AT5G04010

F-box protein At5g04010

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010949
Physical Location & Seq
Reverse (-)
1080105 .. 1081096
992 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04010.1

Sequence Viewer

Length: 864 bp
ATGTCCACATACGTGTCCTCTGTCTTCTCTCTCGCCACCATGAAACAAAACCCTCTGAAGCGCAAACGCAACGATGAGATCACTGAAGAGAAAGTCGTACTACTCATGGCTATGCACAAAGAAGACGACGAGCACCAACATACCAAACCGTCTCCACCATCTTGGGAGATTCTCTGCCTTGTTGGTCCTTACATGGACCCTGAGTCACTAGCCGTAGCCTCTTGCGTCTCCACCACGTGGTCGAAGTGCTTCTCCTCGGAAGATCTATGGAAGTCTCTACCAGCCACACGACATTCTATATTCGCTAAAGCCATCACCAAGGAGGGAGCTAAGCCCGCCTGGTTATCGTACAAGCGTCTAATCTCTGAAGCAGAATCTGCAGCAAAACGCCGCCGCAACAACCAACCCGCGGAGCCAAAGATCTCTCTCTCCGATCTTGTTTTCATCGTCCACGTGTCAGCTGGATCAAAAGAAGCAGTGGTTGTGAAACAGGGGAAGGATCTTGTGTTTGGCTCGAACGAGCGGTTCCAAATCGAAGCTGATGTCCGCGACTCGGGATTCACCGCCGATATGAAAGACGTGAGCATGTCGTGGAACGTCGTGTTAAGGAATTACGAAAGAATGTTTCTAATGTCGGAGACTGTTATAAAGTCATTAGATTCTATGATTGGATGGTTCACCGATGAACTTCCGGGGACAAAGAACCGTTACTGTGACGGTAGCAACCTGGTGGGAGAAGTCAAGCCAAGTTTTAATGAAGATGTTCTTGATAAAGTCGTGTTCGCTATTGCAGATTCACGCAACTGGAAGTCTCTGTTCGTTGACGATGTCTTGAGATATCTCCAATGTTTTCTTGTCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

287

Amino Acids

32.49

Weight (kDa)

6.03

Isoelectric Point (pI)

