AT4G32350

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009820
Physical Location & Seq
Forward (+)
15617361 .. 15621062
3702 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32350.2

Sequence Viewer

Length: 2199 bp
ATGTTTGATGGTTTCTTAGGCCGCGGATTTGCTCCCAAGGGAAAACCACTGATAAAACTGACTAAGAATCGGATCGATGTTCTCCGGAGGAAAAGGAATGCTACGATAAAGTTTCTGAAGAGAGATTTGGCTGATCTGATCATTAACGGTCATGACTACAATGCTTTTAGCAGGGCAGGAGGGCTTCTTGATGAACTAAGGTATCTCTGGAGTCTCGACTTTGTCGAGCAGACCTGTGATTTCGTATACAAGCAGCTCTCTACCATGCAAAAGACGCCGGAATGTCCAGAAGATTGCCGTGAAGCTATCTCCTCGTTAATGTTTGCAGCCTCTGGATTTTCCGAGTTGCCTGAGCTGCGTGAATTGAGGCAAATGTTTCACGAAAAATACACAGATTCACTTGCATTGTTTGTCAACCAGGAGCTTGTTGAGAATATGTCATCCAAGCCCTTTTCTATGGAGAAGAAAGTGAAGTTAATGGAAGATGTTGCATTGGAGTTCTCTATTCGTTGGGATTCTAAAGATTTCGAGAAGAGGATTGTGAGACAGAATAGTATTTCAGTAATGGAGACCCCAAAAAGCACCAACGACAAGTATAAGCCTGTAGACAGAAACATGGCCTTGCCAAAAAGAGAAGAGTTTGAAGGTTCTGAAAATGGGGTTAGCTTGAACAGAAAAACGGCTGAGGCATCAGAAAGAAGAGATCCTTTGTTTCAATCTGATAAAGAAAGTTATCAGAATGGTCTCAGAGGAAATCAGCGTGGGCTAACATATAAAGAAAGGTCAGAGAATGTTCTTCACGCCTCTAGATCGGAAAGCAAAGACAATAAGGCCGAAAGGAAAGAGTTTTATCTGCATTCTAAGCAAAACCCTGCTAGAGAAAAACATCAGCCTATTTTTAACGAAGGTGACACTATAGTGATGAAGGTCAACTATGGGAATCTCGGTCAAGGGAATGGACATCGACCTGGTGTAGTTGATGCGCATAAGAAAACCGAGTTTGTTGCCTCAGAAAGGAAAGAGTTTTATTCGCAATCTAAGCAAGAACCTTCTAGAGAAAGACATCAACCTATTTTTAATGATGGTGACACTATTGTAATGAAGGTCAAGCATGAGAACCACGTTCAAGGGAATGGACATAAAAATGGTGTAGTTGATCTGCATAAGAAAATAGAGGTTAATGCTTCAGAGAAACTTAAGTCTAGCAGCAGCAAAAGAGCTGACAAGTTAGTTATCGGCTTTAAACAAGAGAGTTTCTTTCAAGGGTACAAACATGAGAAAAATGAAGAGCATGCACATCAGAAAGTGGAAGATAGTACCTCAAGACCTCCGAAGCCAAATAGCAAGTCAAAGAGAGCGGAATCTATCAATCCAGGTTCGAGACATCATAATGATCGGGAGAGTAAAGAAAATGCAGTTCTTGTGGGAAAGAGCACCGAAGAAGATCCATCTGGTGATAATGTTAAGGGTGGAGAATACGAATATGATCATGCAAATCCAGCAAGGAAAGTTGAGGAGCGAGAAACAGAGAGAATGAAATCACCCTTCTACAAATCCCTTCCACCTCCGTATGTTAAAAAATCTATTGCTAAAGCAAGGCATGAAAAAGCTGAAGCTTTAGATAATCCAAAGGCTCGTTTTGATGGTGAAGAAGGAAATCATCCAGACAATGGTAAAAATGTTTATGGGGCAGAGAGACGAAATGGAGCAGGTCATCATGAAGTGAATGACATAGACAATGCATCTCTGAAACGGCAAACTAACAGAAGAAAGCACATTGTAGAATCGGGAGGTGATGATCACATAAGTAGCCGAAGAAGGGAAAACTCAAGGAAAGGCCTTCAAGTCTTAATTGACGAGGATGAGAAAGACAGTGAGGAGAAGATGATGGATAAACTTCTGATGCATTACAGTAAAAAACCTTCAAGCTATGAAAAGGACAATGTGCAAGAAGAATCCAAAAGCAGACGTACCCATCTTAAAAAAGGTGAATCTGATGAAGAGATGATGATTCATCAGCCGGCTCGGTCACGGTCTCTACCCGCTGAACAGTTGGCCGGGCCATCTGAACCTGCAAAAACATTTGCCCGTGCAGCCTCGTTTCAGCCAGAACGTTCCAGTGAAGCTAAGCACGTTCACCCCAAGTTACCAAACTATGACGATTTGGCTGCCAGATTTGCAGAGCTAAAAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

