Rorug02G0145100

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003336
Physical Location & Seq
Reverse (-)
12740232 .. 12742070
1839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0145100.1

Sequence Viewer

Length: 1164 bp
ATGGAGACTCCACTGAGAATCCTCAGACTCAACCGCCACCTCAAAACGACGCCGTCCGCTCTTTTCTTCTCCCCCAAATTTCACCCACTTGCATTTCCCAATTTCCGGCTAAACGACGCCGTAAAGAGAACCCCTTTTGCTCTTTCTCACTCCTCCTCTTCCTGGAATCCAAACCCTTTCAAATTCAACCCCTCAATTTCCCAATCTCCATTTCCGATCACCAATTTCAACCACTCCCTGTCGTACCAAAACGACGCCGGTTTCAGATGGAACCGAGCGGTCCACAGCGGCATTGACGGAAATGTGGGATCTTTGGGGGCCCAAAAGCGGGAAGTGACGGTGGTGTTGCTGGGCTGGCTTGGGGGTCAGACCAAGCACCTGAAGAGGTACGTGGAGTGGTACAATTCCCGAGGCTTTCACGCTGTGACGTTTGTTGTGGACGTCAAGGAGGTGCTCTGGTTTGATTTGGGCCGCCGGGTTGAGAAGCGGGTCGCGGAGCTGGCCCATGAGCTGGCTTCGTGGGTTGAGGGCGGCGGAGAACGGTGTTTGGTGTTTCACACTTTTAGCAATACGGGTTGGTTTGTATATGGTGCAATACTTGAAATTTGGCTGGATAGACCAGACTTGATCAAGAAGATTAAAGGGTGTATTGTTGATTCAGGAGCTGGAGCCCCTTTTGATCCTAAGGTGTGGGCAGCAGGATTCTCTACTGCGATTATGAAGAAACGCAGCTCTTTAGCAAAACCTGCGGTTGAGACTAAAGAATTAAATGCCTCTGGAGGTGCAGCGAGCTTGTCAAAGGTGCAAGAAGAGGAACCTCCAGCAATTGAAACCATGCTTCTGTTGGTATTAGAGAAGCTGTTCTCACTCATTCTGAAGTTGCCTGATGTGGATAAGAGGTTAACCAAGGTAGTTTCTGTCCTCTCGACAAACCCTCCTCATTGTCCACAGCTTTACCTGTACAGTACAGGTGATAAAGTCGTCCCACACCAATCCATCGAATCCTTCATCGCAGAGCAAAGGCAGATAGGTAGACCCGTGAAGTCCTTCAATTTTGGCACATCTCCTCACGTCGACCATTACAGGACTTTTCCCAACATATACTCTCAAGAGCTTAACAATTTCTTGAAAGAATGCTTGGCTACAACAGTTAAGCAGACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

387

Amino Acids

43.49

Weight (kDa)

