AT5G05010

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009135
Physical Location & Seq
Forward (+)
1476533 .. 1480197
3665 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05010.2

Sequence Viewer

Length: 1584 bp
ATGGTTGTGCTTGCTGCTGCCATTGTGGTCAAGTCTGGGAAAGTGATTGTTTCCAGACACTATGTGGATATGTCCCGTATCAGAATCGAAGGTCTACTTGCAGCTTTTCCTAAGCTGGTTGGAATGGGAAAGCAGCATACCTATATCGAGACGGAGAATGTGCGATATGTTTATCAGCCTATTGAAGCTCTGTTCCTGCTTCTTGTTACAACTAAGCAGAGTAACATTCTAGAAGACCTGGCCACCCTTACTTTGCTCTCCAAACTTGTACCTGAGTATTCCATGTCTTTAGATGAAGAAGGGATTTCCAGGGCATCTTTTGAGCTCATATTTGCATTTGATGAAGTCATATCTCTTGGGCACAAGGAAAGTGTGACAGTTGCCCAGGTGAAGCAGTATTGTGAAATGGAAAGTCATGAGGAGAAGTTGCATAAACTGGTTATGCAGAGCAAGATCAATGACACTAAGGACGTGATGAAGCGTAAGGCTAATGAGATTGACAAAAGCAAGATTGAAAAGAATAAGCCAGGAGGATTTTCGTCCATGGGTTCAATGGGATCTGGAAGACTTGAAAGTGGTTTCAATGAATTGAGCATATCCAGTGGTGGCGGCGGTGGTTATGGAAGTGGTTCTGGGTTTGGCATGATTTCAGATGTCGACCCTATCAATACCAAACCAAAAGATCGATCACGGTCTTCTGTTACTGCTCCTCCTAAGAGTTCTGGCATGAAACTTGGCAAGTCGGGAAAGAACCAGTTAATGGAGTCCCTTAAAGCAGAAGGTGAAGACGTCATTGAAGATGTTAAGCCTACTGGCCAATCCAAAGCAGCTGCCCCACCTCCAACTGATCCTTTCACATTAACTGTTGAAGAGAAGCTCAATGTTGCATTGAGACGAGATGGTGGACTTAGTAGCTTTGATATGCAGGGAACCCTGTCACTTCAAATTCTTAACCAAGAAGATGGATTTGTCCAAGTCCAGATTGCAACCGGTGAAAATCCCGAAATTCTTTTCAAGACCCATCCCAACATCAACCGAGATATGTTCAATAATGAGAATATTCTAGGGCTGAAGAGACCTGATCAGCCATTTCCCACTGGTCAAGGTGGAGATGGTGTTGGTCTTCTCAGGTGGAGAATGCAAAGAGCAGACGAGTCTATGGTGCCACTAACAATAAACTGCTGGCCTTCAGTCTCTGGAAACGAGACATATGTCAGCCTCGAGTACGAAGCCTCGTCCATGTTTGATCTGACTAATGTCATCATCTCCGTACCACTTCCTGCTCTGAGAGAGGCACCAAGCGTTAGACAATGCGATGGGGAGTGGAGGTACGACCCAAGAAATTCTGTTCTGGAATGGTCTATACTACTTATTGACAACTCCAACCGCAGTGGCTCAATGGAGTTTGTTGTGCCTCCAGTGGATTCATCGGTGTTCTTCCCCATCTCTGTTCAGTTTGCAGCGACAAGTACCTACAGTGGCTTGAAGGTGACTGGAATGATTCCTCTGAGAGGAGGTGGTGGTGCGACTCCTAGGTTTGTGCAGAGGACGCAGCTGATTGCCCAGAACTATCAAGTCATATGA

Protein Analysis

527

Amino Acids

57.72

Weight (kDa)

5.63

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 279 - 526 9.5e-42 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 792
AccB1I GGYRCC 2 cut(s) 1162, 1294
AccB7I CCANNNNNTGG 1 cut(s) 760
AccI GTMKAC 2 cut(s) 94, 657
AciI CCGC 3 cut(s) 609, 612, 1387
AclWI GGATC 2 cut(s) 565, 842
AcoI YGGCCR 2 cut(s) 240, 814
AcsI RAATTY 3 cut(s) 945, 1005, 1342
