FvH4_1g17390

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003336
Physical Location & Seq
Forward (+)
10069222 .. 10072785
3564 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g17390.t7

Sequence Viewer

Length: 1356 bp
ATGCCAATCCTTCAATTATTAGTAGTTCCGATTAACTTGAGCAGCAAACCATGGGAGCAAAAGCTACCTCTTCACAACCGGTGGCACCCAGACATACCCCCGGTGGCTGAGGTCAAAACTGGTGAGTTGTTTAGAGTAGAGATGGTAGACTGGACTGGAGGTGCTATTAACGATGATGACTCTGCACTAGACATAAAAACCGTAGACCTCTCCACTGTTCACTATCTCAGCGGACCAATTAGAATTTCGGACAAGGATGGAATCCCAGCACAGCCAGGCGATCTTCTGGTAGTTGAGATATGCAACTTGGGTCCTCTTCCAGGGGATGAATGGGGTTTCACAGCAACATTTGACAGAGAAAATGGAGGGGGTTTTCTGACTGACCATTTTCCTTGTGCTACAAAAGCTATTTGGTATTTTGAAGGAATATATGCCTACTCTCCTCAGATACCAGGAGTGAGATTCCCAGGTTTAACACACCCTGGAATAGTTGGTACAGCACCATCAATGGAACTCCTGAACATATGGAATAAGAGGGAGAGAGAACTTGAGGAAAATGGAGTTGAGTCTCTGAAACTATGCGAGGTTGTGCACCAACGGCCACTGGCTAGCCTACCATCGACAAAAGGTTGCGTCCTTGGAGGGATCAAAGAAGGCACTCCTGAATGGGAAAAGATAGCCGTGGAGGCTGCAAGAACAATTCCAGGAAGAGAAAATGGTGGCAATTGTGACATCAAGAATCTTAGTAGAGGTTCAAAGGTGTACCTTCCGGTATTTGTTGAAGGAGCAAATCTTAGCACTGGTGACATGCACTTTTCCCAGGGAGATGGTGAAATATCATTCTGTGGTGCAATTGAGATGAGTGGTTTCCTAGAGCTCAAGTGTGATATTATAAGGGATGGAATGAAAGAATACCTTACACCGATGGGGCCAACTCCTCTTCATGTGAATCCAATCTTTGAGATAGGCCCTGTCGAGCCAAGATTCTCAGAATGGTTGGTATTTGAGGGCATCAGTGTTGATGAGAGTGGGAAGCAGCATTACCTAGATGCAACAGTTGCTTACAAGCGCGCAGTACTCAATGCCATCGACTACCTCTCTAAATTTGGATACTCCAAAGAACAGAGCTACCTTCTGCTGTCATGCTGTCCATGTGAGGGAAGAATTTCGGGAATAGTCGACGCTCCCAATGCTGTTGCAACCCTAGCAATCCCAATAGCTATCTTTGACCAGGATATTCGACCAAAAACGAACAAGGTGCCGGTTGGTCCCCGGATAGTGAGGACACCAGATGTCCTAAAATGTACTTATGATGGAAATTTGCCAACGACAAGGAACCCTAGCGCAGCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

452

Amino Acids

49.4

Weight (kDa)

