Rh7CG315900

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0009135
Physical Location & Seq
Reverse (-)
33690814 .. 33715684
24871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG315900.1

Sequence Viewer

Length: 2283 bp
ATGAGCTGTCTCCCAAAGCTTGGAAATGATTTGCAAAAGTGCCGAGCCAAAGGCTTGATGTCAGAGCATCAGAGGATTCAAGTGATAATTTGCTGCCAGTTGCTCCTCTTCAGTACAGATAGCTTAAAAGACAGCTTGAAGAAAGTTGAAGATAAGTTAATTATTGCCAATTCTGAGGCCTTTACTCTGAGCGAAAAGGTAAGTTCGCTTGAAGATGAAATAAGTGAAATGGGAAATATTATTACAGATCTTAAAGAGAAAGAATCAAAAGCTGAAGGTAGGGCTTGGAATGCAGAAGCCAAATGCAATTTATTAACAGAGACCAGCGCTGGACTTAATGAAGAGCTCAGACTTCTTAAGACGCAGTTAACAGAATTTGACATGCGGTTGCAACATGCAGTGGCAACTGTTGGAGCTAGCCAGGAGAAGCAAATCATGTTGAATTCGATTATCATACATATGGAAAATGTGATAAAGGATCTAAAGCAGAAGGTTTCAAACGCTGAAAGTCGGGCTGACAGTGTAGAGGAAAAGTGTATAATAATATCAAAATCCAATGCAGCACTAAATGATAAAGTAAGCTTTCTGACGGGTAGATTGAAATGCATGGAGGCAGCTTTGTATCAAGCGGAGGAAGCAAAAATAGCTATTGCAACTGTTATTGAGTACATTGCAACTGAATCTGGTCACATATTTCAAGCAGTTTTCATTGGAGCTGCAATTTTCCCGATAGTGCTTGTTTCGAGGCAGTTTATGGATATGTCTCGTATAAGGATTGAGGGTCTTCTTGCGGCTTTCCCTAAGTTGGTAGGCACTGGAAAACAACATACATATGTCGAGACGGAAAATGTGCGCTATGTTTACCAGCCAATAGAAGCTTTGTATTTGCTTCTAGTTACAAACAAGCAGAGCAATATCTTAGAAGATTTGGAGACTTTGAGGCTGCTCTCTAAGCTTGTGCCTGAATATGCATTGTCCCTAGATGAAGAGGGTATATGCAGGACAGCTTTCGAGTTGATTTTTGCTTTTGATGAAGTAATCTCTCTTGGGCACAAGGAGAATGTGACTGTTGCACAGGTGAAGCAGTACTGTGAGATGGAGAGTCATGAAGAGAAGCTTCACAAGCTCGTAATGCAGAGCAAGATAAATGAGACCAAGGATGTTATGAAGCGTAAAGCCAGTGAGATTGACAAAAGCAAGATTGATAGGAGCAGAGGTGAAAAGGGAGGATTTGGGCCTTTGCAGTCAATGGGTTCTGGAAGAATTGAGAGTGCCTTTAGTGAGATGAGCTTATCCAGCACTGGAAGTGGTTTTGGAAGTGGTTCTGGTTTGGGAGCGACCGCTGATTTTGAAACATTTTCTTCGAAGGCCAAAGGCCGTCCACCTTCATCTGCCACAGCTCCGCCAAAGGGTTTAGGCATGCAGCTTGGAAAATCACAAAGGACCAACCAATTTTTAGAGTCCTTAAAAGCGGAGGGAGAGGTGATCCTTGAAGATGTACAGCCAAAAGCAGGCCAGTCAAAATTGGTTGCCCCACCTTTAACTGATCCCGTCACTTTGAATGTCGAAGAGAAACTTAATGTAACACTTAAACGTGATGGTGGAGTCAGTAACTTTGATGTTCAAGGAACATTATCCCTGCAAATTCTTAACCAAGAAGACGGGCATATCCAAGTCCAGATTGAAACCGGTGGAAATCCTGGCATCCTTTTTAAAACTCATCCTAACATGAACAAAGAACTATTTTCTAATGAAAATATTCTAGGCTTGAAAGATCCTAATAGGCCTTTCCCCACTGGTCAAGCTGGTGATGCAGCAGGTGTTGGTCTTTTGAAATGGAGAATGCAGAGTCAAGATGAATCATTGGTGCCTCTACAAATCAACTGTTGGCCCTCTGTTTCTGGAAATGAAACCTATGTCAACATTGAGTATGATGCTTCATCAATGTTCGATCTGCGAAATGTTCTCCTTTCAGTTCCTCTTCCAGCTCTCAGAGAGGCTCCAGTTGTGAAGGAGATTCATGGTGAATGGAGATATGATGCAAGGAATTCAGTATTGGAGTGGTCTATACTTCTTATTGATAACTCAAACCGCAGTGGATCAATGGAGTTTGTTGTTCCTCCAGCAGACTCGAATGTATTCTTTCCCATTTCTGTGCGCTTCTCTGCCACTAGTACATTCAGTGACCTGAAGGTTGTGAATATCTTACCTCTGAAAGGTGGAGCCCCTCCCAAGTTCGCTCAGAGAACACAGTTGCTCACAGAGAACTACCAGGTGGTGTAA

