Rw2G015450

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003336
Physical Location & Seq
Forward (+)
17895537 .. 17899076
3540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G015450.1

Sequence Viewer

Length: 1389 bp
ATGCTTCCTGATTCTCACCCTAATTCATCCATGGCTCCTCGGACTCCAAGATTAGTAGTTCCTATTGACTTAAGCAGCAAACCATGGCAGCAAAAGCTACCTCTTCACAACCGTTGGCACCCAGACATACCCCCGGTGGCTGAGGTCAAAACCGGTGAGTTATTTAGAGTAGAGATGGTAGACTGGACTGGAGGTGCTATTAATGATGATGACTCTGCAGTAGACATAAAAACCATAGACCTCTCAACTGTTCATTATCTCAGCGGACCGATTAGGATTTCAGACAAGGATGGAATTCCAGCACAGCCAGGTGATCTTCTGGTAGTTGAGATATGCAACTTGGGTCCTCTTCCAGGAGATGAATGGGGTTTCACAGCAACATTCGACAGAGAAAATGGAGGGGGATTTCTGACTGACCATTTTCCTTGTGCAACCAAAGCTATTTGGTATTTTGAAGGGATATATGCCTACTCTCCTCAAATACCAGGAGTGAGATTCCCAGGGTTAACACACCCTGGAATAGTTGGAACAGCACCATCAACGGAACTCTTGAACATATGGAATAAGAGGGAGAGAGAACTTGAGGAAAATGGAGTCAAGTCTCTGAAACTATGTGAGGTTGTGCACCAACGGCCCTTGGCTAGCCTACCATCAACAAAAGGTTGCGTCCTTGGAGGGATCAAAGAGGGCACTCCCGAATGGGAAAAGATAGCAGTGGAGGCCGCAAGAACAATTCCAGGAAGAGAAAATGGTGGCAATTGTGACATCAAGAATCTTAGTAGAGGTTCAAAGGTATACCTTCCAGTATTCATTGAAGGAGCAAATCTTAGCACTGGTGACATGCACTTTTCACAGGGAGATGGTGAAATCGCATTCTGTGGTGCGATTGAGATGAGTGGTTTCCTGGAGCTCAAGTGTGAAATTATAAGGGATGGAATGAAAGAATACCTAACACCGATGGGGCCAACTCCTCTTCATGTGAATCCAATCTTTGAAATAGGTCCCGTCGAGCCAAGATTCTCAGAATGGTTGGTATTTGAGGGCATTAGTGTTGATGAGAGTGGGAAGCAGCACTACCTAGATGCAACTGTTGCTTACAAGCGTGCAGTACTGAATGCCATCGACTACCTCTCTAAATTTGGATACTCAAAAGAACAGAGCTACCTTCTGCTGTCATGCTGTCCATGCGAGGGAAGAATTTCTGGAATAGTCGACGCTCCCAATGCTGTTGCAACCCTAGCAATCCCAACAGCTATCTTTGACCAGGATATTCGACCAAAAACGAACAAGGTCCCGGTTGGTCCCCGGATAGTGAGGACACCAGATGTCCTAAAATGTACTTACGATGGAAATTTGCCAACGACAAGGAACCCTAGCTCAGCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

462

Amino Acids

50.56

Weight (kDa)

