AT4G37560

formamidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003336
Physical Location & Seq
Forward (+)
17646408 .. 17648684
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G37560.2

Sequence Viewer

Length: 1359 bp
ATGGCTCCAGCAAGTCCACGAGTGGTGGTGAAGGTGGATTTGAAGAAGAGGCCATGGCAGCAGACTCAGCCACTGCACAATAGATGGCATCCTGAGATACCATCAGTTGCAGAGGTCAAGACTGGTGAGTTGTTTAGGGTGGAAATGGTTGACTGGACGGGAGGTGCAGTCAAAGAAGATGGCTCTGCTGGCGATATTAAGAGCATAGACCTCTCTACTGTTCATTACCTGAGTGGGCCGATCAAAGTTGTGGATGAGGATGGTGTTGCAGCTAGGCCAGGTGATCTTCTTGCTGTTGAGATATGCAACTTGGGTCCTCTTCCAGGAGATGAATGGGGATTCACTGCTTCATTTGATAGAGAGAATGGCGGTGGTTTCTTGACTGACCATTTCCCTTGTGCGACCAAAGCTATTTGGTACTTTGAAGGGATTTATGCCTACTCTCCTCAAATTCCTGGAGTACGATTTCCAGGGTTGACACATCCAGGAATCATTGGCACTGCACCATCAAAAGAACTCTTGAGAATATGGAATGAAAGGGAAAGACAATTAGAAGAAAAAGGTGTTGAGTCCCTAACACTATGTGAGGTTGTGCATCAGCGGCCATTAGCAAGCCTACCAACAACTAAAGGATGTCTACTAGGAAATATTGAAGAGAGGACATCAGAATGGGAAAGAATTGCAAAGGAGGCTGCTAGGACAATACCAGGAAGGGAAAATGGAGGAAACTGTGACATAAAAAACCTAAGCAGTGGCTCTAAAATATATCTTCCAGTGTTTGTAGAAGGAGCTAATCTAAGCACCGGTGACATGCATTTCTCACAGGGTGATGGCGAGATCTCATTCTGTGGAGCCATAGAGATGAGTGGATTTCTAGAACTCAAGTGTGAAATCATTAGAAACGGGATGCAGGAGTACCTTACACCAATGGGACCAACCACTCTCCACGTAAACCCGATCTTTGAGATAGGGCCAGTGGAACCAAGATTCTCAGAGTGGCTTGTCTTCGAGGGTATCAGTGTCGATGAGAGCGGAAGACAACATTACCTAGATGCAACAGTTGCATACAAACGAGCAGTTCTCAACGCTATAGACTATCTCTTCAAATTCGGTTACTCAAAAGAACAGGTATATCTCTTACTCTCATGCTGTCCATGCGAAGGAAGAATATCCGGTATAGTCGATTCTCCAAATGCCGTTGCTACACTTGCAATACCCACTGCCATCTTTGATCAGGATATTCGTCCAAAGACCCGAAAAGTTCCAACAGGAGCTCGGATAGTGAAGAAGCCAGACGTTATGAAGAGCACATATGATGGAAAACTTCCAATTACAAAGAATTCAAGTTCTTCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
FmdA_AmdA

Protein Analysis

452

Amino Acids

49.7

Weight (kDa)