Protein Analysis

760

Amino Acids

83.81

Weight (kDa)

5.49

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 515 - 744 9e-42 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1808
Acc36I ACCTGC 1 cut(s) 1808
AccB1I GGYRCC 1 cut(s) 1867
AccB7I CCANNNNNTGG 1 cut(s) 20
AciI CCGC 7 cut(s) 385, 629, 791, 1341, 1403, 1472, 2092
AclWI GGATC 5 cut(s) 486, 1480, 1541, 1769, 2107
AcsI RAATTY 4 cut(s) 374, 442, 1644, 2047
AcuI CTGAAG 3 cut(s) 94, 294, 2210
AfaI GTAC 5 cut(s) 115, 668, 1088, 1500, 2176
AfeI AGCGCT 1 cut(s) 328
AfiI CCNNNNNNNGG 5 cut(s) 20, 805, 1409, 1511, 2216
AflII CTTAAG 1 cut(s) 356
AgeI ACCGGT 1 cut(s) 1688
AhlI ACTAGT 1 cut(s) 2171
AjnI CCWGG 3 cut(s) 420, 1699, 2271
AjuI GAANNNNNNNTTGG 2 cut(s) 2039, 2071
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 6 cut(s) 14, 314, 768, 833, 926, 1145
AlwI GGATC 5 cut(s) 486, 1480, 1541, 1769, 2107
Aor51HI AGCGCT 1 cut(s) 328
AoxI GGCC 7 cut(s) 177, 1235, 1368, 1375, 1513, 1784, 1889
ApeKI GCWGC 7 cut(s) 93, 560, 614, 716, 943, 1423, 1814
ApoI RAATTY 4 cut(s) 374, 442, 1644, 2047
AsiGI ACCGGT 1 cut(s) 1688
Asp700I GAANNNNTTC 3 cut(s) 1321, 1758, 2138
AspLEI GCGC 3 cut(s) 329, 855, 2160
AspS9I GGNCC 3 cut(s) 1235, 1443, 1890
AsuHPI GGTGA 5 cut(s) 1090, 1229, 1495, 1820, 2036
AsuII TTCGAA 1 cut(s) 1364
AsuNHI GCTAGC 1 cut(s) 416
AvaII GGWCC 1 cut(s) 1443
BaeGI GKGCMC 1 cut(s) 1053
BanI GGYRCC 1 cut(s) 1867
BanII GRGCYC 2 cut(s) 348, 2227
BbsI GAAGAC 2 cut(s) 776, 1665
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 7 cut(s) 80, 572, 626, 703, 930, 1435, 1826
BccI CCATC 2 cut(s) 1090, 1592
BceAI ACGGC 1 cut(s) 1362
BciT130I CCWGG 3 cut(s) 422, 1701, 2273
BcoDI GTCTC 6 cut(s) 14, 314, 768, 833, 926, 1145
BcuI ACTAGT 1 cut(s) 2171
BfaI CTAG 5 cut(s) 417, 893, 980, 1763, 2172
BfoI RGCGCY 1 cut(s) 330
BfrI CTTAAG 1 cut(s) 356
BfuAI ACCTGC 1 cut(s) 1808
BglII AGATCT 1 cut(s) 247
BisI GCNGC 8 cut(s) 94, 561, 615, 717, 792, 944, 1424, 1815
BlsI GCNGC 8 cut(s) 95, 562, 616, 718, 793, 945, 1425, 1816
