Rroxscaffold_2G00151050

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009820
Physical Location & Seq
Reverse (-)
88359548 .. 88364797
5250 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151050.1

Sequence Viewer

Length: 1890 bp
ATGCTCGACGGACTGCTCGGCCGTGGTTTCGGCGCCAAGTGTAAATCGCTGATAAAATTGACGAAAACTCGGATCGATGTGATTCGGAGGAAGAGGAACGCGACGCAGAAGTTTTTGAAGAAAGATATAGCTGATCTACTCAACCACGGCCTCGAAATTAATGCTTTCGGAAGGAATTTAGGATCAATTTTGAAATATCCTGCTGAGGGACTTACAGTGGAGGTGATACTTTCGGCGTGTTACGATTTCGTTGAGCGATGCTGTGATTTGGTGTTGAAGCATCTTTCAGTCATGCAGAAACAAAGTGAATGCCCCGAGGACTGTAAGGAAGCTGTTGCATCACTGATGGTTGCTGCGGCAAGATTTTCTGATTTACCAGAGTTGCGTGACATTAGGCAACTGTTTCAAGAGAGATACGCGAATTCTCTGGATTACTGTGCGGATCAAAAGTTTGTTGAGAACATAGCTTCAAAGCCTCCCACATTGGAAAAGAAGGTTCAATTGATGAAAGACATAGCATTAGAGTATTCAATAAAGTGGGACTCCAAGGCCTTTGAACAAATGATGTCTAAACCTCCTGCTTTTATACAGGACAAGCCAAAAACTTATGGGTCTTTCCCTGTTACTGACGATAAAGGCAAATTGTCTAATGGGAATGGTGCAGTACTAAAAGGAGATAGGCATGACCATCTGTCAACGGAAAGGAGACTTGATCCTAATGGTGGCCACAAATTACAGAACAACAAGGAGGTTATAGCCTTGAAGAAGGATGAGCTCGATGATCAGACTAGGCATAGACCTTTAGGAAAAGAATACAAGTCTCTCAAAGGCCGGGAAGGTAGTCTCAAGGAAAAAGAGGGCCATGGCATCTTGTTCCAGGAAAGGCAAGAAGTTGCTCACAGGTATGAAGCAAGAGAGGATGCTCCTTCAAAACCAGTTAGATTAGGCAGTTCTTCTCGGGGAAAAGGAGCAGAAAGGCATTATGAAGCCGAGAATAGTGTTCGTAAAAGAGACGACCCCGACACTATTCCTCATGTAAAGCCAAATGTTGCTGGACCACCTGTGAAAAGTGATGGTAAAGATTCTTTTGCTGGTAAAGAAGAAGGAACACCTAAATTGAAGCCCTTCTCCAACTATGGCCTTCCTCCTCCTTATGTGAAATCTAATGTCAGAGCAAAAGATAGAAAACATGAAGCTAATTTCGGTCATGATTGTTCTCAAGGTTCCGGCATCTCTATGGATCCTGCAGCATATAAGAGAGCCTTTGCTGATAATGTGTTGGAAAGGGTACAACTAGGTCGTGATCATGAGAGGCGTGGTACTGCGAAAGTTGATAGTTATGATCATGAGAAGGAGCACGTTCATCAGGATGATATAGCTACCAACAATGTACCAAAACCAAGATCATCTAGAAGGAGACCCTCAAGATCGCGCTCCTCTACCAATGATGCTGGCAACGGTGAAGATATAGGAGCTGCAATGAGCAAACCAAGAAGCAGGAGTAGGAGAAGGGATGAGTCAAGACGTGGCCTGCAGCTGCTGTTTGATGATGACCACCACCACAGGAAAGATGATGAAGAGAGAAGAATTGACGAACTGCTGCTTCATTACAGCACAAAGCCATCTAACTTGGAGCCAGGGCAGTCAAGAAGAAAGTCAAAAAGTCGTCATGCAAGCAGAGATAAGCCTGCTGGGGAGGAGTCTGAACCAACTCACGTAAGATCAACTTCCCTTCCTCATGAACATTCAGGTCCATCAGAGACGCCGAAAGTATTTGCTCGTGCGGCTTCCTTCCAACCAGATGGGTCCAACCCAGCTCGGCACGTGCATCCCAAGTTGCCCGACTATGAGGATTTGGCTGCCAAGTTTGCAGCCTTGAGAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

629

Amino Acids

70.86

Weight (kDa)

9.34

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 179 2.4e-39 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 32
