Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0014408

Physical Location & Seq

Genomic Coordinates Reverse (-)

58944834 .. 58947104

Gene Structure 2271 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG400200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGAAAGCGGATGGTTATGCTCCTGATGTGGTTACTTTCACTATACTGGTTGATTCTTTATGCAAAGCTGGAAATGTAGATGAGGCATTCAATATGTTGGATATCATGAGGAAAGAAGGGGTCTCACCAAATCTTCATACCTACAACACGTTAATTTGTGGACTTCTGCGGTTATGTAGGTTGGATGAGGCATTGGAACTCTTCAACAGTATGGATTCTCTTGGTGTTGCACCTACTGCTTACACATACATCCTTTTCATTGACTATTATGGAAAGTCTGGTGACTCGGGAAAAGCTATTGAGGCATTTGAGAGGATGAAAACTCGAGGCATTGTTCCTAATATTGTTGCTTGTAATGCCTCTTTGTATGGTCTTGCTGAGGAGGGTAGACTTCAAGATGCAAAACATATTTATGATGAGCTTATATACAGTGGGCTTTCTCCGGATTCAGTAACTTATAACATGATGATGAAGTGCTATAGTAAGGTGGGGCAAATAGATGAAGCCATCAAGTTACTCTCAGAGATGGAAAGAAATGGATGTGAGGCTGATGTCATCATAGTTAATTCGTTGATTGACATGCTTTACAAGGCTGGCCGAGTAGATGAGGCATGGCAAATGTTTTACAGAATGAAGGAAATGAAGCTCACTCCAACAGTTGTGACCTACAATACATTACTGGCTGCATTAGGGAAAGAGGGTCAAGTCAGAAAAGCTATTGAAATGTTTGAGAATATGACTGAACAAGGGTGTCCTCCAAATGCAATAACTTTCAACACACTCCTGAATTGCCTTTGCAAGAATGATGAGGTCAATTTGGCCTTGAAAATGCTTTGCAAAATGACCGTGATGAATTGCTGTCCTGATGTTTTGACCTATAACACTATAATTTATGGCTTAATCAGAGAAAACAGAATTGATTATGCATTCTGGTTCTTCCATCAGATGAAGAAACGCCTATTTCCTGATCATATAACATTGTGCACCCTCCTTCCGGGTGTAGTAAAGGATGGGCGCATTGAGGATGCTCTCAAAGTTGCTGAGGACTTCGCATATCAGGTTGGGGTTCAAGCAGACAGGCCTTTTTGGGAAGATTTGATTGGAGGGGTTGTGATTCAAGCTGAAGTAGACCGAGCCATTTTATTTGCTGAAAGGTTGATTTCTGAGAGGATTTGCCTAGATGATTCTGTTCTGATACCTCTACTTCGATTTTTGTGTACACGTAGGAAAACTCTTGATGCTCAGAATTTGTTTGCAAAGTTTACTGGAACTCTTGGAGTTAAGCCAACACTAGAAGCATATAACTGTTTGATCGAATGGCTTCTTAAAGACCATTTCACTGAACAAGCTTGGGATCTATTCAAGGAGATGAAAATTGCTGGCTGTGCCCCTGATGTATTCACCTACAATTTGTTACTTGATGCTCATGGGAAGTCTGGGAACATCACTGAACTCTTTGAATTGTATGATGAGATGATTTGCAGGGGATACAAGCCTAACACCATAACGCACAATATAGTTATCTCCAGTCTTGTGAAATCTGATAGCCTAGACAGGGCTATAGATCTATATTATGATCTTGTAAGTGGTGATTTTTCTCCTTCTCCTTGTACATACGGCCCTCTCATAGACGGACTTTTCAAGTCGGGAAGACTAGAGGAAGCAATGCATTTCTTTGAGGAGATGGCAGAATATGGATGTAAGCCTAATTGTGCGATCTTCAATATTCTTATAAATGGTTTTTCGAAAACAGGTGATGTGGAAACTGCTTGCGAGTTGTTTAAAAGGATGATTAAAGAAGGAATAAGACCAGACCTGAAGTCGTACACCATTCTTGTGGATTGCTACTGCCAGGCTGGAAGAGTGGATGATGCTTTGCACTATTTTGAGGAGTTAAGACGAAGTGGCCTTGATCCTGATTCAGTTTCCTACAACCTTATGATTAATGGACTTGGAAGATCACGGAGGATGGAGGAAGCTCTCGCTCTTTATGATGAAATGCGGACTAGAAGAATTACTCCTGATATTTTCACCTACAATTCATTAATACTCAATCTTGGACTTGTTGGAATGGTGGAGCAAGCAGGAAGGATTTATAAAGAGCTACAACTTACTGGTCTTGAACCTGATGTGTTTACTTACAATGCTCTCATTCGGCTGCATAGCACTTCTGGAAATCCAGATGATGCTTATGCAGTCTACAAGAACATGATGGTTGGCGGCTGCAGTCCCAATGTAGGAACATATGCACAGCTTCCCAATCAAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

