pycom08g04580

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014408
Physical Location & Seq
Reverse (-)
3469087 .. 3472431
3345 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04580.1

Sequence Viewer

Length: 3345 bp
ATGGAATTGTTGATTGTCTGCTCTTCAAGCATGTGCTGTAGCAGCGTAAATTACAGTGTTGCTCTCACCGACAATAGGATATTTGCTATAAGCCACTTGGGTTCGCTGAAAGCTCGAAACTTTGGGAAGTTGAATGTTTGGGGATGTGGGTCTTTGTCAAACTTGACGAAAAAGCGAAAAAAAGGGATGGGTTTTTGTGGGTTTGTGATGAAAAGGTCAGAGGAGGAGGTGGTGGTGGGGAAGAAAAACCTGAAAATTTCGGTTTCTTCTGAGGAAGTCATGAGGGTTTTGAAGTCAGTTTCGGACCCGAAATCTGCTTTAGCATTGTTTAAGTCATTTGCGGAGTTGCCTAGTGTTGTTCACACCACTGAAACTTGCAATTACATGCTTGAAGTCTTGGGGGTTGATAGGAGAGTGGAGGATATCGCTTATGTGTTTGATTTGATGCAGAAGCAGATCATAAATAGGAGTTTGGACACTTACCTTACTATCTTTGAGGGTCTTGATGTACGAGGTGGTATTCGACAGGCGCCCTTCGCTCTTGAAGTGATGCGAAAAGCGGGGTTTGTATTGAATGCGTATTCGTACAATGGGTTGATCTATAATCTTATTCAGTCTGGATATTGTAGGGAGGCTTTGGAGGTTTATGAAAGAGTGGTTTCAGAAGGGATTAAGCCAAGTCTGAAGACTTACTCGGCACTTATGGTGGCATTGGGGAAAAGAAGGGATGTTAAAACTGTTATGAGCTTGCTAAATGAGATGGAAATTTTGGGATTGAGGCCTAATGTTTATACTTTTACTATATGCATTAGAGTACTTGGGAGGGCAGGGAAAATTGACGAAGCGTATGAGATTTTTAAGAGAATGGATGATGAGGGATGTGGCCCCGATGTTGTGACTTACACCGTTCTCATTGATGCTCTTTGTAATGCAGGTAAACTTGACAAGGCTAAGGAGTTGTTTGTAAAAATGAAAGCCAGTGGTCATAAGCCTGACCAAGTAACCTACATTACTTTGCTGGATAAGTTTAGTGACGGTAAAGACTTGGACACAGTGAAAGAATTTTGGAGTGAAATGGAAGCTGACGGTTATGCTCCTGATGTGGTTACTTTCACCATACTTGTCAATGCTTTATGCAAAGCTGGAAATGTTGACGAAGCATTTAATATGTTGGATACCATGAGAAAACAAGGGGTCTCGCCGAATCTTCATACATACAACACTTTAATTGGTGGACTTCTGCAGTTATGTAGGTTGGACGAGGCATTGAAGCTTTTCAATGGTATTGACTGTCTGGGTGTTCCACCTACTGCTTACACATACATCCTTTTCATTGACTACTATGGAAAGTGTGGCGACTCTGGCAAAGCTATGGAGGCCTTTGAGAAGATGAAAAATAAAGGCATTGTTCCTAATATTGTTGCTTGTAATGCATCTTTGTACAGTCTTGCTGAAGAGGGTAGACTTCAAGAGGCGCAGGATGTTTATAATGAGCTCAAATATTACGGGCTTTCTCCAGATTCAGTAACTTACAACATGATGATGAAGTGCTATAGTAAGGTGGGGCAAATAGATGACGCCATCGAGTTACTCTTGGAGATGGAAAGAAATGGGTGCGAAGCAGATGTAATTATTGTTAATTCTTTGATTGACATGCTTTACAAGGCTGACCGAGTGGATGAAGCATGGCAAATGTTTTACAGAATGAAGGAAATGAAGCTCACTCCAACAGTTGTGACCTACAACACATTACTCGCTGCATTAGGGAAAGATGGAGAAGTCAAAAAGGCCATTGAAATGTTTGAGAATATGGCCGAACAAGGGTGTCCTCCAAACACAATAACTTTCAACACACTCCTGAACTGCCTTTGCAAGAATGACGAGGTTACTCTGGCCTTGAAAATGTTCTGCAAAATGACCACAATGAGTTGCAGTCCTGACGTTCTGACCTATAATACTATACTTTACGGCTTAATCAGAGAAAACAGAATTGATTATGCATTCTGGTTCTTCCATCAGATGAAGAAATCACTACTTCCTGATCATATAACATTGTGTACCCTCCTACCTGGGGTGGTAAAGGATGGGCGTATTGAGGATGCTTTCAAAATTGCTGAGAACTTCGTGTATCAGGTTGGGGTACGAGCAGATAGGCCTTTTTGGGAAGATTTAATGGGAGGGATTTTGATTGAAGCTGAAATAGACAGAGCCATTTTATTTGCTGAAAGGTTGATCTCGGATAGGATTTGCCGGGATGACTCTGTACTGATTCCTCTACTTAGAGTTTTGTGTACGCAAAGGAAAGCTTTTGATGCGCATAAGGTATTTGAGAAATTTATGAAAACTCTTGGAATTAAGCCAACACTAGAAGCATACAACTACTTGATTGAATGGCTTCTGAAAGATCATGCCGCTGAAAGAGCTTGGGATCTATTTAAGGAGATGAAGAATGGTGGTTGTACCCCCGATGTATTCACCTATAACTTGTTCCTTGATGCTCATGGGAAGTCTGGGAACATCACCGAACTCTTTGAATTGTACGAGGAGATGATTTTTAGGGGATGCAAGCCTAACACCGTAACGCACAATATAGTCATCTCCAGTCTTGTGAAATCTGATGGCGTGGATAGTGCTATAGATTTGTATTATGATCTCGTAAGTGGTGATTTCTCTCCCTCTCCTTGTACATATGGTCCTCTCATAGATGGACTTTTCAAGTCAGGAAGACTAGACGAAGCAATGCATTTCTTTGATGAGATGGCCGACTATGGATGCAAACCTAATTCTGCTATTTTTAATATTCTTATAAATGGGTTTGCGAAAACAGGTGATGTGGAAGCTGCCTGTGACTTATTTAGAAGGATGATTAAAGAAGGGATAAGACCTGACCTGAAGTCTTACACCATTCTTGTGGATTGCCTCTGCCAGGCAGGGAGAGTGGATGATGCCATGGACTATTTTGAGGAGTTAAAACAAAGTGGCCTCGATCCTGACTCGGTTTCTTACAACCTTATGATAAATGGACTTGGAAGATCACGGAGGGTAGAGGAAGCACTCACTCTTTATGATGAAATGCGAACTAGAGGAATCGCTCCTGATCTTTACACATACAATTTGCTAATACTCAATCTCGGACTTGTTGGAATGGTGGAGCAAGCTGGGAGGATTTATGAAGAACTGCAACTTGTAGGTCTTGAACCTGATGTGTTTACCTACAATGCTCTCATTCGACTGTATAGCACATCCGGGGATCCAGATCATGCTTATGCAGTTTACAAGAACATGATGGTTGATGGCTGCAGCCCCAATGTAGGAACATTTGCACAGCTTCCCAACCAAACTTGA

Protein Analysis

1115

Amino Acids

125.32

Weight (kDa)

5.16

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 180 - 289 5.9e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 183 - 241 1.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 192 - 238 9e-06 PPR repeat family
PPR_long PF17177 211 - 341 3.6e-12 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 230 - 273 1.3e-08 PPR repeat family
PPR_3 PF13812 249 - 307 2.4e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 258 - 289 2.7e-07 PPR repeat
PPR PF01535 264 - 294 2.4e-06 PPR repeat
PPR_2 PF13041 268 - 310 4.7e-12 PPR repeat family
PPR_1 PF12854 293 - 325 3.3e-13 PPR repeat
PPR_2 PF13041 296 - 343 7.8e-16 PPR repeat family
PPR_3 PF13812 296 - 342 5.7e-07 Pentatricopeptide repeat domain
PPR PF01535 299 - 328 2e-08 PPR repeat
PPR_2 PF13041 338 - 380 2.6e-07 PPR repeat family