AccII CGCG 3 cut(s) 101, 419, 1432
AciI CCGC 3 cut(s) 356, 440, 1784
AclWI GGATC 6 cut(s) 80, 190, 450, 707, 1235, 1248
AcoI YGGCCR 2 cut(s) 19, 724
AcsI RAATTY 2 cut(s) 175, 421
AcvI CACGTG 1 cut(s) 1825
AcyI GRCGYC 2 cut(s) 33, 1763
AfaI GTAC 4 cut(s) 666, 1290, 1321, 1392
AfiI CCNNNNNNNGG 2 cut(s) 206, 722
AhdI GACNNNNNGTC 1 cut(s) 691
AjiI CACGTC 1 cut(s) 1526
AjnI CCWGG 2 cut(s) 876, 1636
AluBI AGCT 9 cut(s) 131, 332, 467, 775, 1196, 1379, 1475, 1537, 1817
AluI AGCT 9 cut(s) 131, 332, 467, 775, 1196, 1379, 1475, 1537, 1817
Alw21I GWGCWC 2 cut(s) 777, 1359
Alw26I GTCTC 6 cut(s) 700, 825, 848, 1005, 1411, 1754
AlwI GGATC 6 cut(s) 80, 190, 450, 707, 1235, 1248
AlwNI CAGNNNCTG 1 cut(s) 1540
Ama87I CYCGRG 2 cut(s) 314, 957
AoxI GGCC 8 cut(s) 19, 148, 549, 724, 829, 859, 1138, 1528
ApeKI GCWGC 8 cut(s) 353, 1247, 1475, 1534, 1537, 1600, 1859, 1871
ApoI RAATTY 2 cut(s) 175, 421
AseI ATTAAT 1 cut(s) 159
Asp700I GAANNNNTTC 1 cut(s) 1124
AspLEI GCGC 2 cut(s) 35, 1434
AspS9I GGNCC 4 cut(s) 859, 1055, 1751, 1806
AsuC2I CCSGG 1 cut(s) 833
AsuHPI GGTGA 2 cut(s) 235, 1472
AvaI CYCGRG 2 cut(s) 314, 957
AvaII GGWCC 3 cut(s) 1055, 1751, 1806
BalI TGGCCA 1 cut(s) 726
BamHI GGATCC 1 cut(s) 1240
BanI GGYRCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 777
BauI CACGAG 1 cut(s) 1779
BbrPI CACGTG 1 cut(s) 1825
Bbv12I GWGCWC 2 cut(s) 777, 1359
BbvCI CCTCAGC 1 cut(s) 204
BbvI GCAGC 8 cut(s) 340, 1259, 1462, 1524, 1546, 1587, 1846, 1883
BccI CCATC 6 cut(s) 340, 696, 1067, 1630, 1762, 1796
BceAI ACGGC 2 cut(s) 6, 163
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 2 cut(s) 878, 1638
BclI TGATCA 3 cut(s) 781, 1303, 1342
BcnI CCSGG 1 cut(s) 833
BcoDI GTCTC 6 cut(s) 700, 825, 848, 1005, 1411, 1754
BfaI CTAG 3 cut(s) 789, 1295, 1410
BfmI CTRYAG 2 cut(s) 1245, 1532
BfoI RGCGCY 1 cut(s) 36
BmcAI AGTACT 1 cut(s) 666
Bme1390I CCNGG 3 cut(s) 833, 878, 1638
Bme18I GGWCC 3 cut(s) 1055, 1751, 1806
BmeRI GACNNNNNGTC 1 cut(s) 691
BmeT110I CYCGRG 2 cut(s) 314, 957
BmgBI CACGTC 1 cut(s) 1526
BmgT120I GGNCC 4 cut(s) 859, 1055, 1751, 1806
BmiI GGNNCC 5 cut(s) 34, 1225, 1242, 1635, 1807
BmrFI CCNGG 3 cut(s) 833, 878, 1638
BmsI GCATC 8 cut(s) 248, 289, 347, 876, 910, 1239, 1438, 1837
Bpu10I CCTNAGC 1 cut(s) 204
BpuEI CTTGAG 3 cut(s) 830, 1203, 1408
BpuMI CCSGG 1 cut(s) 833
Bsa29I ATCGAT 1 cut(s) 75
BsaAI YACGTR 2 cut(s) 1717, 1825
BsaHI GRCGYC 2 cut(s) 33, 1763
