Rw2G004780

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009820
Physical Location & Seq
Reverse (-)
4492787 .. 4496021
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G004780.1

Sequence Viewer

Length: 1854 bp
ATGCTCGACGGACTGCTTGGCCGTGGATTCGGCGCCAAGTGTAAATCGCTGATAAAATTGACGAAAACTCGGATCGATGTGATTCGGAGGAAGAGGAACGCGACGCAGAAGTTTTTGAAGAAAGATATAGCTGATCTACTCAACCATGGCCTCGAAATTAATGCTTTCGGAAGGGCTGAGGGACTTACAGTGGAGGTGATACTTTCGGCGTGTTACGATTTCGTTGAGCGATGCTGTGATTTGGTGTTGAAGCATCTTTCAGTCATGCAGAAACAAAGTGAATGCCCTGAGGACTGTAAGGAAGCTGTTGCATCACTGATGGTTGCTGCGGCAAGATTTTCTGATTTACCAGAGTTGCGTGACATTAGGCAACTATTTCAAGAGAGATACGCGAATTCTTTGGATTACTGTGCGGATCAAAAGTTTGTTGAGAACATAGCTTCCAAGCCTCCCACATTGGAAAAGAAGGTTCAATTGATGAAAGACATAGCATCAGAGTATTCAATAAAGTGGGACTCCAAGGCCTTTGAACAAATGATGTCTAAACCTCCTGCTTTTATACAGGACAAGCCAAAAACTTATGGGTCTTTCCCTGTTACTGACGATAAAGGCAAATTGTCTAATGGGAATGGTGCAGTACTAAAAGGAGATAGGCATGACCATCGGTCAACAGAAAGGAGACTTGATCCTAATGGTGGCCACAAATTACAGAACAACAAGGAGGTTATAGCCTTGAAGAAGGATGAGCTCGATGATCAGACTAGGCATAGACCTTTAGGAAAAGAATACAAGTCTCTGAAAGGCCGGGAAGGTAGTCTCAAGGAAAAAGAGGGCCATGGCATCTTGTTCCAGGAAAGGCAAGAAGTTGCTCACAGGTATGAAGTTGCTCCTTCAAAACCAGTTAGATTAGGCAGTTCTTCTCGGGGAAAAGGAGCAGAAAGACATTATGAAGCAGAGAATAGTGTTCGTAAAAGGGACGACCCCGACACTATTCCTCATGTAAAGCCAAATGTTGCCGGACCACCTGTGAAAAGTGATGGTAAAGATTCTTTTGCTGGTAAAGAAGAAGGAACACCTAAATTGAAGCCCTTCTCCAACTATGGCCTTCCTCCTCCTTATGTGAAATCTAATGTCAGAGCAAAAGATAGAGAACATGAAGCTAATTTCGGTCATGATTGTTCTCAAGGTACTGGCATCTCTATGGATCCTGCAGCATATAAGAGAGCCTTTGCTGATAATGTGTTGGAAAGGGTACAACTAGGTCGTGATCATGAGAGGCGTGCTACTGCAAAAGTTGATAGTTATGATCATGAGAAGGGGCACGTTCATCAGGATGATATAGCTACCAACAATGTACCAAAACCAAGATCATCTAGAAGGAGACCCTCAAGATCGCGCTCCTCTACCAATGATGCTGGCAACGGTGAAGATATAGGAGCTGCAATGAGCAAACCAAGAAGCAAGAGTAGGAGAAGGGATGAGTCAAGACGTGGCCTGCAGCTGTTGTTTGATGATGACCACCACCACAGGAAAGATGATGAAGAGAGAAGAATTGACGAACTGCTGCTTCATTACAGCACAAAGCCATCTAACTTGGAGCCAGGGCAGTCAAGAAGAAAGTCAAAAAGTCGTCATGCAAGCAGAGATAAGCCTGCTGGGGAGGAGTCTGAACCAACTCATGTAAGATCAACTTCCCTTCCTCATGAACATTCAGGTCCATCAGAGACGCCCAAAGTATTTGCTCGTGCGGCTTCCTTCCAACCAGATGGGTCCAACCCAGCTCGGCATGTGCATCCCAAGTTGCCCGACTATGAGGATTTGGCTGCCAAGTTTGCAGCCTTAAGAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

617

Amino Acids

69.45

Weight (kDa)

9.36

Isoelectric Point (pI)

47.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 170 4.6e-43 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 32
AccII CGCG 3 cut(s) 101, 392, 1396
AciI CCGC 3 cut(s) 329, 413, 1748
AclWI GGATC 5 cut(s) 80, 423, 680, 1199, 1212