PPR_long PF17177 348 - 453 3e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 357 - 411 1.5e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 363 - 395 4.7e-14 PPR repeat
PPR_2 PF13041 366 - 413 1.3e-17 PPR repeat family
PPR PF01535 369 - 399 3.8e-07 PPR repeat
PPR_2 PF13041 405 - 445 2.5e-08 PPR repeat family
PPR_3 PF13812 461 - 519 3.7e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 471 - 520 3.6e-11 PPR repeat family
TPR_24 PF23276 482 - 603 5.4e-09 Fungal tetratrico peptide repeats
PPR_1 PF12854 503 - 535 4.7e-08 PPR repeat
PPR_2 PF13041 506 - 543 1.4e-10 PPR repeat family
PPR PF01535 509 - 539 1.5e-08 PPR repeat
PPR_long PF17177 525 - 613 3.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 530 - 590 2.3e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 542 - 589 8.8e-12 PPR repeat family
PPR_1 PF12854 574 - 604 6e-09 PPR repeat
PPR_2 PF13041 576 - 625 4.6e-18 PPR repeat family
PPR PF01535 579 - 609 6.9e-07 PPR repeat
PPR_3 PF13812 600 - 655 1.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 608 - 640 1.1e-09 PPR repeat
PPR_2 PF13041 611 - 659 3.2e-14 PPR repeat family
PPR_1 PF12854 643 - 675 8.7e-07 PPR repeat
PPR_2 PF13041 646 - 693 3.4e-08 PPR repeat family
PPR_2 PF13041 789 - 834 3.7e-13 PPR repeat family
PPR_3 PF13812 811 - 870 1.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 822 - 871 7.7e-14 PPR repeat family
PPR_1 PF12854 890 - 920 2e-08 PPR repeat
PPR_2 PF13041 892 - 929 3.5e-08 PPR repeat family
PPR_long PF17177 911 - 1002 4.3e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 924 - 955 8.4e-09 PPR repeat
PPR_2 PF13041 927 - 976 1.6e-15 PPR repeat family
PPR PF01535 931 - 960 2.2e-07 PPR repeat
TPR_24 PF23276 932 - 1024 8.7e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 951 - 1010 1.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 959 - 990 5.2e-09 PPR repeat
PPR PF01535 966 - 995 9.4e-07 PPR repeat
PPR_2 PF13041 966 - 1010 3.7e-12 PPR repeat family
PPR_1 PF12854 994 - 1026 1.6e-08 PPR repeat
PPR_2 PF13041 997 - 1041 1.7e-15 PPR repeat family
PPR PF01535 1000 - 1030 9.2e-09 PPR repeat
PPR_long PF17177 1003 - 1108 5e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 1055 - 1108 3e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 1067 - 1107 4.6e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014408)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31850