BsaI GGTCTC 1 cut(s) 1411
BsaJI CCNNGG 6 cut(s) 22, 145, 315, 546, 862, 1637
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 722
Bse1I ACTGG 1 cut(s) 935
Bse3DI GCAATG 1 cut(s) 1485
BseBI CCWGG 2 cut(s) 878, 1638
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 6 cut(s) 22, 145, 315, 546, 862, 1637
BseGI GGATG 5 cut(s) 775, 925, 1375, 1519, 1828
BseLI CCNNNNNNNGG 2 cut(s) 206, 722
BseMI GCAATG 1 cut(s) 1485
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 935
BseRI GAGGAG 3 cut(s) 1137, 1426, 1712
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 8 cut(s) 340, 1259, 1462, 1524, 1546, 1587, 1846, 1883
BseYI CCCAGC 2 cut(s) 1691, 1813
BsgI GTGCAG 1 cut(s) 681
Bsh1236I CGCG 3 cut(s) 101, 419, 1432
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 8 cut(s) 21, 150, 551, 726, 831, 861, 1140, 1530
BshNI GGYRCC 1 cut(s) 32
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 2 cut(s) 777, 1359
BsiHKCI CYCGRG 2 cut(s) 314, 957
BsiSI CCGG 2 cut(s) 832, 1227
BslFI GGGAC 2 cut(s) 222, 554
BslI CCNNNNNNNGG 2 cut(s) 206, 722
BsmAI GTCTC 6 cut(s) 700, 825, 848, 1005, 1411, 1754
BsmBI CGTCTC 2 cut(s) 1005, 1754
BsmFI GGGAC 2 cut(s) 222, 554
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 8 cut(s) 21, 150, 551, 726, 831, 861, 1140, 1530
Bso31I GGTCTC 1 cut(s) 1411
BsoBI CYCGRG 2 cut(s) 314, 957
Bsp1286I GDGCHC 2 cut(s) 777, 1359
Bsp19I CCATGG 1 cut(s) 862
BspACI CCGC 3 cut(s) 356, 440, 1784
BspANI GGCC 8 cut(s) 21, 150, 551, 726, 831, 861, 1140, 1530
BspCNI CTCAG 1 cut(s) 196
BspDI ATCGAT 1 cut(s) 75
BspFNI CGCG 3 cut(s) 101, 419, 1432
BspHI TCATGA 4 cut(s) 1207, 1306, 1345, 1738
BspLI GGNNCC 5 cut(s) 34, 1225, 1242, 1635, 1807
BspMAI CTGCAG 2 cut(s) 1249, 1536
BspPI GGATC 6 cut(s) 80, 190, 450, 707, 1235, 1248
BspT107I GGYRCC 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 1411
BsrDI GCAATG 1 cut(s) 1485
BsrI ACTGG 1 cut(s) 935
BssECI CCNNGG 6 cut(s) 22, 145, 315, 546, 862, 1637
BssNI GRCGYC 2 cut(s) 33, 1763
BssSI CACGAG 1 cut(s) 1779
BssT1I CCWWGG 2 cut(s) 546, 862
Bst2BI CACGAG 1 cut(s) 1779
Bst2UI CCWGG 2 cut(s) 878, 1638
Bst4CI ACNGT 5 cut(s) 217, 323, 402, 437, 1460
Bst6I CTCTTC 2 cut(s) 86, 1572
BstACI GRCGYC 2 cut(s) 33, 1763
BstBAI YACGTR 2 cut(s) 1717, 1825
BstC8I GCNNGC 4 cut(s) 1453, 1532, 1675, 1689
BstDEI CTNAG 1 cut(s) 204
BstDSI CCRYGG 3 cut(s) 22, 145, 862
