MD15G1186300.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009820
Physical Location & Seq
Forward (+)
14717303 .. 14721407
4105 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1186300.v1.1.491

Sequence Viewer

Length: 2097 bp
ATGTTGGACGGGCTGCTCGGCCGTGGATTTGCCTCCAAATGCAAATCGCTGATTAAATTGACGAAGACTCGGATCGACGTGATACGGAGGAAGAGGAACGCGACGCAGAAGTTTCTGAAGAAGGATATCGCTGATCTGCTCACCAATGGCCTTGATATCAATGCTTTCGGAAGGGCTGATGGACTCACAGCGGAGTTGATCCTTTCTTCGTGTTATGATTTCGTTGAGCGATGCTGTGATTTGGTGCTCAAGAATCTTTCAATTATGCAGAAAGAAAGTGATTGCCCTAAGGAATGTAAGGAGCCTGTGTCATCGCTGATGATTGCTGCCGCAAGGTTCTCTGATTTACCAGAGTTGCGTGACCTTAGGCAACTTTTTCAAGAGAGATACGCAAATTCTCTGGAGTATTTTGTGAATCAAAAGTTTGTTGAGAACATAGCTTCAAAGCCTCCTACACTGGAGAAGAAGATTCAGTTATTGAAAGACATAGCGGTAGAGTTCTCAATAAATTGGGACTCCAAGGCCTTTGAACAGAGGATGGCAAAACCTCCTCCTGCGTTTACACAGGACAAGCCAAAAACTTACGGGCCTTCCCATGTTAGTGACGATAAAAGCAAATTGTCTACTGGGAAATCTGCAGGCCCAAAAGGAGAACATAATCTTTTACCCACAGAAAGGAGATTTGATCTTAATGATGCTCAGAAATTACACAAGGAGAAGCTCGGTATTGTATTGAAAAAAGATGATCATGTTCCTGAAACTAGGCATAGACCCTTTGGAATAGAACATAAGTCTTTCAGTGCCAGGGAAGATACTGTCATGGAAAATGATGGCCATGGAATTTTGTTCCGGGAAAGGCAAGAATTGGCTTCTCACAAATATGAAGCATGGAATAGAAAGGAGGACGATCACGTTCATCGGACTAGGCATAGACCCTTGGGAAAAGAACACAAGTCTCTCAGTGCCAGTGAAGATACTGTCATGGAAATAGATGGTGGAAGTTTGTTCCAGGAAAGGCAAGAAGTTGCTTCCCACAAATATGAAGCTTGGAATAGAAAGGAGGATGCTCCCCCAAAGTCAGTTAGATCAGACAGTTCATCTCAAGGAAAAGGACAGGAAAGGGGTCACGAGAGGGAGAACAATGTTCCTAAAAGAGACTTCCATGAAGTTTTACCTCGTGTACAGCCCAGTGTTGCTGGACCTCATGTGAAAAGCAACGGTCAAGGCTTATTTGCTGATGACAATTATGGTGGCAAACAAAATGACGCAAAATTAGGAATTAAAGAAGAAGAGATGCCTAAGTTGAAGCCCCCCTACAATTATGGGGTCCCTCCTCCGTATGTGAAACCGAATGCCAAAGCAAAAGATAGAAAACATGAAGTCAAGGATGGTTCTGGCAGCAAGCGCATCACTAAGGATCCTGTAGCGTATAACATAGCCTTTGTTGACAATATACTCAGAGGGGTAAGCGTAGGATCTGATGATGAGGATGAGAAGCCTGCTGCTACAAAAGTTAATGGTCATGATCATGAGAAGGATTGTGCTCATCGGGATGATGCATCTAGCAACCACATACCAAAACCAAGATCTTCTAGGAGGAGACACTCAAGATCTCGGTCCTCTCACAATGATGCTGGAAACAGTCAAGATACAGAAGTTGCAATCAGAAAAACAAGAAGCAGGAGAAAGGATGAGTCACGACGAGGTCTGCAACTGTTGTTTGATGACGAGCACTATAAGAAGGATGAAGAAGAGAGGAGAATGGACGAGCTTCTGATTCATTACAGTAAAAAGCCATCTAACTTGGACTCAGAATTGTCAAGAAGAAAGTCGAGAAGTCGTCACACACAGCATGCAGGCACTGGTGTTGGTGAATCCCCAAGACACGCAAGCAGAGATGATTCCGAAATGGTTCCAACAGCAGTAAGATCAGTTTCCCTTCCTCATGAACATAGCGGTCCATCAGAGGCACCCAAAGTATTTGCTCGTGCAGCTTCGTTTCAACCCGACAGGTCCAATCCAGCTAAGCATGTGCATCCCAAGCTACCGGACTACGAAGATTTGGCCGCCCAATTTGCAGCCCTAAGAGGAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

