RchiOBHm_Chr2g0107581

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003336
Physical Location & Seq
Forward (+)
18928335 .. 18931713
3379 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48160

Sequence Viewer

Length: 1458 bp
ATGGCTGTCAAACTTCAATCCTATGATGAAAGCAGCATATCATCACTTCAACCACAACAAGGAATCTTCAATCCATCATCTTACCATTGCTCATCTTCAACAAGACCAAAAATGGCTTCTTCTTATGGAGCAAGACTGGTTGTGCCGATAGATGTGAAAAAGAAGCCACGGGAGCAGAAGCTTCCACTTCACAACCGGTGGCACCCGGATATACCACCTGTGGCAGAAGCTACGGTGGGAGAGGTTTTTCGAGTTGAAATGGTTGATTTCAGCGGGGAGGTATCACAAAGGAATACACTGCCGAGGACATCAAGAATGCCAATCCTTCAATTCGGACCGATTAGGATTTCAGACAAGGATGGAATTCCAGCACAGCCAGGTGATCTTCTGGTAGTTGAGATATGCAACTTGGGTCCTCTTCCAGGAGATGAATGGGGTTTCACAGCAACATTCGACAGAGAAAATGGAGGGGGATTTCTGACTGACCATTTTCCTTGTGCAACCAAGGCTATTTGGTATTTTGAAGGGATATATGCCTACTCTCCTCAAATACCAGGAGTGAGATTCCCAGGGTTAACACACCCTGGAATAGTTGGAACAGCACCATCAACGGAACTCTTGAACATATGGAATAAGAGGGAGAGAGAACTTGAGGAAAATGGAGTCAAGTCTCTGAAACTATGTGAGGTTGTGCACCAACGGCCCTTGGCTAGCCTACCATCAACAAAAGGTTGCGTCCTTGGAGGGATCAAAGAGGGCACTCCCGAATGGGAAAAGATAGCAGTGGAGGCCGCAAGAACAATTCCAGGAAGAGAAAATGGTGGCAATTGTGACATCAAGAATCTTAGTAGAGGTTCAAAGGTATACCTTCCGGTATTCATTGAAGGAGCAAATCTTAGCACTGGTGACATGCACTTTTCACAGGGAGATGGTGAAATCGCATTCTGTGGTGCGATTGAGATGAGTGGTTTCCTGGAGCTCAAGTGTGAAATTATAAGGGATGGAATGAAAGAATACCTAACACCGATGGGGCCAACCCCTCTTCATGTGAATCCAATCTTTGAAATAGGTCCCGTCGAGCCAAGATTCTCGGAATGGTTGGTATTTGAGGGCATTAGTGTTGATGAGAGTGGGAAGCAGCACTACCTAGATGCAACTGTTGCTTACAAGCGTGCAGTACTGAATGCCATCGACTACCTCTCAAAATTTGGATACTCAAAAGAACAGAGCTACCTTCTGCTGTCATGCTGTCCATGCGAGGGAAGAATTTCTGGAATAGTCGACGCTCCCAATGCTGTTGCAACCCTAGCAATCCCAACAGCTATCTTTGACCAGGATATTCGACCAAAAACGAACAAGGTCCCGGTTGGTCCCCGGATAGTGAGGACACCAGATGTCCTAAAATGTACTTACGATGGAAATTTGCCAACGACAAGGAACCCTAGCTCAGCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

485

Amino Acids

53.21

Weight (kDa)

