Rh2BG053500

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009820
Physical Location & Seq
Forward (+)
3810010 .. 3814312
4303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG053500.1

Sequence Viewer

Length: 1617 bp
ATGTTTACTTTGCTCCATTATTGTCATTTCAGTGAATGCCCTGAGGACTGTAAGGAAGCTGTTGCATCACTGATGGTTGCTGCGGCAAGATTTTCTGATTTACCAGAGTTGCGTGACATTAGGCAACTATTTCAAGAGAGATACGCGAATTCTTTGGATTACTGTGCGGATCAAAAGTTTGTCGAGAACATAGCTTCCAAGCCTCCCACATTGGAAAAGAAGGTTCAATTGATGAAAGACATAGCATCAGAGTATTCAATAAAGTGGGACTCCAAGGCCTTTGAACAAATGATGTCTAAACCTCCTGCTTTTATACAGGACAAGCCAAAAACTTATGGGTCTTTCCCTGTTACTGACGATAAAGGCAAATTGTCTAATGGGAATGGTGCAGTACTAAAAGGAGATAGGCATGACCATCTGTCAACAGAAAGGAGACTTGATCCTAATGGTGGCCACAAATTACAGAACAACAAGGAGGTTATAGCCTTGAAGAAGGATGAGCTCGATGATCAGACTAGGCATAGACCTTTAGGAAAAGAATACAAGTCTCTCAAAGGCCGGGAAGGTAGTCTCAAGGAAAAAGAGGGCCATGGCATCTTGTTCCAGGAAAGGCAAGAAGTTGCTCACAGGTATGAAGCAAGAGAGGATGCTCCTTCAAAACCAGTTAGATTAGGCAGTTCTTCTCGGGGAAAAGGAGCAGAAAGGCATTATGAAGCAGAGAATAGTGTTCGTAAAAGAGACGACCCCGACACTATTCCTCATGTAAAGCCAAATGTTGCCGGACCACCTGTGAAAAGTGATGGTAAAGATTCTTTTGCTGGTAAAGAAGAAGGAACACGTAAATTGAAGCCCTTCTCCAACTATGGCCTTCCTCCTCCTTATGTGAAATCTAATGTCAGAGCAAAAGATAGAAAACATGAAGCTAATTTCGGTCATGATTGTTCTCAAGGTACTGGCATCTCTATGGATCCTGCAGCATATAAGAGAGCCTTTGCTGATAATGTGTTGGAAAGGGTACAACTAGGTCGTGATCATGAGAGGCGTGCTACTGCAAAAGTTGATAGTTATGATCATGAGAAGGGGCACATTCATCAGGATGATATAGCTACCAACAATGTACCAAAACCAAGATCATCTAGAAGGAGACCCTCAAGATCGCGCTCCTCTACCAATGATGCTGGCAACGGTGAAGATATAGGAGCTGCAATGAGCAAACCAAGGAGCAGGAGTAGGAGAAGGGATGAGTCAAGACGTGGCCTGCAGCTGTTGTTTGATGATGACCACCACCACAGGAAAGATGATGAAGAGAGAAGAATTGATGAACTGCTGCTTCATTACAGCACAAAGCCATCTAACTTGGAGCCAGGGCAGTCAAGAAGAAAGTCAAAAAGTCGTCATGCAAGCAGAGATAAGCCTGCTGGGGAGGAGTCTGAACCAACTCATGTAAGATCAACTTCCCTTCCTCATGAACATTCAGGTCCATCAGAGACGCCCAAAGTATTTGCTCGTGCGGCTTCCTTCCAACCAGATGGGTCCAACCCAGCTCGGCATGTGCATCCCAAGTTGCCCGACTATGAGGATTTGGCTGCCAAGTTTGCAGCCTTAAGAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

538

Amino Acids

60.81

Weight (kDa)

9.27

Isoelectric Point (pI)

52.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 11 - 88 4.7e-17 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 146, 1159
AciI CCGC 3 cut(s) 83, 167, 1511
AclWI GGATC 4 cut(s) 177, 434, 962, 975
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 1 cut(s) 148
AcyI GRCGYC 1 cut(s) 1490
AfaI GTAC 4 cut(s) 393, 952, 1017, 1119
