Rh7AG295500

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0009135
Physical Location & Seq
Reverse (-)
32342531 .. 32362122
19592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG295500.1

Sequence Viewer

Length: 2337 bp
ATGAGCTGTCTCCCAAAGCTTGGAAATGATTTGCAAAAGTGCCGAGCCAAAGGCTTGATGTCAGAGCATCAGAGGATTCAAGTGATAATTTGCTGCCAGTTGCTCCTCTTCAGTACAGATAGCTTAAAAGACAGCTTGAAGAAAGTTGAAGATAAGTTAATTATTGCCAATTCTGAGGCCTTTACTCTGAGCGAAAAGGTAAGTTCGCTTGAAGATGAAATAAGTGAAATGGGAAATATTATTACAGATCTTAAAGAGAAAGAATCAAAAGCTGAAGGTAGGGCTTGGAATGCAGAAGCCAAATGCAATTTATTAACAGAGACCAGCGCTGGACTTAATGAAGAGCTCAGACTTCTTAAGACGCAGTTAACAGAATTTGACATGCGGTTGCAACATGCAGTGGCAACTGTTGGAGCTAGCCAGGAGAAGCAAATCATGTTGAATTCGATTATCATACATATGGAAAATGTGATAAAGGATCTAAAGCAGAAGGTTTCAAACGCTGAAAGTCGGGCTGACAGTGTAGAGGAAAAGTGTATAATAATATCAAAATCCAATGCAGCACTAAATGATAAAGTAAGCTTTCTGACGGGTAGATTGAAATGCATGGAGGCAGCTTTGTATCAAGCGGAGGAAGCAAAAATAGCTATTGCAACTGTTATTGAGTACATTGCAACTGAATCTGGTCACATATTTCAAGCAGTTTTCATTGGAGCTGCAATTTTCCCGATAGGTATGCTCATGTTTGTTGTTCTTGCGGCTTCCGTTATAAACAAGTCTGGGAAAGTGCTTGTTTCGAGGCAGTTTATGGATATGTCTCGTATAAGGATTGAGGGTCTTCTTGCGGCTTTCCCTAAGTTGGTAGGCACTGGAAAACAACATACATATGTCGAGACGGAAAATGTGCGCTATGTTTACCAGCCAATAGAAGCTTTGTATTTGCTTCTAGTTACAAACAAGCAGAGCAATATCTTAGAAGATTTGGAGACTTTGAGGCTGCTCTCTAAGCTTGTGCCTGAATATGCATTGTCCCTAGATGAAGAGGGTATATGCAGGACAGCTTTCGAGTTGATTTTTGCTTTTGATGAAGTAATCTCTCTTGGGCACAAGGAGAATGTGACTGTTGCACAGGTGAAGCAGTACTGTGAGATGGAGAGTCATGAAGAGAAGCTTCACAAGCTCGTAATGCAGAGCAAGATAAATGAGACCAAGGATGTTATGAAGCGTAAAGCCAGTGAGATTGACAAAAGCAAGATTGATAGGAGCAGAGGTGAAAAGGGAGGATTTGGGCCTTTGCAGTCAATGGGTTCTGGAAGAATTGAGAGTGCCTTTAGTGAGATGAGCTTATCCAGCACTGGAAGTGGTTTTGGAAGTGGTTCTGGTTTGGGAGCGACCGCTGATTTTGAAACATTTTCTTCGAAGGCCAAAGGCCGTCCACCTTCATCTGCCACAGCTCCGCCAAAGGGTTTAGGCATGCAGCTTGGAAAATCACAAAGGACCAACCAATTTTTAGAGTCCTTAAAAGCGGAGGGAGAGGTGATCCTTGAAGATGTACAGCCAAAAGCAGGCCAGTCAAAATTGGTTGCCCCACCTTTAACTGATCCCGTCACTTTGAATGTCGAAGAGAAACTTAATGTAACACTTAAACGTGATGGTGGAGTCAGTAACTTTGATGTTCAAGGAACATTATCCCTGCAAATTCTTAACCAAGAAGACGGGCATATCCAAGTCCAGATTGAAACCGGTGGAAATCCTGGCATCCTTTTTAAAACTCATCCTAACATGAACAAAGAACTATTTTCTAATGAAAATATTCTAGGCTTGAAAGATCCTAATAGGCCTTTCCCCACTGGTCAAGCTGGTGATGCAGCAGGTGTTGGTCTTTTGAAATGGAGAATGCAGAGTCAAGATGAATCATTGGTGCCTCTACAAATCAACTGTTGGCCCTCTGTTTCTGGAAATGAAACCTATGTCAACATTGAGTATGATGCTTCATCAATGTTCGATCTGCGAAATGTTCTCCTTTCAGTTCCTCTTCCAGCTCTCAGAGAGGCTCCAGTTGTGAAGGAGATTCATGGTGAATGGAGATATGATGCAAGGAATTCAGTATTGGAGTGGTCTATACTTCTTATTGATAACTCAAACCGCAGTGGATCAATGGAGTTTGTTGTTCCTCCAGCAGACTCGAATGTATTCTTTCCCATTTCTGTGCGCTTCTCTGCCACTAGTACATTCAGTGACCTGAAGGTTGTGAATATCTTACCTCTGAAAGGTGGAGCCCCTCCCAAGTTCGCTCAGAGAACACAGTTGCTCACAGAGAACTACCAGGTGGTGTAA