AcuI CTGAAG 2 cut(s) 1091, 1173
AcyI GRCGYC 1 cut(s) 789
AdeI CACNNNGTG 1 cut(s) 64
AfaI GTAC 5 cut(s) 270, 1226, 1272, 1331, 1471
AfiI CCNNNNNNNGG 2 cut(s) 760, 1511
AgeI ACCGGT 1 cut(s) 989
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 4 cut(s) 237, 308, 384, 526
AluBI AGCT 8 cut(s) 104, 115, 188, 325, 830, 877, 915, 1555
AluI AGCT 8 cut(s) 104, 115, 188, 325, 830, 877, 915, 1555
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 5 cut(s) 143, 886, 1069, 1198, 1199
AlwI GGATC 2 cut(s) 565, 842
AlwNI CAGNNNCTG 1 cut(s) 1196
Ama87I CYCGRG 1 cut(s) 1220
AoxI GGCC 3 cut(s) 240, 814, 1184
ApeKI GCWGC 8 cut(s) 14, 17, 101, 133, 827, 830, 1460, 1552
ApoI RAATTY 3 cut(s) 945, 1005, 1342
AsiGI ACCGGT 1 cut(s) 989
Asp700I GAANNNNTTC 1 cut(s) 628
AspA2I CCTAGG 1 cut(s) 1532
AsuHPI GGTGA 4 cut(s) 400, 794, 1004, 1501
AvaI CYCGRG 1 cut(s) 1220
AvrII CCTAGG 1 cut(s) 1532
BaeGI GKGCMC 1 cut(s) 363
BalI TGGCCA 2 cut(s) 242, 816
BanI GGYRCC 2 cut(s) 1162, 1294
BanII GRGCYC 1 cut(s) 327
BbsI GAAGAC 5 cut(s) 240, 571, 687, 792, 1115
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 7 cut(s) 4, 113, 145, 817, 839, 1472, 1564
BccI CCATC 6 cut(s) 893, 956, 1029, 1106, 1310, 1451
BciT130I CCWGG 4 cut(s) 239, 310, 386, 528
BclI TGATCA 1 cut(s) 1081
BcoDI GTCTC 5 cut(s) 143, 886, 1069, 1198, 1199
BfaI CTAG 3 cut(s) 230, 1064, 1533
BfmI CTRYAG 1 cut(s) 1474
BisI GCNGC 9 cut(s) 15, 18, 102, 134, 610, 828, 831, 1461, 1553
BlnI CCTAGG 1 cut(s) 1532
BlsI GCNGC 9 cut(s) 16, 19, 103, 135, 611, 829, 832, 1462, 1554
Bme1390I CCNGG 4 cut(s) 239, 310, 386, 528
BmeT110I CYCGRG 1 cut(s) 1220
BmgBI CACGTC 1 cut(s) 472
BmiI GGNNCC 3 cut(s) 931, 1164, 1296
BmrFI CCNGG 4 cut(s) 239, 310, 386, 528
BmsI GCATC 1 cut(s) 323
BoxI GACNNNNGTC 2 cut(s) 1211, 1256
BpiI GAAGAC 5 cut(s) 240, 571, 687, 792, 1115
BpmI CTGGAG 1 cut(s) 1401
Bpu10I CCTNAGC 1 cut(s) 111
Bsa29I ATCGAT 1 cut(s) 685
BsaHI GRCGYC 1 cut(s) 789
BsaI GGTCTC 1 cut(s) 1069
BsaJI CCNNGG 4 cut(s) 309, 384, 543, 1532
BsaWI WCCGGW 1 cut(s) 989
BsaXI ACNNNNNCTCC 6 cut(s) 694, 724, 1101, 1131, 1313, 1343
Bsc4I CCNNNNNNNGG 2 cut(s) 760, 1511
Bse118I RCCGGY 1 cut(s) 989
Bse1I ACTGG 7 cut(s) 441, 600, 754, 817, 1102, 1418, 1498
BseBI CCWGG 4 cut(s) 239, 310, 386, 528
BseCI ATCGAT 1 cut(s) 685