AcoI YGGCCR 2 cut(s) 19, 697
AcsI RAATTY 1 cut(s) 394
AcyI GRCGYC 2 cut(s) 33, 1727
AfaI GTAC 4 cut(s) 639, 1189, 1254, 1356
AfiI CCNNNNNNNGG 1 cut(s) 695
AflII CTTAAG 1 cut(s) 1840
AgsI TTSAA 9 cut(s) 118, 250, 380, 473, 504, 530, 736, 894, 1084
AhdI GACNNNNNGTC 1 cut(s) 664
AjiI CACGTC 1 cut(s) 1490
AjnI CCWGG 2 cut(s) 849, 1600
AloI GAACNNNNNNTCC 2 cut(s) 425, 457
AluBI AGCT 9 cut(s) 131, 305, 440, 748, 1160, 1343, 1439, 1501, 1781
AluI AGCT 9 cut(s) 131, 305, 440, 748, 1160, 1343, 1439, 1501, 1781
Alw21I GWGCWC 1 cut(s) 750
Alw26I GTCTC 5 cut(s) 673, 798, 821, 1375, 1718
AlwI GGATC 5 cut(s) 80, 423, 680, 1199, 1212
Ama87I CYCGRG 1 cut(s) 921
AoxI GGCC 8 cut(s) 19, 148, 522, 697, 802, 832, 1102, 1492
ApeKI GCWGC 7 cut(s) 326, 1211, 1439, 1498, 1564, 1823, 1835
ApoI RAATTY 1 cut(s) 394
AseI ATTAAT 1 cut(s) 159
Asp700I GAANNNNTTC 1 cut(s) 1088
AspLEI GCGC 2 cut(s) 35, 1398
AspS9I GGNCC 4 cut(s) 832, 1019, 1715, 1770
AsuC2I CCSGG 1 cut(s) 806
AsuHPI GGTGA 2 cut(s) 208, 1436
AvaI CYCGRG 1 cut(s) 921
AvaII GGWCC 3 cut(s) 1019, 1715, 1770
AxyI CCTNAGG 1 cut(s) 288
BaeGI GKGCMC 1 cut(s) 1323
BalI TGGCCA 1 cut(s) 699
BamHI GGATCC 1 cut(s) 1204
BanI GGYRCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 750
BauI CACGAG 1 cut(s) 1743
Bbv12I GWGCWC 1 cut(s) 750
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 7 cut(s) 313, 1223, 1426, 1510, 1551, 1810, 1847
BccI CCATC 6 cut(s) 313, 669, 1031, 1594, 1726, 1760
BceAI ACGGC 1 cut(s) 6
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 644, 678
BciT130I CCWGG 2 cut(s) 851, 1602
BclI TGATCA 3 cut(s) 754, 1267, 1306
BcnI CCSGG 1 cut(s) 806
BcoDI GTCTC 5 cut(s) 673, 798, 821, 1375, 1718
BfaI CTAG 3 cut(s) 762, 1259, 1374
BfmI CTRYAG 2 cut(s) 1209, 1496
BfoI RGCGCY 1 cut(s) 36
BfrI CTTAAG 1 cut(s) 1840
BisI GCNGC 9 cut(s) 327, 330, 1212, 1440, 1499, 1565, 1749, 1824, 1836
BlsI GCNGC 9 cut(s) 328, 331, 1213, 1441, 1500, 1566, 1750, 1825, 1837
BmcAI AGTACT 1 cut(s) 639
Bme1390I CCNGG 3 cut(s) 806, 851, 1602
Bme18I GGWCC 3 cut(s) 1019, 1715, 1770
BmeRI GACNNNNNGTC 1 cut(s) 664
BmeT110I CYCGRG 1 cut(s) 921
BmgBI CACGTC 1 cut(s) 1490
BmgT120I GGNCC 4 cut(s) 832, 1019, 1715, 1770
BmiI GGNNCC 4 cut(s) 34, 1206, 1599, 1771
BmrFI CCNGG 3 cut(s) 806, 851, 1602
BmsI GCATC 8 cut(s) 221, 262, 320, 500, 849, 1203, 1402, 1801
Bpu10I CCTNAGC 1 cut(s) 177
BpuEI CTTGAG 3 cut(s) 803, 1167, 1372
BpuMI CCSGG 1 cut(s) 806
