RLG00000016119

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009820
Physical Location & Seq
Forward (+)
4033072 .. 4039356
6285 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016119

Sequence Viewer

Length: 1791 bp
ATGTCTCTTAGCCCATTGATGTTCAGGTCACTGGTGAGGCTACTAACATCACTGCTGAGTCGAATCGTTCCGGTCACCGAGTCGGAGAAAATGCTGGACGGACTGCTCGGCCGTGGATTCGGCGCCAAGTGTAAATCGCTGATAAAATTGACGAAAACTCGGATCGATGTGATTCGGAGGAAGAGGAACGCGACGCAGAAGTTTTTGAAGAAAGATATAGCTGATCTACTCAACCATGGCCTCGAAATTAATGCTTTCGGAAGGGCTGAGGGACTTACAGTGGAGGTGATACTTTCGGCGTGTTACGATTTCGTTGAGCGATGCTGTGATTTGGTGTTGAAGCATCTTTCATTTGTTGAGAACATAGCTTCAAAGCCTCCCACATTGGAAAAGAAGGTTCAATTGATGAAAGACATAGCATTAGAGTATTCAATAAAGTGGGACTCCAAGGCCTTTGAACAAATGATGTCTAAACCTCCTGCTTTTATACAGGACAAGCCAAAAACTTATGGGTCTTTCCCTGTTACTGATGATAAAGGCAAATTGTCTAATGGGAATGGTGCAGTACTAAAAGGAGATAGGCATGACCATCTGTCAACAGAAAGGAGACTTGATCCTAATGGTGGCCATAAATTACAGAACAACAAGGAGGTTTTAGCCTTGAAGAAGGATGAGCTCAATGATCAGACTAGGCATAGACATTTAGGAAAAGAATACAAGTCTCTCAAAGGCCAGGAAGGTAGTCTCAAGGAAAAAGAGGGCCATGGCATCTTGTTCCAGGAAAGGCAAGAAGTTGCTTACAGGTATGAAGCAAGAGAGGATGCTCCTTCAAAACCAGTTAGATTAGGCAGTTCTTCTCAGGGAAAAGGAGCAGAAAGGCATTATGAAGCGGAGAATAGTGTTCGTAAAAGAGACAAGCCCGACACTATTCCTCATGTAAAGCCAAATGTTGCTGGACCACCTGTGAAAAGTGATGGTAAAGATTCTTTTGCTGGTAAAGAAGAAGGAACACCTAAATTGAAGCCCTTCTCCAACTATGGCCTTCCTCCTCCTTATGTGAAATCTAATGTCAGAGCAAAAGATAGAAAACATGAAGCTAATTTGGGTCATGATTGTTCTCAAGGTACCGGCATCTCTATGGATCCTGCAGCATATAAGACAGCCTTTGCTGATAATGTGTTGGAAAGAGTACAACTAGGTTGTGATCATGAGAGGCGTGCTACTGCAAAAGTTGATAGTTATGATCATGGGAAGGAGCACGTTCATCAGGATGATATAGCTACCAACAATGTACCAAAACCAAGATCATCTAGAAGGAGACCCTCAAGATCGCGCTCCTCTACCAGTGATGCTGGCAACGGTGAAGATATAGGAGTTGCAATGAGCAAACCAAGAAGCAGGAGTAGGAGAAGGGATGAGTCAAGACGCGGCCTGCAGCTCTTGTTTGATGATGACCACCACCACAGGAAAGATGACGAAGAGAGAAGAATTGACGAACTGCTGCTTCATTACAGCACAAAGCCATCTAACTTGGAGCCAGGGCAGTCAAGAAGAAAGTCAAAAAGTCGTCATGGAAGCAGAGATAAGCCTGCTGGGGAGGAGTCTGAACCAACTCATGTAAGATCAACTTCCCTTCCTCATGAACATTCAGGTCCATCAGAGTCGCCCAAAGTATTTGCTCGTGCGGCTTCCTTCCAACCAGATGGGTCCAACCCAGCTCGGCACGTGCATCCCAAGTTGCCCGACTATGAGGATTTGGCTGCCAAGTTTGCAGCCTTAAGAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

597

Amino Acids

66.88

Weight (kDa)

9.54

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 45 - 131 9.5e-18 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1124
AccB1I GGYRCC 2 cut(s) 122, 1124
AccII CGCG 3 cut(s) 191, 1333, 1428
AciI CCGC 3 cut(s) 890, 1428, 1685
AclWI GGATC 4 cut(s) 170, 608, 1136, 1149
AcoI YGGCCR 2 cut(s) 109, 625