5.54

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FmdA_AmdA PF03069 11 - 412 1.1e-170 Acetamidase/Formamidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1032
AccI GTMKAC 1 cut(s) 637
AciI CCGC 3 cut(s) 369, 601, 1032
AcoI YGGCCR 1 cut(s) 602
AcsI RAATTY 3 cut(s) 450, 1106, 1339
AdeI CACNNNGTG 2 cut(s) 584, 827
AfaI GTAC 3 cut(s) 419, 462, 917
AfiI CCNNNNNNNGG 2 cut(s) 323, 1160
AgeI ACCGGT 1 cut(s) 803
AgsI TTSAA 5 cut(s) 43, 425, 653, 1105, 1344
AjnI CCWGG 6 cut(s) 277, 322, 454, 469, 484, 706
AluBI AGCT 4 cut(s) 272, 410, 791, 1274
AluI AGCT 4 cut(s) 272, 410, 791, 1274
Alw21I GWGCWC 2 cut(s) 1276, 1310
AlwNI CAGNNNCTG 1 cut(s) 73
AoxI GGCC 5 cut(s) 50, 236, 275, 602, 971
ApeKI GCWGC 3 cut(s) 58, 269, 692
ApoI RAATTY 3 cut(s) 450, 1106, 1339
AsiGI ACCGGT 1 cut(s) 803
AspS9I GGNCC 4 cut(s) 236, 314, 932, 971
AsuHPI GGTGA 5 cut(s) 40, 137, 293, 818, 839
AvaII GGWCC 2 cut(s) 314, 932
BanII GRGCYC 1 cut(s) 1276
BauI CACGAG 1 cut(s) 18
BbsI GAAGAC 2 cut(s) 997, 1042
Bbv12I GWGCWC 2 cut(s) 1276, 1310
BbvI GCAGC 3 cut(s) 70, 281, 679
BccI CCATC 8 cut(s) 78, 109, 173, 254, 514, 824, 1232, 1310
BceAI ACGGC 1 cut(s) 1181
BciT130I CCWGG 6 cut(s) 279, 324, 456, 471, 486, 708
BclI TGATCA 1 cut(s) 1231
BfaI CTAG 5 cut(s) 273, 641, 696, 875, 1049
BfmI CTRYAG 1 cut(s) 1089
BglII AGATCT 1 cut(s) 837
BisI GCNGC 4 cut(s) 59, 270, 602, 693
BlsI GCNGC 4 cut(s) 60, 271, 603, 694
Bme1390I CCNGG 6 cut(s) 279, 324, 456, 471, 486, 708
Bme18I GGWCC 2 cut(s) 314, 932
BmgT120I GGNCC 4 cut(s) 236, 314, 932, 971
BmiI GGNNCC 5 cut(s) 6, 315, 853, 933, 981
BmrFI CCNGG 6 cut(s) 279, 324, 456, 471, 486, 708
BmsI GCATC 4 cut(s) 97, 604, 897, 1042
BpiI GAAGAC 2 cut(s) 997, 1042
BplI GAGNNNNNCTC 2 cut(s) 1065, 1097
BpmI CTGGAG 1 cut(s) 477
Bpu10I CCTNAGC 1 cut(s) 746
BpuEI CTTGAG 2 cut(s) 541, 866
BsaAI YACGTR 1 cut(s) 949
BsaJI CCNNGG 2 cut(s) 53, 470
BsaWI WCCGGW 2 cut(s) 803, 1172
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 1160
Bse118I RCCGGY 1 cut(s) 803
Bse1I ACTGG 4 cut(s) 127, 158, 773, 974
BseBI CCWGG 6 cut(s) 279, 324, 456, 471, 486, 708
BseDI CCNNGG 2 cut(s) 53, 470
BseGI GGATG 6 cut(s) 88, 259, 265, 481, 638, 912
BseLI CCNNNNNNNGG 2 cut(s) 323, 1160
BseMII CTCAG 4 cut(s) 80, 84, 221, 1005
BseNI ACTGG 4 cut(s) 127, 158, 773, 974
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 3 cut(s) 70, 281, 679
BsgI GTGCAG 3 cut(s) 59, 186, 486
BshFI GGCC 5 cut(s) 52, 238, 277, 604, 973
BshTI ACCGGT 1 cut(s) 803
BsiHKAI GWGCWC 2 cut(s) 1276, 1310
BsiSI CCGG 2 cut(s) 804, 1173
BslFI GGGAC 2 cut(s) 556, 945