fragaria_vesca FvH4_2g41180 FvH4_2g41180
malus_domestica MD08G1055800.v1.1
prunus_persica Prupe.1G394600_v2.0.a1 Prupe.1G394600_v2.0.a1
pyrus_communis pycom08g04580
rosa_chinensis RchiOBHm_Chr6g0299351
rosa_laevigata RLG00000011440
rosa_multiflora Rmu_ssc0000374.1_g000003
rosa_roxburghii Rroxscaffold_7G00168770
rosa_rugosa Rorug06G0287500
rosa_samantha Rh6AG399000 Rh6BG407400 Rh6CG413300 Rh6DG400200
rosa_wichuraiana Rw6G034930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1488, 2807
Acc16I TGCGCA 1 cut(s) 2316
Acc36I ACCTGC 1 cut(s) 923
AccB1I GGYRCC 1 cut(s) 529
AccI GTMKAC 1 cut(s) 1462
AciI CCGC 3 cut(s) 341, 560, 2412
AclWI GGATC 4 cut(s) 2436, 2981, 3245, 3258
AcoI YGGCCR 2 cut(s) 1812, 2760
AcsI RAATTY 4 cut(s) 255, 765, 1061, 2333
AcuI CTGAAG 3 cut(s) 704, 1473, 2912
AcyI GRCGYC 2 cut(s) 530, 1578
AfiI CCNNNNNNNGG 5 cut(s) 75, 1821, 2071, 2926, 3311
AhdI GACNNNNNGTC 1 cut(s) 2893
AjnI CCWGG 2 cut(s) 2068, 2925
Alw21I GWGCWC 1 cut(s) 1497
Alw26I GTCTC 1 cut(s) 1201
AlwI GGATC 4 cut(s) 2436, 2981, 3245, 3258
AoxI GGCC 9 cut(s) 779, 883, 1377, 1788, 1812, 1893, 2153, 2760, 2980
ApeKI GCWGC 5 cut(s) 42, 1757, 2840, 3297, 3300
ApoI RAATTY 4 cut(s) 255, 765, 1061, 2333
Asp700I GAANNNNTTC 3 cut(s) 1274, 1904, 2394
AspLEI GCGC 3 cut(s) 532, 1477, 2317
AspS9I GGNCC 3 cut(s) 304, 884, 2693
AsuC2I CCSGG 2 cut(s) 2252, 3247
AsuHPI GGTGA 6 cut(s) 58, 1105, 2467, 2512, 2675, 2840
AvaII GGWCC 2 cut(s) 304, 2693
BamHI GGATCC 1 cut(s) 3250
BanI GGYRCC 1 cut(s) 529
BanII GRGCYC 1 cut(s) 1497
BbsI GAAGAC 2 cut(s) 692, 2731
Bbv12I GWGCWC 1 cut(s) 1497
BbvI GCAGC 5 cut(s) 54, 1744, 2827, 3284, 3312
BceAI ACGGC 1 cut(s) 1984
BciT130I CCWGG 2 cut(s) 2070, 2927
BciVI GTATCC 1 cut(s) 1168
BclI TGATCA 1 cut(s) 2041
BcnI CCSGG 2 cut(s) 2252, 3247
BcoDI GTCTC 1 cut(s) 1201
BfaI CTAG 4 cut(s) 351, 2366, 2729, 3081
BfmI CTRYAG 5 cut(s) 37, 1241, 1552, 2634, 3298
BfoI RGCGCY 1 cut(s) 533
BfuAI ACCTGC 1 cut(s) 923
BfuI GTATCC 1 cut(s) 1168
BisI GCNGC 6 cut(s) 43, 1758, 2412, 2841, 3298, 3301
BlsI GCNGC 6 cut(s) 44, 1759, 2413, 2842, 3299, 3302
BmcAI AGTACT 1 cut(s) 816
