Rh7DG298400

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009135
Physical Location & Seq
Reverse (-)
34005998 .. 34024773
18776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG298400.1

Sequence Viewer

Length: 1677 bp
ATGGAGGCAGCTTTGTATCAAGCGGAGGAAGCAAAAATAGCTATTGCAACTATTATTGAGTACATTGCAACTGAATCTGGTCACATATTTCAAGCAGTTTTCGTTGGAGCTGCAATTTTCCCGATAGTGCTTGTTTCGAGGCAGTTTATGGATATGTCTCGTATAAGGATTGAGGGTCTTCTTGCGGCTTTCCCTAAGTTGGTAGGCACTGGCAAACAACATACATATGTCGAGACGGAAAATGTGCGCTATGTTTACCAGCCAATAGAAGCTTTGTATTTGCTTCTAGTTACAAACAAGCAGAGCAATATCTTAGAAGATTTGGAGACTTTGAGGCTGCTCTCCAAGCTTGTACCTGAATATGCATTGTCCCTAGATGAAGAGGGTATATGCAGGACAGCTTTCGAGTTGATTTTTGCTTTTGATGAAGTAATCTCTCTTGGGCACAAGGAGAATGTGACAGTTGCACAGGTGAAGCAGTACTGTGAGATGGAGAGTCATGAAGAGAAGCTTCACAAGCTCGTAATGCAGAGCAAGATAAATGAGACCAAGGATGTTATGAAGCGTAAAGCCAGTGAAATTGACAAAAGCAAGATTGATAGGAGCCGAGGTGAAAAGGGAGGATTTGGGCCTTTGCAGTCGATGGGTTCTGGAAGAATTGAGAGTGCCTTTAGTGAGATGAGCTTATCCAGTACTGGAAGTGGTTTTGGAAGTGGTTCTGGTTTGGGAGCGACCACTGATTTTGAAACATTTTCTTCGAAGGCTAAAGGCCGTCCACCTTCATCTGCCACAGCTCCCCCAAAGGGTTTAGGCATGCAGCTTGGAAAATCACAAAGGACCAACCAATTTTTAGAGTCCTTAAAAGCGGAGGGAGAGGTGATTCTTGAAGATGTACAGCCAAAAGCAGGCCAGTCAAAATCAGTTGCCCCACCTTTAACTGATCCCGTCACTTTGAATGTCGAAGAGAAACTTAATGTAACACTTAAACGTGATGGTGGAGTCAGTAACTTTGATGTTCAAGGAACATTATCCTTGCAAATTCTTAACCAAGAAGACGGGCATATCCAAGTCCAGATTGAAACCGGTGGAAATCCTGGCATCCTTTTTAAAACTCACCCTAACATGAACAAAGAACTATTTTCTAATGAAAATATTCTAGGCTTGAAAGATCCTAATAGGCCTTTCCCCACTGGTCAAGCTGGTGATGCAGCAGGTGTTGGTCTTTTGAAATGGAGAATGCAGAGTCAAGATGAATCATTGGTGCCTCTACAAATCAACTGTTGGCCCTCTGTTTCTGGAAATGAAACCTATGTCAACATTGAGTATGATGCTTCATCAATGTTCGATCTGCGAAATGTTGTCATTTCAGTACCTCTTCCAGCTCTCAGAGAGGCTCCAGTTGTGAAGGAGATTCATGGTGAATGGAGATATGATGCAAGGAATTCAGTATTGGAGTGGTCTATACTTCTTATTGATAACTCAAACCGCAGTGGATCAATGGAGTTTGTTGTTCCTCCAGCAGACTCCAATGTATTCTTTCCCATTTCTGTGCGCTTCTCTGCCACTAGTACATTCAGTGACCTGAAGGTTGTGAATATCTTACCTCTGAAAGGTGGAGCCCCTCCCAAGTTCGCTCAGAGAACACAGTTGCTCACAGAGAACTACCAGGTGGTGTAA

Protein Analysis

558

Amino Acids

61.32

Weight (kDa)

