RchiOBHm_Chr6g0299351

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0014408
Physical Location & Seq
Reverse (-)
60466098 .. 60470026
3929 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26879

Sequence Viewer

Length: 3366 bp
ATGGCATTATTGATACTCTGCTCTTCAACTATGTGCTGCTGTACTAGTCTTGCTTATACTGACACCACCACTAGTAGTAGTAGGATATTTGCTATAAGCACCCACACTCCTCCTCCTGTTCTACATCCACCAAAATCAAAGGTTTGGCCTTGTGGTTCGCTGCTCAACTTGACCAGCAAGAGGAACAAAAGGTTGGGTTTCTGTGGCTTTGTGATCAAAAGGCCGCAGCAGCAGCAGGAGGTGCTGGTGCTGGTGCAGAAGAAGAACAAGCCCAAAACTTCAGTGTCTTCTGAGGAAGTTGTGACGGTTCTAAAGTCCATTTCAGACCCGACTTCTGCCTTTTCATTCTTCAAGTCCATTGCTGAGTTGCCTAGTGTGATTCACACCACTGAAACTTGCAATTACATGCTTCAACTCTTGGGTGTTCATAGGAGGGTGGATGATATGGCTTTTGTGTTTGATTTGATGCAAAAGCATATCATTAACAGGAGTGTAGACACTTACCTCATTATATTTAAGGGTCTTAATATAAGAGGCGGGATTCGAGCAGCCCCGTATGCTCTTGGAAGGTTGCGCAAAAATGGGTTTATCTTGAATGCATATTCATACAATGGCTTGATCTATATGCTTATCCAGTCTGGCTATTGTAGGGAGGCTCTGGAGGTATACCAAAGGGTGGTGTCAGAAGGACTTAAGCCGAGCCTCAAGACTTACTCAGCACTTATGGTGGCATTGGGAAAGAGAAGGGATGTTCAAACTGTTATGGGCTTACTAAAAGAGATGGAAACTTTGGGGCTCAGGCCTAATGTTTATACTTTCACTATATGCATTAGAGTACTCGGGAGGGCTGGGAAAATTGACGAGGCATATGAAATTTTCAAGAGAATGGATGATGAGGGTTGTGGCCCCGACGTTATCACTTATACTGTTCTCATTGATGCTCTTTGTAATGCAGGTAAACTTGATAATGCCAAGAAGTTGTTTGCAAATATGAAAGCAAGTGGTCATAAGCCTGACCAAGTAACCTACATTACGCTGCTGGACAAGTTTAGTGATAGTAAAGACTTGGACACAGTAAAAGAATTCTGGAGTGAAATGAAAGCTGATGGTTATGCTCCTGATGTGGTTACTTTCACTATACTGGTTGATTCTTTATGCAAAGCTGGAAATGCAGATGAGGCATTCAATATGTTGGATATCATGAGGAAAGAAGGGGTCTCACCAAATCTTCAGACCTACAACACGTTAATTTGTGGCCTTCTGCGGCTATGTAGGTTGGATGAGGCATTGGAACTCTTCAGCAGTATGGATTCTCTTGGTGTTGCACCTACAGCTTACACATACATCCTTTTCATTGACTATTATGGAAAGTCTGGTGACTCGGGAAAAGCTATTGAGGCATTTGAGAGGATGAAAACTCGAGGCATTGTTCCTAATATTGTTGCTTGTAATGCCTCTTTGTATGGTCTTGCTGAGGAGGGCAGACTTCAAGATGCAAAACATATTTATGATGAGCTTATATACAGTGGGCTTTCTCCGGATTCAGTAACTTATAACATGATGATGAAGTGCTATAGTAAGGTGGGGCAAATAGATGAAGCCATCAAGTTACTCTCAGAGATGGAAAGAAATGGGTGTGAGGCTGATGTCATCATAGTTAATTCGTTGATTGACATGCTTTACAAGGCTGGCCGAGTAGATGAGGCATGGCAAATGTTTTACAGAATGAAGGAAATGAAACTCACTCCAACAGTTGTGACCTACAATACATTACTGGCTGCATTAGGGAAAGAGGGTCAAGTCAGAAAAGCTATTGAAATGTTTGAGAATATGACTGAACAAGGGTGTCCTCCAAATGCAATAACTTTCAACACAATCCTGAATGGCCTTTGCAAGAATGATGAGGTCAATTTGGCCTTGAAAATGCTTTGCAAGATGACCGTGATGAATTGCTGTCCTGATGTTTTGACCTATAACACTATAATTTATGGCTTAATCAGAGAAAACAGAATTGATTATGCATTCTGGTTCTTCCATCAGATGAAGAAACGCCTATTTCCTGATCATATAACATTGTGCACCCTCCTTCCGGGTGTAGTAAAGGATGGGCGCATTGAGGATGCTCTCAAAGTTGCTGAGGACTTTGCATATCAGGTTGGAGTTCAAGCAGACAGGCCTTTTTGGGAAGATTTGATTGGAGGGGTTGTGATTCAAGCTGAAGTAGACCGAGCCATTTTATTTGCTGAAAGGTTGATTTCTGAGAGGATTTGCCTAGATGATTCTGTTCTGATACCTCTACTTCGATTTTTGTGTACACGTAGGAAAACTCTTGATGCTCAGAATTTGTTTGCAAAGTTTACTGGAACTCTTGGAGTTAAGCCAACACTAGAAGCATATAACTGTTTGATCGAATGGCTTCTTAAAGACCATTTCACTGAACAAGCTTGGGATCTATTCAAGGAGATGAAAATTGCTGGCTGTGCCCCTGATGTATTCACCTACAATTTGTTACTTGATGCTCATGGGAAGTCTGGGAACATCACTGAACTCTTTGAATTGTATGACGAGATGATTTGCAGGGGATACAAGCCTAACACCATAACGCACAATATAGTTATCTCCAGTCTTGTGAAATCTGATAGCCTAGACAGGGCTATAGATCTATATTATGATCTTGTAAGTGGTGATTTTTCTCCTTCTCCTTGTACATACGGCCCTCTCATAGATGGACTTTTCAAGTCGGGAAGACTAGAAGAAGCAATGCATTTCTTTGAGGAGATGGCAGAATATGGATGTAAGCCTAATTGTGCGATCTTCAATATTCTTATAAATGGTTTTTCGAAAACAGGTGATGTGGAAACTGCTTGTGAGTTGTTTAAAAGGATGATTAAAGAAGGAATAAGACCAGACCTGAAGTCGTACACCATTCTCGTGGATTGCTACTGCCAGGCTGGAAGAGTGGATGATGCTTTGCACTATTTTGAGGAGTTAAGACGAAGTGGCCTTGATCCTGATTCAGTTTCCTACAACCTTATGATTAATGGACTTGGAAGATCACGGAGGATGGAGGAAGCTCTCGCTCTTTATGATGAAATGCGGACTAGAAGAATTACTCCCGATATTTTCACCTACAATTCATTAATACTCAACCTTGGACTTGTTGGAATGGTGGAGCAAGCAGGAAGGATTTATAAAGAGCTACAACTTACTGGTCTTGAACCTGATGTGTTTACTTACAATGCTCTCATTCGGCTGCATAGCACTTCTGGAAATCCAGATGATGCTTATGCAGTCTATAAGAACATGATGGTTGGCGGCTGCAGTCCCAATGTAGGAACATATGCACAGCTTCCCAATCAAACTTGA

Protein Analysis

1121

Amino Acids

126.13

Weight (kDa)

5.86

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 186 - 296 4.4e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 192 - 248 1.1e-06 Pentatricopeptide repeat domain
PPR_long PF17177 193 - 292 1.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 237 - 280 6.8e-08 PPR repeat family
PPR_3 PF13812 257 - 315 3.2e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 265 - 296 2.7e-07 PPR repeat
PPR PF01535 271 - 301 2.4e-06 PPR repeat
PPR_2 PF13041 277 - 317 3.5e-11 PPR repeat family
PPR_1 PF12854 300 - 332 1.5e-11 PPR repeat
PPR_3 PF13812 303 - 350 7.4e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 303 - 350 3.8e-15 PPR repeat family
PPR PF01535 306 - 335 2.1e-06 PPR repeat
PPR_3 PF13812 326 - 382 3.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 341 - 387 2.4e-10 PPR repeat family
PPR_long PF17177 354 - 471 1.3e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 364 - 417 3.6e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 370 - 402 3e-12 PPR repeat