BmcAI AGTACT 1 cut(s) 1088
Bme1390I CCNGG 3 cut(s) 422, 1701, 2273
Bme18I GGWCC 1 cut(s) 1443
BmgT120I GGNCC 3 cut(s) 1235, 1443, 1890
BmiI GGNNCC 3 cut(s) 1869, 2001, 2224
BmrFI CCNGG 3 cut(s) 422, 1701, 2273
BmsI GCATC 5 cut(s) 76, 1713, 1801, 1924, 2029
BmtI GCTAGC 1 cut(s) 420
BpiI GAAGAC 2 cut(s) 776, 1665
BpmI CTGGAG 2 cut(s) 1986, 2106
Bpu14I TTCGAA 1 cut(s) 1364
BsaI GGTCTC 2 cut(s) 314, 1145
BsaJI CCNNGG 1 cut(s) 1155
BsaWI WCCGGW 1 cut(s) 1688
BsaXI ACNNNNNCTCC 2 cut(s) 1201, 1231
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 805, 1409, 1511, 2216
Bse118I RCCGGY 1 cut(s) 1688
Bse1I ACTGG 7 cut(s) 97, 820, 1179, 1306, 1516, 1801, 2003
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 3 cut(s) 422, 1701, 2273
BseDI CCNNGG 1 cut(s) 1155
BseGI GGATG 3 cut(s) 1165, 1704, 1720
BseLI CCNNNNNNNGG 5 cut(s) 20, 805, 1409, 1511, 2216
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 5 cut(s) 165, 179, 361, 2005, 2255
BseNI ACTGG 7 cut(s) 97, 820, 1179, 1306, 1516, 1801, 2003
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 1053
BseXI GCAGC 7 cut(s) 80, 572, 626, 703, 930, 1435, 1826
Bsh1285I CGRYCG 1 cut(s) 1341
BshFI GGCC 7 cut(s) 179, 1237, 1370, 1377, 1515, 1786, 1891
BshNI GGYRCC 1 cut(s) 1867
BshTI ACCGGT 1 cut(s) 1688
BsiEI CGRYCG 1 cut(s) 1341
BsiHKAI GWGCWC 1 cut(s) 348
BsiSI CCGG 1 cut(s) 1689
BslFI GGGAC 1 cut(s) 961
BslI CCNNNNNNNGG 5 cut(s) 20, 805, 1409, 1511, 2216
BsmAI GTCTC 6 cut(s) 14, 314, 768, 833, 926, 1145
BsmBI CGTCTC 1 cut(s) 833
BsmFI GGGAC 1 cut(s) 961
BsmI GAATGC 2 cut(s) 295, 1848
BsnI GGCC 7 cut(s) 179, 1237, 1370, 1377, 1515, 1786, 1891
Bso31I GGTCTC 2 cut(s) 314, 1145
Bsp119I TTCGAA 1 cut(s) 1364
Bsp1286I GDGCHC 3 cut(s) 348, 1053, 2227
Bsp1407I TGTACA 1 cut(s) 1498
Bsp143I GATC 7 cut(s) 247, 478, 1485, 1546, 1774, 1951, 2099
BspACI CCGC 7 cut(s) 385, 629, 791, 1341, 1403, 1472, 2092
BspANI GGCC 7 cut(s) 179, 1237, 1370, 1377, 1515, 1786, 1891
BspCNI CTCAG 5 cut(s) 166, 180, 360, 2004, 2254
BspHI TCATGA 1 cut(s) 1105
BspLI GGNNCC 3 cut(s) 1869, 2001, 2224
BspMI ACCTGC 1 cut(s) 1808
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 5 cut(s) 486, 1480, 1541, 1769, 2107
BspQI GCTCTTC 1 cut(s) 336
BspT104I TTCGAA 1 cut(s) 1364