BslI CCNNNNNNNGG 2 cut(s) 323, 1160
BsmFI GGGAC 2 cut(s) 556, 945
BsnI GGCC 5 cut(s) 52, 238, 277, 604, 973
Bsp1286I GDGCHC 2 cut(s) 1276, 1310
Bsp143I GATC 5 cut(s) 240, 283, 837, 957, 1231
Bsp19I CCATGG 1 cut(s) 53
BspACI CCGC 3 cut(s) 369, 601, 1032
BspANI GGCC 5 cut(s) 52, 238, 277, 604, 973
BspCNI CTCAG 4 cut(s) 79, 85, 222, 1004
BspLI GGNNCC 5 cut(s) 6, 315, 853, 933, 981
BspQI GCTCTTC 1 cut(s) 1298
BsrBI CCGCTC 1 cut(s) 1032
BsrFI RCCGGY 1 cut(s) 803
BsrI ACTGG 4 cut(s) 127, 158, 773, 974
BssAI RCCGGY 1 cut(s) 803
BssECI CCNNGG 2 cut(s) 53, 470
BssMI GATC 5 cut(s) 240, 283, 837, 957, 1231
BssSI CACGAG 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 53
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 6 cut(s) 279, 324, 456, 471, 486, 708
Bst4CI ACNGT 3 cut(s) 220, 731, 1060
Bst6I CTCTTC 5 cut(s) 41, 324, 648, 1106, 1298
BstAPI GCANNNNNTGC 1 cut(s) 1061
BstBAI YACGTR 1 cut(s) 949
BstC8I GCNNGC 2 cut(s) 190, 613
BstDEI CTNAG 7 cut(s) 66, 93, 230, 746, 797, 991, 1356
BstDSI CCRYGG 1 cut(s) 53
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 6 cut(s) 88, 259, 265, 481, 638, 912
BstKTI GATC 5 cut(s) 243, 286, 840, 960, 1234
BstMBI GATC 5 cut(s) 240, 283, 837, 957, 1231
BstMWI GCNNNNNNNGC 9 cut(s) 58, 67, 189, 407, 601, 689, 1061, 1155, 1208
BstNI CCWGG 6 cut(s) 279, 324, 456, 471, 486, 708
BstNSI RCATGY 1 cut(s) 814
BstSCI CCNGG 6 cut(s) 277, 322, 454, 469, 484, 706
BstSFI CTRYAG 1 cut(s) 1089
BstV1I GCAGC 3 cut(s) 70, 281, 679
BstV2I GAAGAC 2 cut(s) 997, 1042
BstX2I RGATCY 1 cut(s) 837
BstYI RGATCY 1 cut(s) 837
BsuRI GGCC 5 cut(s) 52, 238, 277, 604, 973
BtgI CCRYGG 1 cut(s) 53
BtsCI GGATG 6 cut(s) 88, 259, 265, 481, 638, 912
BtsI GCAGTG 5 cut(s) 71, 342, 498, 757, 1218
BtsIMutI CAGTG 8 cut(s) 71, 342, 498, 757, 780, 981, 1024, 1218
Cac8I GCNNGC 2 cut(s) 190, 613
CaiI CAGNNNCTG 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 803
Cfr13I GGNCC 4 cut(s) 236, 314, 932, 971
Csp6I GTAC 3 cut(s) 418, 461, 916
CspAI ACCGGT 1 cut(s) 803
CviAII CATG 4 cut(s) 54, 811, 1146, 1155
CviQI GTAC 3 cut(s) 418, 461, 916
DdeI CTNAG 7 cut(s) 66, 93, 230, 746, 797, 991, 1356
DpnI GATC 5 cut(s) 242, 285, 839, 959, 1233
DpnII GATC 5 cut(s) 240, 283, 837, 957, 1231
DraIII CACNNNGTG 2 cut(s) 584, 827
EaeI YGGCCR 1 cut(s) 602
Eam1104I CTCTTC 5 cut(s) 41, 324, 648, 1106, 1298
EarI CTCTTC 5 cut(s) 41, 324, 648, 1106, 1298
Ecl136II GAGCTC 1 cut(s) 1274
Eco130I CCWWGG 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 1276
Eco47I GGWCC 2 cut(s) 314, 932
Eco53kI GAGCTC 1 cut(s) 1274
EcoICRI GAGCTC 1 cut(s) 1274
