AT4G37550

formamidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003336
Physical Location & Seq
Forward (+)
17643110 .. 17645830
2721 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G37550.3

Sequence Viewer

Length: 1359 bp
ATGGCTCCACTGAGTCCAAGAGTAGTGGTGAAGGTGGATTTGAAGAAGAAGCCATGGCAGCAGGACCAGCCATTGCACAATAGATGGCATCCGGAGATACCACCAGTAGCAGAGGTCAAGGCTGGTGAGTTTTTCCGGGTAGAAATGATTGATGCAATGGGAGGTGTTATCAAAGACAATGACTCTGCAAGCGATATCAAGAACCTAGTCCTAACTACTACTCATCACCTAAGTGGGCCGATTAGGGTTGTAGATGAGGAAGGTGTTGCAGCTAAAGCAGGTGATCTTCTTGCTGTTGAGATATGCAACTTGGGTCCTCTTCCAGGAGATGAATGGGGATTCACTGGTTCGTTTGATAGAGAGAATGGTGGCGGTTTCTTGACTGACCATTTCCCTTGTGCGACCAAAGCTATTTGGTACTTTGAAGGGATCTATGCCTACTCTCCTCAGATTCCTGGAGTACGATTTCCGGGTTTGACACACCCAGGAGTCATTGGCACTGCACCATCGAATGAACTCTTGAGAATATGGAATGATAGGGAAAGACAATTAGAAGAAAGTGGTGTTGAGTCCCTAACACTATGTGAGGTAGTGCATCAGCGGCCATTAGCATGCCTACCAACAACTAAAGGATGTCTCCTCGGGAATATTGAAGAAGGAACACCAGAATGGGAAAGGATTGCAAACGAGGCTGCGAGGACAATACCAGGAAGAGAAAACGGAGGAAACTGTGACATTAAAAACCTAAGCAGAGGCTCTAAAATATATCTTCCAGTATTTGTTGAAGGGGCTAATCTAAGCACAGGAGACATGCATTTCTCACAAGGTGATGGTGAGATCTCGTTCTGCGGAGCCATCGAGATGAGTGGATTTCTAGAACTCAAGTGTGAAATCATAAGAAATGGGATGCAAGAGTACCTTACGCCAATGGGACCTACCCCTCTCCATGTAAACCCAATCTTTGAGATAGGACCAGTGGAACCGAGGTTCTCAGAGTGGCTTGTCTTCGAAGGTATCAGTGTCGATGAGAGCGGAAAACAGCATTACCTAGATGCAACAGTTGCTTACAAACGGGCAGTTCTCAACGCCATAGACTATCTTTTCAAATTTGGTTACTCAAAAGAACAGGTGTACCTCTTACTCTCATGTTGTCCATGTGAAGGAAGACTATCTGGGATAGTTGATTCTCCGAATGCCGTTGCTACACTAGCAATACCTACAGCCATCTTTGATCAGGATATTAGGCCAAAGAATCGGAAGGTTCCAGTAGGACCTCGGGTTGTGAGGAAACCAGATGTACTGAAGAGCACATATGATGGAAAGCTTCCAATTACAAAGAATCCAAGTTCTTCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

452

Amino Acids

49.67

Weight (kDa)