Bme1390I CCNGG 4 cut(s) 2070, 2252, 2927, 3247
Bme18I GGWCC 2 cut(s) 304, 2693
BmeRI GACNNNNNGTC 1 cut(s) 2893
BmgT120I GGNCC 3 cut(s) 304, 884, 2693
BmiI GGNNCC 4 cut(s) 306, 531, 886, 3252
BmrFI CCNGG 4 cut(s) 2070, 2252, 2927, 3247
BpiI GAAGAC 2 cut(s) 692, 2731
BpmI CTGGAG 2 cut(s) 1500, 2584
Bpu10I CCTNAGC 1 cut(s) 951
BpuMI CCSGG 2 cut(s) 2252, 3247
BsaBI GATNNNNATC 1 cut(s) 3255
BsaHI GRCGYC 2 cut(s) 530, 1578
BsaI GGTCTC 1 cut(s) 1201
BsaJI CCNNGG 3 cut(s) 2069, 2949, 3246
Bsc4I CCNNNNNNNGG 5 cut(s) 75, 1821, 2071, 2926, 3311
Bse1I ACTGG 2 cut(s) 978, 2601
Bse3DI GCAATG 1 cut(s) 2745
Bse8I GATNNNNATC 1 cut(s) 3255
BseBI CCWGG 2 cut(s) 2070, 2927
BseDI CCNNGG 3 cut(s) 2069, 2949, 3246
BseJI GATNNNNATC 1 cut(s) 3255
BseLI CCNNNNNNNGG 5 cut(s) 75, 1821, 2071, 2926, 3311
BseMI GCAATG 1 cut(s) 2745
BseMII CTCAG 2 cut(s) 261, 2106
BseNI ACTGG 2 cut(s) 978, 2601
BseRI GAGGAG 4 cut(s) 236, 239, 2558, 2978
BseXI GCAGC 5 cut(s) 54, 1744, 2827, 3284, 3312
BseYI CCCAGC 1 cut(s) 3158
BshFI GGCC 9 cut(s) 781, 885, 1379, 1790, 1814, 1895, 2155, 2762, 2982
BshNI GGYRCC 1 cut(s) 529
BsiHKAI GWGCWC 1 cut(s) 1497
BsiSI CCGG 2 cut(s) 2251, 3246
BslI CCNNNNNNNGG 5 cut(s) 75, 1821, 2071, 2926, 3311
BsmAI GTCTC 1 cut(s) 1201
BsmI GAATGC 2 cut(s) 580, 2000
BsnI GGCC 9 cut(s) 781, 885, 1379, 1790, 1814, 1895, 2155, 2762, 2982
Bso31I GGTCTC 1 cut(s) 1201
Bsp1286I GDGCHC 1 cut(s) 1497
Bsp1407I TGTACA 2 cut(s) 1440, 2684
Bsp19I CCATGG 1 cut(s) 2949
BspACI CCGC 3 cut(s) 341, 560, 2412
BspANI GGCC 9 cut(s) 781, 885, 1379, 1790, 1814, 1895, 2155, 2762, 2982
BspCNI CTCAG 2 cut(s) 262, 2107
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 4 cut(s) 306, 531, 886, 3252
BspMAI CTGCAG 2 cut(s) 1245, 3302
BspMI ACCTGC 1 cut(s) 923
BspPI GGATC 4 cut(s) 2436, 2981, 3245, 3258
BspQI GCTCTTC 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 529
BspTNI GGTCTC 1 cut(s) 1201
BsrDI GCAATG 1 cut(s) 2745
BsrGI TGTACA 2 cut(s) 1440, 2684
BsrI ACTGG 2 cut(s) 978, 2601
BssECI CCNNGG 3 cut(s) 2069, 2949, 3246
BssNI GRCGYC 2 cut(s) 530, 1578
BssT1I CCWWGG 1 cut(s) 2949
Bst2UI CCWGG 2 cut(s) 2070, 2927
Bst6I CTCTTC 2 cut(s) 28, 1449
BstACI GRCGYC 2 cut(s) 530, 1578
BstAUI TGTACA 2 cut(s) 1440, 2684
BstC8I GCNNGC 3 cut(s) 749, 2567, 3156
