Rw7G025130

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009135
Physical Location & Seq
Reverse (-)
31400729 .. 31407238
6510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025130.1

Sequence Viewer

Length: 1593 bp
ATGGTTGTTCTTGCGGCTTCCGTTATAAACAAGTCTGGGAAAGTGCTTGTTTCGAGGCAGTTTATGGATATGTCTCGTATAAGGATTGAGGGTCTTCTTGCGGCTTTCCCTAAGTTGGTAGGCACTGGCAAACAACATACATATGTCGAGACGGAAAATGTGCGCTATGTTTACCAGCCAATAGAAGCTTTGTATTTGCTTCTAGTTACAAACAAGCAGAGCAATATCTTAGAAGATTTGGAGACTTTGAGGCTGCTCTCTAAGCTTGTACCTGAATATGCATTGTCCCTAGATGAAGAGGGTATATGCAGGACAGCTTTCGAGTTGATTTTTGCTTTTGATGAAGTAATCTCTCTTGGGCACAAGGAGAATGTGACTGTTGCACAGGTGAAGCAGTACTGTGAGATGGAGAGTCATGAAGAGAAGCTTCACAAGCTCGTAATGCAGAGCAAGATAAATGAGACCAAGGATGTTATGAAGCGTAAAGCCAGTGAGATTGACAAAAGCAAGATTGATAGGAGCAGAGGTGAAAAGGGAGGATTTGGGCCTTTGCAGTCAATGGGTTCTGGAAGAATTGAGAGTGCCTTTAGTGAGATGAGCTTATCCAGCACTGGAAGTGGTTTTGGAAGTGGTTCTGGTTTGGGTGCGACCACTGATTTTGAAACATTTTCTTCGAAGGCCAAAGGCCGTCCACCTTCATCTGCCACAGCTCCCCCAAAGGGTTTAGGCATGCAGCTTGGAAAATCACAGAGGACCAACCAATTTTTAGAGTCCTTAAAAGCGGAGGGAGAGGTGATTCTTGAAGATGTACAGCCAAAAGCAGGCCAGTCAAAATCAGTTGCCCCACCTTTAACTGATCCCGTCACTTTGAATGTCGAAGAGAAACTTAATGTAACACTTAAACGTGATGGTGGAGTCAGTAACTTTGATGTTCAAGGAACATTATCCCTGCAAATTCTTAACCAAGAAGACGGGCATATCCAAGTCCAGATTGAAACCGGTGGAAATCCTGGCATCCTTTTTAAAACTCACCCTAACATGAACAAAGAACTATTTTCTAATGAAAATATTCTAGGCTTGAAAGATCCTAATAGGCCTTTCCCCACTGGTCAAGCTGGTGATGCAGCAGGTGTTGGTCTTTTGAAATGGAGAATGCAGAGTCAAGATGAATCATTGGTGCCTCTACAAATCAACTGTTGGCCCTCTGTTTCTGGAAATGAAACCTATGTCAACATTGAGTATGATGCTTCATCAATGTTCGATCTGCGAAATGTTGTCATTTCAGTACCTCTTCCAGCTCTCAGAGAGGCTCCAGTTGTGAAGGAGATTCATGGTGAATGGAGATATGATGCAAGGAATTCAGTATTGGAGTGGTCTATTCTTCTTATTGATAACTCAAACCGCAGTGGATCAATGGAGTTTGTTGTTCCTCCAGCAGACTCGAATGTATTCTTTCCCATTTCTGTGCGCTTCTCTGCCACTAGTACATTCAGTGACCTGAAGGTTGTGAATATCTTACCTCTGAAAGGTGGAGCCCCTCCGAAGTTCGCTCAGAGAACACAGTTGCTTACAGAGAACTACCAGGTGGTGTAA

Protein Analysis

530

Amino Acids

58.23

Weight (kDa)