5.33

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 313 - 542 2.4e-43 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1202
Acc36I ACCTGC 1 cut(s) 1202
AccB1I GGYRCC 1 cut(s) 1261
AciI CCGC 4 cut(s) 23, 185, 866, 1486
AclWI GGATC 3 cut(s) 935, 1163, 1501
AcsI RAATTY 2 cut(s) 1038, 1441
AcuI CTGAAG 1 cut(s) 1604
AfaI GTAC 7 cut(s) 62, 354, 482, 694, 894, 1371, 1570
AfiI CCNNNNNNNGG 4 cut(s) 199, 803, 905, 1610
AgeI ACCGGT 1 cut(s) 1082
AgsI TTSAA 8 cut(s) 92, 746, 887, 955, 1019, 1079, 1165, 1228
AhlI ACTAGT 1 cut(s) 1565
AjnI CCWGG 2 cut(s) 1093, 1665
AjuI GAANNNNNNNTTGG 2 cut(s) 1433, 1465
Alw26I GTCTC 4 cut(s) 162, 227, 320, 539
AlwI GGATC 3 cut(s) 935, 1163, 1501
AoxI GGCC 5 cut(s) 629, 769, 907, 1178, 1283
ApeKI GCWGC 5 cut(s) 8, 110, 337, 817, 1208
ApoI RAATTY 2 cut(s) 1038, 1441
AsiGI ACCGGT 1 cut(s) 1082
Asp700I GAANNNNTTC 2 cut(s) 715, 1152
AspLEI GCGC 2 cut(s) 249, 1554
AspS9I GGNCC 3 cut(s) 629, 837, 1284
AsuHPI GGTGA 6 cut(s) 484, 623, 889, 1106, 1214, 1430
AsuII TTCGAA 1 cut(s) 758
AvaII GGWCC 1 cut(s) 837
BaeGI GKGCMC 1 cut(s) 447
BanI GGYRCC 1 cut(s) 1261
BanII GRGCYC 1 cut(s) 1621
BbsI GAAGAC 2 cut(s) 170, 1059
BbvI GCAGC 5 cut(s) 20, 97, 324, 829, 1220
BccI CCATC 3 cut(s) 484, 637, 986
BceAI ACGGC 1 cut(s) 756
BciT130I CCWGG 2 cut(s) 1095, 1667
BcoDI GTCTC 4 cut(s) 162, 227, 320, 539
BcuI ACTAGT 1 cut(s) 1565
BfaI CTAG 4 cut(s) 287, 374, 1157, 1566
BfuAI ACCTGC 1 cut(s) 1202
BisI GCNGC 6 cut(s) 9, 111, 186, 338, 818, 1209
BlsI GCNGC 6 cut(s) 10, 112, 187, 339, 819, 1210
BmcAI AGTACT 2 cut(s) 482, 694
Bme1390I CCNGG 2 cut(s) 1095, 1667
Bme18I GGWCC 1 cut(s) 837
BmgT120I GGNCC 3 cut(s) 629, 837, 1284
BmiI GGNNCC 4 cut(s) 605, 1263, 1395, 1618
BmrFI CCNGG 2 cut(s) 1095, 1667
BmsI GCATC 4 cut(s) 1107, 1195, 1318, 1423
BpiI GAAGAC 2 cut(s) 170, 1059
BpmI CTGGAG 2 cut(s) 1380, 1500
Bpu14I TTCGAA 1 cut(s) 758
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 2 cut(s) 549, 607
BsaWI WCCGGW 1 cut(s) 1082
BsaXI ACNNNNNCTCC 2 cut(s) 595, 625
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 803, 905, 1610
Bse118I RCCGGY 1 cut(s) 1082
Bse1I ACTGG 7 cut(s) 214, 573, 690, 700, 910, 1195, 1397
Bse3DI GCAATG 1 cut(s) 63
BseBI CCWGG 2 cut(s) 1095, 1667
BseDI CCNNGG 2 cut(s) 549, 607
BseGI GGATG 2 cut(s) 559, 1098
BseLI CCNNNNNNNGG 4 cut(s) 199, 803, 905, 1610
BseMI GCAATG 1 cut(s) 63
BseMII CTCAG 2 cut(s) 1399, 1649
BseNI ACTGG 7 cut(s) 214, 573, 690, 700, 910, 1195, 1397
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 5 cut(s) 20, 97, 324, 829, 1220
BshFI GGCC 5 cut(s) 631, 771, 909, 1180, 1285
BshNI GGYRCC 1 cut(s) 1261
BshTI ACCGGT 1 cut(s) 1082
BsiSI CCGG 1 cut(s) 1083
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 4 cut(s) 199, 803, 905, 1610