732

Amino Acids

83.97

Weight (kDa)

9.04

Isoelectric Point (pI)

53.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 1 - 81 7e-17 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 984
Acc36I ACCTGC 2 cut(s) 1700, 2080
AccB7I CCANNNNNTGG 1 cut(s) 1670
AccBSI CCGCTC 1 cut(s) 1358
AccI GTMKAC 2 cut(s) 246, 606
AccII CGCG 1 cut(s) 24
AccIII TCCGGA 1 cut(s) 84
AciI CCGC 4 cut(s) 22, 24, 1358, 2043
AclI AACGTT 1 cut(s) 2113
AclWI GGATC 3 cut(s) 80, 698, 1439
AcoI YGGCCR 1 cut(s) 2055
AcuI CTGAAG 3 cut(s) 137, 1170, 1632
AcyI GRCGYC 1 cut(s) 275
AfaI GTAC 3 cut(s) 1268, 1318, 1972
AfiI CCNNNNNNNGG 3 cut(s) 1014, 1670, 1819
AflII CTTAAG 1 cut(s) 1196
AgsI TTSAA 7 cut(s) 644, 670, 716, 1127, 1262, 1844, 1926
AjnI CCWGG 3 cut(s) 417, 967, 1372
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AleI CACNNNNGTG 1 cut(s) 917
Alw21I GWGCWC 1 cut(s) 1436
Alw26I GTCTC 7 cut(s) 218, 538, 563, 749, 1375, 1690, 2040
AlwI GGATC 3 cut(s) 80, 698, 1439
AlwNI CAGNNNCTG 1 cut(s) 332
Aor13HI TCCGGA 1 cut(s) 84
AoxI GGCC 6 cut(s) 19, 618, 831, 1837, 2055, 2060
ApeKI GCWGC 7 cut(s) 253, 326, 355, 1206, 1209, 2093, 2168
ArsI GACNNNNNNTTYG 2 cut(s) 1331, 1363
AspLEI GCGC 1 cut(s) 985
AspS9I GGNCC 1 cut(s) 2060
AsuC2I CCSGG 1 cut(s) 2059
AsuHPI GGTGA 8 cut(s) 920, 1097, 1466, 1533, 1658, 1805, 2000, 2129
BaeI ACNNNNGTAYC 2 cut(s) 185, 218
BarI GAAGNNNNNNTAC 2 cut(s) 1906, 1938
Bbv12I GWGCWC 1 cut(s) 1436
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 7 cut(s) 265, 338, 342, 1218, 1221, 2105, 2155
BccI CCATC 7 cut(s) 2, 1076, 1456, 1637, 1882, 1983, 2071
BceAI ACGGC 3 cut(s) 282, 696, 1769
BcgI CGANNNNNNTGC 4 cut(s) 986, 1020, 2150, 2184
BciT130I CCWGG 3 cut(s) 419, 969, 1374
BclI TGATCA 3 cut(s) 138, 1486, 1798
BcnI CCSGG 1 cut(s) 2059
BcoDI GTCTC 7 cut(s) 218, 538, 563, 749, 1375, 1690, 2040
BfaI CTAG 4 cut(s) 807, 876, 1053, 1203
BfmI CTRYAG 2 cut(s) 603, 915
BfrI CTTAAG 1 cut(s) 1196
BfuAI ACCTGC 2 cut(s) 1700, 2080
BisI GCNGC 8 cut(s) 22, 254, 327, 356, 1207, 1210, 2094, 2169
BlpI GCTNAGC 1 cut(s) 2127
BlsI GCNGC 8 cut(s) 23, 255, 328, 357, 1208, 1211, 2095, 2170
Bme1390I CCNGG 4 cut(s) 419, 969, 1374, 2059
BmgT120I GGNCC 1 cut(s) 2060
BmrFI CCNGG 4 cut(s) 419, 969, 1374, 2059
