Rh2DG054200

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009820
Physical Location & Seq
Forward (+)
3923808 .. 3927043
3236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG054200.1

Sequence Viewer

Length: 1614 bp
ATGCTCGACGGACTGCTCGGCCGTGGATTCGGCGCCAAGTGTAAATCGCTGATAAAATTGACGAAAACTCGGATCGATGTGATTCGGAGGAAGAGGAACGCGACGCAGAAGTTTTTGAAGAAAGATATAGCTGATCTACTCAACCATGGCCTCGAAATTAATGCTTTCGGAAGGTTTGTTGAGAACATAGCTTCAAAGCCTCCCACATTGGAAAAGAAGGTTCAATTGATGAAAGACATAGCATTAGAGTATTCAATAAAGTGGGACTCCAAGGCCTTTGAACAAATGATGTCTAAACCTCCTGCTTTTATACAGGACAAGCCAAAAACTTATGGGTCTTTCCCTGTTACTGACGATAAAGGTAAATTGTCTAATGGGAATGGTGCAGTACTAAAAGGAGATAGGCATGACCATCTGTCAACAGAAAGGAGACTTGATCCTAATGGTGGCCACAAATTACAGAACAACAAGGAGGTTATAGCCTTGAAGAAGGATGAGCTGGATGATCAGACTAGGCATAGACCTTTAGGAAAAGAATACAAGTCTCTCAAAGGCCGGGAAGGTAGTCTCAAGGAAAAAGAGGGCCATGGCATCTTGTTCCAGGAAAGGCAAGAAGTTGCTCACAGGTATGAAGCAAGAGAGGATGCTCCTTCAAAACCAGTTAGATTAGGCAGTTCTTCTCGGGGAAAAGGAGCAGAAAGGCATTATGAAGCAGAGAATAGTGTTCGTAAAAGAGACGACCCTGACACTATTCCTCATGTAAAGCCAAATGTTGCTGGACCACCTGTGAAAAGTGATGGTAAAGATTCTTTTGCTGGTAAAGAAGAAGGAACACCTAAATTGAAGCCCTTCTCCAACTATGGCCTTCCTCCTCCTTATGTGAAATCTAATGTCAGAGCAAAAGATAGAAAACATGAAACTAATTTCGGTCATGATTATTCTCAAGGTACCGGCATCTCTATGGATCCTGCAGCATATAAGAGAGCCATCGCTGATAATGTGTTGGAAAGGGTACAACTAGGTTGTGATCATGAGAGGCGTGCTACTGCGAAAGTTGATAGTTATGATCATGAGAAGGAGCTCGTTCATCAGGATGATATAGCTACCAACAGTGTACCAAAACCAAGATCATCTAGAAGGAGACCCTCAAGATCGCGCTCCTCTACCAATGATGCTGGCAACGGTGAAGATATAGGAGCTGCAACGAGCAAACCAAGAAGCCGGAGTAGGAGAAGGGATGAGTCAAGACGTGGCCTGCAACTGTTGTTTGATGATGACCGCCACCACAGGAAAGATGATGAAGAGAGAAGAATTGACGAACTGCTGCTTCATTACAGCACAAAGCCATCTAACTTGGAGCCAGGGCAGTCAAGAAGAAAGTCAAAAAGTCGTCATGCAAGCAGAGATAAGCCTGCTGGGGAGGAGTCTGAACCAACTCATGTAAGATCAACTTCCCTTCCTCATGAACATTCAGGTCCATCAGAGACGCCGAAAGTATTTGCTCGTGCGGCTTCCTTCCAACCAGATGGGTCCAACCCAGCTCGGCACGTGCATCCCAAGTTACCCGACTATGAGGATTTGGCTGCCAAGTTTGCAGCCTTAAGAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

537

Amino Acids

60.44

Weight (kDa)

9.7

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 63 4.2e-07 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 947
AccB1I GGYRCC 2 cut(s) 32, 947
AccII CGCG 2 cut(s) 101, 1156
AciI CCGC 2 cut(s) 1279, 1508
AclWI GGATC 4 cut(s) 80, 431, 959, 972
AcoI YGGCCR 2 cut(s) 19, 448
AcvI CACGTG 1 cut(s) 1549
AcyI GRCGYC 2 cut(s) 33, 1487