BsmAI GTCTC 4 cut(s) 162, 227, 320, 539
BsmBI CGTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 355
BsmI GAATGC 1 cut(s) 1242
BsnI GGCC 5 cut(s) 631, 771, 909, 1180, 1285
Bso31I GGTCTC 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 758
Bsp1286I GDGCHC 2 cut(s) 447, 1621
Bsp1407I TGTACA 1 cut(s) 892
Bsp143I GATC 4 cut(s) 940, 1168, 1345, 1493
BspACI CCGC 4 cut(s) 23, 185, 866, 1486
BspANI GGCC 5 cut(s) 631, 771, 909, 1180, 1285
BspCNI CTCAG 2 cut(s) 1398, 1648
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 4 cut(s) 605, 1263, 1395, 1618
BspMI ACCTGC 1 cut(s) 1202
BspPI GGATC 3 cut(s) 935, 1163, 1501
BspT104I TTCGAA 1 cut(s) 758
BspT107I GGYRCC 1 cut(s) 1261
BspTNI GGTCTC 1 cut(s) 539
BsrDI GCAATG 1 cut(s) 63
BsrFI RCCGGY 1 cut(s) 1082
BsrGI TGTACA 1 cut(s) 892
BsrI ACTGG 7 cut(s) 214, 573, 690, 700, 910, 1195, 1397
BssAI RCCGGY 1 cut(s) 1082
BssECI CCNNGG 2 cut(s) 549, 607
BssMI GATC 4 cut(s) 940, 1168, 1345, 1493
BssT1I CCWWGG 1 cut(s) 549
Bst2UI CCWGG 2 cut(s) 1095, 1667
Bst4CI ACNGT 4 cut(s) 463, 485, 1280, 1647
Bst6I CTCTTC 4 cut(s) 375, 498, 957, 1380
BstAUI TGTACA 1 cut(s) 892
BstBI TTCGAA 1 cut(s) 758
BstC8I GCNNGC 2 cut(s) 815, 907
BstDEI CTNAG 4 cut(s) 195, 313, 1385, 1635
BstENI CCTNNNNNAGG 1 cut(s) 1608
BstF5I GGATG 2 cut(s) 559, 1098
BstHHI GCGC 2 cut(s) 249, 1554
BstKTI GATC 4 cut(s) 943, 1171, 1348, 1496
BstMAI GTCTC 4 cut(s) 162, 227, 320, 539
BstMBI GATC 4 cut(s) 940, 1168, 1345, 1493
BstMWI GCNNNNNNNGC 6 cut(s) 29, 38, 346, 517, 526, 1205
BstNI CCWGG 2 cut(s) 1095, 1667
BstNSI RCATGY 1 cut(s) 817
BstSCI CCNGG 2 cut(s) 1093, 1665
BstSLI GKGCMC 1 cut(s) 447
BstV1I GCAGC 5 cut(s) 20, 97, 324, 829, 1220
BstV2I GAAGAC 2 cut(s) 170, 1059
BstX2I RGATCY 1 cut(s) 1168
BstYI RGATCY 1 cut(s) 1168
BsuRI GGCC 5 cut(s) 631, 771, 909, 1180, 1285
BtsCI GGATG 2 cut(s) 559, 1098
BtsI GCAGTG 1 cut(s) 1495
BtsIMutI CAGTG 6 cut(s) 207, 580, 735, 1188, 1495, 1582
BveI ACCTGC 1 cut(s) 1202
Cac8I GCNNGC 2 cut(s) 815, 907
CciI TCATGA 1 cut(s) 499
CfoI GCGC 2 cut(s) 249, 1554
Cfr10I RCCGGY 1 cut(s) 1082
Cfr13I GGNCC 3 cut(s) 629, 837, 1284
CsiI ACCWGGT 1 cut(s) 1665
Csp6I GTAC 7 cut(s) 61, 353, 481, 693, 893, 1370, 1569
CspAI ACCGGT 1 cut(s) 1082
CspCI CAANNNNNGTGG 2 cut(s) 724, 759
CviAII CATG 4 cut(s) 500, 814, 1123, 1415
CviQI GTAC 7 cut(s) 61, 353, 481, 693, 893, 1370, 1569
DdeI CTNAG 4 cut(s) 195, 313, 1385, 1635
DpnI GATC 4 cut(s) 942, 1170, 1347, 1495
DpnII GATC 4 cut(s) 940, 1168, 1345, 1493
DraI TTTAAA 1 cut(s) 1108
Eam1104I CTCTTC 4 cut(s) 375, 498, 957, 1380
EarI CTCTTC 4 cut(s) 375, 498, 957, 1380
Eco130I CCWWGG 1 cut(s) 549
Eco147I AGGCCT 1 cut(s) 1180
Eco24I GRGCYC 1 cut(s) 1621
Eco31I GGTCTC 1 cut(s) 539