BmsI GCATC 4 cut(s) 698, 970, 1751, 1893
BpmI CTGGAG 1 cut(s) 229
Bpu10I CCTNAGC 2 cut(s) 351, 684
Bpu1102I GCTNAGC 1 cut(s) 2127
BpuEI CTTGAG 2 cut(s) 1306, 1813
BpuMI CCSGG 1 cut(s) 2059
Bsa29I ATCGAT 1 cut(s) 75
BsaHI GRCGYC 1 cut(s) 275
BsaI GGTCTC 3 cut(s) 563, 749, 2040
BsaJI CCNNGG 2 cut(s) 22, 36
BsaWI WCCGGW 1 cut(s) 84
Bsc4I CCNNNNNNNGG 3 cut(s) 1014, 1670, 1819
Bse118I RCCGGY 1 cut(s) 2020
Bse1I ACTGG 1 cut(s) 2118
BseAI TCCGGA 1 cut(s) 84
BseBI CCWGG 3 cut(s) 419, 969, 1374
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 22, 36
BseGI GGATG 3 cut(s) 440, 1660, 1867
BseLI CCNNNNNNNGG 3 cut(s) 1014, 1670, 1819
BseMII CTCAG 4 cut(s) 342, 675, 760, 1023
BseNI ACTGG 1 cut(s) 2118
BseRI GAGGAG 3 cut(s) 301, 1529, 1892
BseXI GCAGC 7 cut(s) 265, 338, 342, 1218, 1221, 2105, 2155
BsgI GTGCAG 1 cut(s) 2112
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 6 cut(s) 21, 620, 833, 1839, 2057, 2062
BshVI ATCGAT 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 1436
BsiSI CCGG 4 cut(s) 85, 278, 2021, 2058
BslI CCNNNNNNNGG 3 cut(s) 1014, 1670, 1819
BsmAI GTCTC 7 cut(s) 218, 538, 563, 749, 1375, 1690, 2040
BsmBI CGTCTC 1 cut(s) 1690
BsmI GAATGC 2 cut(s) 103, 856
BsnI GGCC 6 cut(s) 21, 620, 833, 1839, 2057, 2062
Bso31I GGTCTC 3 cut(s) 563, 749, 2040
Bsp1286I GDGCHC 1 cut(s) 1436
Bsp13I TCCGGA 1 cut(s) 84
Bsp1720I GCTNAGC 1 cut(s) 2127
BspACI CCGC 4 cut(s) 22, 24, 1358, 2043
BspANI GGCC 6 cut(s) 21, 620, 833, 1839, 2057, 2062
BspCNI CTCAG 4 cut(s) 343, 676, 759, 1022
BspDI ATCGAT 1 cut(s) 75
BspEI TCCGGA 1 cut(s) 84
BspFNI CGCG 1 cut(s) 24
BspHI TCATGA 2 cut(s) 151, 1717
BspMI ACCTGC 2 cut(s) 1700, 2080
BspPI GGATC 3 cut(s) 80, 698, 1439
BspQI GCTCTTC 1 cut(s) 1281
BspTI CTTAAG 1 cut(s) 1196
BspTNI GGTCTC 3 cut(s) 563, 749, 2040
BsrBI CCGCTC 1 cut(s) 1358
BsrFI RCCGGY 1 cut(s) 2020
BsrI ACTGG 1 cut(s) 2118
BssAI RCCGGY 1 cut(s) 2020
BssECI CCNNGG 2 cut(s) 22, 36
BssNAI GTATAC 1 cut(s) 247
BssNI GRCGYC 1 cut(s) 275
BssT1I CCWWGG 1 cut(s) 36
Bst1107I GTATAC 1 cut(s) 247
Bst2UI CCWGG 3 cut(s) 419, 969, 1374
Bst4CI ACNGT 5 cut(s) 149, 1874, 1913, 2034, 2052
Bst6I CTCTTC 6 cut(s) 113, 527, 630, 694, 1281, 1995
BstACI GRCGYC 1 cut(s) 275
BstAFI CTTAAG 1 cut(s) 1196
BstC8I GCNNGC 2 cut(s) 1293, 2022
BstDSI CCRYGG 1 cut(s) 22
BstENI CCTNNNNNAGG 1 cut(s) 1012
BstF5I GGATG 3 cut(s) 440, 1660, 1867
BstFNI CGCG 1 cut(s) 24
