MD15G1294800.v1.1

formamidase C869.04 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003336
Physical Location & Seq
Forward (+)
27575861 .. 27579832
3972 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1294800.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGGCCTTCTATGGACCGAGACTGGTTGTGCCTATAGACGTGAAGAAGAAGCCATGGGAACAGAAGCTTCCTCTTCACAATCGGTGGCACCCGGACATACCACCGGTGGCAGAAGTGGCAGCTGGAGAGGTTTTTCGTGTCGAGATGGTGGATTTCAGTGGTGGTGGGATTACCAAAGAGTACACTGCAGAGGACATCAAACATTCCAATCCCTCCATTGTTCATTACCTCAGTGGGCCGATTAGAATTTCGGATTCCGATGGAATTCCAGCACAGCCAGGCGATCTTCTGGTGGTTGAGATATGCAACTTGGGTCCTCTCCCAGGAGATGAATGGGGTTTCACTGCGACATTTGACAGAGAAAATGGAGGGGGTTTTCTGACTGACCATTTTCCTTCTGCAACCAAAGCTATCTGGTATTTCGAAGGGATATATGCCTACTCGCCTCACATACCAGGAGTGAGATTCCCAGGGTTAACCCACCCCGGAATAGTTGGAACAGCACCATCAATGGAACTCCTGAAAATATGGAATGAAAGGGAGAGGGAACTTGAAGAAAATGGACTCAAGACTATGAAATTATGTGAGGTTTTGCATCAACGACCCTTGGCTAACCTACCATCAACAAAAGGTTGCGTCCTTGGAGGGATCAAAGAGGGCACTCCTGAATGGGAAAAGATCGCCATGGAGGCTGCAAGAACCATTCCAGGAAGAGAAAATGGAGGCAATTGTGACATCAAAAATCTTAGTCGTGGTTCAAAGGTATACCTTCCAGTATTCGTTGAAGGAGCAAATCTTAGTACTGGTGACATGCACTTTTCCCAGGGTGATGGAGAAATTTCATTCTGCGGGGCAATTGAGATGAGCGGATTCCTGGAGCTCAAGTGCGAAATTATAAGGGGAGGAATGAAAGAATACCTTACACCAATGGGGCCCACACCTCTTCACGTAAACCCAATCTTTGAGATAGGCCCGGTCGAGCCAAGATTCTCAGAGTGGTTGGTATTTGAGGGCATCAGTGTTGACGAGAGGGGGAAGCAGCATTACCTAGATGCAACTGTGGCGTACAAGCGTGCAGTACTCAATGCTATTGACTACCTCTCTAAATTTGGATACTCCAAAGAACAGAGCTACCTTCTGTTGTCATGCTGCCCGTGTGAGGGAAGAATTTCCGGTATAGTTGACTCTCCCAATGCTGTTGCAACCCTAGCAATCCCAACAGCTATCTTTGACCAGGATATTCGCCCAAAAGCAAACAAGGTGCCTGTTGGTCCCCGGATAGTGAGGAAACCAGATGTCCTAAAATGTAGTTATGATGGAAATTTGCCAACGACAAGGAACCCTAGCTCTGCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

453

Amino Acids

49.65

Weight (kDa)

5.65

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 12 - 412 4.7e-167 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 896
AccB1I GGYRCC 2 cut(s) 87, 1261
AccBSI CCGCTC 1 cut(s) 867
AccI GTMKAC 1 cut(s) 765
AciI CCGC 2 cut(s) 849, 867
AclWI GGATC 1 cut(s) 656
AcsI RAATTY 6 cut(s) 246, 264, 837, 1106, 1167, 1321
AfaI GTAC 4 cut(s) 182, 802, 1067, 1080
AfiI CCNNNNNNNGG 3 cut(s) 323, 1159, 1160
AgeI ACCGGT 1 cut(s) 103
AgsI TTSAA 3 cut(s) 554, 759, 785
AjiI CACGTC 1 cut(s) 40
AjnI CCWGG 8 cut(s) 277, 322, 454, 469, 706, 822, 873, 1233
AluBI AGCT 7 cut(s) 67, 122, 410, 880, 1131, 1223, 1347
AluI AGCT 7 cut(s) 67, 122, 410, 880, 1131, 1223, 1347
Alw21I GWGCWC 1 cut(s) 882