5.04

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 22 - 366 1.1e-144 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 893
AccB1I GGYRCC 2 cut(s) 84, 1258
AccI GTMKAC 3 cut(s) 147, 204, 1179
AccII CGCG 1 cut(s) 1071
AciI CCGC 1 cut(s) 231
AclWI GGATC 1 cut(s) 653
AcoI YGGCCR 1 cut(s) 599
AcsI RAATTY 4 cut(s) 243, 1103, 1164, 1318
AfaI GTAC 4 cut(s) 496, 764, 1077, 1306
AfiI CCNNNNNNNGG 2 cut(s) 320, 1157
AgeI ACCGGT 1 cut(s) 78
AgsI TTSAA 4 cut(s) 14, 422, 756, 782
AjnI CCWGG 8 cut(s) 274, 319, 451, 466, 481, 703, 819, 1230
AjuI GAANNNNNNNTTGG 2 cut(s) 229, 261
AluBI AGCT 5 cut(s) 64, 407, 877, 1128, 1220
AluI AGCT 5 cut(s) 64, 407, 877, 1128, 1220
Alw21I GWGCWC 2 cut(s) 594, 879
Alw26I GTCTC 1 cut(s) 573
Alw44I GTGCAC 1 cut(s) 590
AlwI GGATC 1 cut(s) 653
AoxI GGCC 3 cut(s) 599, 929, 967
ApaLI GTGCAC 1 cut(s) 590
ApeKI GCWGC 4 cut(s) 42, 689, 1036, 1346
ApoI RAATTY 4 cut(s) 243, 1103, 1164, 1318
AsiGI ACCGGT 1 cut(s) 78
Asp700I GAANNNNTTC 1 cut(s) 1165
AspLEI GCGC 3 cut(s) 1071, 1073, 1346
AspS9I GGNCC 5 cut(s) 233, 311, 929, 968, 1268
AsuC2I CCSGG 2 cut(s) 101, 1273
AsuHPI GGTGA 3 cut(s) 134, 815, 842
AsuNHI GCTAGC 1 cut(s) 608
AvaII GGWCC 3 cut(s) 233, 311, 1268
BaeGI GKGCMC 1 cut(s) 594
BanI GGYRCC 2 cut(s) 84, 1258
BanII GRGCYC 1 cut(s) 879
BarI GAAGNNNNNNTAC 2 cut(s) 1025, 1057
Bbv12I GWGCWC 2 cut(s) 594, 879
BbvCI CCTCAGC 1 cut(s) 108
BbvI GCAGC 3 cut(s) 54, 676, 1048
BccI CCATC 9 cut(s) 136, 251, 511, 625, 821, 893, 919, 1094, 1307
BceAI ACGGC 2 cut(s) 614, 665
BcgI CGANNNNNNTGC 2 cut(s) 1240, 1274
BciT130I CCWGG 8 cut(s) 276, 321, 453, 468, 483, 705, 821, 1232
BciVI GTATCC 1 cut(s) 1103
BcnI CCSGG 2 cut(s) 101, 1273
BcoDI GTCTC 1 cut(s) 573
BfaI CTAG 6 cut(s) 188, 609, 872, 1046, 1205, 1341
BfuI GTATCC 1 cut(s) 1103
BglI GCCNNNNNGGC 1 cut(s) 686
BisI GCNGC 4 cut(s) 43, 690, 1037, 1347
BlsI GCNGC 4 cut(s) 44, 691, 1038, 1348
BmcAI AGTACT 1 cut(s) 1077
Bme18I GGWCC 3 cut(s) 233, 311, 1268
BmgT120I GGNCC 5 cut(s) 233, 311, 929, 968, 1268
BmiI GGNNCC 6 cut(s) 86, 312, 930, 1260, 1270, 1337
BmsI GCATC 2 cut(s) 1020, 1039
BmtI GCTAGC 1 cut(s) 612
BpmI CTGGAG 1 cut(s) 177
Bpu10I CCTNAGC 1 cut(s) 108
BpuEI CTTGAG 3 cut(s) 58, 569, 863
BpuMI CCSGG 2 cut(s) 101, 1273
BsaWI WCCGGW 2 cut(s) 78, 769
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 1157
Bse118I RCCGGY 2 cut(s) 78, 1261
Bse1I ACTGG 5 cut(s) 124, 155, 160, 609, 805
BseBI CCWGG 8 cut(s) 276, 321, 453, 468, 483, 705, 821, 1232
BseGI GGATG 3 cut(s) 262, 331, 904
BseLI CCNNNNNNNGG 2 cut(s) 320, 1157
BseMII CTCAG 4 cut(s) 99, 241, 458, 1002
BseNI ACTGG 5 cut(s) 124, 155, 160, 609, 805
BsePI GCGCGC 1 cut(s) 1069