Protein Analysis

778

Amino Acids

85.66

Weight (kDa)

5.6

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 533 - 762 9.4e-42 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 770
AarI CACCTGC 1 cut(s) 1862
Acc36I ACCTGC 1 cut(s) 1862
AccB1I GGYRCC 1 cut(s) 1921
AccB7I CCANNNNNTGG 1 cut(s) 20
AciI CCGC 8 cut(s) 385, 629, 758, 845, 1395, 1457, 1526, 2146
AclWI GGATC 5 cut(s) 486, 1534, 1595, 1823, 2161
AcsI RAATTY 4 cut(s) 374, 442, 1698, 2101
AcuI CTGAAG 3 cut(s) 94, 294, 2264
AfaI GTAC 5 cut(s) 115, 668, 1142, 1554, 2230
AfeI AGCGCT 1 cut(s) 328
AfiI CCNNNNNNNGG 5 cut(s) 20, 859, 1463, 1565, 2270
AflII CTTAAG 1 cut(s) 356
AgeI ACCGGT 1 cut(s) 1742
AhlI ACTAGT 1 cut(s) 2225
AjnI CCWGG 3 cut(s) 420, 1753, 2325
AjuI GAANNNNNNNTTGG 2 cut(s) 2093, 2125
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 6 cut(s) 14, 314, 822, 887, 980, 1199
AlwI GGATC 5 cut(s) 486, 1534, 1595, 1823, 2161
Aor51HI AGCGCT 1 cut(s) 328
AoxI GGCC 7 cut(s) 177, 1289, 1422, 1429, 1567, 1838, 1943
ApeKI GCWGC 7 cut(s) 93, 560, 614, 716, 997, 1477, 1868
ApoI RAATTY 4 cut(s) 374, 442, 1698, 2101
AsiGI ACCGGT 1 cut(s) 1742
Asp700I GAANNNNTTC 3 cut(s) 1375, 1812, 2192
AspLEI GCGC 3 cut(s) 329, 909, 2214
AspS9I GGNCC 3 cut(s) 1289, 1497, 1944
AsuHPI GGTGA 5 cut(s) 1144, 1283, 1549, 1874, 2090
AsuII TTCGAA 1 cut(s) 1418
AsuNHI GCTAGC 1 cut(s) 416
AvaII GGWCC 1 cut(s) 1497
BaeGI GKGCMC 1 cut(s) 1107
BanI GGYRCC 1 cut(s) 1921
BanII GRGCYC 2 cut(s) 348, 2281
BbsI GAAGAC 2 cut(s) 830, 1719
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 7 cut(s) 80, 572, 626, 703, 984, 1489, 1880
BccI CCATC 2 cut(s) 1144, 1646
BceAI ACGGC 1 cut(s) 1416
BcgI CGANNNNNNTGC 2 cut(s) 718, 752
BciT130I CCWGG 3 cut(s) 422, 1755, 2327
BcoDI GTCTC 6 cut(s) 14, 314, 822, 887, 980, 1199
BcuI ACTAGT 1 cut(s) 2225
BfaI CTAG 5 cut(s) 417, 947, 1034, 1817, 2226
BfoI RGCGCY 1 cut(s) 330
BfrI CTTAAG 1 cut(s) 356
BfuAI ACCTGC 1 cut(s) 1862
BglII AGATCT 1 cut(s) 247
BisI GCNGC 9 cut(s) 94, 561, 615, 717, 759, 846, 998, 1478, 1869
