
Hpy99I CGWCG 1 cut(s) 2513
HpyCH4III ACNGT 9 cut(s) 892, 1260, 1430, 1541, 1717, 2517, 2762, 2777, 3233
HpyCH4IV ACGT 4 cut(s) 1304, 2292, 2418, 2678
HpyF10VI GCNNNNNNNGC 6 cut(s) 521, 1415, 2445, 2822, 2831, 2837
HpyF3I CTNAG 7 cut(s) 354, 1226, 1581, 2208, 2296, 2706, 3249
HpySE526I ACGT 4 cut(s) 1304, 2292, 2418, 2678
Hsp92II CATG 6 cut(s) 193, 373, 1079, 1525, 1912, 2549
Ksp22I TGATCA 1 cut(s) 1632
KspI CCGCGG 1 cut(s) 3226
Kzo9I GATC 9 cut(s) 642, 979, 1027, 1632, 2332, 2650, 2878, 3091, 3219
LguI GCTCTTC 1 cut(s) 2429
LmnI GCTCC 3 cut(s) 1399, 2956, 3327
Lsp1109I GCAGC 2 cut(s) 1560, 2843
MaeI CTAG 7 cut(s) 603, 663, 918, 1433, 1748, 1877, 2900
MaeII ACGT 4 cut(s) 1304, 2292, 2418, 2678
MalI GATC 9 cut(s) 644, 981, 1029, 1634, 2334, 2652, 2880, 3093, 3221
MboI GATC 9 cut(s) 642, 979, 1027, 1632, 2332, 2650, 2878, 3091, 3219
MfeI CAATTG 1 cut(s) 2116
MflI RGATCY 3 cut(s) 1027, 2332, 2878
MhlI GDGCHC 5 cut(s) 233, 1357, 2305, 2441, 3255
MlyI GAGTC 5 cut(s) 493, 1239, 1283, 1867, 2240
MmeI TCCRAC 4 cut(s) 1221, 2368, 2733, 2902
Mph1103I ATGCAT 1 cut(s) 565
MslI CAYNNNNRTG 2 cut(s) 1106, 1526
MspA1I CMGCKG 1 cut(s) 3225
MspCI CTTAAG 1 cut(s) 596
MspI CCGG 6 cut(s) 1237, 2132, 2589, 2876, 3135, 3285
MspR9I CCNGG 4 cut(s) 2055, 2255, 2590, 3136
MunI CAATTG 1 cut(s) 2116
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 2 cut(s) 2055, 2255
MvnI CGCG 3 cut(s) 8, 90, 3225
MwoI GCNNNNNNNGC 6 cut(s) 521, 1415, 2445, 2822, 2831, 2837
NciI CCSGG 2 cut(s) 2590, 3136
NdeI CATATG 1 cut(s) 832
NdeII GATC 9 cut(s) 642, 979, 1027, 1632, 2332, 2650, 2878, 3091, 3219
NlaIII CATG 6 cut(s) 193, 373, 1079, 1525, 1912, 2549
NlaIV GGNNCC 5 cut(s) 1172, 1451, 1500, 2259, 2686
NmuCI GTSAC 4 cut(s) 1720, 1870, 2243, 3280
NruI TCGCGA 1 cut(s) 90
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 1 cut(s) 373
NspV TTCGAA 1 cut(s) 948
PaqCI CACCTGC 1 cut(s) 2807
PceI AGGCCT 1 cut(s) 2973
PciSI GCTCTTC 1 cut(s) 2429
PctI GAATGC 1 cut(s) 565
PflMI CCANNNNNTGG 2 cut(s) 1240, 3277
PinAI ACCGGT 2 cut(s) 1236, 3284
PkrI GCNGC 2 cut(s) 1575, 2833
PleI GAGTC 5 cut(s) 492, 1238, 1282, 1867, 2240
PpsI GAGTC 5 cut(s) 492, 1238, 1282, 1867, 2240
Ppu21I YACGTR 2 cut(s) 1305, 2679
PshBI ATTAAT 1 cut(s) 822
PsiI TTATAA 2 cut(s) 1518, 2364