AfaI GTAC 4 cut(s) 390, 949, 1014, 1116
AfiI CCNNNNNNNGG 1 cut(s) 446
AflII CTTAAG 1 cut(s) 1600
AgsI TTSAA 8 cut(s) 118, 195, 224, 255, 281, 487, 654, 844
AhdI GACNNNNNGTC 1 cut(s) 415
AjiI CACGTC 1 cut(s) 1250
AjnI CCWGG 2 cut(s) 600, 1360
AluBI AGCT 7 cut(s) 131, 191, 499, 1081, 1103, 1199, 1541
AluI AGCT 7 cut(s) 131, 191, 499, 1081, 1103, 1199, 1541
Alw21I GWGCWC 1 cut(s) 1083
Alw26I GTCTC 6 cut(s) 424, 549, 572, 729, 1135, 1478
AlwI GGATC 4 cut(s) 80, 431, 959, 972
Ama87I CYCGRG 1 cut(s) 681
AoxI GGCC 8 cut(s) 19, 148, 273, 448, 553, 583, 862, 1252
ApeKI GCWGC 5 cut(s) 971, 1199, 1324, 1583, 1595
AseI ATTAAT 1 cut(s) 159
Asp700I GAANNNNTTC 1 cut(s) 848
Asp718I GGTACC 1 cut(s) 947
AspLEI GCGC 2 cut(s) 35, 1158
AspS9I GGNCC 4 cut(s) 583, 779, 1475, 1530
AsuC2I CCSGG 1 cut(s) 557
AsuHPI GGTGA 1 cut(s) 1196
AvaI CYCGRG 1 cut(s) 681
AvaII GGWCC 3 cut(s) 779, 1475, 1530
BalI TGGCCA 1 cut(s) 450
BamHI GGATCC 1 cut(s) 964
BanI GGYRCC 2 cut(s) 32, 947
BanII GRGCYC 1 cut(s) 1083
BauI CACGAG 1 cut(s) 1503
BbrPI CACGTG 1 cut(s) 1549
Bbv12I GWGCWC 1 cut(s) 1083
BbvI GCAGC 5 cut(s) 983, 1186, 1311, 1570, 1607
BccI CCATC 6 cut(s) 420, 791, 995, 1354, 1486, 1520
BceAI ACGGC 1 cut(s) 6
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 2 cut(s) 602, 1362
BclI TGATCA 3 cut(s) 505, 1027, 1066
BcnI CCSGG 1 cut(s) 557
BcoDI GTCTC 6 cut(s) 424, 549, 572, 729, 1135, 1478
BfaI CTAG 3 cut(s) 513, 1019, 1134
BfmI CTRYAG 1 cut(s) 969
BfoI RGCGCY 1 cut(s) 36
BfrI CTTAAG 1 cut(s) 1600
BisI GCNGC 6 cut(s) 972, 1200, 1325, 1509, 1584, 1596
BlsI GCNGC 6 cut(s) 973, 1201, 1326, 1510, 1585, 1597
BmcAI AGTACT 1 cut(s) 390
Bme1390I CCNGG 3 cut(s) 557, 602, 1362
Bme18I GGWCC 3 cut(s) 779, 1475, 1530
BmeRI GACNNNNNGTC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 681
BmgBI CACGTC 1 cut(s) 1250
BmgT120I GGNCC 4 cut(s) 583, 779, 1475, 1530
BmiI GGNNCC 5 cut(s) 34, 949, 966, 1359, 1531
BmrFI CCNGG 3 cut(s) 557, 602, 1362
BmsI GCATC 5 cut(s) 600, 634, 963, 1162, 1561
BpuEI CTTGAG 3 cut(s) 554, 927, 1132
BpuMI CCSGG 1 cut(s) 557
Bsa29I ATCGAT 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 1549
BsaHI GRCGYC 2 cut(s) 33, 1487
BsaI GGTCTC 1 cut(s) 1135
BsaJI CCNNGG 5 cut(s) 22, 145, 270, 586, 1361
Bsc4I CCNNNNNNNGG 1 cut(s) 446
Bse118I RCCGGY 1 cut(s) 950
Bse1I ACTGG 1 cut(s) 659