9.48

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 113 - 376 9.9e-63 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 754
AccB7I CCANNNNNTGG 1 cut(s) 511
AccBSI CCGCTC 2 cut(s) 59, 278
AccI GTMKAC 2 cut(s) 1033, 1074
AccII CGCG 1 cut(s) 494
AclWI GGATC 2 cut(s) 316, 674
AcsI RAATTY 3 cut(s) 77, 182, 603
AcuI CTGAAG 2 cut(s) 401, 896
AcyI GRCGYC 4 cut(s) 50, 117, 255, 441
AdeI CACNNNGTG 1 cut(s) 424
AfaI GTAC 5 cut(s) 245, 389, 401, 962, 967
AfiI CCNNNNNNNGG 5 cut(s) 105, 162, 327, 328, 511
AgsI TTSAA 7 cut(s) 181, 187, 229, 602, 830, 1051, 1129
AjiI CACGTC 1 cut(s) 1072
AjnI CCWGG 1 cut(s) 161
AjuI GAANNNNNNNTTGG 4 cut(s) 195, 227, 1121, 1153
AluBI AGCT 8 cut(s) 499, 511, 665, 732, 792, 859, 952, 1114
AluI AGCT 8 cut(s) 499, 511, 665, 732, 792, 859, 952, 1114
Alw21I GWGCWC 1 cut(s) 456
Alw26I GTCTC 1 cut(s) 749
AlwI GGATC 2 cut(s) 316, 674
AlwNI CAGNNNCTG 1 cut(s) 665
Ama87I CYCGRG 1 cut(s) 408
AoxI GGCC 3 cut(s) 318, 469, 501
ApaI GGGCCC 1 cut(s) 322
ApeKI GCWGC 3 cut(s) 695, 729, 785
ApoI RAATTY 3 cut(s) 77, 182, 603
Asp700I GAANNNNTTC 2 cut(s) 860, 1046
AspS9I GGNCC 5 cut(s) 280, 318, 319, 469, 502
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 3 cut(s) 74, 211, 983
AvaI CYCGRG 1 cut(s) 408
AvaII GGWCC 1 cut(s) 280
AxyI CCTNAGG 1 cut(s) 684
BaeGI GKGCMC 1 cut(s) 322
BanII GRGCYC 2 cut(s) 322, 673
Bbv12I GWGCWC 1 cut(s) 456
BbvI GCAGC 3 cut(s) 707, 741, 797
BccI CCATC 2 cut(s) 261, 1004
BceAI ACGGC 2 cut(s) 37, 104
BciT130I CCWGG 1 cut(s) 163
BclI TGATCA 1 cut(s) 627
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 1 cut(s) 749
BfuAI ACCTGC 1 cut(s) 754
BisI GCNGC 6 cut(s) 289, 472, 532, 696, 730, 786
BlsI GCNGC 6 cut(s) 290, 473, 533, 697, 731, 787
Bme1390I CCNGG 2 cut(s) 163, 476
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 1 cut(s) 408
BmgBI CACGTC 1 cut(s) 1072
BmgT120I GGNCC 5 cut(s) 280, 318, 319, 469, 502
BmiI GGNNCC 5 cut(s) 272, 319, 320, 670, 816
BmrFI CCNGG 2 cut(s) 163, 476
BpmI CTGGAG 3 cut(s) 687, 798, 804
BpuEI CTTGAG 1 cut(s) 1092
BpuMI CCSGG 1 cut(s) 476
BsaAI YACGTR 1 cut(s) 391
BsaHI GRCGYC 4 cut(s) 50, 117, 255, 441
BsaJI CCNNGG 2 cut(s) 409, 906
BsaXI ACNNNNNCTCC 2 cut(s) 919, 949
Bsc4I CCNNNNNNNGG 5 cut(s) 105, 162, 327, 328, 511
Bse118I RCCGGY 1 cut(s) 257
Bse21I CCTNAGG 1 cut(s) 684
BseBI CCWGG 1 cut(s) 163
BseDI CCNNGG 2 cut(s) 409, 906
BseLI CCNNNNNNNGG 5 cut(s) 105, 162, 327, 328, 511
BseMII CTCAG 2 cut(s) 5, 37
BseRI GAGGAG 4 cut(s) 142, 145, 927, 1056