756

Amino Acids

85.37

Weight (kDa)

4.98

Isoelectric Point (pI)

35.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	1 - 52	4e-10	Pentatricopeptide repeat domain
PPR_long	PF17177	1 - 90	1e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	5 - 37	9.9e-14	PPR repeat
PPR_2	PF13041	8 - 56	5.7e-17	PPR repeat family
PPR	PF01535	11 - 41	3.3e-07	PPR repeat
PPR_1	PF12854	40 - 71	1.1e-07	PPR repeat
PPR_2	PF13041	46 - 87	5.5e-11	PPR repeat family
PPR	PF01535	47 - 76	2.6e-06	PPR repeat
PPR_3	PF13812	67 - 120	1e-05	Pentatricopeptide repeat domain
PPR_3	PF13812	104 - 161	7.5e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	113 - 158	2.7e-09	PPR repeat family
PPR_1	PF12854	145 - 177	8.1e-10	PPR repeat
PPR_2	PF13041	148 - 185	4.8e-12	PPR repeat family
TPR_24	PF23276	150 - 245	5e-08	Fungal tetratrico peptide repeats
PPR	PF01535	151 - 181	1.9e-09	PPR repeat
PPR_3	PF13812	171 - 232	2e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	179 - 212	1e-06	PPR repeat
PPR_2	PF13041	184 - 230	2.4e-12	PPR repeat family
PPR_1	PF12854	216 - 247	8.3e-09	PPR repeat
PPR_2	PF13041	218 - 267	5e-19	PPR repeat family
PPR	PF01535	221 - 251	1.1e-07	PPR repeat
PPR_3	PF13812	242 - 297	1.5e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	250 - 282	3.4e-10	PPR repeat
PPR_2	PF13041	253 - 301	2.3e-13	PPR repeat family
PPR_1	PF12854	284 - 317	1.3e-08	PPR repeat
PPR_2	PF13041	288 - 335	3.7e-09	PPR repeat family
PPR_2	PF13041	431 - 476	6e-14	PPR repeat family
PPR_3	PF13812	453 - 512	2.4e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	461 - 492	3.5e-06	PPR repeat
PPR_2	PF13041	464 - 513	5.7e-14	PPR repeat family
PPR_2	PF13041	499 - 546	6.9e-06	PPR repeat family
PPR_3	PF13812	526 - 581	1.6e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	532 - 562	9.6e-08	PPR repeat
PPR_2	PF13041	534 - 570	3.5e-08	PPR repeat family
PPR	PF01535	538 - 567	3.4e-06	PPR repeat
PPR_long	PF17177	553 - 643	3.2e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	566 - 597	3.1e-09	PPR repeat