BseBI CCWGG 2 cut(s) 602, 1362
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 5 cut(s) 22, 145, 270, 586, 1361
BseGI GGATG 6 cut(s) 499, 508, 649, 1099, 1243, 1552
BseLI CCNNNNNNNGG 1 cut(s) 446
BseNI ACTGG 1 cut(s) 659
BseRI GAGGAG 3 cut(s) 861, 1150, 1436
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 5 cut(s) 983, 1186, 1311, 1570, 1607
BseYI CCCAGC 2 cut(s) 1415, 1537
BsgI GTGCAG 1 cut(s) 405
Bsh1236I CGCG 2 cut(s) 101, 1156
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 8 cut(s) 21, 150, 275, 450, 555, 585, 864, 1254
BshNI GGYRCC 2 cut(s) 32, 947
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 1083
BsiHKCI CYCGRG 1 cut(s) 681
BsiSI CCGG 3 cut(s) 556, 951, 1222
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 446
BsmAI GTCTC 6 cut(s) 424, 549, 572, 729, 1135, 1478
BsmBI CGTCTC 2 cut(s) 729, 1478
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 8 cut(s) 21, 150, 275, 450, 555, 585, 864, 1254
Bso31I GGTCTC 1 cut(s) 1135
BsoBI CYCGRG 1 cut(s) 681
Bsp1286I GDGCHC 1 cut(s) 1083
Bsp19I CCATGG 2 cut(s) 145, 586
BspACI CCGC 2 cut(s) 1279, 1508
BspANI GGCC 8 cut(s) 21, 150, 275, 450, 555, 585, 864, 1254
BspDI ATCGAT 1 cut(s) 75
BspFNI CGCG 2 cut(s) 101, 1156
BspHI TCATGA 4 cut(s) 931, 1030, 1069, 1462
BspLI GGNNCC 5 cut(s) 34, 949, 966, 1359, 1531
BspMAI CTGCAG 1 cut(s) 973
BspPI GGATC 4 cut(s) 80, 431, 959, 972
BspT107I GGYRCC 2 cut(s) 32, 947
BspTI CTTAAG 1 cut(s) 1600
BspTNI GGTCTC 1 cut(s) 1135
BsrFI RCCGGY 1 cut(s) 950
BsrI ACTGG 1 cut(s) 659
BssAI RCCGGY 1 cut(s) 950
BssECI CCNNGG 5 cut(s) 22, 145, 270, 586, 1361
BssNI GRCGYC 2 cut(s) 33, 1487
BssSI CACGAG 1 cut(s) 1503
BssT1I CCWWGG 3 cut(s) 145, 270, 586
Bst2BI CACGAG 1 cut(s) 1503
Bst2UI CCWGG 2 cut(s) 602, 1362
Bst4CI ACNGT 3 cut(s) 1112, 1184, 1263
Bst6I CTCTTC 2 cut(s) 86, 1296
BstACI GRCGYC 2 cut(s) 33, 1487
BstAFI CTTAAG 1 cut(s) 1600
BstBAI YACGTR 1 cut(s) 1549
BstC8I GCNNGC 5 cut(s) 1041, 1177, 1256, 1399, 1413
BstDSI CCRYGG 3 cut(s) 22, 145, 586
BstF5I GGATG 6 cut(s) 499, 508, 649, 1099, 1243, 1552
BstFNI CGCG 2 cut(s) 101, 1156
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 1158
BstMAI GTCTC 6 cut(s) 424, 549, 572, 729, 1135, 1478
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 2 cut(s) 1508, 1592
BstNI CCWGG 2 cut(s) 602, 1362