BlsI GCNGC 9 cut(s) 95, 562, 616, 718, 760, 847, 999, 1479, 1870
BmcAI AGTACT 1 cut(s) 1142
Bme1390I CCNGG 3 cut(s) 422, 1755, 2327
Bme18I GGWCC 1 cut(s) 1497
BmgT120I GGNCC 3 cut(s) 1289, 1497, 1944
BmiI GGNNCC 3 cut(s) 1923, 2055, 2278
BmrFI CCNGG 3 cut(s) 422, 1755, 2327
BmsI GCATC 5 cut(s) 76, 1767, 1855, 1978, 2083
BmtI GCTAGC 1 cut(s) 420
BpiI GAAGAC 2 cut(s) 830, 1719
BpmI CTGGAG 2 cut(s) 2040, 2160
Bpu14I TTCGAA 1 cut(s) 1418
BsaI GGTCTC 2 cut(s) 314, 1199
BsaJI CCNNGG 1 cut(s) 1209
BsaWI WCCGGW 1 cut(s) 1742
BsaXI ACNNNNNCTCC 2 cut(s) 1255, 1285
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 859, 1463, 1565, 2270
Bse118I RCCGGY 1 cut(s) 1742
Bse1I ACTGG 7 cut(s) 97, 874, 1233, 1360, 1570, 1855, 2057
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 3 cut(s) 422, 1755, 2327
BseDI CCNNGG 1 cut(s) 1209
BseGI GGATG 3 cut(s) 1219, 1758, 1774
BseLI CCNNNNNNNGG 5 cut(s) 20, 859, 1463, 1565, 2270
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 5 cut(s) 165, 179, 361, 2059, 2309
BseNI ACTGG 7 cut(s) 97, 874, 1233, 1360, 1570, 1855, 2057
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 1107
BseXI GCAGC 7 cut(s) 80, 572, 626, 703, 984, 1489, 1880
Bsh1285I CGRYCG 1 cut(s) 1395
BshFI GGCC 7 cut(s) 179, 1291, 1424, 1431, 1569, 1840, 1945
BshNI GGYRCC 1 cut(s) 1921
BshTI ACCGGT 1 cut(s) 1742
BsiEI CGRYCG 1 cut(s) 1395
BsiHKAI GWGCWC 1 cut(s) 348
BsiSI CCGG 1 cut(s) 1743
BslFI GGGAC 1 cut(s) 1015
BslI CCNNNNNNNGG 5 cut(s) 20, 859, 1463, 1565, 2270
BsmAI GTCTC 6 cut(s) 14, 314, 822, 887, 980, 1199
BsmBI CGTCTC 1 cut(s) 887
BsmFI GGGAC 1 cut(s) 1015
BsmI GAATGC 2 cut(s) 295, 1902
BsnI GGCC 7 cut(s) 179, 1291, 1424, 1431, 1569, 1840, 1945
Bso31I GGTCTC 2 cut(s) 314, 1199
Bsp119I TTCGAA 1 cut(s) 1418
Bsp1286I GDGCHC 3 cut(s) 348, 1107, 2281
Bsp1407I TGTACA 1 cut(s) 1552
Bsp143I GATC 7 cut(s) 247, 478, 1539, 1600, 1828, 2005, 2153
BspACI CCGC 8 cut(s) 385, 629, 758, 845, 1395, 1457, 1526, 2146
BspANI GGCC 7 cut(s) 179, 1291, 1424, 1431, 1569, 1840, 1945
BspCNI CTCAG 5 cut(s) 166, 180, 360, 2058, 2308
BspHI TCATGA 1 cut(s) 1159
BspLI GGNNCC 3 cut(s) 1923, 2055, 2278
BspMI ACCTGC 1 cut(s) 1862
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 5 cut(s) 486, 1534, 1595, 1823, 2161
BspQI GCTCTTC 1 cut(s) 336
