pycom02g14860

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003336
Physical Location & Seq
Forward (+)
11522223 .. 11524124
1902 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g14860.2

Sequence Viewer

Length: 849 bp
ATGCCTACTCGCCTCAGCATGGCAGGAGTGAGATTCCCAGGGCTAACCCATCCTGGACTAGTTGGAACAGCACCGTCAATGGAACTCCTGAATATATGGAATGAAAGGGAGAGAGAACTTGAAGAAAATGGACTCAAGTCTATGAAATTATGTGAGGTTTTGCATCAACGACCATTGGCTAACCTACCATCAACAAAAGGTTGCGTCCTTGGAGGGATCAAAGAGGGCACTCCTGAATGGGAAAAGATAGCCATGGAGGCCGCAAGAACAATTCCAGGAAGAGAAAATGGAGGCAATTGTGACATAAAAAATCTTAGTAGTGGTTCAAAGATATACCTTCCAGTATTTGTTGAAGGAGCAAATCTCAGTACTGGTGACATGCACTTTTCCCAGGGTGATGGTGAAATCTCACTCTGCGGGGCAATTGAGATGAGCGGTTTCCTGGAGCTCAAGTGTGAAATTATAAGAGGTGGAATGAAAGAATACCTCACACCAATGGGGCCAACTCCGCTTCACGTGAACCCAATCTTTGAGATAGGCCCGATTGAGCCAAGATTCTCAGAATTGTTGGTGTTTGAGGGCATCAGTGTTGACGAGAGTGGGAAGCAGCATTACCTAGATGCAACTGTGGCTTACAAGCTAGCAGTACTCAATGCTATTGACTACCTCTCTAAATTTGGATACTCCAAAGAACAGAGCTATCTTCTGTTGTCACGCTGCCCGTGTGAGGGAAGAATTTCAGGAATAGTTGACTCTCCCAATGCTGTTGCAACCTTAGCAATCCCAACAGCTATCTTTGACCAGGATATTGCCCAAAAGAAAACAAGGTTCCTGTTGGTCCCCGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

283

Amino Acids

30.71

Weight (kDa)