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 1339
EcoRII CCWGG 6 cut(s) 277, 322, 454, 469, 484, 706
EcoT14I CCWWGG 1 cut(s) 53
EcoT22I ATGCAT 1 cut(s) 816
EcoT38I GRGCYC 1 cut(s) 1276
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 4 cut(s) 57, 814, 1149, 1158
FaqI GGGAC 2 cut(s) 556, 945
FatI CATG 4 cut(s) 53, 810, 1145, 1154
FauNDI CATATG 1 cut(s) 1312
FbaI TGATCA 1 cut(s) 1231
FblI GTMKAC 1 cut(s) 637
Fnu4HI GCNGC 4 cut(s) 59, 270, 602, 693
FokI GGATG 6 cut(s) 75, 266, 272, 468, 645, 919
FriOI GRGCYC 1 cut(s) 1276
Fsp4HI GCNGC 4 cut(s) 59, 270, 602, 693
FspBI CTAG 5 cut(s) 273, 641, 696, 875, 1049
GluI GCNGC 4 cut(s) 59, 270, 602, 693
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 5 cut(s) 52, 238, 277, 604, 973
HapII CCGG 2 cut(s) 804, 1173
Hin1II CATG 4 cut(s) 57, 814, 1149, 1158
HincII GTYRAC 2 cut(s) 151, 477
HindII GTYRAC 2 cut(s) 151, 477
HinfI GANTC 6 cut(s) 64, 339, 489, 569, 987, 1184
HpaII CCGG 2 cut(s) 804, 1173
HphI GGTGA 5 cut(s) 40, 137, 293, 818, 839
Hpy166II GTNNAC 5 cut(s) 17, 151, 477, 638, 952
Hpy188I TCNGA 3 cut(s) 667, 994, 1278
Hpy188III TCNNGA 6 cut(s) 92, 118, 379, 520, 875, 1235
Hpy8I GTNNAC 5 cut(s) 17, 151, 477, 638, 952
HpyAV CCTTC 5 cut(s) 25, 419, 705, 779, 1154
HpyCH4III ACNGT 3 cut(s) 220, 731, 1060
HpyCH4IV ACGT 2 cut(s) 948, 1296
HpyF10VI GCNNNNNNNGC 9 cut(s) 58, 67, 189, 407, 601, 689, 1061, 1155, 1208
HpyF3I CTNAG 7 cut(s) 66, 93, 230, 746, 797, 991, 1356
HpySE526I ACGT 2 cut(s) 948, 1296
Hsp92II CATG 4 cut(s) 57, 814, 1149, 1158
Ksp22I TGATCA 1 cut(s) 1231
Kzo9I GATC 5 cut(s) 240, 283, 837, 957, 1231
LguI GCTCTTC 1 cut(s) 1298
LmnI GCTCC 4 cut(s) 10, 788, 851, 1271
Lsp1109I GCAGC 3 cut(s) 70, 281, 679
LweI GCATC 4 cut(s) 97, 604, 897, 1042
MaeI CTAG 5 cut(s) 273, 641, 696, 875, 1049
MaeII ACGT 2 cut(s) 948, 1296
MaeIII GTNAC 3 cut(s) 731, 806, 1112
MalI GATC 5 cut(s) 242, 285, 839, 959, 1233
MbiI CCGCTC 1 cut(s) 1032
MboI GATC 5 cut(s) 240, 283, 837, 957, 1231
MflI RGATCY 1 cut(s) 837
MhlI GDGCHC 2 cut(s) 1276, 1310
MluCI AATT 6 cut(s) 450, 548, 678, 1106, 1329, 1339
MlyI GAGTC 2 cut(s) 58, 578
MmeI TCCRAC 1 cut(s) 1289
Mph1103I ATGCAT 1 cut(s) 816
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 2 cut(s) 667, 860
MspA1I CMGCKG 1 cut(s) 601
MspI CCGG 2 cut(s) 804, 1173
MspR9I CCNGG 6 cut(s) 279, 324, 456, 471, 486, 708
MvaI CCWGG 6 cut(s) 279, 324, 456, 471, 486, 708
MwoI GCNNNNNNNGC 9 cut(s) 58, 67, 189, 407, 601, 689, 1061, 1155, 1208
NcoI CCATGG 1 cut(s) 53
NdeI CATATG 1 cut(s) 1312
NdeII GATC 5 cut(s) 240, 283, 837, 957, 1231