BstF5I GGATG 5 cut(s) 775, 925, 1375, 1519, 1828
BstFNI CGCG 3 cut(s) 101, 419, 1432
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 1434
BstMAI GTCTC 6 cut(s) 700, 825, 848, 1005, 1411, 1754
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 2 cut(s) 1784, 1868
BstNI CCWGG 2 cut(s) 878, 1638
BstSCI CCNGG 3 cut(s) 831, 876, 1636
BstSFI CTRYAG 2 cut(s) 1245, 1532
BstUI CGCG 3 cut(s) 101, 419, 1432
BstV1I GCAGC 8 cut(s) 340, 1259, 1462, 1524, 1546, 1587, 1846, 1883
BstX2I RGATCY 1 cut(s) 1240
BstXI CCANNNNNNTGG 1 cut(s) 1802
BstYI RGATCY 1 cut(s) 1240
BstZI CGGCCG 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 8 cut(s) 21, 150, 551, 726, 831, 861, 1140, 1530
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 3 cut(s) 22, 145, 862
BtgZI GCGATG 1 cut(s) 271
BtrI CACGTC 1 cut(s) 1526
BtsCI GGATG 5 cut(s) 775, 925, 1375, 1519, 1828
BtsIMutI CAGTG 2 cut(s) 222, 341
Cac8I GCNNGC 4 cut(s) 1453, 1532, 1675, 1689
CaiI CAGNNNCTG 1 cut(s) 1540
CciI TCATGA 4 cut(s) 1207, 1306, 1345, 1738
CfoI GCGC 2 cut(s) 35, 1434
Cfr13I GGNCC 4 cut(s) 859, 1055, 1751, 1806
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 2 cut(s) 112, 1771
Csp6I GTAC 4 cut(s) 665, 1289, 1320, 1391
CviQI GTAC 4 cut(s) 665, 1289, 1320, 1391
DdeI CTNAG 1 cut(s) 204
DinI GGCGCC 1 cut(s) 34
DriI GACNNNNNGTC 1 cut(s) 691
EaeI YGGCCR 2 cut(s) 19, 724
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 2 cut(s) 86, 1572
Eam1105I GACNNNNNGTC 1 cut(s) 691
EarI CTCTTC 2 cut(s) 86, 1572
Ecl136II GAGCTC 1 cut(s) 775
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 546, 862
Eco147I AGGCCT 1 cut(s) 551
Eco24I GRGCYC 1 cut(s) 777
Eco31I GGTCTC 1 cut(s) 1411
Eco47I GGWCC 3 cut(s) 1055, 1751, 1806
Eco52I CGGCCG 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 775
Eco72I CACGTG 1 cut(s) 1825
Eco88I CYCGRG 2 cut(s) 314, 957
EcoICRI GAGCTC 1 cut(s) 775
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 876, 1636
EcoT14I CCWWGG 2 cut(s) 546, 862
EcoT38I GRGCYC 1 cut(s) 777
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 2 cut(s) 546, 862
Esp3I CGTCTC 2 cut(s) 1005, 1754
FalI AAGNNNNNCTT 4 cut(s) 1247, 1279, 1711, 1743
FaqI GGGAC 2 cut(s) 222, 554
FbaI TGATCA 3 cut(s) 781, 1303, 1342
FokI GGATG 5 cut(s) 782, 932, 1382, 1526, 1815
FriOI GRGCYC 1 cut(s) 777
FspBI CTAG 3 cut(s) 789, 1295, 1410
GlaI GCGC 2 cut(s) 34, 1433
GsaI CCCAGC 2 cut(s) 1695, 1817