BstSCI CCNGG 3 cut(s) 555, 600, 1360
BstSFI CTRYAG 1 cut(s) 969
BstUI CGCG 2 cut(s) 101, 1156
BstV1I GCAGC 5 cut(s) 983, 1186, 1311, 1570, 1607
BstX2I RGATCY 1 cut(s) 964
BstXI CCANNNNNNTGG 1 cut(s) 1526
BstYI RGATCY 1 cut(s) 964
BstZI CGGCCG 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 8 cut(s) 21, 150, 275, 450, 555, 585, 864, 1254
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 3 cut(s) 22, 145, 586
BtgZI GCGATG 1 cut(s) 973
BtrI CACGTC 1 cut(s) 1250
BtsCI GGATG 6 cut(s) 499, 508, 649, 1099, 1243, 1552
BtsIMutI CAGTG 1 cut(s) 1117
Cac8I GCNNGC 5 cut(s) 1041, 1177, 1256, 1399, 1413
CciI TCATGA 4 cut(s) 931, 1030, 1069, 1462
CfoI GCGC 2 cut(s) 35, 1158
Cfr10I RCCGGY 1 cut(s) 950
Cfr13I GGNCC 4 cut(s) 583, 779, 1475, 1530
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 2 cut(s) 112, 1495
Csp6I GTAC 4 cut(s) 389, 948, 1013, 1115
CviQI GTAC 4 cut(s) 389, 948, 1013, 1115
DinI GGCGCC 1 cut(s) 34
DriI GACNNNNNGTC 1 cut(s) 415
EaeI YGGCCR 2 cut(s) 19, 448
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 2 cut(s) 86, 1296
Eam1105I GACNNNNNGTC 1 cut(s) 415
EarI CTCTTC 2 cut(s) 86, 1296
Ecl136II GAGCTC 1 cut(s) 1081
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 3 cut(s) 145, 270, 586
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 1083
Eco31I GGTCTC 1 cut(s) 1135
Eco47I GGWCC 3 cut(s) 779, 1475, 1530
Eco52I CGGCCG 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 1081
Eco72I CACGTG 1 cut(s) 1549
Eco88I CYCGRG 1 cut(s) 681
EcoICRI GAGCTC 1 cut(s) 1081
EcoRII CCWGG 2 cut(s) 600, 1360
EcoT14I CCWWGG 3 cut(s) 145, 270, 586
EcoT38I GRGCYC 1 cut(s) 1083
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 3 cut(s) 145, 270, 586
Esp3I CGTCTC 2 cut(s) 729, 1478
FalI AAGNNNNNCTT 2 cut(s) 1435, 1467
FaqI GGGAC 1 cut(s) 278
FbaI TGATCA 3 cut(s) 505, 1027, 1066
Fnu4HI GCNGC 6 cut(s) 972, 1200, 1325, 1509, 1584, 1596
FokI GGATG 6 cut(s) 506, 515, 656, 1106, 1250, 1539
FriOI GRGCYC 1 cut(s) 1083
Fsp4HI GCNGC 6 cut(s) 972, 1200, 1325, 1509, 1584, 1596
FspBI CTAG 3 cut(s) 513, 1019, 1134
GlaI GCGC 2 cut(s) 34, 1157
GluI GCNGC 6 cut(s) 972, 1200, 1325, 1509, 1584, 1596
GsaI CCCAGC 2 cut(s) 1419, 1541
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 8 cut(s) 21, 150, 275, 450, 555, 585, 864, 1254
HapII CCGG 3 cut(s) 556, 951, 1222