5.38

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 464
AccBSI CCGCTC 1 cut(s) 435
AciI CCGC 4 cut(s) 261, 417, 435, 509
AclWI GGATC 1 cut(s) 224
AcsI RAATTY 2 cut(s) 674, 735
AcvI CACGTG 1 cut(s) 517
AfaI GTAC 2 cut(s) 370, 648
AfiI CCNNNNNNNGG 3 cut(s) 19, 727, 728
AgsI TTSAA 3 cut(s) 122, 327, 353
AhlI ACTAGT 1 cut(s) 58
AjnI CCWGG 6 cut(s) 37, 52, 274, 390, 441, 801
AluBI AGCT 4 cut(s) 448, 640, 699, 791
AluI AGCT 4 cut(s) 448, 640, 699, 791
Alw21I GWGCWC 1 cut(s) 450
AlwI GGATC 1 cut(s) 224
AoxI GGCC 3 cut(s) 258, 500, 538
ApeKI GCWGC 2 cut(s) 607, 717
ApoI RAATTY 2 cut(s) 674, 735
Asp700I GAANNNNTTC 1 cut(s) 736
AspS9I GGNCC 3 cut(s) 500, 539, 838
AsuC2I CCSGG 1 cut(s) 843
AsuHPI GGTGA 3 cut(s) 386, 407, 413
AsuNHI GCTAGC 1 cut(s) 640
AvaII GGWCC 1 cut(s) 838
BaeGI GKGCMC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 450
BarI GAAGNNNNNNTAC 2 cut(s) 596, 628
BbrPI CACGTG 1 cut(s) 517
Bbv12I GWGCWC 1 cut(s) 450
BbvCI CCTCAGC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 619, 704
BccI CCATC 3 cut(s) 57, 196, 392
BciT130I CCWGG 6 cut(s) 39, 54, 276, 392, 443, 803
BciVI GTATCC 1 cut(s) 674
BcnI CCSGG 1 cut(s) 843
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 3 cut(s) 59, 617, 641
BfuI GTATCC 1 cut(s) 674
BglI GCCNNNNNGGC 1 cut(s) 257
BisI GCNGC 3 cut(s) 261, 608, 718
BlsI GCNGC 3 cut(s) 262, 609, 719
BmcAI AGTACT 2 cut(s) 370, 648
Bme1390I CCNGG 7 cut(s) 39, 54, 276, 392, 443, 803, 843
Bme18I GGWCC 1 cut(s) 838
BmgT120I GGNCC 3 cut(s) 500, 539, 838
BmiI GGNNCC 3 cut(s) 501, 830, 840
BmrFI CCNGG 7 cut(s) 39, 54, 276, 392, 443, 803, 843
BmsI GCATC 3 cut(s) 172, 591, 610
BmtI GCTAGC 1 cut(s) 644
BoxI GACNNNNGTC 1 cut(s) 136
BplI GAGNNNNNCTC 2 cut(s) 348, 380
BpmI CTGGAG 1 cut(s) 464
Bpu10I CCTNAGC 2 cut(s) 14, 775
BpuEI CTTGAG 2 cut(s) 119, 434
BpuMI CCSGG 1 cut(s) 843
BsaAI YACGTR 1 cut(s) 517
BsaJI CCNNGG 7 cut(s) 37, 38, 208, 252, 390, 391, 841
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 727, 728
Bse1I ACTGG 2 cut(s) 341, 376
BseBI CCWGG 6 cut(s) 39, 54, 276, 392, 443, 803
BseDI CCNNGG 7 cut(s) 37, 38, 208, 252, 390, 391, 841
BseGI GGATG 1 cut(s) 49
BseLI CCNNNNNNNGG 3 cut(s) 19, 727, 728
BseMII CTCAG 3 cut(s) 28, 379, 573
BseNI ACTGG 2 cut(s) 341, 376
BseSI GKGCMC 1 cut(s) 230
BseXI GCAGC 2 cut(s) 619, 704
BshFI GGCC 3 cut(s) 260, 502, 540
BsiHKAI GWGCWC 1 cut(s) 450
BsiSI CCGG 1 cut(s) 843
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 3 cut(s) 19, 727, 728
BsmFI GGGAC 1 cut(s) 824
BsnI GGCC 3 cut(s) 260, 502, 540
Bsp1286I GDGCHC 2 cut(s) 230, 450
Bsp143I GATC 1 cut(s) 216
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 4 cut(s) 261, 417, 435, 509
BspANI GGCC 3 cut(s) 260, 502, 540
BspCNI CTCAG 3 cut(s) 27, 378, 572
BspLI GGNNCC 3 cut(s) 501, 830, 840
BspOI GCTAGC 1 cut(s) 644
BspPI GGATC 1 cut(s) 224
BsrBI CCGCTC 1 cut(s) 435