Alw26I GTCTC 1 cut(s) 13
AlwI GGATC 1 cut(s) 656
AoxI GGCC 4 cut(s) 3, 236, 932, 970
ApaI GGGCCC 1 cut(s) 936
ApeKI GCWGC 4 cut(s) 119, 692, 1039, 1149
ApoI RAATTY 6 cut(s) 246, 264, 837, 1106, 1167, 1321
AsiGI ACCGGT 1 cut(s) 103
Asp700I GAANNNNTTC 1 cut(s) 1168
AspS9I GGNCC 7 cut(s) 14, 236, 314, 932, 933, 971, 1271
AsuC2I CCSGG 4 cut(s) 92, 486, 974, 1276
AsuHPI GGTGA 2 cut(s) 818, 839
AsuII TTCGAA 1 cut(s) 423
AvaII GGWCC 3 cut(s) 14, 314, 1271
BaeGI GKGCMC 2 cut(s) 662, 936
BanI GGYRCC 2 cut(s) 87, 1261
BanII GRGCYC 2 cut(s) 882, 936
BarI GAAGNNNNNNTAC 2 cut(s) 1028, 1060
Bbv12I GWGCWC 1 cut(s) 882
BbvI GCAGC 4 cut(s) 131, 679, 1051, 1136
BccI CCATC 6 cut(s) 139, 254, 514, 628, 824, 1310
BciT130I CCWGG 8 cut(s) 279, 324, 456, 471, 708, 824, 875, 1235
BciVI GTATCC 1 cut(s) 1106
BcnI CCSGG 4 cut(s) 92, 486, 974, 1276
BcoDI GTCTC 1 cut(s) 13
BfaI CTAG 3 cut(s) 1049, 1208, 1344
BfmI CTRYAG 2 cut(s) 33, 186
BfuI GTATCC 1 cut(s) 1106
BglI GCCNNNNNGGC 1 cut(s) 689
BisI GCNGC 4 cut(s) 120, 693, 1040, 1150
BlsI GCNGC 4 cut(s) 121, 694, 1041, 1151
BmcAI AGTACT 2 cut(s) 802, 1080
Bme18I GGWCC 3 cut(s) 14, 314, 1271
BmgBI CACGTC 1 cut(s) 40
BmgT120I GGNCC 7 cut(s) 14, 236, 314, 932, 933, 971, 1271
BmiI GGNNCC 7 cut(s) 89, 315, 933, 934, 1263, 1273, 1340
BmsI GCATC 3 cut(s) 604, 1023, 1042
BpmI CTGGAG 2 cut(s) 144, 896
Bpu14I TTCGAA 1 cut(s) 423
BpuEI CTTGAG 2 cut(s) 551, 866
BpuMI CCSGG 4 cut(s) 92, 486, 974, 1276
BsaAI YACGTR 1 cut(s) 949
BsaWI WCCGGW 2 cut(s) 103, 1172
BsaXI ACNNNNNCTCC 2 cut(s) 869, 899
Bsc4I CCNNNNNNNGG 3 cut(s) 323, 1159, 1160
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 3 cut(s) 27, 773, 808
BseBI CCWGG 8 cut(s) 279, 324, 456, 471, 708, 824, 875, 1235
BseLI CCNNNNNNNGG 3 cut(s) 323, 1159, 1160
BseMII CTCAG 2 cut(s) 244, 1005
BseNI ACTGG 3 cut(s) 27, 773, 808
BseSI GKGCMC 2 cut(s) 662, 936
BseXI GCAGC 4 cut(s) 131, 679, 1051, 1136
BsgI GTGCAG 1 cut(s) 1095
Bsh1285I CGRYCG 1 cut(s) 978
BshFI GGCC 4 cut(s) 5, 238, 934, 972
BshNI GGYRCC 2 cut(s) 87, 1261
BshTI ACCGGT 1 cut(s) 103
BsiEI CGRYCG 1 cut(s) 978
BsiHKAI GWGCWC 1 cut(s) 882
BsiSI CCGG 6 cut(s) 92, 104, 486, 974, 1173, 1276
BslFI GGGAC 1 cut(s) 1257
BslI CCNNNNNNNGG 3 cut(s) 323, 1159, 1160
BsmAI GTCTC 1 cut(s) 13
BsmFI GGGAC 1 cut(s) 1257
BsnI GGCC 4 cut(s) 5, 238, 934, 972
Bsp119I TTCGAA 1 cut(s) 423
Bsp120I GGGCCC 1 cut(s) 932
Bsp1286I GDGCHC 3 cut(s) 662, 882, 936
Bsp143I GATC 3 cut(s) 283, 648, 678