BseRI GAGGAG 2 cut(s) 432, 927
BseSI GKGCMC 1 cut(s) 594
BseXI GCAGC 3 cut(s) 54, 676, 1048
BseYI CCCAGC 1 cut(s) 265
BsgI GTGCAG 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 1071
BshFI GGCC 3 cut(s) 601, 931, 969
BshNI GGYRCC 2 cut(s) 84, 1258
BshTI ACCGGT 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 594, 879
BsiSI CCGG 5 cut(s) 79, 101, 770, 1262, 1273
BslFI GGGAC 1 cut(s) 1254
BslI CCNNNNNNNGG 2 cut(s) 320, 1157
BsmAI GTCTC 1 cut(s) 573
BsmFI GGGAC 1 cut(s) 1254
BsnI GGCC 3 cut(s) 601, 931, 969
Bsp1286I GDGCHC 2 cut(s) 594, 879
Bsp143I GATC 2 cut(s) 280, 645
Bsp19I CCATGG 1 cut(s) 50
BspACI CCGC 1 cut(s) 231
BspANI GGCC 3 cut(s) 601, 931, 969
BspCNI CTCAG 4 cut(s) 100, 240, 457, 1001
BspFNI CGCG 1 cut(s) 1071
BspLI GGNNCC 6 cut(s) 86, 312, 930, 1260, 1270, 1337
BspOI GCTAGC 1 cut(s) 612
BspPI GGATC 1 cut(s) 653
BspT107I GGYRCC 2 cut(s) 84, 1258
BsrFI RCCGGY 2 cut(s) 78, 1261
BsrI ACTGG 5 cut(s) 124, 155, 160, 609, 805
BssAI RCCGGY 2 cut(s) 78, 1261
BssHII GCGCGC 1 cut(s) 1069
BssMI GATC 2 cut(s) 280, 645
BssT1I CCWWGG 2 cut(s) 50, 637
Bst2UI CCWGG 8 cut(s) 276, 321, 453, 468, 483, 705, 821, 1232
Bst4CI ACNGT 3 cut(s) 202, 217, 1057
Bst6I CTCTTC 4 cut(s) 75, 321, 703, 945
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstC8I GCNNGC 2 cut(s) 610, 1071
BstDEI CTNAG 6 cut(s) 108, 227, 444, 743, 794, 988
BstDSI CCRYGG 2 cut(s) 50, 681
BstENI CCTNNNNNAGG 1 cut(s) 318
BstF5I GGATG 3 cut(s) 262, 331, 904
BstFNI CGCG 1 cut(s) 1071
BstHHI GCGC 3 cut(s) 1071, 1073, 1346
BstKTI GATC 2 cut(s) 283, 648
BstMAI GTCTC 1 cut(s) 573
BstMBI GATC 2 cut(s) 280, 645
BstMWI GCNNNNNNNGC 6 cut(s) 404, 598, 686, 1058, 1190, 1205
BstNI CCWGG 8 cut(s) 276, 321, 453, 468, 483, 705, 821, 1232
BstNSI RCATGY 1 cut(s) 811
BstSLI GKGCMC 1 cut(s) 594
BstUI CGCG 1 cut(s) 1071
BstV1I GCAGC 3 cut(s) 54, 676, 1048
BstXI CCANNNNNNTGG 1 cut(s) 827
BsuI GTATCC 1 cut(s) 1103
BsuRI GGCC 3 cut(s) 601, 931, 969
BtgI CCRYGG 2 cut(s) 50, 681
BtsCI GGATG 3 cut(s) 262, 331, 904
BtsIMutI CAGTG 4 cut(s) 213, 602, 798, 1021
Cac8I GCNNGC 2 cut(s) 610, 1071
CfoI GCGC 3 cut(s) 1071, 1073, 1346
Cfr10I RCCGGY 2 cut(s) 78, 1261
Cfr13I GGNCC 5 cut(s) 233, 311, 929, 968, 1268
CseI GACGC 2 cut(s) 622, 1190
Csp6I GTAC 4 cut(s) 495, 763, 1076, 1305
CspAI ACCGGT 1 cut(s) 78
CviAII CATG 5 cut(s) 51, 808, 944, 1143, 1152
CviQI GTAC 4 cut(s) 495, 763, 1076, 1305
DdeI CTNAG 6 cut(s) 108, 227, 444, 743, 794, 988
DpnI GATC 2 cut(s) 282, 647
DpnII GATC 2 cut(s) 280, 645
EaeI YGGCCR 1 cut(s) 599
Eam1104I CTCTTC 4 cut(s) 75, 321, 703, 945
EarI CTCTTC 4 cut(s) 75, 321, 703, 945
Ecl136II GAGCTC 1 cut(s) 877
Eco130I CCWWGG 2 cut(s) 50, 637