5.72

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 285 - 514 2.1e-43 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
AarI CACCTGC 1 cut(s) 1118
Acc36I ACCTGC 1 cut(s) 1118
AccB1I GGYRCC 1 cut(s) 1177
AciI CCGC 4 cut(s) 14, 101, 782, 1402
AclWI GGATC 3 cut(s) 851, 1079, 1417
AcsI RAATTY 2 cut(s) 954, 1357
AcuI CTGAAG 1 cut(s) 1520
AfaI GTAC 5 cut(s) 270, 398, 810, 1287, 1486
AfiI CCNNNNNNNGG 4 cut(s) 115, 719, 821, 1526
AgeI ACCGGT 1 cut(s) 998
AgsI TTSAA 7 cut(s) 662, 803, 871, 935, 995, 1081, 1144
AhlI ACTAGT 1 cut(s) 1481
AjnI CCWGG 2 cut(s) 1009, 1581
AjuI GAANNNNNNNTTGG 2 cut(s) 1349, 1381
Alw26I GTCTC 4 cut(s) 78, 143, 236, 455
AlwI GGATC 3 cut(s) 851, 1079, 1417
AoxI GGCC 6 cut(s) 545, 678, 685, 823, 1094, 1199
ApeKI GCWGC 3 cut(s) 253, 733, 1124
ApoI RAATTY 2 cut(s) 954, 1357
AsiGI ACCGGT 1 cut(s) 998
Asp700I GAANNNNTTC 3 cut(s) 631, 1068, 1448
AspLEI GCGC 2 cut(s) 165, 1470
AspS9I GGNCC 3 cut(s) 545, 753, 1200
AsuHPI GGTGA 6 cut(s) 400, 539, 805, 1022, 1130, 1346
AsuII TTCGAA 1 cut(s) 674
AvaII GGWCC 1 cut(s) 753
BaeGI GKGCMC 1 cut(s) 363
BanI GGYRCC 1 cut(s) 1177
BanII GRGCYC 1 cut(s) 1537
BbsI GAAGAC 2 cut(s) 86, 975
BbvI GCAGC 3 cut(s) 240, 745, 1136
BccI CCATC 2 cut(s) 400, 902
BceAI ACGGC 1 cut(s) 672
BciT130I CCWGG 2 cut(s) 1011, 1583
BcoDI GTCTC 4 cut(s) 78, 143, 236, 455
BcuI ACTAGT 1 cut(s) 1481
BfaI CTAG 4 cut(s) 203, 290, 1073, 1482
BfuAI ACCTGC 1 cut(s) 1118
BisI GCNGC 5 cut(s) 15, 102, 254, 734, 1125
BlsI GCNGC 5 cut(s) 16, 103, 255, 735, 1126
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 2 cut(s) 1011, 1583
Bme18I GGWCC 1 cut(s) 753
BmgT120I GGNCC 3 cut(s) 545, 753, 1200
BmiI GGNNCC 3 cut(s) 1179, 1311, 1534
BmrFI CCNGG 2 cut(s) 1011, 1583
BmsI GCATC 4 cut(s) 1023, 1111, 1234, 1339
BpiI GAAGAC 2 cut(s) 86, 975
BpmI CTGGAG 2 cut(s) 1296, 1416
Bpu14I TTCGAA 1 cut(s) 674
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 465
BsaWI WCCGGW 1 cut(s) 998
BsaXI ACNNNNNCTCC 2 cut(s) 511, 541
Bsc4I CCNNNNNNNGG 4 cut(s) 115, 719, 821, 1526
Bse118I RCCGGY 1 cut(s) 998
Bse1I ACTGG 6 cut(s) 130, 489, 616, 826, 1111, 1313
BseBI CCWGG 2 cut(s) 1011, 1583
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 2 cut(s) 475, 1014
BseLI CCNNNNNNNGG 4 cut(s) 115, 719, 821, 1526
BseMII CTCAG 2 cut(s) 1315, 1565
BseNI ACTGG 6 cut(s) 130, 489, 616, 826, 1111, 1313
BseSI GKGCMC 1 cut(s) 363
BseXI GCAGC 3 cut(s) 240, 745, 1136
BshFI GGCC 6 cut(s) 547, 680, 687, 825, 1096, 1201
BshNI GGYRCC 1 cut(s) 1177
BshTI ACCGGT 1 cut(s) 998
BsiSI CCGG 1 cut(s) 999
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 4 cut(s) 115, 719, 821, 1526
BsmAI GTCTC 4 cut(s) 78, 143, 236, 455