AcvI CACGTG 1 cut(s) 1726
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 4 cut(s) 567, 1126, 1191, 1293
AfiI CCNNNNNNNGG 1 cut(s) 623
AflII CTTAAG 1 cut(s) 1777
AgsI TTSAA 9 cut(s) 208, 340, 372, 401, 432, 458, 664, 831, 1021
AhdI GACNNNNNGTC 1 cut(s) 592
AjnI CCWGG 3 cut(s) 732, 777, 1537
AluBI AGCT 7 cut(s) 221, 368, 676, 1097, 1280, 1438, 1718
AluI AGCT 7 cut(s) 221, 368, 676, 1097, 1280, 1438, 1718
Alw21I GWGCWC 2 cut(s) 678, 1260
Alw26I GTCTC 6 cut(s) 9, 601, 726, 749, 906, 1312
AlwI GGATC 4 cut(s) 170, 608, 1136, 1149
AoxI GGCC 8 cut(s) 109, 238, 450, 625, 730, 760, 1039, 1429
ApeKI GCWGC 5 cut(s) 1148, 1435, 1501, 1760, 1772
AseI ATTAAT 1 cut(s) 249
Asp700I GAANNNNTTC 1 cut(s) 1025
Asp718I GGTACC 1 cut(s) 1124
AspLEI GCGC 2 cut(s) 125, 1335
AspS9I GGNCC 4 cut(s) 760, 956, 1652, 1707
AsuHPI GGTGA 4 cut(s) 46, 67, 298, 1373
AvaII GGWCC 3 cut(s) 956, 1652, 1707
BalI TGGCCA 1 cut(s) 627
BamHI GGATCC 1 cut(s) 1141
BanI GGYRCC 2 cut(s) 122, 1124
BanII GRGCYC 1 cut(s) 678
BauI CACGAG 1 cut(s) 1680
BbrPI CACGTG 1 cut(s) 1726
Bbv12I GWGCWC 2 cut(s) 678, 1260
BbvCI CCTCAGC 1 cut(s) 267
BbvI GCAGC 5 cut(s) 1160, 1447, 1488, 1747, 1784
BccI CCATC 5 cut(s) 597, 968, 1531, 1663, 1697
BceAI ACGGC 1 cut(s) 96
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BciT130I CCWGG 3 cut(s) 734, 779, 1539
BclI TGATCA 3 cut(s) 682, 1204, 1243
BcoDI GTCTC 6 cut(s) 9, 601, 726, 749, 906, 1312
BfaI CTAG 3 cut(s) 690, 1196, 1311
BfmI CTRYAG 2 cut(s) 1146, 1433
BfoI RGCGCY 1 cut(s) 126
BfrI CTTAAG 1 cut(s) 1777
BisI GCNGC 7 cut(s) 1149, 1429, 1436, 1502, 1686, 1761, 1773
BlsI GCNGC 7 cut(s) 1150, 1430, 1437, 1503, 1687, 1762, 1774
BmcAI AGTACT 1 cut(s) 567
Bme1390I CCNGG 3 cut(s) 734, 779, 1539
Bme18I GGWCC 3 cut(s) 956, 1652, 1707
BmeRI GACNNNNNGTC 1 cut(s) 592
BmgT120I GGNCC 4 cut(s) 760, 956, 1652, 1707
BmiI GGNNCC 5 cut(s) 124, 1126, 1143, 1536, 1708
BmrFI CCNGG 3 cut(s) 734, 779, 1539
BmsI GCATC 7 cut(s) 311, 352, 777, 811, 1140, 1339, 1738
Bpu10I CCTNAGC 1 cut(s) 267
BpuEI CTTGAG 3 cut(s) 731, 1104, 1309
Bsa29I ATCGAT 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 1726
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 1312
BsaJI CCNNGG 5 cut(s) 112, 235, 447, 763, 1538
BsaWI WCCGGW 1 cut(s) 70
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse118I RCCGGY 1 cut(s) 1127
Bse1I ACTGG 3 cut(s) 36, 836, 1344
Bse3DI GCAATG 1 cut(s) 1386
BseBI CCWGG 3 cut(s) 734, 779, 1539