Bsp19I CCATGG 2 cut(s) 53, 684
BspACI CCGC 2 cut(s) 849, 867
BspANI GGCC 4 cut(s) 5, 238, 934, 972
BspCNI CTCAG 2 cut(s) 243, 1004
BspLI GGNNCC 7 cut(s) 89, 315, 933, 934, 1263, 1273, 1340
BspMAI CTGCAG 1 cut(s) 190
BspPI GGATC 1 cut(s) 656
BspT104I TTCGAA 1 cut(s) 423
BspT107I GGYRCC 2 cut(s) 87, 1261
BsrBI CCGCTC 1 cut(s) 867
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 3 cut(s) 27, 773, 808
BssAI RCCGGY 1 cut(s) 103
BssMI GATC 3 cut(s) 283, 648, 678
BssNAI GTATAC 1 cut(s) 766
BssT1I CCWWGG 4 cut(s) 53, 606, 640, 684
Bst1107I GTATAC 1 cut(s) 766
Bst2UI CCWGG 8 cut(s) 279, 324, 456, 471, 708, 824, 875, 1235
Bst4CI ACNGT 1 cut(s) 1060
Bst6I CTCTTC 3 cut(s) 78, 706, 948
BstBAI YACGTR 1 cut(s) 949
BstBI TTCGAA 1 cut(s) 423
BstC8I GCNNGC 1 cut(s) 1074
BstDEI CTNAG 4 cut(s) 230, 746, 797, 991
BstDSI CCRYGG 2 cut(s) 53, 684
BstENI CCTNNNNNAGG 1 cut(s) 321
BstKTI GATC 3 cut(s) 286, 651, 681
BstMAI GTCTC 1 cut(s) 13
BstMBI GATC 3 cut(s) 283, 648, 678
BstMCI CGRYCG 1 cut(s) 978
BstMWI GCNNNNNNNGC 5 cut(s) 116, 407, 689, 1061, 1208
BstNI CCWGG 8 cut(s) 279, 324, 456, 471, 708, 824, 875, 1235
BstNSI RCATGY 1 cut(s) 814
BstSFI CTRYAG 2 cut(s) 33, 186
BstSLI GKGCMC 2 cut(s) 662, 936
BstV1I GCAGC 4 cut(s) 131, 679, 1051, 1136
BstXI CCANNNNNNTGG 1 cut(s) 830
BstZ17I GTATAC 1 cut(s) 766
BsuI GTATCC 1 cut(s) 1106
BsuRI GGCC 4 cut(s) 5, 238, 934, 972
BtgI CCRYGG 2 cut(s) 53, 684
BtrI CACGTC 1 cut(s) 40
BtsI GCAGTG 2 cut(s) 183, 342
BtsIMutI CAGTG 5 cut(s) 163, 183, 238, 342, 1024
Cac8I GCNNGC 1 cut(s) 1074
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 7 cut(s) 14, 236, 314, 932, 933, 971, 1271
CseI GACGC 1 cut(s) 625
Csp6I GTAC 4 cut(s) 181, 801, 1066, 1079
CspAI ACCGGT 1 cut(s) 103
CviAII CATG 4 cut(s) 54, 685, 811, 1146
CviQI GTAC 4 cut(s) 181, 801, 1066, 1079
DdeI CTNAG 4 cut(s) 230, 746, 797, 991
DpnI GATC 3 cut(s) 285, 650, 680
DpnII GATC 3 cut(s) 283, 648, 678
Eam1104I CTCTTC 3 cut(s) 78, 706, 948
EarI CTCTTC 3 cut(s) 78, 706, 948
Ecl136II GAGCTC 1 cut(s) 880
Eco130I CCWWGG 4 cut(s) 53, 606, 640, 684
Eco24I GRGCYC 2 cut(s) 882, 936
Eco47I GGWCC 3 cut(s) 14, 314, 1271
Eco53kI GAGCTC 1 cut(s) 880
EcoICRI GAGCTC 1 cut(s) 880
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoO109I RGGNCCY 2 cut(s) 314, 932
EcoRI GAATTC 1 cut(s) 264
EcoRII CCWGG 8 cut(s) 277, 322, 454, 469, 706, 822, 873, 1233
EcoT14I CCWWGG 4 cut(s) 53, 606, 640, 684
EcoT38I GRGCYC 2 cut(s) 882, 936
