MD02G1047800.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009820
Physical Location & Seq
Forward (+)
3783609 .. 3787529
3921 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1047800.v1.1.491

Sequence Viewer

Length: 2097 bp
ATGTTGGACGGGCTGCTCGGCCGTGGATTTGCCTCCAAATGCAAAACGCTGATTAAATTGACGAAAACTCGGATCGATGTGGTACGGAGGAAGAGGAACGCGACGCAGAAGTTTCTGAAGAAAGATATCGCTGATCTGCTCACCAATGGCCTTGATATCAATGCTTACGGAAGGGCTGATGGACTCACCGCGGAGGTGATCCTTTCGTCGTGTTATGATTTCGTCGAGCGTTGCTGTGATTTGGTGCTCAAGAATCTTTCAGTTATGCAGAAACAAAGTGAATGCCCGGAGGAATGTAAGGAACCTGTGTCATCGCTGATGATTGCTGCCGCAAGGTTCTCTGATTTACCAGAGTTGCGTGACCTTAGGCAACTATTTCAAGAGAGATACGCGAATGCTCTGGAGTTATTTGTGAATCAAAAGTTTGTTGAGAACATAGCTTCAAAGCCTCCTACACTGGAGAAGAAGGTGCAGTTAATGAAAGACATAGCAGTAGAGTTCTCAATAAATTGGGACTCCAAGGCCTTTGTACAGAGGATGTCGAAACCTCCTCCTGCAATTACACAGGACAAGCCAAAATTTTACGGGCCTTCCCATGCTAGTGATGATAAAGCCAAATTGTCTACCGGGAAATCTGCTGTTACAAAAGGAGATCAGCATAATCTTTTTCCAACAGAAAAGAGACTTGATTTTAATGATGCTCAGAAATTACACAAGGAGAAGGATGGTGTTGTCTTGAAAAAGGAGGAGCACGTTCATCAAACTAGGCATAAAGCCTTTAGAATAGAACACAAGTCTCTCAGTGCCAGGGAAGATACTGTGGAAAAAGATGGCCTTGGAATTTTATTCGGGGAAAGGCAAGAAGTGGCTTCTCACAAAAATGAAGCATGGAATAGAAAGGAGGACGATCACATTCATCAGACTAGTCATAGACCCTTTGGAAAAGAACACAAATCTCTTAGTGCCAGGGACGATACTGTCATGGAAAGAGATGGCCACAGAAGTTTGTTCCAGGAAAGGCAAGAAGTTGCTTCTCACAAATATGAAGCTTGGAATAGAAAGGAGGATGCTCCCCCGAAGTCAGTCAGATCGGGCAGTTCATCTCAAGGAGATGGACAGGAAAAGCATCGTGAGAGGGAGAACAATATTCCTAAAAGAGAGCATGAAGCTTTACCTCGTGTACAGCCAAGTGTTGCTGGACCTCATGTGAAAAGCAACGGTCAAGGCTTATTTGCTGGTGATAATTATGGTGGCCAACACAGTGACGCAAAATTAGCAATTAAAGAAGAAGAGGCGCCGAAGTTGAAGCCCCCCTACAATTATGGGGTCCCTCCTCCGTATGTGAAACCGAATGCCAAAGCAAAAGATCGAAAACATGAAGCCAAGGATGGTTCTGGCAGTAAGCACATCACTAAGGATCCTGTAGCGTACAACATAGCCTTTGTTGACAATATACTCAGAGGGGTAAACGTAGGATCTGATGACGAGGATGAGATGCCTGCTGCTGCAAAACTTTATAAGCATGATCATGAGAAGGATCGTGCTCATCGGGATGATGCATCTAGCAACCACATACCAAAACCAAGATCTTCCAGGACAAGACGCTCAAGATCACGGTCCTCTCACAATGATGCTGGAAACAATGAAGATGCAGAAGTTGCAATCAGAAAAACAAGAGGCAGGAGAAAGGATGAGTCACGACGAGGTCTGCAACTGTTGTTTGATGACGAGCACTACAAAAAGGATGAAGAAGAGAGGAGAATGGACGAGCTTCTGATTCATTACAGTAAAAAGCCATCCAACGTTGAACCAGAAATGTCAAGAAGAAAGTCGAGGAGTCGTCATGCACATCACGCAGGCACTGGTGTTAAAGAATCCCCAAGACAAGCAAGCAGAGATGAGTCGGAAACGGTTCCTACAGCAGTAAGATCAGTTTCCCTTCCTAATGAACATAGTGGTCCATCAGAGGCACCCAAGGTATTTGCTCGTGCGGCGTCCTTTCAACCCGACAGGTCCAATCCAGCCAAGCATGTGCATCCTAAGCTTCCAGACTACGAAGATTTGGCCGCCCAATTTGCAGCCCTAAGAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