BspT107I GGYRCC 1 cut(s) 1867
BspTI CTTAAG 1 cut(s) 356
BspTNI GGTCTC 2 cut(s) 314, 1145
BsrDI GCAATG 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 1688
BsrGI TGTACA 1 cut(s) 1498
BsrI ACTGG 7 cut(s) 97, 820, 1179, 1306, 1516, 1801, 2003
BssAI RCCGGY 1 cut(s) 1688
BssECI CCNNGG 1 cut(s) 1155
BssMI GATC 7 cut(s) 247, 478, 1485, 1546, 1774, 1951, 2099
BssT1I CCWWGG 1 cut(s) 1155
Bst2UI CCWGG 3 cut(s) 422, 1701, 2273
Bst4CI ACNGT 7 cut(s) 409, 521, 658, 1069, 1091, 1886, 2253
Bst6I CTCTTC 6 cut(s) 113, 336, 981, 1104, 1563, 1986
BstAFI CTTAAG 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 1498
BstBI TTCGAA 1 cut(s) 1364
BstC8I GCNNGC 3 cut(s) 418, 1421, 1513
BstDEI CTNAG 8 cut(s) 174, 188, 347, 801, 919, 951, 1991, 2241
BstENI CCTNNNNNAGG 1 cut(s) 2214
BstF5I GGATG 3 cut(s) 1165, 1704, 1720
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 3 cut(s) 329, 855, 2160
BstKTI GATC 7 cut(s) 250, 481, 1488, 1549, 1777, 1954, 2102
BstMAI GTCTC 6 cut(s) 14, 314, 768, 833, 926, 1145
BstMBI GATC 7 cut(s) 247, 478, 1485, 1546, 1774, 1951, 2099
BstMCI CGRYCG 1 cut(s) 1341
BstMWI GCNNNNNNNGC 8 cut(s) 290, 635, 644, 952, 1123, 1132, 1296, 1811
BstNI CCWGG 3 cut(s) 422, 1701, 2273
BstNSI RCATGY 3 cut(s) 385, 398, 1423
BstSCI CCNGG 3 cut(s) 420, 1699, 2271
BstSLI GKGCMC 1 cut(s) 1053
BstV1I GCAGC 7 cut(s) 80, 572, 626, 703, 930, 1435, 1826
BstV2I GAAGAC 2 cut(s) 776, 1665
BstX2I RGATCY 3 cut(s) 247, 478, 1774
BstYI RGATCY 3 cut(s) 247, 478, 1774
BsuRI GGCC 7 cut(s) 179, 1237, 1370, 1377, 1515, 1786, 1891
BtsCI GGATG 3 cut(s) 1165, 1704, 1720
BtsI GCAGTG 2 cut(s) 405, 2101
BtsIMutI CAGTG 8 cut(s) 405, 526, 813, 1186, 1299, 1794, 2101, 2188
BveI ACCTGC 1 cut(s) 1808
Cac8I GCNNGC 3 cut(s) 418, 1421, 1513
CciI TCATGA 1 cut(s) 1105
CfoI GCGC 3 cut(s) 329, 855, 2160
Cfr10I RCCGGY 1 cut(s) 1688
Cfr13I GGNCC 3 cut(s) 1235, 1443, 1890
CseI GACGC 1 cut(s) 370
CsiI ACCWGGT 1 cut(s) 2271
Csp6I GTAC 5 cut(s) 114, 667, 1087, 1499, 2175
CspAI ACCGGT 1 cut(s) 1688
CviAII CATG 8 cut(s) 382, 395, 436, 607, 1106, 1420, 1729, 2021
CviQI GTAC 5 cut(s) 114, 667, 1087, 1499, 2175
DdeI CTNAG 8 cut(s) 174, 188, 347, 801, 919, 951, 1991, 2241
DpnI GATC 7 cut(s) 249, 480, 1487, 1548, 1776, 1953, 2101
DpnII GATC 7 cut(s) 247, 478, 1485, 1546, 1774, 1951, 2099