BspT104I TTCGAA 1 cut(s) 1418
BspT107I GGYRCC 1 cut(s) 1921
BspTI CTTAAG 1 cut(s) 356
BspTNI GGTCTC 2 cut(s) 314, 1199
BsrDI GCAATG 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 1742
BsrGI TGTACA 1 cut(s) 1552
BsrI ACTGG 7 cut(s) 97, 874, 1233, 1360, 1570, 1855, 2057
BssAI RCCGGY 1 cut(s) 1742
BssECI CCNNGG 1 cut(s) 1209
BssMI GATC 7 cut(s) 247, 478, 1539, 1600, 1828, 2005, 2153
BssT1I CCWWGG 1 cut(s) 1209
Bst2UI CCWGG 3 cut(s) 422, 1755, 2327
Bst4CI ACNGT 7 cut(s) 409, 521, 658, 1123, 1145, 1940, 2307
Bst6I CTCTTC 6 cut(s) 113, 336, 1035, 1158, 1617, 2040
BstAFI CTTAAG 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 1552
BstBI TTCGAA 1 cut(s) 1418
BstC8I GCNNGC 3 cut(s) 418, 1475, 1567
BstDEI CTNAG 8 cut(s) 174, 188, 347, 855, 973, 1005, 2045, 2295
BstENI CCTNNNNNAGG 1 cut(s) 2268
BstF5I GGATG 3 cut(s) 1219, 1758, 1774
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 3 cut(s) 329, 909, 2214
BstKTI GATC 7 cut(s) 250, 481, 1542, 1603, 1831, 2008, 2156
BstMAI GTCTC 6 cut(s) 14, 314, 822, 887, 980, 1199
BstMBI GATC 7 cut(s) 247, 478, 1539, 1600, 1828, 2005, 2153
BstMCI CGRYCG 1 cut(s) 1395
BstMWI GCNNNNNNNGC 8 cut(s) 290, 635, 644, 1006, 1177, 1186, 1350, 1865
BstNI CCWGG 3 cut(s) 422, 1755, 2327
BstNSI RCATGY 3 cut(s) 385, 398, 1477
BstSCI CCNGG 3 cut(s) 420, 1753, 2325
BstSLI GKGCMC 1 cut(s) 1107
BstV1I GCAGC 7 cut(s) 80, 572, 626, 703, 984, 1489, 1880
BstV2I GAAGAC 2 cut(s) 830, 1719
BstX2I RGATCY 3 cut(s) 247, 478, 1828
BstYI RGATCY 3 cut(s) 247, 478, 1828
BsuRI GGCC 7 cut(s) 179, 1291, 1424, 1431, 1569, 1840, 1945
BtsCI GGATG 3 cut(s) 1219, 1758, 1774
BtsI GCAGTG 2 cut(s) 405, 2155
BtsIMutI CAGTG 8 cut(s) 405, 526, 867, 1240, 1353, 1848, 2155, 2242
BveI ACCTGC 1 cut(s) 1862
Cac8I GCNNGC 3 cut(s) 418, 1475, 1567
CciI TCATGA 1 cut(s) 1159
CfoI GCGC 3 cut(s) 329, 909, 2214
Cfr10I RCCGGY 1 cut(s) 1742
Cfr13I GGNCC 3 cut(s) 1289, 1497, 1944
CseI GACGC 1 cut(s) 370
CsiI ACCWGGT 1 cut(s) 2325
Csp6I GTAC 5 cut(s) 114, 667, 1141, 1553, 2229
CspAI ACCGGT 1 cut(s) 1742
CviAII CATG 9 cut(s) 382, 395, 436, 607, 742, 1160, 1474, 1783, 2075
CviQI GTAC 5 cut(s) 114, 667, 1141, 1553, 2229
DdeI CTNAG 8 cut(s) 174, 188, 347, 855, 973, 1005, 2045, 2295
DpnI GATC 7 cut(s) 249, 480, 1541, 1602, 1830, 2007, 2155