BsrI ACTGG 2 cut(s) 341, 376
BssECI CCNNGG 7 cut(s) 37, 38, 208, 252, 390, 391, 841
BssMI GATC 1 cut(s) 216
BssT1I CCWWGG 2 cut(s) 208, 252
Bst2UI CCWGG 6 cut(s) 39, 54, 276, 392, 443, 803
Bst4CI ACNGT 2 cut(s) 75, 628
Bst6I CTCTTC 1 cut(s) 274
BstBAI YACGTR 1 cut(s) 517
BstC8I GCNNGC 1 cut(s) 642
BstDEI CTNAG 5 cut(s) 14, 314, 365, 559, 775
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 49
BstKTI GATC 1 cut(s) 219
BstMBI GATC 1 cut(s) 216
BstMWI GCNNNNNNNGC 4 cut(s) 257, 508, 629, 776
BstNI CCWGG 6 cut(s) 39, 54, 276, 392, 443, 803
BstNSI RCATGY 1 cut(s) 382
BstPAI GACNNNNGTC 1 cut(s) 136
BstSCI CCNGG 7 cut(s) 37, 52, 274, 390, 441, 801, 841
BstSLI GKGCMC 1 cut(s) 230
BstV1I GCAGC 2 cut(s) 619, 704
BstXI CCANNNNNNTGG 1 cut(s) 398
BsuI GTATCC 1 cut(s) 674
BsuRI GGCC 3 cut(s) 260, 502, 540
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 592
Cac8I GCNNGC 1 cut(s) 642
Cfr13I GGNCC 3 cut(s) 500, 539, 838
CseI GACGC 1 cut(s) 193
Csp6I GTAC 2 cut(s) 369, 647
CviAII CATG 3 cut(s) 19, 253, 379
CviQI GTAC 2 cut(s) 369, 647
DdeI CTNAG 5 cut(s) 14, 314, 365, 559, 775
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
Eam1104I CTCTTC 1 cut(s) 274
EarI CTCTTC 1 cut(s) 274
Ecl136II GAGCTC 1 cut(s) 448
Eco130I CCWWGG 2 cut(s) 208, 252
Eco24I GRGCYC 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 838
Eco53kI GAGCTC 1 cut(s) 448
Eco72I CACGTG 1 cut(s) 517
EcoICRI GAGCTC 1 cut(s) 448
EcoRII CCWGG 6 cut(s) 37, 52, 274, 390, 441, 801
EcoT14I CCWWGG 2 cut(s) 208, 252
EcoT38I GRGCYC 1 cut(s) 450
ErhI CCWWGG 2 cut(s) 208, 252
FaeI CATG 3 cut(s) 22, 256, 382
FaqI GGGAC 1 cut(s) 824
FatI CATG 3 cut(s) 18, 252, 378
FauI CCCGC 1 cut(s) 410
Fnu4HI GCNGC 3 cut(s) 261, 608, 718
FokI GGATG 1 cut(s) 36
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 3 cut(s) 261, 608, 718
FspBI CTAG 3 cut(s) 59, 617, 641
GluI GCNGC 3 cut(s) 261, 608, 718
GsuI CTGGAG 1 cut(s) 464
HaeIII GGCC 3 cut(s) 260, 502, 540
HapII CCGG 1 cut(s) 843
HgaI GACGC 1 cut(s) 193
Hin1II CATG 3 cut(s) 22, 256, 382
HincII GTYRAC 2 cut(s) 592, 751
HindII GTYRAC 2 cut(s) 592, 751
HinfI GANTC 4 cut(s) 33, 132, 555, 752
HpaII CCGG 1 cut(s) 843
HphI GGTGA 3 cut(s) 386, 407, 413
Hpy166II GTNNAC 3 cut(s) 520, 592, 751
Hpy188I TCNGA 1 cut(s) 562
Hpy188III TCNNGA 3 cut(s) 88, 233, 741
Hpy8I GTNNAC 3 cut(s) 520, 592, 751
HpyAV CCTTC 2 cut(s) 347, 347
HpyCH4III ACNGT 2 cut(s) 75, 628
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 4 cut(s) 163, 382, 623, 770
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 508, 629, 776
HpyF3I CTNAG 5 cut(s) 14, 314, 365, 559, 775
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 3 cut(s) 22, 256, 382
Kzo9I GATC 1 cut(s) 216
LmnI GCTCC 2 cut(s) 356, 445
Lsp1109I GCAGC 2 cut(s) 619, 704
LweI GCATC 3 cut(s) 172, 591, 610
MaeI CTAG 3 cut(s) 59, 617, 641