5.94

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 50 - 445 2.7e-153 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 995
AccB1I GGYRCC 1 cut(s) 201
AccI GTMKAC 2 cut(s) 864, 1281
AciI CCGC 2 cut(s) 273, 792
AclWI GGATC 1 cut(s) 755
AcsI RAATTY 4 cut(s) 363, 1205, 1266, 1420
AfaI GTAC 2 cut(s) 1179, 1408
AfiI CCNNNNNNNGG 3 cut(s) 59, 422, 1259
AgeI ACCGGT 1 cut(s) 195
AjnI CCWGG 8 cut(s) 376, 421, 553, 568, 583, 805, 972, 1332
AjuI GAANNNNNNNTTGG 2 cut(s) 100, 132
AluBI AGCT 6 cut(s) 181, 230, 979, 1230, 1322, 1446
AluI AGCT 6 cut(s) 181, 230, 979, 1230, 1322, 1446
Alw21I GWGCWC 2 cut(s) 696, 981
Alw26I GTCTC 1 cut(s) 675
Alw44I GTGCAC 1 cut(s) 692
AlwI GGATC 1 cut(s) 755
AoxI GGCC 3 cut(s) 701, 789, 1031
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 2 cut(s) 33, 1138
ApoI RAATTY 4 cut(s) 363, 1205, 1266, 1420
AsiGI ACCGGT 1 cut(s) 195
Asp700I GAANNNNTTC 1 cut(s) 1267
AspS9I GGNCC 7 cut(s) 335, 413, 702, 1031, 1070, 1360, 1370
AsuC2I CCSGG 3 cut(s) 206, 1364, 1375
AsuHPI GGTGA 3 cut(s) 392, 917, 944
AsuNHI GCTAGC 1 cut(s) 710
AvaII GGWCC 5 cut(s) 335, 413, 1070, 1360, 1370
BaeGI GKGCMC 2 cut(s) 696, 761
BanI GGYRCC 1 cut(s) 201
BanII GRGCYC 1 cut(s) 981
BarI GAAGNNNNNNTAC 2 cut(s) 1127, 1159
Bbv12I GWGCWC 2 cut(s) 696, 981
BbvI GCAGC 2 cut(s) 45, 1150
BccI CCATC 9 cut(s) 82, 353, 613, 727, 923, 995, 1021, 1196, 1409
BceAI ACGGC 1 cut(s) 716
BciT130I CCWGG 8 cut(s) 378, 423, 555, 570, 585, 807, 974, 1334
BciVI GTATCC 1 cut(s) 1205
BcnI CCSGG 3 cut(s) 206, 1364, 1375
BcoDI GTCTC 1 cut(s) 675
BfaI CTAG 4 cut(s) 711, 1148, 1307, 1443
BfuI GTATCC 1 cut(s) 1205
BisI GCNGC 3 cut(s) 34, 792, 1139
BlpI GCTNAGC 1 cut(s) 1447
BlsI GCNGC 3 cut(s) 35, 793, 1140
BmcAI AGTACT 1 cut(s) 1179
Bme18I GGWCC 5 cut(s) 335, 413, 1070, 1360, 1370
BmgT120I GGNCC 7 cut(s) 335, 413, 702, 1031, 1070, 1360, 1370
BmiI GGNNCC 7 cut(s) 203, 414, 1032, 1072, 1362, 1372, 1439
BmsI GCATC 1 cut(s) 1141
BmtI GCTAGC 1 cut(s) 714
BpmI CTGGAG 1 cut(s) 995
Bpu1102I GCTNAGC 1 cut(s) 1447
BpuEI CTTGAG 2 cut(s) 671, 965
BpuMI CCSGG 3 cut(s) 206, 1364, 1375
BsaJI CCNNGG 9 cut(s) 167, 302, 504, 568, 569, 583, 705, 739, 1373
BsaWI WCCGGW 2 cut(s) 195, 871
BsaXI ACNNNNNCTCC 2 cut(s) 968, 998
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 422, 1259
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 2 cut(s) 141, 907
Bse3DI GCAATG 1 cut(s) 85
BseBI CCWGG 8 cut(s) 378, 423, 555, 570, 585, 807, 974, 1334
BseDI CCNNGG 9 cut(s) 167, 302, 504, 568, 569, 583, 705, 739, 1373
BseGI GGATG 2 cut(s) 364, 1006
BseLI CCNNNNNNNGG 3 cut(s) 59, 422, 1259
BseMI GCAATG 1 cut(s) 85
BseNI ACTGG 2 cut(s) 141, 907