PPR_2 PF13041 373 - 421 1.3e-15 PPR repeat family
PPR PF01535 376 - 406 1.9e-06 PPR repeat
PPR_1 PF12854 405 - 436 8.1e-07 PPR repeat
PPR_2 PF13041 412 - 452 5e-10 PPR repeat family
PPR_long PF17177 463 - 587 8.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 469 - 526 1.2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 478 - 527 6.2e-10 PPR repeat family
PPR_1 PF12854 510 - 542 1.3e-09 PPR repeat
TPR_24 PF23276 510 - 610 5.6e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 513 - 550 7.9e-12 PPR repeat family
PPR PF01535 516 - 546 3e-09 PPR repeat
PPR_long PF17177 531 - 612 6.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 536 - 597 3.3e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 544 - 577 1.6e-06 PPR repeat
PPR_2 PF13041 549 - 595 3.8e-12 PPR repeat family
PPR_1 PF12854 581 - 612 1.3e-08 PPR repeat
PPR_2 PF13041 583 - 632 4.2e-19 PPR repeat family
PPR PF01535 586 - 616 1.8e-07 PPR repeat
PPR_3 PF13812 607 - 662 4.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 615 - 647 1.4e-10 PPR repeat
PPR_2 PF13041 618 - 666 1.2e-13 PPR repeat family
PPR_1 PF12854 649 - 682 2.1e-08 PPR repeat
PPR_2 PF13041 653 - 700 6e-09 PPR repeat family
PPR_2 PF13041 796 - 841 9.6e-14 PPR repeat family
PPR_3 PF13812 818 - 877 3.9e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 826 - 857 5.5e-06 PPR repeat
PPR_2 PF13041 829 - 878 9.2e-14 PPR repeat family
PPR_3 PF13812 891 - 946 2.6e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 897 - 927 1.5e-07 PPR repeat
PPR_2 PF13041 899 - 935 5.5e-08 PPR repeat family
PPR PF01535 903 - 932 5.5e-06 PPR repeat
PPR_long PF17177 918 - 1008 6.4e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 931 - 962 4.9e-09 PPR repeat
PPR_2 PF13041 937 - 983 6.7e-14 PPR repeat family
PPR PF01535 938 - 967 1.1e-07 PPR repeat
TPR_24 PF23276 948 - 1066 1.4e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 958 - 1017 5.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 966 - 996 1.7e-08 PPR repeat
PPR PF01535 973 - 1002 5.8e-07 PPR repeat
PPR_2 PF13041 973 - 1017 6.3e-12 PPR repeat family
PPR_3 PF13812 994 - 1048 1.6e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 1001 - 1033 2.2e-08 PPR repeat
PPR_2 PF13041 1004 - 1048 2.7e-15 PPR repeat family
PPR PF01535 1007 - 1036 3.4e-08 PPR repeat