Bsa29I ATCGAT 1 cut(s) 75
BsaHI GRCGYC 2 cut(s) 33, 1727
BsaI GGTCTC 1 cut(s) 1375
BsaJI CCNNGG 5 cut(s) 22, 145, 519, 835, 1601
Bsc4I CCNNNNNNNGG 1 cut(s) 695
Bse1I ACTGG 2 cut(s) 899, 1195
Bse21I CCTNAGG 1 cut(s) 288
Bse3DI GCAATG 1 cut(s) 1449
BseBI CCWGG 2 cut(s) 851, 1602
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 5 cut(s) 22, 145, 519, 835, 1601
BseGI GGATG 4 cut(s) 748, 1339, 1483, 1792
BseLI CCNNNNNNNGG 1 cut(s) 695
BseMI GCAATG 1 cut(s) 1449
BseMII CTCAG 2 cut(s) 168, 279
BseNI ACTGG 2 cut(s) 899, 1195
BseRI GAGGAG 3 cut(s) 1101, 1390, 1676
BseSI GKGCMC 1 cut(s) 1323
BseXI GCAGC 7 cut(s) 313, 1223, 1426, 1510, 1551, 1810, 1847
BseYI CCCAGC 2 cut(s) 1655, 1777
BsgI GTGCAG 1 cut(s) 654
Bsh1236I CGCG 3 cut(s) 101, 392, 1396
BshFI GGCC 8 cut(s) 21, 150, 524, 699, 804, 834, 1104, 1494
BshNI GGYRCC 1 cut(s) 32
BshVI ATCGAT 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 750
BsiHKCI CYCGRG 1 cut(s) 921
BsiSI CCGG 2 cut(s) 805, 1017
BslFI GGGAC 3 cut(s) 195, 527, 989
BslI CCNNNNNNNGG 1 cut(s) 695
BsmAI GTCTC 5 cut(s) 673, 798, 821, 1375, 1718
BsmBI CGTCTC 1 cut(s) 1718
BsmFI GGGAC 3 cut(s) 195, 527, 989
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 8 cut(s) 21, 150, 524, 699, 804, 834, 1104, 1494
Bso31I GGTCTC 1 cut(s) 1375
BsoBI CYCGRG 1 cut(s) 921
Bsp1286I GDGCHC 2 cut(s) 750, 1323
Bsp19I CCATGG 2 cut(s) 145, 835
BspACI CCGC 3 cut(s) 329, 413, 1748
BspANI GGCC 8 cut(s) 21, 150, 524, 699, 804, 834, 1104, 1494
BspCNI CTCAG 2 cut(s) 169, 280
BspDI ATCGAT 1 cut(s) 75
BspFNI CGCG 3 cut(s) 101, 392, 1396
BspHI TCATGA 4 cut(s) 1171, 1270, 1309, 1702
BspLI GGNNCC 4 cut(s) 34, 1206, 1599, 1771
BspMAI CTGCAG 2 cut(s) 1213, 1500
BspPI GGATC 5 cut(s) 80, 423, 680, 1199, 1212
BspT107I GGYRCC 1 cut(s) 32
BspTI CTTAAG 1 cut(s) 1840
BspTNI GGTCTC 1 cut(s) 1375
BsrDI GCAATG 1 cut(s) 1449
BsrI ACTGG 2 cut(s) 899, 1195
BssECI CCNNGG 5 cut(s) 22, 145, 519, 835, 1601
BssNI GRCGYC 2 cut(s) 33, 1727
BssSI CACGAG 1 cut(s) 1743
BssT1I CCWWGG 3 cut(s) 145, 519, 835
Bst2BI CACGAG 1 cut(s) 1743
Bst2UI CCWGG 2 cut(s) 851, 1602
Bst4CI ACNGT 4 cut(s) 190, 296, 410, 1424
Bst6I CTCTTC 2 cut(s) 86, 1536
BstACI GRCGYC 2 cut(s) 33, 1727
BstAFI CTTAAG 1 cut(s) 1840
BstC8I GCNNGC 5 cut(s) 1281, 1417, 1496, 1639, 1653
BstDEI CTNAG 2 cut(s) 177, 288
BstDSI CCRYGG 3 cut(s) 22, 145, 835
BstF5I GGATG 4 cut(s) 748, 1339, 1483, 1792
BstFNI CGCG 3 cut(s) 101, 392, 1396