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 3 cut(s) 299, 374, 711
MalI GATC 1 cut(s) 218
MbiI CCGCTC 1 cut(s) 435
MboI GATC 1 cut(s) 216
MboII GAAGA 4 cut(s) 134, 291, 695, 744
MfeI CAATTG 2 cut(s) 295, 423
MhlI GDGCHC 2 cut(s) 230, 450
MluCI AATT 8 cut(s) 146, 270, 295, 423, 459, 563, 674, 735
MlyI GAGTC 2 cut(s) 126, 746
MmeI TCCRAC 1 cut(s) 43
MroXI GAANNNNTTC 1 cut(s) 736
MslI CAYNNNNRTG 1 cut(s) 494
MspI CCGG 1 cut(s) 843
MspR9I CCNGG 7 cut(s) 39, 54, 276, 392, 443, 803, 843
MunI CAATTG 2 cut(s) 295, 423
MvaI CCWGG 6 cut(s) 39, 54, 276, 392, 443, 803
MwoI GCNNNNNNNGC 4 cut(s) 257, 508, 629, 776
NciI CCSGG 1 cut(s) 843
NcoI CCATGG 1 cut(s) 252
NdeII GATC 1 cut(s) 216
NheI GCTAGC 1 cut(s) 640
NlaIII CATG 3 cut(s) 22, 256, 382
NlaIV GGNNCC 3 cut(s) 501, 830, 840
NmuCI GTSAC 3 cut(s) 299, 374, 711
NspI RCATGY 1 cut(s) 382
PasI CCCWGGG 2 cut(s) 38, 391
PdmI GAANNNNTTC 1 cut(s) 736
PfeI GAWTC 2 cut(s) 33, 555
PfoI TCCNGGA 3 cut(s) 52, 274, 441
PkrI GCNGC 3 cut(s) 262, 609, 719
PleI GAGTC 2 cut(s) 126, 746
PmaCI CACGTG 1 cut(s) 517
PmlI CACGTG 1 cut(s) 517
PpsI GAGTC 2 cut(s) 126, 746
Ppu21I YACGTR 1 cut(s) 517
PshAI GACNNNNGTC 1 cut(s) 136
PsiI TTATAA 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 450
Psp6I CCWGG 6 cut(s) 37, 52, 274, 390, 441, 801
PspCI CACGTG 1 cut(s) 517
PspGI CCWGG 6 cut(s) 37, 52, 274, 390, 441, 801
PspN4I GGNNCC 3 cut(s) 501, 830, 840
PspPI GGNCC 3 cut(s) 500, 539, 838
RsaI GTAC 2 cut(s) 370, 648
RsaNI GTAC 2 cut(s) 369, 647
RseI CAYNNNNRTG 1 cut(s) 494
SacI GAGCTC 1 cut(s) 450
SatI GCNGC 3 cut(s) 261, 608, 718
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 3 cut(s) 500, 539, 838
ScaI AGTACT 2 cut(s) 370, 648
SchI GAGTC 2 cut(s) 126, 746
ScrFI CCNGG 7 cut(s) 39, 54, 276, 392, 443, 803, 843
SduI GDGCHC 2 cut(s) 230, 450
SfaNI GCATC 3 cut(s) 172, 591, 610
SinI GGWCC 1 cut(s) 838
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 2 cut(s) 134, 449
SmoI CTYRAG 2 cut(s) 134, 449
SpeI ACTAGT 1 cut(s) 58
Sse9I AATT 8 cut(s) 146, 270, 295, 423, 459, 563, 674, 735
SsiI CCGC 4 cut(s) 261, 417, 435, 509
SspMI CTAG 3 cut(s) 59, 617, 641
SstI GAGCTC 1 cut(s) 450
StyD4I CCNGG 7 cut(s) 37, 52, 274, 390, 441, 801, 841
StyI CCWWGG 2 cut(s) 208, 252
TaaI ACNGT 2 cut(s) 75, 628
TaiI ACGT 1 cut(s) 519
TasI AATT 8 cut(s) 146, 270, 295, 423, 459, 563, 674, 735
TatI WGTACW 2 cut(s) 368, 646
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 2 cut(s) 33, 555
TscAI CASTG 1 cut(s) 592
TseFI GTSAC 3 cut(s) 299, 374, 711
TseI GCWGC 2 cut(s) 607, 717
Tsp45I GTSAC 3 cut(s) 299, 374, 711
TspDTI ATGAA 3 cut(s) 117, 158, 491
TspRI CASTG 1 cut(s) 592
VpaK11BI GGWCC 1 cut(s) 838
XapI RAATTY 2 cut(s) 674, 735
XceI RCATGY 1 cut(s) 382
XmnI GAANNNNTTC 1 cut(s) 736
XspI CTAG 3 cut(s) 59, 617, 641
ZrmI AGTACT 2 cut(s) 370, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.