AfiI CCNNNNNNNGG 1 cut(s) 449
AflII CTTAAG 1 cut(s) 1603
AflIII ACRYGT 1 cut(s) 836
AgsI TTSAA 7 cut(s) 134, 227, 258, 284, 490, 657, 847
AhdI GACNNNNNGTC 1 cut(s) 418
AjiI CACGTC 1 cut(s) 1253
AjnI CCWGG 2 cut(s) 603, 1363
AloI GAACNNNNNNTCC 2 cut(s) 179, 211
AluBI AGCT 8 cut(s) 59, 194, 502, 923, 1106, 1202, 1264, 1544
AluI AGCT 8 cut(s) 59, 194, 502, 923, 1106, 1202, 1264, 1544
Alw21I GWGCWC 1 cut(s) 504
Alw26I GTCTC 6 cut(s) 427, 552, 575, 732, 1138, 1481
AlwI GGATC 4 cut(s) 177, 434, 962, 975
Ama87I CYCGRG 1 cut(s) 684
AoxI GGCC 6 cut(s) 276, 451, 556, 586, 865, 1255
ApeKI GCWGC 7 cut(s) 80, 974, 1202, 1261, 1327, 1586, 1598
ApoI RAATTY 1 cut(s) 148
Asp700I GAANNNNTTC 1 cut(s) 851
AspLEI GCGC 1 cut(s) 1161
AspS9I GGNCC 4 cut(s) 586, 782, 1478, 1533
AsuC2I CCSGG 1 cut(s) 560
AsuHPI GGTGA 1 cut(s) 1199
AvaI CYCGRG 1 cut(s) 684
AvaII GGWCC 3 cut(s) 782, 1478, 1533
AxyI CCTNAGG 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 1086
BalI TGGCCA 1 cut(s) 453
BamHI GGATCC 1 cut(s) 967
BanII GRGCYC 1 cut(s) 504
BauI CACGAG 1 cut(s) 1506
Bbv12I GWGCWC 1 cut(s) 504
BbvI GCAGC 7 cut(s) 67, 986, 1189, 1273, 1314, 1573, 1610
BccI CCATC 6 cut(s) 67, 423, 794, 1357, 1489, 1523
BciT130I CCWGG 2 cut(s) 605, 1365
BclI TGATCA 3 cut(s) 508, 1030, 1069
BcnI CCSGG 1 cut(s) 560
BcoDI GTCTC 6 cut(s) 427, 552, 575, 732, 1138, 1481
BfaI CTAG 3 cut(s) 516, 1022, 1137
BfmI CTRYAG 2 cut(s) 972, 1259
BfrI CTTAAG 1 cut(s) 1603
BisI GCNGC 9 cut(s) 81, 84, 975, 1203, 1262, 1328, 1512, 1587, 1599
BlsI GCNGC 9 cut(s) 82, 85, 976, 1204, 1263, 1329, 1513, 1588, 1600
BmcAI AGTACT 1 cut(s) 393
Bme1390I CCNGG 3 cut(s) 560, 605, 1365
Bme18I GGWCC 3 cut(s) 782, 1478, 1533
BmeRI GACNNNNNGTC 1 cut(s) 418
BmeT110I CYCGRG 1 cut(s) 684
BmgBI CACGTC 1 cut(s) 1253
BmgT120I GGNCC 4 cut(s) 586, 782, 1478, 1533
BmiI GGNNCC 3 cut(s) 969, 1362, 1534
BmrFI CCNGG 3 cut(s) 560, 605, 1365
BmsI GCATC 7 cut(s) 74, 254, 603, 637, 966, 1165, 1564
BpuEI CTTGAG 3 cut(s) 557, 930, 1135
BpuMI CCSGG 1 cut(s) 560
BsaAI YACGTR 1 cut(s) 839
BsaHI GRCGYC 1 cut(s) 1490
BsaI GGTCTC 1 cut(s) 1138
BsaJI CCNNGG 4 cut(s) 273, 589, 1217, 1364
BsaXI ACNNNNNCTCC 2 cut(s) 1213, 1243
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse1I ACTGG 2 cut(s) 662, 958
Bse21I CCTNAGG 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 1212