PPR_long PF17177 1012 - 1115 6.6e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 1062 - 1115 1.5e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 1074 - 1115 3.7e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014408)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31850
fragaria_vesca FvH4_2g41180 FvH4_2g41180
malus_domestica MD08G1055800.v1.1
prunus_persica Prupe.1G394600_v2.0.a1 Prupe.1G394600_v2.0.a1
pyrus_communis pycom08g04580
rosa_chinensis RchiOBHm_Chr6g0299351
rosa_laevigata RLG00000011440
rosa_multiflora Rmu_ssc0000374.1_g000003
rosa_roxburghii Rroxscaffold_7G00168770
rosa_rugosa Rorug06G0287500
rosa_samantha Rh6AG399000 Rh6BG407400 Rh6CG413300 Rh6DG400200
rosa_wichuraiana Rw6G034930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1554, 2828, 3192
Acc16I TGCGCA 1 cut(s) 575
Acc36I ACCTGC 1 cut(s) 944
AccB7I CCANNNNNTGG 1 cut(s) 676
AccI GTMKAC 3 cut(s) 495, 666, 2223
AccIII TCCGGA 1 cut(s) 1537
AciI CCGC 5 cut(s) 224, 537, 1264, 3097, 3315
AclWI GGATC 2 cut(s) 2457, 3002
AcoI YGGCCR 1 cut(s) 1690
AcsI RAATTY 3 cut(s) 873, 1082, 2341
AcuI CTGAAG 5 cut(s) 264, 1214, 1282, 2238, 2933
AfaI GTAC 5 cut(s) 43, 837, 2314, 2707, 2921
AfiI CCNNNNNNNGG 5 cut(s) 180, 676, 2089, 2650, 3332
AflII CTTAAG 1 cut(s) 692
AflIII ACRYGT 2 cut(s) 1242, 2315
AhdI GACNNNNNGTC 1 cut(s) 2914
AhlI ACTAGT 2 cut(s) 44, 71
AjnI CCWGG 1 cut(s) 2946
AjuI GAANNNNNNNTTGG 4 cut(s) 176, 208, 2178, 2210
Alw21I GWGCWC 1 cut(s) 2081
Alw26I GTCTC 1 cut(s) 1222
Alw44I GTGCAC 1 cut(s) 2077
AlwI GGATC 2 cut(s) 2457, 3002
Ama87I CYCGRG 3 cut(s) 839, 1381, 1419
Aor13HI TCCGGA 1 cut(s) 1537
ApaLI GTGCAC 1 cut(s) 2077
ApoI RAATTY 3 cut(s) 873, 1082, 2341
AseI ATTAAT 2 cut(s) 3039, 3140
Asp700I GAANNNNTTC 1 cut(s) 2415
AspLEI GCGC 2 cut(s) 576, 2112
AspS9I GGNCC 2 cut(s) 905, 2714
AsuC2I CCSGG 1 cut(s) 2091
AsuHPI GGTGA 6 cut(s) 1212, 1388, 2488, 2696, 2861, 3118
AsuII TTCGAA 1 cut(s) 2840
AvaI CYCGRG 3 cut(s) 839, 1381, 1419
BaeGI GKGCMC 2 cut(s) 2081, 2485
BanII GRGCYC 1 cut(s) 798
BauI CACGAG 1 cut(s) 2930
BbsI GAAGAC 2 cut(s) 279, 2752
Bbv12I GWGCWC 1 cut(s) 2081
BbvCI CCTCAGC 2 cut(s) 1473, 2136
BceAI ACGGC 1 cut(s) 2728
BciT130I CCWGG 1 cut(s) 2948
BciVI GTATCC 1 cut(s) 2576
BclI TGATCA 2 cut(s) 213, 2062
BcnI CCSGG 1 cut(s) 2091
BcoDI GTCTC 1 cut(s) 1222
BcuI ACTAGT 2 cut(s) 44, 71
BfaI CTAG 8 cut(s) 45, 72, 372, 2273, 2387, 2645, 2750, 3102