699

Amino Acids

79.1

Weight (kDa)

9.14

Isoelectric Point (pI)

52.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 170 1.3e-41 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1969
AccI GTMKAC 1 cut(s) 623
AccII CGCG 1 cut(s) 101
AciI CCGC 5 cut(s) 191, 330, 491, 1956, 2067
AclWI GGATC 5 cut(s) 80, 193, 1412, 1425, 1483
AcoI YGGCCR 3 cut(s) 19, 832, 2064
AcsI RAATTY 2 cut(s) 394, 840
AcuI CTGAAG 1 cut(s) 137
AfaI GTAC 1 cut(s) 1182
AfiI CCNNNNNNNGG 1 cut(s) 675
AgsI TTSAA 8 cut(s) 261, 380, 444, 481, 530, 736, 1306, 2003
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 2 cut(s) 803, 1008
AluBI AGCT 7 cut(s) 440, 721, 1046, 1771, 1994, 2024, 2044
AluI AGCT 7 cut(s) 440, 721, 1046, 1771, 1994, 2024, 2044
Alw21I GWGCWC 3 cut(s) 249, 1546, 1734
Alw26I GTCTC 3 cut(s) 960, 1149, 1594
AlwI GGATC 5 cut(s) 80, 193, 1412, 1425, 1483
AlwNI CAGNNNCTG 1 cut(s) 1862
AoxI GGCC 7 cut(s) 19, 148, 522, 587, 640, 832, 2064
ApeKI GCWGC 6 cut(s) 13, 326, 1398, 1502, 1991, 2078
ApoI RAATTY 2 cut(s) 394, 840
Asp700I GAANNNNTTC 1 cut(s) 1911
AspLEI GCGC 1 cut(s) 1407
AspS9I GGNCC 7 cut(s) 587, 641, 1199, 1327, 1617, 1958, 2013
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 2 cut(s) 133, 1883
AvaII GGWCC 5 cut(s) 1199, 1327, 1617, 1958, 2013
AxyI CCTNAGG 2 cut(s) 288, 365
BaeI ACNNNNGTAYC 2 cut(s) 1641, 1674
BalI TGGCCA 1 cut(s) 834
BamHI GGATCC 1 cut(s) 1417
BanI GGYRCC 1 cut(s) 1969
BarI GAAGNNNNNNTAC 2 cut(s) 1298, 1330
BauI CACGAG 3 cut(s) 1127, 1176, 1986
BbsI GAAGAC 1 cut(s) 71
Bbv12I GWGCWC 3 cut(s) 249, 1546, 1734
BbvI GCAGC 5 cut(s) 313, 1410, 1489, 2003, 2090
BccI CCATC 7 cut(s) 173, 532, 824, 986, 1382, 1804, 1969
BceAI ACGGC 1 cut(s) 6
BciT130I CCWGG 2 cut(s) 805, 1010
BclI TGATCA 2 cut(s) 745, 1525
BcnI CCSGG 1 cut(s) 851
BcoDI GTCTC 3 cut(s) 960, 1149, 1594
BfaI CTAG 4 cut(s) 762, 924, 1563, 1593
BfmI CTRYAG 2 cut(s) 636, 1422
BglII AGATCT 2 cut(s) 1586, 1610
BisI GCNGC 8 cut(s) 14, 327, 330, 1399, 1503, 1992, 2067, 2079
BlpI GCTNAGC 1 cut(s) 2025
BlsI GCNGC 8 cut(s) 15, 328, 331, 1400, 1504, 1993, 2068, 2080
Bme1390I CCNGG 3 cut(s) 805, 851, 1010
Bme18I GGWCC 5 cut(s) 1199, 1327, 1617, 1958, 2013
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 7 cut(s) 587, 641, 1199, 1327, 1617, 1958, 2013
BmiI GGNNCC 6 cut(s) 303, 1328, 1329, 1419, 1914, 1971