BseBI CCWGG 2 cut(s) 605, 1365
BseDI CCNNGG 4 cut(s) 273, 589, 1217, 1364
BseGI GGATG 5 cut(s) 502, 652, 1102, 1246, 1555
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMI GCAATG 1 cut(s) 1212
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 2 cut(s) 662, 958
BseRI GAGGAG 3 cut(s) 864, 1153, 1439
BseSI GKGCMC 1 cut(s) 1086
BseXI GCAGC 7 cut(s) 67, 986, 1189, 1273, 1314, 1573, 1610
BseYI CCCAGC 2 cut(s) 1418, 1540
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 2 cut(s) 146, 1159
BshFI GGCC 6 cut(s) 278, 453, 558, 588, 867, 1257
BsiHKAI GWGCWC 1 cut(s) 504
BsiHKCI CYCGRG 1 cut(s) 684
BsiSI CCGG 2 cut(s) 559, 780
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 6 cut(s) 427, 552, 575, 732, 1138, 1481
BsmBI CGTCTC 2 cut(s) 732, 1481
BsmFI GGGAC 1 cut(s) 281
BsmI GAATGC 1 cut(s) 41
BsnI GGCC 6 cut(s) 278, 453, 558, 588, 867, 1257
Bso31I GGTCTC 1 cut(s) 1138
BsoBI CYCGRG 1 cut(s) 684
Bsp1286I GDGCHC 2 cut(s) 504, 1086
Bsp143I GATC 9 cut(s) 169, 439, 508, 967, 1030, 1069, 1130, 1154, 1448
Bsp19I CCATGG 1 cut(s) 589
BspACI CCGC 3 cut(s) 83, 167, 1511
BspANI GGCC 6 cut(s) 278, 453, 558, 588, 867, 1257
BspCNI CTCAG 1 cut(s) 34
BspFNI CGCG 2 cut(s) 146, 1159
BspHI TCATGA 4 cut(s) 934, 1033, 1072, 1465
BspLI GGNNCC 3 cut(s) 969, 1362, 1534
BspMAI CTGCAG 2 cut(s) 976, 1263
BspPI GGATC 4 cut(s) 177, 434, 962, 975
BspTI CTTAAG 1 cut(s) 1603
BspTNI GGTCTC 1 cut(s) 1138
BsrDI GCAATG 1 cut(s) 1212
BsrI ACTGG 2 cut(s) 662, 958
BssECI CCNNGG 4 cut(s) 273, 589, 1217, 1364
BssMI GATC 9 cut(s) 169, 439, 508, 967, 1030, 1069, 1130, 1154, 1448
BssNI GRCGYC 1 cut(s) 1490
BssSI CACGAG 1 cut(s) 1506
BssT1I CCWWGG 3 cut(s) 273, 589, 1217
Bst2BI CACGAG 1 cut(s) 1506
Bst2UI CCWGG 2 cut(s) 605, 1365
Bst4CI ACNGT 3 cut(s) 50, 164, 1187
Bst6I CTCTTC 1 cut(s) 1299
BstACI GRCGYC 1 cut(s) 1490
BstAFI CTTAAG 1 cut(s) 1603
BstBAI YACGTR 1 cut(s) 839
BstC8I GCNNGC 5 cut(s) 1044, 1180, 1259, 1402, 1416
BstDEI CTNAG 1 cut(s) 42
BstDSI CCRYGG 1 cut(s) 589
BstF5I GGATG 5 cut(s) 502, 652, 1102, 1246, 1555
BstFNI CGCG 2 cut(s) 146, 1159
BstHHI GCGC 1 cut(s) 1161
BstKTI GATC 9 cut(s) 172, 442, 511, 970, 1033, 1072, 1133, 1157, 1451
BstMAI GTCTC 6 cut(s) 427, 552, 575, 732, 1138, 1481
BstMBI GATC 9 cut(s) 169, 439, 508, 967, 1030, 1069, 1130, 1154, 1448
BstMWI GCNNNNNNNGC 2 cut(s) 1511, 1595