BseRI GAGGAG 1 cut(s) 534
BseSI GKGCMC 2 cut(s) 696, 761
BseXI GCAGC 2 cut(s) 45, 1150
BsgI GTGCAG 1 cut(s) 1194
BshFI GGCC 3 cut(s) 703, 791, 1033
BshNI GGYRCC 1 cut(s) 201
BshTI ACCGGT 1 cut(s) 195
BsiHKAI GWGCWC 2 cut(s) 696, 981
BsiSI CCGG 5 cut(s) 196, 206, 872, 1364, 1375
BslFI GGGAC 3 cut(s) 1056, 1346, 1356
BslI CCNNNNNNNGG 3 cut(s) 59, 422, 1259
BsmAI GTCTC 1 cut(s) 675
BsmFI GGGAC 3 cut(s) 1056, 1346, 1356
BsmI GAATGC 3 cut(s) 321, 941, 1189
BsnI GGCC 3 cut(s) 703, 791, 1033
Bsp1286I GDGCHC 3 cut(s) 696, 761, 981
Bsp143I GATC 2 cut(s) 382, 747
Bsp1720I GCTNAGC 1 cut(s) 1447
BspACI CCGC 2 cut(s) 273, 792
BspANI GGCC 3 cut(s) 703, 791, 1033
BspLI GGNNCC 7 cut(s) 203, 414, 1032, 1072, 1362, 1372, 1439
BspOI GCTAGC 1 cut(s) 714
BspPI GGATC 1 cut(s) 755
BspT107I GGYRCC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 85
BsrFI RCCGGY 1 cut(s) 195
BsrI ACTGG 2 cut(s) 141, 907
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 9 cut(s) 167, 302, 504, 568, 569, 583, 705, 739, 1373
BssMI GATC 2 cut(s) 382, 747
BssNAI GTATAC 1 cut(s) 865
BssT1I CCWWGG 3 cut(s) 504, 705, 739
Bst1107I GTATAC 1 cut(s) 865
Bst2UI CCWGG 8 cut(s) 378, 423, 555, 570, 585, 807, 974, 1334
Bst4CI ACNGT 2 cut(s) 235, 1159
Bst6I CTCTTC 3 cut(s) 423, 805, 1047
BstAPI GCANNNNNTGC 1 cut(s) 1160
BstC8I GCNNGC 2 cut(s) 712, 1173
BstDEI CTNAG 3 cut(s) 845, 896, 1447
BstDSI CCRYGG 1 cut(s) 167
BstENI CCTNNNNNAGG 1 cut(s) 420
BstF5I GGATG 2 cut(s) 364, 1006
BstKTI GATC 2 cut(s) 385, 750
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 2 cut(s) 382, 747
BstMWI GCNNNNNNNGC 8 cut(s) 172, 506, 700, 788, 1160, 1254, 1292, 1307
BstNI CCWGG 8 cut(s) 378, 423, 555, 570, 585, 807, 974, 1334
BstNSI RCATGY 1 cut(s) 913
BstSLI GKGCMC 2 cut(s) 696, 761
BstV1I GCAGC 2 cut(s) 45, 1150
BstZ17I GTATAC 1 cut(s) 865
BsuI GTATCC 1 cut(s) 1205
BsuRI GGCC 3 cut(s) 703, 791, 1033
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 2 cut(s) 364, 1006
BtsI GCAGTG 2 cut(s) 296, 789
BtsIMutI CAGTG 3 cut(s) 296, 789, 900
Cac8I GCNNGC 2 cut(s) 712, 1173
Cfr10I RCCGGY 1 cut(s) 195
Cfr13I GGNCC 7 cut(s) 335, 413, 702, 1031, 1070, 1360, 1370
CpoI CGGWCCG 1 cut(s) 335
CseI GACGC 2 cut(s) 724, 1292
Csp6I GTAC 2 cut(s) 1178, 1407
CspAI ACCGGT 1 cut(s) 195
CspI CGGWCCG 1 cut(s) 335
CviAII CATG 4 cut(s) 910, 1046, 1245, 1254
CviQI GTAC 2 cut(s) 1178, 1407
DdeI CTNAG 3 cut(s) 845, 896, 1447
DpnI GATC 2 cut(s) 384, 749
DpnII GATC 2 cut(s) 382, 747
Eam1104I CTCTTC 3 cut(s) 423, 805, 1047
EarI CTCTTC 3 cut(s) 423, 805, 1047
Ecl136II GAGCTC 1 cut(s) 979
Eco130I CCWWGG 3 cut(s) 504, 705, 739
Eco24I GRGCYC 1 cut(s) 981