53.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 647
AccB7I CCANNNNNTGG 1 cut(s) 237
AccBSI CCGCTC 1 cut(s) 523
AccI GTMKAC 1 cut(s) 858
AccII CGCG 2 cut(s) 410, 549
AciI CCGC 8 cut(s) 336, 391, 394, 408, 410, 523, 547, 564
AclWI GGATC 2 cut(s) 472, 507
AcuI CTGAAG 3 cut(s) 77, 105, 387
AcvI CACGTG 2 cut(s) 237, 454
AdeI CACNNNGTG 1 cut(s) 237
AfaI GTAC 2 cut(s) 99, 350
AfiI CCNNNNNNNGG 3 cut(s) 237, 409, 553
AflIII ACRYGT 2 cut(s) 12, 453
AhdI GACNNNNNGTC 1 cut(s) 202
AjiI CACGTC 1 cut(s) 580
AjnI CCWGG 2 cut(s) 338, 726
AleI CACNNNNGTG 1 cut(s) 11
AluBI AGCT 3 cut(s) 329, 461, 539
AluI AGCT 3 cut(s) 329, 461, 539
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 5 cut(s) 156, 232, 279, 632, 816
AlwI GGATC 2 cut(s) 472, 507
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 553
ApeKI GCWGC 1 cut(s) 380
ArsI GACNNNNNNTTYG 2 cut(s) 528, 560
Asp700I GAANNNNTTC 2 cut(s) 248, 762
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 2 cut(s) 185, 196
AsuC2I CCSGG 1 cut(s) 693
AsuHPI GGTGA 3 cut(s) 307, 553, 670
AvaI CYCGRG 1 cut(s) 553
AvaII GGWCC 2 cut(s) 185, 196
BbrPI CACGTG 2 cut(s) 237, 454
BbsI GAAGAC 2 cut(s) 16, 129
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 392
BccI CCATC 3 cut(s) 166, 320, 666
BceAI ACGGC 1 cut(s) 197
BciT130I CCWGG 2 cut(s) 340, 728
BcnI CCSGG 1 cut(s) 693
BcoDI GTCTC 5 cut(s) 156, 232, 279, 632, 816
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 378
BglII AGATCT 2 cut(s) 262, 420
BisI GCNGC 3 cut(s) 381, 391, 394
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 3 cut(s) 382, 392, 395
Bme1390I CCNGG 3 cut(s) 340, 693, 728
Bme18I GGWCC 2 cut(s) 185, 196
BmeRI GACNNNNNGTC 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 553
BmgBI CACGTC 1 cut(s) 580
BmgT120I GGNCC 2 cut(s) 185, 196
BmiI GGNNCC 3 cut(s) 198, 414, 527
BmrFI CCNGG 3 cut(s) 340, 693, 728
BpiI GAAGAC 2 cut(s) 16, 129
Bpu1102I GCTNAGC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 853
BpuMI CCSGG 1 cut(s) 693
BsaAI YACGTR 3 cut(s) 13, 237, 454
BsaJI CCNNGG 4 cut(s) 255, 318, 408, 692
Bsc4I CCNNNNNNNGG 3 cut(s) 237, 409, 553
Bse1I ACTGG 1 cut(s) 809
BseBI CCWGG 2 cut(s) 340, 728
BseDI CCNNGG 4 cut(s) 255, 318, 408, 692
BseGI GGATG 1 cut(s) 677
BseLI CCNNNNNNNGG 3 cut(s) 237, 409, 553
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 1 cut(s) 809
BseRI GAGGAG 1 cut(s) 244
BseXI GCAGC 1 cut(s) 392
Bsh1236I CGCG 2 cut(s) 410, 549
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 1 cut(s) 692
BslFI GGGAC 1 cut(s) 709
BslI CCNNNNNNNGG 3 cut(s) 237, 409, 553
BsmAI GTCTC 5 cut(s) 156, 232, 279, 632, 816
BsmBI CGTCTC 2 cut(s) 156, 232
BsmFI GGGAC 1 cut(s) 709
BsoBI CYCGRG 1 cut(s) 553
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 6 cut(s) 78, 262, 420, 433, 464, 499
Bsp1720I GCTNAGC 1 cut(s) 330
BspACI CCGC 8 cut(s) 336, 391, 394, 408, 410, 523, 547, 564
BspCNI CTCAG 1 cut(s) 193
BspFNI CGCG 2 cut(s) 410, 549
BspLI GGNNCC 3 cut(s) 198, 414, 527
BspMAI CTGCAG 1 cut(s) 382
BspPI GGATC 2 cut(s) 472, 507
BsrBI CCGCTC 1 cut(s) 523
BsrI ACTGG 1 cut(s) 809
BssECI CCNNGG 4 cut(s) 255, 318, 408, 692
BssMI GATC 6 cut(s) 78, 262, 420, 433, 464, 499
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 2 cut(s) 340, 728
Bst4CI ACNGT 5 cut(s) 150, 643, 707, 713, 719