PPR_2	PF13041	572 - 618	4.2e-14	PPR repeat family
PPR	PF01535	573 - 602	6.6e-08	PPR repeat
TPR_24	PF23276	582 - 701	4.8e-09	Fungal tetratrico peptide repeats
PPR_3	PF13812	593 - 652	3.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	601 - 631	1.1e-08	PPR repeat
PPR	PF01535	608 - 637	3.7e-07	PPR repeat
PPR_2	PF13041	608 - 652	3.2e-12	PPR repeat family
PPR_3	PF13812	629 - 683	1.1e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	636 - 668	1.4e-08	PPR repeat
PPR_2	PF13041	639 - 683	1.7e-15	PPR repeat family
PPR	PF01535	642 - 671	2.1e-08	PPR repeat
PPR_long	PF17177	644 - 750	2.9e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	674 - 719	1.7e-09	PPR repeat family
PPR_3	PF13812	697 - 750	9.5e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	709 - 750	2.3e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014408)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31850
fragaria_vesca	FvH4_2g41180 FvH4_2g41180
malus_domestica	MD08G1055800.v1.1
prunus_persica	Prupe.1G394600_v2.0.a1 Prupe.1G394600_v2.0.a1
pyrus_communis	pycom08g04580
rosa_chinensis	RchiOBHm_Chr6g0299351
rosa_laevigata	RLG00000011440
rosa_multiflora	Rmu_ssc0000374.1_g000003
rosa_roxburghii	Rroxscaffold_7G00168770
rosa_rugosa	Rorug06G0287500
rosa_samantha	Rh6AG399000 Rh6BG407400 Rh6CG413300 Rh6DG400200
rosa_wichuraiana	Rw6G034930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	459, 1733, 2097
AccI	GTMKAC	3 cut(s)	388, 1128, 2199
AccIII	TCCGGA	1 cut(s)	442
AciI	CCGC	4 cut(s)	8, 169, 2002, 2220
AclWI	GGATC	2 cut(s)	1362, 1907
AcoI	YGGCCR	1 cut(s)	595
AcsI	RAATTY	1 cut(s)	1246
AcuI	CTGAAG	2 cut(s)	1143, 1838
AfaI	GTAC	3 cut(s)	1219, 1612, 1826
AfiI	CCNNNNNNNGG	3 cut(s)	994, 1555, 2237
AflIII	ACRYGT	2 cut(s)	147, 1220
AhdI	GACNNNNNGTC	1 cut(s)	1819
AjnI	CCWGG	1 cut(s)	1851
AjuI	GAANNNNNNNTTGG	2 cut(s)	1083, 1115
Alw21I	GWGCWC	1 cut(s)	986
Alw26I	GTCTC	1 cut(s)	127
Alw44I	GTGCAC	1 cut(s)	982
AlwI	GGATC	2 cut(s)	1362, 1907
Ama87I	CYCGRG	2 cut(s)	286, 324
Aor13HI	TCCGGA	1 cut(s)	442
AoxI	GGCC	5 cut(s)	595, 819, 1079, 1618, 1906
ApaLI	GTGCAC	1 cut(s)	982
ApeKI	GCWGC	3 cut(s)	683, 2158, 2223
ApoI	RAATTY	1 cut(s)	1246
AseI	ATTAAT	2 cut(s)	1944, 2045
Asp700I	GAANNNNTTC	1 cut(s)	1320
AspLEI	GCGC	1 cut(s)	1017
AspS9I	GGNCC	1 cut(s)	1619