Eco47I GGWCC 5 cut(s) 335, 413, 1070, 1360, 1370
Eco53kI GAGCTC 1 cut(s) 979
EcoICRI GAGCTC 1 cut(s) 979
EcoNI CCTNNNNNAGG 1 cut(s) 420
EcoO109I RGGNCCY 3 cut(s) 413, 1070, 1360
EcoRI GAATTC 1 cut(s) 363
EcoRII CCWGG 8 cut(s) 376, 421, 553, 568, 583, 805, 972, 1332
EcoT14I CCWWGG 3 cut(s) 504, 705, 739
EcoT38I GRGCYC 1 cut(s) 981
ErhI CCWWGG 3 cut(s) 504, 705, 739
FaeI CATG 4 cut(s) 913, 1049, 1248, 1257
FaqI GGGAC 3 cut(s) 1056, 1346, 1356
FatI CATG 4 cut(s) 909, 1045, 1244, 1253
FauI CCCGC 1 cut(s) 266
FauNDI CATATG 1 cut(s) 626
FblI GTMKAC 2 cut(s) 864, 1281
Fnu4HI GCNGC 3 cut(s) 34, 792, 1139
FokI GGATG 2 cut(s) 371, 1013
FriOI GRGCYC 1 cut(s) 981
Fsp4HI GCNGC 3 cut(s) 34, 792, 1139
FspBI CTAG 4 cut(s) 711, 1148, 1307, 1443
GluI GCNGC 3 cut(s) 34, 792, 1139
GsuI CTGGAG 1 cut(s) 995
HaeIII GGCC 3 cut(s) 703, 791, 1033
HapII CCGG 5 cut(s) 196, 206, 872, 1364, 1375
HgaI GACGC 2 cut(s) 724, 1292
Hin1II CATG 4 cut(s) 913, 1049, 1248, 1257
HincII GTYRAC 2 cut(s) 576, 1282
HindII GTYRAC 2 cut(s) 576, 1282
HindIII AAGCTT 1 cut(s) 179
HinfI GANTC 6 cut(s) 63, 564, 663, 841, 1051, 1086
HpaI GTTAAC 1 cut(s) 576
HpaII CCGG 5 cut(s) 196, 206, 872, 1364, 1375
HphI GGTGA 3 cut(s) 392, 917, 944
Hpy166II GTNNAC 4 cut(s) 576, 694, 865, 1282
Hpy188I TCNGA 5 cut(s) 335, 352, 480, 675, 1093
Hpy188III TCNNGA 5 cut(s) 312, 619, 764, 838, 1272
Hpy8I GTNNAC 4 cut(s) 576, 694, 865, 1282
Hpy99I CGWCG 2 cut(s) 1079, 1286
HpyAV CCTTC 5 cut(s) 335, 518, 878, 878, 1244
HpyCH4III ACNGT 2 cut(s) 235, 1159
HpyCH4V TGCA 7 cut(s) 405, 500, 694, 913, 1154, 1175, 1301
HpyF10VI GCNNNNNNNGC 8 cut(s) 172, 506, 700, 788, 1160, 1254, 1292, 1307
HpyF3I CTNAG 3 cut(s) 845, 896, 1447
Hsp92II CATG 4 cut(s) 913, 1049, 1248, 1257
KspAI GTTAAC 1 cut(s) 576
Kzo9I GATC 2 cut(s) 382, 747
LmnI GCTCC 5 cut(s) 128, 172, 887, 976, 1291
Lsp1109I GCAGC 2 cut(s) 45, 1150
LweI GCATC 1 cut(s) 1141
MaeI CTAG 4 cut(s) 711, 1148, 1307, 1443
MaeIII GTNAC 2 cut(s) 830, 905
MalI GATC 2 cut(s) 384, 749
MboI GATC 2 cut(s) 382, 747
MboII GAAGA 8 cut(s) 58, 87, 111, 377, 410, 822, 1034, 1275
MfeI CAATTG 1 cut(s) 826
MhlI GDGCHC 3 cut(s) 696, 761, 981
MluCI AATT 8 cut(s) 329, 363, 801, 826, 990, 1205, 1266, 1420
MlyI GAGTC 1 cut(s) 672
MmeI TCCRAC 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 1267
MseI TTAA 1 cut(s) 575
MspA1I CMGCKG 1 cut(s) 273
MspI CCGG 5 cut(s) 196, 206, 872, 1364, 1375
MunI CAATTG 1 cut(s) 826
Mva1269I GAATGC 3 cut(s) 321, 941, 1189
MvaI CCWGG 8 cut(s) 378, 423, 555, 570, 585, 807, 974, 1334
MwoI GCNNNNNNNGC 8 cut(s) 172, 506, 700, 788, 1160, 1254, 1292, 1307
NciI CCSGG 3 cut(s) 206, 1364, 1375
NdeI CATATG 1 cut(s) 626