BstNI CCWGG 2 cut(s) 605, 1365
BstNSI RCATGY 1 cut(s) 1553
BstSCI CCNGG 3 cut(s) 558, 603, 1363
BstSFI CTRYAG 2 cut(s) 972, 1259
BstSLI GKGCMC 1 cut(s) 1086
BstUI CGCG 2 cut(s) 146, 1159
BstV1I GCAGC 7 cut(s) 67, 986, 1189, 1273, 1314, 1573, 1610
BstX2I RGATCY 1 cut(s) 967
BstXI CCANNNNNNTGG 1 cut(s) 1529
BstYI RGATCY 1 cut(s) 967
Bsu36I CCTNAGG 1 cut(s) 42
BsuRI GGCC 6 cut(s) 278, 453, 558, 588, 867, 1257
BtgI CCRYGG 1 cut(s) 589
BtrI CACGTC 1 cut(s) 1253
BtsCI GGATG 5 cut(s) 502, 652, 1102, 1246, 1555
BtsIMutI CAGTG 2 cut(s) 37, 68
Cac8I GCNNGC 5 cut(s) 1044, 1180, 1259, 1402, 1416
CciI TCATGA 4 cut(s) 934, 1033, 1072, 1465
CfoI GCGC 1 cut(s) 1161
Cfr13I GGNCC 4 cut(s) 586, 782, 1478, 1533
CseI GACGC 1 cut(s) 1498
Csp6I GTAC 4 cut(s) 392, 951, 1016, 1118
CviQI GTAC 4 cut(s) 392, 951, 1016, 1118
DdeI CTNAG 1 cut(s) 42
DpnI GATC 9 cut(s) 171, 441, 510, 969, 1032, 1071, 1132, 1156, 1450
DpnII GATC 9 cut(s) 169, 439, 508, 967, 1030, 1069, 1130, 1154, 1448
DriI GACNNNNNGTC 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 451
Eam1104I CTCTTC 1 cut(s) 1299
Eam1105I GACNNNNNGTC 1 cut(s) 418
EarI CTCTTC 1 cut(s) 1299
Ecl136II GAGCTC 1 cut(s) 502
Eco130I CCWWGG 3 cut(s) 273, 589, 1217
Eco147I AGGCCT 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 504
Eco31I GGTCTC 1 cut(s) 1138
Eco47I GGWCC 3 cut(s) 782, 1478, 1533
Eco53kI GAGCTC 1 cut(s) 502
Eco81I CCTNAGG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 684
EcoICRI GAGCTC 1 cut(s) 502
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 603, 1363
EcoT14I CCWWGG 3 cut(s) 273, 589, 1217
EcoT38I GRGCYC 1 cut(s) 504
ErhI CCWWGG 3 cut(s) 273, 589, 1217
Esp3I CGTCTC 2 cut(s) 732, 1481
FalI AAGNNNNNCTT 4 cut(s) 974, 1006, 1438, 1470
FaqI GGGAC 1 cut(s) 281
FbaI TGATCA 3 cut(s) 508, 1030, 1069
Fnu4HI GCNGC 9 cut(s) 81, 84, 975, 1203, 1262, 1328, 1512, 1587, 1599
FokI GGATG 5 cut(s) 509, 659, 1109, 1253, 1542
FriOI GRGCYC 1 cut(s) 504
Fsp4HI GCNGC 9 cut(s) 81, 84, 975, 1203, 1262, 1328, 1512, 1587, 1599
FspBI CTAG 3 cut(s) 516, 1022, 1137
GlaI GCGC 1 cut(s) 1160
GluI GCNGC 9 cut(s) 81, 84, 975, 1203, 1262, 1328, 1512, 1587, 1599
GsaI CCCAGC 2 cut(s) 1422, 1544
HaeIII GGCC 6 cut(s) 278, 453, 558, 588, 867, 1257
HapII CCGG 2 cut(s) 559, 780
HgaI GACGC 1 cut(s) 1498
HhaI GCGC 1 cut(s) 1161
Hin1I GRCGYC 1 cut(s) 1490