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 5 cut(s) 112, 235, 447, 763, 1538
BseGI GGATG 5 cut(s) 676, 826, 1276, 1420, 1729
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMI GCAATG 1 cut(s) 1386
BseMII CTCAG 3 cut(s) 47, 258, 872
BseNI ACTGG 3 cut(s) 36, 836, 1344
BseRI GAGGAG 3 cut(s) 1038, 1327, 1613
BseX3I CGGCCG 1 cut(s) 109
BseXI GCAGC 5 cut(s) 1160, 1447, 1488, 1747, 1784
BseYI CCCAGC 2 cut(s) 1592, 1714
BsgI GTGCAG 1 cut(s) 582
Bsh1236I CGCG 3 cut(s) 191, 1333, 1428
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 8 cut(s) 111, 240, 452, 627, 732, 762, 1041, 1431
BshNI GGYRCC 2 cut(s) 122, 1124
BshVI ATCGAT 1 cut(s) 165
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 2 cut(s) 678, 1260
BsiSI CCGG 2 cut(s) 71, 1128
BslFI GGGAC 2 cut(s) 285, 455
BslI CCNNNNNNNGG 1 cut(s) 623
BsmAI GTCTC 6 cut(s) 9, 601, 726, 749, 906, 1312
BsmFI GGGAC 2 cut(s) 285, 455
BsnI GGCC 8 cut(s) 111, 240, 452, 627, 732, 762, 1041, 1431
Bso31I GGTCTC 1 cut(s) 1312
Bsp1286I GDGCHC 2 cut(s) 678, 1260
Bsp19I CCATGG 2 cut(s) 235, 763
BspACI CCGC 3 cut(s) 890, 1428, 1685
BspANI GGCC 8 cut(s) 111, 240, 452, 627, 732, 762, 1041, 1431
BspCNI CTCAG 3 cut(s) 48, 259, 871
BspDI ATCGAT 1 cut(s) 165
BspFNI CGCG 3 cut(s) 191, 1333, 1428
BspHI TCATGA 3 cut(s) 1108, 1207, 1639
BspLI GGNNCC 5 cut(s) 124, 1126, 1143, 1536, 1708
BspMAI CTGCAG 2 cut(s) 1150, 1437
BspPI GGATC 4 cut(s) 170, 608, 1136, 1149
BspT107I GGYRCC 2 cut(s) 122, 1124
BspTI CTTAAG 1 cut(s) 1777
BspTNI GGTCTC 1 cut(s) 1312
BsrDI GCAATG 1 cut(s) 1386
BsrFI RCCGGY 1 cut(s) 1127
BsrI ACTGG 3 cut(s) 36, 836, 1344
BssAI RCCGGY 1 cut(s) 1127
BssECI CCNNGG 5 cut(s) 112, 235, 447, 763, 1538
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 1680
BssT1I CCWWGG 3 cut(s) 235, 447, 763
Bst2BI CACGAG 1 cut(s) 1680
Bst2UI CCWGG 3 cut(s) 734, 779, 1539
Bst4CI ACNGT 2 cut(s) 280, 1361
Bst6I CTCTTC 2 cut(s) 176, 1473
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 1777
BstBAI YACGTR 1 cut(s) 1726
BstC8I GCNNGC 4 cut(s) 1218, 1354, 1433, 1590
BstDEI CTNAG 4 cut(s) 8, 56, 267, 858
BstDSI CCRYGG 3 cut(s) 112, 235, 763
BstEII GGTNACC 1 cut(s) 73
BstF5I GGATG 5 cut(s) 676, 826, 1276, 1420, 1729
BstFNI CGCG 3 cut(s) 191, 1333, 1428
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 2 cut(s) 125, 1335
BstMAI GTCTC 6 cut(s) 9, 601, 726, 749, 906, 1312
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 2 cut(s) 1685, 1769