5.27

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 22 - 422 4.6e-171 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 926
AccB1I GGYRCC 1 cut(s) 117
AccI GTMKAC 4 cut(s) 180, 222, 795, 1212
AciI CCGC 2 cut(s) 264, 723
AclWI GGATC 1 cut(s) 686
AcsI RAATTY 4 cut(s) 294, 1136, 1197, 1351
AfaI GTAC 2 cut(s) 1110, 1339
AfiI CCNNNNNNNGG 2 cut(s) 353, 1190
AflII CTTAAG 1 cut(s) 70
AgeI ACCGGT 1 cut(s) 152
AgsI TTSAA 5 cut(s) 455, 553, 789, 815, 995
AjnI CCWGG 8 cut(s) 307, 352, 484, 499, 514, 736, 903, 1263
AluBI AGCT 6 cut(s) 97, 440, 910, 1161, 1253, 1377
AluI AGCT 6 cut(s) 97, 440, 910, 1161, 1253, 1377
Alw21I GWGCWC 2 cut(s) 627, 912
Alw26I GTCTC 1 cut(s) 606
Alw44I GTGCAC 1 cut(s) 623
AlwI GGATC 1 cut(s) 686
AoxI GGCC 3 cut(s) 632, 720, 962
ApaLI GTGCAC 1 cut(s) 623
ApeKI GCWGC 3 cut(s) 75, 88, 1069
ApoI RAATTY 4 cut(s) 294, 1136, 1197, 1351
AseI ATTAAT 1 cut(s) 201
AsiGI ACCGGT 1 cut(s) 152
Asp700I GAANNNNTTC 1 cut(s) 1198
AspS9I GGNCC 7 cut(s) 266, 344, 633, 962, 1001, 1291, 1301
AsuC2I CCSGG 3 cut(s) 134, 1295, 1306
AsuHPI GGTGA 5 cut(s) 8, 167, 323, 848, 875
AsuNHI GCTAGC 1 cut(s) 641
AvaII GGWCC 5 cut(s) 266, 344, 1001, 1291, 1301
BaeGI GKGCMC 2 cut(s) 627, 692
BanI GGYRCC 1 cut(s) 117
BanII GRGCYC 1 cut(s) 912
BarI GAAGNNNNNNTAC 2 cut(s) 1058, 1090
Bbv12I GWGCWC 2 cut(s) 627, 912
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 87, 100, 1081
BccI CCATC 9 cut(s) 169, 284, 544, 658, 854, 926, 952, 1127, 1340
BceAI ACGGC 1 cut(s) 647
BciT130I CCWGG 8 cut(s) 309, 354, 486, 501, 516, 738, 905, 1265
BciVI GTATCC 1 cut(s) 1136
BcnI CCSGG 3 cut(s) 134, 1295, 1306
BcoDI GTCTC 1 cut(s) 606
BfaI CTAG 4 cut(s) 642, 1079, 1238, 1374
BfmI CTRYAG 1 cut(s) 216
BfrI CTTAAG 1 cut(s) 70
BfuI GTATCC 1 cut(s) 1136
BisI GCNGC 4 cut(s) 76, 89, 723, 1070
BlpI GCTNAGC 1 cut(s) 1378
BlsI GCNGC 4 cut(s) 77, 90, 724, 1071
BmcAI AGTACT 1 cut(s) 1110
Bme18I GGWCC 5 cut(s) 266, 344, 1001, 1291, 1301
BmgT120I GGNCC 7 cut(s) 266, 344, 633, 962, 1001, 1291, 1301
BmiI GGNNCC 8 cut(s) 36, 119, 345, 963, 1003, 1293, 1303, 1370
BmsI GCATC 1 cut(s) 1072
BmtI GCTAGC 1 cut(s) 645
BpmI CTGGAG 2 cut(s) 210, 926
Bpu10I CCTNAGC 1 cut(s) 141
Bpu1102I GCTNAGC 1 cut(s) 1378
BpuEI CTTGAG 2 cut(s) 602, 896
BpuMI CCSGG 3 cut(s) 134, 1295, 1306
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 899, 929
Bsc4I CCNNNNNNNGG 2 cut(s) 353, 1190
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 4 cut(s) 188, 193, 803, 838
BseBI CCWGG 8 cut(s) 309, 354, 486, 501, 516, 738, 905, 1265
BseGI GGATG 3 cut(s) 26, 295, 937
BseLI CCNNNNNNNGG 2 cut(s) 353, 1190
BseMII CTCAG 3 cut(s) 132, 274, 1035
BseNI ACTGG 4 cut(s) 188, 193, 803, 838
BseRI GAGGAG 3 cut(s) 27, 465, 960
BseSI GKGCMC 2 cut(s) 627, 692
