MD09G1288200.v1.1

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009135
Physical Location & Seq
Forward (+)
36793937 .. 36800182
6246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1288200.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGGTTGTGCTTGCTGCTTCCATTATAAGCAAGTCTGGGAAAGTGCTTGTATCTAGACAATTTGTGGATATGTCTCGTATAAGAATTGAAGGTCTTCTTGCGGCTTTCCCTAAGTTGGTTGGCACTGGCAAACAACATACTTATGTCGAGACAGAAAATGTGCGCTATGTTTACCAGCCAATAGAAGCTTTGTTCTTGGTACTTGTTACGAACAAGCAGAGCAATATACTGGAAGATTTGGAGACTTTGCGGCTACTCTCTAAACTTGCACCTGAATACTCTTTGTCCCTAGATGAAGAGGGTGTATGCAAGACAGCTTTTGAGTTAATTTTTGCTTTTGATGAAGTCATCTCTCTTGGGCACAAGGAAAACGTGACTGTTGCACAGGTGAAGCAGTACTGTGAAATGGAGAGTCATGAAGAGAAACTGCACAAGCTTGTGATGCAGAGCAAGATCAATGAAACCAAGGATGTTATGAAGCGGAAGGCTAGTGAGATTGACAAAAGCAAGATTGATAGGGGCAGAGCTGATAAAGGCGGATATATGTCTTTACAGTCAATGGGATCTGGGAGAATTGAGAGTGCGTTTAGTGAGATGAGCATATCCAGTAGTGGAAGTGGCTTTGGAGGTGGTTCTGGTTTCGGATTGAGTACTGATATTGACTCATTTTCCACAAAGACCAAAGGTCGTCCACCTTCGTCTGCTACTGCTCCTCCAAAGGGTTTGGGTATGCAGCTGGGAAAATCACAAAGGACCAACCAATTTTTGGAGTCCTTGAAAGCAGAGGGAGAGGTGATTCTTGAAGATGTACAGCCAAAAGCAGGCCAGTCTAAAACAGCTGCCCCACCTCTAACTGATCCTGTCACTTTGAATGTTGAAGAGAAACTTAACGTAACCCTTAAACGTGATGGTGGATTGAGCAACTTTGATGTTCAGGGGACATTATCTCTGCAAATTCTTAACCAGGAAGATGCATATATCCAAGTCCAGATTGAAACTGGTGGAAATCCTGGCATCCTTTTCAAAACACACCCTAACATGAACAAAGAGCTATTCTCCAATAATAACATTCTAGGCCTAAAGGATACGTCTAGGCCTTTTCCTACTGGTCAAACTGGTGATGCAGGAGGTGTTGGTCTTTTGAGATGGAGAATGCAGACTTCAGATGAGTCAATGGTGCCCCTAACAATCAACTGTTGGCCCACTGTTTCTGGGAATGAAACTTATGTCAACATTGAGTACGAGGCTTCTGAGATGTTTGATCTGAGAAATGTTGTTATCTCGGTACCTCTTCCAGCTGCGAGAGAGGCTCCACGAGTAAACCAGATTGAAGGTGAATGGAGATATGATGCCAGGAATTCAACGTTGGAGTGGTCCATACTTCTTGTAGATAACTCGAACCGCAGTGGGTCATTGGAGTTTGTTGTTCCCCCAGCAGACCCATCTGTATTTTTCCCCATTTCTGTGTGCTTCTCTGCCACCGAGACATTCAGTGACCTGAAGGTTTTGAACATTATACCTCTGCAAGGTACAGCCGCTCCCAAGTTTTCTCAAAGAACGCAGTTGATCACGGAGAATTACCTCCAGAAGCAAATATAG

Protein Analysis

533

Amino Acids

58.49

Weight (kDa)

5.41

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 283 - 506 2.3e-41 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
Acc65I GGTACC 1 cut(s) 1285
AccB1I GGYRCC 2 cut(s) 1177, 1285
AccB7I CCANNNNNTGG 1 cut(s) 766
AccBSI CCGCTC 1 cut(s) 1538
AciI CCGC 6 cut(s) 101, 250, 481, 537, 1402, 1536
AclI AACGTT 1 cut(s) 1364
AclWI GGATC 2 cut(s) 571, 851
AcsI RAATTY 2 cut(s) 954, 1357
AcuI CTGAAG 2 cut(s) 1146, 1520
AfaI GTAC 7 cut(s) 201, 398, 652, 810, 1241, 1287, 1531
AfiI CCNNNNNNNGG 5 cut(s) 115, 719, 766, 821, 1526
AhdI GACNNNNNGTC 1 cut(s) 684