BstNI CCWGG 3 cut(s) 734, 779, 1539
BstPI GGTNACC 1 cut(s) 73
BstSCI CCNGG 3 cut(s) 732, 777, 1537
BstSFI CTRYAG 2 cut(s) 1146, 1433
BstUI CGCG 3 cut(s) 191, 1333, 1428
BstV1I GCAGC 5 cut(s) 1160, 1447, 1488, 1747, 1784
BstX2I RGATCY 1 cut(s) 1141
BstXI CCANNNNNNTGG 1 cut(s) 1703
BstYI RGATCY 1 cut(s) 1141
BstZI CGGCCG 1 cut(s) 109
Bsu15I ATCGAT 1 cut(s) 165
BsuRI GGCC 8 cut(s) 111, 240, 452, 627, 732, 762, 1041, 1431
BsuTUI ATCGAT 1 cut(s) 165
BtgI CCRYGG 3 cut(s) 112, 235, 763
BtgZI GCGATG 1 cut(s) 334
BtsCI GGATG 5 cut(s) 676, 826, 1276, 1420, 1729
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 4 cut(s) 29, 50, 285, 1351
Cac8I GCNNGC 4 cut(s) 1218, 1354, 1433, 1590
CciI TCATGA 3 cut(s) 1108, 1207, 1639
CfoI GCGC 2 cut(s) 125, 1335
Cfr10I RCCGGY 1 cut(s) 1127
Cfr13I GGNCC 4 cut(s) 760, 956, 1652, 1707
ClaI ATCGAT 1 cut(s) 165
CseI GACGC 2 cut(s) 202, 1434
Csp6I GTAC 4 cut(s) 566, 1125, 1190, 1292
CviQI GTAC 4 cut(s) 566, 1125, 1190, 1292
DdeI CTNAG 4 cut(s) 8, 56, 267, 858
DinI GGCGCC 1 cut(s) 124
DriI GACNNNNNGTC 1 cut(s) 592
EaeI YGGCCR 2 cut(s) 109, 625
EagI CGGCCG 1 cut(s) 109
Eam1104I CTCTTC 2 cut(s) 176, 1473
Eam1105I GACNNNNNGTC 1 cut(s) 592
EarI CTCTTC 2 cut(s) 176, 1473
Ecl136II GAGCTC 1 cut(s) 676
EclXI CGGCCG 1 cut(s) 109
Eco130I CCWWGG 3 cut(s) 235, 447, 763
Eco147I AGGCCT 1 cut(s) 452
Eco24I GRGCYC 1 cut(s) 678
Eco31I GGTCTC 1 cut(s) 1312
Eco47I GGWCC 3 cut(s) 956, 1652, 1707
Eco52I CGGCCG 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 676
Eco72I CACGTG 1 cut(s) 1726
Eco91I GGTNACC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 676
EcoO65I GGTNACC 1 cut(s) 73
EcoRII CCWGG 3 cut(s) 732, 777, 1537
EcoT14I CCWWGG 3 cut(s) 235, 447, 763
EcoT38I GRGCYC 1 cut(s) 678
EgeI GGCGCC 1 cut(s) 124
EheI GGCGCC 1 cut(s) 124
ErhI CCWWGG 3 cut(s) 235, 447, 763
FalI AAGNNNNNCTT 4 cut(s) 1148, 1180, 1612, 1644
FaqI GGGAC 2 cut(s) 285, 455
FbaI TGATCA 3 cut(s) 682, 1204, 1243
Fnu4HI GCNGC 7 cut(s) 1149, 1429, 1436, 1502, 1686, 1761, 1773
FokI GGATG 5 cut(s) 683, 833, 1283, 1427, 1716
FriOI GRGCYC 1 cut(s) 678
Fsp4HI GCNGC 7 cut(s) 1149, 1429, 1436, 1502, 1686, 1761, 1773
FspBI CTAG 3 cut(s) 690, 1196, 1311
GlaI GCGC 2 cut(s) 124, 1334
GluI GCNGC 7 cut(s) 1149, 1429, 1436, 1502, 1686, 1761, 1773
GsaI CCCAGC 2 cut(s) 1596, 1718
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 8 cut(s) 111, 240, 452, 627, 732, 762, 1041, 1431