HgaI GACGC 2 cut(s) 112, 1495
HhaI GCGC 2 cut(s) 35, 1158
Hin1I GRCGYC 2 cut(s) 33, 1487
Hin6I GCGC 2 cut(s) 33, 1156
HinP1I GCGC 2 cut(s) 33, 1156
HincII GTYRAC 1 cut(s) 420
HindII GTYRAC 1 cut(s) 420
HinfI GANTC 6 cut(s) 27, 82, 266, 806, 1241, 1424
HpaII CCGG 3 cut(s) 556, 951, 1222
HphI GGTGA 1 cut(s) 1196
Hpy166II GTNNAC 2 cut(s) 420, 1115
Hpy188I TCNGA 7 cut(s) 72, 87, 170, 510, 896, 1429, 1483
Hpy188III TCNNGA 9 cut(s) 932, 1031, 1070, 1091, 1134, 1149, 1245, 1371, 1463
Hpy8I GTNNAC 2 cut(s) 420, 1115
Hpy99I CGWCG 2 cut(s) 11, 106
HpyCH4III ACNGT 3 cut(s) 1112, 1184, 1263
HpyCH4IV ACGT 2 cut(s) 1249, 1548
HpyCH4V TGCA 7 cut(s) 386, 971, 1202, 1258, 1397, 1552, 1595
HpyF10VI GCNNNNNNNGC 2 cut(s) 1508, 1592
HpySE526I ACGT 2 cut(s) 1249, 1548
Hsp92I GRCGYC 2 cut(s) 33, 1487
HspAI GCGC 2 cut(s) 33, 1156
KasI GGCGCC 1 cut(s) 32
KpnI GGTACC 1 cut(s) 951
Ksp22I TGATCA 3 cut(s) 505, 1027, 1066
LmnI GCTCC 6 cut(s) 652, 692, 1078, 1163, 1196, 1357
Lsp1109I GCAGC 5 cut(s) 983, 1186, 1311, 1570, 1607
LweI GCATC 5 cut(s) 600, 634, 963, 1162, 1561
MaeI CTAG 3 cut(s) 513, 1019, 1134
MaeII ACGT 2 cut(s) 1249, 1548
MaeIII GTNAC 2 cut(s) 346, 1560
MboII GAAGA 9 cut(s) 103, 130, 499, 669, 836, 1199, 1313, 1320, 1386
MfeI CAATTG 1 cut(s) 224
MflI RGATCY 1 cut(s) 964
MhlI GDGCHC 1 cut(s) 1083
MlsI TGGCCA 1 cut(s) 450
MluCI AATT 8 cut(s) 56, 156, 224, 365, 455, 839, 922, 1311
MluNI TGGCCA 1 cut(s) 450
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 3 cut(s) 260, 1250, 1433
MmeI TCCRAC 4 cut(s) 879, 984, 1543, 1557
Mox20I TGGCCA 1 cut(s) 450
MroXI GAANNNNTTC 1 cut(s) 848
MscI TGGCCA 1 cut(s) 450
MseI TTAA 2 cut(s) 159, 1601
MslI CAYNNNNRTG 3 cut(s) 627, 959, 1092
Msp20I TGGCCA 1 cut(s) 450
MspCI CTTAAG 1 cut(s) 1600
MspI CCGG 3 cut(s) 556, 951, 1222
MspR9I CCNGG 3 cut(s) 557, 602, 1362
MunI CAATTG 1 cut(s) 224
MvaI CCWGG 2 cut(s) 602, 1362
MvnI CGCG 2 cut(s) 101, 1156
MwoI GCNNNNNNNGC 2 cut(s) 1508, 1592
NarI GGCGCC 1 cut(s) 33
NciI CCSGG 1 cut(s) 557
NcoI CCATGG 2 cut(s) 145, 586
NlaIV GGNNCC 5 cut(s) 34, 949, 966, 1359, 1531
NmeAIII GCCGAG 1 cut(s) 1522
PagI TCATGA 4 cut(s) 931, 1030, 1069, 1462
PceI AGGCCT 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 848
PfeI GAWTC 3 cut(s) 27, 82, 806
PfoI TCCNGGA 1 cut(s) 600
PkrI GCNGC 6 cut(s) 973, 1201, 1326, 1510, 1585, 1597