AsuC2I	CCSGG	1 cut(s)	996
AsuHPI	GGTGA	6 cut(s)	117, 293, 1393, 1601, 1766, 2023
AsuII	TTCGAA	1 cut(s)	1745
AvaI	CYCGRG	2 cut(s)	286, 324
BaeGI	GKGCMC	2 cut(s)	986, 1390
BbsI	GAAGAC	1 cut(s)	1657
Bbv12I	GWGCWC	1 cut(s)	986
BbvCI	CCTCAGC	2 cut(s)	378, 1041
BbvI	GCAGC	3 cut(s)	670, 2145, 2210
BccI	CCATC	8 cut(s)	5, 515, 520, 948, 1004, 1678, 1963, 2206
BceAI	ACGGC	1 cut(s)	1633
BciT130I	CCWGG	1 cut(s)	1853
BciVI	GTATCC	1 cut(s)	1481
BclI	TGATCA	1 cut(s)	967
BcnI	CCSGG	1 cut(s)	996
BcoDI	GTCTC	1 cut(s)	127
BfaI	CTAG	5 cut(s)	1178, 1292, 1550, 1655, 2007
BfmI	CTRYAG	3 cut(s)	478, 1560, 2224
BfuI	GTATCC	1 cut(s)	1481
BglII	AGATCT	1 cut(s)	1564
BisI	GCNGC	4 cut(s)	684, 2159, 2221, 2224
BlsI	GCNGC	4 cut(s)	685, 2160, 2222, 2225
Bme1390I	CCNGG	2 cut(s)	996, 1853
BmeRI	GACNNNNNGTC	1 cut(s)	1819
BmeT110I	CYCGRG	2 cut(s)	286, 324
BmgT120I	GGNCC	1 cut(s)	1619
BmrFI	CCNGG	2 cut(s)	996, 1853
BmsI	GCATC	6 cut(s)	388, 1015, 1228, 1411, 1861, 2176
BpiI	GAAGAC	1 cut(s)	1657
BpmI	CTGGAG	1 cut(s)	1510
Bpu10I	CCTNAGC	2 cut(s)	378, 1041
Bpu14I	TTCGAA	1 cut(s)	1745
BpuMI	CCSGG	1 cut(s)	996
BsaAI	YACGTR	1 cut(s)	1223
BsaI	GGTCTC	1 cut(s)	127
BsaWI	WCCGGW	1 cut(s)	442
BsaXI	ACNNNNNCTCC	2 cut(s)	1095, 1125
Bsc4I	CCNNNNNNNGG	3 cut(s)	994, 1555, 2237
Bse1I	ACTGG	5 cut(s)	51, 684, 1270, 1527, 2119
Bse3DI	GCAATG	1 cut(s)	1671
BseAI	TCCGGA	1 cut(s)	442
BseBI	CCWGG	1 cut(s)	1853
BseLI	CCNNNNNNNGG	3 cut(s)	994, 1555, 2237
BseMI	GCAATG	1 cut(s)	1671
BseMII	CTCAG	5 cut(s)	369, 534, 1032, 1155, 1256
BseNI	ACTGG	5 cut(s)	51, 684, 1270, 1527, 2119
BseRI	GAGGAG	3 cut(s)	395, 1694, 1904
BseSI	GKGCMC	2 cut(s)	986, 1390
BseXI	GCAGC	3 cut(s)	670, 2145, 2210
BshFI	GGCC	5 cut(s)	597, 821, 1081, 1620, 1908
BsiHKAI	GWGCWC	1 cut(s)	986
BsiHKCI	CYCGRG	2 cut(s)	286, 324
BsiSI	CCGG	2 cut(s)	443, 995
BslFI	GGGAC	1 cut(s)	2214
BslI	CCNNNNNNNGG	3 cut(s)	994, 1555, 2237
BsmAI	GTCTC	1 cut(s)	127
BsmFI	GGGAC	1 cut(s)	2214
BsmI	GAATGC	2 cut(s)	86, 926
BsnI	GGCC	5 cut(s)	597, 821, 1081, 1620, 1908
Bso31I	GGTCTC	1 cut(s)	127
BsoBI	CYCGRG	2 cut(s)	286, 324
Bsp119I	TTCGAA	1 cut(s)	1745
Bsp1286I	GDGCHC	2 cut(s)	986, 1390
Bsp13I	TCCGGA	1 cut(s)	442
Bsp1407I	TGTACA	2 cut(s)	1217, 1610
Bsp143I	GATC	8 cut(s)	967, 1311, 1354, 1564, 1576, 1716, 1912, 1958
BspACI	CCGC	4 cut(s)	8, 169, 2002, 2220