BstDEI CTNAG 4 cut(s) 270, 951, 2115, 2279
BstDSI CCRYGG 1 cut(s) 2949
BstENI CCTNNNNNAGG 1 cut(s) 2924
BstH2I RGCGCY 1 cut(s) 533
BstHHI GCGC 3 cut(s) 532, 1477, 2317
BstMAI GTCTC 1 cut(s) 1201
BstMWI GCNNNNNNNGC 8 cut(s) 27, 42, 536, 1362, 1376, 1430, 2312, 2420
BstNI CCWGG 2 cut(s) 2070, 2927
BstNSI RCATGY 3 cut(s) 34, 388, 1657
BstSCI CCNGG 4 cut(s) 2068, 2250, 2925, 3245
BstSFI CTRYAG 5 cut(s) 37, 1241, 1552, 2634, 3298
BstV1I GCAGC 5 cut(s) 54, 1744, 2827, 3284, 3312
BstV2I GAAGAC 2 cut(s) 692, 2731
BstX2I RGATCY 2 cut(s) 2428, 3250
BstXI CCANNNNNNTGG 1 cut(s) 2911
BstYI RGATCY 2 cut(s) 2428, 3250
BsuI GTATCC 1 cut(s) 1168
BsuRI GGCC 9 cut(s) 781, 885, 1379, 1790, 1814, 1895, 2155, 2762, 2982
BtgI CCRYGG 1 cut(s) 2949
BtsIMutI CAGTG 4 cut(s) 61, 366, 985, 1059
BveI ACCTGC 1 cut(s) 923
Cac8I GCNNGC 3 cut(s) 749, 2567, 3156
CciI TCATGA 1 cut(s) 279
CfoI GCGC 3 cut(s) 532, 1477, 2317
Cfr13I GGNCC 3 cut(s) 304, 884, 2693
CseI GACGC 1 cut(s) 1586
DdeI CTNAG 4 cut(s) 270, 951, 2115, 2279
DinI GGCGCC 1 cut(s) 531
DriI GACNNNNNGTC 1 cut(s) 2893
EaeI YGGCCR 2 cut(s) 1812, 2760
Eam1104I CTCTTC 2 cut(s) 28, 1449
Eam1105I GACNNNNNGTC 1 cut(s) 2893
EarI CTCTTC 2 cut(s) 28, 1449
Ecl136II GAGCTC 1 cut(s) 1495
Eco130I CCWWGG 1 cut(s) 2949
Eco147I AGGCCT 3 cut(s) 781, 1379, 2155
Eco24I GRGCYC 1 cut(s) 1497
Eco31I GGTCTC 1 cut(s) 1201
Eco32I GATATC 1 cut(s) 424
Eco47I GGWCC 2 cut(s) 304, 2693
Eco53kI GAGCTC 1 cut(s) 1495
Eco57I CTGAAG 3 cut(s) 704, 1473, 2912
EcoICRI GAGCTC 1 cut(s) 1495
EcoNI CCTNNNNNAGG 1 cut(s) 2924
EcoRII CCWGG 2 cut(s) 2068, 2925
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 2949
EcoT22I ATGCAT 4 cut(s) 809, 1435, 2002, 2745
EcoT38I GRGCYC 1 cut(s) 1497
EgeI GGCGCC 1 cut(s) 531
EheI GGCGCC 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 2949
FalI AAGNNNNNCTT 2 cut(s) 2290, 2322
FauI CCCGC 1 cut(s) 553
FauNDI CATATG 1 cut(s) 2689
FbaI TGATCA 1 cut(s) 2041
FblI GTMKAC 1 cut(s) 1462
Fnu4HI GCNGC 6 cut(s) 43, 1758, 2412, 2841, 3298, 3301
FriOI GRGCYC 1 cut(s) 1497
Fsp4HI GCNGC 6 cut(s) 43, 1758, 2412, 2841, 3298, 3301
FspAI RTGCGCAY 1 cut(s) 2316
FspBI CTAG 4 cut(s) 351, 2366, 2729, 3081
FspI TGCGCA 1 cut(s) 2316
GlaI GCGC 3 cut(s) 531, 1476, 2316