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 2 cut(s) 2053, 2253
PspEI GGTNACC 1 cut(s) 3280
PspFI CCCAGC 3 cut(s) 326, 1127, 3152
PspGI CCWGG 2 cut(s) 2053, 2253
PspN4I GGNNCC 5 cut(s) 1172, 1451, 1500, 2259, 2686
PspPI GGNCC 5 cut(s) 869, 1171, 1450, 1498, 2684
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 3 cut(s) 1027, 2332, 2878
RruI TCGCGA 1 cut(s) 90
RsaI GTAC 4 cut(s) 913, 1080, 1307, 3269
RsaNI GTAC 4 cut(s) 912, 1079, 1306, 3268
RseI CAYNNNNRTG 2 cut(s) 1106, 1526
SacI GAGCTC 1 cut(s) 233
SacII CCGCGG 1 cut(s) 3226
SapI GCTCTTC 1 cut(s) 2429
SatI GCNGC 2 cut(s) 1574, 2832
Sau3AI GATC 9 cut(s) 642, 979, 1027, 1632, 2332, 2650, 2878, 3091, 3219
Sau96I GGNCC 5 cut(s) 869, 1171, 1450, 1498, 2684
SchI GAGTC 5 cut(s) 493, 1239, 1283, 1867, 2240
ScrFI CCNGG 4 cut(s) 2055, 2255, 2590, 3136
SduI GDGCHC 5 cut(s) 233, 1357, 2305, 2441, 3255
SfcI CTRYAG 4 cut(s) 1426, 1537, 1713, 2426
Sfr303I CCGCGG 1 cut(s) 3226
SfuI TTCGAA 1 cut(s) 948
SgrBI CCGCGG 1 cut(s) 3226
SinI GGWCC 5 cut(s) 869, 1171, 1450, 1498, 2684
SmiMI CAYNNNNRTG 2 cut(s) 1106, 1526
SmlI CTYRAG 4 cut(s) 401, 596, 1358, 2882
SmoI CTYRAG 4 cut(s) 401, 596, 1358, 2882
SnaBI TACGTA 1 cut(s) 1305
SseBI AGGCCT 1 cut(s) 2973
SsiI CCGC 6 cut(s) 847, 1218, 1442, 2379, 3223, 3225
SspMI CTAG 7 cut(s) 603, 663, 918, 1433, 1748, 1877, 2900
SstI GAGCTC 1 cut(s) 233
StuI AGGCCT 1 cut(s) 2973
StyD4I CCNGG 4 cut(s) 2053, 2253, 2588, 3134
StyI CCWWGG 1 cut(s) 1174
TaaI ACNGT 9 cut(s) 892, 1260, 1430, 1541, 1717, 2517, 2762, 2777, 3233
TaiI ACGT 4 cut(s) 1307, 2295, 2421, 2681
TaqII GACCGA 1 cut(s) 1467
TatI WGTACW 1 cut(s) 911
TscAI CASTG 7 cut(s) 158, 327, 895, 1044, 1300, 1357, 2446
TseFI GTSAC 4 cut(s) 1720, 1870, 2243, 3280
TseI GCWGC 2 cut(s) 1573, 2831
Tsp45I GTSAC 4 cut(s) 1720, 1870, 2243, 3280
TspGWI ACGGA 1 cut(s) 1336
TspRI CASTG 7 cut(s) 158, 327, 895, 1044, 1300, 1357, 2446
Van91I CCANNNNNTGG 2 cut(s) 1240, 3277
Vha464I CTTAAG 1 cut(s) 596
VpaK11BI GGWCC 5 cut(s) 869, 1171, 1450, 1498, 2684
VspI ATTAAT 1 cut(s) 822
XapI RAATTY 6 cut(s) 331, 337, 542, 1132, 1764, 2321
XceI RCATGY 1 cut(s) 373
XmiI GTMKAC 1 cut(s) 2540
XspI CTAG 7 cut(s) 603, 663, 918, 1433, 1748, 1877, 2900
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.