BspANI	GGCC	5 cut(s)	597, 821, 1081, 1620, 1908
BspCNI	CTCAG	5 cut(s)	370, 533, 1033, 1156, 1255
BspEI	TCCGGA	1 cut(s)	442
BspHI	TCATGA	1 cut(s)	105
BspMAI	CTGCAG	1 cut(s)	2228
BspPI	GGATC	2 cut(s)	1362, 1907
BspT104I	TTCGAA	1 cut(s)	1745
BspTNI	GGTCTC	1 cut(s)	127
BsrDI	GCAATG	1 cut(s)	1671
BsrGI	TGTACA	2 cut(s)	1217, 1610
BsrI	ACTGG	5 cut(s)	51, 684, 1270, 1527, 2119
BssMI	GATC	8 cut(s)	967, 1311, 1354, 1564, 1576, 1716, 1912, 1958
Bst2UI	CCWGG	1 cut(s)	1853
Bst4CI	ACNGT	5 cut(s)	209, 431, 658, 847, 1307
Bst6I	CTCTTC	2 cut(s)	206, 1855
BstAPI	GCANNNNNTGC	1 cut(s)	236
BstAUI	TGTACA	2 cut(s)	1217, 1610
BstBAI	YACGTR	1 cut(s)	1223
BstBI	TTCGAA	1 cut(s)	1745
BstC8I	GCNNGC	4 cut(s)	595, 1381, 1771, 2082
BstDEI	CTNAG	5 cut(s)	378, 520, 1041, 1164, 1242
BstHHI	GCGC	1 cut(s)	1017
BstKTI	GATC	8 cut(s)	970, 1314, 1357, 1567, 1579, 1719, 1915, 1961
BstMAI	GTCTC	1 cut(s)	127
BstMBI	GATC	8 cut(s)	967, 1311, 1354, 1564, 1576, 1716, 1912, 1958
BstMWI	GCNNNNNNNGC	4 cut(s)	236, 302, 356, 1385
BstNI	CCWGG	1 cut(s)	1853
BstNSI	RCATGY	1 cut(s)	583
BstSCI	CCNGG	2 cut(s)	994, 1851
BstSFI	CTRYAG	3 cut(s)	478, 1560, 2224
BstSLI	GKGCMC	2 cut(s)	986, 1390
BstV1I	GCAGC	3 cut(s)	670, 2145, 2210
BstV2I	GAAGAC	1 cut(s)	1657
BstX2I	RGATCY	2 cut(s)	1354, 1564
BstXI	CCANNNNNNTGG	1 cut(s)	1837
BstYI	RGATCY	2 cut(s)	1354, 1564
BsuI	GTATCC	1 cut(s)	1481
BsuRI	GGCC	5 cut(s)	597, 821, 1081, 1620, 1908
BtsIMutI	CAGTG	3 cut(s)	436, 1338, 1446
Cac8I	GCNNGC	4 cut(s)	595, 1381, 1771, 2082
CciI	TCATGA	1 cut(s)	105
CfoI	GCGC	1 cut(s)	1017
Cfr13I	GGNCC	1 cut(s)	1619
Csp6I	GTAC	3 cut(s)	1218, 1611, 1825
CviAII	CATG	6 cut(s)	106, 463, 580, 612, 1427, 2209
CviQI	GTAC	3 cut(s)	1218, 1611, 1825
DdeI	CTNAG	5 cut(s)	378, 520, 1041, 1164, 1242
DpnI	GATC	8 cut(s)	969, 1313, 1356, 1566, 1578, 1718, 1914, 1960
DpnII	GATC	8 cut(s)	967, 1311, 1354, 1564, 1576, 1716, 1912, 1958
DraI	TTTAAA	1 cut(s)	1783
DriI	GACNNNNNGTC	1 cut(s)	1819
EaeI	YGGCCR	1 cut(s)	595
Eam1104I	CTCTTC	2 cut(s)	206, 1855
Eam1105I	GACNNNNNGTC	1 cut(s)	1819
EarI	CTCTTC	2 cut(s)	206, 1855
Eco147I	AGGCCT	1 cut(s)	1081
Eco31I	GGTCTC	1 cut(s)	127
Eco32I	GATATC	1 cut(s)	103
Eco57I	CTGAAG	2 cut(s)	1143, 1838
Eco88I	CYCGRG	2 cut(s)	286, 324
EcoRII	CCWGG	1 cut(s)	1851
EcoRV	GATATC	1 cut(s)	103
EcoT22I	ATGCAT	2 cut(s)	928, 1671
FaeI	CATG	6 cut(s)	109, 466, 583, 615, 1430, 2212