Eco24I GRGCYC 1 cut(s) 879
Eco47I GGWCC 3 cut(s) 233, 311, 1268
Eco53kI GAGCTC 1 cut(s) 877
EcoICRI GAGCTC 1 cut(s) 877
EcoNI CCTNNNNNAGG 1 cut(s) 318
EcoO109I RGGNCCY 2 cut(s) 311, 968
EcoRII CCWGG 8 cut(s) 274, 319, 451, 466, 481, 703, 819, 1230
EcoT14I CCWWGG 2 cut(s) 50, 637
EcoT38I GRGCYC 1 cut(s) 879
ErhI CCWWGG 2 cut(s) 50, 637
FaeI CATG 5 cut(s) 54, 811, 947, 1146, 1155
FalI AAGNNNNNCTT 2 cut(s) 900, 932
FaqI GGGAC 1 cut(s) 1254
FatI CATG 5 cut(s) 50, 807, 943, 1142, 1151
FauNDI CATATG 1 cut(s) 524
FblI GTMKAC 3 cut(s) 147, 204, 1179
Fnu4HI GCNGC 4 cut(s) 43, 690, 1037, 1347
FokI GGATG 3 cut(s) 269, 338, 911
FriOI GRGCYC 1 cut(s) 879
Fsp4HI GCNGC 4 cut(s) 43, 690, 1037, 1347
FspBI CTAG 6 cut(s) 188, 609, 872, 1046, 1205, 1341
GlaI GCGC 3 cut(s) 1070, 1072, 1345
GluI GCNGC 4 cut(s) 43, 690, 1037, 1347
GsaI CCCAGC 1 cut(s) 269
GsuI CTGGAG 1 cut(s) 177
HaeIII GGCC 3 cut(s) 601, 931, 969
HapII CCGG 5 cut(s) 79, 101, 770, 1262, 1273
HgaI GACGC 2 cut(s) 622, 1190
HhaI GCGC 3 cut(s) 1071, 1073, 1346
Hin1II CATG 5 cut(s) 54, 811, 947, 1146, 1155
Hin6I GCGC 3 cut(s) 1069, 1071, 1344
HinP1I GCGC 3 cut(s) 1069, 1071, 1344
HincII GTYRAC 1 cut(s) 1180
HindII GTYRAC 1 cut(s) 1180
HinfI GANTC 7 cut(s) 179, 261, 462, 566, 739, 949, 984
HpaII CCGG 5 cut(s) 79, 101, 770, 1262, 1273
HphI GGTGA 3 cut(s) 134, 815, 842
Hpy166II GTNNAC 6 cut(s) 148, 205, 220, 592, 763, 1180
Hpy188I TCNGA 6 cut(s) 30, 250, 378, 447, 573, 991
Hpy188III TCNNGA 4 cut(s) 517, 662, 736, 1170
Hpy8I GTNNAC 6 cut(s) 148, 205, 220, 592, 763, 1180
Hpy99I CGWCG 1 cut(s) 1184
HpyAV CCTTC 6 cut(s) 20, 416, 647, 776, 776, 1142
HpyCH4III ACNGT 3 cut(s) 202, 217, 1057
HpyCH4V TGCA 8 cut(s) 185, 303, 592, 692, 811, 851, 1052, 1199
HpyF10VI GCNNNNNNNGC 6 cut(s) 404, 598, 686, 1058, 1190, 1205
HpyF3I CTNAG 6 cut(s) 108, 227, 444, 743, 794, 988
Hsp92II CATG 5 cut(s) 54, 811, 947, 1146, 1155
HspAI GCGC 3 cut(s) 1069, 1071, 1344
Kzo9I GATC 2 cut(s) 280, 645
LmnI GCTCC 3 cut(s) 55, 785, 1189
Lsp1109I GCAGC 3 cut(s) 54, 676, 1048
LweI GCATC 2 cut(s) 1020, 1039
MaeI CTAG 6 cut(s) 188, 609, 872, 1046, 1205, 1341
MaeIII GTNAC 2 cut(s) 728, 803
MalI GATC 2 cut(s) 282, 647
MboI GATC 2 cut(s) 280, 645
MboII GAAGA 6 cut(s) 62, 275, 308, 720, 932, 1173
MfeI CAATTG 2 cut(s) 724, 852
MhlI GDGCHC 2 cut(s) 594, 879
MluCI AATT 9 cut(s) 14, 237, 243, 699, 724, 852, 1103, 1164, 1318
MlyI GAGTC 2 cut(s) 173, 575
MroXI GAANNNNTTC 1 cut(s) 1165
MseI TTAA 3 cut(s) 33, 168, 473
MspA1I CMGCKG 1 cut(s) 231
MspI CCGG 5 cut(s) 79, 101, 770, 1262, 1273
MunI CAATTG 2 cut(s) 724, 852
MvaI CCWGG 8 cut(s) 276, 321, 453, 468, 483, 705, 821, 1232
MvnI CGCG 1 cut(s) 1071
