Rh2AG054000

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009820
Physical Location & Seq
Forward (+)
4289399 .. 4293589
4191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG054000.1

Sequence Viewer

Length: 1446 bp
ATGGCCTACTGTGTTGCTGTACTAACGCCTGTATCAGATATTCAGATAAAATACAAAGTCCTAGGATGTCAAAGATTGGTGAATTTCTTATTTGGGAAAAAAAGTATTGCTCTTTCGAATTCTAAGGCAGTTGCAATTTTGCAGGACAAGCCAAAAACTTATGGGTCTTTCCCTGTTACTGACGATAAAGGCAAATTGTCTAATGGGAATGGTGCGGTACTAAAAGGAGATAGGCATGACCATCTGTCAACAGAAAGGAGACTTGATCCTAATGGTGGCCACAAATTACAGAACAACAGGGAGGTTATAGCCTTGAAGAAGGATGAGCTCGATGATCAGACTAGGCATAGACCTTTAGGAAAAGAATACAAGTCTCTCAAAGGCCGGGAAGGTAGTCTCAAGGAAAAAGAGGGCCATGGCATCTTGTTCCAGGAAAGGCAAGAAGTTGCTCACAGGTATGAAGCAAGAGAGGATGCTCCTTCAAAACCAGTTAGATTAGGCAGTTCTTCTCGGGGAAAAGGAGCAGAAAGGCATTATAAAGCAGAGAATAGTGTTCGTAAAAGAGACGACCCCGACACTATTCCTCATGTAAAGCCAAATGTTGCCGGACCACCTGTGAAAAGTGATGGTAAAGATTCTTTTGCTGGTAAAGAAGAAGGAACACCTAAATTGAAGCCCTTCTCCAACTATGGCCTTCCTCCTCCTTATGTGAAATCTAATGTCAGAGCAAAAGATAGAAAACATGAAGCTAATTTCGGTCATGATTGTTCTCAAGGTACTGGCATCTCTATGGATCCTGCAGCATATAAGAGAGCCTTTGCTGATAATGTGTTGGAAAGGGTACAACTAGGTCGTGATCATGAGAGGCGTGCTACTGCAAAAGTTGATAGTTATGATCATGAGAAGGGGCACGTTCATCAGGATGATGATATAGCTACCAACAATGTACCAAAACCAAGATCATCTAGAAGGAGACCCTCAAGATCGCGCTCCTCTACCAATGATGCTGGCAACGGTGAAGATATAGGAGCTGCAATGAGCAAACCAAGAAGCAGGAGTAGGAGAAGGGATGAGTCAAGACGTGGCCTGCAGCTGTTGTTTGATGATGACCACCACCACAGGAAAGATGATGAAGAGAGAAGAATTGACGAACTGCTGCTTCATTACAGCACAAAGCCATCTAACTTGGAGCCAGGGCAGTCAAGAAGAAAGTCAAAAAGTCGTCATGCAAGCAGAGATAAGCCTGCTGGGGAGGAGTCTGAACCAACTCATGTAAGATCAACTTCCCTTCCTCATGAACATTCAGGTCCATCAGAGACGCCCAAAGTATTTGCTCGTGCGGCTTCCTTCCAACCAGATGGGTCCAACCCAGCTCGGCATGTGCATCCCAAGTTGCCCGACTATGAGGATTTGGCTGCCAAGTTTGCAGCCTTAAGAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

481

Amino Acids

53.82

Weight (kDa)