ErhI CCWWGG 4 cut(s) 53, 606, 640, 684
FaeI CATG 4 cut(s) 57, 688, 814, 1149
FalI AAGNNNNNCTT 2 cut(s) 903, 935
FaqI GGGAC 1 cut(s) 1257
FatI CATG 4 cut(s) 53, 684, 810, 1145
FauI CCCGC 1 cut(s) 842
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 4 cut(s) 120, 693, 1040, 1150
FriOI GRGCYC 2 cut(s) 882, 936
Fsp4HI GCNGC 4 cut(s) 120, 693, 1040, 1150
FspBI CTAG 3 cut(s) 1049, 1208, 1344
GluI GCNGC 4 cut(s) 120, 693, 1040, 1150
GsuI CTGGAG 2 cut(s) 144, 896
HaeIII GGCC 4 cut(s) 5, 238, 934, 972
HapII CCGG 6 cut(s) 92, 104, 486, 974, 1173, 1276
HgaI GACGC 1 cut(s) 625
Hin1II CATG 4 cut(s) 57, 688, 814, 1149
HincII GTYRAC 3 cut(s) 477, 1024, 1183
HindII GTYRAC 3 cut(s) 477, 1024, 1183
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 6 cut(s) 254, 465, 564, 870, 987, 1184
HpaI GTTAAC 1 cut(s) 477
HpaII CCGG 6 cut(s) 92, 104, 486, 974, 1173, 1276
HphI GGTGA 2 cut(s) 818, 839
Hpy166II GTNNAC 6 cut(s) 183, 477, 766, 952, 1024, 1183
Hpy188I TCNGA 4 cut(s) 253, 259, 381, 994
Hpy188III TCNNGA 4 cut(s) 142, 520, 568, 665
Hpy8I GTNNAC 6 cut(s) 183, 477, 766, 952, 1024, 1183
HpyAV CCTTC 6 cut(s) 16, 405, 419, 779, 779, 1145
HpyCH4III ACNGT 1 cut(s) 1060
HpyCH4IV ACGT 2 cut(s) 39, 948
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 407, 689, 1061, 1208
HpyF3I CTNAG 4 cut(s) 230, 746, 797, 991
HpySE526I ACGT 2 cut(s) 39, 948
Hsp92II CATG 4 cut(s) 57, 688, 814, 1149
KspAI GTTAAC 1 cut(s) 477
Kzo9I GATC 3 cut(s) 283, 648, 678
LmnI GCTCC 2 cut(s) 788, 877
Lsp1109I GCAGC 4 cut(s) 131, 679, 1051, 1136
LweI GCATC 3 cut(s) 604, 1023, 1042
MaeI CTAG 3 cut(s) 1049, 1208, 1344
MaeII ACGT 2 cut(s) 39, 948
MaeIII GTNAC 2 cut(s) 731, 806
MalI GATC 3 cut(s) 285, 650, 680
MbiI CCGCTC 1 cut(s) 867
MboI GATC 3 cut(s) 283, 648, 678
MboII GAAGA 8 cut(s) 55, 58, 65, 278, 566, 723, 935, 1176
MfeI CAATTG 2 cut(s) 727, 855
MhlI GDGCHC 3 cut(s) 662, 882, 936
MlyI GAGTC 2 cut(s) 558, 1178
MmeI TCCRAC 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 1168
MseI TTAA 1 cut(s) 476
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 6 cut(s) 92, 104, 486, 974, 1173, 1276
MunI CAATTG 2 cut(s) 727, 855
MvaI CCWGG 8 cut(s) 279, 324, 456, 471, 708, 824, 875, 1235
MwoI GCNNNNNNNGC 5 cut(s) 116, 407, 689, 1061, 1208
NciI CCSGG 4 cut(s) 92, 486, 974, 1276
NcoI CCATGG 2 cut(s) 53, 684
NdeII GATC 3 cut(s) 283, 648, 678
NlaIII CATG 4 cut(s) 57, 688, 814, 1149
NlaIV GGNNCC 7 cut(s) 89, 315, 933, 934, 1263, 1273, 1340