FaqI	GGGAC	1 cut(s)	2214
FatI	CATG	6 cut(s)	105, 462, 579, 611, 1426, 2208
FauNDI	CATATG	1 cut(s)	2245
FbaI	TGATCA	1 cut(s)	967
FblI	GTMKAC	3 cut(s)	388, 1128, 2199
Fnu4HI	GCNGC	4 cut(s)	684, 2159, 2221, 2224
Fsp4HI	GCNGC	4 cut(s)	684, 2159, 2221, 2224
FspBI	CTAG	5 cut(s)	1178, 1292, 1550, 1655, 2007
GlaI	GCGC	1 cut(s)	1016
GluI	GCNGC	4 cut(s)	684, 2159, 2221, 2224
GsuI	CTGGAG	1 cut(s)	1510
HaeIII	GGCC	5 cut(s)	597, 821, 1081, 1620, 1908
HapII	CCGG	2 cut(s)	443, 995
HhaI	GCGC	1 cut(s)	1017
Hin1II	CATG	6 cut(s)	109, 466, 583, 615, 1430, 2212
Hin6I	GCGC	1 cut(s)	1015
HinP1I	GCGC	1 cut(s)	1015
HindIII	AAGCTT	1 cut(s)	1347
HinfI	GANTC	7 cut(s)	53, 215, 284, 446, 1114, 1184, 1919
HpaII	CCGG	2 cut(s)	443, 995
HphI	GGTGA	6 cut(s)	117, 293, 1393, 1601, 1766, 2023
Hpy188I	TCNGA	8 cut(s)	523, 710, 905, 945, 1165, 1194, 1245, 1543
HpyAV	CCTTC	6 cut(s)	110, 628, 1001, 1611, 1793, 2082
HpyCH4III	ACNGT	5 cut(s)	209, 431, 658, 847, 1307
HpyCH4IV	ACGT	2 cut(s)	149, 1222
HpyF10VI	GCNNNNNNNGC	4 cut(s)	236, 302, 356, 1385
HpyF3I	CTNAG	5 cut(s)	378, 520, 1041, 1164, 1242
HpySE526I	ACGT	2 cut(s)	149, 1222
Hsp92II	CATG	6 cut(s)	109, 466, 583, 615, 1430, 2212
HspAI	GCGC	1 cut(s)	1015
Kpn2I	TCCGGA	1 cut(s)	442
Ksp22I	TGATCA	1 cut(s)	967
Kzo9I	GATC	8 cut(s)	967, 1311, 1354, 1564, 1576, 1716, 1912, 1958
LmnI	GCTCC	2 cut(s)	25, 2077
Lsp1109I	GCAGC	3 cut(s)	670, 2145, 2210
LweI	GCATC	6 cut(s)	388, 1015, 1228, 1411, 1861, 2176
MaeI	CTAG	5 cut(s)	1178, 1292, 1550, 1655, 2007
MaeII	ACGT	2 cut(s)	149, 1222
MaeIII	GTNAC	6 cut(s)	31, 281, 451, 513, 661, 1413
MalI	GATC	8 cut(s)	969, 1313, 1356, 1566, 1578, 1718, 1914, 1960
MboI	GATC	8 cut(s)	967, 1311, 1354, 1564, 1576, 1716, 1912, 1958
MflI	RGATCY	2 cut(s)	1354, 1564
MhlI	GDGCHC	2 cut(s)	986, 1390
MlyI	GAGTC	1 cut(s)	278
MmeI	TCCRAC	4 cut(s)	78, 162, 677, 2047
Mph1103I	ATGCAT	2 cut(s)	928, 1671
MroI	TCCGGA	1 cut(s)	442
MroXI	GAANNNNTTC	1 cut(s)	1320
MslI	CAYNNNNRTG	3 cut(s)	411, 467, 1835
MspI	CCGG	2 cut(s)	443, 995
MspR9I	CCNGG	2 cut(s)	996, 1853
Mva1269I	GAATGC	2 cut(s)	86, 926
MvaI	CCWGG	1 cut(s)	1853
MwoI	GCNNNNNNNGC	4 cut(s)	236, 302, 356, 1385
NciI	CCSGG	1 cut(s)	996
NdeI	CATATG	1 cut(s)	2245
NdeII	GATC	8 cut(s)	967, 1311, 1354, 1564, 1576, 1716, 1912, 1958
NlaIII	CATG	6 cut(s)	109, 466, 583, 615, 1430, 2212