BmrFI CCNGG 3 cut(s) 805, 851, 1010
BmrI ACTGGG 2 cut(s) 636, 1182
BmsI GCATC 9 cut(s) 221, 685, 1054, 1284, 1416, 1546, 1568, 1621, 2044
BmuI ACTGGG 2 cut(s) 636, 1182
BpiI GAAGAC 1 cut(s) 71
BpmI CTGGAG 2 cut(s) 422, 479
Bpu1102I GCTNAGC 1 cut(s) 2025
BpuEI CTTGAG 3 cut(s) 233, 1086, 1591
BpuMI CCSGG 1 cut(s) 851
BsaJI CCNNGG 5 cut(s) 22, 519, 804, 835, 936
BsaWI WCCGGW 1 cut(s) 2047
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 675
Bse1I ACTGG 5 cut(s) 462, 631, 966, 1188, 1867
Bse21I CCTNAGG 2 cut(s) 288, 365
BseBI CCWGG 2 cut(s) 805, 1010
BseDI CCNNGG 5 cut(s) 22, 519, 804, 835, 936
BseGI GGATG 8 cut(s) 543, 1069, 1393, 1495, 1558, 1696, 1750, 2035
BseLI CCNNNNNNNGG 1 cut(s) 675
BseMII CTCAG 4 cut(s) 713, 973, 1471, 1824
BseNI ACTGG 5 cut(s) 462, 631, 966, 1188, 1867
BseRI GAGGAG 4 cut(s) 540, 1323, 1612, 1771
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 5 cut(s) 313, 1410, 1489, 2003, 2090
BsgI GTGCAG 1 cut(s) 2010
Bsh1236I CGCG 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 7 cut(s) 21, 150, 524, 589, 642, 834, 2066
BshNI GGYRCC 1 cut(s) 1969
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 3 cut(s) 249, 1546, 1734
BsiSI CCGG 2 cut(s) 850, 2048
BslFI GGGAC 2 cut(s) 527, 1313
BslI CCNNNNNNNGG 1 cut(s) 675
BsmAI GTCTC 3 cut(s) 960, 1149, 1594
BsmFI GGGAC 2 cut(s) 527, 1313
BsmI GAATGC 1 cut(s) 1357
BsnI GGCC 7 cut(s) 21, 150, 524, 589, 642, 834, 2066
Bsp1286I GDGCHC 3 cut(s) 249, 1546, 1734
Bsp1407I TGTACA 1 cut(s) 1180
Bsp1720I GCTNAGC 1 cut(s) 2025
Bsp19I CCATGG 1 cut(s) 835
BspACI CCGC 5 cut(s) 191, 330, 491, 1956, 2067
BspANI GGCC 7 cut(s) 21, 150, 524, 589, 642, 834, 2066
BspCNI CTCAG 4 cut(s) 712, 972, 1470, 1823
BspFNI CGCG 1 cut(s) 101
BspHI TCATGA 3 cut(s) 1522, 1528, 1945
BspLI GGNNCC 6 cut(s) 303, 1328, 1329, 1419, 1914, 1971
BspMAI CTGCAG 1 cut(s) 640
BspPI GGATC 5 cut(s) 80, 193, 1412, 1425, 1483
BspT107I GGYRCC 1 cut(s) 1969
BsrGI TGTACA 1 cut(s) 1180
BsrI ACTGG 5 cut(s) 462, 631, 966, 1188, 1867
BssECI CCNNGG 5 cut(s) 22, 519, 804, 835, 936
BssSI CACGAG 3 cut(s) 1127, 1176, 1986
BssT1I CCWWGG 3 cut(s) 519, 835, 936
Bst2BI CACGAG 3 cut(s) 1127, 1176, 1986
Bst2UI CCWGG 2 cut(s) 805, 1010
Bst4CI ACNGT 7 cut(s) 817, 979, 1094, 1220, 1643, 1716, 1787
Bst6I CTCTTC 3 cut(s) 86, 1284, 1746
BstAUI TGTACA 1 cut(s) 1180
BstC8I GCNNGC 6 cut(s) 640, 1403, 1500, 1854, 1858, 1891