DraI TTTAAA 1 cut(s) 1714
Eam1104I CTCTTC 6 cut(s) 113, 336, 981, 1104, 1563, 1986
EarI CTCTTC 6 cut(s) 113, 336, 981, 1104, 1563, 1986
EciI GGCGGA 1 cut(s) 1392
Ecl136II GAGCTC 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 1155
Eco147I AGGCCT 2 cut(s) 179, 1786
Eco24I GRGCYC 2 cut(s) 348, 2227
Eco31I GGTCTC 2 cut(s) 314, 1145
Eco47I GGWCC 1 cut(s) 1443
Eco47III AGCGCT 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 346
Eco57I CTGAAG 3 cut(s) 94, 294, 2210
EcoICRI GAGCTC 1 cut(s) 346
EcoNI CCTNNNNNAGG 1 cut(s) 2214
EcoRI GAATTC 2 cut(s) 442, 2047
EcoRII CCWGG 3 cut(s) 420, 1699, 2271
EcoT14I CCWWGG 1 cut(s) 1155
EcoT22I ATGCAT 2 cut(s) 608, 973
EcoT38I GRGCYC 2 cut(s) 348, 2227
ErhI CCWWGG 1 cut(s) 1155
Esp3I CGTCTC 1 cut(s) 833
FaeI CATG 8 cut(s) 385, 398, 439, 610, 1109, 1423, 1732, 2024
FalI AAGNNNNNCTT 4 cut(s) 1101, 1133, 1560, 1592
FaqI GGGAC 1 cut(s) 961
FatI CATG 8 cut(s) 381, 394, 435, 606, 1105, 1419, 1728, 2020
FauNDI CATATG 2 cut(s) 459, 832
Fnu4HI GCNGC 8 cut(s) 94, 561, 615, 717, 792, 944, 1424, 1815
FokI GGATG 3 cut(s) 1172, 1691, 1707
FriOI GRGCYC 2 cut(s) 348, 2227
Fsp4HI GCNGC 8 cut(s) 94, 561, 615, 717, 792, 944, 1424, 1815
FspBI CTAG 5 cut(s) 417, 893, 980, 1763, 2172
GlaI GCGC 3 cut(s) 328, 854, 2159
GluI GCNGC 8 cut(s) 94, 561, 615, 717, 792, 944, 1424, 1815
GsuI CTGGAG 2 cut(s) 1986, 2106
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 7 cut(s) 179, 1237, 1370, 1377, 1515, 1786, 1891
HapII CCGG 1 cut(s) 1689
HgaI GACGC 1 cut(s) 370
HhaI GCGC 3 cut(s) 329, 855, 2160
Hin1II CATG 8 cut(s) 385, 398, 439, 610, 1109, 1423, 1732, 2024
Hin6I GCGC 3 cut(s) 327, 853, 2158
HinP1I GCGC 3 cut(s) 327, 853, 2158
HincII GTYRAC 2 cut(s) 369, 1921
HindII GTYRAC 2 cut(s) 369, 1921
HindIII AAGCTT 5 cut(s) 17, 580, 876, 953, 1115
HpaI GTTAAC 1 cut(s) 369
HpaII CCGG 1 cut(s) 1689
HphI GGTGA 5 cut(s) 1090, 1229, 1495, 1820, 2036
Hpy166II GTNNAC 4 cut(s) 369, 862, 1382, 1921
Hpy188I TCNGA 9 cut(s) 64, 72, 175, 189, 350, 588, 1994, 2214, 2244
Hpy188III TCNNGA 7 cut(s) 727, 838, 1106, 1257, 1678, 1853, 1902
Hpy8I GTNNAC 4 cut(s) 369, 862, 1382, 1921
HpyAV CCTTC 6 cut(s) 269, 484, 1360, 1395, 2005, 2185
HpyCH4III ACNGT 7 cut(s) 409, 521, 658, 1069, 1091, 1886, 2253