BstHHI GCGC 1 cut(s) 985
BstMAI GTCTC 7 cut(s) 218, 538, 563, 749, 1375, 1690, 2040
BstMWI GCNNNNNNNGC 7 cut(s) 274, 355, 862, 1039, 1499, 2093, 2177
BstNI CCWGG 3 cut(s) 419, 969, 1374
BstNSI RCATGY 1 cut(s) 1295
BstSCI CCNGG 4 cut(s) 417, 967, 1372, 2057
BstSFI CTRYAG 2 cut(s) 603, 915
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 7 cut(s) 265, 338, 342, 1218, 1221, 2105, 2155
BstX2I RGATCY 2 cut(s) 703, 1444
BstYI RGATCY 2 cut(s) 703, 1444
BstZ17I GTATAC 1 cut(s) 247
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 6 cut(s) 21, 620, 833, 1839, 2057, 2062
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 1 cut(s) 22
BtsCI GGATG 3 cut(s) 440, 1660, 1867
BtsIMutI CAGTG 3 cut(s) 47, 1879, 2125
BveI ACCTGC 2 cut(s) 1700, 2080
Cac8I GCNNGC 2 cut(s) 1293, 2022
CaiI CAGNNNCTG 1 cut(s) 332
CciI TCATGA 2 cut(s) 151, 1717
CfoI GCGC 1 cut(s) 985
Cfr10I RCCGGY 1 cut(s) 2020
Cfr13I GGNCC 1 cut(s) 2060
Cfr42I CCGCGG 1 cut(s) 25
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 1 cut(s) 283
CsiI ACCWGGT 1 cut(s) 967
Csp6I GTAC 3 cut(s) 1267, 1317, 1971
CviAII CATG 9 cut(s) 152, 265, 616, 1112, 1274, 1292, 1490, 1601, 1718
CviQI GTAC 3 cut(s) 1267, 1317, 1971
DraI TTTAAA 1 cut(s) 1243
EaeI YGGCCR 1 cut(s) 2055
Eam1104I CTCTTC 6 cut(s) 113, 527, 630, 694, 1281, 1995
EarI CTCTTC 6 cut(s) 113, 527, 630, 694, 1281, 1995
Eco130I CCWWGG 1 cut(s) 36
Eco147I AGGCCT 1 cut(s) 1839
Eco31I GGTCTC 3 cut(s) 563, 749, 2040
Eco57I CTGAAG 3 cut(s) 137, 1170, 1632
EcoNI CCTNNNNNAGG 1 cut(s) 1012
EcoRII CCWGG 3 cut(s) 417, 967, 1372
EcoT14I CCWWGG 1 cut(s) 36
EcoT22I ATGCAT 2 cut(s) 1744, 1908
ErhI CCWWGG 1 cut(s) 36
Esp3I CGTCTC 1 cut(s) 1690
FaeI CATG 9 cut(s) 155, 268, 619, 1115, 1277, 1295, 1493, 1604, 1721
FalI AAGNNNNNCTT 2 cut(s) 691, 723
FatI CATG 9 cut(s) 151, 264, 615, 1111, 1273, 1291, 1489, 1600, 1717
FauI CCCGC 1 cut(s) 2050
FbaI TGATCA 3 cut(s) 138, 1486, 1798
FblI GTMKAC 2 cut(s) 246, 606
Fnu4HI GCNGC 8 cut(s) 22, 254, 327, 356, 1207, 1210, 2094, 2169
FokI GGATG 3 cut(s) 427, 1647, 1874
Fsp4HI GCNGC 8 cut(s) 22, 254, 327, 356, 1207, 1210, 2094, 2169
FspAI RTGCGCAY 1 cut(s) 984
FspBI CTAG 4 cut(s) 807, 876, 1053, 1203
FspI TGCGCA 1 cut(s) 984
GlaI GCGC 1 cut(s) 984
GluI GCNGC 8 cut(s) 22, 254, 327, 356, 1207, 1210, 2094, 2169
GsuI CTGGAG 1 cut(s) 229
HaeIII GGCC 6 cut(s) 21, 620, 833, 1839, 2057, 2062