BstDSI CCRYGG 2 cut(s) 22, 835
BstF5I GGATG 8 cut(s) 543, 1069, 1393, 1495, 1558, 1696, 1750, 2035
BstFNI CGCG 1 cut(s) 101
BstHHI GCGC 1 cut(s) 1407
BstMAI GTCTC 3 cut(s) 960, 1149, 1594
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 4 cut(s) 1404, 1991, 2041, 2075
BstNI CCWGG 2 cut(s) 805, 1010
BstNSI RCATGY 2 cut(s) 1856, 2033
BstSCI CCNGG 3 cut(s) 803, 849, 1008
BstSFI CTRYAG 2 cut(s) 636, 1422
BstUI CGCG 1 cut(s) 101
BstV1I GCAGC 5 cut(s) 313, 1410, 1489, 2003, 2090
BstV2I GAAGAC 1 cut(s) 71
BstX2I RGATCY 4 cut(s) 1417, 1475, 1586, 1610
BstYI RGATCY 4 cut(s) 1417, 1475, 1586, 1610
BstZI CGGCCG 1 cut(s) 19
Bsu36I CCTNAGG 2 cut(s) 288, 365
BsuRI GGCC 7 cut(s) 21, 150, 524, 589, 642, 834, 2066
BtgI CCRYGG 2 cut(s) 22, 835
BtgZI GCGATG 2 cut(s) 244, 297
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 8 cut(s) 543, 1069, 1393, 1495, 1558, 1696, 1750, 2035
BtsIMutI CAGTG 6 cut(s) 455, 805, 967, 973, 1195, 1860
Cac8I GCNNGC 6 cut(s) 640, 1403, 1500, 1854, 1858, 1891
CaiI CAGNNNCTG 1 cut(s) 1862
CciI TCATGA 3 cut(s) 1522, 1528, 1945
CfoI GCGC 1 cut(s) 1407
Cfr13I GGNCC 7 cut(s) 587, 641, 1199, 1327, 1617, 1958, 2013
CseI GACGC 2 cut(s) 112, 1274
Csp6I GTAC 1 cut(s) 1181
CspCI CAANNNNNGTGG 2 cut(s) 1231, 1266
CviQI GTAC 1 cut(s) 1181
EaeI YGGCCR 3 cut(s) 19, 832, 2064
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 3 cut(s) 86, 1284, 1746
EarI CTCTTC 3 cut(s) 86, 1284, 1746
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 3 cut(s) 519, 835, 936
Eco147I AGGCCT 1 cut(s) 524
Eco32I GATATC 2 cut(s) 127, 157
Eco47I GGWCC 5 cut(s) 1199, 1327, 1617, 1958, 2013
Eco52I CGGCCG 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 137
Eco81I CCTNAGG 2 cut(s) 288, 365
EcoO109I RGGNCCY 1 cut(s) 1327
EcoRII CCWGG 2 cut(s) 803, 1008
EcoRV GATATC 2 cut(s) 127, 157
EcoT14I CCWWGG 3 cut(s) 519, 835, 936
EcoT22I ATGCAT 1 cut(s) 1561
ErhI CCWWGG 3 cut(s) 519, 835, 936
FaqI GGGAC 2 cut(s) 527, 1313
FbaI TGATCA 2 cut(s) 745, 1525
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 8 cut(s) 14, 327, 330, 1399, 1503, 1992, 2067, 2079
FokI GGATG 8 cut(s) 550, 1076, 1400, 1502, 1565, 1703, 1757, 2022
Fsp4HI GCNGC 8 cut(s) 14, 327, 330, 1399, 1503, 1992, 2067, 2079
FspBI CTAG 4 cut(s) 762, 924, 1563, 1593
GlaI GCGC 1 cut(s) 1406
GluI GCNGC 8 cut(s) 14, 327, 330, 1399, 1503, 1992, 2067, 2079
GsuI CTGGAG 2 cut(s) 422, 479