NdeII GATC 2 cut(s) 382, 747
NheI GCTAGC 1 cut(s) 710
NlaIII CATG 4 cut(s) 913, 1049, 1248, 1257
NlaIV GGNNCC 7 cut(s) 203, 414, 1032, 1072, 1362, 1372, 1439
NmeAIII GCCGAG 1 cut(s) 327
NmuCI GTSAC 2 cut(s) 830, 905
NspI RCATGY 1 cut(s) 913
PasI CCCWGGG 1 cut(s) 569
PctI GAATGC 3 cut(s) 321, 941, 1189
PdmI GAANNNNTTC 1 cut(s) 1267
PfeI GAWTC 5 cut(s) 63, 564, 841, 1051, 1086
PfoI TCCNGGA 3 cut(s) 421, 805, 972
PinAI ACCGGT 1 cut(s) 195
PkrI GCNGC 3 cut(s) 35, 793, 1140
PleI GAGTC 1 cut(s) 671
PpsI GAGTC 1 cut(s) 671
PpuMI RGGWCCY 3 cut(s) 413, 1070, 1360
PsiI TTATAA 1 cut(s) 995
Psp124BI GAGCTC 1 cut(s) 981
Psp5II RGGWCCY 3 cut(s) 413, 1070, 1360
Psp6I CCWGG 8 cut(s) 376, 421, 553, 568, 583, 805, 972, 1332
PspGI CCWGG 8 cut(s) 376, 421, 553, 568, 583, 805, 972, 1332
PspN4I GGNNCC 7 cut(s) 203, 414, 1032, 1072, 1362, 1372, 1439
PspPI GGNCC 7 cut(s) 335, 413, 702, 1031, 1070, 1360, 1370
PspPPI RGGWCCY 3 cut(s) 413, 1070, 1360
RsaI GTAC 2 cut(s) 1179, 1408
RsaNI GTAC 2 cut(s) 1178, 1407
Rsr2I CGGWCCG 1 cut(s) 335
RsrII CGGWCCG 1 cut(s) 335
SacI GAGCTC 1 cut(s) 981
SalI GTCGAC 1 cut(s) 1280
SaqAI TTAA 1 cut(s) 575
SatI GCNGC 3 cut(s) 34, 792, 1139
Sau3AI GATC 2 cut(s) 382, 747
Sau96I GGNCC 7 cut(s) 335, 413, 702, 1031, 1070, 1360, 1370
ScaI AGTACT 1 cut(s) 1179
SchI GAGTC 1 cut(s) 672
SduI GDGCHC 3 cut(s) 696, 761, 981
SfaNI GCATC 1 cut(s) 1141
SinI GGWCC 5 cut(s) 335, 413, 1070, 1360, 1370
SmlI CTYRAG 2 cut(s) 650, 980
SmoI CTYRAG 2 cut(s) 650, 980
Sse9I AATT 8 cut(s) 329, 363, 801, 826, 990, 1205, 1266, 1420
SsiI CCGC 2 cut(s) 273, 792
SspMI CTAG 4 cut(s) 711, 1148, 1307, 1443
SstI GAGCTC 1 cut(s) 981
StyI CCWWGG 3 cut(s) 504, 705, 739
TaaI ACNGT 2 cut(s) 235, 1159
TaqI TCGA 6 cut(s) 250, 453, 1077, 1191, 1281, 1342
TaqII GACCGA 1 cut(s) 352
TasI AATT 8 cut(s) 329, 363, 801, 826, 990, 1205, 1266, 1420
TatI WGTACW 2 cut(s) 1177, 1406
TauI GCSGC 1 cut(s) 794
TfiI GAWTC 5 cut(s) 63, 564, 841, 1051, 1086
Tru1I TTAA 1 cut(s) 575
Tru9I TTAA 1 cut(s) 575
TscAI CASTG 3 cut(s) 303, 789, 907
TseFI GTSAC 2 cut(s) 830, 905
TseI GCWGC 2 cut(s) 33, 1138
Tsp45I GTSAC 2 cut(s) 830, 905
TspDTI ATGAA 5 cut(s) 42, 444, 868, 1022, 1034
TspGWI ACGGA 1 cut(s) 626
TspRI CASTG 3 cut(s) 303, 789, 907
VneI GTGCAC 1 cut(s) 692
VpaK11BI GGWCC 5 cut(s) 335, 413, 1070, 1360, 1370
XagI CCTNNNNNAGG 1 cut(s) 420
XapI RAATTY 4 cut(s) 363, 1205, 1266, 1420
XceI RCATGY 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 1 cut(s) 429
XmiI GTMKAC 2 cut(s) 864, 1281
XmnI GAANNNNTTC 1 cut(s) 1267
XspI CTAG 4 cut(s) 711, 1148, 1307, 1443
ZrmI AGTACT 1 cut(s) 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.