699

Amino Acids

79.06

Weight (kDa)

9.27

Isoelectric Point (pI)

55.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 170 2.2e-42 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1518
AasI GACNNNNNNGTC 1 cut(s) 977
AccB1I GGYRCC 2 cut(s) 1294, 1969
AccI GTMKAC 1 cut(s) 623
AccII CGCG 3 cut(s) 101, 191, 392
AciI CCGC 5 cut(s) 189, 191, 330, 1991, 2067
AclI AACGTT 1 cut(s) 1803
AclWI GGATC 6 cut(s) 80, 193, 1412, 1425, 1483, 1545
AcoI YGGCCR 4 cut(s) 19, 994, 1252, 2064
AcsI RAATTY 2 cut(s) 578, 840
AcuI CTGAAG 1 cut(s) 137
AcyI GRCGYC 2 cut(s) 1295, 1994
AfaI GTAC 4 cut(s) 84, 531, 1182, 1430
AgsI TTSAA 6 cut(s) 380, 444, 739, 1306, 1808, 2003
AhlI ACTAGT 1 cut(s) 923
AjnI CCWGG 4 cut(s) 806, 965, 1011, 1592
AluBI AGCT 5 cut(s) 440, 1049, 1169, 1771, 2044
AluI AGCT 5 cut(s) 440, 1049, 1169, 1771, 2044
Alw21I GWGCWC 4 cut(s) 249, 753, 1546, 1734
Alw26I GTCTC 2 cut(s) 676, 801
AlwI GGATC 6 cut(s) 80, 193, 1412, 1425, 1483, 1545
AlwNI CAGNNNCTG 1 cut(s) 1862
AoxI GGCC 8 cut(s) 19, 148, 522, 587, 832, 994, 1252, 2064
ApeKI GCWGC 5 cut(s) 13, 326, 1502, 1505, 2078
ApoI RAATTY 2 cut(s) 578, 840
Asp700I GAANNNNTTC 1 cut(s) 1911
AspLEI GCGC 1 cut(s) 1297
AspS9I GGNCC 6 cut(s) 587, 1199, 1327, 1617, 1958, 2013
AsuC2I CCSGG 2 cut(s) 287, 628
AsuHPI GGTGA 4 cut(s) 133, 178, 208, 1250
AvaII GGWCC 5 cut(s) 1199, 1327, 1617, 1958, 2013
AxyI CCTNAGG 1 cut(s) 365
BalI TGGCCA 2 cut(s) 996, 1254
BamHI GGATCC 1 cut(s) 1417
BanI GGYRCC 2 cut(s) 1294, 1969
BarI GAAGNNNNNNTAC 2 cut(s) 1298, 1330
BauI CACGAG 2 cut(s) 1176, 1986
Bbv12I GWGCWC 4 cut(s) 249, 753, 1546, 1734
BbvI GCAGC 4 cut(s) 313, 1489, 1492, 2090
BccI CCATC 8 cut(s) 173, 719, 824, 986, 1106, 1382, 1804, 1969
BceAI ACGGC 1 cut(s) 6
BciT130I CCWGG 4 cut(s) 808, 967, 1013, 1594
BclI TGATCA 1 cut(s) 1525
BcnI CCSGG 2 cut(s) 287, 628
BcoDI GTCTC 2 cut(s) 676, 801
BcuI ACTAGT 1 cut(s) 923
BfaI CTAG 4 cut(s) 600, 765, 924, 1563
