FvH4_1g04930

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009820
Physical Location & Seq
Forward (+)
2629279 .. 2633201
3923 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04930.t1

Sequence Viewer

Length: 1863 bp
ATGCTCGACGGACTGCTCGGCCGTGGCTTCGGCGCCAAGTGTAAATCGCTGATTAAATTGACGAAGAGTCGGATCGATGTGATTCGGAGGAAGAGGAACGCGACGCAGAAGTTTCTGAAGAAAGACATAGCTGATCTACTCGCCAATGGCCTCGATATTAATGCTTACGGAAGGGCTGAGGGACTTACAGTGGAGGTGATACTTTCGTCGTGTTACGATTTCGTTGAGAGATGCTGTGATTTGGTGTTGAAGCATCTTTCAGTAATGCAGAAACAGAGGGAATGCCCTGAGGAATGCAAGGAAGCTGTTGCATCACTGATGGTTGCTGCAGCAAGATTTTCTGATTTACCAGAGTTGCGTGACATCAGGCAACTATTTCAAGAGAGATATGCAAATTCTGTGGATTACTGTGTGGATCAAAAGTTCGTTGAGAACATAGCTTCAAAGCCTCCCACATTGGAGAAGAAGGTTCAGTTGATGAAAGACATAGCATCAGAGTTTTCAATAAAGTGGGACTCCTGGGCCTTTGAACAAAGGATGTCTAAACCTCCTGCATTTATACAGGACAAGCCAAAAACTTATGGGTCTTTCCCTGTTACTGATGATAAAGACAAATTGTCTAATGGAAAAGGTGCAGTACCAAAAGGAGATAGGCATGACCATGTGGCAACAGAAAGGAGACTTGATCCTAATGGTGGCCAAAAATTACAGAACCTCAAGGAACTTATTGCCTCGAATAATGATGAGCTCGATGATCAGACTAGCCATAGACATTTAGGTAGAGATTCCAAGTCTCTCAAAGGCCGGGAAGGTAGTCTCAAGGAAAAAGAGGGCCATGGCATCTTATTCCAGGAAAGGCAAGAAGTTGCACAAAAGTATGAGGCAAGAAGAGAGGATGCTCCCCCAAAACCAGTTAGATTAGGCAGTTCTTCTCGGGGAAAAGGACTGGAAAGGCATTATGAAGCGGAAAATTTTGTTCCTAAAAGAGACGGCCCTGATATTCCACCTCATGTAAAGCCAAATGTTGCTGGACCACCTGTGATAAGTAATGGTAAAGATTCATTTGCTGGTAGAGAAGAAGGAACGCCTAAATTGAAGCCCTTCTCCAACTATGGCCTTCCTCCTCCTTATGTGAAATCTAATGTCAGAGCAAAAGACAGAAAACATGAAGCCAGTTTGGGTCATGATAATTCTCAAGATACGGGCATCTCTACCGATCCTACGGCATATAAGAGAGCCTTTGCTGAAAATGTGTTGGAAAGGATACAACTTGGTCGTGATCATGACAGGCGTGCCACTGCAAAAGTTGATACTTACGATCATGAGAAGGAGCACATTCATCAGGATGATATAGCTACCAACAGTATACCAAAACCAAGATCATCTAGAAGGAGGCCCTCAAGATCACGTTCCTCTACCAATGATGCTGGCAACAATGAAGATACAGGAGTTGTGATGAGCAAACCAAGAAGCAGGAGTAGGAGAAGGGATGACTCAAGAAGAGGCCTACAGCTGTTGTTTGATGATGACCACCACCACCGGAAAGATGATGAAGAGAGAAGAATTGATGAACTGCTGCTTCATTACAGCAAAAAGCCATCTAACATAGAGCCAGGGCAATCAAGAAGGAAGTCAAAAAGTCGTCATGCAAGCAGAGATGAGCGTGCTGAGGGGGTGTCTGAACCAACTCATGTAAGATCAAATTCCCTTCCTCATGAACATTCAGGTCCATCAGAGACGCCGAAAGTATTCGCTCGTGCATCTTCCTTCCAACCAGATGGGTCCAACGCTCGGCACGTGCATCCCAAATTACCTGACTATGAGGATTTAGCTGCCAAGTTTGCAGCCTTAAGAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

621

Amino Acids

70.1

Weight (kDa)

9.34

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 169 1.1e-44 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 32
AccI GTMKAC 1 cut(s) 1366
AccII CGCG 1 cut(s) 101
AciI CCGC 1 cut(s) 965
AclWI GGATC 4 cut(s) 80, 423, 680, 1211
AcoI YGGCCR 2 cut(s) 19, 697
AcsI RAATTY 3 cut(s) 394, 970, 1702
AcuI CTGAAG 1 cut(s) 137
AcvI CACGTG 1 cut(s) 1798
AcyI GRCGYC 2 cut(s) 33, 1739
AfaI GTAC 1 cut(s) 639
AfiI CCNNNNNNNGG 2 cut(s) 695, 1791
AflII CTTAAG 1 cut(s) 1849
AgsI TTSAA 6 cut(s) 250, 380, 444, 504, 530, 1096
AhdI GACNNNNNGTC 2 cut(s) 66, 616
AjnI CCWGG 3 cut(s) 518, 849, 1612
AloI GAACNNNNNNTCC 2 cut(s) 407, 439
AluBI AGCT 7 cut(s) 131, 305, 440, 748, 1355, 1513, 1832
AluI AGCT 7 cut(s) 131, 305, 440, 748, 1355, 1513, 1832
Alw21I GWGCWC 2 cut(s) 750, 1335
Alw26I GTCTC 5 cut(s) 673, 798, 821, 981, 1730
AlwI GGATC 4 cut(s) 80, 423, 680, 1211
Ama87I CYCGRG 1 cut(s) 933
ApeKI GCWGC 5 cut(s) 326, 329, 1576, 1832, 1844
ApoI RAATTY 3 cut(s) 394, 970, 1702
AseI ATTAAT 1 cut(s) 159
Asp700I GAANNNNTTC 2 cut(s) 1100, 1748
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 7 cut(s) 522, 832, 992, 1031, 1395, 1727, 1782
AsuC2I CCSGG 1 cut(s) 806
AsuHPI GGTGA 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 933
AvaII GGWCC 3 cut(s) 1031, 1727, 1782
AxyI CCTNAGG 1 cut(s) 288
BaeI ACNNNNGTAYC 4 cut(s) 191, 224, 1434, 1467
BalI TGGCCA 1 cut(s) 699
BanI GGYRCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 750
BauI CACGAG 1 cut(s) 1755
BbrPI CACGTG 1 cut(s) 1798
Bbv12I GWGCWC 2 cut(s) 750, 1335
BbvCI CCTCAGC 2 cut(s) 177, 1668
BbvI GCAGC 5 cut(s) 313, 341, 1563, 1819, 1856
BccI CCATC 4 cut(s) 313, 1606, 1738, 1772
BceAI ACGGC 3 cut(s) 6, 1006, 1239
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 3 cut(s) 520, 851, 1614
BciVI GTATCC 1 cut(s) 1257
BclI TGATCA 2 cut(s) 754, 1279
BcnI CCSGG 1 cut(s) 806
BcoDI GTCTC 5 cut(s) 673, 798, 821, 981, 1730
BfaI CTAG 2 cut(s) 762, 1386
BfmI CTRYAG 2 cut(s) 327, 1508
BfoI RGCGCY 1 cut(s) 36
BfrI CTTAAG 1 cut(s) 1849
BfuI GTATCC 1 cut(s) 1257
BisI GCNGC 5 cut(s) 327, 330, 1577, 1833, 1845
BlsI GCNGC 5 cut(s) 328, 331, 1578, 1834, 1846
Bme1390I CCNGG 4 cut(s) 520, 806, 851, 1614
Bme18I GGWCC 3 cut(s) 1031, 1727, 1782
BmeRI GACNNNNNGTC 2 cut(s) 66, 616
BmeT110I CYCGRG 1 cut(s) 933
BmgT120I GGNCC 7 cut(s) 522, 832, 992, 1031, 1395, 1727, 1782
BmiI GGNNCC 2 cut(s) 34, 1783
BmrFI CCNGG 4 cut(s) 520, 806, 851, 1614
Bpu10I CCTNAGC 2 cut(s) 177, 1668
BpuEI CTTGAG 5 cut(s) 701, 803, 1179, 1384, 1480
BpuMI CCSGG 1 cut(s) 806
Bsa29I ATCGAT 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 1798
BsaHI GRCGYC 2 cut(s) 33, 1739
BsaJI CCNNGG 4 cut(s) 22, 519, 835, 1613
BsaWI WCCGGW 1 cut(s) 1539
Bsc4I CCNNNNNNNGG 2 cut(s) 695, 1791
Bse1I ACTGG 3 cut(s) 911, 951, 1173
Bse21I CCTNAGG 1 cut(s) 288