NlaIII CATG 4 cut(s) 57, 814, 1149, 1158
NlaIV GGNNCC 5 cut(s) 6, 315, 853, 933, 981
NmuCI GTSAC 2 cut(s) 731, 806
NsiI ATGCAT 1 cut(s) 816
NspI RCATGY 1 cut(s) 814
PciSI GCTCTTC 1 cut(s) 1298
PfeI GAWTC 4 cut(s) 339, 489, 987, 1184
PfoI TCCNGGA 3 cut(s) 322, 454, 484
PinAI ACCGGT 1 cut(s) 803
PkrI GCNGC 4 cut(s) 60, 271, 603, 694
PleI GAGTC 2 cut(s) 58, 577
PpsI GAGTC 2 cut(s) 58, 577
Ppu21I YACGTR 1 cut(s) 949
PpuMI RGGWCCY 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 1276
Psp5II RGGWCCY 1 cut(s) 314
Psp6I CCWGG 6 cut(s) 277, 322, 454, 469, 484, 706
PspGI CCWGG 6 cut(s) 277, 322, 454, 469, 484, 706
PspN4I GGNNCC 5 cut(s) 6, 315, 853, 933, 981
PspPI GGNCC 4 cut(s) 236, 314, 932, 971
PspPPI RGGWCCY 1 cut(s) 314
PstNI CAGNNNCTG 1 cut(s) 73
PsuI RGATCY 1 cut(s) 837
RsaI GTAC 3 cut(s) 419, 462, 917
RsaNI GTAC 3 cut(s) 418, 461, 916
RseI CAYNNNNRTG 2 cut(s) 667, 860
SacI GAGCTC 1 cut(s) 1276
SapI GCTCTTC 1 cut(s) 1298
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 4 cut(s) 59, 270, 602, 693
Sau3AI GATC 5 cut(s) 240, 283, 837, 957, 1231
Sau96I GGNCC 4 cut(s) 236, 314, 932, 971
SchI GAGTC 2 cut(s) 58, 578
ScrFI CCNGG 6 cut(s) 279, 324, 456, 471, 486, 708
SduI GDGCHC 2 cut(s) 1276, 1310
SfaNI GCATC 4 cut(s) 97, 604, 897, 1042
SfcI CTRYAG 1 cut(s) 1089
SgrAI CRCCGGYG 1 cut(s) 803
SinI GGWCC 2 cut(s) 314, 932
SmiMI CAYNNNNRTG 2 cut(s) 667, 860
SmlI CTYRAG 2 cut(s) 520, 881
SmoI CTYRAG 2 cut(s) 520, 881
Sse9I AATT 6 cut(s) 450, 548, 678, 1106, 1329, 1339
SsiI CCGC 3 cut(s) 369, 601, 1032
SspI AATATT 1 cut(s) 649
SspMI CTAG 5 cut(s) 273, 641, 696, 875, 1049
SstI GAGCTC 1 cut(s) 1276
StyD4I CCNGG 6 cut(s) 277, 322, 454, 469, 484, 706
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 3 cut(s) 220, 731, 1060
TaiI ACGT 2 cut(s) 951, 1299
TaqI TCGA 3 cut(s) 1008, 1023, 1182
TasI AATT 6 cut(s) 450, 548, 678, 1106, 1329, 1339
TauI GCSGC 1 cut(s) 604
TfiI GAWTC 4 cut(s) 339, 489, 987, 1184
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 8 cut(s) 78, 349, 505, 757, 780, 981, 1024, 1225
TseFI GTSAC 2 cut(s) 731, 806
TseI GCWGC 3 cut(s) 58, 269, 692
Tsp45I GTSAC 2 cut(s) 731, 806
TspDTI ATGAA 5 cut(s) 212, 339, 345, 549, 1316
TspRI CASTG 8 cut(s) 78, 349, 505, 757, 780, 981, 1024, 1225
VpaK11BI GGWCC 2 cut(s) 314, 932
XagI CCTNNNNNAGG 1 cut(s) 321
XapI RAATTY 3 cut(s) 450, 1106, 1339
XbaI TCTAGA 1 cut(s) 874
XceI RCATGY 1 cut(s) 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 330
XmiI GTMKAC 1 cut(s) 637
XspI CTAG 5 cut(s) 273, 641, 696, 875, 1049
Zsp2I ATGCAT 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.