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 3 cut(s) 707, 741, 797
BseYI CCCAGC 1 cut(s) 349
BsgI GTGCAG 1 cut(s) 804
Bsh1236I CGCG 1 cut(s) 494
BshFI GGCC 3 cut(s) 320, 471, 503
BsiHKAI GWGCWC 1 cut(s) 456
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 3 cut(s) 106, 258, 475
BslFI GGGAC 1 cut(s) 968
BslI CCNNNNNNNGG 5 cut(s) 105, 162, 327, 328, 511
BsmAI GTCTC 1 cut(s) 749
BsmFI GGGAC 1 cut(s) 968
BsmI GAATGC 1 cut(s) 1139
BsnI GGCC 3 cut(s) 320, 471, 503
BsoBI CYCGRG 1 cut(s) 408
Bsp120I GGGCCC 1 cut(s) 318
Bsp1286I GDGCHC 3 cut(s) 322, 456, 673
Bsp1407I TGTACA 1 cut(s) 960
Bsp143I GATC 4 cut(s) 216, 308, 627, 679
BspANI GGCC 3 cut(s) 320, 471, 503
BspCNI CTCAG 2 cut(s) 6, 36
BspFNI CGCG 1 cut(s) 494
BspLI GGNNCC 5 cut(s) 272, 319, 320, 670, 816
BspMI ACCTGC 1 cut(s) 754
BspPI GGATC 2 cut(s) 316, 674
BsrBI CCGCTC 2 cut(s) 59, 278
BsrFI RCCGGY 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 960
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 409, 906
BssMI GATC 4 cut(s) 216, 308, 627, 679
BssNI GRCGYC 4 cut(s) 50, 117, 255, 441
BssT1I CCWWGG 1 cut(s) 906
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 4 cut(s) 340, 543, 965, 1150
Bst6I CTCTTC 3 cut(s) 163, 377, 804
BstACI GRCGYC 4 cut(s) 50, 117, 255, 441
BstAPI GCANNNNNTGC 1 cut(s) 746
BstAUI TGTACA 1 cut(s) 960
BstBAI YACGTR 1 cut(s) 391
BstC8I GCNNGC 4 cut(s) 356, 501, 513, 790
BstDEI CTNAG 3 cut(s) 14, 23, 684
BstFNI CGCG 1 cut(s) 494
BstKTI GATC 4 cut(s) 219, 311, 630, 682
BstMAI GTCTC 1 cut(s) 749
BstMBI GATC 4 cut(s) 216, 308, 627, 679
BstMWI GCNNNNNNNGC 3 cut(s) 355, 500, 746
BstNI CCWGG 1 cut(s) 163
BstSCI CCNGG 2 cut(s) 161, 474
BstSLI GKGCMC 1 cut(s) 322
BstUI CGCG 1 cut(s) 494
BstV1I GCAGC 3 cut(s) 707, 741, 797
BstX2I RGATCY 1 cut(s) 308
BstYI RGATCY 1 cut(s) 308
Bsu36I CCTNAGG 1 cut(s) 684
BsuRI GGCC 3 cut(s) 320, 471, 503
BtgZI GCGATG 1 cut(s) 994
BtrI CACGTC 1 cut(s) 1072
BtsIMutI CAGTG 1 cut(s) 11
BveI ACCTGC 1 cut(s) 754
Cac8I GCNNGC 4 cut(s) 356, 501, 513, 790
CaiI CAGNNNCTG 1 cut(s) 665
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 5 cut(s) 280, 318, 319, 469, 502
CseI GACGC 3 cut(s) 58, 125, 263
Csp6I GTAC 5 cut(s) 244, 388, 400, 961, 966
CviAII CATG 2 cut(s) 506, 835
CviQI GTAC 5 cut(s) 244, 388, 400, 961, 966
DdeI CTNAG 3 cut(s) 14, 23, 684
DpnI GATC 4 cut(s) 218, 310, 629, 681
DpnII GATC 4 cut(s) 216, 308, 627, 679
DraIII CACNNNGTG 1 cut(s) 424
Eam1104I CTCTTC 3 cut(s) 163, 377, 804