HpyCH4IV ACGT 1 cut(s) 1594
HpyF10VI GCNNNNNNNGC 8 cut(s) 290, 635, 644, 952, 1123, 1132, 1296, 1811
HpyF3I CTNAG 8 cut(s) 174, 188, 347, 801, 919, 951, 1991, 2241
HpySE526I ACGT 1 cut(s) 1594
Hsp92II CATG 8 cut(s) 385, 398, 439, 610, 1109, 1423, 1732, 2024
HspAI GCGC 3 cut(s) 327, 853, 2158
KspAI GTTAAC 1 cut(s) 369
Kzo9I GATC 7 cut(s) 247, 478, 1485, 1546, 1774, 1951, 2099
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 8 cut(s) 108, 413, 713, 1209, 1334, 1405, 2005, 2222
Lsp1109I GCAGC 7 cut(s) 80, 572, 626, 703, 930, 1435, 1826
LweI GCATC 5 cut(s) 76, 1713, 1801, 1924, 2029
MabI ACCWGGT 1 cut(s) 2271
MaeI CTAG 5 cut(s) 417, 893, 980, 1763, 2172
MaeII ACGT 1 cut(s) 1594
MaeIII GTNAC 7 cut(s) 686, 895, 1063, 1552, 1582, 1610, 2183
MalI GATC 7 cut(s) 249, 480, 1487, 1548, 1776, 1953, 2101
MboI GATC 7 cut(s) 247, 478, 1485, 1546, 1774, 1951, 2099
MflI RGATCY 3 cut(s) 247, 478, 1774
MhlI GDGCHC 3 cut(s) 348, 1053, 2227
MlyI GAGTC 5 cut(s) 1111, 1469, 1614, 1858, 2123
MmeI TCCRAC 1 cut(s) 391
Mph1103I ATGCAT 2 cut(s) 608, 973
MroXI GAANNNNTTC 3 cut(s) 1321, 1758, 2138
MslI CAYNNNNRTG 3 cut(s) 458, 831, 2153
MspA1I CMGCKG 1 cut(s) 1343
MspCI CTTAAG 1 cut(s) 356
MspI CCGG 1 cut(s) 1689
MspR9I CCNGG 3 cut(s) 422, 1701, 2273
Mva1269I GAATGC 2 cut(s) 295, 1848
MvaI CCWGG 3 cut(s) 422, 1701, 2273
MwoI GCNNNNNNNGC 8 cut(s) 290, 635, 644, 952, 1123, 1132, 1296, 1811
NdeI CATATG 2 cut(s) 459, 832
NdeII GATC 7 cut(s) 247, 478, 1485, 1546, 1774, 1951, 2099
NheI GCTAGC 1 cut(s) 416
NlaIII CATG 8 cut(s) 385, 398, 439, 610, 1109, 1423, 1732, 2024
NlaIV GGNNCC 3 cut(s) 1869, 2001, 2224
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 4 cut(s) 686, 1063, 1552, 2183
NsiI ATGCAT 2 cut(s) 608, 973
NspI RCATGY 3 cut(s) 385, 398, 1423
NspV TTCGAA 1 cut(s) 1364
PaeI GCATGC 1 cut(s) 1423
PagI TCATGA 1 cut(s) 1105
PaqCI CACCTGC 1 cut(s) 1808
PceI AGGCCT 2 cut(s) 179, 1786
PciSI GCTCTTC 1 cut(s) 336
PctI GAATGC 2 cut(s) 295, 1848
PdmI GAANNNNTTC 3 cut(s) 1321, 1758, 2138
PfeI GAWTC 5 cut(s) 76, 263, 680, 1859, 2017
PflMI CCANNNNNTGG 1 cut(s) 20
PinAI ACCGGT 1 cut(s) 1688
PkrI GCNGC 8 cut(s) 95, 562, 616, 718, 793, 945, 1425, 1816
PleI GAGTC 5 cut(s) 1110, 1468, 1613, 1857, 2123
PpsI GAGTC 5 cut(s) 1110, 1468, 1613, 1857, 2123