5.36

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 11 - 392 3.5e-165 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 269
AccBSI CCGCTC 1 cut(s) 1032
AccIII TCCGGA 1 cut(s) 91
AciI CCGC 4 cut(s) 372, 601, 849, 1032
AclWI GGATC 1 cut(s) 437
AcoI YGGCCR 1 cut(s) 602
AcsI RAATTY 1 cut(s) 1106
AcuI CTGAAG 1 cut(s) 1322
AdeI CACNNNGTG 2 cut(s) 584, 827
AfaI GTAC 5 cut(s) 419, 462, 917, 1133, 1299
AfiI CCNNNNNNNGG 2 cut(s) 323, 1160
AgsI TTSAA 5 cut(s) 43, 425, 653, 785, 1105
AjnI CCWGG 4 cut(s) 322, 454, 484, 706
AleI CACNNNNGTG 1 cut(s) 231
AloI GAACNNNNNNTCC 2 cut(s) 971, 1003
AluBI AGCT 3 cut(s) 272, 410, 1324
AluI AGCT 3 cut(s) 272, 410, 1324
Alw21I GWGCWC 1 cut(s) 1310
Alw26I GTCTC 2 cut(s) 641, 801
AlwI GGATC 1 cut(s) 437
Ama87I CYCGRG 2 cut(s) 641, 1275
Aor13HI TCCGGA 1 cut(s) 91
AoxI GGCC 3 cut(s) 236, 602, 1244
ApeKI GCWGC 3 cut(s) 58, 269, 692
ApoI RAATTY 1 cut(s) 1106
AspS9I GGNCC 6 cut(s) 64, 236, 314, 932, 971, 1271
AsuC2I CCSGG 2 cut(s) 137, 471
AsuHPI GGTGA 6 cut(s) 40, 137, 218, 293, 839, 845
AsuII TTCGAA 1 cut(s) 1008
AvaI CYCGRG 2 cut(s) 641, 1275
AvaII GGWCC 5 cut(s) 64, 314, 932, 971, 1271
BaeI ACNNNNGTAYC 2 cut(s) 1115, 1148
BbsI GAAGAC 2 cut(s) 997, 1171
Bbv12I GWGCWC 1 cut(s) 1310
BbvI GCAGC 3 cut(s) 70, 281, 679
BccI CCATC 6 cut(s) 78, 514, 824, 863, 1232, 1310
BceAI ACGGC 1 cut(s) 1181
BciT130I CCWGG 4 cut(s) 324, 456, 486, 708
BclI TGATCA 1 cut(s) 1231
BcnI CCSGG 2 cut(s) 137, 471
BcoDI GTCTC 2 cut(s) 641, 801
BfaI CTAG 4 cut(s) 206, 875, 1049, 1208
BfmI CTRYAG 1 cut(s) 1218
BfuAI ACCTGC 1 cut(s) 269
BglII AGATCT 1 cut(s) 837
BisI GCNGC 4 cut(s) 59, 270, 602, 693
BlsI GCNGC 4 cut(s) 60, 271, 603, 694
Bme1390I CCNGG 6 cut(s) 137, 324, 456, 471, 486, 708
Bme18I GGWCC 5 cut(s) 64, 314, 932, 971, 1271
BmeT110I CYCGRG 2 cut(s) 641, 1275
BmgT120I GGNCC 6 cut(s) 64, 236, 314, 932, 971, 1271
BmiI GGNNCC 6 cut(s) 6, 315, 853, 933, 981, 1263
BmrFI CCNGG 6 cut(s) 137, 324, 456, 471, 486, 708
BmsI GCATC 5 cut(s) 97, 142, 604, 897, 1042
BpiI GAAGAC 2 cut(s) 997, 1171
BpmI CTGGAG 1 cut(s) 477
Bpu10I CCTNAGC 1 cut(s) 746
Bpu14I TTCGAA 1 cut(s) 1008
BpuEI CTTGAG 2 cut(s) 541, 866
BpuMI CCSGG 2 cut(s) 137, 471
BsaJI CCNNGG 5 cut(s) 53, 484, 640, 983, 1274
BsaWI WCCGGW 1 cut(s) 91
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 1160
Bse1I ACTGG 5 cut(s) 104, 349, 773, 974, 1265
Bse3DI GCAATG 2 cut(s) 71, 162
BseAI TCCGGA 1 cut(s) 91
BseBI CCWGG 4 cut(s) 324, 456, 486, 708
BseDI CCNNGG 5 cut(s) 53, 484, 640, 983, 1274
BseGI GGATG 3 cut(s) 88, 638, 912
BseLI CCNNNNNNNGG 2 cut(s) 323, 1160
BseMI GCAATG 2 cut(s) 71, 162
BseMII CTCAG 2 cut(s) 461, 1005
BseNI ACTGG 5 cut(s) 104, 349, 773, 974, 1265