BfmI CTRYAG 2 cut(s) 1422, 1917
BfoI RGCGCY 1 cut(s) 1298
BglII AGATCT 1 cut(s) 1586
BisI GCNGC 8 cut(s) 14, 327, 330, 1503, 1506, 1992, 2067, 2079
BlsI GCNGC 8 cut(s) 15, 328, 331, 1504, 1507, 1993, 2068, 2080
Bme1390I CCNGG 6 cut(s) 287, 628, 808, 967, 1013, 1594
Bme18I GGWCC 5 cut(s) 1199, 1327, 1617, 1958, 2013
BmgT120I GGNCC 6 cut(s) 587, 1199, 1327, 1617, 1958, 2013
BmiI GGNNCC 7 cut(s) 303, 1296, 1328, 1329, 1419, 1914, 1971
BmrFI CCNGG 6 cut(s) 287, 628, 808, 967, 1013, 1594
BmsI GCATC 9 cut(s) 688, 1057, 1135, 1485, 1546, 1568, 1621, 1639, 2044
BpmI CTGGAG 2 cut(s) 422, 479
Bpu10I CCTNAGC 1 cut(s) 2040
BpuEI CTTGAG 3 cut(s) 233, 1089, 1591
BpuMI CCSGG 2 cut(s) 287, 628
Bsa29I ATCGAT 1 cut(s) 75
BsaHI GRCGYC 2 cut(s) 1295, 1994
BsaJI CCNNGG 8 cut(s) 22, 189, 519, 807, 835, 966, 1383, 1974
Bse1I ACTGG 2 cut(s) 462, 1867
Bse21I CCTNAGG 1 cut(s) 365
BseBI CCWGG 4 cut(s) 808, 967, 1013, 1594
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 8 cut(s) 22, 189, 519, 807, 835, 966, 1383, 1974
BseMII CTCAG 3 cut(s) 716, 814, 1471
BseNI ACTGG 2 cut(s) 462, 1867
BseRI GAGGAG 5 cut(s) 540, 761, 1323, 1771, 1849
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 4 cut(s) 313, 1489, 1492, 2090
BsgI GTGCAG 1 cut(s) 491
Bsh1236I CGCG 3 cut(s) 101, 191, 392
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 8 cut(s) 21, 150, 524, 589, 834, 996, 1254, 2066
BshNI GGYRCC 2 cut(s) 1294, 1969
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 4 cut(s) 249, 753, 1546, 1734
BsiSI CCGG 2 cut(s) 287, 627
BslFI GGGAC 3 cut(s) 527, 983, 1313
BsmAI GTCTC 2 cut(s) 676, 801
BsmFI GGGAC 3 cut(s) 527, 983, 1313
BsmI GAATGC 3 cut(s) 287, 400, 1357
BsnI GGCC 8 cut(s) 21, 150, 524, 589, 834, 996, 1254, 2066
Bsp1286I GDGCHC 4 cut(s) 249, 753, 1546, 1734
Bsp1407I TGTACA 2 cut(s) 529, 1180
BspACI CCGC 5 cut(s) 189, 191, 330, 1991, 2067
BspANI GGCC 8 cut(s) 21, 150, 524, 589, 834, 996, 1254, 2066
BspCNI CTCAG 3 cut(s) 715, 813, 1470
BspDI ATCGAT 1 cut(s) 75
BspFNI CGCG 3 cut(s) 101, 191, 392
BspHI TCATGA 1 cut(s) 1528
BspLI GGNNCC 7 cut(s) 303, 1296, 1328, 1329, 1419, 1914, 1971