EarI CTCTTC 3 cut(s) 163, 377, 804
EciI GGCGGA 1 cut(s) 549
Eco130I CCWWGG 1 cut(s) 906
Eco24I GRGCYC 2 cut(s) 322, 673
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 2 cut(s) 401, 896
Eco81I CCTNAGG 1 cut(s) 684
Eco88I CYCGRG 1 cut(s) 408
EcoO109I RGGNCCY 1 cut(s) 318
EcoRII CCWGG 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 906
EcoT38I GRGCYC 2 cut(s) 322, 673
ErhI CCWWGG 1 cut(s) 906
FaeI CATG 2 cut(s) 509, 838
FaiI YATR 7 cut(s) 507, 586, 588, 719, 836, 1100, 1102
FaqI GGGAC 1 cut(s) 968
FatI CATG 2 cut(s) 505, 834
FauI CCCGC 2 cut(s) 321, 480
FbaI TGATCA 1 cut(s) 627
FblI GTMKAC 2 cut(s) 1033, 1074
Fnu4HI GCNGC 6 cut(s) 289, 472, 532, 696, 730, 786
FriOI GRGCYC 2 cut(s) 322, 673
Fsp4HI GCNGC 6 cut(s) 289, 472, 532, 696, 730, 786
GluI GCNGC 6 cut(s) 289, 472, 532, 696, 730, 786
GsaI CCCAGC 1 cut(s) 353
GsuI CTGGAG 3 cut(s) 687, 798, 804
HaeIII GGCC 3 cut(s) 320, 471, 503
HapII CCGG 3 cut(s) 106, 258, 475
HgaI GACGC 3 cut(s) 58, 125, 263
Hin1I GRCGYC 4 cut(s) 50, 117, 255, 441
Hin1II CATG 2 cut(s) 509, 838
HincII GTYRAC 2 cut(s) 903, 1075
HindII GTYRAC 2 cut(s) 903, 1075
HinfI GANTC 7 cut(s) 7, 18, 27, 166, 656, 702, 1001
HpaI GTTAAC 1 cut(s) 903
HpaII CCGG 3 cut(s) 106, 258, 475
HphI GGTGA 3 cut(s) 74, 211, 983
Hpy166II GTNNAC 6 cut(s) 283, 439, 903, 947, 1034, 1075
Hpy188I TCNGA 5 cut(s) 26, 216, 266, 369, 876
Hpy188III TCNNGA 7 cut(s) 408, 631, 660, 777, 925, 1109, 1126
Hpy8I GTNNAC 6 cut(s) 283, 439, 903, 947, 1034, 1075
Hpy99I CGWCG 4 cut(s) 52, 119, 257, 1076
HpyAV CCTTC 2 cut(s) 1015, 1057
HpyCH4III ACNGT 4 cut(s) 340, 543, 965, 1150
HpyCH4IV ACGT 5 cut(s) 390, 428, 441, 1071, 1160
HpyCH4V TGCA 4 cut(s) 92, 593, 785, 805
HpyF10VI GCNNNNNNNGC 3 cut(s) 355, 500, 746
HpyF3I CTNAG 3 cut(s) 14, 23, 684
HpySE526I ACGT 5 cut(s) 390, 428, 441, 1071, 1160
Hsp92I GRCGYC 4 cut(s) 50, 117, 255, 441
Hsp92II CATG 2 cut(s) 509, 838
Ksp22I TGATCA 1 cut(s) 627
KspAI GTTAAC 1 cut(s) 903
Kzo9I GATC 4 cut(s) 216, 308, 627, 679
LmnI GCTCC 3 cut(s) 496, 662, 668
Lsp1109I GCAGC 3 cut(s) 707, 741, 797
MaeII ACGT 5 cut(s) 390, 428, 441, 1071, 1160
MaeIII GTNAC 2 cut(s) 334, 424
MalI GATC 4 cut(s) 218, 310, 629, 681
MbiI CCGCTC 2 cut(s) 59, 278
MboI GATC 4 cut(s) 216, 308, 627, 679
MboII GAAGA 6 cut(s) 58, 150, 394, 646, 733, 821
MfeI CAATTG 1 cut(s) 825
MflI RGATCY 1 cut(s) 308
MhlI GDGCHC 3 cut(s) 322, 456, 673
MlyI GAGTC 1 cut(s) 21
MroXI GAANNNNTTC 2 cut(s) 860, 1046
MseI TTAA 5 cut(s) 639, 767, 902, 1116, 1152