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 1398
BstMAI GTCTC 5 cut(s) 673, 798, 821, 1375, 1718
BstMWI GCNNNNNNNGC 2 cut(s) 1748, 1832
BstNI CCWGG 2 cut(s) 851, 1602
BstNSI RCATGY 1 cut(s) 1790
BstSCI CCNGG 3 cut(s) 804, 849, 1600
BstSFI CTRYAG 2 cut(s) 1209, 1496
BstSLI GKGCMC 1 cut(s) 1323
BstUI CGCG 3 cut(s) 101, 392, 1396
BstV1I GCAGC 7 cut(s) 313, 1223, 1426, 1510, 1551, 1810, 1847
BstX2I RGATCY 1 cut(s) 1204
BstXI CCANNNNNNTGG 1 cut(s) 1766
BstYI RGATCY 1 cut(s) 1204
Bsu15I ATCGAT 1 cut(s) 75
Bsu36I CCTNAGG 1 cut(s) 288
BsuRI GGCC 8 cut(s) 21, 150, 524, 699, 804, 834, 1104, 1494
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 3 cut(s) 22, 145, 835
BtgZI GCGATG 1 cut(s) 244
BtrI CACGTC 1 cut(s) 1490
BtsCI GGATG 4 cut(s) 748, 1339, 1483, 1792
BtsIMutI CAGTG 2 cut(s) 195, 314
Cac8I GCNNGC 5 cut(s) 1281, 1417, 1496, 1639, 1653
CciI TCATGA 4 cut(s) 1171, 1270, 1309, 1702
CfoI GCGC 2 cut(s) 35, 1398
Cfr13I GGNCC 4 cut(s) 832, 1019, 1715, 1770
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 2 cut(s) 112, 1735
Csp6I GTAC 4 cut(s) 638, 1188, 1253, 1355
CviQI GTAC 4 cut(s) 638, 1188, 1253, 1355
DdeI CTNAG 2 cut(s) 177, 288
DinI GGCGCC 1 cut(s) 34
DriI GACNNNNNGTC 1 cut(s) 664
EaeI YGGCCR 2 cut(s) 19, 697
Eam1104I CTCTTC 2 cut(s) 86, 1536
Eam1105I GACNNNNNGTC 1 cut(s) 664
EarI CTCTTC 2 cut(s) 86, 1536
Ecl136II GAGCTC 1 cut(s) 748
Eco130I CCWWGG 3 cut(s) 145, 519, 835
Eco147I AGGCCT 1 cut(s) 524
Eco24I GRGCYC 1 cut(s) 750
Eco31I GGTCTC 1 cut(s) 1375
Eco47I GGWCC 3 cut(s) 1019, 1715, 1770
Eco53kI GAGCTC 1 cut(s) 748
Eco81I CCTNAGG 1 cut(s) 288
Eco88I CYCGRG 1 cut(s) 921
EcoICRI GAGCTC 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 849, 1600
EcoT14I CCWWGG 3 cut(s) 145, 519, 835
EcoT38I GRGCYC 1 cut(s) 750
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 3 cut(s) 145, 519, 835
Esp3I CGTCTC 1 cut(s) 1718
FalI AAGNNNNNCTT 4 cut(s) 1211, 1243, 1675, 1707
FaqI GGGAC 3 cut(s) 195, 527, 989
FbaI TGATCA 3 cut(s) 754, 1267, 1306
Fnu4HI GCNGC 9 cut(s) 327, 330, 1212, 1440, 1499, 1565, 1749, 1824, 1836
FokI GGATG 4 cut(s) 755, 1346, 1490, 1779
FriOI GRGCYC 1 cut(s) 750
Fsp4HI GCNGC 9 cut(s) 327, 330, 1212, 1440, 1499, 1565, 1749, 1824, 1836
FspBI CTAG 3 cut(s) 762, 1259, 1374
GlaI GCGC 2 cut(s) 34, 1397
GluI GCNGC 9 cut(s) 327, 330, 1212, 1440, 1499, 1565, 1749, 1824, 1836
GsaI CCCAGC 2 cut(s) 1659, 1781
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 8 cut(s) 21, 150, 524, 699, 804, 834, 1104, 1494