BspPI GGATC 6 cut(s) 80, 193, 1412, 1425, 1483, 1545
BspT107I GGYRCC 2 cut(s) 1294, 1969
BsrGI TGTACA 2 cut(s) 529, 1180
BsrI ACTGG 2 cut(s) 462, 1867
BssECI CCNNGG 8 cut(s) 22, 189, 519, 807, 835, 966, 1383, 1974
BssNI GRCGYC 2 cut(s) 1295, 1994
BssSI CACGAG 2 cut(s) 1176, 1986
BssT1I CCWWGG 4 cut(s) 519, 835, 1383, 1974
Bst2BI CACGAG 2 cut(s) 1176, 1986
Bst2UI CCWGG 4 cut(s) 808, 967, 1013, 1594
Bst4CI ACNGT 8 cut(s) 820, 979, 1220, 1262, 1617, 1716, 1787, 1912
Bst6I CTCTTC 3 cut(s) 86, 1284, 1746
BstACI GRCGYC 2 cut(s) 1295, 1994
BstAPI GCANNNNNTGC 1 cut(s) 1658
BstAUI TGTACA 2 cut(s) 529, 1180
BstC8I GCNNGC 3 cut(s) 1500, 1858, 1891
BstDEI CTNAG 8 cut(s) 365, 702, 800, 959, 1413, 1457, 2040, 2084
BstDSI CCRYGG 2 cut(s) 22, 189
BstFNI CGCG 3 cut(s) 101, 191, 392
BstH2I RGCGCY 1 cut(s) 1298
BstHHI GCGC 1 cut(s) 1297
BstMAI GTCTC 2 cut(s) 676, 801
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 6 cut(s) 1274, 1658, 1853, 1991, 2041, 2075
BstNI CCWGG 4 cut(s) 808, 967, 1013, 1594
BstNSI RCATGY 1 cut(s) 2033
BstSCI CCNGG 6 cut(s) 285, 626, 806, 965, 1011, 1592
BstSFI CTRYAG 2 cut(s) 1422, 1917
BstUI CGCG 3 cut(s) 101, 191, 392
BstV1I GCAGC 4 cut(s) 313, 1489, 1492, 2090
BstX2I RGATCY 3 cut(s) 1417, 1475, 1586
BstYI RGATCY 3 cut(s) 1417, 1475, 1586
BstZI CGGCCG 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 75
Bsu36I CCTNAGG 1 cut(s) 365
BsuRI GGCC 8 cut(s) 21, 150, 524, 589, 834, 996, 1254, 2066
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 2 cut(s) 22, 189
BtgZI GCGATG 1 cut(s) 297
BtsIMutI CAGTG 4 cut(s) 455, 808, 1267, 1860
Cac8I GCNNGC 3 cut(s) 1500, 1858, 1891
CaiI CAGNNNCTG 1 cut(s) 1862
CciI TCATGA 1 cut(s) 1528
CfoI GCGC 1 cut(s) 1297
Cfr13I GGNCC 6 cut(s) 587, 1199, 1327, 1617, 1958, 2013
Cfr42I CCGCGG 1 cut(s) 192
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 4 cut(s) 112, 1274, 1611, 1983
Csp6I GTAC 4 cut(s) 83, 530, 1181, 1429
CviQI GTAC 4 cut(s) 83, 530, 1181, 1429
DdeI CTNAG 8 cut(s) 365, 702, 800, 959, 1413, 1457, 2040, 2084
DinI GGCGCC 1 cut(s) 1296
DrdI GACNNNNNNGTC 1 cut(s) 977
DseDI GACNNNNNNGTC 1 cut(s) 977
EaeI YGGCCR 4 cut(s) 19, 994, 1252, 2064