BseRI GAGGAG 2 cut(s) 435, 629
BseXI GCAGC 3 cut(s) 70, 281, 679
BsgI GTGCAG 1 cut(s) 486
BshFI GGCC 3 cut(s) 238, 604, 1246
BsiHKAI GWGCWC 1 cut(s) 1310
BsiHKCI CYCGRG 2 cut(s) 641, 1275
BsiSI CCGG 3 cut(s) 92, 136, 470
BslFI GGGAC 2 cut(s) 556, 945
BslI CCNNNNNNNGG 2 cut(s) 323, 1160
BsmAI GTCTC 2 cut(s) 641, 801
BsmFI GGGAC 2 cut(s) 556, 945
BsmI GAATGC 1 cut(s) 1198
BsnI GGCC 3 cut(s) 238, 604, 1246
BsoBI CYCGRG 2 cut(s) 641, 1275
Bsp119I TTCGAA 1 cut(s) 1008
Bsp1286I GDGCHC 1 cut(s) 1310
Bsp13I TCCGGA 1 cut(s) 91
Bsp143I GATC 4 cut(s) 283, 429, 837, 1231
Bsp19I CCATGG 1 cut(s) 53
BspACI CCGC 4 cut(s) 372, 601, 849, 1032
BspANI GGCC 3 cut(s) 238, 604, 1246
BspCNI CTCAG 2 cut(s) 460, 1004
BspEI TCCGGA 1 cut(s) 91
BspLI GGNNCC 6 cut(s) 6, 315, 853, 933, 981, 1263
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 1 cut(s) 437
BspQI GCTCTTC 1 cut(s) 1298
BspT104I TTCGAA 1 cut(s) 1008
BsrBI CCGCTC 1 cut(s) 1032
BsrDI GCAATG 2 cut(s) 71, 162
BsrI ACTGG 5 cut(s) 104, 349, 773, 974, 1265
BssECI CCNNGG 5 cut(s) 53, 484, 640, 983, 1274
BssMI GATC 4 cut(s) 283, 429, 837, 1231
BssT1I CCWWGG 1 cut(s) 53
Bst2UI CCWGG 4 cut(s) 324, 456, 486, 708
Bst4CI ACNGT 2 cut(s) 731, 1060
Bst6I CTCTTC 3 cut(s) 324, 706, 1298
BstAPI GCANNNNNTGC 1 cut(s) 1061
BstBI TTCGAA 1 cut(s) 1008
BstC8I GCNNGC 2 cut(s) 190, 613
BstDEI CTNAG 7 cut(s) 11, 230, 447, 746, 797, 991, 1356
BstDSI CCRYGG 1 cut(s) 53
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 3 cut(s) 88, 638, 912
BstKTI GATC 4 cut(s) 286, 432, 840, 1234
BstMAI GTCTC 2 cut(s) 641, 801
BstMBI GATC 4 cut(s) 283, 429, 837, 1231
BstMWI GCNNNNNNNGC 8 cut(s) 58, 67, 275, 407, 601, 689, 1061, 1208
BstNI CCWGG 4 cut(s) 324, 456, 486, 708
BstNSI RCATGY 2 cut(s) 615, 814
BstSCI CCNGG 6 cut(s) 135, 322, 454, 469, 484, 706
BstSFI CTRYAG 1 cut(s) 1218
BstV1I GCAGC 3 cut(s) 70, 281, 679
BstV2I GAAGAC 2 cut(s) 997, 1171
BstX2I RGATCY 2 cut(s) 429, 837
BstYI RGATCY 2 cut(s) 429, 837
BsuRI GGCC 3 cut(s) 238, 604, 1246
BtgI CCRYGG 1 cut(s) 53
BtsCI GGATG 3 cut(s) 88, 638, 912
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 5 cut(s) 8, 342, 498, 981, 1024
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 2 cut(s) 190, 613
Cfr13I GGNCC 6 cut(s) 64, 236, 314, 932, 971, 1271
Csp6I GTAC 5 cut(s) 418, 461, 916, 1132, 1298
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 6 cut(s) 54, 612, 811, 947, 1146, 1155
CviQI GTAC 5 cut(s) 418, 461, 916, 1132, 1298
DdeI CTNAG 7 cut(s) 11, 230, 447, 746, 797, 991, 1356
DpnI GATC 4 cut(s) 285, 431, 839, 1233
DpnII GATC 4 cut(s) 283, 429, 837, 1231
DraIII CACNNNGTG 2 cut(s) 584, 827
EaeI YGGCCR 1 cut(s) 602
Eam1104I CTCTTC 3 cut(s) 324, 706, 1298