BfmI CTRYAG 4 cut(s) 1329, 1573, 2655, 3319
BfrI CTTAAG 1 cut(s) 692
BfuAI ACCTGC 1 cut(s) 944
BfuI GTATCC 1 cut(s) 2576
BglII AGATCT 1 cut(s) 2659
BmcAI AGTACT 1 cut(s) 837
Bme1390I CCNGG 2 cut(s) 2091, 2948
BmeRI GACNNNNNGTC 1 cut(s) 2914
BmeT110I CYCGRG 3 cut(s) 839, 1381, 1419
BmgT120I GGNCC 2 cut(s) 905, 2714
BmiI GGNNCC 1 cut(s) 907
BmrFI CCNGG 2 cut(s) 2091, 2948
BmsI GCATC 8 cut(s) 456, 928, 1483, 2110, 2323, 2506, 2956, 3271
BpiI GAAGAC 2 cut(s) 279, 2752
BpmI CTGGAG 3 cut(s) 680, 1108, 2605
Bpu10I CCTNAGC 3 cut(s) 797, 1473, 2136
Bpu14I TTCGAA 1 cut(s) 2840
BpuEI CTTGAG 1 cut(s) 689
BpuMI CCSGG 1 cut(s) 2091
BsaAI YACGTR 1 cut(s) 2318
BsaI GGTCTC 1 cut(s) 1222
BsaJI CCNNGG 1 cut(s) 3151
BsaWI WCCGGW 1 cut(s) 1537
BsaXI ACNNNNNCTCC 6 cut(s) 91, 97, 121, 127, 2190, 2220
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 676, 2089, 2650, 3332
Bse1I ACTGG 6 cut(s) 634, 1146, 1779, 2365, 2622, 3214
Bse3DI GCAATG 2 cut(s) 357, 2766
BseAI TCCGGA 1 cut(s) 1537
BseBI CCWGG 1 cut(s) 2948
BseDI CCNNGG 1 cut(s) 3151
BseLI CCNNNNNNNGG 5 cut(s) 180, 676, 2089, 2650, 3332
BseMI GCAATG 2 cut(s) 357, 2766
BseMII CTCAG 9 cut(s) 282, 354, 729, 811, 1464, 1629, 2127, 2250, 2351
BseNI ACTGG 6 cut(s) 634, 1146, 1779, 2365, 2622, 3214
BseRI GAGGAG 5 cut(s) 99, 102, 1490, 2789, 2999
BseSI GKGCMC 2 cut(s) 2081, 2485
BseYI CCCAGC 1 cut(s) 848
BsgI GTGCAG 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 2081
BsiHKCI CYCGRG 3 cut(s) 839, 1381, 1419
BsiSI CCGG 2 cut(s) 1538, 2090
BslFI GGGAC 1 cut(s) 3309
BslI CCNNNNNNNGG 5 cut(s) 180, 676, 2089, 2650, 3332
BsmAI GTCTC 1 cut(s) 1222
BsmFI GGGAC 1 cut(s) 3309
BsmI GAATGC 3 cut(s) 601, 1181, 2021
Bso31I GGTCTC 1 cut(s) 1222
BsoBI CYCGRG 3 cut(s) 839, 1381, 1419
Bsp119I TTCGAA 1 cut(s) 2840
Bsp1286I GDGCHC 3 cut(s) 798, 2081, 2485
Bsp13I TCCGGA 1 cut(s) 1537
Bsp1407I TGTACA 2 cut(s) 2312, 2705
BspACI CCGC 5 cut(s) 224, 537, 1264, 3097, 3315
BspCNI CTCAG 9 cut(s) 283, 355, 728, 810, 1465, 1628, 2128, 2251, 2350
BspEI TCCGGA 1 cut(s) 1537
BspHI TCATGA 1 cut(s) 1200
BspLI GGNNCC 1 cut(s) 907
BspMAI CTGCAG 1 cut(s) 3323
BspMI ACCTGC 1 cut(s) 944
BspPI GGATC 2 cut(s) 2457, 3002
BspQI GCTCTTC 1 cut(s) 28
BspT104I TTCGAA 1 cut(s) 2840
BspTI CTTAAG 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 1222
BsrDI GCAATG 2 cut(s) 357, 2766