HapII CCGG 2 cut(s) 805, 1017
HgaI GACGC 2 cut(s) 112, 1735
HhaI GCGC 2 cut(s) 35, 1398
Hin1I GRCGYC 2 cut(s) 33, 1727
Hin6I GCGC 2 cut(s) 33, 1396
HinP1I GCGC 2 cut(s) 33, 1396
HincII GTYRAC 1 cut(s) 669
HindII GTYRAC 1 cut(s) 669
HinfI GANTC 6 cut(s) 27, 82, 515, 1046, 1481, 1664
HpaII CCGG 2 cut(s) 805, 1017
HphI GGTGA 2 cut(s) 208, 1436
Hpy166II GTNNAC 1 cut(s) 669
Hpy8I GTNNAC 1 cut(s) 669
Hpy99I CGWCG 2 cut(s) 11, 106
HpyCH4III ACNGT 4 cut(s) 190, 296, 410, 1424
HpyCH4IV ACGT 2 cut(s) 1323, 1489
HpyF10VI GCNNNNNNNGC 2 cut(s) 1748, 1832
HpyF3I CTNAG 2 cut(s) 177, 288
HpySE526I ACGT 2 cut(s) 1323, 1489
Hsp92I GRCGYC 2 cut(s) 33, 1727
HspAI GCGC 2 cut(s) 33, 1396
KasI GGCGCC 1 cut(s) 32
Ksp22I TGATCA 3 cut(s) 754, 1267, 1306
LmnI GCTCC 5 cut(s) 892, 932, 1403, 1436, 1597
Lsp1109I GCAGC 7 cut(s) 313, 1223, 1426, 1510, 1551, 1810, 1847
LweI GCATC 8 cut(s) 221, 262, 320, 500, 849, 1203, 1402, 1801
MaeI CTAG 3 cut(s) 762, 1259, 1374
MaeII ACGT 2 cut(s) 1323, 1489
MaeIII GTNAC 3 cut(s) 212, 359, 595
MboII GAAGA 9 cut(s) 103, 130, 748, 909, 1076, 1439, 1553, 1560, 1626
MfeI CAATTG 1 cut(s) 473
MflI RGATCY 1 cut(s) 1204
MhlI GDGCHC 2 cut(s) 750, 1323
MlsI TGGCCA 1 cut(s) 699
MluCI AATT 9 cut(s) 56, 156, 394, 473, 614, 704, 1079, 1162, 1551
MluNI TGGCCA 1 cut(s) 699
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 3 cut(s) 509, 1490, 1673
MmeI TCCRAC 4 cut(s) 1119, 1224, 1783, 1797
Mox20I TGGCCA 1 cut(s) 699
MroXI GAANNNNTTC 1 cut(s) 1088
MscI TGGCCA 1 cut(s) 699
MseI TTAA 2 cut(s) 159, 1841
MslI CAYNNNNRTG 3 cut(s) 876, 1199, 1332
Msp20I TGGCCA 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 1501
MspCI CTTAAG 1 cut(s) 1840
MspI CCGG 2 cut(s) 805, 1017
MspR9I CCNGG 3 cut(s) 806, 851, 1602
MunI CAATTG 1 cut(s) 473
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 2 cut(s) 851, 1602
MvnI CGCG 3 cut(s) 101, 392, 1396
MwoI GCNNNNNNNGC 2 cut(s) 1748, 1832
NarI GGCGCC 1 cut(s) 33
NciI CCSGG 1 cut(s) 806
NcoI CCATGG 2 cut(s) 145, 835
NlaIV GGNNCC 4 cut(s) 34, 1206, 1599, 1771
NmeAIII GCCGAG 1 cut(s) 1762
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 1 cut(s) 1790
PagI TCATGA 4 cut(s) 1171, 1270, 1309, 1702
PceI AGGCCT 1 cut(s) 524
PctI GAATGC 1 cut(s) 287
PdmI GAANNNNTTC 1 cut(s) 1088
PfeI GAWTC 3 cut(s) 27, 82, 1046
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 9 cut(s) 328, 331, 1213, 1441, 1500, 1566, 1750, 1825, 1837