BseXI GCAGC 3 cut(s) 87, 100, 1081
BsgI GTGCAG 1 cut(s) 1125
BshFI GGCC 3 cut(s) 634, 722, 964
BshNI GGYRCC 1 cut(s) 117
BshTI ACCGGT 1 cut(s) 152
BsiHKAI GWGCWC 2 cut(s) 627, 912
BsiSI CCGG 4 cut(s) 134, 153, 1295, 1306
BslFI GGGAC 3 cut(s) 987, 1277, 1287
BslI CCNNNNNNNGG 2 cut(s) 353, 1190
BsmAI GTCTC 1 cut(s) 606
BsmFI GGGAC 3 cut(s) 987, 1277, 1287
BsmI GAATGC 2 cut(s) 872, 1120
BsnI GGCC 3 cut(s) 634, 722, 964
Bsp1286I GDGCHC 3 cut(s) 627, 692, 912
Bsp143I GATC 2 cut(s) 313, 678
Bsp1720I GCTNAGC 1 cut(s) 1378
Bsp19I CCATGG 2 cut(s) 30, 83
BspACI CCGC 2 cut(s) 264, 723
BspANI GGCC 3 cut(s) 634, 722, 964
BspCNI CTCAG 3 cut(s) 133, 273, 1034
BspLI GGNNCC 8 cut(s) 36, 119, 345, 963, 1003, 1293, 1303, 1370
BspMAI CTGCAG 1 cut(s) 220
BspOI GCTAGC 1 cut(s) 645
BspPI GGATC 1 cut(s) 686
BspT107I GGYRCC 1 cut(s) 117
BspTI CTTAAG 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 4 cut(s) 188, 193, 803, 838
BssAI RCCGGY 1 cut(s) 152
BssMI GATC 2 cut(s) 313, 678
BssNAI GTATAC 1 cut(s) 796
BssT1I CCWWGG 4 cut(s) 30, 83, 636, 670
Bst1107I GTATAC 1 cut(s) 796
Bst2UI CCWGG 8 cut(s) 309, 354, 486, 501, 516, 738, 905, 1265
Bst4CI ACNGT 3 cut(s) 113, 250, 1090
Bst6I CTCTTC 4 cut(s) 108, 354, 736, 978
BstAFI CTTAAG 1 cut(s) 70
BstAPI GCANNNNNTGC 1 cut(s) 1091
BstC8I GCNNGC 2 cut(s) 643, 1104
BstDEI CTNAG 6 cut(s) 141, 260, 776, 827, 1021, 1378
BstDSI CCRYGG 2 cut(s) 30, 83
BstENI CCTNNNNNAGG 1 cut(s) 351
BstF5I GGATG 3 cut(s) 26, 295, 937
BstKTI GATC 2 cut(s) 316, 681
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 2 cut(s) 313, 678
BstMWI GCNNNNNNNGC 8 cut(s) 94, 437, 631, 719, 1091, 1185, 1223, 1238
BstNI CCWGG 8 cut(s) 309, 354, 486, 501, 516, 738, 905, 1265
BstNSI RCATGY 1 cut(s) 844
BstSFI CTRYAG 1 cut(s) 216
BstSLI GKGCMC 2 cut(s) 627, 692
BstV1I GCAGC 3 cut(s) 87, 100, 1081
BstZ17I GTATAC 1 cut(s) 796
BsuI GTATCC 1 cut(s) 1136
BsuRI GGCC 3 cut(s) 634, 722, 964
BtgI CCRYGG 2 cut(s) 30, 83
BtsCI GGATG 3 cut(s) 26, 295, 937
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 2 cut(s) 720, 831
Cac8I GCNNGC 2 cut(s) 643, 1104
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 7 cut(s) 266, 344, 633, 962, 1001, 1291, 1301
CpoI CGGWCCG 1 cut(s) 266
CseI GACGC 2 cut(s) 655, 1223
Csp6I GTAC 2 cut(s) 1109, 1338
CspAI ACCGGT 1 cut(s) 152
CspI CGGWCCG 1 cut(s) 266
CviAII CATG 6 cut(s) 31, 84, 841, 977, 1176, 1185
CviQI GTAC 2 cut(s) 1109, 1338
DdeI CTNAG 6 cut(s) 141, 260, 776, 827, 1021, 1378
DpnI GATC 2 cut(s) 315, 680
DpnII GATC 2 cut(s) 313, 678
Eam1104I CTCTTC 4 cut(s) 108, 354, 736, 978
EarI CTCTTC 4 cut(s) 108, 354, 736, 978
Ecl136II GAGCTC 1 cut(s) 910
Eco130I CCWWGG 4 cut(s) 30, 83, 636, 670
Eco24I GRGCYC 1 cut(s) 912
Eco47I GGWCC 5 cut(s) 266, 344, 1001, 1291, 1301
Eco53kI GAGCTC 1 cut(s) 910