BsrGI TGTACA 2 cut(s) 2312, 2705
BsrI ACTGG 6 cut(s) 634, 1146, 1779, 2365, 2622, 3214
BssECI CCNNGG 1 cut(s) 3151
BssNAI GTATAC 1 cut(s) 667
BssSI CACGAG 1 cut(s) 2930
BssT1I CCWWGG 1 cut(s) 3151
Bst1107I GTATAC 1 cut(s) 667
Bst2BI CACGAG 1 cut(s) 2930
Bst2UI CCWGG 1 cut(s) 2948
Bst4CI ACNGT 8 cut(s) 307, 760, 928, 1075, 1526, 1753, 1942, 2402
Bst6I CTCTTC 3 cut(s) 28, 1301, 2950
BstAFI CTTAAG 1 cut(s) 692
BstAPI GCANNNNNTGC 1 cut(s) 241
BstAUI TGTACA 2 cut(s) 2312, 2705
BstBAI YACGTR 1 cut(s) 2318
BstBI TTCGAA 1 cut(s) 2840
BstC8I GCNNGC 3 cut(s) 1690, 2476, 3177
BstDEI CTNAG 9 cut(s) 291, 363, 715, 797, 1473, 1615, 2136, 2259, 2337
BstHHI GCGC 2 cut(s) 576, 2112
BstMAI GTCTC 1 cut(s) 1222
BstNI CCWGG 1 cut(s) 2948
BstNSI RCATGY 2 cut(s) 409, 1678
BstSCI CCNGG 2 cut(s) 2089, 2946
BstSFI CTRYAG 4 cut(s) 1329, 1573, 2655, 3319
BstSLI GKGCMC 2 cut(s) 2081, 2485
BstV2I GAAGAC 2 cut(s) 279, 2752
BstX2I RGATCY 2 cut(s) 2449, 2659
BstXI CCANNNNNNTGG 1 cut(s) 2932
BstYI RGATCY 2 cut(s) 2449, 2659
BstZ17I GTATAC 1 cut(s) 667
BsuI GTATCC 1 cut(s) 2576
BtsIMutI CAGTG 5 cut(s) 288, 387, 1531, 2433, 2541
BveI ACCTGC 1 cut(s) 944
Cac8I GCNNGC 3 cut(s) 1690, 2476, 3177
CciI TCATGA 1 cut(s) 1200
CfoI GCGC 2 cut(s) 576, 2112
Cfr13I GGNCC 2 cut(s) 905, 2714
Csp6I GTAC 5 cut(s) 42, 836, 2313, 2706, 2920
CviAII CATG 7 cut(s) 406, 1201, 1558, 1675, 1707, 2522, 3304
CviQI GTAC 5 cut(s) 42, 836, 2313, 2706, 2920
DdeI CTNAG 9 cut(s) 291, 363, 715, 797, 1473, 1615, 2136, 2259, 2337
DraI TTTAAA 1 cut(s) 2878
DriI GACNNNNNGTC 1 cut(s) 2914
EaeI YGGCCR 1 cut(s) 1690
Eam1104I CTCTTC 3 cut(s) 28, 1301, 2950
Eam1105I GACNNNNNGTC 1 cut(s) 2914
EarI CTCTTC 3 cut(s) 28, 1301, 2950
Eco130I CCWWGG 1 cut(s) 3151
Eco147I AGGCCT 2 cut(s) 802, 2176
Eco24I GRGCYC 1 cut(s) 798
Eco31I GGTCTC 1 cut(s) 1222
Eco32I GATATC 1 cut(s) 1198
Eco57I CTGAAG 5 cut(s) 264, 1214, 1282, 2238, 2933
Eco88I CYCGRG 3 cut(s) 839, 1381, 1419
EcoRI GAATTC 1 cut(s) 1082
EcoRII CCWGG 1 cut(s) 2946
EcoRV GATATC 1 cut(s) 1198
EcoT14I CCWWGG 1 cut(s) 3151
EcoT22I ATGCAT 4 cut(s) 601, 830, 2023, 2766
EcoT38I GRGCYC 1 cut(s) 798
ErhI CCWWGG 1 cut(s) 3151
FaeI CATG 7 cut(s) 409, 1204, 1561, 1678, 1710, 2525, 3307
FaqI GGGAC 1 cut(s) 3309
FatI CATG 7 cut(s) 405, 1200, 1557, 1674, 1706, 2521, 3303
FauI CCCGC 1 cut(s) 530
FauNDI CATATG 2 cut(s) 868, 3340