9.69

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
AccII CGCG 1 cut(s) 988
AciI CCGC 2 cut(s) 215, 1340
AclWI GGATC 3 cut(s) 260, 788, 801
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 2 cut(s) 82, 118
AcyI GRCGYC 1 cut(s) 1319
AfaI GTAC 5 cut(s) 21, 219, 778, 843, 948
AfiI CCNNNNNNNGG 1 cut(s) 275
AflII CTTAAG 1 cut(s) 1432
AgsI TTSAA 3 cut(s) 316, 483, 673
AhdI GACNNNNNGTC 1 cut(s) 244
AjiI CACGTC 1 cut(s) 1082
AjnI CCWGG 2 cut(s) 429, 1192
AluBI AGCT 6 cut(s) 328, 749, 935, 1031, 1093, 1373
AluI AGCT 6 cut(s) 328, 749, 935, 1031, 1093, 1373
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 6 cut(s) 253, 378, 401, 558, 967, 1310
AlwI GGATC 3 cut(s) 260, 788, 801
Ama87I CYCGRG 1 cut(s) 510
AoxI GGCC 6 cut(s) 3, 277, 382, 412, 691, 1084
ApeKI GCWGC 6 cut(s) 800, 1031, 1090, 1156, 1415, 1427
ApoI RAATTY 2 cut(s) 82, 118
Asp700I GAANNNNTTC 1 cut(s) 677
AspA2I CCTAGG 1 cut(s) 61
AspLEI GCGC 1 cut(s) 990
AspS9I GGNCC 4 cut(s) 412, 608, 1307, 1362
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 2 cut(s) 91, 1028
AsuII TTCGAA 1 cut(s) 116
AvaI CYCGRG 1 cut(s) 510
AvaII GGWCC 3 cut(s) 608, 1307, 1362
AvrII CCTAGG 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 912
BaeI ACNNNNGTAYC 2 cut(s) 15, 48
BalI TGGCCA 1 cut(s) 279
BamHI GGATCC 1 cut(s) 793
BanII GRGCYC 1 cut(s) 330
BauI CACGAG 1 cut(s) 1335
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 6 cut(s) 812, 1018, 1102, 1143, 1402, 1439
BccI CCATC 5 cut(s) 249, 620, 1186, 1318, 1352
BciT130I CCWGG 2 cut(s) 431, 1194
BclI TGATCA 3 cut(s) 334, 856, 895
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 6 cut(s) 253, 378, 401, 558, 967, 1310
BfaI CTAG 4 cut(s) 62, 342, 848, 966
BfmI CTRYAG 2 cut(s) 798, 1088
BfrI CTTAAG 1 cut(s) 1432
BisI GCNGC 7 cut(s) 801, 1032, 1091, 1157, 1341, 1416, 1428
BlnI CCTAGG 1 cut(s) 61
BlsI GCNGC 7 cut(s) 802, 1033, 1092, 1158, 1342, 1417, 1429
Bme1390I CCNGG 3 cut(s) 386, 431, 1194
Bme18I GGWCC 3 cut(s) 608, 1307, 1362
BmeRI GACNNNNNGTC 1 cut(s) 244
BmeT110I CYCGRG 1 cut(s) 510
BmgBI CACGTC 1 cut(s) 1082
BmgT120I GGNCC 4 cut(s) 412, 608, 1307, 1362
BmiI GGNNCC 3 cut(s) 795, 1191, 1363
BmrFI CCNGG 3 cut(s) 386, 431, 1194
BmsI GCATC 5 cut(s) 429, 463, 792, 994, 1393
Bpu14I TTCGAA 1 cut(s) 116
BpuEI CTTGAG 3 cut(s) 383, 756, 964
BpuMI CCSGG 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 1319
BsaI GGTCTC 1 cut(s) 967
BsaJI CCNNGG 3 cut(s) 61, 415, 1193
Bsc4I CCNNNNNNNGG 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 488, 784
Bse3DI GCAATG 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 431, 1194
BseDI CCNNGG 3 cut(s) 61, 415, 1193
BseGI GGATG 6 cut(s) 71, 328, 478, 928, 1075, 1384
BseLI CCNNNNNNNGG 1 cut(s) 275
BseMI GCAATG 1 cut(s) 1041
BseNI ACTGG 2 cut(s) 488, 784