Eco47I GGWCC 1 cut(s) 837
Eco57I CTGAAG 1 cut(s) 1604
EcoNI CCTNNNNNAGG 1 cut(s) 1608
EcoRI GAATTC 1 cut(s) 1441
EcoRII CCWGG 2 cut(s) 1093, 1665
EcoT14I CCWWGG 1 cut(s) 549
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 1621
ErhI CCWWGG 1 cut(s) 549
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 4 cut(s) 503, 817, 1126, 1418
FalI AAGNNNNNCTT 4 cut(s) 495, 527, 954, 986
FaqI GGGAC 1 cut(s) 355
FatI CATG 4 cut(s) 499, 813, 1122, 1414
FauNDI CATATG 1 cut(s) 226
Fnu4HI GCNGC 6 cut(s) 9, 111, 186, 338, 818, 1209
FokI GGATG 2 cut(s) 566, 1085
FriOI GRGCYC 1 cut(s) 1621
Fsp4HI GCNGC 6 cut(s) 9, 111, 186, 338, 818, 1209
FspBI CTAG 4 cut(s) 287, 374, 1157, 1566
GlaI GCGC 2 cut(s) 248, 1553
GluI GCNGC 6 cut(s) 9, 111, 186, 338, 818, 1209
GsuI CTGGAG 2 cut(s) 1380, 1500
HaeIII GGCC 5 cut(s) 631, 771, 909, 1180, 1285
HapII CCGG 1 cut(s) 1083
HhaI GCGC 2 cut(s) 249, 1554
Hin1II CATG 4 cut(s) 503, 817, 1126, 1418
Hin6I GCGC 2 cut(s) 247, 1552
HinP1I GCGC 2 cut(s) 247, 1552
HincII GTYRAC 1 cut(s) 1315
HindII GTYRAC 1 cut(s) 1315
HindIII AAGCTT 3 cut(s) 270, 347, 509
HinfI GANTC 9 cut(s) 74, 496, 854, 880, 999, 1243, 1253, 1411, 1523
HpaII CCGG 1 cut(s) 1083
HphI GGTGA 6 cut(s) 484, 623, 889, 1106, 1214, 1430
Hpy166II GTNNAC 3 cut(s) 256, 776, 1315
Hpy188I TCNGA 3 cut(s) 1388, 1608, 1638
Hpy188III TCNNGA 8 cut(s) 121, 232, 500, 651, 884, 1072, 1247, 1296
Hpy8I GTNNAC 3 cut(s) 256, 776, 1315
HpyAV CCTTC 4 cut(s) 754, 789, 1399, 1579
HpyCH4III ACNGT 4 cut(s) 463, 485, 1280, 1647
HpyCH4IV ACGT 1 cut(s) 988
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 38, 346, 517, 526, 1205
HpyF3I CTNAG 4 cut(s) 195, 313, 1385, 1635
HpySE526I ACGT 1 cut(s) 988
Hsp92II CATG 4 cut(s) 503, 817, 1126, 1418
HspAI GCGC 2 cut(s) 247, 1552
Kzo9I GATC 4 cut(s) 940, 1168, 1345, 1493
LmnI GCTCC 6 cut(s) 107, 603, 728, 799, 1399, 1616
Lsp1109I GCAGC 5 cut(s) 20, 97, 324, 829, 1220
LweI GCATC 4 cut(s) 1107, 1195, 1318, 1423
MabI ACCWGGT 1 cut(s) 1665
MaeI CTAG 4 cut(s) 287, 374, 1157, 1566
MaeII ACGT 1 cut(s) 988
MaeIII GTNAC 7 cut(s) 80, 289, 457, 946, 976, 1004, 1577
MalI GATC 4 cut(s) 942, 1170, 1347, 1495
MboI GATC 4 cut(s) 940, 1168, 1345, 1493
MflI RGATCY 1 cut(s) 1168
MhlI GDGCHC 2 cut(s) 447, 1621
MluCI AATT 6 cut(s) 114, 579, 657, 845, 1038, 1441
MlyI GAGTC 5 cut(s) 505, 863, 1008, 1252, 1517
MmeI TCCRAC 1 cut(s) 85
Mph1103I ATGCAT 1 cut(s) 367
MroXI GAANNNNTTC 2 cut(s) 715, 1152
MseI TTAA 6 cut(s) 860, 935, 972, 984, 1044, 1107
MslI CAYNNNNRTG 2 cut(s) 225, 1547
MspI CCGG 1 cut(s) 1083
MspR9I CCNGG 2 cut(s) 1095, 1667
Mva1269I GAATGC 1 cut(s) 1242
MvaI CCWGG 2 cut(s) 1095, 1667
MwoI GCNNNNNNNGC 6 cut(s) 29, 38, 346, 517, 526, 1205
NdeI CATATG 1 cut(s) 226