BseDI CCNNGG 4 cut(s) 309, 384, 543, 1532
BseGI GGATG 1 cut(s) 1021
BseLI CCNNNNNNNGG 2 cut(s) 760, 1511
BseMII CTCAG 4 cut(s) 264, 1141, 1277, 1499
BseNI ACTGG 7 cut(s) 441, 600, 754, 817, 1102, 1418, 1498
BseRI GAGGAG 3 cut(s) 434, 699, 1527
BseSI GKGCMC 1 cut(s) 363
BseXI GCAGC 7 cut(s) 4, 113, 145, 817, 839, 1472, 1564
BsgI GTGCAG 1 cut(s) 1562
BshFI GGCC 3 cut(s) 242, 816, 1186
BshNI GGYRCC 2 cut(s) 1162, 1294
BshTI ACCGGT 1 cut(s) 989
BshVI ATCGAT 1 cut(s) 685
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 1220
BsiSI CCGG 1 cut(s) 990
BslFI GGGAC 2 cut(s) 58, 751
BslI CCNNNNNNNGG 2 cut(s) 760, 1511
BsmAI GTCTC 5 cut(s) 143, 886, 1069, 1198, 1199
BsmBI CGTCTC 2 cut(s) 143, 886
BsmFI GGGAC 2 cut(s) 58, 751
BsmI GAATGC 1 cut(s) 1143
BsnI GGCC 3 cut(s) 242, 816, 1186
Bso31I GGTCTC 1 cut(s) 1069
BsoBI CYCGRG 1 cut(s) 1220
Bsp1286I GDGCHC 2 cut(s) 327, 363
Bsp143I GATC 7 cut(s) 453, 557, 682, 686, 847, 1081, 1246
Bsp19I CCATGG 1 cut(s) 543
BspACI CCGC 3 cut(s) 609, 612, 1387
BspANI GGCC 3 cut(s) 242, 816, 1186
BspCNI CTCAG 4 cut(s) 265, 1140, 1278, 1500
BspDI ATCGAT 1 cut(s) 685
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 931, 1164, 1296
BspPI GGATC 2 cut(s) 565, 842
BspT107I GGYRCC 2 cut(s) 1162, 1294
BspTNI GGTCTC 1 cut(s) 1069
BsrFI RCCGGY 1 cut(s) 989
BsrI ACTGG 7 cut(s) 441, 600, 754, 817, 1102, 1418, 1498
BssAI RCCGGY 1 cut(s) 989
BssECI CCNNGG 4 cut(s) 309, 384, 543, 1532
BssMI GATC 7 cut(s) 453, 557, 682, 686, 847, 1081, 1246
BssNI GRCGYC 1 cut(s) 789
BssT1I CCWWGG 2 cut(s) 543, 1532
Bst2UI CCWGG 4 cut(s) 239, 310, 386, 528
Bst4CI ACNGT 4 cut(s) 379, 693, 865, 1478
Bst6I CTCTTC 2 cut(s) 864, 1067
BstACI GRCGYC 1 cut(s) 789
BstC8I GCNNGC 2 cut(s) 12, 1184
BstDEI CTNAG 9 cut(s) 111, 213, 273, 465, 714, 908, 1127, 1286, 1508
BstDSI CCRYGG 1 cut(s) 543
BstENI CCTNNNNNAGG 1 cut(s) 1509
BstF5I GGATG 1 cut(s) 1021
BstKTI GATC 7 cut(s) 456, 560, 685, 689, 850, 1084, 1249
BstMAI GTCTC 5 cut(s) 143, 886, 1069, 1198, 1199
BstMBI GATC 7 cut(s) 453, 557, 682, 686, 847, 1081, 1246
BstMWI GCNNNNNNNGC 1 cut(s) 1549
BstNI CCWGG 4 cut(s) 239, 310, 386, 528
BstPAI GACNNNNGTC 2 cut(s) 1211, 1256
BstSCI CCNGG 4 cut(s) 237, 308, 384, 526
BstSFI CTRYAG 1 cut(s) 1474
BstSLI GKGCMC 1 cut(s) 363