EarI CTCTTC 3 cut(s) 324, 706, 1298
Eco130I CCWWGG 1 cut(s) 53
Eco32I GATATC 1 cut(s) 196
Eco47I GGWCC 5 cut(s) 64, 314, 932, 971, 1271
Eco57I CTGAAG 1 cut(s) 1322
Eco88I CYCGRG 2 cut(s) 641, 1275
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoO109I RGGNCCY 3 cut(s) 314, 932, 1271
EcoRII CCWGG 4 cut(s) 322, 454, 484, 706
EcoRV GATATC 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 53
EcoT22I ATGCAT 1 cut(s) 816
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 6 cut(s) 57, 615, 814, 950, 1149, 1158
FalI AAGNNNNNCTT 2 cut(s) 903, 935
FaqI GGGAC 2 cut(s) 556, 945
FatI CATG 6 cut(s) 53, 611, 810, 946, 1145, 1154
FauNDI CATATG 1 cut(s) 1312
FbaI TGATCA 1 cut(s) 1231
Fnu4HI GCNGC 4 cut(s) 59, 270, 602, 693
FokI GGATG 3 cut(s) 75, 645, 919
Fsp4HI GCNGC 4 cut(s) 59, 270, 602, 693
FspBI CTAG 4 cut(s) 206, 875, 1049, 1208
GluI GCNGC 4 cut(s) 59, 270, 602, 693
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 3 cut(s) 238, 604, 1246
HapII CCGG 3 cut(s) 92, 136, 470
Hin1II CATG 6 cut(s) 57, 615, 814, 950, 1149, 1158
HindIII AAGCTT 1 cut(s) 1322
HinfI GANTC 9 cut(s) 13, 182, 339, 451, 489, 569, 1184, 1252, 1339
HpaII CCGG 3 cut(s) 92, 136, 470
HphI GGTGA 6 cut(s) 40, 137, 218, 293, 839, 845
Hpy166II GTNNAC 2 cut(s) 952, 1132
Hpy188I TCNGA 4 cut(s) 450, 994, 1191, 1257
Hpy188III TCNNGA 8 cut(s) 92, 199, 379, 520, 643, 859, 875, 1235
Hpy8I GTNNAC 2 cut(s) 952, 1132
HpyAV CCTTC 8 cut(s) 25, 254, 419, 650, 779, 1004, 1154, 1252
HpyCH4III ACNGT 2 cut(s) 731, 1060
HpyF10VI GCNNNNNNNGC 8 cut(s) 58, 67, 275, 407, 601, 689, 1061, 1208
HpyF3I CTNAG 7 cut(s) 11, 230, 447, 746, 797, 991, 1356
Hsp92II CATG 6 cut(s) 57, 615, 814, 950, 1149, 1158
Kpn2I TCCGGA 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 1231
Kzo9I GATC 4 cut(s) 283, 429, 837, 1231
LguI GCTCTTC 1 cut(s) 1298
LmnI GCTCC 2 cut(s) 10, 851
Lsp1109I GCAGC 3 cut(s) 70, 281, 679
LweI GCATC 5 cut(s) 97, 142, 604, 897, 1042
MaeI CTAG 4 cut(s) 206, 875, 1049, 1208
MaeIII GTNAC 2 cut(s) 731, 1112
MalI GATC 4 cut(s) 285, 431, 839, 1233
MbiI CCGCTC 1 cut(s) 1032
MboI GATC 4 cut(s) 283, 429, 837, 1231
MflI RGATCY 2 cut(s) 429, 837
MhlI GDGCHC 1 cut(s) 1310
MluCI AATT 3 cut(s) 548, 1106, 1329
MlyI GAGTC 4 cut(s) 22, 176, 498, 578
Mph1103I ATGCAT 1 cut(s) 816
MroI TCCGGA 1 cut(s) 91
MseI TTAA 1 cut(s) 738
MslI CAYNNNNRTG 4 cut(s) 231, 610, 667, 860
MspA1I CMGCKG 1 cut(s) 601
MspI CCGG 3 cut(s) 92, 136, 470
MspR9I CCNGG 6 cut(s) 137, 324, 456, 471, 486, 708
Mva1269I GAATGC 1 cut(s) 1198
MvaI CCWGG 4 cut(s) 324, 456, 486, 708
MwoI GCNNNNNNNGC 8 cut(s) 58, 67, 275, 407, 601, 689, 1061, 1208
NciI CCSGG 2 cut(s) 137, 471
NcoI CCATGG 1 cut(s) 53
NdeI CATATG 1 cut(s) 1312