PleI GAGTC 3 cut(s) 509, 1489, 1672
PluTI GGCGCC 1 cut(s) 36
PpsI GAGTC 3 cut(s) 509, 1489, 1672
PshBI ATTAAT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 750
Psp6I CCWGG 2 cut(s) 849, 1600
PspFI CCCAGC 2 cut(s) 1655, 1777
PspGI CCWGG 2 cut(s) 849, 1600
PspN4I GGNNCC 4 cut(s) 34, 1206, 1599, 1771
PspPI GGNCC 4 cut(s) 832, 1019, 1715, 1770
PstI CTGCAG 2 cut(s) 1213, 1500
PsuI RGATCY 1 cut(s) 1204
PvuII CAGCTG 1 cut(s) 1501
RsaI GTAC 4 cut(s) 639, 1189, 1254, 1356
RsaNI GTAC 4 cut(s) 638, 1188, 1253, 1355
RseI CAYNNNNRTG 3 cut(s) 876, 1199, 1332
SacI GAGCTC 1 cut(s) 750
SaqAI TTAA 2 cut(s) 159, 1841
SatI GCNGC 9 cut(s) 327, 330, 1212, 1440, 1499, 1565, 1749, 1824, 1836
Sau96I GGNCC 4 cut(s) 832, 1019, 1715, 1770
ScaI AGTACT 1 cut(s) 639
SchI GAGTC 3 cut(s) 509, 1490, 1673
ScrFI CCNGG 3 cut(s) 806, 851, 1602
SduI GDGCHC 2 cut(s) 750, 1323
SfaNI GCATC 8 cut(s) 221, 262, 320, 500, 849, 1203, 1402, 1801
SfcI CTRYAG 2 cut(s) 1209, 1496
SfoI GGCGCC 1 cut(s) 34
SinI GGWCC 3 cut(s) 1019, 1715, 1770
SmiMI CAYNNNNRTG 3 cut(s) 876, 1199, 1332
SmlI CTYRAG 4 cut(s) 818, 1182, 1387, 1840
SmoI CTYRAG 4 cut(s) 818, 1182, 1387, 1840
Sse9I AATT 9 cut(s) 56, 156, 394, 473, 614, 704, 1079, 1162, 1551
SseBI AGGCCT 1 cut(s) 524
SsiI CCGC 3 cut(s) 329, 413, 1748
SspDI GGCGCC 1 cut(s) 32
SspMI CTAG 3 cut(s) 762, 1259, 1374
SstI GAGCTC 1 cut(s) 750
StuI AGGCCT 1 cut(s) 524
StyD4I CCNGG 3 cut(s) 804, 849, 1600
StyI CCWWGG 3 cut(s) 145, 519, 835
TaaI ACNGT 4 cut(s) 190, 296, 410, 1424
TaiI ACGT 2 cut(s) 1326, 1492
TaqI TCGA 4 cut(s) 6, 75, 153, 750
TaqII GACCGA 2 cut(s) 654, 1157
TasI AATT 9 cut(s) 56, 156, 394, 473, 614, 704, 1079, 1162, 1551
TatI WGTACW 1 cut(s) 637
TauI GCSGC 2 cut(s) 332, 1751
TfiI GAWTC 3 cut(s) 27, 82, 1046
Tru1I TTAA 2 cut(s) 159, 1841
Tru9I TTAA 2 cut(s) 159, 1841
TscAI CASTG 2 cut(s) 195, 321
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 7 cut(s) 326, 1211, 1439, 1498, 1564, 1823, 1835
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 8 cut(s) 494, 894, 963, 1170, 1316, 1554, 1559, 1719
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 2 cut(s) 195, 321
Vha464I CTTAAG 1 cut(s) 1840
VpaK11BI GGWCC 3 cut(s) 1019, 1715, 1770
VspI ATTAAT 1 cut(s) 159
XapI RAATTY 1 cut(s) 394
XbaI TCTAGA 1 cut(s) 1373
XceI RCATGY 1 cut(s) 1790
XmnI GAANNNNTTC 1 cut(s) 1088
XspI CTAG 3 cut(s) 762, 1259, 1374
ZrmI AGTACT 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.