BseRI GAGGAG 3 cut(s) 690, 982, 1268
BseSI GKGCMC 1 cut(s) 912
BseXI GCAGC 6 cut(s) 812, 1018, 1102, 1143, 1402, 1439
BseYI CCCAGC 2 cut(s) 1247, 1369
Bsh1236I CGCG 1 cut(s) 988
BshFI GGCC 6 cut(s) 5, 279, 384, 414, 693, 1086
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 510
BsiSI CCGG 2 cut(s) 385, 606
BslI CCNNNNNNNGG 1 cut(s) 275
BsmAI GTCTC 6 cut(s) 253, 378, 401, 558, 967, 1310
BsmBI CGTCTC 2 cut(s) 558, 1310
BsnI GGCC 6 cut(s) 5, 279, 384, 414, 693, 1086
Bso31I GGTCTC 1 cut(s) 967
BsoBI CYCGRG 1 cut(s) 510
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 330, 912
Bsp143I GATC 8 cut(s) 265, 334, 793, 856, 895, 959, 983, 1277
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 2 cut(s) 215, 1340
BspANI GGCC 6 cut(s) 5, 279, 384, 414, 693, 1086
BspFNI CGCG 1 cut(s) 988
BspHI TCATGA 4 cut(s) 760, 859, 898, 1294
BspLI GGNNCC 3 cut(s) 795, 1191, 1363
BspMAI CTGCAG 2 cut(s) 802, 1092
BspPI GGATC 3 cut(s) 260, 788, 801
BspT104I TTCGAA 1 cut(s) 116
BspTI CTTAAG 1 cut(s) 1432
BspTNI GGTCTC 1 cut(s) 967
BsrDI GCAATG 1 cut(s) 1041
BsrI ACTGG 2 cut(s) 488, 784
BssECI CCNNGG 3 cut(s) 61, 415, 1193
BssMI GATC 8 cut(s) 265, 334, 793, 856, 895, 959, 983, 1277
BssNI GRCGYC 1 cut(s) 1319
BssSI CACGAG 1 cut(s) 1335
BssT1I CCWWGG 2 cut(s) 61, 415
Bst2BI CACGAG 1 cut(s) 1335
Bst2UI CCWGG 2 cut(s) 431, 1194
Bst4CI ACNGT 2 cut(s) 11, 1016
Bst6I CTCTTC 1 cut(s) 1128
BstACI GRCGYC 1 cut(s) 1319
BstAFI CTTAAG 1 cut(s) 1432
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 5 cut(s) 870, 1009, 1088, 1231, 1245
BstDEI CTNAG 1 cut(s) 123
BstDSI CCRYGG 1 cut(s) 415
BstF5I GGATG 6 cut(s) 71, 328, 478, 928, 1075, 1384
BstFNI CGCG 1 cut(s) 988
BstHHI GCGC 1 cut(s) 990
BstKTI GATC 8 cut(s) 268, 337, 796, 859, 898, 962, 986, 1280
BstMAI GTCTC 6 cut(s) 253, 378, 401, 558, 967, 1310
BstMBI GATC 8 cut(s) 265, 334, 793, 856, 895, 959, 983, 1277
BstMWI GCNNNNNNNGC 3 cut(s) 148, 1340, 1424
BstNI CCWGG 2 cut(s) 431, 1194
BstNSI RCATGY 1 cut(s) 1382
BstSCI CCNGG 3 cut(s) 384, 429, 1192
BstSFI CTRYAG 2 cut(s) 798, 1088
BstSLI GKGCMC 1 cut(s) 912
BstUI CGCG 1 cut(s) 988
BstV1I GCAGC 6 cut(s) 812, 1018, 1102, 1143, 1402, 1439
BstX2I RGATCY 1 cut(s) 793
BstXI CCANNNNNNTGG 1 cut(s) 1358
BstYI RGATCY 1 cut(s) 793
BsuRI GGCC 6 cut(s) 5, 279, 384, 414, 693, 1086
BtgI CCRYGG 1 cut(s) 415
BtrI CACGTC 1 cut(s) 1082
BtsCI GGATG 6 cut(s) 71, 328, 478, 928, 1075, 1384
Cac8I GCNNGC 5 cut(s) 870, 1009, 1088, 1231, 1245
CciI TCATGA 4 cut(s) 760, 859, 898, 1294
CfoI GCGC 1 cut(s) 990
Cfr13I GGNCC 4 cut(s) 412, 608, 1307, 1362
CseI GACGC 1 cut(s) 1327
Csp6I GTAC 5 cut(s) 20, 218, 777, 842, 947
CviQI GTAC 5 cut(s) 20, 218, 777, 842, 947
DdeI CTNAG 1 cut(s) 123
DpnI GATC 8 cut(s) 267, 336, 795, 858, 897, 961, 985, 1279