BsmBI CGTCTC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 271
BsmI GAATGC 1 cut(s) 1158
BsnI GGCC 6 cut(s) 547, 680, 687, 825, 1096, 1201
Bso31I GGTCTC 1 cut(s) 455
Bsp119I TTCGAA 1 cut(s) 674
Bsp1286I GDGCHC 2 cut(s) 363, 1537
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 4 cut(s) 856, 1084, 1261, 1409
BspACI CCGC 4 cut(s) 14, 101, 782, 1402
BspANI GGCC 6 cut(s) 547, 680, 687, 825, 1096, 1201
BspCNI CTCAG 2 cut(s) 1314, 1564
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 1179, 1311, 1534
BspMI ACCTGC 1 cut(s) 1118
BspPI GGATC 3 cut(s) 851, 1079, 1417
BspT104I TTCGAA 1 cut(s) 674
BspT107I GGYRCC 1 cut(s) 1177
BspTNI GGTCTC 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 998
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 6 cut(s) 130, 489, 616, 826, 1111, 1313
BssAI RCCGGY 1 cut(s) 998
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 4 cut(s) 856, 1084, 1261, 1409
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 2 cut(s) 1011, 1583
Bst4CI ACNGT 4 cut(s) 379, 401, 1196, 1563
Bst6I CTCTTC 4 cut(s) 291, 414, 873, 1296
BstAUI TGTACA 1 cut(s) 808
BstBI TTCGAA 1 cut(s) 674
BstC8I GCNNGC 2 cut(s) 731, 823
BstDEI CTNAG 5 cut(s) 111, 229, 261, 1301, 1551
BstENI CCTNNNNNAGG 1 cut(s) 1524
BstF5I GGATG 2 cut(s) 475, 1014
BstHHI GCGC 2 cut(s) 165, 1470
BstKTI GATC 4 cut(s) 859, 1087, 1264, 1412
BstMAI GTCTC 4 cut(s) 78, 143, 236, 455
BstMBI GATC 4 cut(s) 856, 1084, 1261, 1409
BstMWI GCNNNNNNNGC 5 cut(s) 262, 433, 442, 606, 1121
BstNI CCWGG 2 cut(s) 1011, 1583
BstNSI RCATGY 1 cut(s) 733
BstSCI CCNGG 2 cut(s) 1009, 1581
BstSLI GKGCMC 1 cut(s) 363
BstV1I GCAGC 3 cut(s) 240, 745, 1136
BstV2I GAAGAC 2 cut(s) 86, 975
BstX2I RGATCY 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1084
BsuRI GGCC 6 cut(s) 547, 680, 687, 825, 1096, 1201
BtsCI GGATG 2 cut(s) 475, 1014
BtsI GCAGTG 1 cut(s) 1411
BtsIMutI CAGTG 7 cut(s) 123, 496, 609, 651, 1104, 1411, 1498
BveI ACCTGC 1 cut(s) 1118
Cac8I GCNNGC 2 cut(s) 731, 823
CciI TCATGA 1 cut(s) 415
CfoI GCGC 2 cut(s) 165, 1470
Cfr10I RCCGGY 1 cut(s) 998
Cfr13I GGNCC 3 cut(s) 545, 753, 1200
CsiI ACCWGGT 1 cut(s) 1581
Csp6I GTAC 5 cut(s) 269, 397, 809, 1286, 1485
CspAI ACCGGT 1 cut(s) 998
CspCI CAANNNNNGTGG 2 cut(s) 640, 675
CviAII CATG 4 cut(s) 416, 730, 1039, 1331
CviQI GTAC 5 cut(s) 269, 397, 809, 1286, 1485
DdeI CTNAG 5 cut(s) 111, 229, 261, 1301, 1551
DpnI GATC 4 cut(s) 858, 1086, 1263, 1411
DpnII GATC 4 cut(s) 856, 1084, 1261, 1409
DraI TTTAAA 1 cut(s) 1024
Eam1104I CTCTTC 4 cut(s) 291, 414, 873, 1296
EarI CTCTTC 4 cut(s) 291, 414, 873, 1296
Eco130I CCWWGG 1 cut(s) 465
Eco147I AGGCCT 1 cut(s) 1096
