Rroxscaffold_2G00136180

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003336
Physical Location & Seq
Reverse (-)
73833531 .. 73837549
4019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136180.1

Sequence Viewer

Length: 1359 bp
ATGGCTCCTCGGACTCCAAGATTAGTAGTTCCTATTGACTTAAGCAGCAAACCATGGCAGCAAAAGCTACCTCTTCACAACCGTTGGCACCCAGACATACCCCCGGTGGCTGAGGTCAAGACCGGTGAGTTATTTAGAGTAGAGATGGTAGATTGGACTGGAGGTGCTATTAACGATGATGACTCTGCAGTAGACGTAAAAAACATAGAACTCTCAACTGTTCATTATCTCAGTGGACCGATTAGGATTTCAGACAAGGATGGAATTCCAGCACAGCCAGGTGATCTTCTGGTAGTTGAGATATGCAACTTGGGTCCTCTTCCAGGAGATGAATGGGGTTTCACAGCAACATTTGACAGAGAAAATGGAGGGGGATTTCTGACTGACCATTTTCCTGGTGCAACCAAAGCTATTTGGTATTTTGAAGGGATATATGCCTACTCTCCTCAAATACCAGGAGTGAGATTCCCAGGGTTAACACACCCTGGAATAGTTGGAACAGCACCATCGACGGAACTCTTGAACATATGGAATAAGAGAGAGAGAGAACTTGAGGAAAATGGAGTCAAGTCTCTGAAACTATGTGAGGTTGTGCACCAACGGCCCTTGGCTAGCCTACCATCAACAAAAGGTTGCGTCCTTGGAGGGATCAAAGAGGGCACTCCCGAATGGGAAAAGATAGCAGTGGAGGCTGCAAGAACAATTCCAGGAAGAGAAAATGGTGGCAATTGTGACATCAAGAATCTTAGTAGAGGTTCAAAGGTATACCTTCCAGTATTCATTGAAGGAGCAAATCTTAGCACTGGTGACATGCACTTTTCACAGGGAGATGGTGAAATTGCATTCTGTGGTGCGATTGAGATGAGTGGTTTCCTGGAGCTCAAGTGTGAAATTATAAGGGATGGAATGAAAGAATACCTTACACCGATGGGGCCAACTCCTCTTCATGTGAATCCAATCTTTGAAATAGGTCCCGTCGAGCCAAGATTCTCGGAATGGTTGGTATTTGAGGGCATCAGTGTTGATGAGAGTGGGAAGCAGCACTACCTAGATGCAACTGTTGCTTACAAGCGTGCAGTACTGAATGCCATCGACTACCTCTCTAAATTTGGATACTCAAAAGAACAGAGCTACCTTCTGCTGTCATGCTGTCCATGCGAGGGAAGAATTTCTGGAATAGTCGACGCTCCCAATGCTCTTGCAACCCTAGCAATCCCAACAGCTATCTTTGACCAGGATATTCGACCAAAAACGAACAAGGTCCCGGTTGGTCCCCGGATAATGAGGACACCAGATGTCCTAAAATGTACTTACGATGGAAATTTGCCAACGACAAGGAACCCTAGCTCAGCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

452

Amino Acids

49.51

Weight (kDa)