Hin6I GCGC 1 cut(s) 1159
HinP1I GCGC 1 cut(s) 1159
HincII GTYRAC 1 cut(s) 423
HindII GTYRAC 1 cut(s) 423
HinfI GANTC 4 cut(s) 269, 809, 1244, 1427
HpaII CCGG 2 cut(s) 559, 780
HphI GGTGA 1 cut(s) 1199
Hpy166II GTNNAC 2 cut(s) 6, 423
Hpy188I TCNGA 6 cut(s) 97, 250, 513, 899, 1432, 1486
Hpy8I GTNNAC 2 cut(s) 6, 423
HpyCH4III ACNGT 3 cut(s) 50, 164, 1187
HpyCH4IV ACGT 2 cut(s) 838, 1252
HpyCH4V TGCA 9 cut(s) 65, 389, 974, 1052, 1205, 1261, 1400, 1555, 1598
HpyF10VI GCNNNNNNNGC 2 cut(s) 1511, 1595
HpyF3I CTNAG 1 cut(s) 42
HpySE526I ACGT 2 cut(s) 838, 1252
Hsp92I GRCGYC 1 cut(s) 1490
HspAI GCGC 1 cut(s) 1159
Ksp22I TGATCA 3 cut(s) 508, 1030, 1069
Kzo9I GATC 9 cut(s) 169, 439, 508, 967, 1030, 1069, 1130, 1154, 1448
LmnI GCTCC 7 cut(s) 18, 655, 695, 1166, 1199, 1221, 1360
Lsp1109I GCAGC 7 cut(s) 67, 986, 1189, 1273, 1314, 1573, 1610
LweI GCATC 7 cut(s) 74, 254, 603, 637, 966, 1165, 1564
MaeI CTAG 3 cut(s) 516, 1022, 1137
MaeII ACGT 2 cut(s) 838, 1252
MaeIII GTNAC 2 cut(s) 113, 349
MalI GATC 9 cut(s) 171, 441, 510, 969, 1032, 1071, 1132, 1156, 1450
MboI GATC 9 cut(s) 169, 439, 508, 967, 1030, 1069, 1130, 1154, 1448
MboII GAAGA 7 cut(s) 502, 672, 839, 1202, 1316, 1323, 1389
MfeI CAATTG 1 cut(s) 227
MflI RGATCY 1 cut(s) 967
MhlI GDGCHC 2 cut(s) 504, 1086
MlsI TGGCCA 1 cut(s) 453
MluCI AATT 7 cut(s) 148, 227, 368, 458, 842, 925, 1314
MluNI TGGCCA 1 cut(s) 453
MlyI GAGTC 3 cut(s) 263, 1253, 1436
MmeI TCCRAC 4 cut(s) 882, 987, 1546, 1560
Mox20I TGGCCA 1 cut(s) 453
MroXI GAANNNNTTC 1 cut(s) 851
MscI TGGCCA 1 cut(s) 453
MseI TTAA 1 cut(s) 1604
MslI CAYNNNNRTG 4 cut(s) 30, 630, 962, 1095
Msp20I TGGCCA 1 cut(s) 453
MspA1I CMGCKG 1 cut(s) 1264
MspCI CTTAAG 1 cut(s) 1603
MspI CCGG 2 cut(s) 559, 780
MspR9I CCNGG 3 cut(s) 560, 605, 1365
MunI CAATTG 1 cut(s) 227
Mva1269I GAATGC 1 cut(s) 41
MvaI CCWGG 2 cut(s) 605, 1365
MvnI CGCG 2 cut(s) 146, 1159
MwoI GCNNNNNNNGC 2 cut(s) 1511, 1595
NciI CCSGG 1 cut(s) 560
NcoI CCATGG 1 cut(s) 589
NdeII GATC 9 cut(s) 169, 439, 508, 967, 1030, 1069, 1130, 1154, 1448
NlaIV GGNNCC 3 cut(s) 969, 1362, 1534
NmeAIII GCCGAG 1 cut(s) 1525
NmuCI GTSAC 1 cut(s) 113
NspI RCATGY 1 cut(s) 1553
PagI TCATGA 4 cut(s) 934, 1033, 1072, 1465
PceI AGGCCT 1 cut(s) 278
PctI GAATGC 1 cut(s) 41
PdmI GAANNNNTTC 1 cut(s) 851
PfeI GAWTC 1 cut(s) 809
PfoI TCCNGGA 1 cut(s) 603
