MD00G1015700.v1.1

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003336
Physical Location & Seq
Reverse (-)
2247381 .. 2249286
1906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1015700.v1.1.491

Sequence Viewer

Length: 603 bp
ATGGCATTTTATGGACCGAGACTGGTTGTGCCTATAGACATGAAGAAGAAGCCATGGGAACAGAAGCTTCCTCTTCACAACCGATGGCACCCGAACATACCACCAGTGGCAGAAGTGGCAGCTGGAGAGGTTTTTCGGGTCGAGATGGTGGATTTCAGTGGGGGTGGTATTACCAAAGAGTACACTGCAGAGGACATCAAATATTTCGATCCCTCCATTGCTCATTACCTCAGTGACCCGATAAGAATTTTAGGTACGGATGGAATTCCAGCACAGCCAGGTGATCTTCTGGTAGTTGAGATATGCAACCTGGGTCCTCTCCCAGGAGATGAATGGGGTTTCACAGCAACATTTGACAGAGAAAATGGAGGGTGTTTCCTGACTGACCATTTTCCTTCTGCAACCAAAGCTATTTGGTATTTCAAAGGGATATACGCCTACTCGCCTCACATACCAGGAGTGAGATTCCCAGCGCTAATCCATCCTGGACTAGTTGGAACAGCACCGCCAATGGAACTCCTGAACATATGGAATGAAAGGCAGAGAGAACTTGAAGAAAATGGACTCAAGTCTATGAAATTATGTGAGGCTTTGCATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

201

Amino Acids

22.43

Weight (kDa)