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 3 cut(s) 86, 1284, 1746
EarI CTCTTC 3 cut(s) 86, 1284, 1746
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 4 cut(s) 519, 835, 1383, 1974
Eco147I AGGCCT 1 cut(s) 524
Eco32I GATATC 2 cut(s) 127, 157
Eco47I GGWCC 5 cut(s) 1199, 1327, 1617, 1958, 2013
Eco52I CGGCCG 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 137
Eco81I CCTNAGG 1 cut(s) 365
EcoO109I RGGNCCY 1 cut(s) 1327
EcoRII CCWGG 4 cut(s) 806, 965, 1011, 1592
EcoRV GATATC 2 cut(s) 127, 157
EcoT14I CCWWGG 4 cut(s) 519, 835, 1383, 1974
EcoT22I ATGCAT 1 cut(s) 1561
EgeI GGCGCC 1 cut(s) 1296
EheI GGCGCC 1 cut(s) 1296
ErhI CCWWGG 4 cut(s) 519, 835, 1383, 1974
FalI AAGNNNNNCTT 2 cut(s) 819, 851
FaqI GGGAC 3 cut(s) 527, 983, 1313
FbaI TGATCA 1 cut(s) 1525
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 8 cut(s) 14, 327, 330, 1503, 1506, 1992, 2067, 2079
Fsp4HI GCNGC 8 cut(s) 14, 327, 330, 1503, 1506, 1992, 2067, 2079
FspBI CTAG 4 cut(s) 600, 765, 924, 1563
GlaI GCGC 1 cut(s) 1296
GluI GCNGC 8 cut(s) 14, 327, 330, 1503, 1506, 1992, 2067, 2079
GsuI CTGGAG 2 cut(s) 422, 479
HaeII RGCGCY 1 cut(s) 1298
HaeIII GGCC 8 cut(s) 21, 150, 524, 589, 834, 996, 1254, 2066
HapII CCGG 2 cut(s) 287, 627
HgaI GACGC 4 cut(s) 112, 1274, 1611, 1983
HhaI GCGC 1 cut(s) 1297
Hin1I GRCGYC 2 cut(s) 1295, 1994
Hin6I GCGC 1 cut(s) 1295
HinP1I GCGC 1 cut(s) 1295
HincII GTYRAC 1 cut(s) 1447
HindII GTYRAC 1 cut(s) 1447
HindIII AAGCTT 3 cut(s) 1047, 1167, 2042
HinfI GANTC 9 cut(s) 183, 253, 415, 515, 1694, 1777, 1837, 1874, 1901
HpaII CCGG 2 cut(s) 287, 627
HphI GGTGA 4 cut(s) 133, 178, 208, 1250
Hpy166II GTNNAC 4 cut(s) 624, 1181, 1447, 1468
Hpy8I GTNNAC 4 cut(s) 624, 1181, 1447, 1468
Hpy99I CGWCG 4 cut(s) 106, 211, 227, 1704
HpyAV CCTTC 7 cut(s) 165, 460, 600, 715, 1528, 1949, 2085
HpyCH4III ACNGT 8 cut(s) 820, 979, 1220, 1262, 1617, 1716, 1787, 1912
HpyCH4IV ACGT 3 cut(s) 753, 1470, 1803
HpyF10VI GCNNNNNNNGC 6 cut(s) 1274, 1658, 1853, 1991, 2041, 2075
HpyF3I CTNAG 8 cut(s) 365, 702, 800, 959, 1413, 1457, 2040, 2084
HpySE526I ACGT 3 cut(s) 753, 1470, 1803
Hsp92I GRCGYC 2 cut(s) 1295, 1994
HspAI GCGC 1 cut(s) 1295
KasI GGCGCC 1 cut(s) 1294
