FvH4_3g42832

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009820
Physical Location & Seq
Reverse (-)
35808977 .. 35811606
2630 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42832.t1

Sequence Viewer

Length: 699 bp
ATGGAACTGGTGCGTTGGTCTACCTGGCGTGGTAATGGAACTGGTGCATTGGTTTACCTGGCTTGGCAATTTGGGTTGGTATTGGCTCTTGGTTTGGTTGTTGATCCCCAACTCCCAAGTGCTTTGCTTTCTGCTGTTTTTTGTAATGAATTCAATTCTAAGTCGTTGTTCTGGTCACGGACTGCAGGTGATGTCTCAATTGCTTATAGTATGCAAAAACTGGATTGTGAGTTGCCTCTCTCCTTCTTGGATTTAAAGTTTTGGTGGTGGCTGGCCACGTTTAGATGCAAAGCTTTAGTTATATTACATCTTGGTTCTCTGCTATTTTCAAATGCATGGGATAGCAAAACCTGTTATCTGTATGTGGATTTGATAACAATGAAGTTATCTGCTTTTGCAATTATAGATCACATGACAAAACCTGTGATGATGGTATTTGTGCAAGAATGCCTTGAGGAATGTAAGGATGCTGTTGCATCATTAATGGCTAGTGCAGCAAGATTATCTGATTTACCGGAGTTACGTGACATCAGGCATTTGTTTGAGAATATAGCTTCAAAGTCTCCCACAATTGAGAAGAAGGTTCAGTTGATGAAAGATATAGCATCAGAGTTTTCAATAAAGTGGGATTCCAAGTCTAAATCTATTATACAGGTTGTCACACTTTTGCAGGACAAACCATCTCAGACGCCCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

233

Amino Acids

26.24

Weight (kDa)