NmuCI GTSAC 2 cut(s) 731, 806
NspI RCATGY 1 cut(s) 814
NspV TTCGAA 1 cut(s) 423
PasI CCCWGGG 2 cut(s) 470, 823
PdmI GAANNNNTTC 1 cut(s) 1168
PfeI GAWTC 4 cut(s) 254, 465, 870, 987
PfoI TCCNGGA 2 cut(s) 706, 873
PinAI ACCGGT 1 cut(s) 103
PkrI GCNGC 4 cut(s) 121, 694, 1041, 1151
PleI GAGTC 2 cut(s) 558, 1178
PpsI GAGTC 2 cut(s) 558, 1178
Ppu21I YACGTR 1 cut(s) 949
PpuMI RGGWCCY 1 cut(s) 314
PsiI TTATAA 1 cut(s) 896
Psp124BI GAGCTC 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 314
Psp6I CCWGG 8 cut(s) 277, 322, 454, 469, 706, 822, 873, 1233
PspGI CCWGG 8 cut(s) 277, 322, 454, 469, 706, 822, 873, 1233
PspN4I GGNNCC 7 cut(s) 89, 315, 933, 934, 1263, 1273, 1340
PspOMI GGGCCC 1 cut(s) 932
PspPI GGNCC 7 cut(s) 14, 236, 314, 932, 933, 971, 1271
PspPPI RGGWCCY 1 cut(s) 314
PstI CTGCAG 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 4 cut(s) 182, 802, 1067, 1080
RsaNI GTAC 4 cut(s) 181, 801, 1066, 1079
SacI GAGCTC 1 cut(s) 882
SaqAI TTAA 1 cut(s) 476
SatI GCNGC 4 cut(s) 120, 693, 1040, 1150
Sau3AI GATC 3 cut(s) 283, 648, 678
Sau96I GGNCC 7 cut(s) 14, 236, 314, 932, 933, 971, 1271
ScaI AGTACT 2 cut(s) 802, 1080
SchI GAGTC 2 cut(s) 558, 1178
SduI GDGCHC 3 cut(s) 662, 882, 936
SfaNI GCATC 3 cut(s) 604, 1023, 1042
SfcI CTRYAG 2 cut(s) 33, 186
SfuI TTCGAA 1 cut(s) 423
SgrAI CRCCGGYG 1 cut(s) 103
SinI GGWCC 3 cut(s) 14, 314, 1271
SmlI CTYRAG 2 cut(s) 566, 881
SmoI CTYRAG 2 cut(s) 566, 881
SsiI CCGC 2 cut(s) 849, 867
SspMI CTAG 3 cut(s) 1049, 1208, 1344
SstI GAGCTC 1 cut(s) 882
StyI CCWWGG 4 cut(s) 53, 606, 640, 684
TaaI ACNGT 1 cut(s) 1060
TaiI ACGT 2 cut(s) 42, 951
TaqI TCGA 3 cut(s) 141, 423, 978
TaqII GACCGA 1 cut(s) 31
TatI WGTACW 3 cut(s) 180, 800, 1078
TfiI GAWTC 4 cut(s) 254, 465, 870, 987
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TscAI CASTG 5 cut(s) 163, 190, 238, 349, 1024
TseFI GTSAC 2 cut(s) 731, 806
TseI GCWGC 4 cut(s) 119, 692, 1039, 1149
Tsp45I GTSAC 2 cut(s) 731, 806
TspDTI ATGAA 6 cut(s) 212, 345, 549, 590, 831, 923
TspRI CASTG 5 cut(s) 163, 190, 238, 349, 1024
VpaK11BI GGWCC 3 cut(s) 14, 314, 1271
XagI CCTNNNNNAGG 1 cut(s) 321
XapI RAATTY 6 cut(s) 246, 264, 837, 1106, 1167, 1321
XceI RCATGY 1 cut(s) 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 330
XmiI GTMKAC 1 cut(s) 765
XmnI GAANNNNTTC 1 cut(s) 1168
XspI CTAG 3 cut(s) 1049, 1208, 1344
ZrmI AGTACT 2 cut(s) 802, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.