HapII CCGG 2 cut(s) 71, 1128
HgaI GACGC 2 cut(s) 202, 1434
HhaI GCGC 2 cut(s) 125, 1335
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 2 cut(s) 123, 1333
HinP1I GCGC 2 cut(s) 123, 1333
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HpaII CCGG 2 cut(s) 71, 1128
HphI GGTGA 4 cut(s) 46, 67, 298, 1373
Hpy166II GTNNAC 1 cut(s) 597
Hpy188I TCNGA 8 cut(s) 85, 162, 177, 260, 687, 1073, 1606, 1660
Hpy188III TCNNGA 8 cut(s) 1109, 1208, 1268, 1311, 1326, 1422, 1548, 1640
Hpy8I GTNNAC 1 cut(s) 597
Hpy99I CGWCG 1 cut(s) 196
HpyCH4III ACNGT 2 cut(s) 280, 1361
HpyCH4IV ACGT 2 cut(s) 1260, 1725
HpyCH4V TGCA 7 cut(s) 563, 1148, 1226, 1379, 1435, 1729, 1772
HpyF10VI GCNNNNNNNGC 2 cut(s) 1685, 1769
HpyF3I CTNAG 4 cut(s) 8, 56, 267, 858
HpySE526I ACGT 2 cut(s) 1260, 1725
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 2 cut(s) 123, 1333
KasI GGCGCC 1 cut(s) 122
KpnI GGTACC 1 cut(s) 1128
Ksp22I TGATCA 3 cut(s) 682, 1204, 1243
LmnI GCTCC 5 cut(s) 829, 869, 1255, 1340, 1534
Lsp1109I GCAGC 5 cut(s) 1160, 1447, 1488, 1747, 1784
LweI GCATC 7 cut(s) 311, 352, 777, 811, 1140, 1339, 1738
MaeI CTAG 3 cut(s) 690, 1196, 1311
MaeII ACGT 2 cut(s) 1260, 1725
MaeIII GTNAC 4 cut(s) 27, 73, 302, 523
MboII GAAGA 9 cut(s) 193, 220, 676, 846, 1013, 1376, 1490, 1497, 1563
MfeI CAATTG 1 cut(s) 401
MflI RGATCY 1 cut(s) 1141
MhlI GDGCHC 2 cut(s) 678, 1260
MlsI TGGCCA 1 cut(s) 627
MluCI AATT 8 cut(s) 146, 246, 401, 542, 632, 1016, 1099, 1488
MluNI TGGCCA 1 cut(s) 627
Mly113I GGCGCC 1 cut(s) 123
MlyI GAGTC 6 cut(s) 67, 89, 437, 1427, 1610, 1670
MmeI TCCRAC 5 cut(s) 63, 1056, 1161, 1720, 1734
Mox20I TGGCCA 1 cut(s) 627
MroXI GAANNNNTTC 1 cut(s) 1025
MscI TGGCCA 1 cut(s) 627
MseI TTAA 2 cut(s) 249, 1778
MslI CAYNNNNRTG 2 cut(s) 1136, 1269
Msp20I TGGCCA 1 cut(s) 627
MspCI CTTAAG 1 cut(s) 1777
MspI CCGG 2 cut(s) 71, 1128
MspR9I CCNGG 3 cut(s) 734, 779, 1539
MunI CAATTG 1 cut(s) 401
MvaI CCWGG 3 cut(s) 734, 779, 1539
MvnI CGCG 3 cut(s) 191, 1333, 1428
MwoI GCNNNNNNNGC 2 cut(s) 1685, 1769
NarI GGCGCC 1 cut(s) 123
NcoI CCATGG 2 cut(s) 235, 763
NlaIV GGNNCC 5 cut(s) 124, 1126, 1143, 1536, 1708
NmeAIII GCCGAG 2 cut(s) 87, 1699
NmuCI GTSAC 2 cut(s) 27, 73
PagI TCATGA 3 cut(s) 1108, 1207, 1639
PceI AGGCCT 1 cut(s) 452
PdmI GAANNNNTTC 1 cut(s) 1025
PfeI GAWTC 4 cut(s) 63, 117, 172, 983
PfoI TCCNGGA 1 cut(s) 777
PkrI GCNGC 7 cut(s) 1150, 1430, 1437, 1503, 1687, 1762, 1774
PleI GAGTC 6 cut(s) 66, 88, 437, 1426, 1609, 1669