BstV1I GCAGC 7 cut(s) 4, 113, 145, 817, 839, 1472, 1564
BstV2I GAAGAC 5 cut(s) 240, 571, 687, 792, 1115
BstX2I RGATCY 1 cut(s) 557
BstXI CCANNNNNNTGG 1 cut(s) 962
BstYI RGATCY 1 cut(s) 557
Bsu15I ATCGAT 1 cut(s) 685
BsuRI GGCC 3 cut(s) 242, 816, 1186
BsuTUI ATCGAT 1 cut(s) 685
BtgI CCRYGG 1 cut(s) 543
BtgZI GCGATG 1 cut(s) 1329
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 1 cut(s) 1021
BtsI GCAGTG 1 cut(s) 1396
BtsIMutI CAGTG 5 cut(s) 607, 1095, 1396, 1425, 1483
Cac8I GCNNGC 2 cut(s) 12, 1184
CaiI CAGNNNCTG 1 cut(s) 1196
CciI TCATGA 1 cut(s) 415
Cfr10I RCCGGY 1 cut(s) 989
ClaI ATCGAT 1 cut(s) 685
CseI GACGC 1 cut(s) 1558
Csp6I GTAC 5 cut(s) 269, 1225, 1271, 1330, 1470
CspAI ACCGGT 1 cut(s) 989
CspCI CAANNNNNGTGG 2 cut(s) 1372, 1407
CviAII CATG 6 cut(s) 283, 416, 544, 643, 727, 1240
CviQI GTAC 5 cut(s) 269, 1225, 1271, 1330, 1470
DdeI CTNAG 9 cut(s) 111, 213, 273, 465, 714, 908, 1127, 1286, 1508
DpnI GATC 7 cut(s) 455, 559, 684, 688, 849, 1083, 1248
DpnII GATC 7 cut(s) 453, 557, 682, 686, 847, 1081, 1246
DraIII CACNNNGTG 1 cut(s) 64
EaeI YGGCCR 2 cut(s) 240, 814
Eam1104I CTCTTC 2 cut(s) 864, 1067
EarI CTCTTC 2 cut(s) 864, 1067
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 2 cut(s) 543, 1532
Eco24I GRGCYC 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 1069
Eco53kI GAGCTC 1 cut(s) 325
Eco57I CTGAAG 2 cut(s) 1091, 1173
Eco88I CYCGRG 1 cut(s) 1220
EcoICRI GAGCTC 1 cut(s) 325
EcoNI CCTNNNNNAGG 1 cut(s) 1509
EcoRII CCWGG 4 cut(s) 237, 308, 384, 526
EcoT14I CCWWGG 2 cut(s) 543, 1532
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 2 cut(s) 543, 1532
Esp3I CGTCTC 2 cut(s) 143, 886
FaeI CATG 6 cut(s) 286, 419, 547, 646, 730, 1243
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FaqI GGGAC 2 cut(s) 58, 751
FatI CATG 6 cut(s) 282, 415, 543, 642, 726, 1239
FauNDI CATATG 2 cut(s) 1210, 1580
FbaI TGATCA 1 cut(s) 1081
FblI GTMKAC 2 cut(s) 94, 657
Fnu4HI GCNGC 9 cut(s) 15, 18, 102, 134, 610, 828, 831, 1461, 1553
FokI GGATG 1 cut(s) 1008
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 9 cut(s) 15, 18, 102, 134, 610, 828, 831, 1461, 1553
FspBI CTAG 3 cut(s) 230, 1064, 1533
GluI GCNGC 9 cut(s) 15, 18, 102, 134, 610, 828, 831, 1461, 1553
GsuI CTGGAG 1 cut(s) 1401
HaeIII GGCC 3 cut(s) 242, 816, 1186