HapII CCGG 4 cut(s) 85, 278, 2021, 2058
HgaI GACGC 1 cut(s) 283
HhaI GCGC 1 cut(s) 985
Hin1I GRCGYC 1 cut(s) 275
Hin1II CATG 9 cut(s) 155, 268, 619, 1115, 1277, 1295, 1493, 1604, 1721
Hin6I GCGC 1 cut(s) 983
HinP1I GCGC 1 cut(s) 983
HincII GTYRAC 2 cut(s) 415, 931
HindII GTYRAC 2 cut(s) 415, 931
HindIII AAGCTT 1 cut(s) 1614
HpaII CCGG 4 cut(s) 85, 278, 2021, 2058
HphI GGTGA 8 cut(s) 920, 1097, 1466, 1533, 1658, 1805, 2000, 2129
Hpy166II GTNNAC 5 cut(s) 247, 415, 607, 931, 2137
Hpy8I GTNNAC 5 cut(s) 247, 415, 607, 931, 2137
HpyCH4III ACNGT 5 cut(s) 149, 1874, 1913, 2034, 2052
HpyCH4IV ACGT 4 cut(s) 1122, 1969, 2113, 2133
HpyF10VI GCNNNNNNNGC 7 cut(s) 274, 355, 862, 1039, 1499, 2093, 2177
HpySE526I ACGT 4 cut(s) 1122, 1969, 2113, 2133
Hsp92I GRCGYC 1 cut(s) 275
Hsp92II CATG 9 cut(s) 155, 268, 619, 1115, 1277, 1295, 1493, 1604, 1721
HspAI GCGC 1 cut(s) 983
Kpn2I TCCGGA 1 cut(s) 84
KroI GCCGGC 1 cut(s) 2020
KroNI GCCGGC 1 cut(s) 2022
Ksp22I TGATCA 3 cut(s) 138, 1486, 1798
KspI CCGCGG 1 cut(s) 25
LguI GCTCTTC 1 cut(s) 1281
LmnI GCTCC 4 cut(s) 37, 421, 1516, 1706
Lsp1109I GCAGC 7 cut(s) 265, 338, 342, 1218, 1221, 2105, 2155
LweI GCATC 4 cut(s) 698, 970, 1751, 1893
MabI ACCWGGT 1 cut(s) 967
MaeI CTAG 4 cut(s) 807, 876, 1053, 1203
MaeII ACGT 4 cut(s) 1122, 1969, 2113, 2133
MaeIII GTNAC 4 cut(s) 908, 1085, 2028, 2145
MbiI CCGCTC 1 cut(s) 1358
MflI RGATCY 2 cut(s) 703, 1444
MhlI GDGCHC 1 cut(s) 1436
MluCI AATT 2 cut(s) 362, 1851
MlyI GAGTC 1 cut(s) 220
Mph1103I ATGCAT 2 cut(s) 1744, 1908
MroI TCCGGA 1 cut(s) 84
MroNI GCCGGC 1 cut(s) 2020
MslI CAYNNNNRTG 3 cut(s) 917, 1143, 1389
MspA1I CMGCKG 2 cut(s) 24, 2045
MspCI CTTAAG 1 cut(s) 1196
MspI CCGG 4 cut(s) 85, 278, 2021, 2058
MspR9I CCNGG 4 cut(s) 419, 969, 1374, 2059
Mva1269I GAATGC 2 cut(s) 103, 856
MvaI CCWGG 3 cut(s) 419, 969, 1374
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 7 cut(s) 274, 355, 862, 1039, 1499, 2093, 2177
NaeI GCCGGC 1 cut(s) 2022
NciI CCSGG 1 cut(s) 2059
NgoMIV GCCGGC 1 cut(s) 2020
NlaIII CATG 9 cut(s) 155, 268, 619, 1115, 1277, 1295, 1493, 1604, 1721
NmuCI GTSAC 3 cut(s) 908, 1085, 2028
NsbI TGCGCA 1 cut(s) 984
NsiI ATGCAT 2 cut(s) 1744, 1908
NspI RCATGY 1 cut(s) 1295
OliI CACNNNNGTG 1 cut(s) 917
PaeI GCATGC 1 cut(s) 1295
PagI TCATGA 2 cut(s) 151, 1717
PceI AGGCCT 1 cut(s) 1839
PciSI GCTCTTC 1 cut(s) 1281