Bst6I CTCTTC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 377
BstBAI YACGTR 3 cut(s) 13, 237, 454
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 2 cut(s) 201, 330
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 677
BstFNI CGCG 2 cut(s) 410, 549
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 6 cut(s) 81, 265, 423, 436, 467, 502
BstMAI GTCTC 5 cut(s) 156, 232, 279, 632, 816
BstMBI GATC 6 cut(s) 78, 262, 420, 433, 464, 499
BstMWI GCNNNNNNNGC 2 cut(s) 335, 377
BstNI CCWGG 2 cut(s) 340, 728
BstNSI RCATGY 1 cut(s) 589
BstSCI CCNGG 3 cut(s) 338, 691, 726
BstSFI CTRYAG 1 cut(s) 378
BstUI CGCG 2 cut(s) 410, 549
BstV1I GCAGC 1 cut(s) 392
BstV2I GAAGAC 2 cut(s) 16, 129
BstX2I RGATCY 3 cut(s) 262, 420, 499
BstXI CCANNNNNNTGG 1 cut(s) 162
BstYI RGATCY 3 cut(s) 262, 420, 499
BtgI CCRYGG 1 cut(s) 408
BtrI CACGTC 1 cut(s) 580
BtsCI GGATG 1 cut(s) 677
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 2 cut(s) 81, 483
Cac8I GCNNGC 1 cut(s) 336
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 2 cut(s) 185, 196
Cfr42I CCGCGG 1 cut(s) 411
CseI GACGC 2 cut(s) 214, 344
CsiI ACCWGGT 1 cut(s) 726
Csp6I GTAC 2 cut(s) 98, 349
CviAII CATG 4 cut(s) 40, 106, 193, 586
CviQI GTAC 2 cut(s) 98, 349
DdeI CTNAG 2 cut(s) 201, 330
DpnI GATC 6 cut(s) 80, 264, 422, 435, 466, 501
DpnII GATC 6 cut(s) 78, 262, 420, 433, 464, 499
DraIII CACNNNGTG 1 cut(s) 237
DriI GACNNNNNGTC 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 81
Eam1105I GACNNNNNGTC 1 cut(s) 202
EarI CTCTTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 318
Eco32I GATATC 1 cut(s) 839
Eco47I GGWCC 2 cut(s) 185, 196
Eco57I CTGAAG 3 cut(s) 77, 105, 387
Eco72I CACGTG 2 cut(s) 237, 454
Eco88I CYCGRG 1 cut(s) 553
EcoRII CCWGG 2 cut(s) 338, 726
EcoRV GATATC 1 cut(s) 839
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
Esp3I CGTCTC 2 cut(s) 156, 232
FaeI CATG 4 cut(s) 43, 109, 196, 589
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FaqI GGGAC 1 cut(s) 709
FatI CATG 4 cut(s) 39, 105, 192, 585
FauI CCCGC 2 cut(s) 343, 415
FblI GTMKAC 1 cut(s) 858
Fnu4HI GCNGC 3 cut(s) 381, 391, 394
FokI GGATG 1 cut(s) 684
Fsp4HI GCNGC 3 cut(s) 381, 391, 394
FspBI CTAG 1 cut(s) 209
GlaI GCGC 1 cut(s) 62
GluI GCNGC 3 cut(s) 381, 391, 394
HapII CCGG 1 cut(s) 692
HgaI GACGC 2 cut(s) 214, 344
HhaI GCGC 1 cut(s) 63
Hin1II CATG 4 cut(s) 43, 109, 196, 589
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 2 cut(s) 823, 859
HindII GTYRAC 2 cut(s) 823, 859
HinfI GANTC 7 cut(s) 169, 203, 374, 551, 558, 659, 794
HpaII CCGG 1 cut(s) 692
HphI GGTGA 3 cut(s) 307, 553, 670
Hpy166II GTNNAC 5 cut(s) 6, 451, 678, 823, 859
Hpy188I TCNGA 5 cut(s) 57, 259, 367, 433, 637
Hpy188III TCNNGA 3 cut(s) 555, 767, 832
Hpy8I GTNNAC 5 cut(s) 6, 451, 678, 823, 859
Hpy99I CGWCG 2 cut(s) 131, 602
HpyAV CCTTC 1 cut(s) 490
HpyCH4III ACNGT 5 cut(s) 150, 643, 707, 713, 719
HpyCH4IV ACGT 5 cut(s) 12, 236, 453, 579, 597
HpyCH4V TGCA 3 cut(s) 115, 380, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 377
HpyF3I CTNAG 2 cut(s) 201, 330
HpySE526I ACGT 5 cut(s) 12, 236, 453, 579, 597
Hsp92II CATG 4 cut(s) 43, 109, 196, 589
HspAI GCGC 1 cut(s) 61
KspI CCGCGG 1 cut(s) 411
Kzo9I GATC 6 cut(s) 78, 262, 420, 433, 464, 499
LmnI GCTCC 2 cut(s) 326, 412
Lsp1109I GCAGC 1 cut(s) 392