BseBI CCWGG 3 cut(s) 520, 851, 1614
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 4 cut(s) 22, 519, 835, 1613
BseGI GGATG 5 cut(s) 543, 901, 1351, 1495, 1801
BseLI CCNNNNNNNGG 2 cut(s) 695, 1791
BseMII CTCAG 3 cut(s) 168, 279, 1659
BseNI ACTGG 3 cut(s) 911, 951, 1173
BseRI GAGGAG 1 cut(s) 1113
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 5 cut(s) 313, 341, 1563, 1819, 1856
BsgI GTGCAG 1 cut(s) 654
Bsh1236I CGCG 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 22
BshNI GGYRCC 1 cut(s) 32
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 2 cut(s) 750, 1335
BsiHKCI CYCGRG 1 cut(s) 933
BsiSI CCGG 2 cut(s) 805, 1540
BslFI GGGAC 2 cut(s) 195, 527
BslI CCNNNNNNNGG 2 cut(s) 695, 1791
BsmAI GTCTC 5 cut(s) 673, 798, 821, 981, 1730
BsmBI CGTCTC 2 cut(s) 981, 1730
BsmFI GGGAC 2 cut(s) 195, 527
BsmI GAATGC 2 cut(s) 287, 299
BsoBI CYCGRG 1 cut(s) 933
Bsp1286I GDGCHC 2 cut(s) 750, 1335
Bsp19I CCATGG 1 cut(s) 835
BspACI CCGC 1 cut(s) 965
BspCNI CTCAG 3 cut(s) 169, 280, 1660
BspDI ATCGAT 1 cut(s) 75
BspFNI CGCG 1 cut(s) 101
BspHI TCATGA 4 cut(s) 1183, 1282, 1321, 1714
BspLI GGNNCC 2 cut(s) 34, 1783
BspMAI CTGCAG 1 cut(s) 331
BspPI GGATC 4 cut(s) 80, 423, 680, 1211
BspT107I GGYRCC 1 cut(s) 32
BspTI CTTAAG 1 cut(s) 1849
BsrI ACTGG 3 cut(s) 911, 951, 1173
BssECI CCNNGG 4 cut(s) 22, 519, 835, 1613
BssNAI GTATAC 1 cut(s) 1367
BssNI GRCGYC 2 cut(s) 33, 1739
BssSI CACGAG 1 cut(s) 1755
BssT1I CCWWGG 1 cut(s) 835
Bst1107I GTATAC 1 cut(s) 1367
Bst2BI CACGAG 1 cut(s) 1755
Bst2UI CCWGG 3 cut(s) 520, 851, 1614
Bst4CI ACNGT 3 cut(s) 190, 410, 1364
Bst6I CTCTTC 5 cut(s) 59, 86, 883, 1495, 1548
BstACI GRCGYC 2 cut(s) 33, 1739
BstAFI CTTAAG 1 cut(s) 1849
BstBAI YACGTR 1 cut(s) 1798
BstC8I GCNNGC 4 cut(s) 1293, 1429, 1651, 1665
BstDEI CTNAG 3 cut(s) 177, 288, 1668
BstDSI CCRYGG 2 cut(s) 22, 835
BstF5I GGATG 5 cut(s) 543, 901, 1351, 1495, 1801
BstFNI CGCG 1 cut(s) 101
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 1 cut(s) 35
BstMAI GTCTC 5 cut(s) 673, 798, 821, 981, 1730
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 1841
BstNI CCWGG 3 cut(s) 520, 851, 1614
BstSCI CCNGG 4 cut(s) 518, 804, 849, 1612
BstSFI CTRYAG 2 cut(s) 327, 1508
BstUI CGCG 1 cut(s) 101
BstV1I GCAGC 5 cut(s) 313, 341, 1563, 1819, 1856
BstXI CCANNNNNNTGG 1 cut(s) 1778
BstZ17I GTATAC 1 cut(s) 1367
BstZI CGGCCG 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 75
Bsu36I CCTNAGG 1 cut(s) 288
BsuI GTATCC 1 cut(s) 1257
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 2 cut(s) 22, 835
BtsCI GGATG 5 cut(s) 543, 901, 1351, 1495, 1801
BtsI GCAGTG 1 cut(s) 1296
BtsIMutI CAGTG 3 cut(s) 195, 314, 1296
Cac8I GCNNGC 4 cut(s) 1293, 1429, 1651, 1665
CciI TCATGA 4 cut(s) 1183, 1282, 1321, 1714
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 7 cut(s) 522, 832, 992, 1031, 1395, 1727, 1782