FbaI TGATCA 2 cut(s) 213, 2062
FblI GTMKAC 3 cut(s) 495, 666, 2223
FriOI GRGCYC 1 cut(s) 798
FspBI CTAG 8 cut(s) 45, 72, 372, 2273, 2387, 2645, 2750, 3102
FspI TGCGCA 1 cut(s) 575
GlaI GCGC 2 cut(s) 575, 2111
GsaI CCCAGC 1 cut(s) 852
GsuI CTGGAG 3 cut(s) 680, 1108, 2605
HapII CCGG 2 cut(s) 1538, 2090
HhaI GCGC 2 cut(s) 576, 2112
Hin1II CATG 7 cut(s) 409, 1204, 1561, 1678, 1710, 2525, 3307
Hin6I GCGC 2 cut(s) 574, 2110
HinP1I GCGC 2 cut(s) 574, 2110
HindIII AAGCTT 1 cut(s) 2442
HinfI GANTC 9 cut(s) 379, 541, 1148, 1310, 1379, 1541, 2209, 2279, 3014
HpaII CCGG 2 cut(s) 1538, 2090
HphI GGTGA 6 cut(s) 1212, 1388, 2488, 2696, 2861, 3118
Hpy99I CGWCG 1 cut(s) 914
HpyCH4III ACNGT 8 cut(s) 307, 760, 928, 1075, 1526, 1753, 1942, 2402
HpyCH4IV ACGT 3 cut(s) 912, 1244, 2317
HpyF3I CTNAG 9 cut(s) 291, 363, 715, 797, 1473, 1615, 2136, 2259, 2337
HpySE526I ACGT 3 cut(s) 912, 1244, 2317
Hsp92II CATG 7 cut(s) 409, 1204, 1561, 1678, 1710, 2525, 3307
HspAI GCGC 2 cut(s) 574, 2110
Kpn2I TCCGGA 1 cut(s) 1537
Ksp22I TGATCA 2 cut(s) 213, 2062
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 2 cut(s) 1120, 3172
LweI GCATC 8 cut(s) 456, 928, 1483, 2110, 2323, 2506, 2956, 3271
MaeI CTAG 8 cut(s) 45, 72, 372, 2273, 2387, 2645, 2750, 3102
MaeII ACGT 3 cut(s) 912, 1244, 2317
MaeIII GTNAC 8 cut(s) 301, 1021, 1126, 1376, 1546, 1608, 1756, 2508
MflI RGATCY 2 cut(s) 2449, 2659
MhlI GDGCHC 3 cut(s) 798, 2081, 2485
MlyI GAGTC 1 cut(s) 1373
MmeI TCCRAC 5 cut(s) 1173, 1257, 1772, 2137, 3142
Mph1103I ATGCAT 4 cut(s) 601, 830, 2023, 2766
MroI TCCGGA 1 cut(s) 1537
MroXI GAANNNNTTC 1 cut(s) 2415
MslI CAYNNNNRTG 3 cut(s) 1506, 1562, 2930
MspCI CTTAAG 1 cut(s) 692
MspI CCGG 2 cut(s) 1538, 2090
MspR9I CCNGG 2 cut(s) 2091, 2948
Mva1269I GAATGC 3 cut(s) 601, 1181, 2021
MvaI CCWGG 1 cut(s) 2948
NciI CCSGG 1 cut(s) 2091
NdeI CATATG 2 cut(s) 868, 3340
NlaIII CATG 7 cut(s) 409, 1204, 1561, 1678, 1710, 2525, 3307
NlaIV GGNNCC 1 cut(s) 907
NmeAIII GCCGAG 2 cut(s) 723, 1718
NmuCI GTSAC 3 cut(s) 301, 1376, 1756
NsbI TGCGCA 1 cut(s) 575
NsiI ATGCAT 4 cut(s) 601, 830, 2023, 2766
NspI RCATGY 2 cut(s) 409, 1678
NspV TTCGAA 1 cut(s) 2840
PaeR7I CTCGAG 1 cut(s) 1419
PagI TCATGA 1 cut(s) 1200
PceI AGGCCT 2 cut(s) 802, 2176
PciSI GCTCTTC 1 cut(s) 28
PctI GAATGC 3 cut(s) 601, 1181, 2021
PdmI GAANNNNTTC 1 cut(s) 2415