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 3 cut(s) 106, 258, 475
MspR9I CCNGG 2 cut(s) 163, 476
MunI CAATTG 1 cut(s) 825
Mva1269I GAATGC 1 cut(s) 1139
MvaI CCWGG 1 cut(s) 163
MvnI CGCG 1 cut(s) 494
MwoI GCNNNNNNNGC 3 cut(s) 355, 500, 746
NciI CCSGG 1 cut(s) 476
NdeII GATC 4 cut(s) 216, 308, 627, 679
NlaIII CATG 2 cut(s) 509, 838
NlaIV GGNNCC 5 cut(s) 272, 319, 320, 670, 816
NmuCI GTSAC 2 cut(s) 334, 424
PctI GAATGC 1 cut(s) 1139
PdmI GAANNNNTTC 2 cut(s) 860, 1046
PfeI GAWTC 5 cut(s) 18, 166, 656, 702, 1001
PflFI GACNNNGTC 1 cut(s) 52
PflMI CCANNNNNTGG 1 cut(s) 511
PfoI TCCNGGA 1 cut(s) 161
PkrI GCNGC 6 cut(s) 290, 473, 533, 697, 731, 787
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
Ppu21I YACGTR 1 cut(s) 391
Psp6I CCWGG 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 349
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 5 cut(s) 272, 319, 320, 670, 816
PspOMI GGGCCC 1 cut(s) 318
PspPI GGNCC 5 cut(s) 280, 318, 319, 469, 502
PstNI CAGNNNCTG 1 cut(s) 665
PsuI RGATCY 1 cut(s) 308
PsyI GACNNNGTC 1 cut(s) 52
RsaI GTAC 5 cut(s) 245, 389, 401, 962, 967
RsaNI GTAC 5 cut(s) 244, 388, 400, 961, 966
SalI GTCGAC 1 cut(s) 1073
SaqAI TTAA 5 cut(s) 639, 767, 902, 1116, 1152
SatI GCNGC 6 cut(s) 289, 472, 532, 696, 730, 786
Sau3AI GATC 4 cut(s) 216, 308, 627, 679
Sau96I GGNCC 5 cut(s) 280, 318, 319, 469, 502
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 2 cut(s) 163, 476
SduI GDGCHC 3 cut(s) 322, 456, 673
SinI GGWCC 1 cut(s) 280
SmlI CTYRAG 1 cut(s) 1107
SmoI CTYRAG 1 cut(s) 1107
StyD4I CCNGG 2 cut(s) 161, 474
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 4 cut(s) 340, 543, 965, 1150
TaiI ACGT 5 cut(s) 393, 431, 444, 1074, 1163
TaqI TCGA 3 cut(s) 926, 999, 1074
TatI WGTACW 2 cut(s) 960, 965
TauI GCSGC 3 cut(s) 291, 474, 534
TfiI GAWTC 5 cut(s) 18, 166, 656, 702, 1001
Tru1I TTAA 5 cut(s) 639, 767, 902, 1116, 1152
Tru9I TTAA 5 cut(s) 639, 767, 902, 1116, 1152
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 2 cut(s) 334, 424
TseI GCWGC 3 cut(s) 695, 729, 785
Tsp45I GTSAC 2 cut(s) 334, 424
TspDTI ATGAA 2 cut(s) 734, 997
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 1 cut(s) 18
Tth111I GACNNNGTC 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 280
XapI RAATTY 3 cut(s) 77, 182, 603
XcmI CCANNNNNNNNNTGG 1 cut(s) 841
XmiI GTMKAC 2 cut(s) 1033, 1074
XmnI GAANNNNTTC 2 cut(s) 860, 1046
ZraI GACGTC 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.