DpnII GATC 8 cut(s) 265, 334, 793, 856, 895, 959, 983, 1277
DriI GACNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 1128
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 1 cut(s) 1128
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 2 cut(s) 61, 415
Eco24I GRGCYC 1 cut(s) 330
Eco31I GGTCTC 1 cut(s) 967
Eco47I GGWCC 3 cut(s) 608, 1307, 1362
Eco53kI GAGCTC 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 328
EcoRI GAATTC 1 cut(s) 118
EcoRII CCWGG 2 cut(s) 429, 1192
EcoT14I CCWWGG 2 cut(s) 61, 415
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 2 cut(s) 61, 415
Esp3I CGTCTC 2 cut(s) 558, 1310
FalI AAGNNNNNCTT 4 cut(s) 800, 832, 1267, 1299
FbaI TGATCA 3 cut(s) 334, 856, 895
Fnu4HI GCNGC 7 cut(s) 801, 1032, 1091, 1157, 1341, 1416, 1428
FokI GGATG 6 cut(s) 78, 335, 485, 935, 1082, 1371
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 7 cut(s) 801, 1032, 1091, 1157, 1341, 1416, 1428
FspBI CTAG 4 cut(s) 62, 342, 848, 966
GlaI GCGC 1 cut(s) 989
GluI GCNGC 7 cut(s) 801, 1032, 1091, 1157, 1341, 1416, 1428
GsaI CCCAGC 2 cut(s) 1251, 1373
HaeIII GGCC 6 cut(s) 5, 279, 384, 414, 693, 1086
HapII CCGG 2 cut(s) 385, 606
HgaI GACGC 1 cut(s) 1327
HhaI GCGC 1 cut(s) 990
Hin1I GRCGYC 1 cut(s) 1319
Hin6I GCGC 1 cut(s) 988
HinP1I GCGC 1 cut(s) 988
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HinfI GANTC 3 cut(s) 635, 1073, 1256
HpaII CCGG 2 cut(s) 385, 606
HphI GGTGA 2 cut(s) 91, 1028
Hpy166II GTNNAC 1 cut(s) 249
Hpy188I TCNGA 6 cut(s) 37, 45, 339, 725, 1261, 1315
Hpy8I GTNNAC 1 cut(s) 249
HpyCH4III ACNGT 2 cut(s) 11, 1016
HpyCH4IV ACGT 2 cut(s) 912, 1081
HpyCH4V TGCA 9 cut(s) 134, 142, 800, 878, 1034, 1090, 1229, 1384, 1427
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 1340, 1424
HpyF3I CTNAG 1 cut(s) 123
HpySE526I ACGT 2 cut(s) 912, 1081
Hsp92I GRCGYC 1 cut(s) 1319
HspAI GCGC 1 cut(s) 988
Ksp22I TGATCA 3 cut(s) 334, 856, 895
Kzo9I GATC 8 cut(s) 265, 334, 793, 856, 895, 959, 983, 1277
LmnI GCTCC 5 cut(s) 481, 521, 995, 1028, 1189
Lsp1109I GCAGC 6 cut(s) 812, 1018, 1102, 1143, 1402, 1439
LweI GCATC 5 cut(s) 429, 463, 792, 994, 1393
MaeI CTAG 4 cut(s) 62, 342, 848, 966
MaeII ACGT 2 cut(s) 912, 1081
MaeIII GTNAC 1 cut(s) 175
MalI GATC 8 cut(s) 267, 336, 795, 858, 897, 961, 985, 1279
MboI GATC 8 cut(s) 265, 334, 793, 856, 895, 959, 983, 1277
MboII GAAGA 7 cut(s) 328, 498, 665, 1031, 1145, 1152, 1218
MflI RGATCY 1 cut(s) 793
MhlI GDGCHC 2 cut(s) 330, 912
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 8 cut(s) 82, 118, 135, 194, 284, 668, 751, 1143
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 2 cut(s) 1082, 1265
MmeI TCCRAC 4 cut(s) 708, 813, 1375, 1389
Mox20I TGGCCA 1 cut(s) 279
MroXI GAANNNNTTC 1 cut(s) 677
MscI TGGCCA 1 cut(s) 279