Eco24I GRGCYC 1 cut(s) 1537
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 753
Eco57I CTGAAG 1 cut(s) 1520
EcoNI CCTNNNNNAGG 1 cut(s) 1524
EcoRI GAATTC 1 cut(s) 1357
EcoRII CCWGG 2 cut(s) 1009, 1581
EcoT14I CCWWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 1537
ErhI CCWWGG 1 cut(s) 465
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 4 cut(s) 419, 733, 1042, 1334
FalI AAGNNNNNCTT 4 cut(s) 411, 443, 870, 902
FaqI GGGAC 1 cut(s) 271
FatI CATG 4 cut(s) 415, 729, 1038, 1330
FauNDI CATATG 1 cut(s) 142
Fnu4HI GCNGC 5 cut(s) 15, 102, 254, 734, 1125
FokI GGATG 2 cut(s) 482, 1001
FriOI GRGCYC 1 cut(s) 1537
Fsp4HI GCNGC 5 cut(s) 15, 102, 254, 734, 1125
FspBI CTAG 4 cut(s) 203, 290, 1073, 1482
GlaI GCGC 2 cut(s) 164, 1469
GluI GCNGC 5 cut(s) 15, 102, 254, 734, 1125
GsuI CTGGAG 2 cut(s) 1296, 1416
HaeIII GGCC 6 cut(s) 547, 680, 687, 825, 1096, 1201
HapII CCGG 1 cut(s) 999
HhaI GCGC 2 cut(s) 165, 1470
Hin1II CATG 4 cut(s) 419, 733, 1042, 1334
Hin6I GCGC 2 cut(s) 163, 1468
HinP1I GCGC 2 cut(s) 163, 1468
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HindIII AAGCTT 3 cut(s) 186, 263, 425
HinfI GANTC 8 cut(s) 412, 770, 796, 915, 1159, 1169, 1327, 1439
HpaII CCGG 1 cut(s) 999
HphI GGTGA 6 cut(s) 400, 539, 805, 1022, 1130, 1346
Hpy166II GTNNAC 3 cut(s) 172, 692, 1231
Hpy188I TCNGA 4 cut(s) 1304, 1524, 1542, 1554
Hpy188III TCNNGA 7 cut(s) 148, 416, 567, 800, 988, 1163, 1212
Hpy8I GTNNAC 3 cut(s) 172, 692, 1231
HpyAV CCTTC 4 cut(s) 670, 705, 1315, 1495
HpyCH4III ACNGT 4 cut(s) 379, 401, 1196, 1563
HpyCH4IV ACGT 1 cut(s) 904
HpyF10VI GCNNNNNNNGC 5 cut(s) 262, 433, 442, 606, 1121
HpyF3I CTNAG 5 cut(s) 111, 229, 261, 1301, 1551
HpySE526I ACGT 1 cut(s) 904
Hsp92II CATG 4 cut(s) 419, 733, 1042, 1334
HspAI GCGC 2 cut(s) 163, 1468
Kzo9I GATC 4 cut(s) 856, 1084, 1261, 1409
LmnI GCTCC 4 cut(s) 519, 715, 1315, 1532
Lsp1109I GCAGC 3 cut(s) 240, 745, 1136
LweI GCATC 4 cut(s) 1023, 1111, 1234, 1339
MabI ACCWGGT 1 cut(s) 1581
MaeI CTAG 4 cut(s) 203, 290, 1073, 1482
MaeII ACGT 1 cut(s) 904
MaeIII GTNAC 6 cut(s) 205, 373, 862, 892, 920, 1493
MalI GATC 4 cut(s) 858, 1086, 1263, 1411
MboI GATC 4 cut(s) 856, 1084, 1261, 1409
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 2 cut(s) 363, 1537
MluCI AATT 4 cut(s) 573, 761, 954, 1357
MlyI GAGTC 5 cut(s) 421, 779, 924, 1168, 1433
Mph1103I ATGCAT 1 cut(s) 283
MroXI GAANNNNTTC 3 cut(s) 631, 1068, 1448
MseI TTAA 6 cut(s) 776, 851, 888, 900, 960, 1023
MslI CAYNNNNRTG 2 cut(s) 141, 1463
MspI CCGG 1 cut(s) 999
MspR9I CCNGG 2 cut(s) 1011, 1583
Mva1269I GAATGC 1 cut(s) 1158
MvaI CCWGG 2 cut(s) 1011, 1583
MwoI GCNNNNNNNGC 5 cut(s) 262, 433, 442, 606, 1121