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 8 cut(s) 21, 150, 551, 726, 831, 861, 1140, 1530
HapII CCGG 2 cut(s) 832, 1227
HgaI GACGC 2 cut(s) 112, 1771
HhaI GCGC 2 cut(s) 35, 1434
Hin1I GRCGYC 2 cut(s) 33, 1763
Hin6I GCGC 2 cut(s) 33, 1432
HinP1I GCGC 2 cut(s) 33, 1432
HincII GTYRAC 1 cut(s) 696
HindII GTYRAC 1 cut(s) 696
HinfI GANTC 5 cut(s) 82, 542, 1082, 1517, 1700
HpaII CCGG 2 cut(s) 832, 1227
HphI GGTGA 2 cut(s) 235, 1472
Hpy166II GTNNAC 1 cut(s) 696
Hpy188I TCNGA 8 cut(s) 72, 87, 170, 370, 786, 1172, 1705, 1759
Hpy8I GTNNAC 1 cut(s) 696
Hpy99I CGWCG 2 cut(s) 11, 106
HpyCH4III ACNGT 5 cut(s) 217, 323, 402, 437, 1460
HpyCH4IV ACGT 4 cut(s) 1359, 1525, 1716, 1824
HpyCH4V TGCA 9 cut(s) 295, 338, 662, 1247, 1478, 1534, 1673, 1828, 1871
HpyF10VI GCNNNNNNNGC 2 cut(s) 1784, 1868
HpyF3I CTNAG 1 cut(s) 204
HpySE526I ACGT 4 cut(s) 1359, 1525, 1716, 1824
Hsp92I GRCGYC 2 cut(s) 33, 1763
HspAI GCGC 2 cut(s) 33, 1432
KasI GGCGCC 1 cut(s) 32
Ksp22I TGATCA 3 cut(s) 781, 1303, 1342
LmnI GCTCC 6 cut(s) 928, 968, 1354, 1439, 1472, 1633
Lsp1109I GCAGC 8 cut(s) 340, 1259, 1462, 1524, 1546, 1587, 1846, 1883
LweI GCATC 8 cut(s) 248, 289, 347, 876, 910, 1239, 1438, 1837
MaeI CTAG 3 cut(s) 789, 1295, 1410
MaeII ACGT 4 cut(s) 1359, 1525, 1716, 1824
MaeIII GTNAC 3 cut(s) 239, 386, 622
MboII GAAGA 9 cut(s) 103, 130, 775, 945, 1112, 1475, 1589, 1596, 1662
MfeI CAATTG 1 cut(s) 500
MflI RGATCY 1 cut(s) 1240
MhlI GDGCHC 2 cut(s) 777, 1359
MlsI TGGCCA 1 cut(s) 726
MluNI TGGCCA 1 cut(s) 726
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 3 cut(s) 536, 1526, 1709
MmeI TCCRAC 4 cut(s) 1155, 1260, 1819, 1833
Mox20I TGGCCA 1 cut(s) 726
MroXI GAANNNNTTC 1 cut(s) 1124
MscI TGGCCA 1 cut(s) 726
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 3 cut(s) 903, 1235, 1368
Msp20I TGGCCA 1 cut(s) 726
MspA1I CMGCKG 1 cut(s) 1537
MspI CCGG 2 cut(s) 832, 1227
MspR9I CCNGG 3 cut(s) 833, 878, 1638
MunI CAATTG 1 cut(s) 500
Mva1269I GAATGC 1 cut(s) 314
MvaI CCWGG 2 cut(s) 878, 1638
MvnI CGCG 3 cut(s) 101, 419, 1432
MwoI GCNNNNNNNGC 2 cut(s) 1784, 1868
NarI GGCGCC 1 cut(s) 33
NciI CCSGG 1 cut(s) 833
NcoI CCATGG 1 cut(s) 862
NlaIV GGNNCC 5 cut(s) 34, 1225, 1242, 1635, 1807
NmeAIII GCCGAG 2 cut(s) 1015, 1798
NmuCI GTSAC 1 cut(s) 386
PagI TCATGA 4 cut(s) 1207, 1306, 1345, 1738
PceI AGGCCT 1 cut(s) 551
PctI GAATGC 1 cut(s) 314