DpnII GATC 7 cut(s) 247, 478, 1539, 1600, 1828, 2005, 2153
DraI TTTAAA 1 cut(s) 1768
Eam1104I CTCTTC 6 cut(s) 113, 336, 1035, 1158, 1617, 2040
EarI CTCTTC 6 cut(s) 113, 336, 1035, 1158, 1617, 2040
EciI GGCGGA 1 cut(s) 1446
Ecl136II GAGCTC 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 1209
Eco147I AGGCCT 2 cut(s) 179, 1840
Eco24I GRGCYC 2 cut(s) 348, 2281
Eco31I GGTCTC 2 cut(s) 314, 1199
Eco47I GGWCC 1 cut(s) 1497
Eco47III AGCGCT 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 346
Eco57I CTGAAG 3 cut(s) 94, 294, 2264
EcoICRI GAGCTC 1 cut(s) 346
EcoNI CCTNNNNNAGG 1 cut(s) 2268
EcoRI GAATTC 2 cut(s) 442, 2101
EcoRII CCWGG 3 cut(s) 420, 1753, 2325
EcoT14I CCWWGG 1 cut(s) 1209
EcoT22I ATGCAT 2 cut(s) 608, 1027
EcoT38I GRGCYC 2 cut(s) 348, 2281
ErhI CCWWGG 1 cut(s) 1209
Esp3I CGTCTC 1 cut(s) 887
FaeI CATG 9 cut(s) 385, 398, 439, 610, 745, 1163, 1477, 1786, 2078
FalI AAGNNNNNCTT 4 cut(s) 1155, 1187, 1614, 1646
FaqI GGGAC 1 cut(s) 1015
FatI CATG 9 cut(s) 381, 394, 435, 606, 741, 1159, 1473, 1782, 2074
FauNDI CATATG 2 cut(s) 459, 886
Fnu4HI GCNGC 9 cut(s) 94, 561, 615, 717, 759, 846, 998, 1478, 1869
FokI GGATG 3 cut(s) 1226, 1745, 1761
FriOI GRGCYC 2 cut(s) 348, 2281
Fsp4HI GCNGC 9 cut(s) 94, 561, 615, 717, 759, 846, 998, 1478, 1869
FspBI CTAG 5 cut(s) 417, 947, 1034, 1817, 2226
GlaI GCGC 3 cut(s) 328, 908, 2213
GluI GCNGC 9 cut(s) 94, 561, 615, 717, 759, 846, 998, 1478, 1869
GsuI CTGGAG 2 cut(s) 2040, 2160
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 7 cut(s) 179, 1291, 1424, 1431, 1569, 1840, 1945
HapII CCGG 1 cut(s) 1743
HgaI GACGC 1 cut(s) 370
HhaI GCGC 3 cut(s) 329, 909, 2214
Hin1II CATG 9 cut(s) 385, 398, 439, 610, 745, 1163, 1477, 1786, 2078
Hin6I GCGC 3 cut(s) 327, 907, 2212
HinP1I GCGC 3 cut(s) 327, 907, 2212
HincII GTYRAC 2 cut(s) 369, 1975
HindII GTYRAC 2 cut(s) 369, 1975
HindIII AAGCTT 5 cut(s) 17, 580, 930, 1007, 1169
HpaI GTTAAC 1 cut(s) 369
HpaII CCGG 1 cut(s) 1743
HphI GGTGA 5 cut(s) 1144, 1283, 1549, 1874, 2090
Hpy166II GTNNAC 4 cut(s) 369, 916, 1436, 1975
Hpy188I TCNGA 9 cut(s) 64, 72, 175, 189, 350, 588, 2048, 2268, 2298
Hpy188III TCNNGA 7 cut(s) 727, 892, 1160, 1311, 1732, 1907, 1956
Hpy8I GTNNAC 4 cut(s) 369, 916, 1436, 1975
HpyAV CCTTC 6 cut(s) 269, 484, 1414, 1449, 2059, 2239
HpyCH4III ACNGT 7 cut(s) 409, 521, 658, 1123, 1145, 1940, 2307