NdeI CATATG 1 cut(s) 142
NdeII GATC 4 cut(s) 856, 1084, 1261, 1409
NlaIII CATG 4 cut(s) 419, 733, 1042, 1334
NlaIV GGNNCC 3 cut(s) 1179, 1311, 1534
NmuCI GTSAC 3 cut(s) 373, 862, 1493
NsiI ATGCAT 1 cut(s) 283
NspI RCATGY 1 cut(s) 733
NspV TTCGAA 1 cut(s) 674
PaeI GCATGC 1 cut(s) 733
PagI TCATGA 1 cut(s) 415
PaqCI CACCTGC 1 cut(s) 1118
PceI AGGCCT 1 cut(s) 1096
PctI GAATGC 1 cut(s) 1158
PdmI GAANNNNTTC 3 cut(s) 631, 1068, 1448
PfeI GAWTC 3 cut(s) 796, 1169, 1327
PinAI ACCGGT 1 cut(s) 998
PkrI GCNGC 5 cut(s) 16, 103, 255, 735, 1126
PleI GAGTC 5 cut(s) 420, 778, 923, 1167, 1433
PpsI GAGTC 5 cut(s) 420, 778, 923, 1167, 1433
PsiI TTATAA 1 cut(s) 26
Psp6I CCWGG 2 cut(s) 1009, 1581
PspGI CCWGG 2 cut(s) 1009, 1581
PspN4I GGNNCC 3 cut(s) 1179, 1311, 1534
PspPI GGNCC 3 cut(s) 545, 753, 1200
PsuI RGATCY 1 cut(s) 1084
RsaI GTAC 5 cut(s) 270, 398, 810, 1287, 1486
RsaNI GTAC 5 cut(s) 269, 397, 809, 1286, 1485
RseI CAYNNNNRTG 2 cut(s) 141, 1463
SaqAI TTAA 6 cut(s) 776, 851, 888, 900, 960, 1023
SatI GCNGC 5 cut(s) 15, 102, 254, 734, 1125
Sau3AI GATC 4 cut(s) 856, 1084, 1261, 1409
Sau96I GGNCC 3 cut(s) 545, 753, 1200
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 5 cut(s) 421, 779, 924, 1168, 1433
ScrFI CCNGG 2 cut(s) 1011, 1583
SduI GDGCHC 2 cut(s) 363, 1537
SexAI ACCWGGT 1 cut(s) 1581
SfaNI GCATC 4 cut(s) 1023, 1111, 1234, 1339
SfuI TTCGAA 1 cut(s) 674
SinI GGWCC 1 cut(s) 753
SmiMI CAYNNNNRTG 2 cut(s) 141, 1463
SpeI ACTAGT 1 cut(s) 1481
SphI GCATGC 1 cut(s) 733
Sse9I AATT 4 cut(s) 573, 761, 954, 1357
SseBI AGGCCT 1 cut(s) 1096
SsiI CCGC 4 cut(s) 14, 101, 782, 1402
SspI AATATT 1 cut(s) 1069
SspMI CTAG 4 cut(s) 203, 290, 1073, 1482
StuI AGGCCT 1 cut(s) 1096
StyD4I CCNGG 2 cut(s) 1009, 1581
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 4 cut(s) 379, 401, 1196, 1563
TaiI ACGT 1 cut(s) 907
TaqI TCGA 7 cut(s) 53, 147, 321, 674, 876, 1260, 1442
TasI AATT 4 cut(s) 573, 761, 954, 1357
TatI WGTACW 3 cut(s) 396, 808, 1484
TauI GCSGC 2 cut(s) 17, 104
TfiI GAWTC 3 cut(s) 796, 1169, 1327
Tru1I TTAA 6 cut(s) 776, 851, 888, 900, 960, 1023
Tru9I TTAA 6 cut(s) 776, 851, 888, 900, 960, 1023
TscAI CASTG 7 cut(s) 130, 496, 616, 658, 1111, 1411, 1498
TseFI GTSAC 3 cut(s) 373, 862, 1493
TseI GCWGC 3 cut(s) 253, 733, 1124
Tsp45I GTSAC 3 cut(s) 373, 862, 1493
TspGWI ACGGA 2 cut(s) 10, 167
TspRI CASTG 7 cut(s) 130, 496, 616, 658, 1111, 1411, 1498
VpaK11BI GGWCC 1 cut(s) 753
XagI CCTNNNNNAGG 1 cut(s) 1524
XapI RAATTY 2 cut(s) 954, 1357
XceI RCATGY 1 cut(s) 733
XmnI GAANNNNTTC 3 cut(s) 631, 1068, 1448
XspI CTAG 4 cut(s) 203, 290, 1073, 1482
ZrmI AGTACT 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.