PluTI GGCGCC 1 cut(s) 126
PmaCI CACGTG 1 cut(s) 1726
PmlI CACGTG 1 cut(s) 1726
PpsI GAGTC 6 cut(s) 66, 88, 437, 1426, 1609, 1669
Ppu21I YACGTR 1 cut(s) 1726
PshBI ATTAAT 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 678
Psp6I CCWGG 3 cut(s) 732, 777, 1537
PspCI CACGTG 1 cut(s) 1726
PspEI GGTNACC 1 cut(s) 73
PspFI CCCAGC 2 cut(s) 1592, 1714
PspGI CCWGG 3 cut(s) 732, 777, 1537
PspN4I GGNNCC 5 cut(s) 124, 1126, 1143, 1536, 1708
PspPI GGNCC 4 cut(s) 760, 956, 1652, 1707
PstI CTGCAG 2 cut(s) 1150, 1437
PsuI RGATCY 1 cut(s) 1141
RsaI GTAC 4 cut(s) 567, 1126, 1191, 1293
RsaNI GTAC 4 cut(s) 566, 1125, 1190, 1292
RseI CAYNNNNRTG 2 cut(s) 1136, 1269
SacI GAGCTC 1 cut(s) 678
SaqAI TTAA 2 cut(s) 249, 1778
SatI GCNGC 7 cut(s) 1149, 1429, 1436, 1502, 1686, 1761, 1773
Sau96I GGNCC 4 cut(s) 760, 956, 1652, 1707
ScaI AGTACT 1 cut(s) 567
SchI GAGTC 6 cut(s) 67, 89, 437, 1427, 1610, 1670
ScrFI CCNGG 3 cut(s) 734, 779, 1539
SduI GDGCHC 2 cut(s) 678, 1260
SfaNI GCATC 7 cut(s) 311, 352, 777, 811, 1140, 1339, 1738
SfcI CTRYAG 2 cut(s) 1146, 1433
SfoI GGCGCC 1 cut(s) 124
SinI GGWCC 3 cut(s) 956, 1652, 1707
SmiMI CAYNNNNRTG 2 cut(s) 1136, 1269
SmlI CTYRAG 4 cut(s) 746, 1119, 1324, 1777
SmoI CTYRAG 4 cut(s) 746, 1119, 1324, 1777
Sse9I AATT 8 cut(s) 146, 246, 401, 542, 632, 1016, 1099, 1488
SseBI AGGCCT 1 cut(s) 452
SsiI CCGC 3 cut(s) 890, 1428, 1685
SspDI GGCGCC 1 cut(s) 122
SspMI CTAG 3 cut(s) 690, 1196, 1311
SstI GAGCTC 1 cut(s) 678
StuI AGGCCT 1 cut(s) 452
StyD4I CCNGG 3 cut(s) 732, 777, 1537
StyI CCWWGG 3 cut(s) 235, 447, 763
TaaI ACNGT 2 cut(s) 280, 1361
TaiI ACGT 2 cut(s) 1263, 1728
TaqI TCGA 3 cut(s) 61, 165, 243
TasI AATT 8 cut(s) 146, 246, 401, 542, 632, 1016, 1099, 1488
TatI WGTACW 2 cut(s) 565, 1189
TauI GCSGC 2 cut(s) 1431, 1688
TfiI GAWTC 4 cut(s) 63, 117, 172, 983
Tru1I TTAA 2 cut(s) 249, 1778
Tru9I TTAA 2 cut(s) 249, 1778
TscAI CASTG 4 cut(s) 36, 57, 285, 1351
TseFI GTSAC 2 cut(s) 27, 73
TseI GCWGC 5 cut(s) 1148, 1435, 1501, 1760, 1772
Tsp45I GTSAC 2 cut(s) 27, 73
TspDTI ATGAA 8 cut(s) 339, 422, 822, 900, 1107, 1253, 1496, 1656
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 4 cut(s) 36, 57, 285, 1351
Vha464I CTTAAG 1 cut(s) 1777
VpaK11BI GGWCC 3 cut(s) 956, 1652, 1707
VspI ATTAAT 1 cut(s) 249
XbaI TCTAGA 1 cut(s) 1310
XmnI GAANNNNTTC 1 cut(s) 1025
XspI CTAG 3 cut(s) 690, 1196, 1311
ZrmI AGTACT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.