NdeII GATC 4 cut(s) 283, 429, 837, 1231
NlaIII CATG 6 cut(s) 57, 615, 814, 950, 1149, 1158
NlaIV GGNNCC 6 cut(s) 6, 315, 853, 933, 981, 1263
NmuCI GTSAC 1 cut(s) 731
NsiI ATGCAT 1 cut(s) 816
NspI RCATGY 2 cut(s) 615, 814
NspV TTCGAA 1 cut(s) 1008
OliI CACNNNNGTG 1 cut(s) 231
PaeI GCATGC 1 cut(s) 615
PaqCI CACCTGC 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 1298
PctI GAATGC 1 cut(s) 1198
PfeI GAWTC 5 cut(s) 339, 451, 1184, 1252, 1339
PfoI TCCNGGA 2 cut(s) 322, 454
PkrI GCNGC 4 cut(s) 60, 271, 603, 694
PleI GAGTC 4 cut(s) 21, 176, 497, 577
PpsI GAGTC 4 cut(s) 21, 176, 497, 577
PpuMI RGGWCCY 3 cut(s) 314, 932, 1271
Psp5II RGGWCCY 3 cut(s) 314, 932, 1271
Psp6I CCWGG 4 cut(s) 322, 454, 484, 706
PspGI CCWGG 4 cut(s) 322, 454, 484, 706
PspN4I GGNNCC 6 cut(s) 6, 315, 853, 933, 981, 1263
PspPI GGNCC 6 cut(s) 64, 236, 314, 932, 971, 1271
PspPPI RGGWCCY 3 cut(s) 314, 932, 1271
PsuI RGATCY 2 cut(s) 429, 837
RsaI GTAC 5 cut(s) 419, 462, 917, 1133, 1299
RsaNI GTAC 5 cut(s) 418, 461, 916, 1132, 1298
RseI CAYNNNNRTG 4 cut(s) 231, 610, 667, 860
SapI GCTCTTC 1 cut(s) 1298
SaqAI TTAA 1 cut(s) 738
SatI GCNGC 4 cut(s) 59, 270, 602, 693
Sau3AI GATC 4 cut(s) 283, 429, 837, 1231
Sau96I GGNCC 6 cut(s) 64, 236, 314, 932, 971, 1271
SchI GAGTC 4 cut(s) 22, 176, 498, 578
ScrFI CCNGG 6 cut(s) 137, 324, 456, 471, 486, 708
SduI GDGCHC 1 cut(s) 1310
SfaNI GCATC 5 cut(s) 97, 142, 604, 897, 1042
SfcI CTRYAG 1 cut(s) 1218
SfuI TTCGAA 1 cut(s) 1008
SinI GGWCC 5 cut(s) 64, 314, 932, 971, 1271
SmiMI CAYNNNNRTG 4 cut(s) 231, 610, 667, 860
SmlI CTYRAG 2 cut(s) 520, 881
SmoI CTYRAG 2 cut(s) 520, 881
SphI GCATGC 1 cut(s) 615
Sse9I AATT 3 cut(s) 548, 1106, 1329
SsiI CCGC 4 cut(s) 372, 601, 849, 1032
SspI AATATT 1 cut(s) 649
SspMI CTAG 4 cut(s) 206, 875, 1049, 1208
StyD4I CCNGG 6 cut(s) 135, 322, 454, 469, 484, 706
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 2 cut(s) 731, 1060
TaqI TCGA 4 cut(s) 509, 858, 1008, 1023
TasI AATT 3 cut(s) 548, 1106, 1329
TatI WGTACW 1 cut(s) 1297
TauI GCSGC 1 cut(s) 604
TfiI GAWTC 5 cut(s) 339, 451, 1184, 1252, 1339
Tru1I TTAA 1 cut(s) 738
Tru9I TTAA 1 cut(s) 738
TscAI CASTG 5 cut(s) 15, 349, 505, 981, 1024
TseFI GTSAC 1 cut(s) 731
TseI GCWGC 3 cut(s) 58, 269, 692
Tsp45I GTSAC 1 cut(s) 731
TspDTI ATGAA 2 cut(s) 345, 528
TspGWI ACGGA 1 cut(s) 735
TspRI CASTG 5 cut(s) 15, 349, 505, 981, 1024
VpaK11BI GGWCC 5 cut(s) 64, 314, 932, 971, 1271
XagI CCTNNNNNAGG 1 cut(s) 321
XapI RAATTY 1 cut(s) 1106
XbaI TCTAGA 1 cut(s) 874
XceI RCATGY 2 cut(s) 615, 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 330
XspI CTAG 4 cut(s) 206, 875, 1049, 1208
Zsp2I ATGCAT 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.