KflI GGGWCCC 1 cut(s) 1327
Ksp22I TGATCA 1 cut(s) 1525
KspI CCGCGG 1 cut(s) 192
LmnI GCTCC 2 cut(s) 748, 1075
Lsp1109I GCAGC 4 cut(s) 313, 1489, 1492, 2090
LweI GCATC 9 cut(s) 688, 1057, 1135, 1485, 1546, 1568, 1621, 1639, 2044
MaeI CTAG 4 cut(s) 600, 765, 924, 1563
MaeII ACGT 3 cut(s) 753, 1470, 1803
MaeIII GTNAC 4 cut(s) 359, 640, 1262, 1695
MflI RGATCY 3 cut(s) 1417, 1475, 1586
MhlI GDGCHC 4 cut(s) 249, 753, 1546, 1734
MlsI TGGCCA 2 cut(s) 996, 1254
MluNI TGGCCA 2 cut(s) 996, 1254
Mly113I GGCGCC 1 cut(s) 1295
MlyI GAGTC 5 cut(s) 177, 509, 1703, 1846, 1910
MmeI TCCRAC 3 cut(s) 695, 1824, 1884
Mox20I TGGCCA 2 cut(s) 996, 1254
Mph1103I ATGCAT 1 cut(s) 1561
MroXI GAANNNNTTC 1 cut(s) 1911
MscI TGGCCA 2 cut(s) 996, 1254
MseI TTAA 5 cut(s) 54, 476, 693, 1281, 1869
MslI CAYNNNNRTG 6 cut(s) 600, 879, 1041, 1527, 1551, 1629
Msp20I TGGCCA 2 cut(s) 996, 1254
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 2 cut(s) 287, 627
MspR9I CCNGG 6 cut(s) 287, 628, 808, 967, 1013, 1594
Mva1269I GAATGC 3 cut(s) 287, 400, 1357
MvaI CCWGG 4 cut(s) 808, 967, 1013, 1594
MvnI CGCG 3 cut(s) 101, 191, 392
MwoI GCNNNNNNNGC 6 cut(s) 1274, 1658, 1853, 1991, 2041, 2075
NarI GGCGCC 1 cut(s) 1295
NciI CCSGG 2 cut(s) 287, 628
NlaIV GGNNCC 7 cut(s) 303, 1296, 1328, 1329, 1419, 1914, 1971
NmuCI GTSAC 3 cut(s) 359, 1262, 1695
NsiI ATGCAT 1 cut(s) 1561
NspI RCATGY 1 cut(s) 2033
PagI TCATGA 1 cut(s) 1528
PceI AGGCCT 1 cut(s) 524
PctI GAATGC 3 cut(s) 287, 400, 1357
PdmI GAANNNNTTC 1 cut(s) 1911
PfeI GAWTC 4 cut(s) 253, 415, 1777, 1874
PflFI GACNNNGTC 1 cut(s) 1704
PfoI TCCNGGA 2 cut(s) 1011, 1592
PkrI GCNGC 8 cut(s) 15, 328, 331, 1504, 1507, 1993, 2068, 2080
PleI GAGTC 5 cut(s) 177, 509, 1702, 1845, 1909
PluTI GGCGCC 1 cut(s) 1298
PpsI GAGTC 5 cut(s) 177, 509, 1702, 1845, 1909
PpuMI RGGWCCY 1 cut(s) 1327
PsiI TTATAA 1 cut(s) 1518
Psp1406I AACGTT 1 cut(s) 1803
Psp5II RGGWCCY 1 cut(s) 1327
Psp6I CCWGG 4 cut(s) 806, 965, 1011, 1592
PspGI CCWGG 4 cut(s) 806, 965, 1011, 1592
PspN4I GGNNCC 7 cut(s) 303, 1296, 1328, 1329, 1419, 1914, 1971
PspPI GGNCC 6 cut(s) 587, 1199, 1327, 1617, 1958, 2013
PspPPI RGGWCCY 1 cut(s) 1327
PstNI CAGNNNCTG 1 cut(s) 1862