HaeIII GGCC 7 cut(s) 21, 150, 524, 589, 642, 834, 2066
HapII CCGG 2 cut(s) 850, 2048
HgaI GACGC 2 cut(s) 112, 1274
HhaI GCGC 1 cut(s) 1407
Hin6I GCGC 1 cut(s) 1405
HinP1I GCGC 1 cut(s) 1405
HincII GTYRAC 1 cut(s) 1447
HindII GTYRAC 1 cut(s) 1447
HindIII AAGCTT 1 cut(s) 1044
HpaII CCGG 2 cut(s) 850, 2048
HphI GGTGA 2 cut(s) 133, 1883
Hpy166II GTNNAC 4 cut(s) 561, 624, 1181, 1447
Hpy8I GTNNAC 4 cut(s) 561, 624, 1181, 1447
Hpy99I CGWCG 3 cut(s) 80, 106, 1704
HpyAV CCTTC 6 cut(s) 115, 165, 600, 1528, 1735, 1949
HpyCH4III ACNGT 7 cut(s) 817, 979, 1094, 1220, 1643, 1716, 1787
HpyCH4IV ACGT 2 cut(s) 78, 912
HpyF10VI GCNNNNNNNGC 4 cut(s) 1404, 1991, 2041, 2075
HpySE526I ACGT 2 cut(s) 78, 912
HspAI GCGC 1 cut(s) 1405
KflI GGGWCCC 1 cut(s) 1327
Ksp22I TGATCA 2 cut(s) 745, 1525
LmnI GCTCC 2 cut(s) 301, 1072
Lsp1109I GCAGC 5 cut(s) 313, 1410, 1489, 2003, 2090
LweI GCATC 9 cut(s) 221, 685, 1054, 1284, 1416, 1546, 1568, 1621, 2044
MaeI CTAG 4 cut(s) 762, 924, 1563, 1593
MaeII ACGT 2 cut(s) 78, 912
MaeIII GTNAC 5 cut(s) 359, 602, 1124, 1695, 1841
MflI RGATCY 4 cut(s) 1417, 1475, 1586, 1610
MhlI GDGCHC 3 cut(s) 249, 1546, 1734
MlsI TGGCCA 1 cut(s) 834
MluNI TGGCCA 1 cut(s) 834
MlyI GAGTC 5 cut(s) 61, 177, 509, 1703, 1802
MmeI TCCRAC 1 cut(s) 1940
Mox20I TGGCCA 1 cut(s) 834
Mph1103I ATGCAT 1 cut(s) 1561
MroXI GAANNNNTTC 1 cut(s) 1911
MscI TGGCCA 1 cut(s) 834
MseI TTAA 4 cut(s) 54, 690, 1281, 1515
MslI CAYNNNNRTG 6 cut(s) 600, 879, 1038, 1527, 1551, 1629
Msp20I TGGCCA 1 cut(s) 834
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 2 cut(s) 850, 2048
MspR9I CCNGG 3 cut(s) 805, 851, 1010
Mva1269I GAATGC 1 cut(s) 1357
MvaI CCWGG 2 cut(s) 805, 1010
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 4 cut(s) 1404, 1991, 2041, 2075
NciI CCSGG 1 cut(s) 851
NcoI CCATGG 1 cut(s) 835
NlaIV GGNNCC 6 cut(s) 303, 1328, 1329, 1419, 1914, 1971
NmuCI GTSAC 5 cut(s) 359, 602, 1124, 1695, 1841
NsiI ATGCAT 1 cut(s) 1561
NspI RCATGY 2 cut(s) 1856, 2033
PaeI GCATGC 1 cut(s) 1856
PagI TCATGA 3 cut(s) 1522, 1528, 1945
PceI AGGCCT 1 cut(s) 524
PctI GAATGC 1 cut(s) 1357
PdmI GAANNNNTTC 1 cut(s) 1911
PfeI GAWTC 6 cut(s) 253, 415, 469, 1777, 1874, 1901
PflFI GACNNNGTC 1 cut(s) 1704
PfoI TCCNGGA 2 cut(s) 849, 1008
PkrI GCNGC 8 cut(s) 15, 328, 331, 1400, 1504, 1993, 2068, 2080
PleI GAGTC 5 cut(s) 61, 177, 509, 1702, 1802