NdeII GATC 4 cut(s) 940, 1168, 1345, 1493
NlaIII CATG 4 cut(s) 503, 817, 1126, 1418
NlaIV GGNNCC 4 cut(s) 605, 1263, 1395, 1618
NmeAIII GCCGAG 1 cut(s) 632
NmuCI GTSAC 4 cut(s) 80, 457, 946, 1577
NsiI ATGCAT 1 cut(s) 367
NspI RCATGY 1 cut(s) 817
NspV TTCGAA 1 cut(s) 758
PaeI GCATGC 1 cut(s) 817
PagI TCATGA 1 cut(s) 499
PaqCI CACCTGC 1 cut(s) 1202
PceI AGGCCT 1 cut(s) 1180
PctI GAATGC 1 cut(s) 1242
PdmI GAANNNNTTC 2 cut(s) 715, 1152
PfeI GAWTC 4 cut(s) 74, 880, 1253, 1411
PinAI ACCGGT 1 cut(s) 1082
PkrI GCNGC 6 cut(s) 10, 112, 187, 339, 819, 1210
PleI GAGTC 5 cut(s) 504, 862, 1007, 1251, 1517
PpsI GAGTC 5 cut(s) 504, 862, 1007, 1251, 1517
Psp6I CCWGG 2 cut(s) 1093, 1665
PspGI CCWGG 2 cut(s) 1093, 1665
PspN4I GGNNCC 4 cut(s) 605, 1263, 1395, 1618
PspPI GGNCC 3 cut(s) 629, 837, 1284
PsuI RGATCY 1 cut(s) 1168
RsaI GTAC 7 cut(s) 62, 354, 482, 694, 894, 1371, 1570
RsaNI GTAC 7 cut(s) 61, 353, 481, 693, 893, 1370, 1569
RseI CAYNNNNRTG 2 cut(s) 225, 1547
SaqAI TTAA 6 cut(s) 860, 935, 972, 984, 1044, 1107
SatI GCNGC 6 cut(s) 9, 111, 186, 338, 818, 1209
Sau3AI GATC 4 cut(s) 940, 1168, 1345, 1493
Sau96I GGNCC 3 cut(s) 629, 837, 1284
ScaI AGTACT 2 cut(s) 482, 694
SchI GAGTC 5 cut(s) 505, 863, 1008, 1252, 1517
ScrFI CCNGG 2 cut(s) 1095, 1667
SduI GDGCHC 2 cut(s) 447, 1621
SexAI ACCWGGT 1 cut(s) 1665
SfaNI GCATC 4 cut(s) 1107, 1195, 1318, 1423
SfuI TTCGAA 1 cut(s) 758
SinI GGWCC 1 cut(s) 837
SmiMI CAYNNNNRTG 2 cut(s) 225, 1547
SpeI ACTAGT 1 cut(s) 1565
SphI GCATGC 1 cut(s) 817
Sse9I AATT 6 cut(s) 114, 579, 657, 845, 1038, 1441
SseBI AGGCCT 1 cut(s) 1180
SsiI CCGC 4 cut(s) 23, 185, 866, 1486
SspI AATATT 1 cut(s) 1153
SspMI CTAG 4 cut(s) 287, 374, 1157, 1566
StuI AGGCCT 1 cut(s) 1180
StyD4I CCNGG 2 cut(s) 1093, 1665
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 4 cut(s) 463, 485, 1280, 1647
TaiI ACGT 1 cut(s) 991
TaqI TCGA 7 cut(s) 137, 231, 405, 641, 758, 960, 1344
TasI AATT 6 cut(s) 114, 579, 657, 845, 1038, 1441
TatI WGTACW 5 cut(s) 60, 480, 692, 892, 1568
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 4 cut(s) 74, 880, 1253, 1411
Tru1I TTAA 6 cut(s) 860, 935, 972, 984, 1044, 1107
Tru9I TTAA 6 cut(s) 860, 935, 972, 984, 1044, 1107
TscAI CASTG 6 cut(s) 214, 580, 742, 1195, 1495, 1582
TseFI GTSAC 4 cut(s) 80, 457, 946, 1577
TseI GCWGC 5 cut(s) 8, 110, 337, 817, 1208
Tsp45I GTSAC 4 cut(s) 80, 457, 946, 1577
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 6 cut(s) 214, 580, 742, 1195, 1495, 1582
VpaK11BI GGWCC 1 cut(s) 837
XagI CCTNNNNNAGG 1 cut(s) 1608
XapI RAATTY 2 cut(s) 1038, 1441
XceI RCATGY 1 cut(s) 817
XmnI GAANNNNTTC 2 cut(s) 715, 1152
XspI CTAG 4 cut(s) 287, 374, 1157, 1566
ZrmI AGTACT 2 cut(s) 482, 694
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.