6.81

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 137 - 182 2.4e-06 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 176
Acc36I ACCTGC 1 cut(s) 176
AccI GTMKAC 1 cut(s) 20
AclWI GGATC 1 cut(s) 98
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 1 cut(s) 149
AcyI GRCGYC 1 cut(s) 689
AgsI TTSAA 4 cut(s) 154, 330, 558, 618
AjnI CCWGG 2 cut(s) 23, 57
AluBI AGCT 2 cut(s) 293, 554
AluI AGCT 2 cut(s) 293, 554
Alw26I GTCTC 2 cut(s) 199, 567
AlwI GGATC 1 cut(s) 98
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 1 cut(s) 494
ApoI RAATTY 1 cut(s) 149
AseI ATTAAT 1 cut(s) 482
AsuHPI GGTGA 1 cut(s) 200
BalI TGGCCA 1 cut(s) 275
BbvI GCAGC 1 cut(s) 506
BccI CCATC 2 cut(s) 424, 688
BciT130I CCWGG 2 cut(s) 25, 59
BcoDI GTCTC 2 cut(s) 199, 567
BfaI CTAG 1 cut(s) 489
BfmI CTRYAG 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 176
BisI GCNGC 1 cut(s) 495
BlsI GCNGC 1 cut(s) 496
Bme1390I CCNGG 2 cut(s) 25, 59
BmrFI CCNGG 2 cut(s) 25, 59
BmsI GCATC 4 cut(s) 275, 457, 485, 614
BpuEI CTTGAG 1 cut(s) 473
BsaAI YACGTR 1 cut(s) 524
BsaHI GRCGYC 1 cut(s) 689
BsaWI WCCGGW 1 cut(s) 514
Bse1I ACTGG 3 cut(s) 12, 46, 225
BseBI CCWGG 2 cut(s) 25, 59
BseGI GGATG 1 cut(s) 472
BseMII CTCAG 1 cut(s) 698
BseNI ACTGG 3 cut(s) 12, 46, 225
BseXI GCAGC 1 cut(s) 506
BsgI GTGCAG 1 cut(s) 513
BshFI GGCC 1 cut(s) 275
BsiSI CCGG 1 cut(s) 515
BsmAI GTCTC 2 cut(s) 199, 567
BsmI GAATGC 1 cut(s) 452
BsnI GGCC 1 cut(s) 275
Bsp143I GATC 2 cut(s) 103, 406
BspANI GGCC 1 cut(s) 275
BspCNI CTCAG 1 cut(s) 697
BspMAI CTGCAG 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 176
BspPI GGATC 1 cut(s) 98
BsrI ACTGG 3 cut(s) 12, 46, 225
BssMI GATC 2 cut(s) 103, 406
BssNI GRCGYC 1 cut(s) 689
Bst2UI CCWGG 2 cut(s) 25, 59
BstACI GRCGYC 1 cut(s) 689
BstBAI YACGTR 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 273
BstDEI CTNAG 2 cut(s) 159, 684
BstF5I GGATG 1 cut(s) 472
BstKTI GATC 2 cut(s) 106, 409
BstMAI GTCTC 2 cut(s) 199, 567
BstMBI GATC 2 cut(s) 103, 406
BstMWI GCNNNNNNNGC 1 cut(s) 494
BstNI CCWGG 2 cut(s) 25, 59
BstSCI CCNGG 2 cut(s) 23, 57
BstSFI CTRYAG 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 506
BsuRI GGCC 1 cut(s) 275
BtsCI GGATG 1 cut(s) 472
BveI ACCTGC 1 cut(s) 176
Cac8I GCNNGC 1 cut(s) 273
CviAII CATG 2 cut(s) 336, 412
CviJI RGCY 7 cut(s) 62, 86, 271, 275, 293, 488, 554
CviKI_1 RGCY 7 cut(s) 62, 86, 271, 275, 293, 488, 554
DdeI CTNAG 2 cut(s) 159, 684
DpnI GATC 2 cut(s) 105, 408
DpnII GATC 2 cut(s) 103, 406
DraI TTTAAA 1 cut(s) 255
EaeI YGGCCR 1 cut(s) 273
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 23, 57
EcoT22I ATGCAT 1 cut(s) 337
FaeI CATG 2 cut(s) 339, 415
FalI AAGNNNNNCTT 2 cut(s) 435, 467
FatI CATG 2 cut(s) 335, 411
FblI GTMKAC 1 cut(s) 20
Fnu4HI GCNGC 1 cut(s) 495
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 1 cut(s) 495
FspBI CTAG 1 cut(s) 489
GluI GCNGC 1 cut(s) 495
HaeIII GGCC 1 cut(s) 275
HapII CCGG 1 cut(s) 515
Hin1I GRCGYC 1 cut(s) 689
Hin1II CATG 2 cut(s) 339, 415
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 1 cut(s) 629
HpaII CCGG 1 cut(s) 515
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 21, 55
Hpy188I TCNGA 3 cut(s) 508, 610, 687
Hpy8I GTNNAC 2 cut(s) 21, 55
HpyAV CCTTC 2 cut(s) 253, 574
HpyCH4IV ACGT 2 cut(s) 278, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 494
HpyF3I CTNAG 2 cut(s) 159, 684
HpySE526I ACGT 2 cut(s) 278, 523
Hsp92I GRCGYC 1 cut(s) 689
Hsp92II CATG 2 cut(s) 339, 415
Kzo9I GATC 2 cut(s) 103, 406
Lsp1109I GCAGC 1 cut(s) 506
LweI GCATC 4 cut(s) 275, 457, 485, 614
MaeI CTAG 1 cut(s) 489
MaeII ACGT 2 cut(s) 278, 523
MaeIII GTNAC 4 cut(s) 174, 519, 524, 658
MalI GATC 2 cut(s) 105, 408
MboI GATC 2 cut(s) 103, 406
MboII GAAGA 1 cut(s) 589
MfeI CAATTG 2 cut(s) 198, 570
MlsI TGGCCA 1 cut(s) 275
MluCI AATT 6 cut(s) 68, 149, 154, 198, 399, 570
MluNI TGGCCA 1 cut(s) 275
MnlI CCTC 2 cut(s) 246, 448
Mox20I TGGCCA 1 cut(s) 275
Mph1103I ATGCAT 1 cut(s) 337
MscI TGGCCA 1 cut(s) 275
MseI TTAA 2 cut(s) 254, 482
Msp20I TGGCCA 1 cut(s) 275
MspI CCGG 1 cut(s) 515
MspR9I CCNGG 2 cut(s) 25, 59
MunI CAATTG 2 cut(s) 198, 570
Mva1269I GAATGC 1 cut(s) 452
MvaI CCWGG 2 cut(s) 25, 59
MwoI GCNNNNNNNGC 1 cut(s) 494
NdeII GATC 2 cut(s) 103, 406
NlaIII CATG 2 cut(s) 339, 415
NmuCI GTSAC 3 cut(s) 174, 524, 658
NsiI ATGCAT 1 cut(s) 337
PaqCI CACCTGC 1 cut(s) 176
PctI GAATGC 1 cut(s) 452
PfeI GAWTC 1 cut(s) 629
PkrI GCNGC 1 cut(s) 496
Ppu21I YACGTR 1 cut(s) 524
PshBI ATTAAT 1 cut(s) 482
Psp6I CCWGG 2 cut(s) 23, 57
PspGI CCWGG 2 cut(s) 23, 57
PstI CTGCAG 1 cut(s) 187
SaqAI TTAA 2 cut(s) 254, 482
SatI GCNGC 1 cut(s) 495
Sau3AI GATC 2 cut(s) 103, 406
ScrFI CCNGG 2 cut(s) 25, 59
SfaNI GCATC 4 cut(s) 275, 457, 485, 614
SfcI CTRYAG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 6 cut(s) 68, 149, 154, 198, 399, 570
SspMI CTAG 1 cut(s) 489
StyD4I CCNGG 2 cut(s) 23, 57
TaiI ACGT 2 cut(s) 281, 526
TasI AATT 6 cut(s) 68, 149, 154, 198, 399, 570
TfiI GAWTC 1 cut(s) 629
Tru1I TTAA 2 cut(s) 254, 482
Tru9I TTAA 2 cut(s) 254, 482
TseFI GTSAC 3 cut(s) 174, 524, 658
TseI GCWGC 1 cut(s) 494
Tsp45I GTSAC 3 cut(s) 174, 524, 658
TspDTI ATGAA 3 cut(s) 162, 395, 608
TspGWI ACGGA 1 cut(s) 193
VspI ATTAAT 1 cut(s) 482
XapI RAATTY 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 20
XspI CTAG 1 cut(s) 489
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.