MabI ACCWGGT 1 cut(s) 726
MaeI CTAG 1 cut(s) 209
MaeII ACGT 5 cut(s) 12, 236, 453, 579, 597
MaeIII GTNAC 3 cut(s) 204, 707, 713
MalI GATC 6 cut(s) 80, 264, 422, 435, 466, 501
MbiI CCGCTC 1 cut(s) 523
MboI GATC 6 cut(s) 78, 262, 420, 433, 464, 499
MboII GAAGA 5 cut(s) 16, 98, 134, 272, 770
MflI RGATCY 3 cut(s) 262, 420, 499
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 1 cut(s) 610
MlyI GAGTC 2 cut(s) 212, 545
MmeI TCCRAC 1 cut(s) 615
MnlI CCTC 5 cut(s) 28, 63, 229, 265, 316
MroXI GAANNNNTTC 2 cut(s) 248, 762
MseI TTAA 2 cut(s) 605, 753
MslI CAYNNNNRTG 2 cut(s) 11, 110
MspA1I CMGCKG 2 cut(s) 410, 461
MspI CCGG 1 cut(s) 692
MspR9I CCNGG 3 cut(s) 340, 693, 728
MvaI CCWGG 2 cut(s) 340, 728
MvnI CGCG 2 cut(s) 410, 549
MwoI GCNNNNNNNGC 2 cut(s) 335, 377
NciI CCSGG 1 cut(s) 693
NdeII GATC 6 cut(s) 78, 262, 420, 433, 464, 499
NlaIII CATG 4 cut(s) 43, 109, 196, 589
NlaIV GGNNCC 3 cut(s) 198, 414, 527
NmuCI GTSAC 2 cut(s) 204, 713
NspI RCATGY 1 cut(s) 589
OliI CACNNNNGTG 1 cut(s) 11
PdmI GAANNNNTTC 2 cut(s) 248, 762
PfeI GAWTC 5 cut(s) 169, 374, 558, 659, 794
PflFI GACNNNGTC 1 cut(s) 827
PflMI CCANNNNNTGG 1 cut(s) 237
PkrI GCNGC 3 cut(s) 382, 392, 395
PleI GAGTC 2 cut(s) 211, 545
PmaCI CACGTG 2 cut(s) 237, 454
PmlI CACGTG 2 cut(s) 237, 454
PpsI GAGTC 2 cut(s) 211, 545
Ppu21I YACGTR 3 cut(s) 13, 237, 454
PsiI TTATAA 1 cut(s) 647
Psp6I CCWGG 2 cut(s) 338, 726
PspCI CACGTG 2 cut(s) 237, 454
PspGI CCWGG 2 cut(s) 338, 726
PspN4I GGNNCC 3 cut(s) 198, 414, 527
PspPI GGNCC 2 cut(s) 185, 196
PstI CTGCAG 1 cut(s) 382
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 3 cut(s) 262, 420, 499
PsyI GACNNNGTC 1 cut(s) 827
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 2 cut(s) 99, 350
RsaNI GTAC 2 cut(s) 98, 349
RseI CAYNNNNRTG 2 cut(s) 11, 110
SacII CCGCGG 1 cut(s) 411
SalI GTCGAC 1 cut(s) 857
SaqAI TTAA 2 cut(s) 605, 753
SatI GCNGC 3 cut(s) 381, 391, 394
Sau3AI GATC 6 cut(s) 78, 262, 420, 433, 464, 499
Sau96I GGNCC 2 cut(s) 185, 196
SchI GAGTC 2 cut(s) 212, 545
ScrFI CCNGG 3 cut(s) 340, 693, 728
SduI GDGCHC 1 cut(s) 135
SetI ASST 9 cut(s) 15, 239, 331, 456, 463, 541, 582, 600, 729
SexAI ACCWGGT 1 cut(s) 726
SfcI CTRYAG 1 cut(s) 378
Sfr303I CCGCGG 1 cut(s) 411
SgrBI CCGCGG 1 cut(s) 411
SinI GGWCC 2 cut(s) 185, 196
SmiMI CAYNNNNRTG 2 cut(s) 11, 110
SmlI CTYRAG 1 cut(s) 832
SmoI CTYRAG 1 cut(s) 832
Sse9I AATT 1 cut(s) 610
SsiI CCGC 8 cut(s) 336, 391, 394, 408, 410, 523, 547, 564
SspMI CTAG 1 cut(s) 209
StyD4I CCNGG 3 cut(s) 338, 691, 726
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 5 cut(s) 150, 643, 707, 713, 719
TaiI ACGT 5 cut(s) 15, 239, 456, 582, 600
TaqI TCGA 4 cut(s) 242, 515, 534, 858
TasI AATT 1 cut(s) 610
TauI GCSGC 2 cut(s) 393, 396
TfiI GAWTC 5 cut(s) 169, 374, 558, 659, 794
Tru1I TTAA 2 cut(s) 605, 753
Tru9I TTAA 2 cut(s) 605, 753
TscAI CASTG 2 cut(s) 88, 483
TseFI GTSAC 2 cut(s) 204, 713
TseI GCWGC 1 cut(s) 380
Tsp45I GTSAC 2 cut(s) 204, 713
TspDTI ATGAA 5 cut(s) 56, 433, 587, 699, 771
TspRI CASTG 2 cut(s) 88, 483
Tth111I GACNNNGTC 1 cut(s) 827
Van91I CCANNNNNTGG 1 cut(s) 237
VpaK11BI GGWCC 2 cut(s) 185, 196
XceI RCATGY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 858
XmnI GAANNNNTTC 2 cut(s) 248, 762
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.