PleI GAGTC 3 cut(s) 260, 1249, 1432
PluTI GGCGCC 1 cut(s) 36
PmaCI CACGTG 1 cut(s) 1549
PmlI CACGTG 1 cut(s) 1549
PpsI GAGTC 3 cut(s) 260, 1249, 1432
Ppu21I YACGTR 1 cut(s) 1549
PshBI ATTAAT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 1083
Psp6I CCWGG 2 cut(s) 600, 1360
PspCI CACGTG 1 cut(s) 1549
PspFI CCCAGC 2 cut(s) 1415, 1537
PspGI CCWGG 2 cut(s) 600, 1360
PspN4I GGNNCC 5 cut(s) 34, 949, 966, 1359, 1531
PspPI GGNCC 4 cut(s) 583, 779, 1475, 1530
PstI CTGCAG 1 cut(s) 973
PsuI RGATCY 1 cut(s) 964
RsaI GTAC 4 cut(s) 390, 949, 1014, 1116
RsaNI GTAC 4 cut(s) 389, 948, 1013, 1115
RseI CAYNNNNRTG 3 cut(s) 627, 959, 1092
SacI GAGCTC 1 cut(s) 1083
SaqAI TTAA 2 cut(s) 159, 1601
SatI GCNGC 6 cut(s) 972, 1200, 1325, 1509, 1584, 1596
Sau96I GGNCC 4 cut(s) 583, 779, 1475, 1530
ScaI AGTACT 1 cut(s) 390
SchI GAGTC 3 cut(s) 260, 1250, 1433
ScrFI CCNGG 3 cut(s) 557, 602, 1362
SduI GDGCHC 1 cut(s) 1083
SfaNI GCATC 5 cut(s) 600, 634, 963, 1162, 1561
SfcI CTRYAG 1 cut(s) 969
SfoI GGCGCC 1 cut(s) 34
SinI GGWCC 3 cut(s) 779, 1475, 1530
SmiMI CAYNNNNRTG 3 cut(s) 627, 959, 1092
SmlI CTYRAG 4 cut(s) 569, 942, 1147, 1600
SmoI CTYRAG 4 cut(s) 569, 942, 1147, 1600
Sse9I AATT 8 cut(s) 56, 156, 224, 365, 455, 839, 922, 1311
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 2 cut(s) 1279, 1508
SspDI GGCGCC 1 cut(s) 32
SspMI CTAG 3 cut(s) 513, 1019, 1134
SstI GAGCTC 1 cut(s) 1083
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 3 cut(s) 555, 600, 1360
StyI CCWWGG 3 cut(s) 145, 270, 586
TaaI ACNGT 3 cut(s) 1112, 1184, 1263
TaiI ACGT 2 cut(s) 1252, 1551
TaqI TCGA 3 cut(s) 6, 75, 153
TaqII GACCGA 1 cut(s) 917
TasI AATT 8 cut(s) 56, 156, 224, 365, 455, 839, 922, 1311
TatI WGTACW 1 cut(s) 388
TauI GCSGC 1 cut(s) 1511
TfiI GAWTC 3 cut(s) 27, 82, 806
Tru1I TTAA 2 cut(s) 159, 1601
Tru9I TTAA 2 cut(s) 159, 1601
TscAI CASTG 1 cut(s) 1117
TseI GCWGC 5 cut(s) 971, 1199, 1324, 1583, 1595
TspDTI ATGAA 8 cut(s) 245, 645, 723, 930, 1076, 1314, 1319, 1479
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 1 cut(s) 1117
Vha464I CTTAAG 1 cut(s) 1600
VpaK11BI GGWCC 3 cut(s) 779, 1475, 1530
VspI ATTAAT 1 cut(s) 159
XbaI TCTAGA 1 cut(s) 1133
XmnI GAANNNNTTC 1 cut(s) 848
XspI CTAG 3 cut(s) 513, 1019, 1134
ZrmI AGTACT 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.