5.28

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 12 - 412 1.8e-170 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 896
AccB1I GGYRCC 1 cut(s) 87
AccI GTMKAC 3 cut(s) 192, 765, 1182
AclWI GGATC 1 cut(s) 656
AcsI RAATTY 4 cut(s) 264, 1106, 1167, 1321
AfaI GTAC 2 cut(s) 1080, 1309
AfiI CCNNNNNNNGG 2 cut(s) 323, 1160
AflII CTTAAG 1 cut(s) 40
AgeI ACCGGT 1 cut(s) 122
AgsI TTSAA 5 cut(s) 425, 523, 759, 785, 965
AjnI CCWGG 9 cut(s) 277, 322, 394, 454, 469, 484, 706, 873, 1233
AluBI AGCT 6 cut(s) 67, 410, 880, 1131, 1223, 1347
AluI AGCT 6 cut(s) 67, 410, 880, 1131, 1223, 1347
Alw21I GWGCWC 2 cut(s) 597, 882
Alw26I GTCTC 1 cut(s) 576
Alw44I GTGCAC 1 cut(s) 593
AlwI GGATC 1 cut(s) 656
AoxI GGCC 2 cut(s) 602, 932
ApaLI GTGCAC 1 cut(s) 593
ApeKI GCWGC 4 cut(s) 45, 58, 692, 1039
ApoI RAATTY 4 cut(s) 264, 1106, 1167, 1321
AsiGI ACCGGT 1 cut(s) 122
Asp700I GAANNNNTTC 1 cut(s) 1168
AspS9I GGNCC 7 cut(s) 236, 314, 603, 932, 971, 1261, 1271
AsuC2I CCSGG 3 cut(s) 104, 1265, 1276
AsuHPI GGTGA 4 cut(s) 137, 293, 818, 845
AsuNHI GCTAGC 1 cut(s) 611
AvaII GGWCC 5 cut(s) 236, 314, 971, 1261, 1271
BaeGI GKGCMC 2 cut(s) 597, 662
BanI GGYRCC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 882
BarI GAAGNNNNNNTAC 2 cut(s) 1028, 1060
Bbv12I GWGCWC 2 cut(s) 597, 882
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 4 cut(s) 57, 70, 679, 1051
BccI CCATC 9 cut(s) 139, 254, 514, 628, 824, 896, 922, 1097, 1310
BceAI ACGGC 1 cut(s) 617
BciT130I CCWGG 9 cut(s) 279, 324, 396, 456, 471, 486, 708, 875, 1235
BciVI GTATCC 1 cut(s) 1106
BcnI CCSGG 3 cut(s) 104, 1265, 1276
BcoDI GTCTC 1 cut(s) 576
BfaI CTAG 4 cut(s) 612, 1049, 1208, 1344
BfmI CTRYAG 1 cut(s) 186
BfrI CTTAAG 1 cut(s) 40
BfuI GTATCC 1 cut(s) 1106
BisI GCNGC 4 cut(s) 46, 59, 693, 1040
BlpI GCTNAGC 1 cut(s) 1348
BlsI GCNGC 4 cut(s) 47, 60, 694, 1041
BmcAI AGTACT 1 cut(s) 1080
Bme18I GGWCC 5 cut(s) 236, 314, 971, 1261, 1271
BmgT120I GGNCC 7 cut(s) 236, 314, 603, 932, 971, 1261, 1271
BmiI GGNNCC 8 cut(s) 6, 89, 315, 933, 973, 1263, 1273, 1340
BmsI GCATC 2 cut(s) 1023, 1042
BmtI GCTAGC 1 cut(s) 615
BpmI CTGGAG 2 cut(s) 180, 896
Bpu10I CCTNAGC 1 cut(s) 111
Bpu1102I GCTNAGC 1 cut(s) 1348
BpuEI CTTGAG 2 cut(s) 572, 866
BpuMI CCSGG 3 cut(s) 104, 1265, 1276
BsaJI CCNNGG 9 cut(s) 8, 53, 102, 469, 470, 484, 606, 640, 1274
BsaWI WCCGGW 1 cut(s) 122
BsaXI ACNNNNNCTCC 2 cut(s) 869, 899
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 1160
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 3 cut(s) 163, 773, 808
BseBI CCWGG 9 cut(s) 279, 324, 396, 456, 471, 486, 708, 875, 1235
BseDI CCNNGG 9 cut(s) 8, 53, 102, 469, 470, 484, 606, 640, 1274
BseGI GGATG 2 cut(s) 265, 907
BseLI CCNNNNNNNGG 2 cut(s) 323, 1160
BseMII CTCAG 2 cut(s) 102, 244
BseNI ACTGG 3 cut(s) 163, 773, 808