HpyCH4IV ACGT 1 cut(s) 1648
HpyF10VI GCNNNNNNNGC 8 cut(s) 290, 635, 644, 1006, 1177, 1186, 1350, 1865
HpyF3I CTNAG 8 cut(s) 174, 188, 347, 855, 973, 1005, 2045, 2295
HpySE526I ACGT 1 cut(s) 1648
Hsp92II CATG 9 cut(s) 385, 398, 439, 610, 745, 1163, 1477, 1786, 2078
HspAI GCGC 3 cut(s) 327, 907, 2212
KspAI GTTAAC 1 cut(s) 369
Kzo9I GATC 7 cut(s) 247, 478, 1539, 1600, 1828, 2005, 2153
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 8 cut(s) 108, 413, 713, 1263, 1388, 1459, 2059, 2276
Lsp1109I GCAGC 7 cut(s) 80, 572, 626, 703, 984, 1489, 1880
LweI GCATC 5 cut(s) 76, 1767, 1855, 1978, 2083
MabI ACCWGGT 1 cut(s) 2325
MaeI CTAG 5 cut(s) 417, 947, 1034, 1817, 2226
MaeII ACGT 1 cut(s) 1648
MaeIII GTNAC 7 cut(s) 686, 949, 1117, 1606, 1636, 1664, 2237
MalI GATC 7 cut(s) 249, 480, 1541, 1602, 1830, 2007, 2155
MboI GATC 7 cut(s) 247, 478, 1539, 1600, 1828, 2005, 2153
MflI RGATCY 3 cut(s) 247, 478, 1828
MhlI GDGCHC 3 cut(s) 348, 1107, 2281
MlyI GAGTC 5 cut(s) 1165, 1523, 1668, 1912, 2177
MmeI TCCRAC 1 cut(s) 391
Mph1103I ATGCAT 2 cut(s) 608, 1027
MroXI GAANNNNTTC 3 cut(s) 1375, 1812, 2192
MslI CAYNNNNRTG 3 cut(s) 458, 885, 2207
MspA1I CMGCKG 1 cut(s) 1397
MspCI CTTAAG 1 cut(s) 356
MspI CCGG 1 cut(s) 1743
MspR9I CCNGG 3 cut(s) 422, 1755, 2327
Mva1269I GAATGC 2 cut(s) 295, 1902
MvaI CCWGG 3 cut(s) 422, 1755, 2327
MwoI GCNNNNNNNGC 8 cut(s) 290, 635, 644, 1006, 1177, 1186, 1350, 1865
NdeI CATATG 2 cut(s) 459, 886
NdeII GATC 7 cut(s) 247, 478, 1539, 1600, 1828, 2005, 2153
NheI GCTAGC 1 cut(s) 416
NlaIII CATG 9 cut(s) 385, 398, 439, 610, 745, 1163, 1477, 1786, 2078
NlaIV GGNNCC 3 cut(s) 1923, 2055, 2278
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 4 cut(s) 686, 1117, 1606, 2237
NsiI ATGCAT 2 cut(s) 608, 1027
NspI RCATGY 3 cut(s) 385, 398, 1477
NspV TTCGAA 1 cut(s) 1418
PaeI GCATGC 1 cut(s) 1477
PagI TCATGA 1 cut(s) 1159
PaqCI CACCTGC 1 cut(s) 1862
PceI AGGCCT 2 cut(s) 179, 1840
PciSI GCTCTTC 1 cut(s) 336
PctI GAATGC 2 cut(s) 295, 1902
PdmI GAANNNNTTC 3 cut(s) 1375, 1812, 2192
PfeI GAWTC 5 cut(s) 76, 263, 680, 1913, 2071
PflMI CCANNNNNTGG 1 cut(s) 20
PinAI ACCGGT 1 cut(s) 1742
PkrI GCNGC 9 cut(s) 95, 562, 616, 718, 760, 847, 999, 1479, 1870
PleI GAGTC 5 cut(s) 1164, 1522, 1667, 1911, 2177
PpsI GAGTC 5 cut(s) 1164, 1522, 1667, 1911, 2177