PpsI GAGTC 5 cut(s) 61, 177, 509, 1702, 1802
PpuMI RGGWCCY 1 cut(s) 1327
Psp5II RGGWCCY 1 cut(s) 1327
Psp6I CCWGG 2 cut(s) 803, 1008
PspGI CCWGG 2 cut(s) 803, 1008
PspN4I GGNNCC 6 cut(s) 303, 1328, 1329, 1419, 1914, 1971
PspPI GGNCC 7 cut(s) 587, 641, 1199, 1327, 1617, 1958, 2013
PspPPI RGGWCCY 1 cut(s) 1327
PstI CTGCAG 1 cut(s) 640
PstNI CAGNNNCTG 1 cut(s) 1862
PsuI RGATCY 4 cut(s) 1417, 1475, 1586, 1610
PsyI GACNNNGTC 1 cut(s) 1704
RsaI GTAC 1 cut(s) 1182
RsaNI GTAC 1 cut(s) 1181
RseI CAYNNNNRTG 6 cut(s) 600, 879, 1038, 1527, 1551, 1629
SaqAI TTAA 4 cut(s) 54, 690, 1281, 1515
SatI GCNGC 8 cut(s) 14, 327, 330, 1399, 1503, 1992, 2067, 2079
Sau96I GGNCC 7 cut(s) 587, 641, 1199, 1327, 1617, 1958, 2013
SchI GAGTC 5 cut(s) 61, 177, 509, 1703, 1802
ScrFI CCNGG 3 cut(s) 805, 851, 1010
SduI GDGCHC 3 cut(s) 249, 1546, 1734
SfaNI GCATC 9 cut(s) 221, 685, 1054, 1284, 1416, 1546, 1568, 1621, 2044
SfcI CTRYAG 2 cut(s) 636, 1422
SinI GGWCC 5 cut(s) 1199, 1327, 1617, 1958, 2013
SmiMI CAYNNNNRTG 6 cut(s) 600, 879, 1038, 1527, 1551, 1629
SmlI CTYRAG 3 cut(s) 248, 1101, 1606
SmoI CTYRAG 3 cut(s) 248, 1101, 1606
SphI GCATGC 1 cut(s) 1856
SseBI AGGCCT 1 cut(s) 524
SsiI CCGC 5 cut(s) 191, 330, 491, 1956, 2067
SspMI CTAG 4 cut(s) 762, 924, 1563, 1593
StuI AGGCCT 1 cut(s) 524
StyD4I CCNGG 3 cut(s) 803, 849, 1008
StyI CCWWGG 3 cut(s) 519, 835, 936
TaaI ACNGT 7 cut(s) 817, 979, 1094, 1220, 1643, 1716, 1787
TaiI ACGT 2 cut(s) 81, 915
TaqI TCGA 2 cut(s) 75, 1832
TaqII GACCGA 1 cut(s) 1605
TatI WGTACW 1 cut(s) 1180
TauI GCSGC 2 cut(s) 332, 2069
TfiI GAWTC 6 cut(s) 253, 415, 469, 1777, 1874, 1901
Tru1I TTAA 4 cut(s) 54, 690, 1281, 1515
Tru9I TTAA 4 cut(s) 54, 690, 1281, 1515
TscAI CASTG 6 cut(s) 462, 805, 967, 973, 1195, 1867
TseFI GTSAC 5 cut(s) 359, 602, 1124, 1695, 1841
TseI GCWGC 6 cut(s) 13, 326, 1398, 1502, 1991, 2078
Tsp45I GTSAC 5 cut(s) 359, 602, 1124, 1695, 1841
TspDTI ATGAA 9 cut(s) 897, 905, 1056, 1086, 1179, 1392, 1761, 1769, 1962
TspGWI ACGGA 2 cut(s) 100, 1326
TspRI CASTG 6 cut(s) 462, 805, 967, 973, 1195, 1867
Tth111I GACNNNGTC 1 cut(s) 1704
VpaK11BI GGWCC 5 cut(s) 1199, 1327, 1617, 1958, 2013
XapI RAATTY 2 cut(s) 394, 840
XceI RCATGY 2 cut(s) 1856, 2033
XmiI GTMKAC 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 1911
XspI CTAG 4 cut(s) 762, 924, 1563, 1593
Zsp2I ATGCAT 1 cut(s) 1561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.