BseRI GAGGAG 2 cut(s) 435, 930
BseSI GKGCMC 2 cut(s) 597, 662
BseXI GCAGC 4 cut(s) 57, 70, 679, 1051
BsgI GTGCAG 1 cut(s) 1095
BshFI GGCC 2 cut(s) 604, 934
BshNI GGYRCC 1 cut(s) 87
BshTI ACCGGT 1 cut(s) 122
BsiHKAI GWGCWC 2 cut(s) 597, 882
BsiSI CCGG 4 cut(s) 104, 123, 1265, 1276
BslFI GGGAC 3 cut(s) 957, 1247, 1257
BslI CCNNNNNNNGG 2 cut(s) 323, 1160
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 3 cut(s) 957, 1247, 1257
BsmI GAATGC 2 cut(s) 842, 1090
BsnI GGCC 2 cut(s) 604, 934
Bsp1286I GDGCHC 3 cut(s) 597, 662, 882
Bsp143I GATC 2 cut(s) 283, 648
Bsp1720I GCTNAGC 1 cut(s) 1348
Bsp19I CCATGG 1 cut(s) 53
BspANI GGCC 2 cut(s) 604, 934
BspCNI CTCAG 2 cut(s) 103, 243
BspLI GGNNCC 8 cut(s) 6, 89, 315, 933, 973, 1263, 1273, 1340
BspMAI CTGCAG 1 cut(s) 190
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 1 cut(s) 656
BspT107I GGYRCC 1 cut(s) 87
BspTI CTTAAG 1 cut(s) 40
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 3 cut(s) 163, 773, 808
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 9 cut(s) 8, 53, 102, 469, 470, 484, 606, 640, 1274
BssMI GATC 2 cut(s) 283, 648
BssNAI GTATAC 1 cut(s) 766
BssT1I CCWWGG 3 cut(s) 53, 606, 640
Bst1107I GTATAC 1 cut(s) 766
Bst2UI CCWGG 9 cut(s) 279, 324, 396, 456, 471, 486, 708, 875, 1235
Bst4CI ACNGT 3 cut(s) 83, 220, 1060
Bst6I CTCTTC 4 cut(s) 78, 324, 706, 948
BstAFI CTTAAG 1 cut(s) 40
BstAPI GCANNNNNTGC 1 cut(s) 1061
BstC8I GCNNGC 2 cut(s) 613, 1074
BstDEI CTNAG 5 cut(s) 111, 230, 746, 797, 1348
BstDSI CCRYGG 1 cut(s) 53
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 2 cut(s) 265, 907
BstKTI GATC 2 cut(s) 286, 651
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 2 cut(s) 283, 648
BstMWI GCNNNNNNNGC 8 cut(s) 64, 407, 601, 689, 1061, 1155, 1193, 1208
BstNI CCWGG 9 cut(s) 279, 324, 396, 456, 471, 486, 708, 875, 1235
BstNSI RCATGY 1 cut(s) 814
BstSFI CTRYAG 1 cut(s) 186
BstSLI GKGCMC 2 cut(s) 597, 662
BstV1I GCAGC 4 cut(s) 57, 70, 679, 1051
BstXI CCANNNNNNTGG 1 cut(s) 395
BstZ17I GTATAC 1 cut(s) 766
BsuI GTATCC 1 cut(s) 1106
BsuRI GGCC 2 cut(s) 604, 934
BtgI CCRYGG 1 cut(s) 53
BtsCI GGATG 2 cut(s) 265, 907
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 4 cut(s) 238, 690, 801, 1024
Cac8I GCNNGC 2 cut(s) 613, 1074
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 7 cut(s) 236, 314, 603, 932, 971, 1261, 1271
CseI GACGC 2 cut(s) 625, 1193
Csp6I GTAC 2 cut(s) 1079, 1308
CspAI ACCGGT 1 cut(s) 122
CviAII CATG 5 cut(s) 54, 811, 947, 1146, 1155
CviQI GTAC 2 cut(s) 1079, 1308
DdeI CTNAG 5 cut(s) 111, 230, 746, 797, 1348
DpnI GATC 2 cut(s) 285, 650
DpnII GATC 2 cut(s) 283, 648
Eam1104I CTCTTC 4 cut(s) 78, 324, 706, 948
EarI CTCTTC 4 cut(s) 78, 324, 706, 948
Ecl136II GAGCTC 1 cut(s) 880
Eco130I CCWWGG 3 cut(s) 53, 606, 640
Eco24I GRGCYC 1 cut(s) 882