EcoICRI GAGCTC 1 cut(s) 910
EcoNI CCTNNNNNAGG 1 cut(s) 351
EcoO109I RGGNCCY 3 cut(s) 344, 1001, 1291
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 8 cut(s) 307, 352, 484, 499, 514, 736, 903, 1263
EcoT14I CCWWGG 4 cut(s) 30, 83, 636, 670
EcoT38I GRGCYC 1 cut(s) 912
ErhI CCWWGG 4 cut(s) 30, 83, 636, 670
FaeI CATG 6 cut(s) 34, 87, 844, 980, 1179, 1188
FaqI GGGAC 3 cut(s) 987, 1277, 1287
FatI CATG 6 cut(s) 30, 83, 840, 976, 1175, 1184
FauNDI CATATG 1 cut(s) 557
FblI GTMKAC 4 cut(s) 180, 222, 795, 1212
Fnu4HI GCNGC 4 cut(s) 76, 89, 723, 1070
FokI GGATG 3 cut(s) 13, 302, 944
FriOI GRGCYC 1 cut(s) 912
Fsp4HI GCNGC 4 cut(s) 76, 89, 723, 1070
FspBI CTAG 4 cut(s) 642, 1079, 1238, 1374
GluI GCNGC 4 cut(s) 76, 89, 723, 1070
GsuI CTGGAG 2 cut(s) 210, 926
HaeIII GGCC 3 cut(s) 634, 722, 964
HapII CCGG 4 cut(s) 134, 153, 1295, 1306
HgaI GACGC 2 cut(s) 655, 1223
Hin1II CATG 6 cut(s) 34, 87, 844, 980, 1179, 1188
HincII GTYRAC 2 cut(s) 507, 1213
HindII GTYRAC 2 cut(s) 507, 1213
HinfI GANTC 8 cut(s) 11, 43, 212, 495, 594, 772, 982, 1017
HpaI GTTAAC 1 cut(s) 507
HpaII CCGG 4 cut(s) 134, 153, 1295, 1306
HphI GGTGA 5 cut(s) 8, 167, 323, 848, 875
Hpy166II GTNNAC 6 cut(s) 181, 223, 507, 625, 796, 1213
Hpy188I TCNGA 5 cut(s) 42, 283, 411, 606, 1024
Hpy188III TCNNGA 5 cut(s) 8, 550, 695, 769, 1203
Hpy8I GTNNAC 6 cut(s) 181, 223, 507, 625, 796, 1213
Hpy99I CGWCG 2 cut(s) 1010, 1217
HpyAV CCTTC 4 cut(s) 449, 809, 809, 1175
HpyCH4III ACNGT 3 cut(s) 113, 250, 1090
HpyCH4V TGCA 8 cut(s) 218, 336, 431, 625, 844, 1085, 1106, 1232
HpyF10VI GCNNNNNNNGC 8 cut(s) 94, 437, 631, 719, 1091, 1185, 1223, 1238
HpyF3I CTNAG 6 cut(s) 141, 260, 776, 827, 1021, 1378
Hsp92II CATG 6 cut(s) 34, 87, 844, 980, 1179, 1188
KspAI GTTAAC 1 cut(s) 507
Kzo9I GATC 2 cut(s) 313, 678
LmnI GCTCC 4 cut(s) 40, 818, 907, 1222
Lsp1109I GCAGC 3 cut(s) 87, 100, 1081
LweI GCATC 1 cut(s) 1072
MaeI CTAG 4 cut(s) 642, 1079, 1238, 1374
MaeIII GTNAC 2 cut(s) 761, 836
MalI GATC 2 cut(s) 315, 680
MboI GATC 2 cut(s) 313, 678
MboII GAAGA 6 cut(s) 95, 308, 341, 753, 965, 1206
MfeI CAATTG 1 cut(s) 757
MhlI GDGCHC 3 cut(s) 627, 692, 912
MluCI AATT 8 cut(s) 22, 294, 732, 757, 921, 1136, 1197, 1351
MlyI GAGTC 3 cut(s) 37, 206, 603
MmeI TCCRAC 1 cut(s) 505
MroXI GAANNNNTTC 1 cut(s) 1198
MseI TTAA 3 cut(s) 71, 201, 506
MspA1I CMGCKG 1 cut(s) 264
MspCI CTTAAG 1 cut(s) 70
MspI CCGG 4 cut(s) 134, 153, 1295, 1306
MunI CAATTG 1 cut(s) 757
Mva1269I GAATGC 2 cut(s) 872, 1120
MvaI CCWGG 8 cut(s) 309, 354, 486, 501, 516, 738, 905, 1265
MwoI GCNNNNNNNGC 8 cut(s) 94, 437, 631, 719, 1091, 1185, 1223, 1238
NciI CCSGG 3 cut(s) 134, 1295, 1306
NcoI CCATGG 2 cut(s) 30, 83
NdeI CATATG 1 cut(s) 557
NdeII GATC 2 cut(s) 313, 678
NheI GCTAGC 1 cut(s) 641