PfeI GAWTC 8 cut(s) 379, 541, 1148, 1310, 1541, 2209, 2279, 3014
PflMI CCANNNNNTGG 1 cut(s) 676
PleI GAGTC 1 cut(s) 1373
PpsI GAGTC 1 cut(s) 1373
Ppu21I YACGTR 1 cut(s) 2318
PshBI ATTAAT 2 cut(s) 3039, 3140
PsiI TTATAA 3 cut(s) 1554, 2828, 3192
Psp6I CCWGG 1 cut(s) 2946
PspFI CCCAGC 1 cut(s) 848
PspGI CCWGG 1 cut(s) 2946
PspN4I GGNNCC 1 cut(s) 907
PspPI GGNCC 2 cut(s) 905, 2714
PspXI VCTCGAGB 1 cut(s) 1419
PstI CTGCAG 1 cut(s) 3323
PsuI RGATCY 2 cut(s) 2449, 2659
RsaI GTAC 5 cut(s) 43, 837, 2314, 2707, 2921
RsaNI GTAC 5 cut(s) 42, 836, 2313, 2706, 2920
RseI CAYNNNNRTG 3 cut(s) 1506, 1562, 2930
SapI GCTCTTC 1 cut(s) 28
Sau96I GGNCC 2 cut(s) 905, 2714
ScaI AGTACT 1 cut(s) 837
SchI GAGTC 1 cut(s) 1373
ScrFI CCNGG 2 cut(s) 2091, 2948
SduI GDGCHC 3 cut(s) 798, 2081, 2485
SfaNI GCATC 8 cut(s) 456, 928, 1483, 2110, 2323, 2506, 2956, 3271
SfcI CTRYAG 4 cut(s) 1329, 1573, 2655, 3319
Sfr274I CTCGAG 1 cut(s) 1419
SfuI TTCGAA 1 cut(s) 2840
SlaI CTCGAG 1 cut(s) 1419
SmiMI CAYNNNNRTG 3 cut(s) 1506, 1562, 2930
SmlI CTYRAG 3 cut(s) 692, 704, 1419
SmoI CTYRAG 3 cut(s) 692, 704, 1419
SpeI ACTAGT 2 cut(s) 44, 71
SseBI AGGCCT 2 cut(s) 802, 2176
SsiI CCGC 5 cut(s) 224, 537, 1264, 3097, 3315
SspI AATATT 2 cut(s) 1438, 2821
SspMI CTAG 8 cut(s) 45, 72, 372, 2273, 2387, 2645, 2750, 3102
StuI AGGCCT 2 cut(s) 802, 2176
StyD4I CCNGG 2 cut(s) 2089, 2946
StyI CCWWGG 1 cut(s) 3151
TaaI ACNGT 8 cut(s) 307, 760, 928, 1075, 1526, 1753, 1942, 2402
TaiI ACGT 3 cut(s) 915, 1247, 2320
TaqI TCGA 5 cut(s) 544, 1420, 2302, 2409, 2840
TaqII GACCGA 1 cut(s) 2241
TatI WGTACW 4 cut(s) 41, 835, 2312, 2705
TauI GCSGC 3 cut(s) 226, 1267, 3318
TfiI GAWTC 8 cut(s) 379, 541, 1148, 1310, 1541, 2209, 2279, 3014
TscAI CASTG 5 cut(s) 288, 394, 1531, 2440, 2548
TseFI GTSAC 3 cut(s) 301, 1376, 1756
Tsp45I GTSAC 3 cut(s) 301, 1376, 1756
TspGWI ACGGA 1 cut(s) 3073
TspRI CASTG 5 cut(s) 288, 394, 1531, 2440, 2548
Van91I CCANNNNNTGG 1 cut(s) 676
Vha464I CTTAAG 1 cut(s) 692
VneI GTGCAC 1 cut(s) 2077
VspI ATTAAT 2 cut(s) 3039, 3140
XapI RAATTY 3 cut(s) 873, 1082, 2341
XceI RCATGY 2 cut(s) 409, 1678
XhoI CTCGAG 1 cut(s) 1419
XmiI GTMKAC 3 cut(s) 495, 666, 2223
XmnI GAANNNNTTC 1 cut(s) 2415
XspI CTAG 8 cut(s) 45, 72, 372, 2273, 2387, 2645, 2750, 3102
ZrmI AGTACT 1 cut(s) 837
Zsp2I ATGCAT 4 cut(s) 601, 830, 2023, 2766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.