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 2 cut(s) 112, 1747
Csp6I GTAC 1 cut(s) 638
CspCI CAANNNNNGTGG 2 cut(s) 381, 416
CviQI GTAC 1 cut(s) 638
DdeI CTNAG 3 cut(s) 177, 288, 1668
DinI GGCGCC 1 cut(s) 34
DriI GACNNNNNGTC 2 cut(s) 66, 616
EaeI YGGCCR 2 cut(s) 19, 697
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 5 cut(s) 59, 86, 883, 1495, 1548
Eam1105I GACNNNNNGTC 2 cut(s) 66, 616
EarI CTCTTC 5 cut(s) 59, 86, 883, 1495, 1548
Ecl136II GAGCTC 1 cut(s) 748
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 835
Eco147I AGGCCT 1 cut(s) 1506
Eco24I GRGCYC 1 cut(s) 750
Eco47I GGWCC 3 cut(s) 1031, 1727, 1782
Eco52I CGGCCG 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 748
Eco57I CTGAAG 1 cut(s) 137
Eco72I CACGTG 1 cut(s) 1798
Eco81I CCTNAGG 1 cut(s) 288
Eco88I CYCGRG 1 cut(s) 933
EcoICRI GAGCTC 1 cut(s) 748
EcoO109I RGGNCCY 1 cut(s) 1395
EcoRII CCWGG 3 cut(s) 518, 849, 1612
EcoT14I CCWWGG 1 cut(s) 835
EcoT38I GRGCYC 1 cut(s) 750
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 1 cut(s) 835
Esp3I CGTCTC 2 cut(s) 981, 1730
FalI AAGNNNNNCTT 2 cut(s) 1223, 1255
FaqI GGGAC 2 cut(s) 195, 527
FbaI TGATCA 2 cut(s) 754, 1279
FblI GTMKAC 1 cut(s) 1366
Fnu4HI GCNGC 5 cut(s) 327, 330, 1577, 1833, 1845
FokI GGATG 5 cut(s) 550, 908, 1358, 1502, 1788
FriOI GRGCYC 1 cut(s) 750
Fsp4HI GCNGC 5 cut(s) 327, 330, 1577, 1833, 1845
FspBI CTAG 2 cut(s) 762, 1386
GlaI GCGC 1 cut(s) 34
GluI GCNGC 5 cut(s) 327, 330, 1577, 1833, 1845
HaeII RGCGCY 1 cut(s) 36
HapII CCGG 2 cut(s) 805, 1540
HgaI GACGC 2 cut(s) 112, 1747
HhaI GCGC 1 cut(s) 35
Hin1I GRCGYC 2 cut(s) 33, 1739
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HinfI GANTC 6 cut(s) 67, 82, 515, 785, 1058, 1493
HpaII CCGG 2 cut(s) 805, 1540
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 1 cut(s) 1367
Hpy188I TCNGA 9 cut(s) 72, 87, 117, 343, 496, 759, 1148, 1681, 1735
Hpy8I GTNNAC 1 cut(s) 1367
Hpy99I CGWCG 3 cut(s) 11, 106, 211
HpyCH4III ACNGT 3 cut(s) 190, 410, 1364
HpyCH4IV ACGT 2 cut(s) 1408, 1797
HpyF10VI GCNNNNNNNGC 1 cut(s) 1841
HpyF3I CTNAG 3 cut(s) 177, 288, 1668
HpySE526I ACGT 2 cut(s) 1408, 1797
Hsp92I GRCGYC 2 cut(s) 33, 1739
HspAI GCGC 1 cut(s) 33
KasI GGCGCC 1 cut(s) 32
Ksp22I TGATCA 2 cut(s) 754, 1279
LmnI GCTCC 2 cut(s) 904, 1330
Lsp1109I GCAGC 5 cut(s) 313, 341, 1563, 1819, 1856
MaeI CTAG 2 cut(s) 762, 1386
MaeII ACGT 2 cut(s) 1408, 1797
MaeIII GTNAC 3 cut(s) 212, 359, 595
MhlI GDGCHC 2 cut(s) 750, 1335
MlsI TGGCCA 1 cut(s) 699
MluNI TGGCCA 1 cut(s) 699
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 3 cut(s) 76, 509, 1487
MmeI TCCRAC 5 cut(s) 50, 1131, 1236, 1795, 1809
Mox20I TGGCCA 1 cut(s) 699
MroXI GAANNNNTTC 2 cut(s) 1100, 1748
MscI TGGCCA 1 cut(s) 699
MseI TTAA 3 cut(s) 54, 159, 1850
MslI CAYNNNNRTG 2 cut(s) 660, 1344