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 2 cut(s) 103, 856
PdiI GCCGGC 1 cut(s) 2022
PfeI GAWTC 9 cut(s) 67, 395, 515, 940, 1361, 1784, 1955, 1991, 2011
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 1670
PkrI GCNGC 8 cut(s) 23, 255, 328, 357, 1208, 1211, 2095, 2170
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
Psp1406I AACGTT 1 cut(s) 2113
Psp6I CCWGG 3 cut(s) 417, 967, 1372
PspGI CCWGG 3 cut(s) 417, 967, 1372
PspPI GGNCC 1 cut(s) 2060
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 2 cut(s) 703, 1444
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 3 cut(s) 1268, 1318, 1972
RsaNI GTAC 3 cut(s) 1267, 1317, 1971
RseI CAYNNNNRTG 3 cut(s) 917, 1143, 1389
SacII CCGCGG 1 cut(s) 25
SapI GCTCTTC 1 cut(s) 1281
SatI GCNGC 8 cut(s) 22, 254, 327, 356, 1207, 1210, 2094, 2169
Sau96I GGNCC 1 cut(s) 2060
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 4 cut(s) 419, 969, 1374, 2059
SduI GDGCHC 1 cut(s) 1436
SexAI ACCWGGT 1 cut(s) 967
SfaNI GCATC 4 cut(s) 698, 970, 1751, 1893
SfcI CTRYAG 2 cut(s) 603, 915
Sfr303I CCGCGG 1 cut(s) 25
SgrBI CCGCGG 1 cut(s) 25
SmiMI CAYNNNNRTG 3 cut(s) 917, 1143, 1389
SmlI CTYRAG 3 cut(s) 1196, 1321, 1828
SmoI CTYRAG 3 cut(s) 1196, 1321, 1828
SphI GCATGC 1 cut(s) 1295
Sse9I AATT 2 cut(s) 362, 1851
SseBI AGGCCT 1 cut(s) 1839
SsiI CCGC 4 cut(s) 22, 24, 1358, 2043
SspMI CTAG 4 cut(s) 807, 876, 1053, 1203
StuI AGGCCT 1 cut(s) 1839
StyD4I CCNGG 4 cut(s) 417, 967, 1372, 2057
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 5 cut(s) 149, 1874, 1913, 2034, 2052
TaiI ACGT 4 cut(s) 1125, 1972, 2116, 2136
TaqI TCGA 6 cut(s) 75, 216, 225, 528, 964, 1379
TaqII GACCGA 2 cut(s) 935, 2016
TasI AATT 2 cut(s) 362, 1851
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 9 cut(s) 67, 395, 515, 940, 1361, 1784, 1955, 1991, 2011
TscAI CASTG 3 cut(s) 54, 1879, 2125
TseFI GTSAC 3 cut(s) 908, 1085, 2028
TseI GCWGC 7 cut(s) 253, 326, 355, 1206, 1209, 2093, 2168
Tsp45I GTSAC 3 cut(s) 908, 1085, 2028
TspGWI ACGGA 1 cut(s) 1557
TspRI CASTG 3 cut(s) 54, 1879, 2125
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 1670
Vha464I CTTAAG 1 cut(s) 1196
XagI CCTNNNNNAGG 1 cut(s) 1012
XbaI TCTAGA 2 cut(s) 806, 1052
XceI RCATGY 1 cut(s) 1295
XmiI GTMKAC 2 cut(s) 246, 606
XspI CTAG 4 cut(s) 807, 876, 1053, 1203
Zsp2I ATGCAT 2 cut(s) 1744, 1908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.