AjnI CCWGG 3 cut(s) 963, 1009, 1352
AjuI GAANNNNNNNTTGG 2 cut(s) 1349, 1381
AluBI AGCT 8 cut(s) 188, 317, 436, 527, 736, 839, 1051, 1298
AluI AGCT 8 cut(s) 188, 317, 436, 527, 736, 839, 1051, 1298
Alw26I GTCTC 4 cut(s) 78, 143, 236, 1478
AlwI GGATC 2 cut(s) 571, 851
AoxI GGCC 4 cut(s) 823, 1075, 1094, 1199
ApeKI GCWGC 4 cut(s) 14, 733, 839, 1298
ApoI RAATTY 2 cut(s) 954, 1357
Asp700I GAANNNNTTC 1 cut(s) 93
Asp718I GGTACC 1 cut(s) 1285
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 3 cut(s) 753, 1200, 1374
AsuHPI GGTGA 4 cut(s) 400, 805, 1130, 1346
AvaII GGWCC 2 cut(s) 753, 1374
BaeGI GKGCMC 2 cut(s) 363, 1182
BanI GGYRCC 2 cut(s) 1177, 1285
BauI CACGAG 1 cut(s) 1314
BbsI GAAGAC 1 cut(s) 86
BbvI GCAGC 3 cut(s) 745, 826, 1285
BccI CCATC 3 cut(s) 902, 1140, 1450
BciT130I CCWGG 3 cut(s) 965, 1011, 1354
BciVI GTATCC 1 cut(s) 1078
BclI TGATCA 1 cut(s) 1566
BcoDI GTCTC 4 cut(s) 78, 143, 236, 1478
BfaI CTAG 5 cut(s) 54, 290, 489, 1073, 1092
BfuI GTATCC 1 cut(s) 1078
BisI GCNGC 7 cut(s) 15, 102, 251, 734, 840, 1299, 1536
BlsI GCNGC 7 cut(s) 16, 103, 252, 735, 841, 1300, 1537
BmcAI AGTACT 2 cut(s) 398, 652
Bme1390I CCNGG 3 cut(s) 965, 1011, 1354
Bme18I GGWCC 2 cut(s) 753, 1374
BmeRI GACNNNNNGTC 1 cut(s) 684
BmgT120I GGNCC 3 cut(s) 753, 1200, 1374
BmiI GGNNCC 3 cut(s) 1179, 1287, 1311
BmrFI CCNGG 3 cut(s) 965, 1011, 1354
BmsI GCATC 5 cut(s) 432, 961, 1023, 1111, 1339
BpiI GAAGAC 1 cut(s) 86
BplI GAGNNNNNCTC 4 cut(s) 1040, 1072, 1294, 1326
BpmI CTGGAG 1 cut(s) 1568
BsaJI CCNNGG 1 cut(s) 465
BsaXI ACNNNNNCTCC 4 cut(s) 697, 727, 1522, 1552
Bsc4I CCNNNNNNNGG 5 cut(s) 115, 719, 766, 821, 1526
Bse1I ACTGG 7 cut(s) 130, 234, 606, 826, 1003, 1111, 1120
BseBI CCWGG 3 cut(s) 965, 1011, 1354
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 2 cut(s) 475, 1014
BseLI CCNNNNNNNGG 5 cut(s) 115, 719, 766, 821, 1526
BseMII CTCAG 2 cut(s) 1242, 1256
BseNI ACTGG 7 cut(s) 130, 234, 606, 826, 1003, 1111, 1120
BseRI GAGGAG 1 cut(s) 702
BseSI GKGCMC 2 cut(s) 363, 1182
BseXI GCAGC 3 cut(s) 745, 826, 1285
BseYI CCCAGC 2 cut(s) 736, 1432
BsgI GTGCAG 1 cut(s) 413
BshFI GGCC 4 cut(s) 825, 1077, 1096, 1201
BshNI GGYRCC 2 cut(s) 1177, 1285
BslFI GGGAC 2 cut(s) 271, 952
BslI CCNNNNNNNGG 5 cut(s) 115, 719, 766, 821, 1526
BsmAI GTCTC 4 cut(s) 78, 143, 236, 1478
BsmFI GGGAC 2 cut(s) 271, 952
BsmI GAATGC 1 cut(s) 1158
BsnI GGCC 4 cut(s) 825, 1077, 1096, 1201
Bsp1286I GDGCHC 2 cut(s) 363, 1182
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 5 cut(s) 453, 563, 856, 1261, 1566