PsuI RGATCY 3 cut(s) 1417, 1475, 1586
PsyI GACNNNGTC 1 cut(s) 1704
RsaI GTAC 4 cut(s) 84, 531, 1182, 1430
RsaNI GTAC 4 cut(s) 83, 530, 1181, 1429
RseI CAYNNNNRTG 6 cut(s) 600, 879, 1041, 1527, 1551, 1629
SacII CCGCGG 1 cut(s) 192
SaqAI TTAA 5 cut(s) 54, 476, 693, 1281, 1869
SatI GCNGC 8 cut(s) 14, 327, 330, 1503, 1506, 1992, 2067, 2079
Sau96I GGNCC 6 cut(s) 587, 1199, 1327, 1617, 1958, 2013
SchI GAGTC 5 cut(s) 177, 509, 1703, 1846, 1910
ScrFI CCNGG 6 cut(s) 287, 628, 808, 967, 1013, 1594
SduI GDGCHC 4 cut(s) 249, 753, 1546, 1734
SfaNI GCATC 9 cut(s) 688, 1057, 1135, 1485, 1546, 1568, 1621, 1639, 2044
SfcI CTRYAG 2 cut(s) 1422, 1917
SfoI GGCGCC 1 cut(s) 1296
Sfr303I CCGCGG 1 cut(s) 192
SgrBI CCGCGG 1 cut(s) 192
SinI GGWCC 5 cut(s) 1199, 1327, 1617, 1958, 2013
SmiMI CAYNNNNRTG 6 cut(s) 600, 879, 1041, 1527, 1551, 1629
SmlI CTYRAG 3 cut(s) 248, 1104, 1606
SmoI CTYRAG 3 cut(s) 248, 1104, 1606
SpeI ACTAGT 1 cut(s) 923
SseBI AGGCCT 1 cut(s) 524
SsiI CCGC 5 cut(s) 189, 191, 330, 1991, 2067
SspDI GGCGCC 1 cut(s) 1294
SspI AATATT 1 cut(s) 1147
SspMI CTAG 4 cut(s) 600, 765, 924, 1563
StuI AGGCCT 1 cut(s) 524
StyD4I CCNGG 6 cut(s) 285, 626, 806, 965, 1011, 1592
StyI CCWWGG 4 cut(s) 519, 835, 1383, 1974
TaaI ACNGT 8 cut(s) 820, 979, 1220, 1262, 1617, 1716, 1787, 1912
TaiI ACGT 3 cut(s) 756, 1473, 1806
TaqI TCGA 5 cut(s) 75, 225, 542, 1369, 1832
TatI WGTACW 2 cut(s) 529, 1180
TauI GCSGC 3 cut(s) 332, 1994, 2069
TfiI GAWTC 4 cut(s) 253, 415, 1777, 1874
Tru1I TTAA 5 cut(s) 54, 476, 693, 1281, 1869
Tru9I TTAA 5 cut(s) 54, 476, 693, 1281, 1869
TscAI CASTG 4 cut(s) 462, 808, 1267, 1867
TseFI GTSAC 3 cut(s) 359, 1262, 1695
TseI GCWGC 5 cut(s) 13, 326, 1502, 1505, 2078
Tsp45I GTSAC 3 cut(s) 359, 1262, 1695
TspGWI ACGGA 3 cut(s) 100, 183, 1326
TspRI CASTG 4 cut(s) 462, 808, 1267, 1867
Tth111I GACNNNGTC 1 cut(s) 1704
VpaK11BI GGWCC 5 cut(s) 1199, 1327, 1617, 1958, 2013
XapI RAATTY 2 cut(s) 578, 840
XceI RCATGY 1 cut(s) 2033
XmiI GTMKAC 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 1911
XspI CTAG 4 cut(s) 600, 765, 924, 1563
Zsp2I ATGCAT 1 cut(s) 1561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.