HapII CCGG 1 cut(s) 990
HgaI GACGC 1 cut(s) 1558
Hin1I GRCGYC 1 cut(s) 789
Hin1II CATG 6 cut(s) 286, 419, 547, 646, 730, 1243
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HinfI GANTC 6 cut(s) 84, 764, 1154, 1424, 1501, 1528
HpaII CCGG 1 cut(s) 990
HphI GGTGA 4 cut(s) 400, 794, 1004, 1501
Hpy166II GTNNAC 3 cut(s) 95, 658, 905
Hpy188I TCNGA 5 cut(s) 83, 652, 1251, 1287, 1509
Hpy8I GTNNAC 3 cut(s) 95, 658, 905
HpyAV CCTTC 5 cut(s) 83, 293, 773, 1197, 1480
HpyCH4III ACNGT 4 cut(s) 379, 693, 865, 1478
HpyCH4IV ACGT 2 cut(s) 471, 789
HpyF10VI GCNNNNNNNGC 1 cut(s) 1549
HpyF3I CTNAG 9 cut(s) 111, 213, 273, 465, 714, 908, 1127, 1286, 1508
HpySE526I ACGT 2 cut(s) 471, 789
Hsp92I GRCGYC 1 cut(s) 789
Hsp92II CATG 6 cut(s) 286, 419, 547, 646, 730, 1243
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 7 cut(s) 453, 557, 682, 686, 847, 1081, 1246
LmnI GCTCC 1 cut(s) 712
Lsp1109I GCAGC 7 cut(s) 4, 113, 145, 817, 839, 1472, 1564
LweI GCATC 1 cut(s) 323
MaeI CTAG 3 cut(s) 230, 1064, 1533
MaeII ACGT 2 cut(s) 471, 789
MaeIII GTNAC 6 cut(s) 205, 221, 373, 700, 936, 1489
MalI GATC 7 cut(s) 455, 559, 684, 688, 849, 1083, 1248
MboI GATC 7 cut(s) 453, 557, 682, 686, 847, 1081, 1246
MflI RGATCY 1 cut(s) 557
MhlI GDGCHC 2 cut(s) 327, 363
MlsI TGGCCA 2 cut(s) 242, 816
MluCI AATT 4 cut(s) 587, 945, 1005, 1342
MluNI TGGCCA 2 cut(s) 242, 816
MlyI GAGTC 3 cut(s) 773, 1163, 1522
MmeI TCCRAC 3 cut(s) 100, 866, 1407
Mox20I TGGCCA 2 cut(s) 242, 816
MroXI GAANNNNTTC 1 cut(s) 628
MscI TGGCCA 2 cut(s) 242, 816
MseI TTAA 5 cut(s) 758, 771, 804, 860, 951
Msp20I TGGCCA 2 cut(s) 242, 816
MspA1I CMGCKG 2 cut(s) 830, 1555
MspI CCGG 1 cut(s) 990
MspR9I CCNGG 4 cut(s) 239, 310, 386, 528
Mva1269I GAATGC 1 cut(s) 1143
MvaI CCWGG 4 cut(s) 239, 310, 386, 528
MwoI GCNNNNNNNGC 1 cut(s) 1549
NcoI CCATGG 1 cut(s) 543
NdeI CATATG 2 cut(s) 1210, 1580
NdeII GATC 7 cut(s) 453, 557, 682, 686, 847, 1081, 1246
NlaIII CATG 6 cut(s) 286, 419, 547, 646, 730, 1243
NlaIV GGNNCC 3 cut(s) 931, 1164, 1296
NmuCI GTSAC 3 cut(s) 373, 936, 1489
PaeR7I CTCGAG 1 cut(s) 1220
PagI TCATGA 1 cut(s) 415
PctI GAATGC 1 cut(s) 1143
PdmI GAANNNNTTC 1 cut(s) 628
PfeI GAWTC 3 cut(s) 84, 1424, 1501
PflMI CCANNNNNTGG 1 cut(s) 760
PinAI ACCGGT 1 cut(s) 989
PkrI GCNGC 9 cut(s) 16, 19, 103, 135, 611, 829, 832, 1462, 1554