Eco47I GGWCC 5 cut(s) 236, 314, 971, 1261, 1271
Eco53kI GAGCTC 1 cut(s) 880
EcoICRI GAGCTC 1 cut(s) 880
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoO109I RGGNCCY 3 cut(s) 314, 971, 1261
EcoRI GAATTC 1 cut(s) 264
EcoRII CCWGG 9 cut(s) 277, 322, 394, 454, 469, 484, 706, 873, 1233
EcoT14I CCWWGG 3 cut(s) 53, 606, 640
EcoT38I GRGCYC 1 cut(s) 882
ErhI CCWWGG 3 cut(s) 53, 606, 640
FaeI CATG 5 cut(s) 57, 814, 950, 1149, 1158
FalI AAGNNNNNCTT 2 cut(s) 903, 935
FaqI GGGAC 3 cut(s) 957, 1247, 1257
FatI CATG 5 cut(s) 53, 810, 946, 1145, 1154
FauNDI CATATG 1 cut(s) 527
FblI GTMKAC 3 cut(s) 192, 765, 1182
Fnu4HI GCNGC 4 cut(s) 46, 59, 693, 1040
FokI GGATG 2 cut(s) 272, 914
FriOI GRGCYC 1 cut(s) 882
Fsp4HI GCNGC 4 cut(s) 46, 59, 693, 1040
FspBI CTAG 4 cut(s) 612, 1049, 1208, 1344
GluI GCNGC 4 cut(s) 46, 59, 693, 1040
GsuI CTGGAG 2 cut(s) 180, 896
HaeIII GGCC 2 cut(s) 604, 934
HapII CCGG 4 cut(s) 104, 123, 1265, 1276
HgaI GACGC 2 cut(s) 625, 1193
Hin1II CATG 5 cut(s) 57, 814, 950, 1149, 1158
HincII GTYRAC 2 cut(s) 477, 1183
HindII GTYRAC 2 cut(s) 477, 1183
HinfI GANTC 7 cut(s) 13, 182, 465, 564, 742, 952, 987
HpaI GTTAAC 1 cut(s) 477
HpaII CCGG 4 cut(s) 104, 123, 1265, 1276
HphI GGTGA 4 cut(s) 137, 293, 818, 845
Hpy166II GTNNAC 6 cut(s) 193, 236, 477, 595, 766, 1183
Hpy188I TCNGA 5 cut(s) 12, 253, 381, 576, 994
Hpy188III TCNNGA 5 cut(s) 118, 520, 665, 739, 1173
Hpy8I GTNNAC 6 cut(s) 193, 236, 477, 595, 766, 1183
Hpy99I CGWCG 3 cut(s) 514, 980, 1187
HpyAV CCTTC 4 cut(s) 419, 779, 779, 1145
HpyCH4III ACNGT 3 cut(s) 83, 220, 1060
HpyCH4IV ACGT 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 8 cut(s) 64, 407, 601, 689, 1061, 1155, 1193, 1208
HpyF3I CTNAG 5 cut(s) 111, 230, 746, 797, 1348
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 5 cut(s) 57, 814, 950, 1149, 1158
KspAI GTTAAC 1 cut(s) 477
Kzo9I GATC 2 cut(s) 283, 648
LmnI GCTCC 4 cut(s) 10, 788, 877, 1192
Lsp1109I GCAGC 4 cut(s) 57, 70, 679, 1051
LweI GCATC 2 cut(s) 1023, 1042
MaeI CTAG 4 cut(s) 612, 1049, 1208, 1344
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 2 cut(s) 731, 806
MalI GATC 2 cut(s) 285, 650
MboI GATC 2 cut(s) 283, 648
MboII GAAGA 6 cut(s) 65, 278, 311, 723, 935, 1176
MfeI CAATTG 1 cut(s) 727
MhlI GDGCHC 3 cut(s) 597, 662, 882
MluCI AATT 8 cut(s) 264, 702, 727, 837, 891, 1106, 1167, 1321
MlyI GAGTC 3 cut(s) 7, 176, 573
MmeI TCCRAC 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 1168
MseI TTAA 3 cut(s) 41, 171, 476
MspCI CTTAAG 1 cut(s) 40
MspI CCGG 4 cut(s) 104, 123, 1265, 1276
MunI CAATTG 1 cut(s) 727
Mva1269I GAATGC 2 cut(s) 842, 1090
MvaI CCWGG 9 cut(s) 279, 324, 396, 456, 471, 486, 708, 875, 1235
MwoI GCNNNNNNNGC 8 cut(s) 64, 407, 601, 689, 1061, 1155, 1193, 1208
NciI CCSGG 3 cut(s) 104, 1265, 1276