PsiI TTATAA 1 cut(s) 770
Psp124BI GAGCTC 1 cut(s) 348
Psp6I CCWGG 3 cut(s) 420, 1753, 2325
PspGI CCWGG 3 cut(s) 420, 1753, 2325
PspN4I GGNNCC 3 cut(s) 1923, 2055, 2278
PspPI GGNCC 3 cut(s) 1289, 1497, 1944
PsuI RGATCY 3 cut(s) 247, 478, 1828
RsaI GTAC 5 cut(s) 115, 668, 1142, 1554, 2230
RsaNI GTAC 5 cut(s) 114, 667, 1141, 1553, 2229
RseI CAYNNNNRTG 3 cut(s) 458, 885, 2207
SacI GAGCTC 1 cut(s) 348
SapI GCTCTTC 1 cut(s) 336
SatI GCNGC 9 cut(s) 94, 561, 615, 717, 759, 846, 998, 1478, 1869
Sau3AI GATC 7 cut(s) 247, 478, 1539, 1600, 1828, 2005, 2153
Sau96I GGNCC 3 cut(s) 1289, 1497, 1944
ScaI AGTACT 1 cut(s) 1142
SchI GAGTC 5 cut(s) 1165, 1523, 1668, 1912, 2177
ScrFI CCNGG 3 cut(s) 422, 1755, 2327
SduI GDGCHC 3 cut(s) 348, 1107, 2281
SexAI ACCWGGT 1 cut(s) 2325
SfaNI GCATC 5 cut(s) 76, 1767, 1855, 1978, 2083
SfuI TTCGAA 1 cut(s) 1418
SinI GGWCC 1 cut(s) 1497
SmiMI CAYNNNNRTG 3 cut(s) 458, 885, 2207
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SpeI ACTAGT 1 cut(s) 2225
SphI GCATGC 1 cut(s) 1477
SseBI AGGCCT 2 cut(s) 179, 1840
SsiI CCGC 8 cut(s) 385, 629, 758, 845, 1395, 1457, 1526, 2146
SspI AATATT 2 cut(s) 238, 1813
SspMI CTAG 5 cut(s) 417, 947, 1034, 1817, 2226
SstI GAGCTC 1 cut(s) 348
StuI AGGCCT 2 cut(s) 179, 1840
StyD4I CCNGG 3 cut(s) 420, 1753, 2325
StyI CCWWGG 1 cut(s) 1209
TaaI ACNGT 7 cut(s) 409, 521, 658, 1123, 1145, 1940, 2307
TaiI ACGT 1 cut(s) 1651
TaqI TCGA 8 cut(s) 446, 797, 891, 1065, 1418, 1620, 2004, 2186
TatI WGTACW 5 cut(s) 113, 666, 1140, 1552, 2228
TauI GCSGC 2 cut(s) 761, 848
TfiI GAWTC 5 cut(s) 76, 263, 680, 1913, 2071
TscAI CASTG 8 cut(s) 405, 526, 874, 1240, 1360, 1855, 2155, 2242
TseFI GTSAC 4 cut(s) 686, 1117, 1606, 2237
TseI GCWGC 7 cut(s) 93, 560, 614, 716, 997, 1477, 1868
Tsp45I GTSAC 4 cut(s) 686, 1117, 1606, 2237
TspGWI ACGGA 2 cut(s) 754, 911
TspRI CASTG 8 cut(s) 405, 526, 874, 1240, 1360, 1855, 2155, 2242
Van91I CCANNNNNTGG 1 cut(s) 20
Vha464I CTTAAG 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 1497
XagI CCTNNNNNAGG 1 cut(s) 2268
XapI RAATTY 4 cut(s) 374, 442, 1698, 2101
XceI RCATGY 3 cut(s) 385, 398, 1477
XmnI GAANNNNTTC 3 cut(s) 1375, 1812, 2192
XspI CTAG 5 cut(s) 417, 947, 1034, 1817, 2226
ZrmI AGTACT 1 cut(s) 1142
Zsp2I ATGCAT 2 cut(s) 608, 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.