5.47

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 12 - 187 2e-61 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 87
AciI CCGC 1 cut(s) 506
AclWI GGATC 1 cut(s) 203
AcsI RAATTY 2 cut(s) 246, 264
AfaI GTAC 2 cut(s) 182, 256
AfeI AGCGCT 1 cut(s) 474
AfiI CCNNNNNNNGG 1 cut(s) 323
AgsI TTSAA 2 cut(s) 424, 554
AhlI ACTAGT 1 cut(s) 490
AjnI CCWGG 5 cut(s) 277, 309, 322, 454, 484
AluBI AGCT 3 cut(s) 67, 122, 410
AluI AGCT 3 cut(s) 67, 122, 410
Alw26I GTCTC 1 cut(s) 13
AlwI GGATC 1 cut(s) 203
Aor51HI AGCGCT 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 2 cut(s) 246, 264
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 2 cut(s) 14, 314
AsuHPI GGTGA 1 cut(s) 293
AvaII GGWCC 2 cut(s) 14, 314
BanI GGYRCC 1 cut(s) 87
BbvI GCAGC 1 cut(s) 131
BccI CCATC 4 cut(s) 78, 139, 254, 489
BciT130I CCWGG 5 cut(s) 279, 311, 324, 456, 486
BcoDI GTCTC 1 cut(s) 13
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 2 cut(s) 33, 186
BfoI RGCGCY 1 cut(s) 476
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
Bme1390I CCNGG 5 cut(s) 279, 311, 324, 456, 486
Bme18I GGWCC 2 cut(s) 14, 314
BmgT120I GGNCC 2 cut(s) 14, 314
BmiI GGNNCC 2 cut(s) 89, 315
BmrFI CCNGG 5 cut(s) 279, 311, 324, 456, 486
BoxI GACNNNNGTC 1 cut(s) 568
BpmI CTGGAG 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 551
BsaJI CCNNGG 3 cut(s) 53, 310, 322
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 2 cut(s) 27, 104
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 5 cut(s) 279, 311, 324, 456, 486
BseDI CCNNGG 3 cut(s) 53, 310, 322
BseGI GGATG 2 cut(s) 265, 481
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 1 cut(s) 244
BseNI ACTGG 2 cut(s) 27, 104
BseXI GCAGC 1 cut(s) 131
BseYI CCCAGC 1 cut(s) 469
BshNI GGYRCC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 13
Bsp143I GATC 2 cut(s) 208, 283
Bsp19I CCATGG 1 cut(s) 53
BspACI CCGC 1 cut(s) 506
BspCNI CTCAG 1 cut(s) 243
BspLI GGNNCC 2 cut(s) 89, 315
BspMAI CTGCAG 1 cut(s) 190
BspPI GGATC 1 cut(s) 203
BspT107I GGYRCC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 2 cut(s) 27, 104
BssECI CCNNGG 3 cut(s) 53, 310, 322
BssMI GATC 2 cut(s) 208, 283
BssT1I CCWWGG 1 cut(s) 53
Bst2UI CCWGG 5 cut(s) 279, 311, 324, 456, 486
Bst6I CTCTTC 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 230
BstDSI CCRYGG 1 cut(s) 53
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 2 cut(s) 265, 481
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 2 cut(s) 211, 286
BstMAI GTCTC 1 cut(s) 13
BstMBI GATC 2 cut(s) 208, 283
BstMWI GCNNNNNNNGC 2 cut(s) 116, 407
BstNI CCWGG 5 cut(s) 279, 311, 324, 456, 486
BstPAI GACNNNNGTC 1 cut(s) 568
BstSCI CCNGG 5 cut(s) 277, 309, 322, 454, 484
BstSFI CTRYAG 2 cut(s) 33, 186
BstV1I GCAGC 1 cut(s) 131
BtgI CCRYGG 1 cut(s) 53
BtsCI GGATG 2 cut(s) 265, 481
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 4 cut(s) 111, 163, 183, 238
CfoI GCGC 1 cut(s) 475
Cfr13I GGNCC 2 cut(s) 14, 314
Csp6I GTAC 2 cut(s) 181, 255
CviAII CATG 2 cut(s) 40, 54
CviJI RGCY 6 cut(s) 52, 67, 122, 277, 410, 590
CviKI_1 RGCY 6 cut(s) 52, 67, 122, 277, 410, 590
CviQI GTAC 2 cut(s) 181, 255
DdeI CTNAG 1 cut(s) 230
DpnI GATC 2 cut(s) 210, 285
DpnII GATC 2 cut(s) 208, 283
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 53
Eco47I GGWCC 2 cut(s) 14, 314
Eco47III AGCGCT 1 cut(s) 474
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 264
EcoRII CCWGG 5 cut(s) 277, 309, 322, 454, 484
EcoT14I CCWWGG 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 2 cut(s) 43, 57
FatI CATG 2 cut(s) 39, 53
FauNDI CATATG 1 cut(s) 527
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 2 cut(s) 272, 468
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 1 cut(s) 491
GlaI GCGC 1 cut(s) 474
GluI GCNGC 1 cut(s) 120
GsaI CCCAGC 1 cut(s) 473
GsuI CTGGAG 1 cut(s) 144
HaeII RGCGCY 1 cut(s) 476
HhaI GCGC 1 cut(s) 475
Hin1II CATG 2 cut(s) 43, 57
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 2 cut(s) 465, 564
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 1 cut(s) 183
Hpy188III TCNNGA 3 cut(s) 142, 379, 520
Hpy8I GTNNAC 1 cut(s) 183
HpyAV CCTTC 1 cut(s) 405
HpyCH4V TGCA 4 cut(s) 188, 306, 401, 595
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 407
HpyF3I CTNAG 1 cut(s) 230
Hsp92II CATG 2 cut(s) 43, 57
HspAI GCGC 1 cut(s) 473
Kzo9I GATC 2 cut(s) 208, 283
Lsp1109I GCAGC 1 cut(s) 131
MaeI CTAG 1 cut(s) 491
MaeIII GTNAC 1 cut(s) 233
MalI GATC 2 cut(s) 210, 285
MboI GATC 2 cut(s) 208, 283
MboII GAAGA 5 cut(s) 55, 58, 65, 278, 566
MluCI AATT 3 cut(s) 246, 264, 578
MlyI GAGTC 1 cut(s) 558
MmeI TCCRAC 1 cut(s) 475
MnlI CCTC 9 cut(s) 81, 121, 184, 223, 239, 327, 362, 456, 580
MspA1I CMGCKG 1 cut(s) 122
MspR9I CCNGG 5 cut(s) 279, 311, 324, 456, 486
MvaI CCWGG 5 cut(s) 279, 311, 324, 456, 486
MwoI GCNNNNNNNGC 2 cut(s) 116, 407
NcoI CCATGG 1 cut(s) 53
NdeI CATATG 1 cut(s) 527
NdeII GATC 2 cut(s) 208, 283
NlaIII CATG 2 cut(s) 43, 57
NlaIV GGNNCC 2 cut(s) 89, 315
NmuCI GTSAC 1 cut(s) 233
PfeI GAWTC 1 cut(s) 465
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 1 cut(s) 121
PleI GAGTC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 558
PpuMI RGGWCCY 1 cut(s) 314
PshAI GACNNNNGTC 1 cut(s) 568
Psp5II RGGWCCY 1 cut(s) 314
Psp6I CCWGG 5 cut(s) 277, 309, 322, 454, 484
PspFI CCCAGC 1 cut(s) 469
PspGI CCWGG 5 cut(s) 277, 309, 322, 454, 484
PspN4I GGNNCC 2 cut(s) 89, 315
PspPI GGNCC 2 cut(s) 14, 314
PspPPI RGGWCCY 1 cut(s) 314
PstI CTGCAG 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 2 cut(s) 182, 256
RsaNI GTAC 2 cut(s) 181, 255
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 2 cut(s) 208, 283
Sau96I GGNCC 2 cut(s) 14, 314
SchI GAGTC 1 cut(s) 558
ScrFI CCNGG 5 cut(s) 279, 311, 324, 456, 486
SetI ASST 8 cut(s) 69, 124, 132, 231, 256, 283, 312, 412
SfcI CTRYAG 2 cut(s) 33, 186
SinI GGWCC 2 cut(s) 14, 314
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
SpeI ACTAGT 1 cut(s) 490
Sse9I AATT 3 cut(s) 246, 264, 578
SsiI CCGC 1 cut(s) 506
SspI AATATT 1 cut(s) 203
SspMI CTAG 1 cut(s) 491
StyD4I CCNGG 5 cut(s) 277, 309, 322, 454, 484
StyI CCWWGG 1 cut(s) 53
TaqI TCGA 2 cut(s) 141, 207
TaqII GACCGA 1 cut(s) 31
TasI AATT 3 cut(s) 246, 264, 578
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 1 cut(s) 465
TscAI CASTG 4 cut(s) 111, 163, 190, 238
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 1 cut(s) 119
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 4 cut(s) 56, 345, 549, 590
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 4 cut(s) 111, 163, 190, 238
VpaK11BI GGWCC 2 cut(s) 14, 314
XagI CCTNNNNNAGG 1 cut(s) 321
XapI RAATTY 2 cut(s) 246, 264
XcmI CCANNNNNNNNNTGG 1 cut(s) 330
XspI CTAG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.