Msp20I TGGCCA 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 1513
MspCI CTTAAG 1 cut(s) 1849
MspI CCGG 2 cut(s) 805, 1540
MspR9I CCNGG 4 cut(s) 520, 806, 851, 1614
Mva1269I GAATGC 2 cut(s) 287, 299
MvaI CCWGG 3 cut(s) 520, 851, 1614
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 1 cut(s) 1841
NarI GGCGCC 1 cut(s) 33
NciI CCSGG 1 cut(s) 806
NcoI CCATGG 1 cut(s) 835
NlaIV GGNNCC 2 cut(s) 34, 1783
NmeAIII GCCGAG 1 cut(s) 1771
NmuCI GTSAC 1 cut(s) 359
PagI TCATGA 4 cut(s) 1183, 1282, 1321, 1714
PceI AGGCCT 1 cut(s) 1506
PcsI WCGNNNNNNNCGW 1 cut(s) 1794
PctI GAATGC 2 cut(s) 287, 299
PdmI GAANNNNTTC 2 cut(s) 1100, 1748
PfeI GAWTC 3 cut(s) 82, 785, 1058
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 5 cut(s) 328, 331, 1578, 1834, 1846
PleI GAGTC 3 cut(s) 75, 509, 1487
PluTI GGCGCC 1 cut(s) 36
PmaCI CACGTG 1 cut(s) 1798
PmlI CACGTG 1 cut(s) 1798
PpsI GAGTC 3 cut(s) 75, 509, 1487
Ppu21I YACGTR 1 cut(s) 1798
PshBI ATTAAT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 750
Psp6I CCWGG 3 cut(s) 518, 849, 1612
PspCI CACGTG 1 cut(s) 1798
PspGI CCWGG 3 cut(s) 518, 849, 1612
PspN4I GGNNCC 2 cut(s) 34, 1783
PspPI GGNCC 7 cut(s) 522, 832, 992, 1031, 1395, 1727, 1782
PstI CTGCAG 1 cut(s) 331
PvuII CAGCTG 1 cut(s) 1513
RsaI GTAC 1 cut(s) 639
RsaNI GTAC 1 cut(s) 638
RseI CAYNNNNRTG 2 cut(s) 660, 1344
SacI GAGCTC 1 cut(s) 750
SaqAI TTAA 3 cut(s) 54, 159, 1850
SatI GCNGC 5 cut(s) 327, 330, 1577, 1833, 1845
Sau96I GGNCC 7 cut(s) 522, 832, 992, 1031, 1395, 1727, 1782
SchI GAGTC 3 cut(s) 76, 509, 1487
ScrFI CCNGG 4 cut(s) 520, 806, 851, 1614
SduI GDGCHC 2 cut(s) 750, 1335
SfcI CTRYAG 2 cut(s) 327, 1508
SfoI GGCGCC 1 cut(s) 34
SinI GGWCC 3 cut(s) 1031, 1727, 1782
SmiMI CAYNNNNRTG 2 cut(s) 660, 1344
SmlI CTYRAG 6 cut(s) 716, 818, 1194, 1399, 1495, 1849
SmoI CTYRAG 6 cut(s) 716, 818, 1194, 1399, 1495, 1849
SseBI AGGCCT 1 cut(s) 1506
SsiI CCGC 1 cut(s) 965
SspDI GGCGCC 1 cut(s) 32
SspMI CTAG 2 cut(s) 762, 1386
SstI GAGCTC 1 cut(s) 750
StuI AGGCCT 1 cut(s) 1506
StyD4I CCNGG 4 cut(s) 518, 804, 849, 1612
StyI CCWWGG 1 cut(s) 835
TaaI ACNGT 3 cut(s) 190, 410, 1364
TaiI ACGT 2 cut(s) 1411, 1800
TaqI TCGA 5 cut(s) 6, 75, 153, 734, 750
TfiI GAWTC 3 cut(s) 82, 785, 1058
Tru1I TTAA 3 cut(s) 54, 159, 1850
Tru9I TTAA 3 cut(s) 54, 159, 1850
TscAI CASTG 3 cut(s) 195, 321, 1303
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 5 cut(s) 326, 329, 1576, 1832, 1844
Tsp45I GTSAC 1 cut(s) 359
TspGWI ACGGA 2 cut(s) 24, 183
TspRI CASTG 3 cut(s) 195, 321, 1303
Vha464I CTTAAG 1 cut(s) 1849
VpaK11BI GGWCC 3 cut(s) 1031, 1727, 1782
VspI ATTAAT 1 cut(s) 159
XapI RAATTY 3 cut(s) 394, 970, 1702
XbaI TCTAGA 1 cut(s) 1385
XmiI GTMKAC 1 cut(s) 1366
XmnI GAANNNNTTC 2 cut(s) 1100, 1748
XspI CTAG 2 cut(s) 762, 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.