NlaIII CATG 6 cut(s) 34, 87, 844, 980, 1179, 1188
NlaIV GGNNCC 8 cut(s) 36, 119, 345, 963, 1003, 1293, 1303, 1370
NmuCI GTSAC 2 cut(s) 761, 836
NspI RCATGY 1 cut(s) 844
PasI CCCWGGG 1 cut(s) 500
PctI GAATGC 2 cut(s) 872, 1120
PdmI GAANNNNTTC 1 cut(s) 1198
PfeI GAWTC 5 cut(s) 11, 495, 772, 982, 1017
PfoI TCCNGGA 3 cut(s) 352, 736, 903
PinAI ACCGGT 1 cut(s) 152
PkrI GCNGC 4 cut(s) 77, 90, 724, 1071
PleI GAGTC 3 cut(s) 37, 206, 602
PpsI GAGTC 3 cut(s) 37, 206, 602
PpuMI RGGWCCY 3 cut(s) 344, 1001, 1291
PshBI ATTAAT 1 cut(s) 201
PsiI TTATAA 1 cut(s) 926
Psp124BI GAGCTC 1 cut(s) 912
Psp5II RGGWCCY 3 cut(s) 344, 1001, 1291
Psp6I CCWGG 8 cut(s) 307, 352, 484, 499, 514, 736, 903, 1263
PspGI CCWGG 8 cut(s) 307, 352, 484, 499, 514, 736, 903, 1263
PspN4I GGNNCC 8 cut(s) 36, 119, 345, 963, 1003, 1293, 1303, 1370
PspPI GGNCC 7 cut(s) 266, 344, 633, 962, 1001, 1291, 1301
PspPPI RGGWCCY 3 cut(s) 344, 1001, 1291
PstI CTGCAG 1 cut(s) 220
RsaI GTAC 2 cut(s) 1110, 1339
RsaNI GTAC 2 cut(s) 1109, 1338
Rsr2I CGGWCCG 1 cut(s) 266
RsrII CGGWCCG 1 cut(s) 266
SacI GAGCTC 1 cut(s) 912
SalI GTCGAC 1 cut(s) 1211
SaqAI TTAA 3 cut(s) 71, 201, 506
SatI GCNGC 4 cut(s) 76, 89, 723, 1070
Sau3AI GATC 2 cut(s) 313, 678
Sau96I GGNCC 7 cut(s) 266, 344, 633, 962, 1001, 1291, 1301
ScaI AGTACT 1 cut(s) 1110
SchI GAGTC 3 cut(s) 37, 206, 603
SduI GDGCHC 3 cut(s) 627, 692, 912
SfaNI GCATC 1 cut(s) 1072
SfcI CTRYAG 1 cut(s) 216
SinI GGWCC 5 cut(s) 266, 344, 1001, 1291, 1301
SmlI CTYRAG 3 cut(s) 70, 581, 911
SmoI CTYRAG 3 cut(s) 70, 581, 911
Sse9I AATT 8 cut(s) 22, 294, 732, 757, 921, 1136, 1197, 1351
SsiI CCGC 2 cut(s) 264, 723
SspMI CTAG 4 cut(s) 642, 1079, 1238, 1374
SstI GAGCTC 1 cut(s) 912
StyI CCWWGG 4 cut(s) 30, 83, 636, 670
TaaI ACNGT 3 cut(s) 113, 250, 1090
TaqI TCGA 5 cut(s) 384, 1008, 1122, 1212, 1273
TaqII GACCGA 1 cut(s) 283
TasI AATT 8 cut(s) 22, 294, 732, 757, 921, 1136, 1197, 1351
TatI WGTACW 2 cut(s) 1108, 1337
TauI GCSGC 1 cut(s) 725
TfiI GAWTC 5 cut(s) 11, 495, 772, 982, 1017
Tru1I TTAA 3 cut(s) 71, 201, 506
Tru9I TTAA 3 cut(s) 71, 201, 506
TscAI CASTG 2 cut(s) 720, 838
TseFI GTSAC 2 cut(s) 761, 836
TseI GCWGC 3 cut(s) 75, 88, 1069
Tsp45I GTSAC 2 cut(s) 761, 836
TspDTI ATGAA 6 cut(s) 15, 242, 375, 799, 953, 965
TspGWI ACGGA 1 cut(s) 557
TspRI CASTG 2 cut(s) 720, 838
Vha464I CTTAAG 1 cut(s) 70
VneI GTGCAC 1 cut(s) 623
VpaK11BI GGWCC 5 cut(s) 266, 344, 1001, 1291, 1301
VspI ATTAAT 1 cut(s) 201
XagI CCTNNNNNAGG 1 cut(s) 351
XapI RAATTY 4 cut(s) 294, 1136, 1197, 1351
XceI RCATGY 1 cut(s) 844
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XmiI GTMKAC 4 cut(s) 180, 222, 795, 1212
XmnI GAANNNNTTC 1 cut(s) 1198
XspI CTAG 4 cut(s) 642, 1079, 1238, 1374
ZrmI AGTACT 1 cut(s) 1110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.