PleI GAGTC 3 cut(s) 772, 1162, 1522
PpsI GAGTC 3 cut(s) 772, 1162, 1522
PshAI GACNNNNGTC 2 cut(s) 1211, 1256
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 4 cut(s) 237, 308, 384, 526
PspGI CCWGG 4 cut(s) 237, 308, 384, 526
PspN4I GGNNCC 3 cut(s) 931, 1164, 1296
PspXI VCTCGAGB 1 cut(s) 1220
PstNI CAGNNNCTG 1 cut(s) 1196
PsuI RGATCY 1 cut(s) 557
PvuII CAGCTG 2 cut(s) 830, 1555
RsaI GTAC 5 cut(s) 270, 1226, 1272, 1331, 1471
RsaNI GTAC 5 cut(s) 269, 1225, 1271, 1330, 1470
SacI GAGCTC 1 cut(s) 327
SalI GTCGAC 1 cut(s) 656
SaqAI TTAA 5 cut(s) 758, 771, 804, 860, 951
SatI GCNGC 9 cut(s) 15, 18, 102, 134, 610, 828, 831, 1461, 1553
Sau3AI GATC 7 cut(s) 453, 557, 682, 686, 847, 1081, 1246
SchI GAGTC 3 cut(s) 773, 1163, 1522
ScrFI CCNGG 4 cut(s) 239, 310, 386, 528
SduI GDGCHC 2 cut(s) 327, 363
SfaNI GCATC 1 cut(s) 323
SfcI CTRYAG 1 cut(s) 1474
Sfr274I CTCGAG 1 cut(s) 1220
SlaI CTCGAG 1 cut(s) 1220
SmlI CTYRAG 1 cut(s) 1220
SmoI CTYRAG 1 cut(s) 1220
Sse9I AATT 4 cut(s) 587, 945, 1005, 1342
SsiI CCGC 3 cut(s) 609, 612, 1387
SspI AATATT 1 cut(s) 1060
SspMI CTAG 3 cut(s) 230, 1064, 1533
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 4 cut(s) 237, 308, 384, 526
StyI CCWWGG 2 cut(s) 543, 1532
TaaI ACNGT 4 cut(s) 379, 693, 865, 1478
TaiI ACGT 2 cut(s) 474, 792
TaqI TCGA 5 cut(s) 87, 147, 657, 685, 1221
TasI AATT 4 cut(s) 587, 945, 1005, 1342
TauI GCSGC 1 cut(s) 612
TfiI GAWTC 3 cut(s) 84, 1424, 1501
Tru1I TTAA 5 cut(s) 758, 771, 804, 860, 951
Tru9I TTAA 5 cut(s) 758, 771, 804, 860, 951
TscAI CASTG 5 cut(s) 607, 1102, 1396, 1425, 1483
TseFI GTSAC 3 cut(s) 373, 936, 1489
TseI GCWGC 8 cut(s) 14, 17, 101, 133, 827, 830, 1460, 1552
Tsp45I GTSAC 3 cut(s) 373, 936, 1489
TspDTI ATGAA 6 cut(s) 309, 357, 491, 600, 743, 1416
TspGWI ACGGA 2 cut(s) 167, 1258
TspRI CASTG 5 cut(s) 607, 1102, 1396, 1425, 1483
Van91I CCANNNNNTGG 1 cut(s) 760
XagI CCTNNNNNAGG 1 cut(s) 1509
XapI RAATTY 3 cut(s) 945, 1005, 1342
XbaI TCTAGA 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 2 cut(s) 61, 550
XhoI CTCGAG 1 cut(s) 1220
XmaJI CCTAGG 1 cut(s) 1532
XmiI GTMKAC 2 cut(s) 94, 657
XmnI GAANNNNTTC 1 cut(s) 628
XspI CTAG 3 cut(s) 230, 1064, 1533
ZraI GACGTC 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.