BspACI CCGC 6 cut(s) 101, 250, 481, 537, 1402, 1536
BspANI GGCC 4 cut(s) 825, 1077, 1096, 1201
BspCNI CTCAG 2 cut(s) 1243, 1257
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 1179, 1287, 1311
BspPI GGATC 2 cut(s) 571, 851
BspT107I GGYRCC 2 cut(s) 1177, 1285
BsrBI CCGCTC 1 cut(s) 1538
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 7 cut(s) 130, 234, 606, 826, 1003, 1111, 1120
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 5 cut(s) 453, 563, 856, 1261, 1566
BssSI CACGAG 1 cut(s) 1314
BssT1I CCWWGG 1 cut(s) 465
Bst2BI CACGAG 1 cut(s) 1314
Bst2UI CCWGG 3 cut(s) 965, 1011, 1354
Bst4CI ACNGT 5 cut(s) 379, 401, 555, 1196, 1207
Bst6I CTCTTC 4 cut(s) 291, 414, 873, 1296
BstAUI TGTACA 1 cut(s) 808
BstC8I GCNNGC 2 cut(s) 12, 823
BstDEI CTNAG 3 cut(s) 111, 1251, 1265
BstENI CCTNNNNNAGG 1 cut(s) 1524
BstF5I GGATG 2 cut(s) 475, 1014
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 5 cut(s) 456, 566, 859, 1264, 1569
BstMAI GTCTC 4 cut(s) 78, 143, 236, 1478
BstMBI GATC 5 cut(s) 453, 563, 856, 1261, 1566
BstMWI GCNNNNNNNGC 2 cut(s) 442, 1307
BstNI CCWGG 3 cut(s) 965, 1011, 1354
BstSCI CCNGG 3 cut(s) 963, 1009, 1352
BstSLI GKGCMC 2 cut(s) 363, 1182
BstV1I GCAGC 3 cut(s) 745, 826, 1285
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 1 cut(s) 563
BstYI RGATCY 1 cut(s) 563
BsuI GTATCC 1 cut(s) 1078
BsuRI GGCC 4 cut(s) 825, 1077, 1096, 1201
BtsCI GGATG 2 cut(s) 475, 1014
BtsI GCAGTG 1 cut(s) 1411
BtsIMutI CAGTG 4 cut(s) 123, 1203, 1411, 1498
Cac8I GCNNGC 2 cut(s) 12, 823
CciI TCATGA 1 cut(s) 415
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 3 cut(s) 753, 1200, 1374
Csp6I GTAC 7 cut(s) 200, 397, 651, 809, 1240, 1286, 1530
CviAII CATG 2 cut(s) 416, 1039
CviQI GTAC 7 cut(s) 200, 397, 651, 809, 1240, 1286, 1530
DdeI CTNAG 3 cut(s) 111, 1251, 1265
DpnI GATC 5 cut(s) 455, 565, 858, 1263, 1568
DpnII GATC 5 cut(s) 453, 563, 856, 1261, 1566
DriI GACNNNNNGTC 1 cut(s) 684
Eam1104I CTCTTC 4 cut(s) 291, 414, 873, 1296
Eam1105I GACNNNNNGTC 1 cut(s) 684
EarI CTCTTC 4 cut(s) 291, 414, 873, 1296
EciI GGCGGA 1 cut(s) 552
Eco130I CCWWGG 1 cut(s) 465
Eco147I AGGCCT 2 cut(s) 1077, 1096
Eco47I GGWCC 2 cut(s) 753, 1374
Eco57I CTGAAG 2 cut(s) 1146, 1520
EcoNI CCTNNNNNAGG 1 cut(s) 1524
EcoRI GAATTC 1 cut(s) 1357
EcoRII CCWGG 3 cut(s) 963, 1009, 1352
EcoT14I CCWWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 976
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 2 cut(s) 419, 1042