MwoI GCNNNNNNNGC 6 cut(s) 404, 598, 686, 1058, 1190, 1205
NciI CCSGG 2 cut(s) 101, 1273
NcoI CCATGG 1 cut(s) 50
NdeI CATATG 1 cut(s) 524
NdeII GATC 2 cut(s) 280, 645
NheI GCTAGC 1 cut(s) 608
NlaIII CATG 5 cut(s) 54, 811, 947, 1146, 1155
NlaIV GGNNCC 6 cut(s) 86, 312, 930, 1260, 1270, 1337
NmuCI GTSAC 2 cut(s) 728, 803
NspI RCATGY 1 cut(s) 811
PasI CCCWGGG 1 cut(s) 820
PauI GCGCGC 1 cut(s) 1069
PdmI GAANNNNTTC 1 cut(s) 1165
PfeI GAWTC 5 cut(s) 261, 462, 739, 949, 984
PfoI TCCNGGA 1 cut(s) 703
PinAI ACCGGT 1 cut(s) 78
PkrI GCNGC 4 cut(s) 44, 691, 1038, 1348
PleI GAGTC 2 cut(s) 173, 574
PpsI GAGTC 2 cut(s) 173, 574
PpuMI RGGWCCY 1 cut(s) 311
PsiI TTATAA 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 879
Psp5II RGGWCCY 1 cut(s) 311
Psp6I CCWGG 8 cut(s) 274, 319, 451, 466, 481, 703, 819, 1230
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 8 cut(s) 274, 319, 451, 466, 481, 703, 819, 1230
PspN4I GGNNCC 6 cut(s) 86, 312, 930, 1260, 1270, 1337
PspPI GGNCC 5 cut(s) 233, 311, 929, 968, 1268
PspPPI RGGWCCY 1 cut(s) 311
PteI GCGCGC 1 cut(s) 1069
RsaI GTAC 4 cut(s) 496, 764, 1077, 1306
RsaNI GTAC 4 cut(s) 495, 763, 1076, 1305
SacI GAGCTC 1 cut(s) 879
SalI GTCGAC 1 cut(s) 1178
SaqAI TTAA 3 cut(s) 33, 168, 473
SatI GCNGC 4 cut(s) 43, 690, 1037, 1347
Sau3AI GATC 2 cut(s) 280, 645
Sau96I GGNCC 5 cut(s) 233, 311, 929, 968, 1268
ScaI AGTACT 1 cut(s) 1077
SchI GAGTC 2 cut(s) 173, 575
SduI GDGCHC 2 cut(s) 594, 879
SfaNI GCATC 2 cut(s) 1020, 1039
SinI GGWCC 3 cut(s) 233, 311, 1268
SmlI CTYRAG 3 cut(s) 37, 548, 878
SmoI CTYRAG 3 cut(s) 37, 548, 878
Sse9I AATT 9 cut(s) 14, 237, 243, 699, 724, 852, 1103, 1164, 1318
SsiI CCGC 1 cut(s) 231
SspMI CTAG 6 cut(s) 188, 609, 872, 1046, 1205, 1341
SstI GAGCTC 1 cut(s) 879
StyI CCWWGG 2 cut(s) 50, 637
TaaI ACNGT 3 cut(s) 202, 217, 1057
TaqI TCGA 5 cut(s) 620, 975, 1089, 1179, 1240
TasI AATT 9 cut(s) 14, 237, 243, 699, 724, 852, 1103, 1164, 1318
TatI WGTACW 2 cut(s) 1075, 1304
TfiI GAWTC 5 cut(s) 261, 462, 739, 949, 984
Tru1I TTAA 3 cut(s) 33, 168, 473
Tru9I TTAA 3 cut(s) 33, 168, 473
TscAI CASTG 4 cut(s) 220, 609, 805, 1021
TseFI GTSAC 2 cut(s) 728, 803
TseI GCWGC 4 cut(s) 42, 689, 1036, 1346
Tsp45I GTSAC 2 cut(s) 728, 803
TspDTI ATGAA 3 cut(s) 342, 920, 932
TspRI CASTG 4 cut(s) 220, 609, 805, 1021
VneI GTGCAC 1 cut(s) 590
VpaK11BI GGWCC 3 cut(s) 233, 311, 1268
XagI CCTNNNNNAGG 1 cut(s) 318
XapI RAATTY 4 cut(s) 243, 1103, 1164, 1318
XceI RCATGY 1 cut(s) 811
XcmI CCANNNNNNNNNTGG 1 cut(s) 327
XmiI GTMKAC 3 cut(s) 147, 204, 1179
XmnI GAANNNNTTC 1 cut(s) 1165
XspI CTAG 6 cut(s) 188, 609, 872, 1046, 1205, 1341
ZrmI AGTACT 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.