MseI TTAA 1 cut(s) 1433
MslI CAYNNNNRTG 3 cut(s) 456, 788, 921
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 1093
MspCI CTTAAG 1 cut(s) 1432
MspI CCGG 2 cut(s) 385, 606
MspR9I CCNGG 3 cut(s) 386, 431, 1194
MvaI CCWGG 2 cut(s) 431, 1194
MvnI CGCG 1 cut(s) 988
MwoI GCNNNNNNNGC 3 cut(s) 148, 1340, 1424
NciI CCSGG 1 cut(s) 386
NcoI CCATGG 1 cut(s) 415
NdeII GATC 8 cut(s) 265, 334, 793, 856, 895, 959, 983, 1277
NlaIV GGNNCC 3 cut(s) 795, 1191, 1363
NmeAIII GCCGAG 1 cut(s) 1354
NspI RCATGY 1 cut(s) 1382
NspV TTCGAA 1 cut(s) 116
PagI TCATGA 4 cut(s) 760, 859, 898, 1294
PdmI GAANNNNTTC 1 cut(s) 677
PfeI GAWTC 1 cut(s) 635
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 7 cut(s) 802, 1033, 1092, 1158, 1342, 1417, 1429
PleI GAGTC 2 cut(s) 1081, 1264
PpsI GAGTC 2 cut(s) 1081, 1264
PsiI TTATAA 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 429, 1192
PspFI CCCAGC 2 cut(s) 1247, 1369
PspGI CCWGG 2 cut(s) 429, 1192
PspN4I GGNNCC 3 cut(s) 795, 1191, 1363
PspPI GGNCC 4 cut(s) 412, 608, 1307, 1362
PstI CTGCAG 2 cut(s) 802, 1092
PsuI RGATCY 1 cut(s) 793
PvuII CAGCTG 1 cut(s) 1093
RsaI GTAC 5 cut(s) 21, 219, 778, 843, 948
RsaNI GTAC 5 cut(s) 20, 218, 777, 842, 947
RseI CAYNNNNRTG 3 cut(s) 456, 788, 921
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 1 cut(s) 1433
SatI GCNGC 7 cut(s) 801, 1032, 1091, 1157, 1341, 1416, 1428
Sau3AI GATC 8 cut(s) 265, 334, 793, 856, 895, 959, 983, 1277
Sau96I GGNCC 4 cut(s) 412, 608, 1307, 1362
SchI GAGTC 2 cut(s) 1082, 1265
ScrFI CCNGG 3 cut(s) 386, 431, 1194
SduI GDGCHC 2 cut(s) 330, 912
SfaNI GCATC 5 cut(s) 429, 463, 792, 994, 1393
SfcI CTRYAG 2 cut(s) 798, 1088
SfuI TTCGAA 1 cut(s) 116
SinI GGWCC 3 cut(s) 608, 1307, 1362
SmiMI CAYNNNNRTG 3 cut(s) 456, 788, 921
SmlI CTYRAG 4 cut(s) 398, 771, 979, 1432
SmoI CTYRAG 4 cut(s) 398, 771, 979, 1432
Sse9I AATT 8 cut(s) 82, 118, 135, 194, 284, 668, 751, 1143
SsiI CCGC 2 cut(s) 215, 1340
SspMI CTAG 4 cut(s) 62, 342, 848, 966
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 3 cut(s) 384, 429, 1192
StyI CCWWGG 2 cut(s) 61, 415
TaaI ACNGT 2 cut(s) 11, 1016
TaiI ACGT 2 cut(s) 915, 1084
TaqI TCGA 2 cut(s) 116, 330
TaqII GACCGA 1 cut(s) 746
TasI AATT 8 cut(s) 82, 118, 135, 194, 284, 668, 751, 1143
TatI WGTACW 1 cut(s) 19
TauI GCSGC 1 cut(s) 1343
TfiI GAWTC 1 cut(s) 635
Tru1I TTAA 1 cut(s) 1433
Tru9I TTAA 1 cut(s) 1433
TseI GCWGC 6 cut(s) 800, 1031, 1090, 1156, 1415, 1427
TspDTI ATGAA 6 cut(s) 474, 759, 905, 1146, 1151, 1311
Vha464I CTTAAG 1 cut(s) 1432
VpaK11BI GGWCC 3 cut(s) 608, 1307, 1362
XapI RAATTY 2 cut(s) 82, 118
XbaI TCTAGA 1 cut(s) 965
XceI RCATGY 1 cut(s) 1382
XmaJI CCTAGG 1 cut(s) 61
XmnI GAANNNNTTC 1 cut(s) 677
XspI CTAG 4 cut(s) 62, 342, 848, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.