PkrI GCNGC 9 cut(s) 82, 85, 976, 1204, 1263, 1329, 1513, 1588, 1600
PleI GAGTC 3 cut(s) 263, 1252, 1435
PpsI GAGTC 3 cut(s) 263, 1252, 1435
Ppu21I YACGTR 1 cut(s) 839
Psp124BI GAGCTC 1 cut(s) 504
Psp6I CCWGG 2 cut(s) 603, 1363
PspFI CCCAGC 2 cut(s) 1418, 1540
PspGI CCWGG 2 cut(s) 603, 1363
PspN4I GGNNCC 3 cut(s) 969, 1362, 1534
PspPI GGNCC 4 cut(s) 586, 782, 1478, 1533
PstI CTGCAG 2 cut(s) 976, 1263
PsuI RGATCY 1 cut(s) 967
PvuII CAGCTG 1 cut(s) 1264
RsaI GTAC 4 cut(s) 393, 952, 1017, 1119
RsaNI GTAC 4 cut(s) 392, 951, 1016, 1118
RseI CAYNNNNRTG 4 cut(s) 30, 630, 962, 1095
SacI GAGCTC 1 cut(s) 504
SaqAI TTAA 1 cut(s) 1604
SatI GCNGC 9 cut(s) 81, 84, 975, 1203, 1262, 1328, 1512, 1587, 1599
Sau3AI GATC 9 cut(s) 169, 439, 508, 967, 1030, 1069, 1130, 1154, 1448
Sau96I GGNCC 4 cut(s) 586, 782, 1478, 1533
ScaI AGTACT 1 cut(s) 393
SchI GAGTC 3 cut(s) 263, 1253, 1436
ScrFI CCNGG 3 cut(s) 560, 605, 1365
SduI GDGCHC 2 cut(s) 504, 1086
SfaNI GCATC 7 cut(s) 74, 254, 603, 637, 966, 1165, 1564
SfcI CTRYAG 2 cut(s) 972, 1259
SinI GGWCC 3 cut(s) 782, 1478, 1533
SmiMI CAYNNNNRTG 4 cut(s) 30, 630, 962, 1095
SmlI CTYRAG 4 cut(s) 572, 945, 1150, 1603
SmoI CTYRAG 4 cut(s) 572, 945, 1150, 1603
Sse9I AATT 7 cut(s) 148, 227, 368, 458, 842, 925, 1314
SseBI AGGCCT 1 cut(s) 278
SsiI CCGC 3 cut(s) 83, 167, 1511
SspMI CTAG 3 cut(s) 516, 1022, 1137
SstI GAGCTC 1 cut(s) 504
StuI AGGCCT 1 cut(s) 278
StyD4I CCNGG 3 cut(s) 558, 603, 1363
StyI CCWWGG 3 cut(s) 273, 589, 1217
TaaI ACNGT 3 cut(s) 50, 164, 1187
TaiI ACGT 2 cut(s) 841, 1255
TaqI TCGA 2 cut(s) 183, 504
TaqII GACCGA 1 cut(s) 920
TasI AATT 7 cut(s) 148, 227, 368, 458, 842, 925, 1314
TatI WGTACW 1 cut(s) 391
TauI GCSGC 2 cut(s) 86, 1514
TfiI GAWTC 1 cut(s) 809
Tru1I TTAA 1 cut(s) 1604
Tru9I TTAA 1 cut(s) 1604
TscAI CASTG 2 cut(s) 37, 75
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 7 cut(s) 80, 974, 1202, 1261, 1327, 1586, 1598
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 9 cut(s) 248, 648, 726, 933, 1079, 1317, 1322, 1335, 1482
TspRI CASTG 2 cut(s) 37, 75
Vha464I CTTAAG 1 cut(s) 1603
VpaK11BI GGWCC 3 cut(s) 782, 1478, 1533
XapI RAATTY 1 cut(s) 148
XbaI TCTAGA 1 cut(s) 1136
XceI RCATGY 1 cut(s) 1553
XmnI GAANNNNTTC 1 cut(s) 851
XspI CTAG 3 cut(s) 516, 1022, 1137
ZrmI AGTACT 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.