Psp124BI GAGCTC 1 cut(s) 348
Psp6I CCWGG 3 cut(s) 420, 1699, 2271
PspGI CCWGG 3 cut(s) 420, 1699, 2271
PspN4I GGNNCC 3 cut(s) 1869, 2001, 2224
PspPI GGNCC 3 cut(s) 1235, 1443, 1890
PsuI RGATCY 3 cut(s) 247, 478, 1774
RsaI GTAC 5 cut(s) 115, 668, 1088, 1500, 2176
RsaNI GTAC 5 cut(s) 114, 667, 1087, 1499, 2175
RseI CAYNNNNRTG 3 cut(s) 458, 831, 2153
SacI GAGCTC 1 cut(s) 348
SapI GCTCTTC 1 cut(s) 336
SatI GCNGC 8 cut(s) 94, 561, 615, 717, 792, 944, 1424, 1815
Sau3AI GATC 7 cut(s) 247, 478, 1485, 1546, 1774, 1951, 2099
Sau96I GGNCC 3 cut(s) 1235, 1443, 1890
ScaI AGTACT 1 cut(s) 1088
SchI GAGTC 5 cut(s) 1111, 1469, 1614, 1858, 2123
ScrFI CCNGG 3 cut(s) 422, 1701, 2273
SduI GDGCHC 3 cut(s) 348, 1053, 2227
SexAI ACCWGGT 1 cut(s) 2271
SfaNI GCATC 5 cut(s) 76, 1713, 1801, 1924, 2029
SfuI TTCGAA 1 cut(s) 1364
SinI GGWCC 1 cut(s) 1443
SmiMI CAYNNNNRTG 3 cut(s) 458, 831, 2153
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SpeI ACTAGT 1 cut(s) 2171
SphI GCATGC 1 cut(s) 1423
SseBI AGGCCT 2 cut(s) 179, 1786
SsiI CCGC 7 cut(s) 385, 629, 791, 1341, 1403, 1472, 2092
SspI AATATT 2 cut(s) 238, 1759
SspMI CTAG 5 cut(s) 417, 893, 980, 1763, 2172
SstI GAGCTC 1 cut(s) 348
StuI AGGCCT 2 cut(s) 179, 1786
StyD4I CCNGG 3 cut(s) 420, 1699, 2271
StyI CCWWGG 1 cut(s) 1155
TaaI ACNGT 7 cut(s) 409, 521, 658, 1069, 1091, 1886, 2253
TaiI ACGT 1 cut(s) 1597
TaqI TCGA 8 cut(s) 446, 743, 837, 1011, 1364, 1566, 1950, 2132
TatI WGTACW 5 cut(s) 113, 666, 1086, 1498, 2174
TauI GCSGC 1 cut(s) 794
TfiI GAWTC 5 cut(s) 76, 263, 680, 1859, 2017
TscAI CASTG 8 cut(s) 405, 526, 820, 1186, 1306, 1801, 2101, 2188
TseFI GTSAC 4 cut(s) 686, 1063, 1552, 2183
TseI GCWGC 7 cut(s) 93, 560, 614, 716, 943, 1423, 1814
Tsp45I GTSAC 4 cut(s) 686, 1063, 1552, 2183
TspGWI ACGGA 1 cut(s) 857
TspRI CASTG 8 cut(s) 405, 526, 820, 1186, 1306, 1801, 2101, 2188
Van91I CCANNNNNTGG 1 cut(s) 20
Vha464I CTTAAG 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 1443
XagI CCTNNNNNAGG 1 cut(s) 2214
XapI RAATTY 4 cut(s) 374, 442, 1644, 2047
XceI RCATGY 3 cut(s) 385, 398, 1423
XmnI GAANNNNTTC 3 cut(s) 1321, 1758, 2138
XspI CTAG 5 cut(s) 417, 893, 980, 1763, 2172
ZrmI AGTACT 1 cut(s) 1088
Zsp2I ATGCAT 2 cut(s) 608, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.