PdmI GAANNNNTTC 1 cut(s) 1124
PfeI GAWTC 2 cut(s) 82, 1082
PfoI TCCNGGA 1 cut(s) 876
PleI GAGTC 3 cut(s) 536, 1525, 1708
PluTI GGCGCC 1 cut(s) 36
PmaCI CACGTG 1 cut(s) 1825
PmlI CACGTG 1 cut(s) 1825
PpsI GAGTC 3 cut(s) 536, 1525, 1708
Ppu21I YACGTR 2 cut(s) 1717, 1825
PshBI ATTAAT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 777
Psp6I CCWGG 2 cut(s) 876, 1636
PspCI CACGTG 1 cut(s) 1825
PspFI CCCAGC 2 cut(s) 1691, 1813
PspGI CCWGG 2 cut(s) 876, 1636
PspN4I GGNNCC 5 cut(s) 34, 1225, 1242, 1635, 1807
PspPI GGNCC 4 cut(s) 859, 1055, 1751, 1806
PstI CTGCAG 2 cut(s) 1249, 1536
PstNI CAGNNNCTG 1 cut(s) 1540
PsuI RGATCY 1 cut(s) 1240
PvuII CAGCTG 1 cut(s) 1537
RsaI GTAC 4 cut(s) 666, 1290, 1321, 1392
RsaNI GTAC 4 cut(s) 665, 1289, 1320, 1391
RseI CAYNNNNRTG 3 cut(s) 903, 1235, 1368
SacI GAGCTC 1 cut(s) 777
SaqAI TTAA 1 cut(s) 159
Sau96I GGNCC 4 cut(s) 859, 1055, 1751, 1806
ScaI AGTACT 1 cut(s) 666
SchI GAGTC 3 cut(s) 536, 1526, 1709
ScrFI CCNGG 3 cut(s) 833, 878, 1638
SduI GDGCHC 2 cut(s) 777, 1359
SfaNI GCATC 8 cut(s) 248, 289, 347, 876, 910, 1239, 1438, 1837
SfcI CTRYAG 2 cut(s) 1245, 1532
SfoI GGCGCC 1 cut(s) 34
SinI GGWCC 3 cut(s) 1055, 1751, 1806
SmiMI CAYNNNNRTG 3 cut(s) 903, 1235, 1368
SmlI CTYRAG 4 cut(s) 845, 1218, 1423, 1876
SmoI CTYRAG 4 cut(s) 845, 1218, 1423, 1876
SseBI AGGCCT 1 cut(s) 551
SsiI CCGC 3 cut(s) 356, 440, 1784
SspDI GGCGCC 1 cut(s) 32
SspMI CTAG 3 cut(s) 789, 1295, 1410
SstI GAGCTC 1 cut(s) 777
StuI AGGCCT 1 cut(s) 551
StyD4I CCNGG 3 cut(s) 831, 876, 1636
StyI CCWWGG 2 cut(s) 546, 862
TaaI ACNGT 5 cut(s) 217, 323, 402, 437, 1460
TaiI ACGT 4 cut(s) 1362, 1528, 1719, 1827
TaqI TCGA 4 cut(s) 6, 75, 153, 777
TaqII GACCGA 1 cut(s) 1193
TatI WGTACW 1 cut(s) 664
TauI GCSGC 2 cut(s) 359, 1787
TfiI GAWTC 2 cut(s) 82, 1082
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 2 cut(s) 222, 348
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 8 cut(s) 353, 1247, 1475, 1534, 1537, 1600, 1859, 1871
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 8 cut(s) 521, 921, 999, 1206, 1352, 1590, 1595, 1755
TspGWI ACGGA 2 cut(s) 24, 713
TspRI CASTG 2 cut(s) 222, 348
VpaK11BI GGWCC 3 cut(s) 1055, 1751, 1806
VspI ATTAAT 1 cut(s) 159
XapI RAATTY 2 cut(s) 175, 421
XbaI TCTAGA 1 cut(s) 1409
XmnI GAANNNNTTC 1 cut(s) 1124
XspI CTAG 3 cut(s) 789, 1295, 1410
ZrmI AGTACT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.