NcoI CCATGG 1 cut(s) 53
NdeI CATATG 1 cut(s) 527
NdeII GATC 2 cut(s) 283, 648
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 5 cut(s) 57, 814, 950, 1149, 1158
NlaIV GGNNCC 8 cut(s) 6, 89, 315, 933, 973, 1263, 1273, 1340
NmuCI GTSAC 2 cut(s) 731, 806
NspI RCATGY 1 cut(s) 814
PasI CCCWGGG 1 cut(s) 470
PctI GAATGC 2 cut(s) 842, 1090
PdmI GAANNNNTTC 1 cut(s) 1168
PfeI GAWTC 4 cut(s) 465, 742, 952, 987
PfoI TCCNGGA 3 cut(s) 322, 706, 873
PinAI ACCGGT 1 cut(s) 122
PkrI GCNGC 4 cut(s) 47, 60, 694, 1041
PleI GAGTC 3 cut(s) 7, 176, 572
PpsI GAGTC 3 cut(s) 7, 176, 572
PpuMI RGGWCCY 3 cut(s) 314, 971, 1261
PsiI TTATAA 1 cut(s) 896
Psp124BI GAGCTC 1 cut(s) 882
Psp5II RGGWCCY 3 cut(s) 314, 971, 1261
Psp6I CCWGG 9 cut(s) 277, 322, 394, 454, 469, 484, 706, 873, 1233
PspGI CCWGG 9 cut(s) 277, 322, 394, 454, 469, 484, 706, 873, 1233
PspN4I GGNNCC 8 cut(s) 6, 89, 315, 933, 973, 1263, 1273, 1340
PspPI GGNCC 7 cut(s) 236, 314, 603, 932, 971, 1261, 1271
PspPPI RGGWCCY 3 cut(s) 314, 971, 1261
PstI CTGCAG 1 cut(s) 190
RsaI GTAC 2 cut(s) 1080, 1309
RsaNI GTAC 2 cut(s) 1079, 1308
SacI GAGCTC 1 cut(s) 882
SalI GTCGAC 1 cut(s) 1181
SaqAI TTAA 3 cut(s) 41, 171, 476
SatI GCNGC 4 cut(s) 46, 59, 693, 1040
Sau3AI GATC 2 cut(s) 283, 648
Sau96I GGNCC 7 cut(s) 236, 314, 603, 932, 971, 1261, 1271
ScaI AGTACT 1 cut(s) 1080
SchI GAGTC 3 cut(s) 7, 176, 573
SduI GDGCHC 3 cut(s) 597, 662, 882
SfaNI GCATC 2 cut(s) 1023, 1042
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 5 cut(s) 236, 314, 971, 1261, 1271
SmlI CTYRAG 3 cut(s) 40, 551, 881
SmoI CTYRAG 3 cut(s) 40, 551, 881
Sse9I AATT 8 cut(s) 264, 702, 727, 837, 891, 1106, 1167, 1321
SspMI CTAG 4 cut(s) 612, 1049, 1208, 1344
SstI GAGCTC 1 cut(s) 882
StyI CCWWGG 3 cut(s) 53, 606, 640
TaaI ACNGT 3 cut(s) 83, 220, 1060
TaiI ACGT 1 cut(s) 198
TaqI TCGA 5 cut(s) 509, 978, 1092, 1182, 1243
TaqII GACCGA 1 cut(s) 253
TasI AATT 8 cut(s) 264, 702, 727, 837, 891, 1106, 1167, 1321
TatI WGTACW 2 cut(s) 1078, 1307
TfiI GAWTC 4 cut(s) 465, 742, 952, 987
Tru1I TTAA 3 cut(s) 41, 171, 476
Tru9I TTAA 3 cut(s) 41, 171, 476
TscAI CASTG 4 cut(s) 238, 690, 808, 1024
TseFI GTSAC 2 cut(s) 731, 806
TseI GCWGC 4 cut(s) 45, 58, 692, 1039
Tsp45I GTSAC 2 cut(s) 731, 806
TspDTI ATGAA 5 cut(s) 212, 345, 769, 923, 935
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 4 cut(s) 238, 690, 808, 1024
Vha464I CTTAAG 1 cut(s) 40
VneI GTGCAC 1 cut(s) 593
VpaK11BI GGWCC 5 cut(s) 236, 314, 971, 1261, 1271
XagI CCTNNNNNAGG 1 cut(s) 321
XapI RAATTY 4 cut(s) 264, 1106, 1167, 1321
XceI RCATGY 1 cut(s) 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 330
XmiI GTMKAC 3 cut(s) 192, 765, 1182
XmnI GAANNNNTTC 1 cut(s) 1168
XspI CTAG 4 cut(s) 612, 1049, 1208, 1344
ZrmI AGTACT 1 cut(s) 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.