FalI AAGNNNNNCTT 4 cut(s) 81, 113, 870, 902
FaqI GGGAC 2 cut(s) 271, 952
FatI CATG 2 cut(s) 415, 1038
FbaI TGATCA 1 cut(s) 1566
Fnu4HI GCNGC 7 cut(s) 15, 102, 251, 734, 840, 1299, 1536
FokI GGATG 2 cut(s) 482, 1001
Fsp4HI GCNGC 7 cut(s) 15, 102, 251, 734, 840, 1299, 1536
FspBI CTAG 5 cut(s) 54, 290, 489, 1073, 1092
GlaI GCGC 1 cut(s) 164
GluI GCNGC 7 cut(s) 15, 102, 251, 734, 840, 1299, 1536
GsaI CCCAGC 2 cut(s) 740, 1436
GsuI CTGGAG 1 cut(s) 1568
HaeIII GGCC 4 cut(s) 825, 1077, 1096, 1201
HhaI GCGC 1 cut(s) 165
Hin1II CATG 2 cut(s) 419, 1042
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HindIII AAGCTT 2 cut(s) 186, 434
HinfI GANTC 5 cut(s) 412, 662, 770, 796, 1169
HphI GGTGA 4 cut(s) 400, 805, 1130, 1346
Hpy166II GTNNAC 4 cut(s) 172, 692, 1231, 1321
Hpy188I TCNGA 4 cut(s) 644, 1165, 1252, 1266
Hpy188III TCNNGA 6 cut(s) 54, 148, 416, 800, 988, 1585
Hpy8I GTNNAC 4 cut(s) 172, 692, 1231, 1321
HpyAV CCTTC 5 cut(s) 83, 478, 705, 1325, 1495
HpyCH4III ACNGT 5 cut(s) 379, 401, 555, 1196, 1207
HpyCH4IV ACGT 5 cut(s) 372, 891, 904, 1088, 1364
HpyF10VI GCNNNNNNNGC 2 cut(s) 442, 1307
HpyF3I CTNAG 3 cut(s) 111, 1251, 1265
HpySE526I ACGT 5 cut(s) 372, 891, 904, 1088, 1364
Hsp92II CATG 2 cut(s) 419, 1042
HspAI GCGC 1 cut(s) 163
KpnI GGTACC 1 cut(s) 1289
Ksp22I TGATCA 1 cut(s) 1566
Kzo9I GATC 5 cut(s) 453, 563, 856, 1261, 1566
LmnI GCTCC 3 cut(s) 715, 1315, 1543
Lsp1109I GCAGC 3 cut(s) 745, 826, 1285
LweI GCATC 5 cut(s) 432, 961, 1023, 1111, 1339
MaeI CTAG 5 cut(s) 54, 290, 489, 1073, 1092
MaeII ACGT 5 cut(s) 372, 891, 904, 1088, 1364
MaeIII GTNAC 5 cut(s) 205, 373, 862, 892, 1493
MalI GATC 5 cut(s) 455, 565, 858, 1263, 1568
MbiI CCGCTC 1 cut(s) 1538
MboI GATC 5 cut(s) 453, 563, 856, 1261, 1566
MboII GAAGA 8 cut(s) 86, 245, 308, 431, 815, 890, 980, 1283
MflI RGATCY 1 cut(s) 563
MhlI GDGCHC 2 cut(s) 363, 1182
MluCI AATT 8 cut(s) 59, 84, 327, 573, 761, 954, 1357, 1576
MlyI GAGTC 4 cut(s) 421, 656, 779, 1178
MmeI TCCRAC 1 cut(s) 1347
Mph1103I ATGCAT 1 cut(s) 976
MroXI GAANNNNTTC 1 cut(s) 93
MseI TTAA 4 cut(s) 326, 888, 900, 960
MslI CAYNNNNRTG 2 cut(s) 141, 1463
MspA1I CMGCKG 3 cut(s) 736, 839, 1298
MspR9I CCNGG 3 cut(s) 965, 1011, 1354
Mva1269I GAATGC 1 cut(s) 1158
MvaI CCWGG 3 cut(s) 965, 1011, 1354
MwoI GCNNNNNNNGC 2 cut(s) 442, 1307
NdeII GATC 5 cut(s) 453, 563, 856, 1261, 1566
NlaIII CATG 2 cut(s) 419, 1042
NlaIV GGNNCC 3 cut(s) 1179, 1287, 1311
NmuCI GTSAC 3 cut(s) 373, 862, 1493
NsiI ATGCAT 1 cut(s) 976
PagI TCATGA 1 cut(s) 415