GluI GCNGC 6 cut(s) 43, 1758, 2412, 2841, 3298, 3301
GsaI CCCAGC 1 cut(s) 3162
GsuI CTGGAG 2 cut(s) 1500, 2584
HaeII RGCGCY 1 cut(s) 533
HaeIII GGCC 9 cut(s) 781, 885, 1379, 1790, 1814, 1895, 2155, 2762, 2982
HapII CCGG 2 cut(s) 2251, 3246
HgaI GACGC 1 cut(s) 1586
HhaI GCGC 3 cut(s) 532, 1477, 2317
Hin1I GRCGYC 2 cut(s) 530, 1578
Hin6I GCGC 3 cut(s) 530, 1475, 2315
HinP1I GCGC 3 cut(s) 530, 1475, 2315
HincII GTYRAC 1 cut(s) 1153
HindII GTYRAC 1 cut(s) 1153
HindIII AAGCTT 2 cut(s) 1271, 2304
HinfI GANTC 7 cut(s) 1204, 1358, 1520, 2258, 2269, 2993, 3087
HpaII CCGG 2 cut(s) 2251, 3246
HphI GGTGA 6 cut(s) 58, 1105, 2467, 2512, 2675, 2840
Hpy166II GTNNAC 9 cut(s) 361, 938, 1153, 1235, 1463, 2058, 2292, 3210, 3274
Hpy8I GTNNAC 9 cut(s) 361, 938, 1153, 1235, 1463, 2058, 2292, 3210, 3274
HpyAV CCTTC 6 cut(s) 544, 659, 717, 1702, 2853, 2867
HpyCH4IV ACGT 1 cut(s) 1941
HpyF10VI GCNNNNNNNGC 8 cut(s) 27, 42, 536, 1362, 1376, 1430, 2312, 2420
HpyF3I CTNAG 4 cut(s) 270, 951, 2115, 2279
HpySE526I ACGT 1 cut(s) 1941
Hsp92I GRCGYC 2 cut(s) 530, 1578
HspAI GCGC 3 cut(s) 530, 1475, 2315
KasI GGCGCC 1 cut(s) 529
Ksp22I TGATCA 1 cut(s) 2041
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 3 cut(s) 1099, 3097, 3151
Lsp1109I GCAGC 5 cut(s) 54, 1744, 2827, 3284, 3312
MaeI CTAG 4 cut(s) 351, 2366, 2729, 3081
MaeII ACGT 1 cut(s) 1941
MflI RGATCY 2 cut(s) 2428, 3250
MhlI GDGCHC 1 cut(s) 1497
Mly113I GGCGCC 1 cut(s) 530
MlyI GAGTC 3 cut(s) 1352, 2252, 2987
MmeI TCCRAC 4 cut(s) 1152, 1236, 1751, 3121
Mph1103I ATGCAT 4 cut(s) 809, 1435, 2002, 2745
MroXI GAANNNNTTC 3 cut(s) 1274, 1904, 2394
MslI CAYNNNNRTG 4 cut(s) 1541, 1796, 2909, 3264
MspA1I CMGCKG 1 cut(s) 2414
MspI CCGG 2 cut(s) 2251, 3246
MspR9I CCNGG 4 cut(s) 2070, 2252, 2927, 3247
Mva1269I GAATGC 2 cut(s) 580, 2000
MvaI CCWGG 2 cut(s) 2070, 2927
MwoI GCNNNNNNNGC 8 cut(s) 27, 42, 536, 1362, 1376, 1430, 2312, 2420
NarI GGCGCC 1 cut(s) 530
NciI CCSGG 2 cut(s) 2252, 3247
NcoI CCATGG 1 cut(s) 2949
NdeI CATATG 1 cut(s) 2689
NlaIV GGNNCC 4 cut(s) 306, 531, 886, 3252
NmeAIII GCCGAG 1 cut(s) 674
NmuCI GTSAC 4 cut(s) 895, 1031, 1735, 2846
NsbI TGCGCA 1 cut(s) 2316
NsiI ATGCAT 4 cut(s) 809, 1435, 2002, 2745
NspI RCATGY 3 cut(s) 34, 388, 1657
PagI TCATGA 1 cut(s) 279