NmeAIII	GCCGAG	1 cut(s)	623
NmuCI	GTSAC	2 cut(s)	281, 661
NsiI	ATGCAT	2 cut(s)	928, 1671
NspI	RCATGY	1 cut(s)	583
NspV	TTCGAA	1 cut(s)	1745
PaeR7I	CTCGAG	1 cut(s)	324
PagI	TCATGA	1 cut(s)	105
PceI	AGGCCT	1 cut(s)	1081
PctI	GAATGC	2 cut(s)	86, 926
PdmI	GAANNNNTTC	1 cut(s)	1320
PfeI	GAWTC	6 cut(s)	53, 215, 446, 1114, 1184, 1919
PkrI	GCNGC	4 cut(s)	685, 2160, 2222, 2225
PleI	GAGTC	1 cut(s)	278
PpsI	GAGTC	1 cut(s)	278
Ppu21I	YACGTR	1 cut(s)	1223
PshBI	ATTAAT	2 cut(s)	1944, 2045
PsiI	TTATAA	3 cut(s)	459, 1733, 2097
Psp6I	CCWGG	1 cut(s)	1851
PspGI	CCWGG	1 cut(s)	1851
PspPI	GGNCC	1 cut(s)	1619
PspXI	VCTCGAGB	1 cut(s)	324
PstI	CTGCAG	1 cut(s)	2228
PsuI	RGATCY	2 cut(s)	1354, 1564
RsaI	GTAC	3 cut(s)	1219, 1612, 1826
RsaNI	GTAC	3 cut(s)	1218, 1611, 1825
RseI	CAYNNNNRTG	3 cut(s)	411, 467, 1835
SatI	GCNGC	4 cut(s)	684, 2159, 2221, 2224
Sau3AI	GATC	8 cut(s)	967, 1311, 1354, 1564, 1576, 1716, 1912, 1958
Sau96I	GGNCC	1 cut(s)	1619
SchI	GAGTC	1 cut(s)	278
ScrFI	CCNGG	2 cut(s)	996, 1853
SduI	GDGCHC	2 cut(s)	986, 1390
SfaNI	GCATC	6 cut(s)	388, 1015, 1228, 1411, 1861, 2176
SfcI	CTRYAG	3 cut(s)	478, 1560, 2224
Sfr274I	CTCGAG	1 cut(s)	324
SfuI	TTCGAA	1 cut(s)	1745
SlaI	CTCGAG	1 cut(s)	324
SmiMI	CAYNNNNRTG	3 cut(s)	411, 467, 1835
SmlI	CTYRAG	1 cut(s)	324
SmoI	CTYRAG	1 cut(s)	324
SseBI	AGGCCT	1 cut(s)	1081
SsiI	CCGC	4 cut(s)	8, 169, 2002, 2220
SspI	AATATT	2 cut(s)	343, 1726
SspMI	CTAG	5 cut(s)	1178, 1292, 1550, 1655, 2007
StuI	AGGCCT	1 cut(s)	1081
StyD4I	CCNGG	2 cut(s)	994, 1851
TaaI	ACNGT	5 cut(s)	209, 431, 658, 847, 1307
TaiI	ACGT	2 cut(s)	152, 1225
TaqI	TCGA	4 cut(s)	325, 1207, 1314, 1745
TaqII	GACCGA	1 cut(s)	1146
TatI	WGTACW	2 cut(s)	1217, 1610
TauI	GCSGC	1 cut(s)	2223
TfiI	GAWTC	6 cut(s)	53, 215, 446, 1114, 1184, 1919
TscAI	CASTG	3 cut(s)	436, 1345, 1453
TseFI	GTSAC	2 cut(s)	281, 661
TseI	GCWGC	3 cut(s)	683, 2158, 2223
Tsp45I	GTSAC	2 cut(s)	281, 661
TspGWI	ACGGA	2 cut(s)	1647, 1978
TspRI	CASTG	3 cut(s)	436, 1345, 1453
VneI	GTGCAC	1 cut(s)	982
VspI	ATTAAT	2 cut(s)	1944, 2045
XapI	RAATTY	1 cut(s)	1246
XceI	RCATGY	1 cut(s)	583
XhoI	CTCGAG	1 cut(s)	324
XmiI	GTMKAC	3 cut(s)	388, 1128, 2199
XmnI	GAANNNNTTC	1 cut(s)	1320
XspI	CTAG	5 cut(s)	1178, 1292, 1550, 1655, 2007
Zsp2I	ATGCAT	2 cut(s)	928, 1671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.