PceI AGGCCT 2 cut(s) 1077, 1096
PctI GAATGC 1 cut(s) 1158
PdmI GAANNNNTTC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 796
PflMI CCANNNNNTGG 1 cut(s) 766
PkrI GCNGC 7 cut(s) 16, 103, 252, 735, 841, 1300, 1537
PleI GAGTC 4 cut(s) 420, 656, 778, 1177
PpsI GAGTC 4 cut(s) 420, 656, 778, 1177
PsiI TTATAA 1 cut(s) 26
Psp1406I AACGTT 1 cut(s) 1364
Psp6I CCWGG 3 cut(s) 963, 1009, 1352
PspFI CCCAGC 2 cut(s) 736, 1432
PspGI CCWGG 3 cut(s) 963, 1009, 1352
PspN4I GGNNCC 3 cut(s) 1179, 1287, 1311
PspPI GGNCC 3 cut(s) 753, 1200, 1374
PsuI RGATCY 1 cut(s) 563
PvuII CAGCTG 3 cut(s) 736, 839, 1298
RsaI GTAC 7 cut(s) 201, 398, 652, 810, 1241, 1287, 1531
RsaNI GTAC 7 cut(s) 200, 397, 651, 809, 1240, 1286, 1530
RseI CAYNNNNRTG 2 cut(s) 141, 1463
SaqAI TTAA 4 cut(s) 326, 888, 900, 960
SatI GCNGC 7 cut(s) 15, 102, 251, 734, 840, 1299, 1536
Sau3AI GATC 5 cut(s) 453, 563, 856, 1261, 1566
Sau96I GGNCC 3 cut(s) 753, 1200, 1374
ScaI AGTACT 2 cut(s) 398, 652
SchI GAGTC 4 cut(s) 421, 656, 779, 1178
ScrFI CCNGG 3 cut(s) 965, 1011, 1354
SduI GDGCHC 2 cut(s) 363, 1182
SfaNI GCATC 5 cut(s) 432, 961, 1023, 1111, 1339
SinI GGWCC 2 cut(s) 753, 1374
SmiMI CAYNNNNRTG 2 cut(s) 141, 1463
Sse9I AATT 8 cut(s) 59, 84, 327, 573, 761, 954, 1357, 1576
SseBI AGGCCT 2 cut(s) 1077, 1096
SsiI CCGC 6 cut(s) 101, 250, 481, 537, 1402, 1536
SspMI CTAG 5 cut(s) 54, 290, 489, 1073, 1092
StuI AGGCCT 2 cut(s) 1077, 1096
StyD4I CCNGG 3 cut(s) 963, 1009, 1352
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 5 cut(s) 379, 401, 555, 1196, 1207
TaiI ACGT 5 cut(s) 375, 894, 907, 1091, 1367
TaqI TCGA 2 cut(s) 147, 1397
TasI AATT 8 cut(s) 59, 84, 327, 573, 761, 954, 1357, 1576
TatI WGTACW 3 cut(s) 396, 650, 808
TauI GCSGC 3 cut(s) 104, 253, 1538
TfiI GAWTC 1 cut(s) 796
Tru1I TTAA 4 cut(s) 326, 888, 900, 960
Tru9I TTAA 4 cut(s) 326, 888, 900, 960
TscAI CASTG 4 cut(s) 130, 1210, 1411, 1498
TseFI GTSAC 3 cut(s) 373, 862, 1493
TseI GCWGC 4 cut(s) 14, 733, 839, 1298
Tsp45I GTSAC 3 cut(s) 373, 862, 1493
TspDTI ATGAA 7 cut(s) 309, 357, 432, 474, 491, 1055, 1233
TspGWI ACGGA 1 cut(s) 1586
TspRI CASTG 4 cut(s) 130, 1210, 1411, 1498
Van91I CCANNNNNTGG 1 cut(s) 766
VpaK11BI GGWCC 2 cut(s) 753, 1374
XagI CCTNNNNNAGG 1 cut(s) 1524
XapI RAATTY 2 cut(s) 954, 1357
XbaI TCTAGA 1 cut(s) 53
XcmI CCANNNNNNNNNTGG 2 cut(s) 763, 995
XmnI GAANNNNTTC 1 cut(s) 93
XspI CTAG 5 cut(s) 54, 290, 489, 1073, 1092
ZrmI AGTACT 2 cut(s) 398, 652
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.