PceI AGGCCT 3 cut(s) 781, 1379, 2155
PciSI GCTCTTC 1 cut(s) 28
PctI GAATGC 2 cut(s) 580, 2000
PdmI GAANNNNTTC 3 cut(s) 1274, 1904, 2394
PfeI GAWTC 4 cut(s) 1204, 1520, 2269, 3087
PkrI GCNGC 6 cut(s) 44, 1759, 2413, 2842, 3299, 3302
PleI GAGTC 3 cut(s) 1352, 2252, 2987
PluTI GGCGCC 1 cut(s) 533
PpsI GAGTC 3 cut(s) 1352, 2252, 2987
PsiI TTATAA 2 cut(s) 1488, 2807
Psp124BI GAGCTC 1 cut(s) 1497
Psp6I CCWGG 2 cut(s) 2068, 2925
PspFI CCCAGC 1 cut(s) 3158
PspGI CCWGG 2 cut(s) 2068, 2925
PspN4I GGNNCC 4 cut(s) 306, 531, 886, 3252
PspPI GGNCC 3 cut(s) 304, 884, 2693
PstI CTGCAG 2 cut(s) 1245, 3302
PsuI RGATCY 2 cut(s) 2428, 3250
RseI CAYNNNNRTG 4 cut(s) 1541, 1796, 2909, 3264
SacI GAGCTC 1 cut(s) 1497
SapI GCTCTTC 1 cut(s) 28
SatI GCNGC 6 cut(s) 43, 1758, 2412, 2841, 3298, 3301
Sau96I GGNCC 3 cut(s) 304, 884, 2693
ScaI AGTACT 1 cut(s) 816
SchI GAGTC 3 cut(s) 1352, 2252, 2987
ScrFI CCNGG 4 cut(s) 2070, 2252, 2927, 3247
SduI GDGCHC 1 cut(s) 1497
SfcI CTRYAG 5 cut(s) 37, 1241, 1552, 2634, 3298
SfoI GGCGCC 1 cut(s) 531
SinI GGWCC 2 cut(s) 304, 2693
SmiMI CAYNNNNRTG 4 cut(s) 1541, 1796, 2909, 3264
SseBI AGGCCT 3 cut(s) 781, 1379, 2155
SsiI CCGC 3 cut(s) 341, 560, 2412
SspDI GGCGCC 1 cut(s) 529
SspI AATATT 3 cut(s) 1417, 1502, 2800
SspMI CTAG 4 cut(s) 351, 2366, 2729, 3081
SstI GAGCTC 1 cut(s) 1497
StuI AGGCCT 3 cut(s) 781, 1379, 2155
StyD4I CCNGG 4 cut(s) 2068, 2250, 2925, 3245
StyI CCWWGG 1 cut(s) 2949
TaiI ACGT 1 cut(s) 1944
TaqI TCGA 5 cut(s) 115, 523, 1584, 2985, 3229
TaqII GACCGA 1 cut(s) 1686
TatI WGTACW 4 cut(s) 814, 1440, 2263, 2684
TauI GCSGC 1 cut(s) 2414
TfiI GAWTC 4 cut(s) 1204, 1520, 2269, 3087
TscAI CASTG 4 cut(s) 61, 373, 985, 1059
TseFI GTSAC 4 cut(s) 895, 1031, 1735, 2846
TseI GCWGC 5 cut(s) 42, 1757, 2840, 3297, 3300
Tsp45I GTSAC 4 cut(s) 895, 1031, 1735, 2846
TspGWI ACGGA 1 cut(s) 3052
TspRI CASTG 4 cut(s) 61, 373, 985, 1059
VpaK11BI GGWCC 2 cut(s) 304, 2693
XagI CCTNNNNNAGG 1 cut(s) 2924
XapI RAATTY 4 cut(s) 255, 765, 1061, 2333
XceI RCATGY 3 cut(s) 34, 388, 1657
XmiI GTMKAC 1 cut(s) 1462
XmnI GAANNNNTTC 3 cut(s) 1274, 1904, 2394
XspI CTAG 4 cut(s) 351, 2366, 2729, 3081
ZrmI AGTACT 1 cut(s) 816
Zsp2I ATGCAT 4 cut(s) 809, 1435, 2002, 2745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.